Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0009157

Physical Location & Seq

Genomic Coordinates Forward (+)

1441839 .. 1444137

Gene Structure 2299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom111g02420.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGAGGACGTTTAACCTAAAAACCCTCTTGTTATTTGTTCTCATTGCGTTTATTATCTCGTTTTCGAGTTTTTTCAAACTATGTGATGCTCGAGAAGGAGGCAATAAGCAGCACTGGAGGAAGAAACTAAGGGGTTATGGTGGTGATCATCGCCGCGCTGCTTCAAGGGGAGAGAGTTACATCACACCGGAGGAAACTGCGGTGACACTTGGAAAAAAGAGCGGTGGGGGTTCGGACGTGTTTAATGTGTTGGACTATGGTGCTAAAGGTGATGGAAAAGCTGATGACACCAAGGCATTCGAGGCGGCGTGGGCAGCAGCTTGCAAAGTGCAGGCATCAACTATAAGTGTTCCATCCGGGTCTGTATTCCTTGTCAAACCAATCTCTTTCTCTGGCCCTAACTGCGAAGCAAATATCGTGTTTCAGTTAGATGGTAAAATCATAGCTCCAACAAGCTCAGGCGATTGGGGTTCAGGTCTTTTACAATGGATTGAATTCACAAAACTAAAAGGGAGATCGATCAAAGGCACCGGCGTCATTGATGGACAAGGCTCGGTTTGGTGGAATGATTCGCCGAAAGACAATCCTGCTAACGAAACAACGGATAGCAGTCTCGATGATTCGGTAACTAACTGTCCCTCCATATGCATCTCTCGAAATAGTCAAGTAAAGCAGTCCGGCCAAATGCTTTTATTCAGCTTAAAAAAATCATCATGTTCACTTGTGTTAATTTATAGTGAAACTAATCGAGCATGCGTGCTTTTCTCATGTCAACTTCATCAGCAGGCCACAGAAAGTTACAGTGGGAAAATGCCAAGCACCAAACCAACGGCTCTTAGGTTCTATGGAAGCGACCTCGTTAGTGTCACTGGCATAACACTTCAAAACAGCCCTCAGGCGCACCTCAAGTTCGACGCCTGCACAAATGTTCAGGTTTCCGGTATAAGTATTTCATCCCCGGGCAACAGCCCCAACACGGATGGAATCCACCTCCAAAACTCCCAAAATGCCGTCATTTCCGCCAGCAGTATCGCTTGTGAAAGAAAGTTGACACAATATCGACAACAATATAATGAACTGCAACATATAATGACTGCAGGAGATGACTGTGTATCGATACAAACTGGATGCTCGAACGTATACATACACAACGTAAATTGTGGACCTGGACATGGAGTCAGCATTGGAGGGCTAGGGAAGGACAACACGAAAGCTTGCGTTTCGAACGTCACTGTCCGAGACGTTAAAATGCACAGTACACTCACGGGTGTCAGAATAAAGACCTGGCAGGTACGTACTGGAGGATCGGGGTCTGTCCAAAACATAATGTTCTCAAACATTCAACTAACAGAAGTTGAAACGCCGATCATGATTGACCAGTTCTACTGCGACAAGAGCAAGTGCCAAAATCAGAGCTCCGCTGTGGCGATTTCGGGTGTGAATTACGTAAACATACAAGGAACCTACACGTCGAAGGCCGTGCATTTCGCATGCAGTGACAGCTCTCCGTGCACCGGTGTGTCTCTGGATACCATAGAGTTGAAATCAGTTGATGAAGGGAAGCACCTGTACGACCCTTTCTGTTGGCAGGCGTACGGAGTGCTCAAAACAAGCACCGTTCCTCGAATCGACTGCTTGAAGAAGGGGAAGCCGAGCACAATAACTCAATCCGGAAGTAGTGGTTCTTCGTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0009664 GO:0009825 GO:0009827 GO:0009831 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0016798 GO:0030312 GO:0040007 GO:0042545 GO:0042547 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

565

Amino Acids

60.62

Weight (kDa)

8.61

Isoelectric Point (pI)

42.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14650 AT5G14650
fragaria_vesca	FvH4_6g50960 FvH4_6g50960 FvH4_6g50960 FvH4_6g50960 FvH4_6g50960
malus_domestica	MD09G1030100.v1.1 MD09G1030200.v1.1 MD17G1032000.v1.1
prunus_persica	Prupe.3G287200_v2.0.a1
pyrus_communis	pycom111g02420
rosa_chinensis	RchiOBHm_Chr2g0172361
rosa_laevigata	RLG00000022107
rosa_multiflora	Rmu_sc0000467.1_g000009
rosa_roxburghii	Rroxscaffold_2G00079950
rosa_rugosa	Rorug02G0558800
rosa_samantha	Rh2AG633800 Rh2BG647600 Rh2CG614600 Rh2DG662400
rosa_wichuraiana	Rw2G052500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1279
AccB1I	GGYRCC	1 cut(s)	527
AccBSI	CCGCTC	1 cut(s)	222
AccI	GTMKAC	1 cut(s)	1140
AccII	CGCG	1 cut(s)	156
AccIII	TCCGGA	1 cut(s)	1670
AciI	CCGC	6 cut(s)	154, 200, 222, 305, 1020, 1419
AclWI	GGATC	1 cut(s)	1312
AcoI	YGGCCR	1 cut(s)	679
AcsI	RAATTY	1 cut(s)	494
AcyI	GRCGYC	2 cut(s)	534, 915
AfaI	GTAC	5 cut(s)	1258, 1293, 1297, 1571, 1595
AfiI	CCNNNNNNNGG	3 cut(s)	976, 1172, 1514
AflIII	ACRYGT	2 cut(s)	237, 1467
AgeI	ACCGGT	1 cut(s)	1514
AgsI	TTSAA	8 cut(s)	76, 165, 494, 884, 1343, 1358, 1543, 1639
AhdI	GACNNNNNGTC	1 cut(s)	1175
AjiI	CACGTC	2 cut(s)	238, 1470
AjnI	CCWGG	2 cut(s)	1165, 1283
AleI	CACNNNNGTG	1 cut(s)	1517
AluBI	AGCT	8 cut(s)	281, 320, 446, 456, 699, 1214, 1416, 1503
AluI	AGCT	8 cut(s)	281, 320, 446, 456, 699, 1214, 1416, 1503
Alw21I	GWGCWC	4 cut(s)	1418, 1514, 1605, 1658
Alw26I	GTCTC	3 cut(s)	617, 1233, 1527
Alw44I	GTGCAC	1 cut(s)	1510
AlwI	GGATC	1 cut(s)	1312
Ama87I	CYCGRG	2 cut(s)	90, 958
Aor13HI	TCCGGA	1 cut(s)	1670
AoxI	GGCC	4 cut(s)	394, 679, 786, 1476
ApaLI	GTGCAC	1 cut(s)	1510
ApeKI	GCWGC	4 cut(s)	109, 158, 314, 317
ApoI	RAATTY	1 cut(s)	494
AsiGI	ACCGGT	1 cut(s)	1514
AspLEI	GCGC	2 cut(s)	158, 901
AspS9I	GGNCC	2 cut(s)	395, 1163
AsuC2I	CCSGG	3 cut(s)	358, 959, 960
AsuHPI	GGTGA	3 cut(s)	155, 214, 281
AsuII	TTCGAA	1 cut(s)	1223
AvaI	CYCGRG	2 cut(s)	90, 958
AvaII	GGWCC	1 cut(s)	1163
AxyI	CCTNAGG	1 cut(s)	894
BaeGI	GKGCMC	1 cut(s)	1514
BanI	GGYRCC	1 cut(s)	527
BanII	GRGCYC	1 cut(s)	1418
BarI	GAAGNNNNNNTAC	3 cut(s)	1553, 1585, 1669
Bbv12I	GWGCWC	4 cut(s)	1418, 1514, 1605, 1658
BbvI	GCAGC	4 cut(s)	121, 145, 326, 329
BccI	CCATC	5 cut(s)	266, 361, 425, 536, 974
BceAI	ACGGC	3 cut(s)	846, 995, 1463
BcgI	CGANNNNNNTGC	4 cut(s)	1462, 1496, 1614, 1648
BciT130I	CCWGG	2 cut(s)	1167, 1285
BciVI	GTATCC	1 cut(s)	1522
BclI	TGATCA	1 cut(s)	145
BcnI	CCSGG	3 cut(s)	358, 959, 960
BcoDI	GTCTC	3 cut(s)	617, 1233, 1527
BfaI	CTAG	1 cut(s)	1193
BfmI	CTRYAG	1 cut(s)	1095
BfuAI	ACCTGC	1 cut(s)	1279
BfuI	GTATCC	1 cut(s)	1522
BisI	GCNGC	6 cut(s)	110, 154, 159, 306, 315, 318
BlsI	GCNGC	6 cut(s)	111, 155, 160, 307, 316, 319
Bme1390I	CCNGG	5 cut(s)	358, 959, 960, 1167, 1285
Bme18I	GGWCC	1 cut(s)	1163
BmeRI	GACNNNNNGTC	1 cut(s)	1175
BmeT110I	CYCGRG	2 cut(s)	90, 958
BmgBI	CACGTC	2 cut(s)	238, 1470
BmgT120I	GGNCC	2 cut(s)	395, 1163
BmiI	GGNNCC	2 cut(s)	529, 1462
BmrFI	CCNGG	5 cut(s)	358, 959, 960, 1167, 1285
BmsI	GCATC	4 cut(s)	76, 344, 657, 1118
BpmI	CTGGAG	2 cut(s)	136, 1320
Bpu10I	CCTNAGC	1 cut(s)	457
Bpu14I	TTCGAA	1 cut(s)	1223
BpuEI	CTTGAG	1 cut(s)	890
BpuMI	CCSGG	3 cut(s)	358, 959, 960
Bsa29I	ATCGAT	2 cut(s)	518, 1115
BsaAI	YACGTR	2 cut(s)	1295, 1447
BsaHI	GRCGYC	2 cut(s)	534, 915
BsaJI	CCNNGG	3 cut(s)	291, 957, 958
BsaWI	WCCGGW	4 cut(s)	187, 938, 1514, 1670
BsaXI	ACNNNNNCTCC	2 cut(s)	623, 653
Bsc4I	CCNNNNNNNGG	3 cut(s)	976, 1172, 1514
Bse118I	RCCGGY	2 cut(s)	530, 1514
Bse1I	ACTGG	5 cut(s)	119, 874, 1129, 1303, 1378
Bse21I	CCTNAGG	1 cut(s)	894
Bse3DI	GCAATG	1 cut(s)	42
BseAI	TCCGGA	1 cut(s)	1670
BseBI	CCWGG	2 cut(s)	1167, 1285
BseCI	ATCGAT	2 cut(s)	518, 1115
BseDI	CCNNGG	3 cut(s)	291, 957, 958
BseGI	GGATG	4 cut(s)	353, 953, 985, 1133
BseLI	CCNNNNNNNGG	3 cut(s)	976, 1172, 1514
BseMI	GCAATG	1 cut(s)	42
BseMII	CTCAG	2 cut(s)	471, 908
BseNI	ACTGG	5 cut(s)	119, 874, 1129, 1303, 1378
BseSI	GKGCMC	1 cut(s)	1514
BseXI	GCAGC	4 cut(s)	121, 145, 326, 329
BsgI	GTGCAG	2 cut(s)	350, 904
Bsh1236I	CGCG	1 cut(s)	156
BshFI	GGCC	4 cut(s)	396, 681, 788, 1478
BshNI	GGYRCC	1 cut(s)	527
BshTI	ACCGGT	1 cut(s)	1514
BshVI	ATCGAT	2 cut(s)	518, 1115
BsiHKAI	GWGCWC	4 cut(s)	1418, 1514, 1605, 1658
BsiHKCI	CYCGRG	2 cut(s)	90, 958
BsiSI	CCGG	8 cut(s)	188, 357, 531, 678, 939, 959, 1515, 1671
BsiWI	CGTACG	1 cut(s)	1593
BslFI	GGGAC	1 cut(s)	621
BslI	CCNNNNNNNGG	3 cut(s)	976, 1172, 1514
BsmAI	GTCTC	3 cut(s)	617, 1233, 1527
BsmBI	CGTCTC	1 cut(s)	1233
BsmFI	GGGAC	1 cut(s)	621
BsmI	GAATGC	1 cut(s)	296
BsnI	GGCC	4 cut(s)	396, 681, 788, 1478
BsoBI	CYCGRG	2 cut(s)	90, 958
Bsp119I	TTCGAA	1 cut(s)	1223
Bsp1286I	GDGCHC	4 cut(s)	1418, 1514, 1605, 1658
Bsp13I	TCCGGA	1 cut(s)	1670
Bsp143I	GATC	5 cut(s)	145, 515, 519, 1304, 1365
BspACI	CCGC	6 cut(s)	154, 200, 222, 305, 1020, 1419
BspANI	GGCC	4 cut(s)	396, 681, 788, 1478
BspCNI	CTCAG	2 cut(s)	470, 907
BspDI	ATCGAT	2 cut(s)	518, 1115
BspEI	TCCGGA	1 cut(s)	1670
BspFNI	CGCG	1 cut(s)	156
BspHI	TCATGA	1 cut(s)	1368
BspLI	GGNNCC	2 cut(s)	529, 1462
BspMAI	CTGCAG	1 cut(s)	1099
BspMI	ACCTGC	1 cut(s)	1279
BspPI	GGATC	1 cut(s)	1312
BspT104I	TTCGAA	1 cut(s)	1223
BspT107I	GGYRCC	1 cut(s)	527
BsrBI	CCGCTC	1 cut(s)	222
BsrDI	GCAATG	1 cut(s)	42
BsrFI	RCCGGY	2 cut(s)	530, 1514
BsrI	ACTGG	5 cut(s)	119, 874, 1129, 1303, 1378
BssAI	RCCGGY	2 cut(s)	530, 1514
BssECI	CCNNGG	3 cut(s)	291, 957, 958
BssMI	GATC	5 cut(s)	145, 515, 519, 1304, 1365
BssNAI	GTATAC	1 cut(s)	1141
BssNI	GRCGYC	2 cut(s)	534, 915
BssT1I	CCWWGG	1 cut(s)	291
Bst1107I	GTATAC	1 cut(s)	1141
Bst2UI	CCWGG	2 cut(s)	1167, 1285
Bst4CI	ACNGT	6 cut(s)	635, 803, 1109, 1234, 1256, 1618
BstACI	GRCGYC	2 cut(s)	534, 915
BstBAI	YACGTR	2 cut(s)	1295, 1447
BstBI	TTCGAA	1 cut(s)	1223
BstDEI	CTNAG	4 cut(s)	128, 457, 836, 894
BstF5I	GGATG	4 cut(s)	353, 953, 985, 1133
BstFNI	CGCG	1 cut(s)	156
BstHHI	GCGC	2 cut(s)	158, 901
BstKTI	GATC	5 cut(s)	148, 518, 522, 1307, 1368
BstMAI	GTCTC	3 cut(s)	617, 1233, 1527
BstMBI	GATC	5 cut(s)	145, 515, 519, 1304, 1365
BstMWI	GCNNNNNNNGC	7 cut(s)	302, 311, 314, 402, 1395, 1500, 1509
BstNI	CCWGG	2 cut(s)	1167, 1285
BstNSI	RCATGY	2 cut(s)	756, 1494
BstSCI	CCNGG	5 cut(s)	356, 957, 958, 1165, 1283
BstSFI	CTRYAG	1 cut(s)	1095
BstSLI	GKGCMC	1 cut(s)	1514
BstSNI	TACGTA	2 cut(s)	1295, 1447
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	4 cut(s)	121, 145, 326, 329
BstZ17I	GTATAC	1 cut(s)	1141
Bsu15I	ATCGAT	2 cut(s)	518, 1115
Bsu36I	CCTNAGG	1 cut(s)	894
BsuI	GTATCC	1 cut(s)	1522
BsuRI	GGCC	4 cut(s)	396, 681, 788, 1478
BsuTUI	ATCGAT	2 cut(s)	518, 1115
BtgZI	GCGATG	1 cut(s)	134
BtrI	CACGTC	2 cut(s)	238, 1470
BtsCI	GGATG	4 cut(s)	353, 953, 985, 1133
BtsI	GCAGTG	1 cut(s)	1501
BtsIMutI	CAGTG	5 cut(s)	112, 808, 867, 1230, 1501
BveI	ACCTGC	1 cut(s)	1279
CciI	TCATGA	1 cut(s)	1368
CfoI	GCGC	2 cut(s)	158, 901
Cfr10I	RCCGGY	2 cut(s)	530, 1514
Cfr13I	GGNCC	2 cut(s)	395, 1163
Cfr9I	CCCGGG	1 cut(s)	958
ClaI	ATCGAT	2 cut(s)	518, 1115
CseI	GACGC	2 cut(s)	523, 923
Csp6I	GTAC	5 cut(s)	1257, 1292, 1296, 1570, 1594
CspAI	ACCGGT	1 cut(s)	1514
CviAII	CATG	6 cut(s)	714, 753, 768, 1172, 1369, 1491
CviQI	GTAC	5 cut(s)	1257, 1292, 1296, 1570, 1594
DdeI	CTNAG	4 cut(s)	128, 457, 836, 894
DpnI	GATC	5 cut(s)	147, 517, 521, 1306, 1367
DpnII	GATC	5 cut(s)	145, 515, 519, 1304, 1365
DriI	GACNNNNNGTC	1 cut(s)	1175
EaeI	YGGCCR	1 cut(s)	679
Eam1105I	GACNNNNNGTC	1 cut(s)	1175
EciI	GGCGGA	1 cut(s)	1009
Ecl136II	GAGCTC	1 cut(s)	1416
Eco105I	TACGTA	2 cut(s)	1295, 1447
Eco130I	CCWWGG	1 cut(s)	291
Eco24I	GRGCYC	1 cut(s)	1418
Eco47I	GGWCC	1 cut(s)	1163
Eco53kI	GAGCTC	1 cut(s)	1416
Eco81I	CCTNAGG	1 cut(s)	894
Eco88I	CYCGRG	2 cut(s)	90, 958
EcoICRI	GAGCTC	1 cut(s)	1416
EcoRI	GAATTC	1 cut(s)	494
EcoRII	CCWGG	2 cut(s)	1165, 1283
EcoT14I	CCWWGG	1 cut(s)	291
EcoT22I	ATGCAT	1 cut(s)	650
EcoT38I	GRGCYC	1 cut(s)	1418
ErhI	CCWWGG	1 cut(s)	291
Esp3I	CGTCTC	1 cut(s)	1233
FaeI	CATG	6 cut(s)	717, 756, 771, 1175, 1372, 1494
FaqI	GGGAC	1 cut(s)	621
FatI	CATG	6 cut(s)	713, 752, 767, 1171, 1368, 1490
FauNDI	CATATG	1 cut(s)	644
FbaI	TGATCA	1 cut(s)	145
FblI	GTMKAC	1 cut(s)	1140
Fnu4HI	GCNGC	6 cut(s)	110, 154, 159, 306, 315, 318
FokI	GGATG	4 cut(s)	340, 940, 992, 1140
FriOI	GRGCYC	1 cut(s)	1418
Fsp4HI	GCNGC	6 cut(s)	110, 154, 159, 306, 315, 318
FspBI	CTAG	1 cut(s)	1193
GlaI	GCGC	2 cut(s)	157, 900
GluI	GCNGC	6 cut(s)	110, 154, 159, 306, 315, 318
GsuI	CTGGAG	2 cut(s)	136, 1320
HaeIII	GGCC	4 cut(s)	396, 681, 788, 1478
HapII	CCGG	8 cut(s)	188, 357, 531, 678, 939, 959, 1515, 1671
HgaI	GACGC	2 cut(s)	523, 923
HhaI	GCGC	2 cut(s)	158, 901
Hin1I	GRCGYC	2 cut(s)	534, 915
Hin1II	CATG	6 cut(s)	717, 756, 771, 1175, 1372, 1494
Hin6I	GCGC	2 cut(s)	156, 899
HinP1I	GCGC	2 cut(s)	156, 899
HincII	GTYRAC	2 cut(s)	773, 1050
HindII	GTYRAC	2 cut(s)	773, 1050
HindIII	AAGCTT	1 cut(s)	1212
HinfI	GANTC	5 cut(s)	569, 620, 984, 1176, 1626
HpaII	CCGG	8 cut(s)	188, 357, 531, 678, 939, 959, 1515, 1671
HphI	GGTGA	3 cut(s)	155, 214, 281
Hpy166II	GTNNAC	8 cut(s)	719, 773, 1050, 1141, 1163, 1259, 1450, 1512
Hpy188I	TCNGA	4 cut(s)	235, 1238, 1274, 1413
Hpy188III	TCNNGA	6 cut(s)	92, 614, 654, 1369, 1526, 1671
Hpy8I	GTNNAC	8 cut(s)	719, 773, 1050, 1141, 1163, 1259, 1450, 1512
Hpy99I	CGWCG	2 cut(s)	917, 1474
HpyAV	CCTTC	5 cut(s)	89, 1192, 1468, 1550, 1636
HpyCH4III	ACNGT	6 cut(s)	635, 803, 1109, 1234, 1256, 1618
HpyCH4IV	ACGT	9 cut(s)	8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
HpyF10VI	GCNNNNNNNGC	7 cut(s)	302, 311, 314, 402, 1395, 1500, 1509
HpyF3I	CTNAG	4 cut(s)	128, 457, 836, 894
HpySE526I	ACGT	9 cut(s)	8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
Hsp92I	GRCGYC	2 cut(s)	534, 915
Hsp92II	CATG	6 cut(s)	717, 756, 771, 1175, 1372, 1494
HspAI	GCGC	2 cut(s)	156, 899
Kpn2I	TCCGGA	1 cut(s)	1670
Ksp22I	TGATCA	1 cut(s)	145
Kzo9I	GATC	5 cut(s)	145, 515, 519, 1304, 1365
LmnI	GCTCC	2 cut(s)	451, 1421
Lsp1109I	GCAGC	4 cut(s)	121, 145, 326, 329
LweI	GCATC	4 cut(s)	76, 344, 657, 1118
MaeI	CTAG	1 cut(s)	1193
MaeII	ACGT	9 cut(s)	8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
MaeIII	GTNAC	7 cut(s)	176, 202, 625, 797, 865, 1228, 1496
MalI	GATC	5 cut(s)	147, 517, 521, 1306, 1367
MbiI	CCGCTC	1 cut(s)	222
MboI	GATC	5 cut(s)	145, 515, 519, 1304, 1365
MboII	GAAGA	3 cut(s)	133, 1651, 1677
MhlI	GDGCHC	4 cut(s)	1418, 1514, 1605, 1658
MluCI	AATT	4 cut(s)	494, 729, 1156, 1441
MlyI	GAGTC	1 cut(s)	1185
MmeI	TCCRAC	2 cut(s)	231, 473
Mph1103I	ATGCAT	1 cut(s)	650
MroI	TCCGGA	1 cut(s)	1670
MseI	TTAA	5 cut(s)	12, 243, 701, 728, 1245
MslI	CAYNNNNRTG	1 cut(s)	1517
MspA1I	CMGCKG	1 cut(s)	1421
MspI	CCGG	8 cut(s)	188, 357, 531, 678, 939, 959, 1515, 1671
MspR9I	CCNGG	5 cut(s)	358, 959, 960, 1167, 1285
Mva1269I	GAATGC	1 cut(s)	296
MvaI	CCWGG	2 cut(s)	1167, 1285
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	7 cut(s)	302, 311, 314, 402, 1395, 1500, 1509
NciI	CCSGG	3 cut(s)	358, 959, 960
NdeI	CATATG	1 cut(s)	644
NdeII	GATC	5 cut(s)	145, 515, 519, 1304, 1365
NlaIII	CATG	6 cut(s)	717, 756, 771, 1175, 1372, 1494
NlaIV	GGNNCC	2 cut(s)	529, 1462
NmeAIII	GCCGAG	1 cut(s)	1677
NmuCI	GTSAC	4 cut(s)	202, 865, 1228, 1496
NsiI	ATGCAT	1 cut(s)	650
NspI	RCATGY	2 cut(s)	756, 1494
NspV	TTCGAA	1 cut(s)	1223
OliI	CACNNNNGTG	1 cut(s)	1517
PaeI	GCATGC	2 cut(s)	756, 1494
PaeR7I	CTCGAG	1 cut(s)	90
PagI	TCATGA	1 cut(s)	1368
PctI	GAATGC	1 cut(s)	296
PfeI	GAWTC	4 cut(s)	569, 620, 984, 1626
Pfl23II	CGTACG	1 cut(s)	1593
PinAI	ACCGGT	1 cut(s)	1514
PkrI	GCNGC	6 cut(s)	111, 155, 160, 307, 316, 319
PleI	GAGTC	1 cut(s)	1184
PpsI	GAGTC	1 cut(s)	1184
Ppu21I	YACGTR	2 cut(s)	1295, 1447
Psp124BI	GAGCTC	1 cut(s)	1418
Psp6I	CCWGG	2 cut(s)	1165, 1283
PspGI	CCWGG	2 cut(s)	1165, 1283
PspLI	CGTACG	1 cut(s)	1593
PspN4I	GGNNCC	2 cut(s)	529, 1462
PspPI	GGNCC	2 cut(s)	395, 1163
PstI	CTGCAG	1 cut(s)	1099
RsaI	GTAC	5 cut(s)	1258, 1293, 1297, 1571, 1595
RsaNI	GTAC	5 cut(s)	1257, 1292, 1296, 1570, 1594
RseI	CAYNNNNRTG	1 cut(s)	1517
SacI	GAGCTC	1 cut(s)	1418
SaqAI	TTAA	5 cut(s)	12, 243, 701, 728, 1245
SatI	GCNGC	6 cut(s)	110, 154, 159, 306, 315, 318
Sau3AI	GATC	5 cut(s)	145, 515, 519, 1304, 1365
Sau96I	GGNCC	2 cut(s)	395, 1163
SchI	GAGTC	1 cut(s)	1185
ScrFI	CCNGG	5 cut(s)	358, 959, 960, 1167, 1285
SduI	GDGCHC	4 cut(s)	1418, 1514, 1605, 1658
SfaNI	GCATC	4 cut(s)	76, 344, 657, 1118
SfcI	CTRYAG	1 cut(s)	1095
Sfr274I	CTCGAG	1 cut(s)	90
SfuI	TTCGAA	1 cut(s)	1223
SgrAI	CRCCGGYG	2 cut(s)	530, 1514
SinI	GGWCC	1 cut(s)	1163
SlaI	CTCGAG	1 cut(s)	90
SmaI	CCCGGG	1 cut(s)	960
SmiMI	CAYNNNNRTG	1 cut(s)	1517
SmlI	CTYRAG	2 cut(s)	90, 905
SmoI	CTYRAG	2 cut(s)	90, 905
SnaBI	TACGTA	2 cut(s)	1295, 1447
SphI	GCATGC	2 cut(s)	756, 1494
Sse9I	AATT	4 cut(s)	494, 729, 1156, 1441
SsiI	CCGC	6 cut(s)	154, 200, 222, 305, 1020, 1419
SspMI	CTAG	1 cut(s)	1193
SstI	GAGCTC	1 cut(s)	1418
StyD4I	CCNGG	5 cut(s)	356, 957, 958, 1165, 1283
StyI	CCWWGG	1 cut(s)	291
TaaI	ACNGT	6 cut(s)	635, 803, 1109, 1234, 1256, 1618
TaiI	ACGT	9 cut(s)	11, 240, 1140, 1155, 1230, 1245, 1297, 1449, 1472
TasI	AATT	4 cut(s)	494, 729, 1156, 1441
TatI	WGTACW	1 cut(s)	1256
TauI	GCSGC	2 cut(s)	156, 308
TfiI	GAWTC	4 cut(s)	569, 620, 984, 1626
Tru1I	TTAA	5 cut(s)	12, 243, 701, 728, 1245
Tru9I	TTAA	5 cut(s)	12, 243, 701, 728, 1245
TscAI	CASTG	5 cut(s)	119, 808, 874, 1237, 1501
TseFI	GTSAC	4 cut(s)	202, 865, 1228, 1496
TseI	GCWGC	4 cut(s)	109, 158, 314, 317
Tsp45I	GTSAC	4 cut(s)	202, 865, 1228, 1496
TspDTI	ATGAA	4 cut(s)	767, 942, 1089, 1569
TspGWI	ACGGA	4 cut(s)	617, 992, 1497, 1611
TspMI	CCCGGG	1 cut(s)	958
TspRI	CASTG	5 cut(s)	119, 808, 874, 1237, 1501
VneI	GTGCAC	1 cut(s)	1510
VpaK11BI	GGWCC	1 cut(s)	1163
XapI	RAATTY	1 cut(s)	494
XceI	RCATGY	2 cut(s)	756, 1494
XhoI	CTCGAG	1 cut(s)	90
XmaI	CCCGGG	1 cut(s)	958
XmiI	GTMKAC	1 cut(s)	1140
XspI	CTAG	1 cut(s)	1193
Zsp2I	ATGCAT	1 cut(s)	650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.