AT5G15450

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009881
Physical Location & Seq
Reverse (-)
5014212 .. 5018347
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15450.1

Sequence Viewer

Length: 2907 bp
ATGGCGACGGCTACGACGACTGCTACGGCGGCGTTTAGTGGTGTAGTCAGTGTAGGAACGGAGACTCGAAGGATTTATTCGTTTTCTCATCTTCAACCTTCTGCGGCTTTTCCGGCGAAGCCTAGTTCCTTCAAATCTCTCAAATTAAAGCAGAGCGCGAGGCTCACACGGCGGCTTGATCATCGGCCGTTCGTTGTCCGATGTGAAGCTTCTTCTTCTAATGGAAGGCTTACACAGCAAGAATTCACAGAAATGGCGTGGCAATCGATAGTTTCGTCACCAGATGTTGCAAAAGAGAATAAGCAACAGATTGTGGAGACAGAGCATCTAATGAAAGCTCTTTTGGAGCAGAAGAATGGTTTAGCTCGTAGGATTTTCTCTAAGATCGGTGTGGATAACACAAAGGTTCTAGAAGCAACAGAGAAGTTCATTCAACGCCAACCAAAGGTGTATGGAGATGCTGCTGGTTCGATGTTGGGGCGTGATTTGGAAGCTCTGTTCCAGAGAGCAAGACAGTTTAAGAAAGATTTAAAGGACTCTTATGTGTCAGTGGAGCATTTGGTTCTTGCTTTTGCTGATGATAAGCGGTTTGGGAAACAGTTGTTTAAGGATTTTCAGATATCAGAGAGGAGCTTGAAATCTGCAATTGAATCCATTAGAGGGAAACAATCAGTCATTGACCAAGATCCGGAAGGCAAGTATGAGGCGTTGGAGAAGTATGGAAAAGACTTGACAGCAATGGCAAGAGAGGGAAAACTAGATCCTGTGATAGGTAGAGATGACGAGATCCGAAGATGCATTCAGATTCTCTCAAGGAGAACAAAGAACAACCCTGTGTTGATCGGTGAACCAGGTGTTGGTAAAACTGCAATTTCAGAAGGACTTGCTCAGAGGATTGTGCAAGGAGATGTACCTCAAGCATTGATGAACAGAAAGTTGATATCTCTTGATATGGGTGCTCTCATTGCTGGAGCAAAGTATCGGGGAGAATTCGAAGACAGACTAAAAGCTGTGCTTAAGGAAGTCACAGACTCAGAAGGCCAAATCATTTTGTTCATCGATGAGATCCATACAGTTGTTGGTGCAGGTGCGACTAATGGGGCCATGGATGCTGGTAATCTGCTGAAACCGATGCTGGGCCGAGGCGAGTTACGTTGTATCGGTGCAACCACGCTTGATGAATATAGGAAATACATAGAGAAAGATCCAGCCTTGGAGCGTAGGTTTCAACAGGTTTATGTTGATCAACCTACGGTTGAAGACACGATTTCTATTCTCCGTGGTTTGCGGGAAAGGTATGAACTACATCATGGAGTTCGCATCTCAGATAGTGCTCTAGTGGAAGCTGCTATCCTCTCAGACCGTTACATCAGTGGCCGGTTTCTACCCGATAAAGCAATTGACTTGGTCGATGAAGCGGCAGCCAAATTGAAAATGGAAATCACTTCGAAACCTACAGCTCTTGACGAGCTTGACCGTTCGGTAATAAAGCTTGAAATGGAGAGGCTTTCACTTACCAATGATACAGACAAAGCTTCTAGAGAGAGATTGAATCGAATTGAAACTGAGTTAGTGCTATTAAAGGAGAAACAAGCTGAGTTAACTGAACAGTGGGAGCACGAGAGGTCTGTTATGTCCCGTCTTCAGTCGATTAAAGAGGAGATTGATCGAGTAAACCTCGAGATCCAGCAGGCGGAACGTGAATACGATCTCAACCGTGCAGCTGAGCTCAAATACGGAAGCTTAAACTCATTGCAGCGACAGCTTAATGAAGCAGAGAAAGAGCTGAACGAGTACTTAAGCTCTGGGAAATCTATGTTCAGAGAAGAGGTTTTGGGTAGCGATATCGCCGAGATTGTGAGTAAATGGACTGGAATCCCGGTATCAAAACTTCAACAGTCTGAACGAGATAAGCTTCTACATTTGGAAGAAGAATTGCATAAAAGAGTAGTCGGTCAAAACCCAGCAGTGACTGCGGTAGCTGAAGCAATCCAACGATCAAGAGCTGGTCTTTCGGATCCAGGCCGTCCTATAGCTAGTTTCATGTTTATGGGACCAACTGGTGTTGGTAAAACAGAGCTAGCCAAAGCACTTGCTTCTTATATGTTCAACACTGAAGAAGCTTTAGTGAGAATTGACATGAGTGAATACATGGAGAAGCATGCGGTGTCTAGGCTCATTGGAGCTCCACCTGGATATGTTGGTTACGAAGAAGGAGGACAGTTAACAGAAACGGTTAGAAGAAGGCCTTATTCGGTCATTCTATTTGATGAGATTGAGAAAGCTCATGGAGATGTCTTCAATGTGTTTCTCCAAATCTTGGACGATGGGCGAGTCACAGATTCTCAAGGACGTACAGTGAGTTTCACCAATACCGTCATTATTATGACCTCAAATGTTGGTTCGCAGTTTATTCTGAACAACACAGATGATGATGCTAATGAGCTCTCTTATGAAACGATTAAAGAGAGAGTGATGAATGCTGCAAGGTCAATCTTTCGTCCTGAATTCATGAATAGGGTTGATGAGTATATAGTTTTCAAACCTCTTGACCGCGAGCAGATCAACCGCATTGTCCGGTTACAGCTGGCACGTGTGCAGAAGCGAATTGCAGACAGGAAAATGAAGATAAACATCACGGATGCAGCAGTCGATCTTCTTGGAAGCCTAGGGTATGATCCAAACTATGGAGCTAGACCTGTTAAGCGTGTGATACAGCAAAACATTGAGAATGAGCTTGCTAAGGGTATCTTGAGAGGAGATTTCAAGGAGGAAGATGGCATTTTGATCGATACCGAGGTCACAGCGTTCTCCAATGGCCAGTTACCTCAGCAAAAGCTCACATTCAAGAAGATTGAGTCAGAAACAGCAGATGCTGAACAAGAAGAAGCAGCTTTCTCAAAATAG

Protein Analysis

968

Amino Acids

108.94

Weight (kDa)

5.93

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 81 - 203 4e-35 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 261 - 362 7.2e-06 SMAX1 nucleotide binding domain
AAA PF00004 279 - 410 3.9e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 418 - 519 5.3e-34 AAA lid domain
Sigma54_activat PF00158 673 - 799 8.4e-06 Sigma-54 interaction domain
AAA_2 PF07724 676 - 843 7.9e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 681 - 804 7.7e-11 AAA domain (dynein-related subfamily)
AAA PF00004 682 - 799 3.7e-09 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 850 - 928 1e-26 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1076
Acc36I ACCTGC 1 cut(s) 1076
AccB7I CCANNNNNTGG 3 cut(s) 857, 2320, 2687
AccII CGCG 2 cut(s) 158, 2556
AccIII TCCGGA 1 cut(s) 688
AclWI GGATC 9 cut(s) 680, 755, 781, 1060, 1199, 1678, 2012, 2025, 2672
AcoI YGGCCR 3 cut(s) 185, 1375, 2818
AcsI RAATTY 3 cut(s) 242, 989, 2507
AcuI CTGAAG 3 cut(s) 1628, 2004, 2136
AcvI CACGTG 1 cut(s) 2594
AfaI GTAC 3 cut(s) 912, 1796, 2356
AfiI CCNNNNNNNGG 9 cut(s) 445, 660, 688, 770, 857, 1136, 1693, 2320, 2687
AflII CTTAAG 2 cut(s) 1016, 1798
AflIII ACRYGT 1 cut(s) 2593
AjnI CCWGG 3 cut(s) 850, 2020, 2191
AjuI GAANNNNNNNTTGG 4 cut(s) 326, 358, 840, 872
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
Alw21I GWGCWC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
Alw26I GTCTC 2 cut(s) 56, 311
AlwI GGATC 9 cut(s) 680, 755, 781, 1060, 1199, 1678, 2012, 2025, 2672
AlwNI CAGNNNCTG 2 cut(s) 1973, 2876
Ama87I CYCGRG 1 cut(s) 1679
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 8 cut(s) 185, 1039, 1101, 1138, 1375, 2023, 2246, 2818
ApeKI GCWGC 8 cut(s) 461, 1346, 1421, 1721, 1756, 2483, 2645, 2891
ApoI RAATTY 3 cut(s) 242, 989, 2507
AspA2I CCTAGG 1 cut(s) 2668
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 1101, 1138, 2054
AsuC2I CCSGG 1 cut(s) 1880
AsuHPI GGTGA 3 cut(s) 270, 857, 2359
AsuII TTCGAA 2 cut(s) 993, 1448
AsuNHI GCTAGC 1 cut(s) 2080
AvaI CYCGRG 1 cut(s) 1679
AvaII GGWCC 1 cut(s) 2054
AvrII CCTAGG 1 cut(s) 2668
BaeI ACNNNNGTAYC 2 cut(s) 2784, 2817
BalI TGGCCA 1 cut(s) 2820
BamHI GGATCC 1 cut(s) 2017
BanII GRGCYC 3 cut(s) 1731, 2188, 2448
BarI GAAGNNNNNNTAC 2 cut(s) 1875, 1907
BauI CACGAG 1 cut(s) 1619
BbrPI CACGTG 1 cut(s) 2594
BbsI GAAGAC 4 cut(s) 1002, 1266, 1634, 2290
Bbv12I GWGCWC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
BbvCI CCTCAGC 1 cut(s) 2829
BbvI GCAGC 8 cut(s) 448, 1333, 1433, 1733, 1768, 2470, 2657, 2903
BccI CCATC 2 cut(s) 2321, 2771
BceAI ACGGC 5 cut(s) 24, 42, 172, 185, 2010
BcgI CGANNNNNNTGC 2 cut(s) 2770, 2804
BciT130I CCWGG 3 cut(s) 852, 2022, 2193
BclI TGATCA 2 cut(s) 178, 1243
BcnI CCSGG 1 cut(s) 1880
BcoDI GTCTC 2 cut(s) 56, 311
BfmI CTRYAG 2 cut(s) 1455, 2031
BfrI CTTAAG 2 cut(s) 1016, 1798
BfuAI ACCTGC 1 cut(s) 1076
BlnI CCTAGG 1 cut(s) 2668
BlpI GCTNAGC 1 cut(s) 1725
BmcAI AGTACT 1 cut(s) 1796
Bme1390I CCNGG 4 cut(s) 852, 1880, 2022, 2193
Bme18I GGWCC 1 cut(s) 2054
BmeT110I CYCGRG 1 cut(s) 1679
BmgT120I GGNCC 3 cut(s) 1101, 1138, 2054
BmiI GGNNCC 3 cut(s) 1102, 2019, 2055
BmrFI CCNGG 4 cut(s) 852, 1880, 2022, 2193
BmsI GCATC 9 cut(s) 334, 448, 785, 1099, 1122, 1329, 2425, 2632, 2863
BmtI GCTAGC 1 cut(s) 2084
BpiI GAAGAC 4 cut(s) 1002, 1266, 1634, 2290
BplI GAGNNNNNCTC 2 cut(s) 1662, 1694
BpmI CTGGAG 1 cut(s) 990
Bpu10I CCTNAGC 2 cut(s) 2742, 2829
Bpu1102I GCTNAGC 1 cut(s) 1725
Bpu14I TTCGAA 2 cut(s) 993, 1448
BpuEI CTTGAG 4 cut(s) 796, 900, 2331, 2773
BpuMI CCSGG 1 cut(s) 1880
Bsa29I ATCGAT 3 cut(s) 266, 1059, 2790
BsaAI YACGTR 1 cut(s) 2594
BsaBI GATNNNNATC 1 cut(s) 2633
BsaJI CCNNGG 6 cut(s) 1104, 1141, 1212, 1279, 2668, 2796
BsaWI WCCGGW 2 cut(s) 688, 2577
BsaXI ACNNNNNCTCC 2 cut(s) 1208, 1238
Bsc4I CCNNNNNNNGG 9 cut(s) 445, 660, 688, 770, 857, 1136, 1693, 2320, 2687
Bse118I RCCGGY 1 cut(s) 1377
Bse1I ACTGG 3 cut(s) 1876, 2065, 2821
Bse3DI GCAATG 3 cut(s) 744, 963, 1751
Bse8I GATNNNNATC 1 cut(s) 2633
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 3 cut(s) 852, 2022, 2193
BseCI ATCGAT 3 cut(s) 266, 1059, 2790
BseDI CCNNGG 6 cut(s) 1104, 1141, 1212, 1279, 2668, 2796
BseGI GGATG 2 cut(s) 1114, 2647
BseJI GATNNNNATC 1 cut(s) 2633
BseLI CCNNNNNNNGG 9 cut(s) 445, 660, 688, 770, 857, 1136, 1693, 2320, 2687
BseMI GCAATG 3 cut(s) 744, 963, 1751
BseMII CTCAG 8 cut(s) 902, 1047, 1338, 1371, 1557, 1587, 1716, 2843
BseNI ACTGG 3 cut(s) 1876, 2065, 2821
BseRI GAGGAG 3 cut(s) 643, 1673, 2772
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 8 cut(s) 448, 1333, 1433, 1733, 1768, 2470, 2657, 2903
BseYI CCCAGC 2 cut(s) 1135, 1963
BsgI GTGCAG 3 cut(s) 1104, 1740, 2618
Bsh1236I CGCG 2 cut(s) 158, 2556
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 8 cut(s) 187, 1041, 1103, 1140, 1377, 2025, 2248, 2820
BshVI ATCGAT 3 cut(s) 266, 1059, 2790
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
BsiHKCI CYCGRG 1 cut(s) 1679
BsiSI CCGG 5 cut(s) 113, 689, 1378, 1880, 2578
BslFI GGGAC 2 cut(s) 1621, 2067
BslI CCNNNNNNNGG 9 cut(s) 445, 660, 688, 770, 857, 1136, 1693, 2320, 2687
BsmAI GTCTC 2 cut(s) 56, 311
BsmFI GGGAC 2 cut(s) 1621, 2067
BsmI GAATGC 2 cut(s) 798, 2485
BsnI GGCC 8 cut(s) 187, 1041, 1103, 1140, 1377, 2025, 2248, 2820
BsoBI CYCGRG 1 cut(s) 1679
Bsp119I TTCGAA 2 cut(s) 993, 1448
Bsp1286I GDGCHC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
Bsp13I TCCGGA 1 cut(s) 688
Bsp1720I GCTNAGC 1 cut(s) 1725
Bsp19I CCATGG 1 cut(s) 1104
BspANI GGCC 8 cut(s) 187, 1041, 1103, 1140, 1377, 2025, 2248, 2820
BspCNI CTCAG 8 cut(s) 901, 1046, 1337, 1370, 1558, 1588, 1717, 2842
BspDI ATCGAT 3 cut(s) 266, 1059, 2790
BspEI TCCGGA 1 cut(s) 688
BspFNI CGCG 2 cut(s) 158, 2556
BspHI TCATGA 1 cut(s) 2511
BspLI GGNNCC 3 cut(s) 1102, 2019, 2055
BspMI ACCTGC 1 cut(s) 1076
BspOI GCTAGC 1 cut(s) 2084
BspPI GGATC 9 cut(s) 680, 755, 781, 1060, 1199, 1678, 2012, 2025, 2672
BspT104I TTCGAA 2 cut(s) 993, 1448
BspTI CTTAAG 2 cut(s) 1016, 1798
BsrDI GCAATG 3 cut(s) 744, 963, 1751
BsrFI RCCGGY 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 1876, 2065, 2821
BssAI RCCGGY 1 cut(s) 1377
BssECI CCNNGG 6 cut(s) 1104, 1141, 1212, 1279, 2668, 2796
BssSI CACGAG 1 cut(s) 1619
BssT1I CCWWGG 3 cut(s) 1104, 1212, 2668
Bst2BI CACGAG 1 cut(s) 1619
Bst2UI CCWGG 3 cut(s) 852, 2022, 2193
Bst6I CTCTTC 1 cut(s) 1821
BstAFI CTTAAG 2 cut(s) 1016, 1798
BstAPI GCANNNNNTGC 1 cut(s) 1973
BstBAI YACGTR 1 cut(s) 2594
BstBI TTCGAA 2 cut(s) 993, 1448
BstC8I GCNNGC 6 cut(s) 1692, 2082, 2163, 2558, 2589, 2739
BstDSI CCRYGG 2 cut(s) 1104, 1279
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 2 cut(s) 1114, 2647
BstFNI CGCG 2 cut(s) 158, 2556
BstHHI GCGC 1 cut(s) 158
BstMAI GTCTC 2 cut(s) 56, 311
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 8 cut(s) 29, 113, 169, 235, 965, 1109, 1762, 1973
BstNI CCWGG 3 cut(s) 852, 2022, 2193
BstNSI RCATGY 1 cut(s) 2165
BstSCI CCNGG 4 cut(s) 850, 1878, 2020, 2191
BstSFI CTRYAG 2 cut(s) 1455, 2031
BstUI CGCG 2 cut(s) 158, 2556
BstV1I GCAGC 8 cut(s) 448, 1333, 1433, 1733, 1768, 2470, 2657, 2903
BstV2I GAAGAC 4 cut(s) 1002, 1266, 1634, 2290
BstX2I RGATCY 7 cut(s) 685, 760, 786, 1065, 1204, 1683, 2017
BstYI RGATCY 7 cut(s) 685, 760, 786, 1065, 1204, 1683, 2017
BstZI CGGCCG 1 cut(s) 185
Bsu15I ATCGAT 3 cut(s) 266, 1059, 2790
BsuRI GGCC 8 cut(s) 187, 1041, 1103, 1140, 1377, 2025, 2248, 2820
BsuTUI ATCGAT 3 cut(s) 266, 1059, 2790
BtgI CCRYGG 2 cut(s) 1104, 1279
BtsCI GGATG 2 cut(s) 1114, 2647
BtsI GCAGTG 1 cut(s) 1974
BtsIMutI CAGTG 7 cut(s) 55, 555, 1378, 1616, 1974, 2112, 2364
BveI ACCTGC 1 cut(s) 1076
Cac8I GCNNGC 6 cut(s) 1692, 2082, 2163, 2558, 2589, 2739
CaiI CAGNNNCTG 2 cut(s) 1973, 2876
CciI TCATGA 1 cut(s) 2511
CfoI GCGC 1 cut(s) 158
Cfr10I RCCGGY 1 cut(s) 1377
Cfr13I GGNCC 3 cut(s) 1101, 1138, 2054
ClaI ATCGAT 3 cut(s) 266, 1059, 2790
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 3 cut(s) 911, 1795, 2355
CviAII CATG 8 cut(s) 1105, 1310, 2044, 2140, 2152, 2162, 2288, 2512
CviQI GTAC 3 cut(s) 911, 1795, 2355
DraI TTTAAA 1 cut(s) 531
EaeI YGGCCR 3 cut(s) 185, 1375, 2818
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 1821
EarI CTCTTC 1 cut(s) 1821
EciI GGCGGA 1 cut(s) 1709
Ecl136II GAGCTC 3 cut(s) 1729, 2186, 2446
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 3 cut(s) 1104, 1212, 2668
Eco147I AGGCCT 1 cut(s) 2248
Eco24I GRGCYC 3 cut(s) 1731, 2188, 2448
Eco32I GATATC 3 cut(s) 621, 942, 1846
Eco47I GGWCC 1 cut(s) 2054
Eco52I CGGCCG 1 cut(s) 185
Eco53kI GAGCTC 3 cut(s) 1729, 2186, 2446
Eco57I CTGAAG 3 cut(s) 1628, 2004, 2136
Eco72I CACGTG 1 cut(s) 2594
Eco88I CYCGRG 1 cut(s) 1679
EcoICRI GAGCTC 3 cut(s) 1729, 2186, 2446
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRI GAATTC 3 cut(s) 242, 989, 2507
EcoRII CCWGG 3 cut(s) 850, 2020, 2191
EcoRV GATATC 3 cut(s) 621, 942, 1846
EcoT14I CCWWGG 3 cut(s) 1104, 1212, 2668
EcoT22I ATGCAT 1 cut(s) 800
EcoT38I GRGCYC 3 cut(s) 1731, 2188, 2448
ErhI CCWWGG 3 cut(s) 1104, 1212, 2668
FaeI CATG 8 cut(s) 1108, 1313, 2047, 2143, 2155, 2165, 2291, 2515
FalI AAGNNNNNCTT 6 cut(s) 999, 1031, 2233, 2265, 2735, 2767
FaqI GGGAC 2 cut(s) 1621, 2067
FatI CATG 8 cut(s) 1104, 1309, 2043, 2139, 2151, 2161, 2287, 2511
FauI CCCGC 1 cut(s) 1281
FbaI TGATCA 2 cut(s) 178, 1243
FokI GGATG 2 cut(s) 1121, 2654
FriOI GRGCYC 3 cut(s) 1731, 2188, 2448
GlaI GCGC 1 cut(s) 157
GsaI CCCAGC 2 cut(s) 1139, 1967
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 8 cut(s) 187, 1041, 1103, 1140, 1377, 2025, 2248, 2820
HapII CCGG 5 cut(s) 113, 689, 1378, 1880, 2578
HhaI GCGC 1 cut(s) 158
Hin1II CATG 8 cut(s) 1108, 1313, 2047, 2143, 2155, 2165, 2291, 2515
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 2 cut(s) 1602, 2226
HindII GTYRAC 2 cut(s) 1602, 2226
HindIII AAGCTT 6 cut(s) 207, 1490, 1533, 1741, 1913, 2121
HpaI GTTAAC 2 cut(s) 1602, 2226
HpaII CCGG 5 cut(s) 113, 689, 1378, 1880, 2578
HphI GGTGA 3 cut(s) 270, 857, 2359
Hpy166II GTNNAC 4 cut(s) 848, 1602, 1675, 2226
Hpy8I GTNNAC 4 cut(s) 848, 1602, 1675, 2226
Hpy99I CGWCG 2 cut(s) 10, 19
HpyAV CCTTC 9 cut(s) 63, 108, 139, 219, 686, 872, 1031, 2207, 2238
HpyCH4IV ACGT 4 cut(s) 1153, 1699, 2353, 2593
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 113, 169, 235, 965, 1109, 1762, 1973
HpySE526I ACGT 4 cut(s) 1153, 1699, 2353, 2593
Hsp92II CATG 8 cut(s) 1108, 1313, 2047, 2143, 2155, 2165, 2291, 2515
HspAI GCGC 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 688
Ksp22I TGATCA 2 cut(s) 178, 1243
KspAI GTTAAC 2 cut(s) 1602, 2226
LmnI GCTCC 9 cut(s) 346, 553, 630, 971, 1216, 1615, 2183, 2191, 2690
Lsp1109I GCAGC 8 cut(s) 448, 1333, 1433, 1733, 1768, 2470, 2657, 2903
LweI GCATC 9 cut(s) 334, 448, 785, 1099, 1122, 1329, 2425, 2632, 2863
MabI ACCWGGT 1 cut(s) 850
MaeII ACGT 4 cut(s) 1153, 1699, 2353, 2593
MfeI CAATTG 2 cut(s) 645, 1398
MflI RGATCY 7 cut(s) 685, 760, 786, 1065, 1204, 1683, 2017
MhlI GDGCHC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
MlsI TGGCCA 1 cut(s) 2820
MluNI TGGCCA 1 cut(s) 2820
MlyI GAGTC 5 cut(s) 58, 530, 1025, 2343, 2867
MmeI TCCRAC 2 cut(s) 690, 2017
Mox20I TGGCCA 1 cut(s) 2820
Mph1103I ATGCAT 1 cut(s) 800
MroI TCCGGA 1 cut(s) 688
MscI TGGCCA 1 cut(s) 2820
MslI CAYNNNNRTG 4 cut(s) 251, 2048, 2292, 2384
Msp20I TGGCCA 1 cut(s) 2820
MspA1I CMGCKG 2 cut(s) 1724, 2587
MspCI CTTAAG 2 cut(s) 1016, 1798
MspI CCGG 5 cut(s) 113, 689, 1378, 1880, 2578
MspR9I CCNGG 4 cut(s) 852, 1880, 2022, 2193
MunI CAATTG 2 cut(s) 645, 1398
Mva1269I GAATGC 2 cut(s) 798, 2485
MvaI CCWGG 3 cut(s) 852, 2022, 2193
MvnI CGCG 2 cut(s) 158, 2556
MwoI GCNNNNNNNGC 8 cut(s) 29, 113, 169, 235, 965, 1109, 1762, 1973
NciI CCSGG 1 cut(s) 1880
NcoI CCATGG 1 cut(s) 1104
NheI GCTAGC 1 cut(s) 2080
NlaIII CATG 8 cut(s) 1108, 1313, 2047, 2143, 2155, 2165, 2291, 2515
NlaIV GGNNCC 3 cut(s) 1102, 2019, 2055
NmeAIII GCCGAG 2 cut(s) 1166, 1876
NmuCI GTSAC 5 cut(s) 276, 1024, 1969, 2335, 2800
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 1 cut(s) 2165
NspV TTCGAA 2 cut(s) 993, 1448
PaeI GCATGC 1 cut(s) 2165
PaeR7I CTCGAG 1 cut(s) 1679
PagI TCATGA 1 cut(s) 2511
PaqCI CACCTGC 1 cut(s) 1076
PceI AGGCCT 1 cut(s) 2248
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 272
PctI GAATGC 2 cut(s) 798, 2485
PfeI GAWTC 5 cut(s) 650, 805, 1552, 1875, 2342
PflFI GACNNNGTC 1 cut(s) 1406
PflMI CCANNNNNTGG 3 cut(s) 857, 2320, 2687
PleI GAGTC 5 cut(s) 58, 530, 1025, 2342, 2866
PmaCI CACGTG 1 cut(s) 2594
PmlI CACGTG 1 cut(s) 2594
PpsI GAGTC 5 cut(s) 58, 530, 1025, 2342, 2866
Ppu21I YACGTR 1 cut(s) 2594
Psp124BI GAGCTC 3 cut(s) 1731, 2188, 2448
Psp6I CCWGG 3 cut(s) 850, 2020, 2191
PspCI CACGTG 1 cut(s) 2594
PspFI CCCAGC 2 cut(s) 1135, 1963
PspGI CCWGG 3 cut(s) 850, 2020, 2191
PspN4I GGNNCC 3 cut(s) 1102, 2019, 2055
PspPI GGNCC 3 cut(s) 1101, 1138, 2054
PstNI CAGNNNCTG 2 cut(s) 1973, 2876
PsuI RGATCY 7 cut(s) 685, 760, 786, 1065, 1204, 1683, 2017
PsyI GACNNNGTC 1 cut(s) 1406
PvuII CAGCTG 2 cut(s) 1724, 2587
RsaI GTAC 3 cut(s) 912, 1796, 2356
RsaNI GTAC 3 cut(s) 911, 1795, 2355
RseI CAYNNNNRTG 4 cut(s) 251, 2048, 2292, 2384
SacI GAGCTC 3 cut(s) 1731, 2188, 2448
Sau96I GGNCC 3 cut(s) 1101, 1138, 2054
ScaI AGTACT 1 cut(s) 1796
SchI GAGTC 5 cut(s) 58, 530, 1025, 2343, 2867
ScrFI CCNGG 4 cut(s) 852, 1880, 2022, 2193
SduI GDGCHC 6 cut(s) 961, 1336, 1620, 1731, 2188, 2448
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 9 cut(s) 334, 448, 785, 1099, 1122, 1329, 2425, 2632, 2863
SfcI CTRYAG 2 cut(s) 1455, 2031
Sfr274I CTCGAG 1 cut(s) 1679
SfuI TTCGAA 2 cut(s) 993, 1448
SinI GGWCC 1 cut(s) 2054
SlaI CTCGAG 1 cut(s) 1679
SmiMI CAYNNNNRTG 4 cut(s) 251, 2048, 2292, 2384
SmlI CTYRAG 7 cut(s) 811, 915, 1016, 1679, 1798, 2346, 2752
SmoI CTYRAG 7 cut(s) 811, 915, 1016, 1679, 1798, 2346, 2752
SphI GCATGC 1 cut(s) 2165
SseBI AGGCCT 1 cut(s) 2248
SstI GAGCTC 3 cut(s) 1731, 2188, 2448
StuI AGGCCT 1 cut(s) 2248
StyD4I CCNGG 4 cut(s) 850, 1878, 2020, 2191
StyI CCWWGG 3 cut(s) 1104, 1212, 2668
TaiI ACGT 4 cut(s) 1156, 1702, 2356, 2596
TaqII GACCGA 2 cut(s) 1943, 2245
TatI WGTACW 1 cut(s) 1794
TauI GCSGC 4 cut(s) 32, 107, 175, 1421
TfiI GAWTC 5 cut(s) 650, 805, 1552, 1875, 2342
TscAI CASTG 7 cut(s) 55, 555, 1378, 1616, 1974, 2119, 2364
TseFI GTSAC 5 cut(s) 276, 1024, 1969, 2335, 2800
TseI GCWGC 8 cut(s) 461, 1346, 1421, 1721, 1756, 2483, 2645, 2891
Tsp45I GTSAC 5 cut(s) 276, 1024, 1969, 2335, 2800
TspGWI ACGGA 4 cut(s) 74, 1268, 1752, 2654
TspRI CASTG 7 cut(s) 55, 555, 1378, 1616, 1974, 2119, 2364
Tth111I GACNNNGTC 1 cut(s) 1406
Van91I CCANNNNNTGG 3 cut(s) 857, 2320, 2687
Vha464I CTTAAG 2 cut(s) 1016, 1798
VpaK11BI GGWCC 1 cut(s) 2054
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 3 cut(s) 242, 989, 2507
XbaI TCTAGA 2 cut(s) 409, 1538
XceI RCATGY 1 cut(s) 2165
XcmI CCANNNNNNNNNTGG 2 cut(s) 1076, 1432
XhoI CTCGAG 1 cut(s) 1679
XmaJI CCTAGG 1 cut(s) 2668
ZrmI AGTACT 1 cut(s) 1796
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.