FvH4_6g47410

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009881
Physical Location & Seq
Forward (+)
35930807 .. 35936918
6112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47410.t1

Sequence Viewer

Length: 2946 bp
ATGGCCTCCACGACCACCTCCTTCGCCGCTCCCGGAGTCGTCGCTCTCCGTCTTCCCCAGTCCAAATGTTGCACCAAAGCTTCTCTCTTTCCTCACCCAAACCCCTCCGTCTCCCTCCGCGCCCGGCCCCACTTCCTCCGCGGACTCACTTCCTGCCGATTCGACCCGAACTCCCCTTTTCAAACCGGGTCGGGTCGGATGACCCGGAGCCCGAACCAGTTTGTTGTGCGCGCCATGGCTTCTTCAGCTGGAAGGATTACGCAGCAGGATTTTACTGAGATGGCGTGGCAGGCGGTGGTTTCGTCGCCGGAGGTGGCCAAGGAGAACAAGCATCAGATAGTGGAGACTGAGCATTTGATGAAGGCGTTGTTGGAGCAGAAGAATGGACTTGCTAGGAGGATTTTCGCTAAGGCTGGGGTGGATAATACTCAACTTCTTGAGGCTACTGATAAGTATATTCAGCGCCAACCAAAGATTCTTGGTGAAACTGCGGGATCAATGTTAGGACGGGATTTGGAAGCCTTGCTTGAGCGAGCCAGGGATTACAAGAAAGAGTATGGAGACTCATTTGTGTCTGTGGAGCATTTGGTTCTTGGTTTCATTCAAGATCAACGATTTGGGAAGCAATTATTTAAGGAATTTCAAATCTCCAAGCAATCTTTGAAGTCTGCTATAGAATCCATAAGGGGACGCCAATCAGTTATTGACCAAGATCCTGAGGGGAAATATGAGTCCCTTGAAAAGTACGGAAAAGATCTGACAGCCATGGCAAGACAAGGAAAGCTTGACCCGGTTATAGGAAGAGATGATGAAATCCGCAGGTGCATCCAGATTCTGTCAAGAAGAACAAAGAACAACCCGGTGCTAATTGGTGAACCAGGTGTTGGGAAAACTGCAATTTCAGAAGGGCTTGCTCAGCGTATTGTGCAAGAGGATGTCCCACAAGCTTTGATGAATCGTAAGCTAATCTCTCTTGACATGGGTTCACTAATTGCTGGGGCAAAATACCGTGGAGAGTTTGAGGATAGGCTGAAGGCTGTACTCAAGGAAGTAACAGAATCTGAAGGCCAGATTATCCTTTTTATTGATGAAATTCACACAGTTGTCGGGGCAGGAGCTACAAGTGGTGCAATGGATGCTGGAAATCTTTTAAAACCCATGCTTGGTCGGGGAGAGTTGCGGTGTATAGGTGCAACAACACTGGATGAGTATCGCAAATATATTGAGAAAGATCCAGCGTTGGAGCGTCGTTTTCAGCAAGTTTATGTTGATCAACCTACAGTTGAGGATACAATCTCAATACTCCGAGGACTGCGTGAAAGATATGAGTTGCATCACGGGGTCCGCATTTCTGATGGTGCACTTGTGGAAGCTGCAATTCTCTCAGACCGTTACATCAGTGGGAGATTTTTACCCGACAAAGCTATCGACTTAGTTGATGAAGCTGCTGCCAAATTGAAAATGGAAATTACATCAAAGCCCACAGCGCTAGATGAGATTAATCGTTCAGTGTTGAAACTTGAAATGGAAAGACTGTCACTTACAAATGACACCGACAAAGCATCCAGAGAGAGATTGAACCGCCTTGAGGCGGAGTTGTCCGTCTTAAAAGAGAAACAATCTCAGCTGTCTGAGCAGTGGGAACATGAGAAGTCTGTTATGACTCGAATTCAGTCAATCAAGGAAGAGGTTGACAGGGTAAATCTGGAGATCCAGCAGGCTGAACGAGAGTATGATCTTAACCGTGCTGCTGAGCTGAAGTATGGGAGTTTGAATTCCTTACAGCGCCAACTCCTTAGTGCTGAAAAAGAGCTCGATGAGTATATCAGGTCTGGGAAATCAATGCTGAGAGAGGAAGTTACGGGAAATGACATTTCTGAAATTGTCAGCAAGTGGACTGGTATTCCTGTTTCCAAGCTTCTACAGTCAGAGAGGGAAAAGCTCTTACATTTGGAGGAAGAGCTGCATAAACGTGTTGTAGGACAGGATCCTGCTGTGAAATCAGTAGCTGAAGCTATTCAGCGGTCTCGAGCAGGTCTCTCAGATCCTCATCGCCCGATTGCTAGTTTCATGTTCATGGGCCCCACCGGTGTAGGGAAGACAGAACTAGCCAAGACCCTTGCCTCCTACATGTTCAACACTGAAGAAGCTCTTGTTAGAATTGATATGAGTGAGTACATGGAAAAGCATGCTGTGTCAAGACTGATAGGTGCTCCACCTGGCTATGTGGGTTATGAGGAGGGAGGACAGCTGACAGAGGTTGTTCGCCGGAGGCCATATTCAGTTATCCTGTTTGACGAGATTGAAAAGGCGCATTCTGATGTGTTCAATGTTTTCCTTCAAATTTTAGATGATGGAAGAGTAACCGACTCACAGGGCCGCACTGTGAGTTTCACCAACACTGTGATCATTATGACCTCAAATGTTGGTTCACAGTATATACTAAACGGAGATGATGACGTCTCGCCGAAGGAATTGGGTTATGAAACTATAAAGCAGCGGGTGATGGAGGCTGCAAGGTCCGTATTTCGCCCCGAGTTCATGAATCGGGTTGATGAGTACATAGTTTTCCAGCCCTTGGACCGTGATCAGATAAACAATATTGTCAGGATACAGTTGGAACGAGTCCAGAGGAGGATTGCAGACCGCAAGATGAAAATCCAAGTGAGCGATGCTGCTGTGCAGCTTCTTGGAAGTCTTGGATACGATCCAAACTACGGTGCTAGGCCAGTAAAGCGAGTGATTCAGCAATATGTAGAAAATGAGCTTGCCAAGGGCATCTTGAGAGGAGAGTTCCAAGAAGAAGGCACCATTTTGGTCGACACAGAGGTCACAGCGTATGGCAACGGCCAGCTCCCCCAGCAAAAGCTAGTCTTCAAGACGCTTGAGACTGGTTCAGAATCTCCTGCAGCTGAGAACAAGGGAGCTTTGTCAGGCGCTGTCTGA

Protein Analysis

982

Amino Acids

110.04

Weight (kDa)

6.13

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 91 - 213 1e-34 Clp repeat (R) N-terminal domain
AAA PF00004 288 - 419 1.2e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 427 - 529 1e-33 AAA lid domain
Sigma54_activat PF00158 662 - 808 5e-06 Sigma-54 interaction domain
AAA_2 PF07724 685 - 853 7.5e-70 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 690 - 811 2.3e-11 AAA domain (dynein-related subfamily)
AAA PF00004 691 - 807 1.7e-08 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 860 - 940 2.9e-26 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 810
AasI GACNNNNNNGTC 1 cut(s) 2828
AatII GACGTC 1 cut(s) 2463
Acc36I ACCTGC 2 cut(s) 810, 2024
AccB1I GGYRCC 1 cut(s) 2807
AccB7I CCANNNNNTGG 2 cut(s) 884, 2578
AccBSI CCGCTC 1 cut(s) 29
AccI GTMKAC 1 cut(s) 2820
AccII CGCG 3 cut(s) 120, 141, 231
AclWI GGATC 8 cut(s) 502, 707, 1226, 1705, 1982, 1995, 2039, 2702
AcoI YGGCCR 2 cut(s) 315, 2848
AcsI RAATTY 5 cut(s) 638, 1092, 1668, 1774, 2343
AcuI CTGAAG 6 cut(s) 228, 1052, 1083, 1778, 2031, 2163
AcyI GRCGYC 2 cut(s) 691, 2460
AfaI GTAC 4 cut(s) 746, 1041, 2177, 2561
AfeI AGCGCT 1 cut(s) 1488
AfiI CCNNNNNNNGG 8 cut(s) 797, 884, 1163, 1588, 1591, 2094, 2578, 2717
AflIII ACRYGT 2 cut(s) 1972, 2130
AgeI ACCGGT 1 cut(s) 2087
AjnI CCWGG 3 cut(s) 536, 877, 2218
AjuI GAANNNNNNNTTGG 6 cut(s) 353, 385, 459, 491, 867, 899
Alw21I GWGCWC 3 cut(s) 1363, 1815, 2215
Alw26I GTCTC 7 cut(s) 115, 338, 555, 2031, 2042, 2467, 2882
Alw44I GTGCAC 1 cut(s) 1359
AlwI GGATC 8 cut(s) 502, 707, 1226, 1705, 1982, 1995, 2039, 2702
AlwNI CAGNNNCTG 4 cut(s) 835, 1061, 2009, 2939
Ama87I CYCGRG 2 cut(s) 2028, 2534
Aor51HI AGCGCT 1 cut(s) 1488
AoxI GGCC 9 cut(s) 3, 125, 315, 1066, 2080, 2273, 2377, 2726, 2848
ApaI GGGCCC 1 cut(s) 2084
ApaLI GTGCAC 1 cut(s) 1359
ApoI RAATTY 5 cut(s) 638, 1092, 1668, 1774, 2343
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AseI ATTAAT 1 cut(s) 1500
AsiGI ACCGGT 1 cut(s) 2087
Asp700I GAANNNNTTC 1 cut(s) 2016
AspLEI GCGC 8 cut(s) 122, 231, 233, 465, 1489, 1788, 2314, 2939
AspS9I GGNCC 7 cut(s) 126, 1342, 2080, 2081, 2377, 2520, 2581
AsuC2I CCSGG 6 cut(s) 33, 124, 187, 205, 791, 860
AsuHPI GGTGA 5 cut(s) 86, 494, 884, 2386, 2515
AvaI CYCGRG 2 cut(s) 2028, 2534
AvaII GGWCC 3 cut(s) 1342, 2520, 2581
AxyI CCTNAGG 1 cut(s) 717
BaeGI GKGCMC 2 cut(s) 1363, 2084
BalI TGGCCA 1 cut(s) 317
BamHI GGATCC 1 cut(s) 1987
BanI GGYRCC 1 cut(s) 2807
BanII GRGCYC 3 cut(s) 212, 1815, 2084
BbsI GAAGAC 3 cut(s) 44, 2105, 2866
Bbv12I GWGCWC 3 cut(s) 1363, 1815, 2215
BccI CCATC 4 cut(s) 274, 1349, 2348, 2500
BceAI ACGGC 1 cut(s) 2863
BciT130I CCWGG 3 cut(s) 538, 879, 2220
BciVI GTATCC 3 cut(s) 1282, 2604, 2696
BclI TGATCA 3 cut(s) 1270, 2406, 2587
BcnI CCSGG 6 cut(s) 33, 124, 187, 205, 791, 860
BcoDI GTCTC 7 cut(s) 115, 338, 555, 2031, 2042, 2467, 2882
BfaI CTAG 6 cut(s) 393, 1490, 2064, 2108, 2724, 2870
BfmI CTRYAG 4 cut(s) 672, 1278, 1922, 2907
BfoI RGCGCY 4 cut(s) 466, 1490, 1789, 2940
BfuAI ACCTGC 2 cut(s) 810, 2024
BfuI GTATCC 3 cut(s) 1282, 2604, 2696
BglII AGATCT 1 cut(s) 754
BlpI GCTNAGC 2 cut(s) 915, 1752
Bme1390I CCNGG 9 cut(s) 33, 124, 187, 205, 538, 791, 860, 879, 2220
Bme18I GGWCC 3 cut(s) 1342, 2520, 2581
BmeT110I CYCGRG 2 cut(s) 2028, 2534
BmgT120I GGNCC 7 cut(s) 126, 1342, 2080, 2081, 2377, 2520, 2581
BmiI GGNNCC 7 cut(s) 128, 209, 1343, 1989, 2082, 2083, 2809
BmrFI CCNGG 9 cut(s) 33, 124, 187, 205, 538, 791, 860, 879, 2220
BmrI ACTGGG 1 cut(s) 52
BmsI GCATC 7 cut(s) 340, 834, 1126, 1342, 1571, 2662, 2787
BmuI ACTGGG 1 cut(s) 52
BpiI GAAGAC 3 cut(s) 44, 2105, 2866
BplI GAGNNNNNCTC 2 cut(s) 2022, 2054
BpmI CTGGAG 1 cut(s) 1727
Bpu10I CCTNAGC 1 cut(s) 408
Bpu1102I GCTNAGC 2 cut(s) 915, 1752
BpuEI CTTGAG 6 cut(s) 458, 548, 1028, 1607, 2803, 2906
BpuMI CCSGG 6 cut(s) 33, 124, 187, 205, 791, 860
BsaBI GATNNNNATC 3 cut(s) 1209, 2049, 2657
BsaHI GRCGYC 2 cut(s) 691, 2460
BsaI GGTCTC 2 cut(s) 2031, 2042
BsaJI CCNNGG 9 cut(s) 139, 234, 318, 537, 765, 1009, 1306, 2577, 2772
BsaWI WCCGGW 1 cut(s) 2087
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 8 cut(s) 797, 884, 1163, 1588, 1591, 2094, 2578, 2717
Bse118I RCCGGY 1 cut(s) 2087
Bse1I ACTGG 6 cut(s) 58, 217, 1206, 1903, 2729, 2896
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 1137
Bse8I GATNNNNATC 3 cut(s) 1209, 2049, 2657
BseBI CCWGG 3 cut(s) 538, 879, 2220
BseDI CCNNGG 9 cut(s) 139, 234, 318, 537, 765, 1009, 1306, 2577, 2772
BseGI GGATG 6 cut(s) 204, 825, 940, 1141, 1210, 1562
BseJI GATNNNNATC 3 cut(s) 1209, 2049, 2657
BseLI CCNNNNNNNGG 8 cut(s) 797, 884, 1163, 1588, 1591, 2094, 2578, 2717
BseMI GCAATG 1 cut(s) 1137
BseNI ACTGG 6 cut(s) 58, 217, 1206, 1903, 2729, 2896
BsePI GCGCGC 1 cut(s) 229
BseRI GAGGAG 3 cut(s) 2252, 2647, 2802
BseSI GKGCMC 2 cut(s) 1363, 2084
BseYI CCCAGC 3 cut(s) 413, 995, 2859
BsgI GTGCAG 1 cut(s) 2702
Bsh1236I CGCG 3 cut(s) 120, 141, 231
BshFI GGCC 9 cut(s) 5, 127, 317, 1068, 2082, 2275, 2379, 2728, 2850
BshNI GGYRCC 1 cut(s) 2807
BshTI ACCGGT 1 cut(s) 2087
BsiHKAI GWGCWC 3 cut(s) 1363, 1815, 2215
BsiHKCI CYCGRG 2 cut(s) 2028, 2534
BsiSI CCGG 9 cut(s) 33, 124, 186, 205, 308, 791, 860, 2088, 2269
BslFI GGGAC 3 cut(s) 702, 718, 923
BslI CCNNNNNNNGG 8 cut(s) 797, 884, 1163, 1588, 1591, 2094, 2578, 2717
BsmAI GTCTC 7 cut(s) 115, 338, 555, 2031, 2042, 2467, 2882
BsmBI CGTCTC 2 cut(s) 115, 2467
BsmFI GGGAC 3 cut(s) 702, 718, 923
BsmI GAATGC 1 cut(s) 2314
BsnI GGCC 9 cut(s) 5, 127, 317, 1068, 2082, 2275, 2379, 2728, 2850
Bso31I GGTCTC 2 cut(s) 2031, 2042
BsoBI CYCGRG 2 cut(s) 2028, 2534
Bsp120I GGGCCC 1 cut(s) 2080
Bsp1286I GDGCHC 5 cut(s) 212, 1363, 1815, 2084, 2215
Bsp1720I GCTNAGC 2 cut(s) 915, 1752
Bsp19I CCATGG 2 cut(s) 234, 765
BspANI GGCC 9 cut(s) 5, 127, 317, 1068, 2082, 2275, 2379, 2728, 2850
BspFNI CGCG 3 cut(s) 120, 141, 231
BspHI TCATGA 1 cut(s) 2541
BspLI GGNNCC 7 cut(s) 128, 209, 1343, 1989, 2082, 2083, 2809
BspMAI CTGCAG 1 cut(s) 2911
BspMI ACCTGC 2 cut(s) 810, 2024
BspPI GGATC 8 cut(s) 502, 707, 1226, 1705, 1982, 1995, 2039, 2702
BspQI GCTCTTC 1 cut(s) 1953
BspT107I GGYRCC 1 cut(s) 2807
BspTNI GGTCTC 2 cut(s) 2031, 2042
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 1137
BsrFI RCCGGY 1 cut(s) 2087
BsrI ACTGG 6 cut(s) 58, 217, 1206, 1903, 2729, 2896
BssAI RCCGGY 1 cut(s) 2087
BssECI CCNNGG 9 cut(s) 139, 234, 318, 537, 765, 1009, 1306, 2577, 2772
BssHII GCGCGC 1 cut(s) 229
BssNI GRCGYC 2 cut(s) 691, 2460
BssT1I CCWWGG 5 cut(s) 234, 318, 765, 2577, 2772
Bst2UI CCWGG 3 cut(s) 538, 879, 2220
Bst6I CTCTTC 4 cut(s) 796, 1680, 1953, 2353
BstACI GRCGYC 2 cut(s) 691, 2460
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstC8I GCNNGC 8 cut(s) 231, 291, 534, 912, 1719, 2190, 2769, 2852
BstDSI CCRYGG 4 cut(s) 139, 234, 765, 1009
BstF5I GGATG 6 cut(s) 204, 825, 940, 1141, 1210, 1562
BstFNI CGCG 3 cut(s) 120, 141, 231
BstH2I RGCGCY 4 cut(s) 466, 1490, 1789, 2940
BstHHI GCGC 8 cut(s) 122, 231, 233, 465, 1489, 1788, 2314, 2939
BstMAI GTCTC 7 cut(s) 115, 338, 555, 2031, 2042, 2467, 2882
BstMWI GCNNNNNNNGC 9 cut(s) 245, 290, 916, 925, 1136, 1486, 1633, 2734, 2860
BstNI CCWGG 3 cut(s) 538, 879, 2220
BstNSI RCATGY 2 cut(s) 2134, 2192
BstSCI CCNGG 9 cut(s) 31, 122, 185, 203, 536, 789, 858, 877, 2218
BstSFI CTRYAG 4 cut(s) 672, 1278, 1922, 2907
BstSLI GKGCMC 2 cut(s) 1363, 2084
BstUI CGCG 3 cut(s) 120, 141, 231
BstV2I GAAGAC 3 cut(s) 44, 2105, 2866
BstX2I RGATCY 6 cut(s) 712, 754, 1231, 1710, 1987, 2044
BstYI RGATCY 6 cut(s) 712, 754, 1231, 1710, 1987, 2044
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 3 cut(s) 1282, 2604, 2696
BsuRI GGCC 9 cut(s) 5, 127, 317, 1068, 2082, 2275, 2379, 2728, 2850
BtgI CCRYGG 4 cut(s) 139, 234, 765, 1009
BtgZI GCGATG 2 cut(s) 2036, 2685
BtsCI GGATG 6 cut(s) 204, 825, 940, 1141, 1210, 1562
BtsI GCAGTG 1 cut(s) 1643
BtsIMutI CAGTG 7 cut(s) 1199, 1405, 1515, 1643, 2139, 2382, 2400
BveI ACCTGC 2 cut(s) 810, 2024
Cac8I GCNNGC 8 cut(s) 231, 291, 534, 912, 1719, 2190, 2769, 2852
CaiI CAGNNNCTG 4 cut(s) 835, 1061, 2009, 2939
CciI TCATGA 1 cut(s) 2541
CfoI GCGC 8 cut(s) 122, 231, 233, 465, 1489, 1788, 2314, 2939
Cfr10I RCCGGY 1 cut(s) 2087
Cfr13I GGNCC 7 cut(s) 126, 1342, 2080, 2081, 2377, 2520, 2581
Cfr42I CCGCGG 1 cut(s) 142
CseI GACGC 3 cut(s) 699, 1235, 2890
CsiI ACCWGGT 1 cut(s) 877
Csp6I GTAC 4 cut(s) 745, 1040, 2176, 2560
CspAI ACCGGT 1 cut(s) 2087
CspCI CAANNNNNGTGG 2 cut(s) 930, 965
CviQI GTAC 4 cut(s) 745, 1040, 2176, 2560
DraI TTTAAA 1 cut(s) 1152
DrdI GACNNNNNNGTC 1 cut(s) 2828
DseDI GACNNNNNNGTC 1 cut(s) 2828
EaeI YGGCCR 2 cut(s) 315, 2848
Eam1104I CTCTTC 4 cut(s) 796, 1680, 1953, 2353
EarI CTCTTC 4 cut(s) 796, 1680, 1953, 2353
EciI GGCGGA 1 cut(s) 1607
Ecl136II GAGCTC 1 cut(s) 1813
Eco130I CCWWGG 5 cut(s) 234, 318, 765, 2577, 2772
Eco24I GRGCYC 3 cut(s) 212, 1815, 2084
Eco31I GGTCTC 2 cut(s) 2031, 2042
Eco47I GGWCC 3 cut(s) 1342, 2520, 2581
Eco47III AGCGCT 1 cut(s) 1488
Eco53kI GAGCTC 1 cut(s) 1813
Eco57I CTGAAG 6 cut(s) 228, 1052, 1083, 1778, 2031, 2163
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 2 cut(s) 2028, 2534
EcoICRI GAGCTC 1 cut(s) 1813
EcoO109I RGGNCCY 1 cut(s) 2081
EcoRI GAATTC 2 cut(s) 1668, 1774
EcoRII CCWGG 3 cut(s) 536, 877, 2218
EcoT14I CCWWGG 5 cut(s) 234, 318, 765, 2577, 2772
EcoT38I GRGCYC 3 cut(s) 212, 1815, 2084
ErhI CCWWGG 5 cut(s) 234, 318, 765, 2577, 2772
Esp3I CGTCTC 2 cut(s) 115, 2467
FaqI GGGAC 3 cut(s) 702, 718, 923
FauI CCCGC 2 cut(s) 484, 2493
FbaI TGATCA 3 cut(s) 1270, 2406, 2587
FblI GTMKAC 1 cut(s) 2820
FokI GGATG 6 cut(s) 211, 812, 947, 1148, 1217, 1549
FriOI GRGCYC 3 cut(s) 212, 1815, 2084
FspBI CTAG 6 cut(s) 393, 1490, 2064, 2108, 2724, 2870
GlaI GCGC 8 cut(s) 121, 230, 232, 464, 1488, 1787, 2313, 2938
GsaI CCCAGC 3 cut(s) 417, 999, 2863
GsuI CTGGAG 1 cut(s) 1727
HaeII RGCGCY 4 cut(s) 466, 1490, 1789, 2940
HaeIII GGCC 9 cut(s) 5, 127, 317, 1068, 2082, 2275, 2379, 2728, 2850
HapII CCGG 9 cut(s) 33, 124, 186, 205, 308, 791, 860, 2088, 2269
HgaI GACGC 3 cut(s) 699, 1235, 2890
HhaI GCGC 8 cut(s) 122, 231, 233, 465, 1489, 1788, 2314, 2939
Hin1I GRCGYC 2 cut(s) 691, 2460
Hin6I GCGC 8 cut(s) 120, 229, 231, 463, 1487, 1786, 2312, 2937
HinP1I GCGC 8 cut(s) 120, 229, 231, 463, 1487, 1786, 2312, 2937
HincII GTYRAC 2 cut(s) 1693, 2821
HindII GTYRAC 2 cut(s) 1693, 2821
HindIII AAGCTT 4 cut(s) 78, 782, 945, 1916
HpaII CCGG 9 cut(s) 33, 124, 186, 205, 308, 791, 860, 2088, 2269
HphI GGTGA 5 cut(s) 86, 494, 884, 2386, 2515
Hpy166II GTNNAC 7 cut(s) 875, 986, 1361, 1693, 1896, 2432, 2821
Hpy8I GTNNAC 7 cut(s) 875, 986, 1361, 1693, 1896, 2432, 2821
Hpy99I CGWCG 3 cut(s) 44, 307, 1251
HpyAV CCTTC 9 cut(s) 31, 246, 355, 899, 1027, 1058, 2348, 2464, 2798
HpyCH4IV ACGT 2 cut(s) 1972, 2460
HpyF10VI GCNNNNNNNGC 9 cut(s) 245, 290, 916, 925, 1136, 1486, 1633, 2734, 2860
HpySE526I ACGT 2 cut(s) 1972, 2460
Hsp92I GRCGYC 2 cut(s) 691, 2460
HspAI GCGC 8 cut(s) 120, 229, 231, 463, 1487, 1786, 2312, 2937
Ksp22I TGATCA 3 cut(s) 1270, 2406, 2587
KspI CCGCGG 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 1953
LmnI GCTCC 9 cut(s) 34, 207, 373, 580, 1115, 1243, 2218, 2859, 2924
LweI GCATC 7 cut(s) 340, 834, 1126, 1342, 1571, 2662, 2787
MabI ACCWGGT 1 cut(s) 877
MaeI CTAG 6 cut(s) 393, 1490, 2064, 2108, 2724, 2870
MaeII ACGT 2 cut(s) 1972, 2460
MaeIII GTNAC 6 cut(s) 1051, 1391, 1536, 1858, 2362, 2830
MbiI CCGCTC 1 cut(s) 29
MflI RGATCY 6 cut(s) 712, 754, 1231, 1710, 1987, 2044
MhlI GDGCHC 5 cut(s) 212, 1363, 1815, 2084, 2215
MlsI TGGCCA 1 cut(s) 317
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 7 cut(s) 45, 138, 557, 740, 1657, 2363, 2634
MmeI TCCRAC 4 cut(s) 176, 351, 1221, 2598
Mox20I TGGCCA 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 2016
MscI TGGCCA 1 cut(s) 317
MseI TTAA 5 cut(s) 633, 1151, 1500, 1607, 1740
MslI CAYNNNNRTG 3 cut(s) 1971, 2075, 2319
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 7 cut(s) 141, 248, 1627, 2023, 2251, 2500, 2912
MspI CCGG 9 cut(s) 33, 124, 186, 205, 308, 791, 860, 2088, 2269
MspR9I CCNGG 9 cut(s) 33, 124, 187, 205, 538, 791, 860, 879, 2220
Mva1269I GAATGC 1 cut(s) 2314
MvaI CCWGG 3 cut(s) 538, 879, 2220
MvnI CGCG 3 cut(s) 120, 141, 231
MwoI GCNNNNNNNGC 9 cut(s) 245, 290, 916, 925, 1136, 1486, 1633, 2734, 2860
NciI CCSGG 6 cut(s) 33, 124, 187, 205, 791, 860
NcoI CCATGG 2 cut(s) 234, 765
NlaIV GGNNCC 7 cut(s) 128, 209, 1343, 1989, 2082, 2083, 2809
NmuCI GTSAC 2 cut(s) 1536, 2830
NspI RCATGY 2 cut(s) 2134, 2192
PaeI GCATGC 1 cut(s) 2192
PaeR7I CTCGAG 1 cut(s) 2028
PagI TCATGA 1 cut(s) 2541
PaqCI CACCTGC 1 cut(s) 810
PauI GCGCGC 1 cut(s) 229
PciI ACATGT 1 cut(s) 2130
PciSI GCTCTTC 1 cut(s) 1953
PctI GAATGC 1 cut(s) 2314
PdmI GAANNNNTTC 1 cut(s) 2016
PfeI GAWTC 9 cut(s) 159, 475, 677, 832, 955, 1058, 2545, 2743, 2900
PflMI CCANNNNNTGG 2 cut(s) 884, 2578
PfoI TCCNGGA 1 cut(s) 31
PinAI ACCGGT 1 cut(s) 2087
PleI GAGTC 7 cut(s) 44, 138, 557, 739, 1657, 2363, 2633
PpsI GAGTC 7 cut(s) 44, 138, 557, 739, 1657, 2363, 2633
PscI ACATGT 1 cut(s) 2130
PshBI ATTAAT 1 cut(s) 1500
Psp124BI GAGCTC 1 cut(s) 1815
Psp6I CCWGG 3 cut(s) 536, 877, 2218
PspFI CCCAGC 3 cut(s) 413, 995, 2859
PspGI CCWGG 3 cut(s) 536, 877, 2218
PspN4I GGNNCC 7 cut(s) 128, 209, 1343, 1989, 2082, 2083, 2809
PspOMI GGGCCC 1 cut(s) 2080
PspPI GGNCC 7 cut(s) 126, 1342, 2080, 2081, 2377, 2520, 2581
PstI CTGCAG 1 cut(s) 2911
PstNI CAGNNNCTG 4 cut(s) 835, 1061, 2009, 2939
PsuI RGATCY 6 cut(s) 712, 754, 1231, 1710, 1987, 2044
PteI GCGCGC 1 cut(s) 229
PvuII CAGCTG 4 cut(s) 248, 1627, 2251, 2912
RsaI GTAC 4 cut(s) 746, 1041, 2177, 2561
RsaNI GTAC 4 cut(s) 745, 1040, 2176, 2560
RseI CAYNNNNRTG 3 cut(s) 1971, 2075, 2319
SacI GAGCTC 1 cut(s) 1815
SacII CCGCGG 1 cut(s) 142
SalI GTCGAC 1 cut(s) 2819
SapI GCTCTTC 1 cut(s) 1953
SaqAI TTAA 5 cut(s) 633, 1151, 1500, 1607, 1740
Sau96I GGNCC 7 cut(s) 126, 1342, 2080, 2081, 2377, 2520, 2581
SchI GAGTC 7 cut(s) 45, 138, 557, 740, 1657, 2363, 2634
ScrFI CCNGG 9 cut(s) 33, 124, 187, 205, 538, 791, 860, 879, 2220
SduI GDGCHC 5 cut(s) 212, 1363, 1815, 2084, 2215
SexAI ACCWGGT 1 cut(s) 877
SfaNI GCATC 7 cut(s) 340, 834, 1126, 1342, 1571, 2662, 2787
SfcI CTRYAG 4 cut(s) 672, 1278, 1922, 2907
Sfr274I CTCGAG 1 cut(s) 2028
Sfr303I CCGCGG 1 cut(s) 142
SgrAI CRCCGGYG 1 cut(s) 2087
SgrBI CCGCGG 1 cut(s) 142
SinI GGWCC 3 cut(s) 1342, 2520, 2581
SlaI CTCGAG 1 cut(s) 2028
SmiMI CAYNNNNRTG 3 cut(s) 1971, 2075, 2319
SmlI CTYRAG 7 cut(s) 437, 527, 1043, 1586, 2028, 2782, 2885
SmoI CTYRAG 7 cut(s) 437, 527, 1043, 1586, 2028, 2782, 2885
SphI GCATGC 1 cut(s) 2192
SspI AATATT 1 cut(s) 2602
SspMI CTAG 6 cut(s) 393, 1490, 2064, 2108, 2724, 2870
SstI GAGCTC 1 cut(s) 1815
StyD4I CCNGG 9 cut(s) 31, 122, 185, 203, 536, 789, 858, 877, 2218
StyI CCWWGG 5 cut(s) 234, 318, 765, 2577, 2772
TaiI ACGT 2 cut(s) 1975, 2463
TaqI TCGA 6 cut(s) 162, 1428, 1666, 1815, 2029, 2820
TatI WGTACW 3 cut(s) 1039, 2175, 2559
TauI GCSGC 2 cut(s) 29, 2382
TfiI GAWTC 9 cut(s) 159, 475, 677, 832, 955, 1058, 2545, 2743, 2900
Tru1I TTAA 5 cut(s) 633, 1151, 1500, 1607, 1740
Tru9I TTAA 5 cut(s) 633, 1151, 1500, 1607, 1740
TscAI CASTG 7 cut(s) 1206, 1405, 1515, 1643, 2146, 2389, 2407
TseFI GTSAC 2 cut(s) 1536, 2830
Tsp45I GTSAC 2 cut(s) 1536, 2830
TspGWI ACGGA 6 cut(s) 38, 97, 762, 1591, 2463, 2512
TspRI CASTG 7 cut(s) 1206, 1405, 1515, 1643, 2146, 2389, 2407
Van91I CCANNNNNTGG 2 cut(s) 884, 2578
VneI GTGCAC 1 cut(s) 1359
VpaK11BI GGWCC 3 cut(s) 1342, 2520, 2581
VspI ATTAAT 1 cut(s) 1500
XapI RAATTY 5 cut(s) 638, 1092, 1668, 1774, 2343
XceI RCATGY 2 cut(s) 2134, 2192
XcmI CCANNNNNNNNNTGG 1 cut(s) 1459
XhoI CTCGAG 1 cut(s) 2028
XmiI GTMKAC 1 cut(s) 2820
XmnI GAANNNNTTC 1 cut(s) 2016
XspI CTAG 6 cut(s) 393, 1490, 2064, 2108, 2724, 2870
ZraI GACGTC 1 cut(s) 2461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.