Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009157

Physical Location & Seq

Genomic Coordinates Forward (+)

1852886 .. 1854512

Gene Structure 1627 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1030100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGAGGACGTTTAACCTAAAAACCCTCTTGTTCTTTGTTCTCATTGCGTTTATTATCTCGTTTTCGAGTTTTTTCGAACCGTGTGATGCTCGAGAGGGAGGCAATAAGCAGCACTGGAGGAAGAAACAAAAGGGTTATGGTGGTGATCATCGCCGCGCTGCGTCGAGGGGAGACAGTTACATCACAACGGACGAAACTGCGGTGACACTTGGAAAAAAGAGCGGCGGGGGTTCGAACGTGTTTAATGTGTTGGACTATGGTGCTAAAGGTGATGGAAAAGCTGATGACACCAAGGCGTTCGAGGCGGCGTGGGCAGCAGCTTGCAAAGTGCAGGCATCAACTATAAGTGTTCCTTCCGGGTCTGTGTTCCTTGTCAAACCAATCTCTTTCTCTGGCCCTAACTGCGAAGAAAATATCGTGTTTCAGTTAGATGGTAAAATCATAGCTCCAACAAGCTCAGGGGATTGGGGTTCAGGTCTTTTACAATGGATTGAATTCACAAAACTAAAAGGGATTTCGATCAAAGGCACCGGAGTCATTGATGGACAAGGCTCGGTTTGGTGGAATGACTCGCCGAAATACAATCCTGCAAACGAATCAACGGATAGCAGTCTCGATGATTTGGCCACAGAAAGTTACAGTGGGAAAATGCCAAGCACCAAACCAACGGCTCTTAGGTTCTATGGAAGCGACCTCGTTAGTGTCACTGGCATAACACTTCAAAACAGCCCTCAGGCGCACCTCAAGTTCGACGCCTGCACAAATGTTCAGGTTTCCGGTATAAGTATTTCATCCCCGGGCAACAGCCCCAACACGGATGGAATCCACCTCCAAAACTCCCAAAATGCTGTCATTTCCGCCAGCAGTATCGCCTGTGGTAATTTTTCAGAATCCGGTCTTCGACAAGGGCAACCTTGTCCAAAAACCTGGGTGAAAAAAACTGACAGAAGTTGTAAATCACAACATAAAATTAACTACGTAACCTGTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0009664 GO:0009825 GO:0009827 GO:0009831 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0016798 GO:0030312 GO:0040007 GO:0042545 GO:0042547 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

331

Amino Acids

35.38

Weight (kDa)

8.68

Isoelectric Point (pI)

41.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	81 - 128	4.5e-07	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	110 - 293	6.8e-27	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14650 AT5G14650
fragaria_vesca	FvH4_6g50960 FvH4_6g50960 FvH4_6g50960 FvH4_6g50960 FvH4_6g50960
malus_domestica	MD09G1030100.v1.1 MD09G1030200.v1.1 MD17G1032000.v1.1
prunus_persica	Prupe.3G287200_v2.0.a1
pyrus_communis	pycom111g02420
rosa_chinensis	RchiOBHm_Chr2g0172361
rosa_laevigata	RLG00000022107
rosa_multiflora	Rmu_sc0000467.1_g000009
rosa_roxburghii	Rroxscaffold_2G00079950
rosa_rugosa	Rorug02G0558800
rosa_samantha	Rh2AG633800 Rh2BG647600 Rh2CG614600 Rh2DG662400
rosa_wichuraiana	Rw2G052500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	527
AccBSI	CCGCTC	1 cut(s)	222
AccII	CGCG	1 cut(s)	156
AciI	CCGC	6 cut(s)	154, 200, 222, 225, 305, 858
AcoI	YGGCCR	1 cut(s)	624
AcsI	RAATTY	1 cut(s)	494
AcyI	GRCGYC	1 cut(s)	753
AfiI	CCNNNNNNNGG	1 cut(s)	814
AflIII	ACRYGT	1 cut(s)	237
AgsI	TTSAA	2 cut(s)	494, 722
AjnI	CCWGG	1 cut(s)	926
AluBI	AGCT	4 cut(s)	281, 320, 446, 456
AluI	AGCT	4 cut(s)	281, 320, 446, 456
Alw26I	GTCTC	2 cut(s)	165, 617
Ama87I	CYCGRG	2 cut(s)	90, 796
AoxI	GGCC	2 cut(s)	394, 624
ApeKI	GCWGC	4 cut(s)	109, 158, 314, 317
ApoI	RAATTY	1 cut(s)	494
AspLEI	GCGC	2 cut(s)	158, 739
AspS9I	GGNCC	1 cut(s)	395
AsuC2I	CCSGG	3 cut(s)	358, 797, 798
AsuHPI	GGTGA	4 cut(s)	155, 214, 281, 943
AsuII	TTCGAA	2 cut(s)	75, 233
AvaI	CYCGRG	2 cut(s)	90, 796
AxyI	CCTNAGG	1 cut(s)	732
BalI	TGGCCA	1 cut(s)	626
BanI	GGYRCC	1 cut(s)	527
BbsI	GAAGAC	1 cut(s)	890
BbvI	GCAGC	4 cut(s)	121, 145, 326, 329
BccI	CCATC	4 cut(s)	266, 425, 536, 812
BceAI	ACGGC	1 cut(s)	684
BciT130I	CCWGG	1 cut(s)	928
BclI	TGATCA	1 cut(s)	145
BcnI	CCSGG	3 cut(s)	358, 797, 798
BcoDI	GTCTC	2 cut(s)	165, 617
BisI	GCNGC	7 cut(s)	110, 154, 159, 223, 306, 315, 318
BlsI	GCNGC	7 cut(s)	111, 155, 160, 224, 307, 316, 319
Bme1390I	CCNGG	4 cut(s)	358, 797, 798, 928
BmeT110I	CYCGRG	2 cut(s)	90, 796
BmgT120I	GGNCC	1 cut(s)	395
BmiI	GGNNCC	1 cut(s)	529
BmrFI	CCNGG	4 cut(s)	358, 797, 798, 928
BmsI	GCATC	2 cut(s)	76, 344
BpiI	GAAGAC	1 cut(s)	890
BpmI	CTGGAG	1 cut(s)	136
Bpu10I	CCTNAGC	1 cut(s)	457
Bpu14I	TTCGAA	2 cut(s)	75, 233
BpuEI	CTTGAG	1 cut(s)	728
BpuMI	CCSGG	3 cut(s)	358, 797, 798
BsaAI	YACGTR	1 cut(s)	979
BsaBI	GATNNNNATC	1 cut(s)	518
BsaHI	GRCGYC	1 cut(s)	753
BsaJI	CCNNGG	4 cut(s)	291, 795, 796, 927
BsaWI	WCCGGW	3 cut(s)	530, 776, 893
Bsc4I	CCNNNNNNNGG	1 cut(s)	814
Bse1I	ACTGG	2 cut(s)	119, 712
Bse21I	CCTNAGG	1 cut(s)	732
Bse3DI	GCAATG	1 cut(s)	42
Bse8I	GATNNNNATC	1 cut(s)	518
BseBI	CCWGG	1 cut(s)	928
BseDI	CCNNGG	4 cut(s)	291, 795, 796, 927
BseGI	GGATG	2 cut(s)	791, 823
BseJI	GATNNNNATC	1 cut(s)	518
BseLI	CCNNNNNNNGG	1 cut(s)	814
BseMI	GCAATG	1 cut(s)	42
BseMII	CTCAG	2 cut(s)	471, 746
BseNI	ACTGG	2 cut(s)	119, 712
BseXI	GCAGC	4 cut(s)	121, 145, 326, 329
BsgI	GTGCAG	2 cut(s)	350, 742
Bsh1236I	CGCG	1 cut(s)	156
BshFI	GGCC	2 cut(s)	396, 626
BshNI	GGYRCC	1 cut(s)	527
BsiHKCI	CYCGRG	2 cut(s)	90, 796
BsiSI	CCGG	5 cut(s)	357, 531, 777, 797, 894
BslI	CCNNNNNNNGG	1 cut(s)	814
BsmAI	GTCTC	2 cut(s)	165, 617
BsnI	GGCC	2 cut(s)	396, 626
BsoBI	CYCGRG	2 cut(s)	90, 796
Bsp119I	TTCGAA	2 cut(s)	75, 233
Bsp143I	GATC	2 cut(s)	145, 519
BspACI	CCGC	6 cut(s)	154, 200, 222, 225, 305, 858
BspANI	GGCC	2 cut(s)	396, 626
BspCNI	CTCAG	2 cut(s)	470, 745
BspFNI	CGCG	1 cut(s)	156
BspLI	GGNNCC	1 cut(s)	529
BspT104I	TTCGAA	2 cut(s)	75, 233
BspT107I	GGYRCC	1 cut(s)	527
BsrBI	CCGCTC	1 cut(s)	222
BsrDI	GCAATG	1 cut(s)	42
BsrI	ACTGG	2 cut(s)	119, 712
BssECI	CCNNGG	4 cut(s)	291, 795, 796, 927
BssMI	GATC	2 cut(s)	145, 519
BssNI	GRCGYC	1 cut(s)	753
BssT1I	CCWWGG	1 cut(s)	291
Bst2UI	CCWGG	1 cut(s)	928
Bst4CI	ACNGT	3 cut(s)	81, 176, 641
BstACI	GRCGYC	1 cut(s)	753
BstBAI	YACGTR	1 cut(s)	979
BstBI	TTCGAA	2 cut(s)	75, 233
BstC8I	GCNNGC	4 cut(s)	322, 333, 757, 862
BstDEI	CTNAG	3 cut(s)	457, 674, 732
BstF5I	GGATG	2 cut(s)	791, 823
BstFNI	CGCG	1 cut(s)	156
BstHHI	GCGC	2 cut(s)	158, 739
BstKTI	GATC	2 cut(s)	148, 522
BstMAI	GTCTC	2 cut(s)	165, 617
BstMBI	GATC	2 cut(s)	145, 519
BstMWI	GCNNNNNNNGC	4 cut(s)	302, 311, 314, 402
BstNI	CCWGG	1 cut(s)	928
BstSCI	CCNGG	4 cut(s)	356, 795, 796, 926
BstSNI	TACGTA	1 cut(s)	979
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	4 cut(s)	121, 145, 326, 329
BstV2I	GAAGAC	1 cut(s)	890
BstXI	CCANNNNNNTGG	1 cut(s)	927
Bsu36I	CCTNAGG	1 cut(s)	732
BsuRI	GGCC	2 cut(s)	396, 626
BtgZI	GCGATG	1 cut(s)	134
BtsCI	GGATG	2 cut(s)	791, 823
BtsIMutI	CAGTG	3 cut(s)	112, 646, 705
Cac8I	GCNNGC	4 cut(s)	322, 333, 757, 862
CfoI	GCGC	2 cut(s)	158, 739
Cfr13I	GGNCC	1 cut(s)	395
Cfr9I	CCCGGG	1 cut(s)	796
CseI	GACGC	2 cut(s)	150, 761
DdeI	CTNAG	3 cut(s)	457, 674, 732
DpnI	GATC	2 cut(s)	147, 521
DpnII	GATC	2 cut(s)	145, 519
EaeI	YGGCCR	1 cut(s)	624
EciI	GGCGGA	1 cut(s)	847
Eco105I	TACGTA	1 cut(s)	979
Eco130I	CCWWGG	1 cut(s)	291
Eco81I	CCTNAGG	1 cut(s)	732
Eco88I	CYCGRG	2 cut(s)	90, 796
EcoRI	GAATTC	1 cut(s)	494
EcoRII	CCWGG	1 cut(s)	926
EcoT14I	CCWWGG	1 cut(s)	291
ErhI	CCWWGG	1 cut(s)	291
FaiI	YATR	9 cut(s)	138, 258, 344, 443, 684, 713, 782, 966, 991
FalI	AAGNNNNNCTT	2 cut(s)	337, 369
FauI	CCCGC	1 cut(s)	218
FbaI	TGATCA	1 cut(s)	145
Fnu4HI	GCNGC	7 cut(s)	110, 154, 159, 223, 306, 315, 318
FokI	GGATG	2 cut(s)	778, 830
Fsp4HI	GCNGC	7 cut(s)	110, 154, 159, 223, 306, 315, 318
GlaI	GCGC	2 cut(s)	157, 738
GluI	GCNGC	7 cut(s)	110, 154, 159, 223, 306, 315, 318
GsuI	CTGGAG	1 cut(s)	136
HaeIII	GGCC	2 cut(s)	396, 626
HapII	CCGG	5 cut(s)	357, 531, 777, 797, 894
HgaI	GACGC	2 cut(s)	150, 761
HhaI	GCGC	2 cut(s)	158, 739
Hin1I	GRCGYC	1 cut(s)	753
Hin6I	GCGC	2 cut(s)	156, 737
HinP1I	GCGC	2 cut(s)	156, 737
HinfI	GANTC	5 cut(s)	534, 569, 596, 822, 890
HpaII	CCGG	5 cut(s)	357, 531, 777, 797, 894
HphI	GGTGA	4 cut(s)	155, 214, 281, 943
Hpy188I	TCNGA	1 cut(s)	889
Hpy188III	TCNNGA	2 cut(s)	92, 614
Hpy99I	CGWCG	2 cut(s)	166, 755
HpyAV	CCTTC	1 cut(s)	363
HpyCH4III	ACNGT	3 cut(s)	81, 176, 641
HpyCH4IV	ACGT	3 cut(s)	8, 237, 978
HpyCH4V	TGCA	4 cut(s)	324, 331, 590, 759
HpyF10VI	GCNNNNNNNGC	4 cut(s)	302, 311, 314, 402
HpyF3I	CTNAG	3 cut(s)	457, 674, 732
HpySE526I	ACGT	3 cut(s)	8, 237, 978
Hsp92I	GRCGYC	1 cut(s)	753
HspAI	GCGC	2 cut(s)	156, 737
Ksp22I	TGATCA	1 cut(s)	145
Kzo9I	GATC	2 cut(s)	145, 519
LmnI	GCTCC	1 cut(s)	451
Lsp1109I	GCAGC	4 cut(s)	121, 145, 326, 329
LweI	GCATC	2 cut(s)	76, 344
MaeII	ACGT	3 cut(s)	8, 237, 978
MaeIII	GTNAC	5 cut(s)	176, 202, 635, 703, 979
MalI	GATC	2 cut(s)	147, 521
MbiI	CCGCTC	1 cut(s)	222
MboI	GATC	2 cut(s)	145, 519
MboII	GAAGA	3 cut(s)	133, 419, 890
MlsI	TGGCCA	1 cut(s)	626
MluCI	AATT	3 cut(s)	494, 880, 969
MluNI	TGGCCA	1 cut(s)	626
MlyI	GAGTC	2 cut(s)	543, 563
MmeI	TCCRAC	2 cut(s)	231, 473
Mox20I	TGGCCA	1 cut(s)	626
MscI	TGGCCA	1 cut(s)	626
MseI	TTAA	3 cut(s)	12, 243, 972
Msp20I	TGGCCA	1 cut(s)	626
MspI	CCGG	5 cut(s)	357, 531, 777, 797, 894
MspR9I	CCNGG	4 cut(s)	358, 797, 798, 928
MvaI	CCWGG	1 cut(s)	928
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	4 cut(s)	302, 311, 314, 402
NciI	CCSGG	3 cut(s)	358, 797, 798
NdeII	GATC	2 cut(s)	145, 519
NlaIV	GGNNCC	1 cut(s)	529
NmuCI	GTSAC	2 cut(s)	202, 703
NspV	TTCGAA	2 cut(s)	75, 233
PaeR7I	CTCGAG	1 cut(s)	90
PfeI	GAWTC	3 cut(s)	596, 822, 890
PkrI	GCNGC	7 cut(s)	111, 155, 160, 224, 307, 316, 319
PleI	GAGTC	2 cut(s)	542, 563
PpsI	GAGTC	2 cut(s)	542, 563
Ppu21I	YACGTR	1 cut(s)	979
Psp6I	CCWGG	1 cut(s)	926
PspGI	CCWGG	1 cut(s)	926
PspN4I	GGNNCC	1 cut(s)	529
PspPI	GGNCC	1 cut(s)	395
SaqAI	TTAA	3 cut(s)	12, 243, 972
SatI	GCNGC	7 cut(s)	110, 154, 159, 223, 306, 315, 318
Sau3AI	GATC	2 cut(s)	145, 519
Sau96I	GGNCC	1 cut(s)	395
SchI	GAGTC	2 cut(s)	543, 563
ScrFI	CCNGG	4 cut(s)	358, 797, 798, 928
SfaNI	GCATC	2 cut(s)	76, 344
Sfr274I	CTCGAG	1 cut(s)	90
SfuI	TTCGAA	2 cut(s)	75, 233
SlaI	CTCGAG	1 cut(s)	90
SmaI	CCCGGG	1 cut(s)	798
SmlI	CTYRAG	2 cut(s)	90, 743
SmoI	CTYRAG	2 cut(s)	90, 743
SnaBI	TACGTA	1 cut(s)	979
Sse9I	AATT	3 cut(s)	494, 880, 969
SsiI	CCGC	6 cut(s)	154, 200, 222, 225, 305, 858
StyD4I	CCNGG	4 cut(s)	356, 795, 796, 926
StyI	CCWWGG	1 cut(s)	291
TaaI	ACNGT	3 cut(s)	81, 176, 641
TaiI	ACGT	3 cut(s)	11, 240, 981
TasI	AATT	3 cut(s)	494, 880, 969
TauI	GCSGC	3 cut(s)	156, 225, 308
TfiI	GAWTC	3 cut(s)	596, 822, 890
Tru1I	TTAA	3 cut(s)	12, 243, 972
Tru9I	TTAA	3 cut(s)	12, 243, 972
TscAI	CASTG	3 cut(s)	119, 646, 712
TseFI	GTSAC	2 cut(s)	202, 703
TseI	GCWGC	4 cut(s)	109, 158, 314, 317
Tsp45I	GTSAC	2 cut(s)	202, 703
TspDTI	ATGAA	1 cut(s)	780
TspGWI	ACGGA	3 cut(s)	203, 617, 830
TspMI	CCCGGG	1 cut(s)	796
TspRI	CASTG	3 cut(s)	119, 646, 712
XapI	RAATTY	1 cut(s)	494
XhoI	CTCGAG	1 cut(s)	90
XmaI	CCCGGG	1 cut(s)	796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.