Rh2CG572200

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009881
Physical Location & Seq
Forward (+)
75153647 .. 75159688
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG572200.1

Sequence Viewer

Length: 2448 bp
ATGTTAGGACGTGATTTGGAAGCCTTGCTTCAGAGAGCCAGGGATTACAAGAAGGAATATGGGGACTCGTTTGTGTCTGTGGAGCATTTGGTTCTTGGTTTCATTCAAGATCAACGATTTGGGAAGCAAATATTTAAGGATTTTCAAATCTCCAAGCAGTCTTTGAAGTCGGCTATAGAATCCATAAGGGGACGGCAATCAGTTATTGACCAAGATCCTGAGGGGAAATATGAGTCCCTTGAAAAGTATGGAAAAGATCTGACAGCCATGGCAAAAGAAGGAAAGCTTGACCCGGTAATAGGAAGAGATGATGAAATACGCAGGTGCATCCAGATTCTCTCAAGAAGAACAAAGAATAACCCAGTGCTGATTGGTGAACCAGGTGTTGGGAAAACTGCGATTTCAGAAGGGCTTGCTCAGCGAATTGTGCAAGGGGATGTCCCACAAGCTTTAATGAATCGTAAGCTAATATCTCTTGACATGGGTGCACTAATTGCTGGAGCAAAATATCGGGGAGAGTTTGAGGATAGGCTGAAGGCTGTACTCAAGGAAGTAACAGAATCAGAGGGCCAGATTATCCTTTTTATTGATGAGATCCACACAGTTGTCGGGGCAGGTGCTACAAATGGTGCAATGGATGCTGGCAATCTTTTAAAACCCATGCTTGGTCGGGGAGAGTTGCGGTGTATAGGTGCAACAACACTGGATGAATATCGCAAATATATTGAGAAAGATCCAGCATTGGAGCGTCGTTTTCAGCAAGTTTATGTTGATCAACCTACTGTTGAGGATACAATTTCGATACTCCGGGGATTGCGTGAAAGATATGAGTTGCATCACGGGGTCCGCATTTCTGATGGTGCACTTGTGGAAGCTGCAATTCTCTCAGACCGTTACATCAGTGGGAGATTTTTACCTGACAAAGCTATTGACTTAGTTGATGAAGCTGCTGCTAAATTGAAAATGGAAATTACATCAAAGCCCACAGCCCTAGATGAGATCAATCGTTCAGTATTGAAACTTGAAATGGAAAGACTGTCACTTACAAATGACACCGACAAAGCGTCCAAAGAGAGATTGAACCGCCTTGAGGCAGAGTTGTCCATCTTAAAAGAGAAACAATCTCAGCTGTCTGAGCAGTGGGAACATGAGAAGTCTGTCATGACTCGCATTCAGTCAATCAAGGAAGAGGTTGACAGGGTAAATCTGGAGATCCAGCAGGCTGAACGAGAGTATGATCTTAACCGTGCTGCTGAGCTGAAGTATGGAAGTCTGAATTCCTTACAGCGCCAACTCCTTGGTGCTGAAAAGGAGCTCGATGAGTATATCAGGTCTGGGAAATCAATGCTGAGGGAGGAAGTTACGGGAAATGACATTTCTGAAATTGTCAGCAAGTGGACTGGTATTCCTGTTTCCAAGCTTCTACAGTCAGAGAGGGAAAAGCTCTTGCATTTGGAGGAAGAGCTGCATAAACGTGTTGTAGGACAGGATCCTGCAGTGAAATCAGTAGCTGAAGCTATTCAGCGGTCTCGAGCAGGTCTCTCAGATCCACATCGCCCGATCGCTAGTTTCATGTTCATGGGCCCCACCGGTGTAGGGAAGACAGAACTAGCCAAGACCCTTGCTTCCTACATGTTCAACACTGAAGAAGCTCTTGTTAGAATTGATATGAGTGAGTACATGGAAAAGCATGCTGTCTCAAGACTGATAGGAGCTCCACCTGGCTATGTGGGGTATGAGGAGGGAGGACAGCTGACGGAGGTTGTTCGCCGGAGGCCATATTCAGTTATCCTATTTGACGAGATTGAAAAGGCGCATTCTGATGTGTTCAATGTTTTCCTTCAAATTTTAGATGATGGGAGAGTAACTGACTCACAGGGCCGCACTGTGAGTTTCACCAACACCGTGATCATTATGACCTCAAATGTTGGTTCACAGTACATACTTAATGGAGATGATGAAGTATCGCCAAAGGAGTTAGGTTATGAAACTATAAAGCAGCGGGTCATGGAGGCTGCAAGGTCCATATTTCGCCCTGAGTTCATGAATCGGGTTGATGAGTACATAGTTTTCCAGCCCTTGGACCGTGATCAGATAAACAACATTGTCAAGATACAGTTGCAACGAGTCCAAAAGAGAATTGCAGACCGCAAAATGAAGATCCAAGTGAGCGAGGCAGCTGTGCAGCTTCTTGGAAATCTCGGCTACGATCCAAACTATGGTGCTAGGCCAGTCAAGCGAGTGATTCAGCAATATGTGGAAAATGAGCTTGCCAAGGGCATCTTGAGGGGAGAGTTTAAAGAAGAAGACATCATTTTGGTCGACACAGAGGTCACGGCGTTTGGCAATGGCCAGCTACCCCAGCAAAAGCTAGTCTTCAAGACGCTTGAAACTGGTTCTGAATCTCCTGCAGCGGAGAACAAAGAAGCTTTGTCAGGGGCCGTTTAA

Protein Analysis

815

Amino Acids

91.87

Weight (kDa)

5.49

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 2 - 47 1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 104 - 205 5.2e-06 SMAX1 nucleotide binding domain
AAA PF00004 122 - 253 1.4e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 261 - 363 5.6e-34 AAA lid domain
Sigma54_activat PF00158 496 - 642 3.8e-06 Sigma-54 interaction domain
AAA_2 PF07724 519 - 687 6.5e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 524 - 645 1.5e-11 AAA domain (dynein-related subfamily)
AAA PF00004 525 - 641 1.3e-08 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 694 - 773 2.1e-26 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 312, 605
AasI GACNNNNNNGTC 1 cut(s) 2330
Acc36I ACCTGC 3 cut(s) 312, 605, 1526
AccB7I CCANNNNNTGG 3 cut(s) 386, 2080, 2219
AccI GTMKAC 1 cut(s) 2322
AciI CCGC 8 cut(s) 682, 847, 1084, 1525, 1882, 2002, 2149, 2414
AclWI GGATC 9 cut(s) 209, 589, 728, 1207, 1484, 1497, 1541, 2155, 2204
AcoI YGGCCR 1 cut(s) 2350
AcsI RAATTY 2 cut(s) 1276, 1845
AcuI CTGAAG 5 cut(s) 14, 554, 1280, 1533, 1665
AfaI GTAC 4 cut(s) 543, 1679, 1940, 2063
AfiI CCNNNNNNNGG 7 cut(s) 299, 386, 665, 1090, 1596, 2080, 2219
AflIII ACRYGT 2 cut(s) 1474, 1632
AgeI ACCGGT 1 cut(s) 1589
AhdI GACNNNNNGTC 1 cut(s) 1063
AjiI CACGTC 1 cut(s) 11
AjnI CCWGG 3 cut(s) 38, 379, 1720
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
Alw21I GWGCWC 4 cut(s) 490, 865, 1317, 1717
Alw26I GTCTC 3 cut(s) 1533, 1544, 1702
Alw44I GTGCAC 2 cut(s) 486, 861
AlwI GGATC 9 cut(s) 209, 589, 728, 1207, 1484, 1497, 1541, 2155, 2204
AlwNI CAGNNNCTG 1 cut(s) 1511
Ama87I CYCGRG 1 cut(s) 1530
AoxI GGCC 7 cut(s) 568, 1582, 1775, 1879, 2228, 2350, 2439
ApaI GGGCCC 1 cut(s) 1586
ApaLI GTGCAC 2 cut(s) 486, 861
ApoI RAATTY 2 cut(s) 1276, 1845
AsiGI ACCGGT 1 cut(s) 1589
Asp700I GAANNNNTTC 1 cut(s) 1518
AspLEI GCGC 2 cut(s) 1290, 1816
AspS9I GGNCC 8 cut(s) 568, 844, 1582, 1583, 1879, 2022, 2083, 2439
AsuC2I CCSGG 2 cut(s) 293, 809
AsuHPI GGTGA 2 cut(s) 386, 1888
AvaI CYCGRG 1 cut(s) 1530
AvaII GGWCC 3 cut(s) 844, 2022, 2083
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 3 cut(s) 490, 865, 1586
BalI TGGCCA 1 cut(s) 2352
BamHI GGATCC 1 cut(s) 1489
BanII GRGCYC 3 cut(s) 1317, 1586, 1717
BbsI GAAGAC 3 cut(s) 1607, 2313, 2368
Bbv12I GWGCWC 4 cut(s) 490, 865, 1317, 1717
BbvCI CCTCAGC 1 cut(s) 1349
BccI CCATC 3 cut(s) 851, 1112, 1850
BceAI ACGGC 3 cut(s) 209, 2352, 2426
BciT130I CCWGG 3 cut(s) 40, 381, 1722
BciVI GTATCC 1 cut(s) 784
BclI TGATCA 3 cut(s) 772, 1908, 2089
BcnI CCSGG 2 cut(s) 293, 809
BcoDI GTCTC 3 cut(s) 1533, 1544, 1702
BfaI CTAG 5 cut(s) 992, 1566, 1610, 2226, 2372
BfmI CTRYAG 4 cut(s) 174, 1424, 1494, 2409
BfoI RGCGCY 1 cut(s) 1291
BfuAI ACCTGC 3 cut(s) 312, 605, 1526
BfuI GTATCC 1 cut(s) 784
BglII AGATCT 1 cut(s) 256
BlpI GCTNAGC 2 cut(s) 417, 1254
Bme1390I CCNGG 5 cut(s) 40, 293, 381, 809, 1722
Bme18I GGWCC 3 cut(s) 844, 2022, 2083
BmeRI GACNNNNNGTC 1 cut(s) 1063
BmeT110I CYCGRG 1 cut(s) 1530
BmgBI CACGTC 1 cut(s) 11
BmgT120I GGNCC 8 cut(s) 568, 844, 1582, 1583, 1879, 2022, 2083, 2439
BmiI GGNNCC 5 cut(s) 845, 1491, 1584, 1585, 2440
BmrFI CCNGG 5 cut(s) 40, 293, 381, 809, 1722
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 4 cut(s) 336, 628, 844, 2289
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 3 cut(s) 1607, 2313, 2368
BplI GAGNNNNNCTC 2 cut(s) 1524, 1556
BpmI CTGGAG 2 cut(s) 519, 1229
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu1102I GCTNAGC 2 cut(s) 417, 1254
BpuEI CTTGAG 5 cut(s) 325, 530, 1109, 1684, 2305
BpuMI CCSGG 2 cut(s) 293, 809
BsaBI GATNNNNATC 2 cut(s) 711, 1551
BsaI GGTCTC 2 cut(s) 1533, 1544
BsaJI CCNNGG 6 cut(s) 39, 267, 808, 1297, 2079, 2274
BsaWI WCCGGW 1 cut(s) 1589
Bsc4I CCNNNNNNNGG 7 cut(s) 299, 386, 665, 1090, 1596, 2080, 2219
Bse118I RCCGGY 1 cut(s) 1589
Bse1I ACTGG 5 cut(s) 362, 708, 1405, 2231, 2398
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 639, 2353
Bse8I GATNNNNATC 2 cut(s) 711, 1551
BseBI CCWGG 3 cut(s) 40, 381, 1722
BseDI CCNNGG 6 cut(s) 39, 267, 808, 1297, 2079, 2274
BseGI GGATG 4 cut(s) 327, 442, 643, 712
BseJI GATNNNNATC 2 cut(s) 711, 1551
BseLI CCNNNNNNNGG 7 cut(s) 299, 386, 665, 1090, 1596, 2080, 2219
BseMI GCAATG 2 cut(s) 639, 2353
BseMII CTCAG 9 cut(s) 210, 431, 900, 1125, 1139, 1245, 1340, 1557, 2028
BseNI ACTGG 5 cut(s) 362, 708, 1405, 2231, 2398
BseRI GAGGAG 1 cut(s) 1754
BseSI GKGCMC 3 cut(s) 490, 865, 1586
BseYI CCCAGC 1 cut(s) 2361
BsgI GTGCAG 1 cut(s) 2204
Bsh1285I CGRYCG 1 cut(s) 1563
BshFI GGCC 7 cut(s) 570, 1584, 1777, 1881, 2230, 2352, 2441
BshTI ACCGGT 1 cut(s) 1589
BsiEI CGRYCG 1 cut(s) 1563
BsiHKAI GWGCWC 4 cut(s) 490, 865, 1317, 1717
BsiHKCI CYCGRG 1 cut(s) 1530
BsiSI CCGG 4 cut(s) 293, 808, 1590, 1771
BslFI GGGAC 4 cut(s) 77, 204, 220, 425
BslI CCNNNNNNNGG 7 cut(s) 299, 386, 665, 1090, 1596, 2080, 2219
BsmAI GTCTC 3 cut(s) 1533, 1544, 1702
BsmFI GGGAC 4 cut(s) 77, 204, 220, 425
BsmI GAATGC 2 cut(s) 1170, 1816
BsnI GGCC 7 cut(s) 570, 1584, 1777, 1881, 2230, 2352, 2441
Bso31I GGTCTC 2 cut(s) 1533, 1544
BsoBI CYCGRG 1 cut(s) 1530
Bsp120I GGGCCC 1 cut(s) 1582
Bsp1286I GDGCHC 5 cut(s) 490, 865, 1317, 1586, 1717
Bsp1720I GCTNAGC 2 cut(s) 417, 1254
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 8 cut(s) 682, 847, 1084, 1525, 1882, 2002, 2149, 2414
BspANI GGCC 7 cut(s) 570, 1584, 1777, 1881, 2230, 2352, 2441
BspCNI CTCAG 9 cut(s) 211, 430, 899, 1126, 1138, 1246, 1341, 1556, 2029
BspHI TCATGA 2 cut(s) 1161, 2043
BspLI GGNNCC 5 cut(s) 845, 1491, 1584, 1585, 2440
BspMAI CTGCAG 2 cut(s) 1498, 2413
BspMI ACCTGC 3 cut(s) 312, 605, 1526
BspPI GGATC 9 cut(s) 209, 589, 728, 1207, 1484, 1497, 1541, 2155, 2204
BspQI GCTCTTC 1 cut(s) 1455
BspTNI GGTCTC 2 cut(s) 1533, 1544
BsrDI GCAATG 2 cut(s) 639, 2353
BsrFI RCCGGY 1 cut(s) 1589
BsrI ACTGG 5 cut(s) 362, 708, 1405, 2231, 2398
BssAI RCCGGY 1 cut(s) 1589
BssECI CCNNGG 6 cut(s) 39, 267, 808, 1297, 2079, 2274
BssT1I CCWWGG 4 cut(s) 267, 1297, 2079, 2274
Bst2UI CCWGG 3 cut(s) 40, 381, 1722
Bst6I CTCTTC 3 cut(s) 298, 1182, 1455
BstAPI GCANNNNNTGC 2 cut(s) 494, 638
BstC8I GCNNGC 6 cut(s) 414, 643, 1221, 1692, 2271, 2354
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 4 cut(s) 327, 442, 643, 712
BstH2I RGCGCY 1 cut(s) 1291
BstHHI GCGC 2 cut(s) 1290, 1816
BstMAI GTCTC 3 cut(s) 1533, 1544, 1702
BstMCI CGRYCG 1 cut(s) 1563
BstMWI GCNNNNNNNGC 7 cut(s) 418, 427, 494, 638, 1135, 2236, 2362
BstNI CCWGG 3 cut(s) 40, 381, 1722
BstNSI RCATGY 2 cut(s) 1636, 1694
BstSCI CCNGG 5 cut(s) 38, 291, 379, 807, 1720
BstSFI CTRYAG 4 cut(s) 174, 1424, 1494, 2409
BstSLI GKGCMC 3 cut(s) 490, 865, 1586
BstV2I GAAGAC 3 cut(s) 1607, 2313, 2368
BstX2I RGATCY 8 cut(s) 214, 256, 594, 733, 1212, 1489, 1546, 2160
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 8 cut(s) 214, 256, 594, 733, 1212, 1489, 1546, 2160
Bsu36I CCTNAGG 1 cut(s) 219
BsuI GTATCC 1 cut(s) 784
BsuRI GGCC 7 cut(s) 570, 1584, 1777, 1881, 2230, 2352, 2441
BtgI CCRYGG 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 1538
BtrI CACGTC 1 cut(s) 11
BtsCI GGATG 4 cut(s) 327, 442, 643, 712
BtsI GCAGTG 2 cut(s) 1145, 1503
BtsIMutI CAGTG 7 cut(s) 369, 701, 907, 1145, 1503, 1641, 1884
BveI ACCTGC 3 cut(s) 312, 605, 1526
Cac8I GCNNGC 6 cut(s) 414, 643, 1221, 1692, 2271, 2354
CaiI CAGNNNCTG 1 cut(s) 1511
CciI TCATGA 2 cut(s) 1161, 2043
CfoI GCGC 2 cut(s) 1290, 1816
Cfr10I RCCGGY 1 cut(s) 1589
Cfr13I GGNCC 8 cut(s) 568, 844, 1582, 1583, 1879, 2022, 2083, 2439
CseI GACGC 3 cut(s) 737, 1053, 2392
CsiI ACCWGGT 1 cut(s) 379
Csp6I GTAC 4 cut(s) 542, 1678, 1939, 2062
CspAI ACCGGT 1 cut(s) 1589
CviQI GTAC 4 cut(s) 542, 1678, 1939, 2062
DraI TTTAAA 2 cut(s) 654, 2299
DrdI GACNNNNNNGTC 1 cut(s) 2330
DriI GACNNNNNGTC 1 cut(s) 1063
DseDI GACNNNNNNGTC 1 cut(s) 2330
EaeI YGGCCR 1 cut(s) 2350
Eam1104I CTCTTC 3 cut(s) 298, 1182, 1455
Eam1105I GACNNNNNGTC 1 cut(s) 1063
EarI CTCTTC 3 cut(s) 298, 1182, 1455
Ecl136II GAGCTC 2 cut(s) 1315, 1715
Eco130I CCWWGG 4 cut(s) 267, 1297, 2079, 2274
Eco24I GRGCYC 3 cut(s) 1317, 1586, 1717
Eco31I GGTCTC 2 cut(s) 1533, 1544
Eco47I GGWCC 3 cut(s) 844, 2022, 2083
Eco53kI GAGCTC 2 cut(s) 1315, 1715
Eco57I CTGAAG 5 cut(s) 14, 554, 1280, 1533, 1665
Eco81I CCTNAGG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 1530
EcoICRI GAGCTC 2 cut(s) 1315, 1715
EcoO109I RGGNCCY 1 cut(s) 1583
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 3 cut(s) 38, 379, 1720
EcoT14I CCWWGG 4 cut(s) 267, 1297, 2079, 2274
EcoT38I GRGCYC 3 cut(s) 1317, 1586, 1717
ErhI CCWWGG 4 cut(s) 267, 1297, 2079, 2274
FaqI GGGAC 4 cut(s) 77, 204, 220, 425
FauI CCCGC 1 cut(s) 1995
FbaI TGATCA 3 cut(s) 772, 1908, 2089
FblI GTMKAC 1 cut(s) 2322
FokI GGATG 4 cut(s) 314, 449, 650, 719
FriOI GRGCYC 3 cut(s) 1317, 1586, 1717
FspBI CTAG 5 cut(s) 992, 1566, 1610, 2226, 2372
GlaI GCGC 2 cut(s) 1289, 1815
GsaI CCCAGC 1 cut(s) 2365
GsuI CTGGAG 2 cut(s) 519, 1229
HaeII RGCGCY 1 cut(s) 1291
HaeIII GGCC 7 cut(s) 570, 1584, 1777, 1881, 2230, 2352, 2441
HapII CCGG 4 cut(s) 293, 808, 1590, 1771
HgaI GACGC 3 cut(s) 737, 1053, 2392
HhaI GCGC 2 cut(s) 1290, 1816
Hin6I GCGC 2 cut(s) 1288, 1814
HinP1I GCGC 2 cut(s) 1288, 1814
HincII GTYRAC 2 cut(s) 1195, 2323
HindII GTYRAC 2 cut(s) 1195, 2323
HindIII AAGCTT 4 cut(s) 284, 447, 1418, 2427
HpaII CCGG 4 cut(s) 293, 808, 1590, 1771
HphI GGTGA 2 cut(s) 386, 1888
Hpy166II GTNNAC 7 cut(s) 377, 488, 863, 1195, 1398, 1934, 2323
Hpy8I GTNNAC 7 cut(s) 377, 488, 863, 1195, 1398, 1934, 2323
Hpy99I CGWCG 1 cut(s) 753
HpyAV CCTTC 5 cut(s) 46, 272, 401, 529, 1850
HpyCH4IV ACGT 2 cut(s) 10, 1474
HpyF10VI GCNNNNNNNGC 7 cut(s) 418, 427, 494, 638, 1135, 2236, 2362
HpySE526I ACGT 2 cut(s) 10, 1474
HspAI GCGC 2 cut(s) 1288, 1814
Ksp22I TGATCA 3 cut(s) 772, 1908, 2089
LguI GCTCTTC 1 cut(s) 1455
LmnI GCTCC 6 cut(s) 82, 500, 745, 1312, 1712, 1720
LweI GCATC 4 cut(s) 336, 628, 844, 2289
MabI ACCWGGT 1 cut(s) 379
MaeI CTAG 5 cut(s) 992, 1566, 1610, 2226, 2372
MaeII ACGT 2 cut(s) 10, 1474
MaeIII GTNAC 6 cut(s) 553, 893, 1038, 1360, 1864, 2332
MflI RGATCY 8 cut(s) 214, 256, 594, 733, 1212, 1489, 1546, 2160
MhlI GDGCHC 5 cut(s) 490, 865, 1317, 1586, 1717
MlsI TGGCCA 1 cut(s) 2352
MluNI TGGCCA 1 cut(s) 2352
MlyI GAGTC 5 cut(s) 59, 242, 1159, 1865, 2136
Mox20I TGGCCA 1 cut(s) 2352
MroXI GAANNNNTTC 1 cut(s) 1518
MscI TGGCCA 1 cut(s) 2352
MseI TTAA 8 cut(s) 135, 452, 653, 1109, 1242, 1947, 2298, 2446
MslI CAYNNNNRTG 3 cut(s) 1473, 1577, 1821
Msp20I TGGCCA 1 cut(s) 2352
MspA1I CMGCKG 6 cut(s) 1129, 1525, 1753, 2002, 2180, 2414
MspI CCGG 4 cut(s) 293, 808, 1590, 1771
MspR9I CCNGG 5 cut(s) 40, 293, 381, 809, 1722
Mva1269I GAATGC 2 cut(s) 1170, 1816
MvaI CCWGG 3 cut(s) 40, 381, 1722
MwoI GCNNNNNNNGC 7 cut(s) 418, 427, 494, 638, 1135, 2236, 2362
NciI CCSGG 2 cut(s) 293, 809
NcoI CCATGG 1 cut(s) 267
NlaIV GGNNCC 5 cut(s) 845, 1491, 1584, 1585, 2440
NmeAIII GCCGAG 1 cut(s) 2181
NmuCI GTSAC 2 cut(s) 1038, 2332
NspI RCATGY 2 cut(s) 1636, 1694
PaeI GCATGC 1 cut(s) 1694
PaeR7I CTCGAG 1 cut(s) 1530
PagI TCATGA 2 cut(s) 1161, 2043
PaqCI CACCTGC 2 cut(s) 312, 605
PciI ACATGT 1 cut(s) 1632
PciSI GCTCTTC 1 cut(s) 1455
PctI GAATGC 2 cut(s) 1170, 1816
PdmI GAANNNNTTC 1 cut(s) 1518
PfeI GAWTC 7 cut(s) 179, 334, 457, 560, 2047, 2245, 2402
PflMI CCANNNNNTGG 3 cut(s) 386, 2080, 2219
PinAI ACCGGT 1 cut(s) 1589
Ple19I CGATCG 1 cut(s) 1563
PleI GAGTC 5 cut(s) 59, 241, 1159, 1865, 2135
PpsI GAGTC 5 cut(s) 59, 241, 1159, 1865, 2135
PscI ACATGT 1 cut(s) 1632
Psp124BI GAGCTC 2 cut(s) 1317, 1717
Psp6I CCWGG 3 cut(s) 38, 379, 1720
PspFI CCCAGC 1 cut(s) 2361
PspGI CCWGG 3 cut(s) 38, 379, 1720
PspN4I GGNNCC 5 cut(s) 845, 1491, 1584, 1585, 2440
PspOMI GGGCCC 1 cut(s) 1582
PspPI GGNCC 8 cut(s) 568, 844, 1582, 1583, 1879, 2022, 2083, 2439
PstI CTGCAG 2 cut(s) 1498, 2413
PstNI CAGNNNCTG 1 cut(s) 1511
PsuI RGATCY 8 cut(s) 214, 256, 594, 733, 1212, 1489, 1546, 2160
PvuI CGATCG 1 cut(s) 1563
PvuII CAGCTG 3 cut(s) 1129, 1753, 2180
RsaI GTAC 4 cut(s) 543, 1679, 1940, 2063
RsaNI GTAC 4 cut(s) 542, 1678, 1939, 2062
RseI CAYNNNNRTG 3 cut(s) 1473, 1577, 1821
SacI GAGCTC 2 cut(s) 1317, 1717
SalI GTCGAC 1 cut(s) 2321
SapI GCTCTTC 1 cut(s) 1455
SaqAI TTAA 8 cut(s) 135, 452, 653, 1109, 1242, 1947, 2298, 2446
Sau96I GGNCC 8 cut(s) 568, 844, 1582, 1583, 1879, 2022, 2083, 2439
SchI GAGTC 5 cut(s) 59, 242, 1159, 1865, 2136
ScrFI CCNGG 5 cut(s) 40, 293, 381, 809, 1722
SduI GDGCHC 5 cut(s) 490, 865, 1317, 1586, 1717
SexAI ACCWGGT 1 cut(s) 379
SfaNI GCATC 4 cut(s) 336, 628, 844, 2289
SfcI CTRYAG 4 cut(s) 174, 1424, 1494, 2409
Sfr274I CTCGAG 1 cut(s) 1530
SgrAI CRCCGGYG 1 cut(s) 1589
SinI GGWCC 3 cut(s) 844, 2022, 2083
SlaI CTCGAG 1 cut(s) 1530
SmiMI CAYNNNNRTG 3 cut(s) 1473, 1577, 1821
SmlI CTYRAG 6 cut(s) 340, 545, 1088, 1530, 1699, 2284
SmoI CTYRAG 6 cut(s) 340, 545, 1088, 1530, 1699, 2284
SphI GCATGC 1 cut(s) 1694
SsiI CCGC 8 cut(s) 682, 847, 1084, 1525, 1882, 2002, 2149, 2414
SspI AATATT 1 cut(s) 132
SspMI CTAG 5 cut(s) 992, 1566, 1610, 2226, 2372
SstI GAGCTC 2 cut(s) 1317, 1717
StyD4I CCNGG 5 cut(s) 38, 291, 379, 807, 1720
StyI CCWWGG 4 cut(s) 267, 1297, 2079, 2274
TaiI ACGT 2 cut(s) 13, 1477
TaqI TCGA 4 cut(s) 800, 1317, 1531, 2322
TatI WGTACW 4 cut(s) 541, 1677, 1938, 2061
TauI GCSGC 1 cut(s) 1884
TfiI GAWTC 7 cut(s) 179, 334, 457, 560, 2047, 2245, 2402
Tru1I TTAA 8 cut(s) 135, 452, 653, 1109, 1242, 1947, 2298, 2446
Tru9I TTAA 8 cut(s) 135, 452, 653, 1109, 1242, 1947, 2298, 2446
TscAI CASTG 7 cut(s) 369, 708, 907, 1145, 1503, 1648, 1891
TseFI GTSAC 2 cut(s) 1038, 2332
Tsp45I GTSAC 2 cut(s) 1038, 2332
TspGWI ACGGA 1 cut(s) 1772
TspRI CASTG 7 cut(s) 369, 708, 907, 1145, 1503, 1648, 1891
Van91I CCANNNNNTGG 3 cut(s) 386, 2080, 2219
VneI GTGCAC 2 cut(s) 486, 861
VpaK11BI GGWCC 3 cut(s) 844, 2022, 2083
XapI RAATTY 2 cut(s) 1276, 1845
XceI RCATGY 2 cut(s) 1636, 1694
XhoI CTCGAG 1 cut(s) 1530
XmiI GTMKAC 1 cut(s) 2322
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 5 cut(s) 992, 1566, 1610, 2226, 2372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.