RchiOBHm_Chr2g0166481

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009881
Physical Location & Seq
Forward (+)
81126151 .. 81132110
5960 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53436

Sequence Viewer

Length: 2952 bp
ATGACCTCGTCGACCACCTCGTTCGCTGCTTCCGGCGTCGGCCTTCGTCTTCCCCAGTCTGTTTCCACCAAGTGTTGCAGTAAAGCTTCTCTCTTTCCTCGCCCAACTCTCTCCCTCACCTTCCACGCCAGACCCCAATTCTTCCGGGGACTAGCGTCCCGGCAATTCAACCCGAACGGTGCGTTTGGGACCGGGTCGGGCCGACTGGGCCGGGCTCCGAACCCGTTTGTTGTGCGCTCCGAGGCTTCTTCAACCGGAAGGATTACACAGCAGGAATTCACTGAAATGGCGTGGCAGGCGGTGGTTTCGTCGCCGGAAGTGGCCAAGGAGAACAAGCATCAGATAGTGGAGACTGAGCATTTGATGAAGGCATTGCTTGAGCAGAAGAATGGGCTTGCTCGTCGGATTTTCGCTAAGGCTGGGGTGGATAATAGTAGGCTTCTTGAGGCCACTGATAAGTATATTCAGCGCCAACCTAAGGTTCTTGGTGACTCTGCGGGATCGATGTTAGGACGTGATTTGGAAGCCTTGCTTCAGAGAGCCAGGGATTACAAGAAGGAATATGGGGACTCGTTTGTGTCTGTGGAGCATTTGGTTCTTGGTTTCATTCAAGATCAACGATTTGGGAAGCAAATATTTAAGGATTTTCAAATCTCCAAGCAGTCTTTGAAGTCGGCTATAGAATCCATAAGGGGACGGCAATCAGTTATTGACCAAGATCCTGAGGGGAAATATGAGTCCCTTGAAAAGTATGGAAAAGATCTGACAGCCATGGCAAAAGAAGGAAAGCTTGACCCGGTAATAGGAAGAGATGATGAAATACGCAGGTGCATCCAGATTCTCTCAAGAAGAACAAAGAATAACCCAGTGCTGATTGGTGAACCAGGTGTTGGGAAAACTGCGATTTCAGAAGGGCTTGCTCAGCGAATTGTGCAAGGGGATGTCCCACAAGCTTTAATGAATCGTAAGCTAATATCTCTTGACATGGGTGCACTAATTGCTGGAGCAAAATATCGGGGAGAGTTTGAGGATAGGCTGAAGGCTGTACTCAAGGAAGTAACAGAATCAGAGGGCCAGATTGTCCTTTTTATTGATGAGATCCACACAGTTGTCGGGGCAGGTGCTACAAATGGTGCAATGGATGCTGGCAATCTTTTAAAACCCATGCTTGGTCGGGGAGAGTTGCGGTGTATAGGTGCAACAACACTGGATGAATATCGCAAATATATTGAGAAAGATCCAGCATTGGAGCGTCGTTTTCAGCAAGTTTATGTTGATCAACCTACTGTTGAGGATACAATTTCGATACTCCGGGGATTGCGTGAAAGATATGAGTTGCATCACGGGGTCCGCATTTCTGATGGTGCACTTGTGGAAGCTGCAATTCTCTCAGACCGTTACATCAGTGGGAGATTTTTACCTGACAAAGCTATTGACTTAGTTGATGAAGCTGCTGCTAAATTGAAAATGGAAATTACATCAAAGCCCACAGCCCTAGATGAGATCAATCGTTCAGTATTGAAACTTGAAATGGAAAGACTGTCACTTACAAATGACACTGACAAAGCGTCCAAAGAGAGATTGAACCGCCTTGAGGCAGAGTTGTCCATCTTAAAAGAGAAACAATCTCAGCTGTCTGAGCAGTGGGAACATGAGAAGTCTGTCATGACTCGCATTCAGTCAATCAAGGAAGAGGTTGACAGGGTAAATCTGGAGATCCAGCAGGCTGAACGAGAGTATGATCTTAACCGTGCTGCTGAGCTGAAGTATGGAAGTCTGAATTCCTTACAGCGCCAACTCCTTGGTGCTGAAAAGGAGCTCGATGAGTATATCAGGTCTGGGAAATCAATGCTGAGGGAGGAAGTTACGGGAAATGACATTTCTGAAATTGTCAGCAAGTGGACTGGTATTCCTGTTTCCAAGCTTCTACAGTCAGAGAGGGAAAAGCTCTTGCATTTGGAGGAAGAGCTGCATAAACGTGTTGTAGGACAGGATCCTGCAGTGAAATCAGTAGCTGAAGCTATTCAGCGGTCTCGAGCAGGTCTCTCAGATCCACATCGCCCGATCGCTAGTTTCATGTTCATGGGCCCCACCGGTGTAGGGAAGACAGAACTAGCCAAGACCCTTGCTTCCTACATGTTCAACACTGAAGAAGCTCTTGTTAGAATTGATATGAGTGAGTACATGGAAAAGCATGCTGTCTCAAGACTGATAGGAGCTCCACCTGGCTATGTGGGGTATGAGGAGGGAGGACAGCTGACGGAGGTTGTTCGCCGGAGGCCATATTCAGTTATCCTATTTGACGAGATTGAAAAGGCGCATTCTGATGTGTTCAATGTTTTCCTTCAAATTTTAGATGATGGGAGAGTAACTGACTCACAGGGCCGCACTGTGAGTTTCACCAACACCGTGATCATTATGACCTCAAATGTTGGTTCACAGTACATACTTAATGGAGATGATGAAGTATCGCCAAAGGAGTTAGGTTATGAAACTATAAAGCAGCGGGTCATGGAGGCTGCAAGGTCCATATTTCGCCCTGAGTTCATGAATCGGGTTGATGAGTACATAGTTTTCCAGCCCTTGGACCGTGATCAGATAAACAACATTGTCAAGATACAGTTGCAACGAGTCCAAAAGAGAATTGCAGACCGCAAGATGAAGATCCAAGTGAGCGAGGCAGCTGTGCAGCTTCTTGGAAATCTCGGCTACGATCCAAACTATGGTGCTAGGCCAGTCAAGCGAGTGATTCAGCAATATGTGGAAAATGAGCTTGCCAAGGGCATCTTGAGGGGAGAGTTTAAAGAAGAAGACATCATTTTGGTCGACACAGAGGTCACGGCGTTTGCCAATGGCCAGCTACCCCAGCAAAAGCTAGTCTTCAAGACGGTTGAAACTGGTTCAGAATCTCCTGCAGCGGAGAACAAAGAAGCTTTGTCAGGGGCCGTTTAA

Protein Analysis

983

Amino Acids

110.06

Weight (kDa)

6.26

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 92 - 215 2.3e-35 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 272 - 373 8.6e-06 SMAX1 nucleotide binding domain
AAA PF00004 290 - 421 2e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 429 - 531 7.3e-34 AAA lid domain
Sigma54_activat PF00158 664 - 810 5e-06 Sigma-54 interaction domain
AAA_2 PF07724 687 - 855 8.8e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 692 - 813 2e-11 AAA domain (dynein-related subfamily)
AAA PF00004 693 - 809 1.7e-08 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 862 - 941 2.7e-26 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 816, 1109
AasI GACNNNNNNGTC 1 cut(s) 2834
Acc36I ACCTGC 3 cut(s) 816, 1109, 2030
AccB7I CCANNNNNTGG 3 cut(s) 890, 2584, 2723
AccI GTMKAC 2 cut(s) 11, 2826
AcoI YGGCCR 2 cut(s) 321, 2854
AcsI RAATTY 3 cut(s) 275, 1780, 2349
AcuI CTGAAG 5 cut(s) 518, 1058, 1784, 2037, 2169
AcyI GRCGYC 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 72
AfaI GTAC 4 cut(s) 1047, 2183, 2444, 2567
AfiI CCNNNNNNNGG 8 cut(s) 478, 803, 890, 1169, 1594, 2100, 2584, 2723
AflIII ACRYGT 2 cut(s) 1978, 2136
AgeI ACCGGT 1 cut(s) 2093
AhdI GACNNNNNGTC 1 cut(s) 1567
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 3 cut(s) 542, 883, 2224
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 873, 905
Alw21I GWGCWC 4 cut(s) 994, 1369, 1821, 2221
Alw26I GTCTC 4 cut(s) 344, 2037, 2048, 2206
Alw44I GTGCAC 2 cut(s) 990, 1365
AlwNI CAGNNNCTG 1 cut(s) 2015
Ama87I CYCGRG 1 cut(s) 2034
ApaI GGGCCC 1 cut(s) 2090
ApaLI GTGCAC 2 cut(s) 990, 1365
ApoI RAATTY 3 cut(s) 275, 1780, 2349
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AsiGI ACCGGT 1 cut(s) 2093
Asp700I GAANNNNTTC 1 cut(s) 2022
AspLEI GCGC 4 cut(s) 237, 471, 1794, 2320
AsuC2I CCSGG 6 cut(s) 146, 160, 193, 212, 797, 1313
AsuHPI GGTGA 4 cut(s) 109, 500, 890, 2392
AvaI CYCGRG 1 cut(s) 2034
AvaII GGWCC 4 cut(s) 189, 1348, 2526, 2587
AxyI CCTNAGG 2 cut(s) 477, 723
BaeGI GKGCMC 3 cut(s) 994, 1369, 2090
BalI TGGCCA 2 cut(s) 323, 2856
BamHI GGATCC 1 cut(s) 1993
BanII GRGCYC 4 cut(s) 217, 1821, 2090, 2221
BbsI GAAGAC 4 cut(s) 41, 2111, 2817, 2872
Bbv12I GWGCWC 4 cut(s) 994, 1369, 1821, 2221
BbvCI CCTCAGC 1 cut(s) 1853
BccI CCATC 3 cut(s) 1355, 1616, 2354
BceAI ACGGC 3 cut(s) 713, 2856, 2930
BciT130I CCWGG 3 cut(s) 544, 885, 2226
BciVI GTATCC 1 cut(s) 1288
BclI TGATCA 3 cut(s) 1276, 2412, 2593
BcnI CCSGG 6 cut(s) 146, 160, 193, 212, 797, 1313
BcoDI GTCTC 4 cut(s) 344, 2037, 2048, 2206
BfaI CTAG 6 cut(s) 152, 1496, 2070, 2114, 2730, 2876
BfmI CTRYAG 4 cut(s) 678, 1928, 1998, 2913
BfoI RGCGCY 2 cut(s) 472, 1795
BfuAI ACCTGC 3 cut(s) 816, 1109, 2030
BfuI GTATCC 1 cut(s) 1288
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 760
BlpI GCTNAGC 2 cut(s) 921, 1758
Bme1390I CCNGG 9 cut(s) 146, 160, 193, 212, 544, 797, 885, 1313, 2226
Bme18I GGWCC 4 cut(s) 189, 1348, 2526, 2587
BmeRI GACNNNNNGTC 1 cut(s) 1567
BmeT110I CYCGRG 1 cut(s) 2034
BmgBI CACGTC 1 cut(s) 515
BmiI GGNNCC 7 cut(s) 190, 216, 1349, 1995, 2088, 2089, 2944
BmrFI CCNGG 9 cut(s) 146, 160, 193, 212, 544, 797, 885, 1313, 2226
BmrI ACTGGG 3 cut(s) 49, 215, 860
BmsI GCATC 5 cut(s) 346, 840, 1132, 1348, 2793
BmuI ACTGGG 3 cut(s) 49, 215, 860
BoxI GACNNNNGTC 1 cut(s) 154
BpiI GAAGAC 4 cut(s) 41, 2111, 2817, 2872
BplI GAGNNNNNCTC 2 cut(s) 2028, 2060
BpmI CTGGAG 2 cut(s) 1023, 1733
Bpu10I CCTNAGC 2 cut(s) 414, 1853
Bpu1102I GCTNAGC 2 cut(s) 921, 1758
BpuEI CTTGAG 7 cut(s) 398, 464, 829, 1034, 1613, 2188, 2809
BpuMI CCSGG 6 cut(s) 146, 160, 193, 212, 797, 1313
Bsa29I ATCGAT 1 cut(s) 503
BsaBI GATNNNNATC 3 cut(s) 1215, 2055, 2663
BsaHI GRCGYC 1 cut(s) 36
BsaI GGTCTC 2 cut(s) 2037, 2048
BsaJI CCNNGG 9 cut(s) 145, 240, 324, 543, 771, 1312, 1801, 2583, 2778
BsaWI WCCGGW 2 cut(s) 254, 2093
Bsc4I CCNNNNNNNGG 8 cut(s) 478, 803, 890, 1169, 1594, 2100, 2584, 2723
Bse118I RCCGGY 1 cut(s) 2093
Bse1I ACTGG 7 cut(s) 55, 210, 866, 1212, 1909, 2735, 2902
Bse21I CCTNAGG 2 cut(s) 477, 723
Bse3DI GCAATG 2 cut(s) 371, 1143
Bse8I GATNNNNATC 3 cut(s) 1215, 2055, 2663
BseBI CCWGG 3 cut(s) 544, 885, 2226
BseCI ATCGAT 1 cut(s) 503
BseDI CCNNGG 9 cut(s) 145, 240, 324, 543, 771, 1312, 1801, 2583, 2778
BseGI GGATG 4 cut(s) 831, 946, 1147, 1216
BseJI GATNNNNATC 3 cut(s) 1215, 2055, 2663
BseLI CCNNNNNNNGG 8 cut(s) 478, 803, 890, 1169, 1594, 2100, 2584, 2723
BseMI GCAATG 2 cut(s) 371, 1143
BseNI ACTGG 7 cut(s) 55, 210, 866, 1212, 1909, 2735, 2902
BseRI GAGGAG 1 cut(s) 2258
BseSI GKGCMC 3 cut(s) 994, 1369, 2090
BseYI CCCAGC 2 cut(s) 419, 2865
BsgI GTGCAG 1 cut(s) 2708
Bsh1285I CGRYCG 1 cut(s) 2067
BshTI ACCGGT 1 cut(s) 2093
BshVI ATCGAT 1 cut(s) 503
BsiEI CGRYCG 1 cut(s) 2067
BsiHKAI GWGCWC 4 cut(s) 994, 1369, 1821, 2221
BsiHKCI CYCGRG 1 cut(s) 2034
BslFI GGGAC 7 cut(s) 142, 162, 202, 581, 708, 724, 929
BslI CCNNNNNNNGG 8 cut(s) 478, 803, 890, 1169, 1594, 2100, 2584, 2723
BsmAI GTCTC 4 cut(s) 344, 2037, 2048, 2206
BsmFI GGGAC 7 cut(s) 142, 162, 202, 581, 708, 724, 929
BsmI GAATGC 2 cut(s) 1674, 2320
Bso31I GGTCTC 2 cut(s) 2037, 2048
BsoBI CYCGRG 1 cut(s) 2034
Bsp120I GGGCCC 1 cut(s) 2086
Bsp1286I GDGCHC 6 cut(s) 217, 994, 1369, 1821, 2090, 2221
Bsp1720I GCTNAGC 2 cut(s) 921, 1758
Bsp19I CCATGG 1 cut(s) 771
BspDI ATCGAT 1 cut(s) 503
BspHI TCATGA 2 cut(s) 1665, 2547
BspLI GGNNCC 7 cut(s) 190, 216, 1349, 1995, 2088, 2089, 2944
BspMAI CTGCAG 2 cut(s) 2002, 2917
BspMI ACCTGC 3 cut(s) 816, 1109, 2030
BspQI GCTCTTC 1 cut(s) 1959
BspTNI GGTCTC 2 cut(s) 2037, 2048
BsrDI GCAATG 2 cut(s) 371, 1143
BsrFI RCCGGY 1 cut(s) 2093
BsrI ACTGG 7 cut(s) 55, 210, 866, 1212, 1909, 2735, 2902
BssAI RCCGGY 1 cut(s) 2093
BssECI CCNNGG 9 cut(s) 145, 240, 324, 543, 771, 1312, 1801, 2583, 2778
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 5 cut(s) 324, 771, 1801, 2583, 2778
Bst2UI CCWGG 3 cut(s) 544, 885, 2226
Bst6I CTCTTC 3 cut(s) 802, 1686, 1959
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 998, 1142
BstC8I GCNNGC 8 cut(s) 297, 396, 918, 1147, 1725, 2196, 2775, 2858
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 4 cut(s) 831, 946, 1147, 1216
BstH2I RGCGCY 2 cut(s) 472, 1795
BstHHI GCGC 4 cut(s) 237, 471, 1794, 2320
BstMAI GTCTC 4 cut(s) 344, 2037, 2048, 2206
BstMCI CGRYCG 1 cut(s) 2067
BstMWI GCNNNNNNNGC 9 cut(s) 207, 296, 922, 931, 998, 1142, 1639, 2740, 2866
BstNI CCWGG 3 cut(s) 544, 885, 2226
BstNSI RCATGY 2 cut(s) 2140, 2198
BstPAI GACNNNNGTC 1 cut(s) 154
BstSCI CCNGG 9 cut(s) 144, 158, 191, 210, 542, 795, 883, 1311, 2224
BstSFI CTRYAG 4 cut(s) 678, 1928, 1998, 2913
BstSLI GKGCMC 3 cut(s) 994, 1369, 2090
BstV2I GAAGAC 4 cut(s) 41, 2111, 2817, 2872
BstX2I RGATCY 8 cut(s) 718, 760, 1098, 1237, 1716, 1993, 2050, 2664
BstXI CCANNNNNNTGG 1 cut(s) 1802
BstYI RGATCY 8 cut(s) 718, 760, 1098, 1237, 1716, 1993, 2050, 2664
Bsu15I ATCGAT 1 cut(s) 503
Bsu36I CCTNAGG 2 cut(s) 477, 723
BsuI GTATCC 1 cut(s) 1288
BsuTUI ATCGAT 1 cut(s) 503
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 1 cut(s) 2042
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 4 cut(s) 831, 946, 1147, 1216
BtsI GCAGTG 2 cut(s) 1649, 2007
BveI ACCTGC 3 cut(s) 816, 1109, 2030
Cac8I GCNNGC 8 cut(s) 297, 396, 918, 1147, 1725, 2196, 2775, 2858
CaiI CAGNNNCTG 1 cut(s) 2015
CciI TCATGA 2 cut(s) 1665, 2547
CfoI GCGC 4 cut(s) 237, 471, 1794, 2320
Cfr10I RCCGGY 1 cut(s) 2093
ClaI ATCGAT 1 cut(s) 503
CseI GACGC 4 cut(s) 25, 144, 1241, 1557
CsiI ACCWGGT 1 cut(s) 883
Csp6I GTAC 4 cut(s) 1046, 2182, 2443, 2566
CspAI ACCGGT 1 cut(s) 2093
CviQI GTAC 4 cut(s) 1046, 2182, 2443, 2566
DraI TTTAAA 2 cut(s) 1158, 2803
DraIII CACNNNGTG 1 cut(s) 72
DrdI GACNNNNNNGTC 1 cut(s) 2834
DriI GACNNNNNGTC 1 cut(s) 1567
DseDI GACNNNNNNGTC 1 cut(s) 2834
EaeI YGGCCR 2 cut(s) 321, 2854
Eam1104I CTCTTC 3 cut(s) 802, 1686, 1959
Eam1105I GACNNNNNGTC 1 cut(s) 1567
EarI CTCTTC 3 cut(s) 802, 1686, 1959
Ecl136II GAGCTC 2 cut(s) 1819, 2219
Eco130I CCWWGG 5 cut(s) 324, 771, 1801, 2583, 2778
Eco24I GRGCYC 4 cut(s) 217, 1821, 2090, 2221
Eco31I GGTCTC 2 cut(s) 2037, 2048
Eco47I GGWCC 4 cut(s) 189, 1348, 2526, 2587
Eco53kI GAGCTC 2 cut(s) 1819, 2219
Eco57I CTGAAG 5 cut(s) 518, 1058, 1784, 2037, 2169
Eco81I CCTNAGG 2 cut(s) 477, 723
Eco88I CYCGRG 1 cut(s) 2034
EcoICRI GAGCTC 2 cut(s) 1819, 2219
EcoO109I RGGNCCY 1 cut(s) 2087
EcoRI GAATTC 2 cut(s) 275, 1780
EcoRII CCWGG 3 cut(s) 542, 883, 2224
EcoT14I CCWWGG 5 cut(s) 324, 771, 1801, 2583, 2778
EcoT38I GRGCYC 4 cut(s) 217, 1821, 2090, 2221
ErhI CCWWGG 5 cut(s) 324, 771, 1801, 2583, 2778
FaqI GGGAC 7 cut(s) 142, 162, 202, 581, 708, 724, 929
FauI CCCGC 2 cut(s) 490, 2499
FbaI TGATCA 3 cut(s) 1276, 2412, 2593
FblI GTMKAC 2 cut(s) 11, 2826
FokI GGATG 4 cut(s) 818, 953, 1154, 1223
FriOI GRGCYC 4 cut(s) 217, 1821, 2090, 2221
FspBI CTAG 6 cut(s) 152, 1496, 2070, 2114, 2730, 2876
GlaI GCGC 4 cut(s) 236, 470, 1793, 2319
GsaI CCCAGC 2 cut(s) 423, 2869
GsuI CTGGAG 2 cut(s) 1023, 1733
HaeII RGCGCY 2 cut(s) 472, 1795
HgaI GACGC 4 cut(s) 25, 144, 1241, 1557
HhaI GCGC 4 cut(s) 237, 471, 1794, 2320
Hin1I GRCGYC 1 cut(s) 36
Hin6I GCGC 4 cut(s) 235, 469, 1792, 2318
HinP1I GCGC 4 cut(s) 235, 469, 1792, 2318
HincII GTYRAC 3 cut(s) 12, 1699, 2827
HindII GTYRAC 3 cut(s) 12, 1699, 2827
HindIII AAGCTT 5 cut(s) 84, 788, 951, 1922, 2931
HphI GGTGA 4 cut(s) 109, 500, 890, 2392
Hpy166II GTNNAC 8 cut(s) 12, 881, 992, 1367, 1699, 1902, 2438, 2827
Hpy8I GTNNAC 8 cut(s) 12, 881, 992, 1367, 1699, 1902, 2438, 2827
Hpy99I CGWCG 5 cut(s) 13, 41, 313, 405, 1257
HpyAV CCTTC 9 cut(s) 53, 130, 252, 361, 550, 776, 905, 1033, 2354
HpyCH4IV ACGT 2 cut(s) 514, 1978
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 296, 922, 931, 998, 1142, 1639, 2740, 2866
HpySE526I ACGT 2 cut(s) 514, 1978
Hsp92I GRCGYC 1 cut(s) 36
HspAI GCGC 4 cut(s) 235, 469, 1792, 2318
Ksp22I TGATCA 3 cut(s) 1276, 2412, 2593
LguI GCTCTTC 1 cut(s) 1959
LmnI GCTCC 8 cut(s) 220, 242, 586, 1004, 1249, 1816, 2216, 2224
LweI GCATC 5 cut(s) 346, 840, 1132, 1348, 2793
MabI ACCWGGT 1 cut(s) 883
MaeI CTAG 6 cut(s) 152, 1496, 2070, 2114, 2730, 2876
MaeII ACGT 2 cut(s) 514, 1978
MaeIII GTNAC 7 cut(s) 488, 1057, 1397, 1542, 1864, 2368, 2836
MflI RGATCY 8 cut(s) 718, 760, 1098, 1237, 1716, 1993, 2050, 2664
MhlI GDGCHC 6 cut(s) 217, 994, 1369, 1821, 2090, 2221
MlsI TGGCCA 2 cut(s) 323, 2856
MluNI TGGCCA 2 cut(s) 323, 2856
MlyI GAGTC 6 cut(s) 485, 563, 746, 1663, 2369, 2640
MmeI TCCRAC 1 cut(s) 383
Mox20I TGGCCA 2 cut(s) 323, 2856
MroXI GAANNNNTTC 1 cut(s) 2022
MscI TGGCCA 2 cut(s) 323, 2856
MseI TTAA 8 cut(s) 639, 956, 1157, 1613, 1746, 2451, 2802, 2950
MslI CAYNNNNRTG 4 cut(s) 284, 1977, 2081, 2325
Msp20I TGGCCA 2 cut(s) 323, 2856
MspA1I CMGCKG 6 cut(s) 1633, 2029, 2257, 2506, 2684, 2918
MspR9I CCNGG 9 cut(s) 146, 160, 193, 212, 544, 797, 885, 1313, 2226
Mva1269I GAATGC 2 cut(s) 1674, 2320
MvaI CCWGG 3 cut(s) 544, 885, 2226
MwoI GCNNNNNNNGC 9 cut(s) 207, 296, 922, 931, 998, 1142, 1639, 2740, 2866
NciI CCSGG 6 cut(s) 146, 160, 193, 212, 797, 1313
NcoI CCATGG 1 cut(s) 771
NlaIV GGNNCC 7 cut(s) 190, 216, 1349, 1995, 2088, 2089, 2944
NmeAIII GCCGAG 1 cut(s) 2685
NmuCI GTSAC 3 cut(s) 488, 1542, 2836
NspI RCATGY 2 cut(s) 2140, 2198
PaeI GCATGC 1 cut(s) 2198
PaeR7I CTCGAG 1 cut(s) 2034
PagI TCATGA 2 cut(s) 1665, 2547
PaqCI CACCTGC 2 cut(s) 816, 1109
PciI ACATGT 1 cut(s) 2136
PciSI GCTCTTC 1 cut(s) 1959
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 2 cut(s) 1674, 2320
PdmI GAANNNNTTC 1 cut(s) 2022
PfeI GAWTC 7 cut(s) 683, 838, 961, 1064, 2551, 2749, 2906
PflFI GACNNNGTC 2 cut(s) 7, 193
PflMI CCANNNNNTGG 3 cut(s) 890, 2584, 2723
PinAI ACCGGT 1 cut(s) 2093
Ple19I CGATCG 1 cut(s) 2067
PleI GAGTC 6 cut(s) 485, 563, 745, 1663, 2369, 2639
PpsI GAGTC 6 cut(s) 485, 563, 745, 1663, 2369, 2639
PscI ACATGT 1 cut(s) 2136
PshAI GACNNNNGTC 1 cut(s) 154
Psp124BI GAGCTC 2 cut(s) 1821, 2221
Psp6I CCWGG 3 cut(s) 542, 883, 2224
PspFI CCCAGC 2 cut(s) 419, 2865
PspGI CCWGG 3 cut(s) 542, 883, 2224
PspN4I GGNNCC 7 cut(s) 190, 216, 1349, 1995, 2088, 2089, 2944
PspOMI GGGCCC 1 cut(s) 2086
PstI CTGCAG 2 cut(s) 2002, 2917
PstNI CAGNNNCTG 1 cut(s) 2015
PsuI RGATCY 8 cut(s) 718, 760, 1098, 1237, 1716, 1993, 2050, 2664
PsyI GACNNNGTC 2 cut(s) 7, 193
PvuI CGATCG 1 cut(s) 2067
PvuII CAGCTG 3 cut(s) 1633, 2257, 2684
RsaI GTAC 4 cut(s) 1047, 2183, 2444, 2567
RsaNI GTAC 4 cut(s) 1046, 2182, 2443, 2566
RseI CAYNNNNRTG 4 cut(s) 284, 1977, 2081, 2325
SacI GAGCTC 2 cut(s) 1821, 2221
SalI GTCGAC 2 cut(s) 10, 2825
SapI GCTCTTC 1 cut(s) 1959
SaqAI TTAA 8 cut(s) 639, 956, 1157, 1613, 1746, 2451, 2802, 2950
SchI GAGTC 6 cut(s) 485, 563, 746, 1663, 2369, 2640
ScrFI CCNGG 9 cut(s) 146, 160, 193, 212, 544, 797, 885, 1313, 2226
SduI GDGCHC 6 cut(s) 217, 994, 1369, 1821, 2090, 2221
SexAI ACCWGGT 1 cut(s) 883
SfaNI GCATC 5 cut(s) 346, 840, 1132, 1348, 2793
SfcI CTRYAG 4 cut(s) 678, 1928, 1998, 2913
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 2034
SgrAI CRCCGGYG 1 cut(s) 2093
SinI GGWCC 4 cut(s) 189, 1348, 2526, 2587
SlaI CTCGAG 1 cut(s) 2034
SmiMI CAYNNNNRTG 4 cut(s) 284, 1977, 2081, 2325
SmlI CTYRAG 8 cut(s) 377, 443, 844, 1049, 1592, 2034, 2203, 2788
SmoI CTYRAG 8 cut(s) 377, 443, 844, 1049, 1592, 2034, 2203, 2788
SphI GCATGC 1 cut(s) 2198
SspI AATATT 1 cut(s) 636
SspMI CTAG 6 cut(s) 152, 1496, 2070, 2114, 2730, 2876
SstI GAGCTC 2 cut(s) 1821, 2221
StyD4I CCNGG 9 cut(s) 144, 158, 191, 210, 542, 795, 883, 1311, 2224
StyI CCWWGG 5 cut(s) 324, 771, 1801, 2583, 2778
TaiI ACGT 2 cut(s) 517, 1981
TaqI TCGA 6 cut(s) 11, 503, 1304, 1821, 2035, 2826
TatI WGTACW 4 cut(s) 1045, 2181, 2442, 2565
TauI GCSGC 1 cut(s) 2388
TfiI GAWTC 7 cut(s) 683, 838, 961, 1064, 2551, 2749, 2906
Tru1I TTAA 8 cut(s) 639, 956, 1157, 1613, 1746, 2451, 2802, 2950
Tru9I TTAA 8 cut(s) 639, 956, 1157, 1613, 1746, 2451, 2802, 2950
TseFI GTSAC 3 cut(s) 488, 1542, 2836
Tsp45I GTSAC 3 cut(s) 488, 1542, 2836
TspGWI ACGGA 1 cut(s) 2276
Tth111I GACNNNGTC 2 cut(s) 7, 193
Van91I CCANNNNNTGG 3 cut(s) 890, 2584, 2723
VneI GTGCAC 2 cut(s) 990, 1365
VpaK11BI GGWCC 4 cut(s) 189, 1348, 2526, 2587
XapI RAATTY 3 cut(s) 275, 1780, 2349
XceI RCATGY 2 cut(s) 2140, 2198
XhoI CTCGAG 1 cut(s) 2034
XmiI GTMKAC 2 cut(s) 11, 2826
XmnI GAANNNNTTC 1 cut(s) 2022
XspI CTAG 6 cut(s) 152, 1496, 2070, 2114, 2730, 2876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.