AT5G14650

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009157
Physical Location & Seq
Forward (+)
4724407 .. 4726675
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14650.1

Sequence Viewer

Length: 1308 bp
ATGTGTAGACTAACATTGAAATGTCTTTCCCTGAACTTTCTCCTCTTAATCTCCTTATTCTCTTCGAGATTCGGGACTTGTGACGCTAGGTACAGTGTATACTGGAAAGGAAACCGAAGGTCTATCGCCGAAGGGGGAAGTTCCGGAACAATCAATGTGCTTGACCATGGCGCAAAAGGTGACGGGACAAGTGATGACACAAAGGCTTTTGAAGATGCTTGGCAGGTAGCTTGTAAGGTGGCAGCGTCAACATTATTAGTTCCATCTGGTTCTACGTTCCTTGTTGGACCTGTTTCCTTCCTGGGAAAAGAATGTAAAGAGAAAATCGTCTTTCAGTTAGAAGGAAAGATCATAGCTCCAACGAGTGCTAGTGCATGGGGATCAGGTCTCTTGCAATGGATAGAATTCAAAGCACTTCAAGGAATCACTATAAAAGGAAAAGGGATCATCGATGGACGAGGATCAGTATGGTGGAACGACATGATGGGTACAAAAATGCCAAGGACCAAACCAACAGCTTTGAGGTTCTACGGGAGTAACGGTGTTACGGTCAGTGGAATAACCATACAGAACAGCCCTCAAACCCACCTCAAGTTTGATAACTGCATAAGCATCCAAGTTTCTGATTTCACAACTTCATCCCCTGGAGATTCCCCAAACACAGATGGAATCCACCTGCAAAACTCTCAAGATGCCGTTATATATCGCAGCACGCTGGCTTGTGGAGATGACTGTATATCAATTCAAACGGGATGCTCGAATATCAACATACACGATGTAGACTGTGGCCCTGGCCATGGAATTAGCATTGGAGGACTGGGAAAGGACAATACAAAGGCGTGTGTTTCAAACATCACTGTCCGTGATGTCACGATGCATGAAACTACGAATGGTGTTCGAATAAAGTCCTGGCAGGGAGGATCAGGGTCTGTAAAACAAGTTATGTTTTCAAATATTCAAGTCAGCAACGTAGCCAATCCAATAATAATAGATCAATATTACTGCGATGGTGGCGGATGCCACAATGAAACTTCAGCAGTCGCTGTATCAAACATAAACTACATAAACATAAAAGGTACCTACACCAAAGAACCTGTACGCTTTGCCTGCAGTGATAGCTTGCCATGCACAGGAATCTCACTGTCGACCATCGAGCTTAAGCCAGCCACAGGAAAAGCAAGTTCACTTGATCCTTTCTGCTGGAAAGCACACGGTGAACTGAAGACAAAAACTCTTCCACCAATACAATGTTTGAAAACAGAAAAGTCACCGGAAGCTGCAAGTCGGTCTAATAATGATGCTTGCTGA

Protein Analysis

435

Amino Acids

46.48

Weight (kDa)

8.21

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 80 - 388 2e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 684
Acc36I ACCTGC 2 cut(s) 214, 684
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 1076
AccI GTMKAC 4 cut(s) 7, 99, 780, 1145
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 1 cut(s) 1014
AclWI GGATC 5 cut(s) 388, 452, 469, 928, 1184
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 1017, 1241
AdeI CACNNNGTG 1 cut(s) 1214
AfaI GTAC 4 cut(s) 92, 490, 1078, 1098
AfiI CCNNNNNNNGG 3 cut(s) 797, 1130, 1169
AflII CTTAAG 1 cut(s) 1157
AgsI TTSAA 9 cut(s) 19, 212, 409, 419, 746, 849, 951, 959, 1255
AjnI CCWGG 4 cut(s) 300, 643, 790, 908
AluBI AGCT 6 cut(s) 230, 356, 518, 1119, 1156, 1277
AluI AGCT 6 cut(s) 230, 356, 518, 1119, 1156, 1277
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 5 cut(s) 388, 452, 469, 928, 1184
AlwNI CAGNNNCTG 2 cut(s) 929, 1043
Aor13HI TCCGGA 1 cut(s) 143
AoxI GGCC 2 cut(s) 787, 793
ApeKI GCWGC 3 cut(s) 242, 708, 1277
ApoI RAATTY 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 3 cut(s) 287, 504, 788
AsuHPI GGTGA 3 cut(s) 191, 1226, 1260
AsuII TTCGAA 1 cut(s) 898
AvaII GGWCC 2 cut(s) 287, 504
BalI TGGCCA 1 cut(s) 795
BanI GGYRCC 1 cut(s) 1076
BbsI GAAGAC 1 cut(s) 1229
BbvI GCAGC 3 cut(s) 254, 720, 1264
BccI CCATC 6 cut(s) 271, 446, 478, 659, 1001, 1157
BceAI ACGGC 1 cut(s) 680
BciT130I CCWGG 4 cut(s) 302, 645, 792, 910
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 2 cut(s) 87, 369
BfmI CTRYAG 1 cut(s) 1108
BfrI CTTAAG 1 cut(s) 1157
BfuAI ACCTGC 2 cut(s) 214, 684
BisI GCNGC 3 cut(s) 243, 709, 1278
BlsI GCNGC 3 cut(s) 244, 710, 1279
Bme1390I CCNGG 4 cut(s) 302, 645, 792, 910
Bme18I GGWCC 2 cut(s) 287, 504
BmgT120I GGNCC 3 cut(s) 287, 504, 788
BmiI GGNNCC 1 cut(s) 1078
BmrFI CCNGG 4 cut(s) 302, 645, 792, 910
BmrI ACTGGG 1 cut(s) 827
BmsI GCATC 7 cut(s) 205, 621, 682, 743, 864, 1007, 1288
BmuI ACTGGG 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 1229
BpmI CTGGAG 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 898
BpuEI CTTGAG 2 cut(s) 575, 672
Bsa29I ATCGAT 1 cut(s) 450
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 6 cut(s) 166, 301, 500, 643, 790, 796
BsaWI WCCGGW 2 cut(s) 143, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 3 cut(s) 797, 1130, 1169
Bse1I ACTGG 2 cut(s) 107, 822
Bse3DI GCAATG 1 cut(s) 401
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 4 cut(s) 302, 645, 792, 910
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 6 cut(s) 166, 301, 500, 643, 790, 796
BseGI GGATG 4 cut(s) 612, 638, 758, 1022
BseLI CCNNNNNNNGG 3 cut(s) 797, 1130, 1169
BseMI GCAATG 1 cut(s) 401
BseNI ACTGG 2 cut(s) 107, 822
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 3 cut(s) 254, 720, 1264
BshFI GGCC 2 cut(s) 789, 795
BshNI GGYRCC 1 cut(s) 1076
BshVI ATCGAT 1 cut(s) 450
BsiSI CCGG 2 cut(s) 144, 1271
BslFI GGGAC 2 cut(s) 88, 199
BslI CCNNNNNNNGG 3 cut(s) 797, 1130, 1169
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 2 cut(s) 88, 199
BsnI GGCC 2 cut(s) 789, 795
Bso31I GGTCTC 1 cut(s) 392
Bsp119I TTCGAA 1 cut(s) 898
Bsp13I TCCGGA 1 cut(s) 143
Bsp143I GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
Bsp19I CCATGG 2 cut(s) 166, 796
BspACI CCGC 1 cut(s) 1014
BspANI GGCC 2 cut(s) 789, 795
BspDI ATCGAT 1 cut(s) 450
BspEI TCCGGA 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 1078
BspMAI CTGCAG 1 cut(s) 1112
BspMI ACCTGC 2 cut(s) 214, 684
BspPI GGATC 5 cut(s) 388, 452, 469, 928, 1184
BspT104I TTCGAA 1 cut(s) 898
BspT107I GGYRCC 1 cut(s) 1076
BspTI CTTAAG 1 cut(s) 1157
BspTNI GGTCTC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 401
BsrI ACTGG 2 cut(s) 107, 822
BssECI CCNNGG 6 cut(s) 166, 301, 500, 643, 790, 796
BssMI GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
BssNAI GTATAC 1 cut(s) 100
BssT1I CCWWGG 3 cut(s) 166, 500, 796
Bst1107I GTATAC 1 cut(s) 100
Bst2UI CCWGG 4 cut(s) 302, 645, 792, 910
Bst4CI ACNGT 8 cut(s) 95, 542, 550, 734, 785, 859, 1143, 1214
Bst6I CTCTTC 2 cut(s) 67, 1239
BstAFI CTTAAG 1 cut(s) 1157
BstBI TTCGAA 1 cut(s) 898
BstC8I GCNNGC 6 cut(s) 713, 717, 1108, 1121, 1164, 1303
BstDSI CCRYGG 2 cut(s) 166, 796
BstF5I GGATG 4 cut(s) 612, 638, 758, 1022
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 7 cut(s) 351, 383, 447, 464, 923, 994, 1192
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
BstMWI GCNNNNNNNGC 4 cut(s) 1011, 1107, 1116, 1125
BstNI CCWGG 4 cut(s) 302, 645, 792, 910
BstSCI CCNGG 4 cut(s) 300, 643, 790, 908
BstSFI CTRYAG 1 cut(s) 1108
BstV1I GCAGC 3 cut(s) 254, 720, 1264
BstV2I GAAGAC 1 cut(s) 1229
BstZ17I GTATAC 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 2 cut(s) 789, 795
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 2 cut(s) 166, 796
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 4 cut(s) 612, 638, 758, 1022
BtsI GCAGTG 1 cut(s) 1117
BtsIMutI CAGTG 5 cut(s) 100, 559, 855, 1117, 1139
BveI ACCTGC 2 cut(s) 214, 684
Cac8I GCNNGC 6 cut(s) 713, 717, 1108, 1121, 1164, 1303
CaiI CAGNNNCTG 2 cut(s) 929, 1043
CfoI GCGC 1 cut(s) 173
Cfr13I GGNCC 3 cut(s) 287, 504, 788
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 2 cut(s) 92, 234
Csp6I GTAC 4 cut(s) 91, 489, 1077, 1097
CviAII CATG 6 cut(s) 167, 375, 481, 797, 878, 1125
CviQI GTAC 4 cut(s) 91, 489, 1077, 1097
DpnI GATC 7 cut(s) 350, 382, 446, 463, 922, 993, 1191
DpnII GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
DraIII CACNNNGTG 1 cut(s) 1214
EaeI YGGCCR 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 67, 1239
EarI CTCTTC 2 cut(s) 67, 1239
EciI GGCGGA 1 cut(s) 1029
Eco130I CCWWGG 3 cut(s) 166, 500, 796
Eco31I GGTCTC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 287, 504
Eco57I CTGAAG 2 cut(s) 1017, 1241
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 4 cut(s) 300, 643, 790, 908
EcoT14I CCWWGG 3 cut(s) 166, 500, 796
EcoT22I ATGCAT 1 cut(s) 879
ErhI CCWWGG 3 cut(s) 166, 500, 796
FaeI CATG 6 cut(s) 170, 378, 484, 800, 881, 1128
FaqI GGGAC 2 cut(s) 88, 199
FatI CATG 6 cut(s) 166, 374, 480, 796, 877, 1124
FblI GTMKAC 4 cut(s) 7, 99, 780, 1145
Fnu4HI GCNGC 3 cut(s) 243, 709, 1278
FokI GGATG 4 cut(s) 599, 625, 765, 1029
Fsp4HI GCNGC 3 cut(s) 243, 709, 1278
FspBI CTAG 2 cut(s) 87, 369
GlaI GCGC 1 cut(s) 172
GluI GCNGC 3 cut(s) 243, 709, 1278
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 2 cut(s) 789, 795
HapII CCGG 2 cut(s) 144, 1271
HgaI GACGC 2 cut(s) 92, 234
HhaI GCGC 1 cut(s) 173
Hin1II CATG 6 cut(s) 170, 378, 484, 800, 881, 1128
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 2 cut(s) 249, 1146
HindII GTYRAC 2 cut(s) 249, 1146
HinfI GANTC 5 cut(s) 69, 423, 650, 669, 1134
HpaII CCGG 2 cut(s) 144, 1271
HphI GGTGA 3 cut(s) 191, 1226, 1260
Hpy166II GTNNAC 7 cut(s) 8, 100, 249, 781, 1146, 1184, 1217
Hpy188I TCNGA 1 cut(s) 625
Hpy188III TCNNGA 5 cut(s) 66, 73, 144, 689, 871
Hpy8I GTNNAC 7 cut(s) 8, 100, 249, 781, 1146, 1184, 1217
HpyAV CCTTC 4 cut(s) 111, 125, 307, 335
HpyCH4III ACNGT 8 cut(s) 95, 542, 550, 734, 785, 859, 1143, 1214
HpyCH4IV ACGT 2 cut(s) 275, 969
HpyCH4V TGCA 8 cut(s) 374, 394, 606, 679, 877, 1110, 1128, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 1011, 1107, 1116, 1125
HpySE526I ACGT 2 cut(s) 275, 969
Hsp92II CATG 6 cut(s) 170, 378, 484, 800, 881, 1128
HspAI GCGC 1 cut(s) 171
Kpn2I TCCGGA 1 cut(s) 143
KpnI GGTACC 1 cut(s) 1080
Kzo9I GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 3 cut(s) 254, 720, 1264
LweI GCATC 7 cut(s) 205, 621, 682, 743, 864, 1007, 1288
MaeI CTAG 2 cut(s) 87, 369
MaeII ACGT 2 cut(s) 275, 969
MaeIII GTNAC 6 cut(s) 80, 179, 536, 544, 868, 1266
MalI GATC 7 cut(s) 350, 382, 446, 463, 922, 993, 1191
MboI GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
MboII GAAGA 4 cut(s) 54, 224, 1226, 1234
MlsI TGGCCA 1 cut(s) 795
MluCI AATT 3 cut(s) 404, 741, 801
MluNI TGGCCA 1 cut(s) 795
MmeI TCCRAC 2 cut(s) 265, 383
MnlI CCTC 7 cut(s) 53, 452, 516, 588, 599, 806, 911
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 879
MroI TCCGGA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 795
MseI TTAA 2 cut(s) 47, 1158
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 795
MspCI CTTAAG 1 cut(s) 1157
MspI CCGG 2 cut(s) 144, 1271
MspR9I CCNGG 4 cut(s) 302, 645, 792, 910
MvaI CCWGG 4 cut(s) 302, 645, 792, 910
MwoI GCNNNNNNNGC 4 cut(s) 1011, 1107, 1116, 1125
NcoI CCATGG 2 cut(s) 166, 796
NdeII GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
NlaIII CATG 6 cut(s) 170, 378, 484, 800, 881, 1128
NlaIV GGNNCC 1 cut(s) 1078
NmuCI GTSAC 4 cut(s) 80, 179, 868, 1266
NsiI ATGCAT 1 cut(s) 879
NspV TTCGAA 1 cut(s) 898
PaqCI CACCTGC 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 755
PfeI GAWTC 5 cut(s) 69, 423, 650, 669, 1134
PkrI GCNGC 3 cut(s) 244, 710, 1279
Psp6I CCWGG 4 cut(s) 300, 643, 790, 908
PspGI CCWGG 4 cut(s) 300, 643, 790, 908
PspN4I GGNNCC 1 cut(s) 1078
PspPI GGNCC 3 cut(s) 287, 504, 788
PstI CTGCAG 1 cut(s) 1112
PstNI CAGNNNCTG 2 cut(s) 929, 1043
RsaI GTAC 4 cut(s) 92, 490, 1078, 1098
RsaNI GTAC 4 cut(s) 91, 489, 1077, 1097
RseI CAYNNNNRTG 1 cut(s) 19
SalI GTCGAC 1 cut(s) 1144
SaqAI TTAA 2 cut(s) 47, 1158
SatI GCNGC 3 cut(s) 243, 709, 1278
Sau3AI GATC 7 cut(s) 348, 380, 444, 461, 920, 991, 1189
Sau96I GGNCC 3 cut(s) 287, 504, 788
ScrFI CCNGG 4 cut(s) 302, 645, 792, 910
SfaNI GCATC 7 cut(s) 205, 621, 682, 743, 864, 1007, 1288
SfcI CTRYAG 1 cut(s) 1108
SfuI TTCGAA 1 cut(s) 898
SinI GGWCC 2 cut(s) 287, 504
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 3 cut(s) 590, 687, 1157
SmoI CTYRAG 3 cut(s) 590, 687, 1157
Sse9I AATT 3 cut(s) 404, 741, 801
SsiI CCGC 1 cut(s) 1014
SspI AATATT 2 cut(s) 955, 998
SspMI CTAG 2 cut(s) 87, 369
StyD4I CCNGG 4 cut(s) 300, 643, 790, 908
StyI CCWWGG 3 cut(s) 166, 500, 796
TaaI ACNGT 8 cut(s) 95, 542, 550, 734, 785, 859, 1143, 1214
TaiI ACGT 2 cut(s) 278, 972
TaqI TCGA 6 cut(s) 65, 450, 758, 898, 1145, 1152
TaqII GACCGA 1 cut(s) 1275
TasI AATT 3 cut(s) 404, 741, 801
TfiI GAWTC 5 cut(s) 69, 423, 650, 669, 1134
Tru1I TTAA 2 cut(s) 47, 1158
Tru9I TTAA 2 cut(s) 47, 1158
TscAI CASTG 5 cut(s) 100, 559, 862, 1117, 1146
TseFI GTSAC 4 cut(s) 80, 179, 868, 1266
TseI GCWGC 3 cut(s) 242, 708, 1277
Tsp45I GTSAC 4 cut(s) 80, 179, 868, 1266
TspDTI ATGAA 3 cut(s) 627, 894, 1041
TspGWI ACGGA 1 cut(s) 851
TspRI CASTG 5 cut(s) 100, 559, 862, 1117, 1146
Vha464I CTTAAG 1 cut(s) 1157
VpaK11BI GGWCC 2 cut(s) 287, 504
XapI RAATTY 1 cut(s) 404
XmiI GTMKAC 4 cut(s) 7, 99, 780, 1145
XspI CTAG 2 cut(s) 87, 369
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.