Rh2AG633800

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009157
Physical Location & Seq
Reverse (-)
85967482 .. 85970738
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG633800.1

Sequence Viewer

Length: 1056 bp
ATGCAGTTAGATGGAAAAATCATAGCCCTGACAGGCGCGGGAGCGTGGGGTTCAGGCCTTCTACAATGGCTTGAGTTCACAAAGCTTCAAAGACTTACCATCAGAGGAAAAGGTGTGATTGATGGACAAGGTGCAGTTTGGTGGAATGACTCGCCCACATACGATCCTACAGATGAAACAGCAGACAACAGTACAGAAGATTCGCAGACCGTAGACAGTTACAGTGGAAAAATGCCAAGCACAAAACCAACAGCGCTTAGGTTCTATGGAAGCAACGGTGTGACAGTCACTGGCATAACAATTCAAAACAGCCCTCAAACCCACCTCAAGTTTGACTCCTGCACTAATGTACAAGTTTCTGCCGTAACTATTTCATCCCCTGGCAACAGCCCCAACACTGATGGAATCCACCTTCAAAACTCCCAGGATGTGGTCATTTCCGGCGCAAGCCTTGCTTGTGGAGATGATTGTATATCAATACAAACTGGCTGCTCAAACGTATTCATACACAATGTAAACTGTGGACCTGGACATGGAGTCAGCATTGGAGGGCTAGGAAAGGATGGCACCAAAGCTTGTGTGTCAAACATCACCGTCCGAGATGTTAAAATGCAAAATACACTGAACGGAGTCAGAATAAAGACCTGGCAGGGAGGATCAGGGTCAGTAAAAAATGTCATGTTCGCAAACATTCAAGTTTCAGAAGTTGAATTCCCTATCATGATTGACCAATACTACTGTGACAAGGGGCATTGCGCAAACAAAACCTCAGCTGTGGCAGTTTCGGGTATAAACTACGTAAACATTCAAGGAACCTATACATCAAAACCTGCACAATTTGCATGCAGTGACAGCATACCATGTACTGGTGTATCCCTAGACACCATAGACCTAAAATCGGTTCAATCAGGGAGGCACTTGTCTGACCCTTTCTGTTGGGAGGCATATGGAGACCTCAAGACAAACACTGTCCCCCCAATTGATTGCTTGATGAAAGGAAAGCCATCTTCAAGCAGCAGCACAGTCCAGTCTCAAGGTGGTGGCTATTCTTGCTAA

Protein Analysis

351

Amino Acids

37.06

Weight (kDa)

5.64

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 18 - 301 1.4e-73 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 757
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 2 cut(s) 430, 866
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 38
AciI CCGC 1 cut(s) 38
AclWI GGATC 2 cut(s) 158, 664
AcsI RAATTY 1 cut(s) 710
AfaI GTAC 3 cut(s) 193, 351, 865
AfeI AGCGCT 1 cut(s) 255
AfiI CCNNNNNNNGG 4 cut(s) 430, 533, 866, 898
AgsI TTSAA 8 cut(s) 89, 305, 416, 695, 710, 809, 905, 1011
AhdI GACNNNNNGTC 1 cut(s) 536
AjnI CCWGG 4 cut(s) 379, 423, 526, 644
AluBI AGCT 3 cut(s) 85, 575, 773
AluI AGCT 3 cut(s) 85, 575, 773
Alw26I GTCTC 2 cut(s) 945, 1035
AlwI GGATC 2 cut(s) 158, 664
AlwNI CAGNNNCTG 1 cut(s) 290
Aor51HI AGCGCT 1 cut(s) 255
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 489, 1014, 1017
ApoI RAATTY 1 cut(s) 710
AspLEI GCGC 4 cut(s) 38, 256, 446, 758
AspS9I GGNCC 1 cut(s) 524
AsuHPI GGTGA 1 cut(s) 583
AvaII GGWCC 1 cut(s) 524
BaeI ACNNNNGTAYC 2 cut(s) 855, 888
BanI GGYRCC 1 cut(s) 566
BbvCI CCTCAGC 1 cut(s) 769
BbvI GCAGC 3 cut(s) 476, 1026, 1029
BccI CCATC 6 cut(s) 5, 107, 116, 395, 557, 1012
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 4 cut(s) 381, 425, 528, 646
BciVI GTATCC 1 cut(s) 883
BcoDI GTCTC 2 cut(s) 945, 1035
BfaI CTAG 2 cut(s) 554, 878
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 838
BfuI GTATCC 1 cut(s) 883
BisI GCNGC 3 cut(s) 490, 1015, 1018
BlsI GCNGC 3 cut(s) 491, 1016, 1019
Bme1390I CCNGG 4 cut(s) 381, 425, 528, 646
Bme18I GGWCC 1 cut(s) 524
BmeRI GACNNNNNGTC 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 2 cut(s) 568, 814
BmrFI CCNGG 4 cut(s) 381, 425, 528, 646
Bpu10I CCTNAGC 2 cut(s) 257, 769
BpuEI CTTGAG 4 cut(s) 92, 311, 941, 1017
BsaAI YACGTR 1 cut(s) 799
BsaI GGTCTC 1 cut(s) 945
BsaJI CCNNGG 2 cut(s) 379, 423
Bsc4I CCNNNNNNNGG 4 cut(s) 430, 533, 866, 898
Bse1I ACTGG 4 cut(s) 295, 490, 871, 1027
Bse3DI GCAATG 1 cut(s) 751
BseBI CCWGG 4 cut(s) 381, 425, 528, 646
BseDI CCNNGG 2 cut(s) 379, 423
BseGI GGATG 3 cut(s) 374, 433, 568
BseLI CCNNNNNNNGG 4 cut(s) 430, 533, 866, 898
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 4 cut(s) 295, 490, 871, 1027
BseXI GCAGC 3 cut(s) 476, 1026, 1029
BsgI GTGCAG 3 cut(s) 153, 325, 816
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 566
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 956
BslI CCNNNNNNNGG 4 cut(s) 430, 533, 866, 898
BsmAI GTCTC 2 cut(s) 945, 1035
BsmFI GGGAC 1 cut(s) 956
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 945
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 2 cut(s) 163, 656
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 782
BspFNI CGCG 1 cut(s) 38
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 2 cut(s) 568, 814
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 2 cut(s) 158, 664
BspT107I GGYRCC 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 945
BsrDI GCAATG 1 cut(s) 751
BsrGI TGTACA 1 cut(s) 349
BsrI ACTGG 4 cut(s) 295, 490, 871, 1027
BssECI CCNNGG 2 cut(s) 379, 423
BssMI GATC 2 cut(s) 163, 656
Bst2UI CCWGG 4 cut(s) 381, 425, 528, 646
BstAPI GCANNNNNTGC 2 cut(s) 452, 839
BstAUI TGTACA 1 cut(s) 349
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 2 cut(s) 448, 844
BstDEI CTNAG 2 cut(s) 257, 769
BstF5I GGATG 3 cut(s) 374, 433, 568
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 4 cut(s) 38, 256, 446, 758
BstKTI GATC 2 cut(s) 166, 659
BstMAI GTCTC 2 cut(s) 945, 1035
BstMBI GATC 2 cut(s) 163, 656
BstMWI GCNNNNNNNGC 4 cut(s) 452, 839, 852, 1050
BstNI CCWGG 4 cut(s) 381, 425, 528, 646
BstNSI RCATGY 1 cut(s) 846
BstSCI CCNGG 4 cut(s) 379, 423, 526, 644
BstSFI CTRYAG 1 cut(s) 168
BstSNI TACGTA 1 cut(s) 799
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 3 cut(s) 476, 1026, 1029
BsuI GTATCC 1 cut(s) 883
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 3 cut(s) 374, 433, 568
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 6 cut(s) 229, 288, 396, 620, 853, 966
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 2 cut(s) 448, 844
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 720
CfoI GCGC 4 cut(s) 38, 256, 446, 758
Cfr13I GGNCC 1 cut(s) 524
Csp6I GTAC 3 cut(s) 192, 350, 864
CviAII CATG 5 cut(s) 533, 679, 721, 843, 861
CviQI GTAC 3 cut(s) 192, 350, 864
DdeI CTNAG 2 cut(s) 257, 769
DpnI GATC 2 cut(s) 165, 658
DpnII GATC 2 cut(s) 163, 656
DriI GACNNNNNGTC 1 cut(s) 536
Eam1105I GACNNNNNGTC 1 cut(s) 536
Eco105I TACGTA 1 cut(s) 799
Eco147I AGGCCT 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 945
Eco47I GGWCC 1 cut(s) 524
Eco47III AGCGCT 1 cut(s) 255
EcoRI GAATTC 1 cut(s) 710
EcoRII CCWGG 4 cut(s) 379, 423, 526, 644
FaeI CATG 5 cut(s) 536, 682, 724, 846, 864
FalI AAGNNNNNCTT 2 cut(s) 439, 471
FaqI GGGAC 1 cut(s) 956
FatI CATG 5 cut(s) 532, 678, 720, 842, 860
FauI CCCGC 1 cut(s) 31
FauNDI CATATG 1 cut(s) 946
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 490, 1015, 1018
FokI GGATG 3 cut(s) 361, 440, 575
Fsp4HI GCNGC 3 cut(s) 490, 1015, 1018
FspBI CTAG 2 cut(s) 554, 878
FspI TGCGCA 1 cut(s) 757
GlaI GCGC 4 cut(s) 37, 255, 445, 757
GluI GCNGC 3 cut(s) 490, 1015, 1018
HaeII RGCGCY 1 cut(s) 257
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 441
HhaI GCGC 4 cut(s) 38, 256, 446, 758
Hin1II CATG 5 cut(s) 536, 682, 724, 846, 864
Hin6I GCGC 4 cut(s) 36, 254, 444, 756
HinP1I GCGC 4 cut(s) 36, 254, 444, 756
HindIII AAGCTT 2 cut(s) 83, 573
HinfI GANTC 6 cut(s) 149, 200, 335, 405, 537, 630
HpaII CCGG 1 cut(s) 441
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 5 cut(s) 78, 214, 517, 524, 802
Hpy188I TCNGA 5 cut(s) 104, 599, 635, 703, 925
Hpy188III TCNNGA 2 cut(s) 721, 958
Hpy8I GTNNAC 5 cut(s) 78, 214, 517, 524, 802
HpyAV CCTTC 2 cut(s) 68, 422
HpyCH4IV ACGT 2 cut(s) 498, 798
HpyCH4V TGCA 7 cut(s) 4, 134, 342, 613, 833, 842, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 452, 839, 852, 1050
HpyF3I CTNAG 2 cut(s) 257, 769
HpySE526I ACGT 2 cut(s) 498, 798
Hsp92II CATG 5 cut(s) 536, 682, 724, 846, 864
HspAI GCGC 4 cut(s) 36, 254, 444, 756
Kzo9I GATC 2 cut(s) 163, 656
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 3 cut(s) 476, 1026, 1029
MaeI CTAG 2 cut(s) 554, 878
MaeII ACGT 2 cut(s) 498, 798
MaeIII GTNAC 6 cut(s) 218, 280, 286, 364, 740, 848
MalI GATC 2 cut(s) 165, 658
MboI GATC 2 cut(s) 163, 656
MboII GAAGA 2 cut(s) 209, 999
MfeI CAATTG 1 cut(s) 978
MluCI AATT 4 cut(s) 300, 710, 836, 978
MlyI GAGTC 4 cut(s) 143, 329, 546, 639
MnlI CCTC 9 cut(s) 98, 324, 335, 542, 647, 778, 906, 934, 965
MseI TTAA 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 4 cut(s) 381, 425, 528, 646
MunI CAATTG 1 cut(s) 978
MvaI CCWGG 4 cut(s) 381, 425, 528, 646
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 4 cut(s) 452, 839, 852, 1050
NdeI CATATG 1 cut(s) 946
NdeII GATC 2 cut(s) 163, 656
NlaIII CATG 5 cut(s) 536, 682, 724, 846, 864
NlaIV GGNNCC 2 cut(s) 568, 814
NmuCI GTSAC 4 cut(s) 280, 286, 740, 848
NsbI TGCGCA 1 cut(s) 757
NspI RCATGY 1 cut(s) 846
PaeI GCATGC 1 cut(s) 846
PagI TCATGA 1 cut(s) 720
PceI AGGCCT 1 cut(s) 57
PfeI GAWTC 2 cut(s) 200, 405
PflMI CCANNNNNTGG 2 cut(s) 430, 866
PkrI GCNGC 3 cut(s) 491, 1016, 1019
PleI GAGTC 4 cut(s) 143, 329, 545, 638
PpsI GAGTC 4 cut(s) 143, 329, 545, 638
Ppu21I YACGTR 1 cut(s) 799
Psp6I CCWGG 4 cut(s) 379, 423, 526, 644
PspGI CCWGG 4 cut(s) 379, 423, 526, 644
PspN4I GGNNCC 2 cut(s) 568, 814
PspPI GGNCC 1 cut(s) 524
PstNI CAGNNNCTG 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 3 cut(s) 193, 351, 865
RsaNI GTAC 3 cut(s) 192, 350, 864
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 3 cut(s) 490, 1015, 1018
Sau3AI GATC 2 cut(s) 163, 656
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 4 cut(s) 143, 329, 546, 639
ScrFI CCNGG 4 cut(s) 381, 425, 528, 646
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 1 cut(s) 524
SmlI CTYRAG 4 cut(s) 71, 326, 956, 1032
SmoI CTYRAG 4 cut(s) 71, 326, 956, 1032
SnaBI TACGTA 1 cut(s) 799
SphI GCATGC 1 cut(s) 846
Sse9I AATT 4 cut(s) 300, 710, 836, 978
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 1 cut(s) 38
SspMI CTAG 2 cut(s) 554, 878
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 4 cut(s) 379, 423, 526, 644
TaiI ACGT 2 cut(s) 501, 801
TasI AATT 4 cut(s) 300, 710, 836, 978
TatI WGTACW 3 cut(s) 191, 349, 863
TfiI GAWTC 2 cut(s) 200, 405
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 6 cut(s) 229, 295, 403, 627, 853, 973
TseFI GTSAC 4 cut(s) 280, 286, 740, 848
TseI GCWGC 3 cut(s) 489, 1014, 1017
Tsp45I GTSAC 4 cut(s) 280, 286, 740, 848
TspDTI ATGAA 4 cut(s) 189, 363, 493, 1007
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 6 cut(s) 229, 295, 403, 627, 853, 973
Van91I CCANNNNNTGG 2 cut(s) 430, 866
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 710
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 554, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.