Rroxscaffold_2G00084860

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009881
Physical Location & Seq
Reverse (-)
7314504 .. 7320107
5604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084860.1

Sequence Viewer

Length: 2958 bp
ATGGCCTCGTCGACCACCTCGTTCGCTGCTTCCGGCGTCGGTCTTCGTCTTCCCCAGTCTGTTTCCACCAAATGTTGCAGTAAATCTTCTCTCTCTTCCTCACCCAACTCTCTCCTCACCTTTCACGCCAGGCCCCAATTCTTCCGGGGACTAGCTTCCCGGCAATTCAACCCCAACGGTGCGTTTGGAACCGGGTCGGGCAGACTGGGCCGGACTCCGAACCCGTTTGTTGTGCGCTCCGAGGCTTCTTCAACCGGAAGGGGACAGATTACACAGCAGGAATTCACTGAAATGGCGTGGCAGGCGGTGGTTTCGTCGCCGGAAGTGGCCAAGGAGAACAAGCATCAGATAGTGGAGACTGAGCATTTGATGAAGGCATTGCTTGAGCAGAAGAATGGGCTTGCTCGTCGGATTTTCGCTAAGGCTGGGGTGGATAATAGTAGGCTTCTTGAGGCCACTGATAAGTATATTCAGCGCCAACCTAAGGTTCTTGGTGACTCTGCGGGATCGATGTTAGGACGTGATTTGGAAGCCTTGCTTCAGAGAGCCAGGGATTACAAGAAGGAATATGGGGACTCGTTTGTGTCTGTGGAGCATTTGGTTCTTGGTTTCATTCAAGATCAACGATTTGGGAAGCAAATATTTAAGGATTTTCAAATCTCCAAGCAGTCTTTGAAGTCAGCTATAGAATCCATAAGGGGACGCCAATCAGTTATTGACCAAGATCCTGAGGGGAAATATGAGTCCCTTGAAAAGTATGGAAAAGATCTGACAGCCATGGCAAAAGAAGGAAAGCTGGACCCGGTAATAGGAAGAGATGATGAAATACGCAGGTGCATCCAGATTCTCTCAAGAAGAACAAAGAACAACCCAGTGCTGATTGGTGAACCAGGTGTTGGGAAAACTGCAATTTCAGAAGGGCTTGCTCAGCGAATTGTGCAAGGGGATGTCCCACAAGCTTTAATGAATCGTAAGCTAATATCTCTTGACATGGGTGCGCTAATTGCTGGAGCAAAATATCGGGGAGAGTTTGAGGATAGGCTGAAGGCTGTACTCAAGGAAGTAACAGAATCAGAGGGCCAGATTATCCTTTTTATTGATGAGATCCACACAGTTGTTGGGGCAGGTGCTACAAATGGTGCAATGGATGCTGGCAATCTATTAAAACCCATGCTTGGTCGGGGAGAGTTGCGGTGTATAGGTGCAACAACACTGGATGAATATCGCAAGTATATTGAGAAAGATCCAGCATTGGAGCGTCGTTTTCAGCAAGTTTATGTTGATCAACCTACTGTTGAGGATACAATTTCGATACTCCGAGGATTGCGTGAAAGATATGAGTTGCATCACGGGGTCCGCATTTCTGATGGTGCACTTGTGGAAGCTGCAATTCTCTCAGACCGTTACATCAGTGGGAGATTTTTACCTGACAAAGCTATCGACCTAGTTGATGAAGCTGCTGCCAAGTTGAAAATGGAAATTACATCAAAGCCCACAGCCCTAGATGAGATCAATCGTTCAGTATTGAAACTTGAAATGGAAAGACTGTCACTTACAAATGACACCGACAAAGCGTCCAAAGAGAGATTGAACCGCCTTGAGGCAGAGTTGTCCATCTTAAAAGAGAAACAATCTCAGCTGTCTGAGCAGTGGGAACATGAGAAGTCTGTCATGACTCGCATTCAGTCGATCAAGGAAGAGGTTGACAGGGTAAATCTGGAGATCCAGCAGGCTGAACGAGAGTATGATCTTAATCGTGCTGCTGAGCTGAAGTATGGGAGTCTGAATTCCTTACAGCGCCAACTCCTCAGTGCTGAAAAGGAGCTCGATGAGTATATCAGGTCTGGGAAATCAATGCTGAGAGAGGAAGTTACTGGAAATGACATTTCTGAAATTGTCAGCAAGTGGACTGGTATTCCTGTTTCCAAGCTTCTACAGTCAGAGAGGGAAAAACTCTTGCATTTGGAGGAAGAACTGCATAAACGTGTTGTAGGACAGGATCCTGCAGTGAAATCAGTAGCTGAAGCTATTCAGCGGTCTCGAGCAGGTCTCTCAGATCCTCATCGCCCAATCGCTAGTTTCATGTTCATGGGCCCCACCGGTGTAGGGAAGACAGAACTAGCCAAGACCCTTGCTTCCTACATGTTCAACACTGAAGAAGCTCTTGTTAGAATTGATATGAGTGAGTACATGGAAAAGCATGCTGTCTCAAGACTAATAGGTGCTCCACCAGGCTATGTGGGGTATGAGGAGGGAGGACAGCTGACAGAGGTTGTTCGCCGGAGGCCATATTCAGTTATCCTGTTTGACGAGATTGAAAAGGCACATTCTGATGTGTTCAATGTTTTCCTTCAAATTTTAGATGATGGGAGAGTAACTGACTCACAGGGCCGCACTGTGAGTTTCACCAACACTGTGATCATTATGACCTCAAATGTTGGTTCACAGTACATACTTAACGGAGATGATGAAGTATCGCCAAAGGAGTTGGGTTATGAAACTATAAAGCAGCGGGTCATGGAGGCTGCAAGGTCCATATTTCGCCCTGAGTTCATGAATCGGGTTGATGAGTACATAGTTTTCCAGCCCTTGGACCGTGATCAGATAAACAACATTGTCAAGATACAGTTGGAACGAGTCCAAAAGAGAATTGCAGACCGCAAGATGAAGATCCAAGTGAGCGAGGCAGCTGTGCAGCTTCTTGGAAATCTCGGCTACGATCCAAACTATGGTGCTAGGCCAGTCAAGCGAGTGATTCAGCAATATGTAGAAAATGAGCTTGCCAAGGGCATCTTGAGAGGAGAGTTCAAAGAAGAAGACACCATTTTGGTCGACACAGAGGTCACGGCGTTTGCCAATGGCCAGCTACCCCAGCAAAAGTTATTCTTCAAGACGCTTGAAACTGGTTCAGAATCTGCTGCAACAAAGAACAAAGAAGCTTTGTCAGGGGCCGTGTAA

Protein Analysis

985

Amino Acids

110.24

Weight (kDa)

6.26

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 94 - 217 2.3e-35 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 274 - 375 6.4e-06 SMAX1 nucleotide binding domain
AAA PF00004 292 - 423 1.9e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 431 - 533 7.3e-34 AAA lid domain
Sigma54_activat PF00158 666 - 812 5.1e-06 Sigma-54 interaction domain
AAA_2 PF07724 689 - 857 8.9e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 694 - 815 2e-11 AAA domain (dynein-related subfamily)
AAA PF00004 695 - 811 1.7e-08 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 864 - 944 7.4e-27 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 822, 1115
AasI GACNNNNNNGTC 1 cut(s) 2840
Acc36I ACCTGC 3 cut(s) 822, 1115, 2036
AccB7I CCANNNNNTGG 3 cut(s) 896, 2590, 2729
AccI GTMKAC 2 cut(s) 11, 2832
AciI CCGC 9 cut(s) 305, 503, 1192, 1357, 1594, 2035, 2392, 2512, 2659
AcoI YGGCCR 2 cut(s) 327, 2860
AcsI RAATTY 3 cut(s) 281, 1786, 2355
AcuI CTGAAG 5 cut(s) 524, 1064, 1790, 2043, 2175
AcyI GRCGYC 2 cut(s) 36, 703
AfaI GTAC 4 cut(s) 1053, 2189, 2450, 2573
AfiI CCNNNNNNNGG 8 cut(s) 484, 809, 896, 1175, 1600, 2106, 2590, 2729
AflIII ACRYGT 2 cut(s) 1984, 2142
AgeI ACCGGT 1 cut(s) 2099
AhdI GACNNNNNGTC 1 cut(s) 1573
AjiI CACGTC 1 cut(s) 521
AjnI CCWGG 4 cut(s) 128, 548, 889, 2230
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 879, 911
Alw21I GWGCWC 3 cut(s) 1375, 1827, 2227
Alw26I GTCTC 4 cut(s) 350, 2043, 2054, 2212
Alw44I GTGCAC 1 cut(s) 1371
AlwNI CAGNNNCTG 2 cut(s) 2021, 2915
Ama87I CYCGRG 1 cut(s) 2040
ApaI GGGCCC 1 cut(s) 2096
ApaLI GTGCAC 1 cut(s) 1371
ApoI RAATTY 3 cut(s) 281, 1786, 2355
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
AsiGI ACCGGT 1 cut(s) 2099
Asp700I GAANNNNTTC 1 cut(s) 2028
AspLEI GCGC 4 cut(s) 237, 477, 1000, 1800
AsuC2I CCSGG 4 cut(s) 146, 160, 193, 803
AsuHPI GGTGA 5 cut(s) 93, 109, 506, 896, 2398
AvaI CYCGRG 1 cut(s) 2040
AvaII GGWCC 4 cut(s) 799, 1354, 2532, 2593
AxyI CCTNAGG 2 cut(s) 483, 729
BaeGI GKGCMC 2 cut(s) 1375, 2096
BalI TGGCCA 2 cut(s) 329, 2862
BamHI GGATCC 1 cut(s) 1999
BanII GRGCYC 2 cut(s) 1827, 2096
BbsI GAAGAC 4 cut(s) 35, 41, 2117, 2823
Bbv12I GWGCWC 3 cut(s) 1375, 1827, 2227
BccI CCATC 3 cut(s) 1361, 1622, 2360
BceAI ACGGC 2 cut(s) 2862, 2936
BciT130I CCWGG 4 cut(s) 130, 550, 891, 2232
BciVI GTATCC 1 cut(s) 1294
BclI TGATCA 3 cut(s) 1282, 2418, 2599
BcnI CCSGG 4 cut(s) 146, 160, 193, 803
BcoDI GTCTC 4 cut(s) 350, 2043, 2054, 2212
BfaI CTAG 6 cut(s) 152, 1445, 1502, 2076, 2120, 2736
BfmI CTRYAG 3 cut(s) 684, 1934, 2004
BfoI RGCGCY 2 cut(s) 478, 1801
BfuAI ACCTGC 3 cut(s) 822, 1115, 2036
BfuI GTATCC 1 cut(s) 1294
BglII AGATCT 1 cut(s) 766
BlpI GCTNAGC 2 cut(s) 927, 1764
Bme1390I CCNGG 8 cut(s) 130, 146, 160, 193, 550, 803, 891, 2232
Bme18I GGWCC 4 cut(s) 799, 1354, 2532, 2593
BmeRI GACNNNNNGTC 1 cut(s) 1573
BmeT110I CYCGRG 1 cut(s) 2040
BmgBI CACGTC 1 cut(s) 521
BmiI GGNNCC 8 cut(s) 134, 190, 801, 1355, 2001, 2094, 2095, 2950
BmrFI CCNGG 8 cut(s) 130, 146, 160, 193, 550, 803, 891, 2232
BmrI ACTGGG 3 cut(s) 49, 215, 866
BmsI GCATC 5 cut(s) 352, 846, 1138, 1354, 2799
BmuI ACTGGG 3 cut(s) 49, 215, 866
BpiI GAAGAC 4 cut(s) 35, 41, 2117, 2823
BplI GAGNNNNNCTC 2 cut(s) 2034, 2066
BpmI CTGGAG 2 cut(s) 1029, 1739
Bpu10I CCTNAGC 1 cut(s) 420
Bpu1102I GCTNAGC 2 cut(s) 927, 1764
BpuEI CTTGAG 7 cut(s) 404, 470, 835, 1040, 1619, 2194, 2815
BpuMI CCSGG 4 cut(s) 146, 160, 193, 803
Bsa29I ATCGAT 1 cut(s) 509
BsaBI GATNNNNATC 4 cut(s) 1221, 1752, 2061, 2669
BsaHI GRCGYC 2 cut(s) 36, 703
BsaI GGTCTC 2 cut(s) 2043, 2054
BsaJI CCNNGG 8 cut(s) 145, 240, 330, 549, 777, 1318, 2589, 2784
BsaWI WCCGGW 2 cut(s) 254, 2099
Bsc4I CCNNNNNNNGG 8 cut(s) 484, 809, 896, 1175, 1600, 2106, 2590, 2729
Bse118I RCCGGY 1 cut(s) 2099
Bse1I ACTGG 8 cut(s) 55, 210, 872, 1218, 1879, 1915, 2741, 2908
Bse21I CCTNAGG 2 cut(s) 483, 729
Bse3DI GCAATG 2 cut(s) 377, 1149
Bse8I GATNNNNATC 4 cut(s) 1221, 1752, 2061, 2669
BseBI CCWGG 4 cut(s) 130, 550, 891, 2232
BseCI ATCGAT 1 cut(s) 509
BseDI CCNNGG 8 cut(s) 145, 240, 330, 549, 777, 1318, 2589, 2784
BseGI GGATG 4 cut(s) 837, 952, 1153, 1222
BseJI GATNNNNATC 4 cut(s) 1221, 1752, 2061, 2669
BseLI CCNNNNNNNGG 8 cut(s) 484, 809, 896, 1175, 1600, 2106, 2590, 2729
BseMI GCAATG 2 cut(s) 377, 1149
BseNI ACTGG 8 cut(s) 55, 210, 872, 1218, 1879, 1915, 2741, 2908
BseRI GAGGAG 4 cut(s) 104, 1796, 2264, 2814
BseSI GKGCMC 2 cut(s) 1375, 2096
BseYI CCCAGC 2 cut(s) 425, 2871
BsgI GTGCAG 1 cut(s) 2714
BshTI ACCGGT 1 cut(s) 2099
BshVI ATCGAT 1 cut(s) 509
BsiHKAI GWGCWC 3 cut(s) 1375, 1827, 2227
BsiHKCI CYCGRG 1 cut(s) 2040
BslFI GGGAC 6 cut(s) 162, 276, 587, 714, 730, 935
BslI CCNNNNNNNGG 8 cut(s) 484, 809, 896, 1175, 1600, 2106, 2590, 2729
BsmAI GTCTC 4 cut(s) 350, 2043, 2054, 2212
BsmFI GGGAC 6 cut(s) 162, 276, 587, 714, 730, 935
BsmI GAATGC 1 cut(s) 1680
Bso31I GGTCTC 2 cut(s) 2043, 2054
BsoBI CYCGRG 1 cut(s) 2040
Bsp120I GGGCCC 1 cut(s) 2092
Bsp1286I GDGCHC 4 cut(s) 1375, 1827, 2096, 2227
Bsp1720I GCTNAGC 2 cut(s) 927, 1764
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 9 cut(s) 305, 503, 1192, 1357, 1594, 2035, 2392, 2512, 2659
BspDI ATCGAT 1 cut(s) 509
BspHI TCATGA 2 cut(s) 1671, 2553
BspLI GGNNCC 8 cut(s) 134, 190, 801, 1355, 2001, 2094, 2095, 2950
BspMAI CTGCAG 1 cut(s) 2008
BspMI ACCTGC 3 cut(s) 822, 1115, 2036
BspTNI GGTCTC 2 cut(s) 2043, 2054
BsrDI GCAATG 2 cut(s) 377, 1149
BsrFI RCCGGY 1 cut(s) 2099
BsrI ACTGG 8 cut(s) 55, 210, 872, 1218, 1879, 1915, 2741, 2908
BssAI RCCGGY 1 cut(s) 2099
BssECI CCNNGG 8 cut(s) 145, 240, 330, 549, 777, 1318, 2589, 2784
BssNI GRCGYC 2 cut(s) 36, 703
BssT1I CCWWGG 4 cut(s) 330, 777, 2589, 2784
Bst2UI CCWGG 4 cut(s) 130, 550, 891, 2232
Bst6I CTCTTC 3 cut(s) 100, 808, 1692
BstACI GRCGYC 2 cut(s) 36, 703
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstC8I GCNNGC 8 cut(s) 303, 402, 924, 1153, 1731, 2202, 2781, 2864
BstDSI CCRYGG 1 cut(s) 777
BstF5I GGATG 4 cut(s) 837, 952, 1153, 1222
BstH2I RGCGCY 2 cut(s) 478, 1801
BstHHI GCGC 4 cut(s) 237, 477, 1000, 1800
BstMAI GTCTC 4 cut(s) 350, 2043, 2054, 2212
BstMWI GCNNNNNNNGC 9 cut(s) 207, 302, 928, 937, 1004, 1148, 1645, 2746, 2872
BstNI CCWGG 4 cut(s) 130, 550, 891, 2232
BstNSI RCATGY 2 cut(s) 2146, 2204
BstSCI CCNGG 8 cut(s) 128, 144, 158, 191, 548, 801, 889, 2230
BstSFI CTRYAG 3 cut(s) 684, 1934, 2004
BstSLI GKGCMC 2 cut(s) 1375, 2096
BstV2I GAAGAC 4 cut(s) 35, 41, 2117, 2823
BstX2I RGATCY 8 cut(s) 724, 766, 1104, 1243, 1722, 1999, 2056, 2670
BstYI RGATCY 8 cut(s) 724, 766, 1104, 1243, 1722, 1999, 2056, 2670
Bsu15I ATCGAT 1 cut(s) 509
Bsu36I CCTNAGG 2 cut(s) 483, 729
BsuI GTATCC 1 cut(s) 1294
BsuTUI ATCGAT 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 777
BtgZI GCGATG 1 cut(s) 2048
BtrI CACGTC 1 cut(s) 521
BtsCI GGATG 4 cut(s) 837, 952, 1153, 1222
BtsI GCAGTG 2 cut(s) 1655, 2013
BveI ACCTGC 3 cut(s) 822, 1115, 2036
Cac8I GCNNGC 8 cut(s) 303, 402, 924, 1153, 1731, 2202, 2781, 2864
CaiI CAGNNNCTG 2 cut(s) 2021, 2915
CciI TCATGA 2 cut(s) 1671, 2553
CfoI GCGC 4 cut(s) 237, 477, 1000, 1800
Cfr10I RCCGGY 1 cut(s) 2099
ClaI ATCGAT 1 cut(s) 509
CseI GACGC 5 cut(s) 25, 711, 1247, 1563, 2902
CsiI ACCWGGT 1 cut(s) 889
Csp6I GTAC 4 cut(s) 1052, 2188, 2449, 2572
CspAI ACCGGT 1 cut(s) 2099
CviQI GTAC 4 cut(s) 1052, 2188, 2449, 2572
DrdI GACNNNNNNGTC 1 cut(s) 2840
DriI GACNNNNNGTC 1 cut(s) 1573
DseDI GACNNNNNNGTC 1 cut(s) 2840
EaeI YGGCCR 2 cut(s) 327, 2860
Eam1104I CTCTTC 3 cut(s) 100, 808, 1692
Eam1105I GACNNNNNGTC 1 cut(s) 1573
EarI CTCTTC 3 cut(s) 100, 808, 1692
Ecl136II GAGCTC 1 cut(s) 1825
Eco130I CCWWGG 4 cut(s) 330, 777, 2589, 2784
Eco24I GRGCYC 2 cut(s) 1827, 2096
Eco31I GGTCTC 2 cut(s) 2043, 2054
Eco47I GGWCC 4 cut(s) 799, 1354, 2532, 2593
Eco53kI GAGCTC 1 cut(s) 1825
Eco57I CTGAAG 5 cut(s) 524, 1064, 1790, 2043, 2175
Eco81I CCTNAGG 2 cut(s) 483, 729
Eco88I CYCGRG 1 cut(s) 2040
EcoICRI GAGCTC 1 cut(s) 1825
EcoO109I RGGNCCY 2 cut(s) 132, 2093
EcoRI GAATTC 2 cut(s) 281, 1786
EcoRII CCWGG 4 cut(s) 128, 548, 889, 2230
EcoT14I CCWWGG 4 cut(s) 330, 777, 2589, 2784
EcoT38I GRGCYC 2 cut(s) 1827, 2096
ErhI CCWWGG 4 cut(s) 330, 777, 2589, 2784
FalI AAGNNNNNCTT 8 cut(s) 522, 554, 2148, 2180, 2777, 2809, 2870, 2902
FaqI GGGAC 6 cut(s) 162, 276, 587, 714, 730, 935
FauI CCCGC 2 cut(s) 496, 2505
FbaI TGATCA 3 cut(s) 1282, 2418, 2599
FblI GTMKAC 2 cut(s) 11, 2832
FokI GGATG 4 cut(s) 824, 959, 1160, 1229
FriOI GRGCYC 2 cut(s) 1827, 2096
FspBI CTAG 6 cut(s) 152, 1445, 1502, 2076, 2120, 2736
GlaI GCGC 4 cut(s) 236, 476, 999, 1799
GsaI CCCAGC 2 cut(s) 429, 2875
GsuI CTGGAG 2 cut(s) 1029, 1739
HaeII RGCGCY 2 cut(s) 478, 1801
HgaI GACGC 5 cut(s) 25, 711, 1247, 1563, 2902
HhaI GCGC 4 cut(s) 237, 477, 1000, 1800
Hin1I GRCGYC 2 cut(s) 36, 703
Hin6I GCGC 4 cut(s) 235, 475, 998, 1798
HinP1I GCGC 4 cut(s) 235, 475, 998, 1798
HincII GTYRAC 3 cut(s) 12, 1705, 2833
HindII GTYRAC 3 cut(s) 12, 1705, 2833
HindIII AAGCTT 3 cut(s) 957, 1928, 2937
HphI GGTGA 5 cut(s) 93, 109, 506, 896, 2398
Hpy166II GTNNAC 7 cut(s) 12, 887, 1373, 1705, 1908, 2444, 2833
Hpy8I GTNNAC 7 cut(s) 12, 887, 1373, 1705, 1908, 2444, 2833
Hpy99I CGWCG 5 cut(s) 13, 41, 319, 411, 1263
HpyAV CCTTC 7 cut(s) 252, 367, 556, 782, 911, 1039, 2360
HpyCH4IV ACGT 2 cut(s) 520, 1984
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 302, 928, 937, 1004, 1148, 1645, 2746, 2872
HpySE526I ACGT 2 cut(s) 520, 1984
Hsp92I GRCGYC 2 cut(s) 36, 703
HspAI GCGC 4 cut(s) 235, 475, 998, 1798
Ksp22I TGATCA 3 cut(s) 1282, 2418, 2599
LmnI GCTCC 6 cut(s) 242, 592, 1010, 1255, 1822, 2230
LweI GCATC 5 cut(s) 352, 846, 1138, 1354, 2799
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 6 cut(s) 152, 1445, 1502, 2076, 2120, 2736
MaeII ACGT 2 cut(s) 520, 1984
MaeIII GTNAC 7 cut(s) 494, 1063, 1403, 1548, 1870, 2374, 2842
MflI RGATCY 8 cut(s) 724, 766, 1104, 1243, 1722, 1999, 2056, 2670
MhlI GDGCHC 4 cut(s) 1375, 1827, 2096, 2227
MlsI TGGCCA 2 cut(s) 329, 2862
MluNI TGGCCA 2 cut(s) 329, 2862
MlyI GAGTC 8 cut(s) 208, 491, 569, 752, 1669, 1789, 2375, 2646
MmeI TCCRAC 2 cut(s) 389, 2610
Mox20I TGGCCA 2 cut(s) 329, 2862
MroXI GAANNNNTTC 1 cut(s) 2028
MscI TGGCCA 2 cut(s) 329, 2862
MseI TTAA 6 cut(s) 645, 962, 1163, 1619, 1752, 2457
MslI CAYNNNNRTG 4 cut(s) 290, 1983, 2087, 2331
Msp20I TGGCCA 2 cut(s) 329, 2862
MspA1I CMGCKG 5 cut(s) 1639, 2035, 2263, 2512, 2690
MspR9I CCNGG 8 cut(s) 130, 146, 160, 193, 550, 803, 891, 2232
Mva1269I GAATGC 1 cut(s) 1680
MvaI CCWGG 4 cut(s) 130, 550, 891, 2232
MwoI GCNNNNNNNGC 9 cut(s) 207, 302, 928, 937, 1004, 1148, 1645, 2746, 2872
NciI CCSGG 4 cut(s) 146, 160, 193, 803
NcoI CCATGG 1 cut(s) 777
NlaIV GGNNCC 8 cut(s) 134, 190, 801, 1355, 2001, 2094, 2095, 2950
NmeAIII GCCGAG 1 cut(s) 2691
NmuCI GTSAC 3 cut(s) 494, 1548, 2842
NspI RCATGY 2 cut(s) 2146, 2204
PaeI GCATGC 1 cut(s) 2204
PaeR7I CTCGAG 1 cut(s) 2040
PagI TCATGA 2 cut(s) 1671, 2553
PaqCI CACCTGC 2 cut(s) 822, 1115
PciI ACATGT 1 cut(s) 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 1 cut(s) 1680
PdmI GAANNNNTTC 1 cut(s) 2028
PfeI GAWTC 7 cut(s) 689, 844, 967, 1070, 2557, 2755, 2912
PflMI CCANNNNNTGG 3 cut(s) 896, 2590, 2729
PinAI ACCGGT 1 cut(s) 2099
PleI GAGTC 8 cut(s) 208, 491, 569, 751, 1669, 1788, 2375, 2645
PpsI GAGTC 8 cut(s) 208, 491, 569, 751, 1669, 1788, 2375, 2645
PscI ACATGT 1 cut(s) 2142
Psp124BI GAGCTC 1 cut(s) 1827
Psp6I CCWGG 4 cut(s) 128, 548, 889, 2230
PspFI CCCAGC 2 cut(s) 425, 2871
PspGI CCWGG 4 cut(s) 128, 548, 889, 2230
PspN4I GGNNCC 8 cut(s) 134, 190, 801, 1355, 2001, 2094, 2095, 2950
PspOMI GGGCCC 1 cut(s) 2092
PstI CTGCAG 1 cut(s) 2008
PstNI CAGNNNCTG 2 cut(s) 2021, 2915
PsuI RGATCY 8 cut(s) 724, 766, 1104, 1243, 1722, 1999, 2056, 2670
PvuII CAGCTG 3 cut(s) 1639, 2263, 2690
RsaI GTAC 4 cut(s) 1053, 2189, 2450, 2573
RsaNI GTAC 4 cut(s) 1052, 2188, 2449, 2572
RseI CAYNNNNRTG 4 cut(s) 290, 1983, 2087, 2331
SacI GAGCTC 1 cut(s) 1827
SalI GTCGAC 2 cut(s) 10, 2831
SaqAI TTAA 6 cut(s) 645, 962, 1163, 1619, 1752, 2457
SchI GAGTC 8 cut(s) 208, 491, 569, 752, 1669, 1789, 2375, 2646
ScrFI CCNGG 8 cut(s) 130, 146, 160, 193, 550, 803, 891, 2232
SduI GDGCHC 4 cut(s) 1375, 1827, 2096, 2227
SexAI ACCWGGT 1 cut(s) 889
SfaNI GCATC 5 cut(s) 352, 846, 1138, 1354, 2799
SfcI CTRYAG 3 cut(s) 684, 1934, 2004
Sfr274I CTCGAG 1 cut(s) 2040
SgrAI CRCCGGYG 1 cut(s) 2099
SinI GGWCC 4 cut(s) 799, 1354, 2532, 2593
SlaI CTCGAG 1 cut(s) 2040
SmiMI CAYNNNNRTG 4 cut(s) 290, 1983, 2087, 2331
SmlI CTYRAG 8 cut(s) 383, 449, 850, 1055, 1598, 2040, 2209, 2794
SmoI CTYRAG 8 cut(s) 383, 449, 850, 1055, 1598, 2040, 2209, 2794
SphI GCATGC 1 cut(s) 2204
SsiI CCGC 9 cut(s) 305, 503, 1192, 1357, 1594, 2035, 2392, 2512, 2659
SspI AATATT 1 cut(s) 642
SspMI CTAG 6 cut(s) 152, 1445, 1502, 2076, 2120, 2736
SstI GAGCTC 1 cut(s) 1827
StyD4I CCNGG 8 cut(s) 128, 144, 158, 191, 548, 801, 889, 2230
StyI CCWWGG 4 cut(s) 330, 777, 2589, 2784
TaiI ACGT 2 cut(s) 523, 1987
TaqI TCGA 8 cut(s) 11, 509, 1310, 1440, 1688, 1827, 2041, 2832
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 4 cut(s) 1051, 2187, 2448, 2571
TauI GCSGC 1 cut(s) 2394
TfiI GAWTC 7 cut(s) 689, 844, 967, 1070, 2557, 2755, 2912
Tru1I TTAA 6 cut(s) 645, 962, 1163, 1619, 1752, 2457
Tru9I TTAA 6 cut(s) 645, 962, 1163, 1619, 1752, 2457
TseFI GTSAC 3 cut(s) 494, 1548, 2842
Tsp45I GTSAC 3 cut(s) 494, 1548, 2842
TspGWI ACGGA 1 cut(s) 2475
Van91I CCANNNNNTGG 3 cut(s) 896, 2590, 2729
VneI GTGCAC 1 cut(s) 1371
VpaK11BI GGWCC 4 cut(s) 799, 1354, 2532, 2593
XapI RAATTY 3 cut(s) 281, 1786, 2355
XceI RCATGY 2 cut(s) 2146, 2204
XcmI CCANNNNNNNNNTGG 2 cut(s) 1115, 1471
XhoI CTCGAG 1 cut(s) 2040
XmiI GTMKAC 2 cut(s) 11, 2832
XmnI GAANNNNTTC 1 cut(s) 2028
XspI CTAG 6 cut(s) 152, 1445, 1502, 2076, 2120, 2736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.