Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009881

Physical Location & Seq

Genomic Coordinates Forward (+)

39545983 .. 39548678

Gene Structure 2696 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1172100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCGTCGGCGACCTCGCTCTCTTCTGGGGTCGCTCTTGTTCATCTTCCCCAATTTCCGAAATGCTGCGATAAAGCTTCCGTCTTTGCCCGCCCCCACCACTCCCTCAGCTTCAATTCCCGTGCCGACTCCTTTCAGGCGTTCATTTCACGGCAGTTGACTCCGAACGGTTCGTTCCGAACCGGGTTGTGGAGAACTAATCGGAATTCGAGGCCTTTCGTCGTGCGCTGTGAAGCTTCAACTGGAAGGGGAAGGATTACACAGCAGGATTTTACAGAAATGGCGTGGCAAGCAATTGTTTCGTCGCCGGAAGTGGCGAAAGAGAACAAGCATCAGATAGTGGAGACTGAACATCTGTTGAAGGCACTCTTGGAGCAGAAGAATGGTCTTGCTCGTCGGATTTTTTCCAAGGTTGGGGTGGACAATGCCCGGCTTCTCGAGGCCACTGATAAGTATATTCAGCGCCAACCAAAGGTTTTTGGTGAATCTGCGGGCTCAATGTTGGGACGTGATTTGGAAGCCTTGATTCAGCGAGCCAGGGATTACAAGAAAGAGTATGGGGACTCATTTGTGTCTGTCGAGCATTTGGTACTTGGTTTTTCTCAAGATCAACGCTTTGGGAAGCAATTATTTCGGGATTTTCAAATATCCAAGGAAGCTTTGAAATCTGCTATAGAATCCATAAGGGGGCGCCAATCAGTTATTGACCAAGATCCTGAAGGGAAATATGAAGCACTTGAAAAGTACGGAAAAGATTTAACAGCTATGGCAAAAGTAGGAAAGCTTGACCCAGTAATAGGAAGAGATGATGAAATTCGTAGGTGCATCCAGATTCTCTCCAGAAGAACAAAGAACAACCCGGTGCTGATTGGTGAACCAGGTGTCGGAAAAACTGCAATTTCTGAAGGGCTTGCTCAAAGAATCGTGCAAGGGGATGTCCCACAAGCTCTGATGAATCGTAAGTTAATATCCCTTGACATGGGCGCACTAATTGCTGGGGCAAAATATCGGGGAGAATTTGAGGACAGGCTGAAGGCAGTACTCAGGGAAGTAACAGAATCAGAGGGCCAGATAATCCTTTTTATCGATGAGATCCACACAGTTGTTGGGGCAGGTATTAAGGTGCTACAAACGGTGCAATGGATGCTGGTAATCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006996 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

387

Amino Acids

43.1

Weight (kDa)

9.39

Isoelectric Point (pI)

33.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	91 - 211	1.7e-35	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	270 - 369	1.6e-06	SMAX1 nucleotide binding domain
AAA	PF00004	288 - 372	2.2e-11	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15450
fragaria_vesca	FvH4_6g47410 FvH4_6g47410 FvH4_6g47410
malus_domestica	MD00G1172100.v1.1 MD09G1061700.v1.1
prunus_persica	Prupe.3G258500_v2.0.a1 Prupe.3G258500_v2.0.a1 Prupe.3G258500_v2.0.a1
pyrus_communis	pycom111g05200 pycom17g05590
rosa_chinensis	RchiOBHm_Chr2g0166481
rosa_laevigata	RLG00000021622
rosa_multiflora	Rmu_ssc0000296.1_g000002
rosa_roxburghii	Rroxscaffold_2G00084860
rosa_rugosa	Rorug02G0523200
rosa_samantha	Rh2AG589400 Rh2BG601600 Rh2CG572200 Rh2DG612100
rosa_wichuraiana	Rw2G049120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1103
AccB1I	GGYRCC	1 cut(s)	690
AciI	CCGC	2 cut(s)	91, 491
AclWI	GGATC	2 cut(s)	707, 1087
AcsI	RAATTY	3 cut(s)	205, 813, 1016
AcuI	CTGAAG	3 cut(s)	738, 924, 1052
AcyI	GRCGYC	2 cut(s)	5, 691
AfaI	GTAC	3 cut(s)	591, 746, 1041
AfiI	CCNNNNNNNGG	7 cut(s)	189, 414, 472, 687, 797, 884, 979
AgsI	TTSAA	6 cut(s)	115, 240, 361, 644, 664, 740
AjiI	CACGTC	1 cut(s)	509
AjnI	CCWGG	2 cut(s)	536, 877
AjuI	GAANNNNNNNTTGG	2 cut(s)	353, 385
AluBI	AGCT	7 cut(s)	77, 111, 236, 659, 764, 784, 947
AluI	AGCT	7 cut(s)	77, 111, 236, 659, 764, 784, 947
Alw26I	GTCTC	1 cut(s)	338
AlwI	GGATC	2 cut(s)	707, 1087
Ama87I	CYCGRG	1 cut(s)	437
AoxI	GGCC	3 cut(s)	212, 441, 1066
ApeKI	GCWGC	1 cut(s)	66
ApoI	RAATTY	3 cut(s)	205, 813, 1016
AspLEI	GCGC	4 cut(s)	228, 465, 693, 986
AspS9I	GGNCC	1 cut(s)	1066
AsuC2I	CCSGG	3 cut(s)	184, 430, 860
AsuHPI	GGTGA	2 cut(s)	494, 884
AvaI	CYCGRG	1 cut(s)	437
BanI	GGYRCC	1 cut(s)	690
BanII	GRGCYC	1 cut(s)	497
BbvCI	CCTCAGC	1 cut(s)	107
BbvI	GCAGC	1 cut(s)	53
BceAI	ACGGC	1 cut(s)	167
BcgI	CGANNNNNNTGC	4 cut(s)	282, 316, 614, 648
BciT130I	CCWGG	2 cut(s)	538, 879
BcnI	CCSGG	3 cut(s)	184, 430, 860
BcoDI	GTCTC	1 cut(s)	338
BfmI	CTRYAG	1 cut(s)	672
BfoI	RGCGCY	2 cut(s)	466, 694
BfuAI	ACCTGC	1 cut(s)	1103
BisI	GCNGC	1 cut(s)	67
BlsI	GCNGC	1 cut(s)	68
BmcAI	AGTACT	1 cut(s)	1041
Bme1390I	CCNGG	5 cut(s)	184, 430, 538, 860, 879
BmeT110I	CYCGRG	1 cut(s)	437
BmgBI	CACGTC	1 cut(s)	509
BmgT120I	GGNCC	1 cut(s)	1066
BmiI	GGNNCC	1 cut(s)	692
BmrFI	CCNGG	5 cut(s)	184, 430, 538, 860, 879
BmrI	ACTGGG	1 cut(s)	785
BmsI	GCATC	3 cut(s)	340, 834, 1134
BmuI	ACTGGG	1 cut(s)	785
BpmI	CTGGAG	1 cut(s)	823
Bpu10I	CCTNAGC	1 cut(s)	107
BpuEI	CTTGAG	1 cut(s)	588
BpuMI	CCSGG	3 cut(s)	184, 430, 860
Bsa29I	ATCGAT	1 cut(s)	1086
BsaHI	GRCGYC	2 cut(s)	5, 691
BsaJI	CCNNGG	3 cut(s)	408, 537, 651
Bsc4I	CCNNNNNNNGG	7 cut(s)	189, 414, 472, 687, 797, 884, 979
Bse1I	ACTGG	2 cut(s)	247, 791
Bse3DI	GCAATG	1 cut(s)	1145
BseBI	CCWGG	2 cut(s)	538, 879
BseCI	ATCGAT	1 cut(s)	1086
BseDI	CCNNGG	3 cut(s)	408, 537, 651
BseGI	GGATG	3 cut(s)	825, 940, 1149
BseLI	CCNNNNNNNGG	7 cut(s)	189, 414, 472, 687, 797, 884, 979
BseMI	GCAATG	1 cut(s)	1145
BseMII	CTCAG	2 cut(s)	121, 1057
BseNI	ACTGG	2 cut(s)	247, 791
BseXI	GCAGC	1 cut(s)	53
BseYI	CCCAGC	1 cut(s)	995
BshFI	GGCC	3 cut(s)	214, 443, 1068
BshNI	GGYRCC	1 cut(s)	690
BshVI	ATCGAT	1 cut(s)	1086
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	4 cut(s)	183, 308, 430, 860
BslFI	GGGAC	3 cut(s)	519, 575, 923
BslI	CCNNNNNNNGG	7 cut(s)	189, 414, 472, 687, 797, 884, 979
BsmAI	GTCTC	1 cut(s)	338
BsmFI	GGGAC	3 cut(s)	519, 575, 923
BsnI	GGCC	3 cut(s)	214, 443, 1068
BsoBI	CYCGRG	1 cut(s)	437
Bsp1286I	GDGCHC	1 cut(s)	497
Bsp143I	GATC	3 cut(s)	607, 712, 1092
BspACI	CCGC	2 cut(s)	91, 491
BspANI	GGCC	3 cut(s)	214, 443, 1068
BspCNI	CTCAG	2 cut(s)	120, 1056
BspDI	ATCGAT	1 cut(s)	1086
BspLI	GGNNCC	1 cut(s)	692
BspMI	ACCTGC	1 cut(s)	1103
BspPI	GGATC	2 cut(s)	707, 1087
BspT107I	GGYRCC	1 cut(s)	690
BsrDI	GCAATG	1 cut(s)	1145
BsrI	ACTGG	2 cut(s)	247, 791
BssECI	CCNNGG	3 cut(s)	408, 537, 651
BssMI	GATC	3 cut(s)	607, 712, 1092
BssNI	GRCGYC	2 cut(s)	5, 691
BssT1I	CCWWGG	2 cut(s)	408, 651
Bst2UI	CCWGG	2 cut(s)	538, 879
Bst4CI	ACNGT	3 cut(s)	170, 1102, 1135
Bst6I	CTCTTC	2 cut(s)	28, 796
BstACI	GRCGYC	2 cut(s)	5, 691
BstAPI	GCANNNNNTGC	2 cut(s)	992, 1144
BstC8I	GCNNGC	5 cut(s)	91, 291, 493, 534, 912
BstDEI	CTNAG	2 cut(s)	107, 1043
BstF5I	GGATG	3 cut(s)	825, 940, 1149
BstH2I	RGCGCY	2 cut(s)	466, 694
BstHHI	GCGC	4 cut(s)	228, 465, 693, 986
BstKTI	GATC	3 cut(s)	610, 715, 1095
BstMAI	GTCTC	1 cut(s)	338
BstMBI	GATC	3 cut(s)	607, 712, 1092
BstMWI	GCNNNNNNNGC	3 cut(s)	290, 992, 1144
BstNI	CCWGG	2 cut(s)	538, 879
BstSCI	CCNGG	5 cut(s)	182, 428, 536, 858, 877
BstSFI	CTRYAG	1 cut(s)	672
BstV1I	GCAGC	1 cut(s)	53
BstX2I	RGATCY	2 cut(s)	712, 1092
BstYI	RGATCY	2 cut(s)	712, 1092
Bsu15I	ATCGAT	1 cut(s)	1086
BsuRI	GGCC	3 cut(s)	214, 443, 1068
BsuTUI	ATCGAT	1 cut(s)	1086
BtrI	CACGTC	1 cut(s)	509
BtsCI	GGATG	3 cut(s)	825, 940, 1149
BtsIMutI	CAGTG	1 cut(s)	444
BveI	ACCTGC	1 cut(s)	1103
Cac8I	GCNNGC	5 cut(s)	91, 291, 493, 534, 912
CfoI	GCGC	4 cut(s)	228, 465, 693, 986
Cfr13I	GGNCC	1 cut(s)	1066
ClaI	ATCGAT	1 cut(s)	1086
CsiI	ACCWGGT	1 cut(s)	877
Csp6I	GTAC	3 cut(s)	590, 745, 1040
CviAII	CATG	1 cut(s)	979
CviQI	GTAC	3 cut(s)	590, 745, 1040
DdeI	CTNAG	2 cut(s)	107, 1043
DinI	GGCGCC	1 cut(s)	692
DpnI	GATC	3 cut(s)	609, 714, 1094
DpnII	GATC	3 cut(s)	607, 712, 1092
Eam1104I	CTCTTC	2 cut(s)	28, 796
EarI	CTCTTC	2 cut(s)	28, 796
Eco130I	CCWWGG	2 cut(s)	408, 651
Eco147I	AGGCCT	1 cut(s)	214
Eco24I	GRGCYC	1 cut(s)	497
Eco57I	CTGAAG	3 cut(s)	738, 924, 1052
Eco88I	CYCGRG	1 cut(s)	437
EcoRI	GAATTC	1 cut(s)	205
EcoRII	CCWGG	2 cut(s)	536, 877
EcoT14I	CCWWGG	2 cut(s)	408, 651
EcoT38I	GRGCYC	1 cut(s)	497
EgeI	GGCGCC	1 cut(s)	692
EheI	GGCGCC	1 cut(s)	692
ErhI	CCWWGG	2 cut(s)	408, 651
FaeI	CATG	1 cut(s)	982
FaiI	YATR	7 cut(s)	456, 558, 674, 683, 729, 767, 980
FalI	AAGNNNNNCTT	2 cut(s)	353, 385
FaqI	GGGAC	3 cut(s)	519, 575, 923
FatI	CATG	1 cut(s)	978
FauI	CCCGC	2 cut(s)	98, 484
Fnu4HI	GCNGC	1 cut(s)	67
FokI	GGATG	3 cut(s)	812, 947, 1156
FriOI	GRGCYC	1 cut(s)	497
Fsp4HI	GCNGC	1 cut(s)	67
GlaI	GCGC	4 cut(s)	227, 464, 692, 985
GluI	GCNGC	1 cut(s)	67
GsaI	CCCAGC	1 cut(s)	999
GsuI	CTGGAG	1 cut(s)	823
HaeII	RGCGCY	2 cut(s)	466, 694
HaeIII	GGCC	3 cut(s)	214, 443, 1068
HapII	CCGG	4 cut(s)	183, 308, 430, 860
HhaI	GCGC	4 cut(s)	228, 465, 693, 986
Hin1I	GRCGYC	2 cut(s)	5, 691
Hin1II	CATG	1 cut(s)	982
Hin6I	GCGC	4 cut(s)	226, 463, 691, 984
HinP1I	GCGC	4 cut(s)	226, 463, 691, 984
HincII	GTYRAC	1 cut(s)	159
HindII	GTYRAC	1 cut(s)	159
HindIII	AAGCTT	4 cut(s)	75, 234, 657, 782
HpaII	CCGG	4 cut(s)	183, 308, 430, 860
HphI	GGTGA	2 cut(s)	494, 884
Hpy166II	GTNNAC	3 cut(s)	159, 421, 875
Hpy188III	TCNNGA	6 cut(s)	437, 605, 635, 716, 829, 840
Hpy8I	GTNNAC	3 cut(s)	159, 421, 875
Hpy99I	CGWCG	4 cut(s)	10, 224, 307, 399
HpyAV	CCTTC	6 cut(s)	240, 246, 355, 713, 899, 1027
HpyCH4III	ACNGT	3 cut(s)	170, 1102, 1135
HpyCH4IV	ACGT	1 cut(s)	508
HpyCH4V	TGCA	4 cut(s)	825, 896, 928, 1138
HpyF10VI	GCNNNNNNNGC	3 cut(s)	290, 992, 1144
HpyF3I	CTNAG	2 cut(s)	107, 1043
HpySE526I	ACGT	1 cut(s)	508
Hsp92I	GRCGYC	2 cut(s)	5, 691
Hsp92II	CATG	1 cut(s)	982
HspAI	GCGC	4 cut(s)	226, 463, 691, 984
KasI	GGCGCC	1 cut(s)	690
Kzo9I	GATC	3 cut(s)	607, 712, 1092
LmnI	GCTCC	1 cut(s)	373
Lsp1109I	GCAGC	1 cut(s)	53
LweI	GCATC	3 cut(s)	340, 834, 1134
MabI	ACCWGGT	1 cut(s)	877
MaeII	ACGT	1 cut(s)	508
MaeIII	GTNAC	1 cut(s)	1051
MalI	GATC	3 cut(s)	609, 714, 1094
MboI	GATC	3 cut(s)	607, 712, 1092
MboII	GAAGA	5 cut(s)	15, 38, 391, 813, 855
MfeI	CAATTG	1 cut(s)	294
MflI	RGATCY	2 cut(s)	712, 1092
MhlI	GDGCHC	1 cut(s)	497
MluCI	AATT	9 cut(s)	53, 115, 205, 294, 626, 813, 897, 990, 1016
Mly113I	GGCGCC	1 cut(s)	691
MlyI	GAGTC	3 cut(s)	122, 154, 557
MmeI	TCCRAC	2 cut(s)	377, 865
MnlI	CCTC	6 cut(s)	25, 116, 204, 433, 1015, 1057
MseI	TTAA	4 cut(s)	758, 965, 1119, 1159
MspI	CCGG	4 cut(s)	183, 308, 430, 860
MspR9I	CCNGG	5 cut(s)	184, 430, 538, 860, 879
MunI	CAATTG	1 cut(s)	294
MvaI	CCWGG	2 cut(s)	538, 879
MwoI	GCNNNNNNNGC	3 cut(s)	290, 992, 1144
NarI	GGCGCC	1 cut(s)	691
NciI	CCSGG	3 cut(s)	184, 430, 860
NdeII	GATC	3 cut(s)	607, 712, 1092
NlaIII	CATG	1 cut(s)	982
NlaIV	GGNNCC	1 cut(s)	692
PaeR7I	CTCGAG	1 cut(s)	437
PceI	AGGCCT	1 cut(s)	214
PfeI	GAWTC	7 cut(s)	485, 526, 677, 832, 921, 955, 1058
PkrI	GCNGC	1 cut(s)	68
PleI	GAGTC	3 cut(s)	122, 154, 557
PluTI	GGCGCC	1 cut(s)	694
PpsI	GAGTC	3 cut(s)	122, 154, 557
Psp6I	CCWGG	2 cut(s)	536, 877
PspFI	CCCAGC	1 cut(s)	995
PspGI	CCWGG	2 cut(s)	536, 877
PspN4I	GGNNCC	1 cut(s)	692
PspPI	GGNCC	1 cut(s)	1066
PsuI	RGATCY	2 cut(s)	712, 1092
RsaI	GTAC	3 cut(s)	591, 746, 1041
RsaNI	GTAC	3 cut(s)	590, 745, 1040
SaqAI	TTAA	4 cut(s)	758, 965, 1119, 1159
SatI	GCNGC	1 cut(s)	67
Sau3AI	GATC	3 cut(s)	607, 712, 1092
Sau96I	GGNCC	1 cut(s)	1066
ScaI	AGTACT	1 cut(s)	1041
SchI	GAGTC	3 cut(s)	122, 154, 557
ScrFI	CCNGG	5 cut(s)	184, 430, 538, 860, 879
SduI	GDGCHC	1 cut(s)	497
SexAI	ACCWGGT	1 cut(s)	877
SfaNI	GCATC	3 cut(s)	340, 834, 1134
SfcI	CTRYAG	1 cut(s)	672
SfoI	GGCGCC	1 cut(s)	692
Sfr274I	CTCGAG	1 cut(s)	437
SlaI	CTCGAG	1 cut(s)	437
SmlI	CTYRAG	2 cut(s)	437, 603
SmoI	CTYRAG	2 cut(s)	437, 603
Sse9I	AATT	9 cut(s)	53, 115, 205, 294, 626, 813, 897, 990, 1016
SseBI	AGGCCT	1 cut(s)	214
SsiI	CCGC	2 cut(s)	91, 491
SspDI	GGCGCC	1 cut(s)	690
StuI	AGGCCT	1 cut(s)	214
StyD4I	CCNGG	5 cut(s)	182, 428, 536, 858, 877
StyI	CCWWGG	2 cut(s)	408, 651
TaaI	ACNGT	3 cut(s)	170, 1102, 1135
TaiI	ACGT	1 cut(s)	511
TaqI	TCGA	4 cut(s)	209, 438, 579, 1086
TasI	AATT	9 cut(s)	53, 115, 205, 294, 626, 813, 897, 990, 1016
TatI	WGTACW	1 cut(s)	1039
TfiI	GAWTC	7 cut(s)	485, 526, 677, 832, 921, 955, 1058
Tru1I	TTAA	4 cut(s)	758, 965, 1119, 1159
Tru9I	TTAA	4 cut(s)	758, 965, 1119, 1159
TscAI	CASTG	1 cut(s)	451
TseI	GCWGC	1 cut(s)	66
TspDTI	ATGAA	5 cut(s)	32, 133, 744, 825, 968
TspGWI	ACGGA	2 cut(s)	70, 762
TspRI	CASTG	1 cut(s)	451
XapI	RAATTY	3 cut(s)	205, 813, 1016
XcmI	CCANNNNNNNNNTGG	2 cut(s)	415, 1103
XhoI	CTCGAG	1 cut(s)	437
ZrmI	AGTACT	1 cut(s)	1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.