MD09G1061700.v1.1

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009881
Physical Location & Seq
Reverse (-)
4205077 .. 4210373
5297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1061700.v1.1.491

Sequence Viewer

Length: 2952 bp
ATGGCGTCGGCGGCCTCGTTCGCTTCCGGGGTCGGTCTACTTCATCTTCCCCAATCTGTTTCTCCCAAATGCTGCAATAAAGCTTCCGTCTTTGCCCGCCCCCACCACTCCCTCAGCTTCAATTCCCAACCCGACTCCTTCCGGGCCTTAATTTCGAGGCAATCGACTCCGAACGGTGCGTTCCGAACTGGGCTGCGGCGACTCGGTCGGAATTCGAGGCCTTTCGTCGTGCGCTGTGATGCTTCGACTGGAAATGGAAGGATTACACAGCAGGATTTTACAGAAATGGCGTGGCAAGCGATTGTTTCGTCGCCGGAAGTGGCGAAAGAGAACAAGCATCAGATAGTGGAGACTGAGCATTTGTTGAAGGCACTCTTGGAGCAGAAGAATGGTCTTGCTCGTCGGATTTTCTCCAAGGCCGGGGTCGACAATACCCGGCTTCTCGAGGCCACTGATAAGTATATTCAACGACAACCAAAGGTTCTTGGTGAATCTTCGGGGTCAGTGTTGGGACGTGATTTGGAAGCCTTGATTCAGCGAGCCAGGGATTACAAGAAAGAGTATGGGGACTCATTTGTGTCTGTGGAGCATTTGGTTCTTGGTTTCGCCCAAGATCAACGATTCGGGAAGCAATTATTTAGGGATTTTCAATTAACCAAGGAAAGCTTGAAATCTGCTATAGAATCCATAAGGGGACGTCAATCAGTTATTGACCAAGATCCTGAAGGGAAATACGAAGCACTTGAAAGGTACGGAAAAGATTTAACAGCTATGGCAAAGGCAGGAAAGCTTGACCCCGTGATAGGAAGAGATGATGAAATTCGCAGGTGCATCCAGATTCTCTCCAGAAGAACAAAGAACAATCCGGTGCTGATTGGGGAACCAGGTGTTGGAAAAACTGCAATTTCCGAAGGGCTTGCTCAAAGAATTGTGCAAGGGGACGTCCCACAAGCTCTGATGAATCGTAAGTTGATATCCCTTGACATGGGATCGCTGATTGCTGGGGCAAAATATCGGGGAGAATTTGAGGACAGGCTGAAGGCAGTACTCAGGGAAGTAACAGAATCAGAGGGCCAGATAATCCTTTTTATTGATGAGATCCACACAGTTGTTGGGGCAGGTGCTACAAACGGTGCAATGGATGCTGGTAATCTCTTAAAACCCATGCTTGGTCGAGGAGAGTTGCGGTGTATAGGTGCGACGACGCTGGATGAGTATCGCAAATATATTGAGAAAGATCCGGCATTGGAGCGTCGGTTCCAGCAAGTTTATGTTGATCAACCTACAGTAGAAGATACCATTTCAATACTCCGGGGACTGCGTGAGAGATACGAGTTGCATCATGGAGTCCGAATTTCTGATGGTGCACTGGTGGAAGCTGCAATTCTCTCAGACCGCTACATCAGTGGGCGATTTTTACCTGATAAAGCAATTGACTTGGTTGATGAAGCTGCTGCTAAATTGAAAATGGAGATTACGTCAAAGCCCACGGCCCTAGATGAGATCAATCGTTCAGTGTTGAAACTTGAAATGGAGAGATTGTCCCTTACAAATGATACGGACAAAGCTTCAAAAGAGAGATTGAACCGCCTTGAGGCTGAGTTATCTCTCTTAAAAGAAAAACAATCCGAGTTGGCTGAGCAGTGGGAGCATGAAAAATCTGTCATGACTCGCATTCAGTCAATCAAGGAAGAGATTGACAGAGTAAATCTTGAGATCCAGCAAGCCGAACGAGAGTATGATCTCAACCGTGCTGCTGAGTTGAAGTACGGGAGTCTGAATTCTTTGCAGCGACAACTCGGTGGTGCAGAAAAAGAGCTTGATGAGTATATGAGGTCTGGGAAGTCCATGCTGAGAGAGGAAGTTACTGGAAGTGATATTGCTGAAATAGTAAGTAAGTGGACCGGTATTCCTGTTTCCAAGCTTCAACAATCGGAGAGGGAGAAGCTCTTACATTTGGAGGATGAGCTGCATAAACGTGTTGTAGGTCAGGATCCTGCAGTGAGATCAGTCGCTGAAGCTATTCAGCGGTCTCGGGCAGGTCTCTCGGATCCTCATCGCCCTATTGCTAGTTTCATGTTCATGGGTCCCACTGGTGTTGGGAAGACAGAACTAGCTAAGGCCCTTGCCTCCTACATGTTCAACACAGAAGAAGCACTCGTAAGAATCGATATGAGCGAGTACATGGAAAAGCATGCTGTTTCGAGATTGATAGGAGCTCCACCTGGTTATGTGGGGTATGAGGAGGGAGGACAGCTGACAGAGGTGGTTCGCCGGAGACCGTATTCAGTTATTTTGTTCGATGAGATAGAGAAGGCACATTCTGATGTGTTCAACGTGTTCCTTCAAATTTTAGACGATGGGAGAATAACCGACTCACAGGGTCGCACTGTGAGTTTCACCAACACTGTCATCATCATGACCTCAAATGTGGGCTCACAGTACATACTAAACGCTGATGATGACAGCACTCCAAGGGAGTTGGCTTACGAAACTATAAAGCAGCGGGTAATGGAGGCTGCAAGATCAATATTCCGTCCTGAGTTCATGAACCGGGTTGATGAGTACATAGTTTTCCAGCCCTTGGACCGCGATCAGATAAGCCGTATTGTCAAGTTACAGTTGGACCGAGTGCAAAAGAGAATTGCAGACCGGAAGATGAAGCTCAAGGTGAGCGATGCAGCTCTCCAGCTTCTCGGAAGTCTTGGGTATGATCCAAACTACGGTGCTAGGCCGGTGAAGCGGGTGATACAGCAATATGTCGAAAACGAGCTTGCCAAGGGCATATTGAGAGGAGAGTTTAGGGAGGAAGACACTGTTTTTATCGACACAGAGGTCACGGCATTTTCCAATGGCCAGCTCCCCCAGCAAAAGCTAGTGTTCAGGACGTTAGAAACAGACTCAGAATCTCCGGCTACAGAGAACCAAGAAGCTTTGTCGGAGACTCGATAG

Protein Analysis

984

Amino Acids

110.53

Weight (kDa)

6.16

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 93 - 213 3.2e-35 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 272 - 373 2.9e-06 SMAX1 nucleotide binding domain
AAA PF00004 290 - 421 1.5e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 429 - 531 7.3e-34 AAA lid domain
Sigma54_activat PF00158 684 - 810 3e-06 Sigma-54 interaction domain
AAA_2 PF07724 687 - 855 5.3e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 692 - 813 2.5e-11 AAA domain (dynein-related subfamily)
AAA PF00004 693 - 809 2.9e-09 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 862 - 941 2.9e-27 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 816, 1109
AasI GACNNNNNNGTC 1 cut(s) 2834
AatII GACGTC 2 cut(s) 700, 945
Acc36I ACCTGC 3 cut(s) 816, 1109, 2030
AccB7I CCANNNNNTGG 2 cut(s) 890, 2584
AccI GTMKAC 2 cut(s) 37, 426
AccII CGCG 1 cut(s) 2592
AcoI YGGCCR 1 cut(s) 2854
AcsI RAATTY 6 cut(s) 211, 819, 1022, 1353, 1780, 2349
AcuI CTGAAG 3 cut(s) 744, 1058, 2037
AcyI GRCGYC 3 cut(s) 5, 697, 942
AfaI GTAC 6 cut(s) 752, 1047, 1769, 2183, 2444, 2567
AfiI CCNNNNNNNGG 8 cut(s) 420, 803, 890, 985, 1169, 1594, 2584, 2723
AflIII ACRYGT 3 cut(s) 1978, 2136, 2337
AgeI ACCGGT 1 cut(s) 1904
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 3 cut(s) 542, 883, 2224
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 873, 905
AloI GAACNNNNNNTCC 2 cut(s) 1241, 1273
Alw21I GWGCWC 2 cut(s) 1369, 2221
Alw26I GTCTC 5 cut(s) 344, 2037, 2048, 2272, 2936
Alw44I GTGCAC 1 cut(s) 1365
AlwNI CAGNNNCTG 1 cut(s) 2015
Ama87I CYCGRG 2 cut(s) 443, 2034
ApaLI GTGCAC 1 cut(s) 1365
ApoI RAATTY 6 cut(s) 211, 819, 1022, 1353, 1780, 2349
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AsiGI ACCGGT 1 cut(s) 1904
Asp700I GAANNNNTTC 1 cut(s) 2022
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 8 cut(s) 144, 1072, 1492, 1902, 2087, 2122, 2587, 2626
AsuC2I CCSGG 6 cut(s) 28, 143, 421, 436, 1313, 2555
AsuHPI GGTGA 5 cut(s) 500, 2392, 2683, 2749, 2758
AvaI CYCGRG 2 cut(s) 443, 2034
AvaII GGWCC 4 cut(s) 1902, 2087, 2587, 2626
BaeGI GKGCMC 1 cut(s) 1369
BalI TGGCCA 1 cut(s) 2856
BamHI GGATCC 2 cut(s) 1993, 2050
BanII GRGCYC 2 cut(s) 2221, 2438
BbsI GAAGAC 2 cut(s) 2111, 2817
Bbv12I GWGCWC 2 cut(s) 1369, 2221
BbvCI CCTCAGC 1 cut(s) 113
BccI CCATC 2 cut(s) 1355, 2354
BceAI ACGGC 3 cut(s) 1506, 2589, 2856
BcgI CGANNNNNNTGC 4 cut(s) 899, 933, 2028, 2062
BciT130I CCWGG 3 cut(s) 544, 885, 2226
BclI TGATCA 1 cut(s) 1276
BcnI CCSGG 6 cut(s) 28, 143, 421, 436, 1313, 2555
BcoDI GTCTC 5 cut(s) 344, 2037, 2048, 2272, 2936
BfaI CTAG 5 cut(s) 1496, 2070, 2114, 2730, 2876
BfmI CTRYAG 4 cut(s) 678, 1284, 1998, 2916
BfuAI ACCTGC 3 cut(s) 816, 1109, 2030
BlpI GCTNAGC 1 cut(s) 1638
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 9 cut(s) 28, 143, 421, 436, 544, 885, 1313, 2226, 2555
Bme18I GGWCC 4 cut(s) 1902, 2087, 2587, 2626
BmeT110I CYCGRG 2 cut(s) 443, 2034
BmgBI CACGTC 1 cut(s) 515
BmgT120I GGNCC 8 cut(s) 144, 1072, 1492, 1902, 2087, 2122, 2587, 2626
BmiI GGNNCC 6 cut(s) 882, 1259, 1995, 2052, 2088, 2089
BmrFI CCNGG 9 cut(s) 28, 143, 421, 436, 544, 885, 1313, 2226, 2555
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 6 cut(s) 229, 346, 840, 1132, 1348, 2668
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 2111, 2817
BpmI CTGGAG 2 cut(s) 829, 2672
Bpu10I CCTNAGC 2 cut(s) 113, 2118
Bpu1102I GCTNAGC 1 cut(s) 1638
BpuEI CTTGAG 3 cut(s) 1613, 1733, 2651
BpuMI CCSGG 6 cut(s) 28, 143, 421, 436, 1313, 2555
Bsa29I ATCGAT 1 cut(s) 2169
BsaBI GATNNNNATC 2 cut(s) 1215, 2055
BsaHI GRCGYC 3 cut(s) 5, 697, 942
BsaI GGTCTC 3 cut(s) 2037, 2048, 2272
BsaWI WCCGGW 3 cut(s) 865, 1904, 2652
BsaXI ACNNNNNCTCC 6 cut(s) 1241, 1271, 1463, 1493, 1526, 1556
Bsc4I CCNNNNNNNGG 8 cut(s) 420, 803, 890, 985, 1169, 1594, 2584, 2723
Bse118I RCCGGY 2 cut(s) 1904, 2734
Bse1I ACTGG 5 cut(s) 193, 253, 1374, 1873, 2098
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 2 cut(s) 1215, 2055
BseBI CCWGG 3 cut(s) 544, 885, 2226
BseCI ATCGAT 1 cut(s) 2169
BseGI GGATG 4 cut(s) 831, 1147, 1216, 1969
BseJI GATNNNNATC 2 cut(s) 1215, 2055
BseLI CCNNNNNNNGG 8 cut(s) 420, 803, 890, 985, 1169, 1594, 2584, 2723
BseMI GCAATG 1 cut(s) 1143
BseNI ACTGG 5 cut(s) 193, 253, 1374, 1873, 2098
BseRI GAGGAG 3 cut(s) 1191, 2258, 2808
BseSI GKGCMC 1 cut(s) 1369
BseYI CCCAGC 2 cut(s) 1001, 2865
BsgI GTGCAG 1 cut(s) 1827
Bsh1236I CGCG 1 cut(s) 2592
Bsh1285I CGRYCG 1 cut(s) 208
BshTI ACCGGT 1 cut(s) 1904
BshVI ATCGAT 1 cut(s) 2169
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 1369, 2221
BsiHKCI CYCGRG 2 cut(s) 443, 2034
BslFI GGGAC 8 cut(s) 525, 581, 708, 929, 953, 1329, 1528, 2073
BslI CCNNNNNNNGG 8 cut(s) 420, 803, 890, 985, 1169, 1594, 2584, 2723
BsmAI GTCTC 5 cut(s) 344, 2037, 2048, 2272, 2936
BsmFI GGGAC 8 cut(s) 525, 581, 708, 929, 953, 1329, 1528, 2073
BsmI GAATGC 1 cut(s) 1674
Bso31I GGTCTC 3 cut(s) 2037, 2048, 2272
BsoBI CYCGRG 2 cut(s) 443, 2034
Bsp1286I GDGCHC 3 cut(s) 1369, 2221, 2438
Bsp1720I GCTNAGC 1 cut(s) 1638
BspDI ATCGAT 1 cut(s) 2169
BspFNI CGCG 1 cut(s) 2592
BspHI TCATGA 3 cut(s) 1665, 2418, 2547
BspLI GGNNCC 6 cut(s) 882, 1259, 1995, 2052, 2088, 2089
BspMAI CTGCAG 1 cut(s) 2002
BspMI ACCTGC 3 cut(s) 816, 1109, 2030
BspTNI GGTCTC 3 cut(s) 2037, 2048, 2272
BsrDI GCAATG 1 cut(s) 1143
BsrFI RCCGGY 2 cut(s) 1904, 2734
BsrI ACTGG 5 cut(s) 193, 253, 1374, 1873, 2098
BssAI RCCGGY 2 cut(s) 1904, 2734
BssNI GRCGYC 3 cut(s) 5, 697, 942
BssT1I CCWWGG 5 cut(s) 414, 657, 2474, 2583, 2778
Bst2UI CCWGG 3 cut(s) 544, 885, 2226
Bst6I CTCTTC 2 cut(s) 802, 1686
BstACI GRCGYC 3 cut(s) 5, 697, 942
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstC8I GCNNGC 8 cut(s) 97, 297, 540, 918, 1725, 2196, 2775, 2858
BstDSI CCRYGG 1 cut(s) 1488
BstF5I GGATG 4 cut(s) 831, 1147, 1216, 1969
BstFNI CGCG 1 cut(s) 2592
BstHHI GCGC 1 cut(s) 234
BstMAI GTCTC 5 cut(s) 344, 2037, 2048, 2272, 2936
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 296, 1142, 1648, 2740, 2866
BstNI CCWGG 3 cut(s) 544, 885, 2226
BstNSI RCATGY 2 cut(s) 2140, 2198
BstSCI CCNGG 9 cut(s) 26, 141, 419, 434, 542, 883, 1311, 2224, 2553
BstSFI CTRYAG 4 cut(s) 678, 1284, 1998, 2916
BstSLI GKGCMC 1 cut(s) 1369
BstUI CGCG 1 cut(s) 2592
BstV2I GAAGAC 2 cut(s) 2111, 2817
BstX2I RGATCY 6 cut(s) 718, 1098, 1237, 1716, 1993, 2050
BstYI RGATCY 6 cut(s) 718, 1098, 1237, 1716, 1993, 2050
Bsu15I ATCGAT 1 cut(s) 2169
BsuTUI ATCGAT 1 cut(s) 2169
BtgI CCRYGG 1 cut(s) 1488
BtgZI GCGATG 2 cut(s) 2042, 2691
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 4 cut(s) 831, 1147, 1216, 1969
BtsI GCAGTG 2 cut(s) 1649, 2007
BveI ACCTGC 3 cut(s) 816, 1109, 2030
Cac8I GCNNGC 8 cut(s) 97, 297, 540, 918, 1725, 2196, 2775, 2858
CaiI CAGNNNCTG 1 cut(s) 2015
CciI TCATGA 3 cut(s) 1665, 2418, 2547
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 2 cut(s) 1904, 2734
Cfr13I GGNCC 8 cut(s) 144, 1072, 1492, 1902, 2087, 2122, 2587, 2626
ClaI ATCGAT 1 cut(s) 2169
CseI GACGC 2 cut(s) 1213, 1241
CsiI ACCWGGT 2 cut(s) 883, 2224
Csp6I GTAC 6 cut(s) 751, 1046, 1768, 2182, 2443, 2566
CspAI ACCGGT 1 cut(s) 1904
CspCI CAANNNNNGTGG 2 cut(s) 2080, 2115
CviQI GTAC 6 cut(s) 751, 1046, 1768, 2182, 2443, 2566
DrdI GACNNNNNNGTC 1 cut(s) 2834
DseDI GACNNNNNNGTC 1 cut(s) 2834
EaeI YGGCCR 1 cut(s) 2854
Eam1104I CTCTTC 2 cut(s) 802, 1686
EarI CTCTTC 2 cut(s) 802, 1686
Ecl136II GAGCTC 1 cut(s) 2219
Eco130I CCWWGG 5 cut(s) 414, 657, 2474, 2583, 2778
Eco147I AGGCCT 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 2221, 2438
Eco31I GGTCTC 3 cut(s) 2037, 2048, 2272
Eco32I GATATC 1 cut(s) 975
Eco47I GGWCC 4 cut(s) 1902, 2087, 2587, 2626
Eco53kI GAGCTC 1 cut(s) 2219
Eco57I CTGAAG 3 cut(s) 744, 1058, 2037
Eco88I CYCGRG 2 cut(s) 443, 2034
EcoICRI GAGCTC 1 cut(s) 2219
EcoO109I RGGNCCY 2 cut(s) 2087, 2122
EcoRI GAATTC 2 cut(s) 211, 1780
EcoRII CCWGG 3 cut(s) 542, 883, 2224
EcoRV GATATC 1 cut(s) 975
EcoT14I CCWWGG 5 cut(s) 414, 657, 2474, 2583, 2778
EcoT38I GRGCYC 2 cut(s) 2221, 2438
ErhI CCWWGG 5 cut(s) 414, 657, 2474, 2583, 2778
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 650, 682
FaqI GGGAC 8 cut(s) 525, 581, 708, 929, 953, 1329, 1528, 2073
FauI CCCGC 3 cut(s) 104, 2499, 2736
FbaI TGATCA 1 cut(s) 1276
FblI GTMKAC 2 cut(s) 37, 426
FokI GGATG 4 cut(s) 818, 1154, 1223, 1976
FriOI GRGCYC 2 cut(s) 2221, 2438
FspBI CTAG 5 cut(s) 1496, 2070, 2114, 2730, 2876
GlaI GCGC 1 cut(s) 233
GsaI CCCAGC 2 cut(s) 1005, 2869
GsuI CTGGAG 2 cut(s) 829, 2672
HgaI GACGC 2 cut(s) 1213, 1241
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 3 cut(s) 5, 697, 942
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 6 cut(s) 81, 664, 788, 1566, 1922, 2931
HphI GGTGA 5 cut(s) 500, 2392, 2683, 2749, 2758
Hpy166II GTNNAC 4 cut(s) 38, 427, 1367, 1902
Hpy8I GTNNAC 4 cut(s) 38, 427, 1367, 1902
Hpy99I CGWCG 7 cut(s) 10, 230, 313, 405, 1204, 1207, 1257
HpyAV CCTTC 8 cut(s) 148, 252, 361, 719, 905, 1033, 2308, 2354
HpyCH4IV ACGT 7 cut(s) 514, 697, 942, 1478, 1978, 2337, 2888
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 296, 1142, 1648, 2740, 2866
HpySE526I ACGT 7 cut(s) 514, 697, 942, 1478, 1978, 2337, 2888
Hsp92I GRCGYC 3 cut(s) 5, 697, 942
HspAI GCGC 1 cut(s) 232
KflI GGGWCCC 1 cut(s) 2087
Ksp22I TGATCA 1 cut(s) 1276
LmnI GCTCC 7 cut(s) 379, 586, 1249, 1648, 2216, 2224, 2865
LweI GCATC 6 cut(s) 229, 346, 840, 1132, 1348, 2668
MabI ACCWGGT 2 cut(s) 883, 2224
MaeI CTAG 5 cut(s) 1496, 2070, 2114, 2730, 2876
MaeII ACGT 7 cut(s) 514, 697, 942, 1478, 1978, 2337, 2888
MaeIII GTNAC 4 cut(s) 1057, 1864, 2616, 2836
MfeI CAATTG 1 cut(s) 1431
MflI RGATCY 6 cut(s) 718, 1098, 1237, 1716, 1993, 2050
MhlI GDGCHC 3 cut(s) 1369, 2221, 2438
MlsI TGGCCA 1 cut(s) 2856
MluNI TGGCCA 1 cut(s) 2856
MmeI TCCRAC 5 cut(s) 188, 383, 871, 2604, 2919
Mox20I TGGCCA 1 cut(s) 2856
MroXI GAANNNNTTC 1 cut(s) 2022
MscI TGGCCA 1 cut(s) 2856
MseI TTAA 5 cut(s) 149, 653, 764, 1157, 1613
MslI CAYNNNNRTG 4 cut(s) 1977, 2081, 2325, 2417
Msp20I TGGCCA 1 cut(s) 2856
MspA1I CMGCKG 3 cut(s) 2029, 2257, 2506
MspR9I CCNGG 9 cut(s) 28, 143, 421, 436, 544, 885, 1313, 2226, 2555
MunI CAATTG 1 cut(s) 1431
Mva1269I GAATGC 1 cut(s) 1674
MvaI CCWGG 3 cut(s) 544, 885, 2226
MvnI CGCG 1 cut(s) 2592
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 296, 1142, 1648, 2740, 2866
NciI CCSGG 6 cut(s) 28, 143, 421, 436, 1313, 2555
NlaIV GGNNCC 6 cut(s) 882, 1259, 1995, 2052, 2088, 2089
NmuCI GTSAC 1 cut(s) 2836
NspI RCATGY 2 cut(s) 2140, 2198
PaeI GCATGC 1 cut(s) 2198
PaeR7I CTCGAG 1 cut(s) 443
PagI TCATGA 3 cut(s) 1665, 2418, 2547
PaqCI CACCTGC 2 cut(s) 816, 1109
PceI AGGCCT 1 cut(s) 220
PciI ACATGT 1 cut(s) 2136
PcsI WCGNNNNNNNCGW 4 cut(s) 14, 161, 2166, 2175
PctI GAATGC 1 cut(s) 1674
PdmI GAANNNNTTC 1 cut(s) 2022
PfeI GAWTC 9 cut(s) 491, 532, 621, 683, 838, 961, 1064, 2166, 2906
PflFI GACNNNGTC 1 cut(s) 204
PflMI CCANNNNNTGG 2 cut(s) 890, 2584
PinAI ACCGGT 1 cut(s) 1904
PpuMI RGGWCCY 1 cut(s) 2087
PscI ACATGT 1 cut(s) 2136
Psp124BI GAGCTC 1 cut(s) 2221
Psp5II RGGWCCY 1 cut(s) 2087
Psp6I CCWGG 3 cut(s) 542, 883, 2224
PspFI CCCAGC 2 cut(s) 1001, 2865
PspGI CCWGG 3 cut(s) 542, 883, 2224
PspN4I GGNNCC 6 cut(s) 882, 1259, 1995, 2052, 2088, 2089
PspPI GGNCC 8 cut(s) 144, 1072, 1492, 1902, 2087, 2122, 2587, 2626
PspPPI RGGWCCY 1 cut(s) 2087
PstI CTGCAG 1 cut(s) 2002
PstNI CAGNNNCTG 1 cut(s) 2015
PsuI RGATCY 6 cut(s) 718, 1098, 1237, 1716, 1993, 2050
PsyI GACNNNGTC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 2257
RsaI GTAC 6 cut(s) 752, 1047, 1769, 2183, 2444, 2567
RsaNI GTAC 6 cut(s) 751, 1046, 1768, 2182, 2443, 2566
RseI CAYNNNNRTG 4 cut(s) 1977, 2081, 2325, 2417
SacI GAGCTC 1 cut(s) 2221
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 5 cut(s) 149, 653, 764, 1157, 1613
Sau96I GGNCC 8 cut(s) 144, 1072, 1492, 1902, 2087, 2122, 2587, 2626
ScaI AGTACT 1 cut(s) 1047
ScrFI CCNGG 9 cut(s) 28, 143, 421, 436, 544, 885, 1313, 2226, 2555
SduI GDGCHC 3 cut(s) 1369, 2221, 2438
SexAI ACCWGGT 2 cut(s) 883, 2224
SfaNI GCATC 6 cut(s) 229, 346, 840, 1132, 1348, 2668
SfcI CTRYAG 4 cut(s) 678, 1284, 1998, 2916
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 4 cut(s) 1902, 2087, 2587, 2626
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 4 cut(s) 1977, 2081, 2325, 2417
SmlI CTYRAG 4 cut(s) 443, 1592, 1712, 2666
SmoI CTYRAG 4 cut(s) 443, 1592, 1712, 2666
SphI GCATGC 1 cut(s) 2198
SseBI AGGCCT 1 cut(s) 220
SspI AATATT 1 cut(s) 2532
SspMI CTAG 5 cut(s) 1496, 2070, 2114, 2730, 2876
SstI GAGCTC 1 cut(s) 2221
StuI AGGCCT 1 cut(s) 220
StyD4I CCNGG 9 cut(s) 26, 141, 419, 434, 542, 883, 1311, 2224, 2553
StyI CCWWGG 5 cut(s) 414, 657, 2474, 2583, 2778
TaiI ACGT 7 cut(s) 517, 700, 945, 1481, 1981, 2340, 2891
TaqII GACCGA 3 cut(s) 23, 194, 2643
TatI WGTACW 4 cut(s) 1045, 2181, 2442, 2565
TauI GCSGC 2 cut(s) 14, 199
TfiI GAWTC 9 cut(s) 491, 532, 621, 683, 838, 961, 1064, 2166, 2906
Tru1I TTAA 5 cut(s) 149, 653, 764, 1157, 1613
Tru9I TTAA 5 cut(s) 149, 653, 764, 1157, 1613
TseFI GTSAC 1 cut(s) 2836
Tsp45I GTSAC 1 cut(s) 2836
TspGWI ACGGA 4 cut(s) 76, 768, 1574, 2525
Tth111I GACNNNGTC 1 cut(s) 204
Van91I CCANNNNNTGG 2 cut(s) 890, 2584
VneI GTGCAC 1 cut(s) 1365
VpaK11BI GGWCC 4 cut(s) 1902, 2087, 2587, 2626
XapI RAATTY 6 cut(s) 211, 819, 1022, 1353, 1780, 2349
XceI RCATGY 2 cut(s) 2140, 2198
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XhoI CTCGAG 1 cut(s) 443
XmiI GTMKAC 2 cut(s) 37, 426
XmnI GAANNNNTTC 1 cut(s) 2022
XspI CTAG 5 cut(s) 1496, 2070, 2114, 2730, 2876
ZraI GACGTC 2 cut(s) 698, 943
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.