Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008290

Physical Location & Seq

Genomic Coordinates Forward (+)

5333750 .. 5337784

Gene Structure 4035 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G16290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGGCGGCGACGACGACTGCTACTTCATTATTCTCATCTCGTCTTCACTTCCAGAATCAGAATCAAGGTTATGGGTTTCCGGCGAAAACCCCCAACTCCCTGCAAGTAAATCAGATCATCGACGGGAGGAAGATGAGGAATGCCACTGTTCTCTCAGCTGCTTCCACTGATAAAGCCATCACCACTGCTCAATCTGTAGCTCCGACGGCTTGTGATAGAGTAAGGAGGCATACAATCTCGGTGTTTGTGGGCGATGAGAGCGGTATAATAAATAGAATTGCTGGAGTATTTGCTAGAAGGGGATACAATATTGAGTCCCTTGCCGTAGGATTGAATGAAGACAAGGCTCTGTTTACTATAGTTGTTCTTGGAACAGATAAAGTCTTGCAGCAAGTTGTAGAACAGCTTAACAAGCTCGTCAATGTCATCAAGGTGGAAGATCTCTCCAAGGAGCCCCATGTAGAACGTGAGTTAATGCTCATTAAACTTAACGCTGATCCAAGCACCCGCTCAGAGATCATGTGGTTGGTGGATATCTTTAGAGCAAAAATCGTGGATACCTCAGAGCAATCTCTAACGATTGAGGTAACTGGAGACCCTGGAAAAATGGTTGCACTAACGACAAACTTAGAGAAGTTTGGAATAAAAGAAATTGCGAGAACAGGGAAGATTGCTCTGAGACGGGAAAAGATGGGAGAAACCGCTCCGTTTTGGAGATTTTCAGCGGCATCATATCCACATCTTGTAAAGGAATCATCTCATGAGACTGTTGCAGAGAAAACAAAACTAGCATTGACTGGAAATGGCAATGCATCATCCGGGGGTGACGTTTATCCAGTGGAACCCTATAACGATTTCAAACCGGTCCTTGACGCTCATTGGGGAATGGTCTACGATGAAGATTCGAGCGGACTTCGGTCCCACACATTATCTTTACTTGTGGCCAACGTTCCCGGAGTTCTCAATCTAATAACTGGAGCTATCTCTAGAAGAGGTTATAACATCCAGAGTCTAGCTGTTGGTCCTGCTGAGAAAGAGGGCTTATCTCGGATTACAACAGTTATTCCCGGAACTGATGAAAATATAGATAAATTGGTTAGGCAGCTCCAGAAGTTGATTGATCTTCAGGAGATCCAAAATATAACGCACATGCCATTTGCGGAGCGAGAACTGATGCTCATCAAAGTAGCTGCTGATACTTCTGCAAGGAGAGATGTTTTAGATATTGCTCAAGTTTTCCGTGCTAAAGCCATTGATGTCTCTGATCATACTATCACCCTTGAGGTTACAGGGGATCTCAGAAAAATGTCTGCATTACAAACGCAGTTAGAGGCCTATGGAATCTGCGAGGTGGCTCGGACAGGAAGAGTGGCATTGGTAAGAGAATCTGGAGTGGATTCGACTTATCTTCGTGGATACTCTCTTCCTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006551 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

477

Amino Acids

52.33

Weight (kDa)

6.78

Isoelectric Point (pI)

40.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	77 - 140	3e-11	ACT domain
ACT_AHAS_ss	PF22629	79 - 144	5e-17	AHAS small subunit-like ACT domain
ACT_5	PF13710	87 - 145	3.1e-08	ACT domain
ALS_ss_C	PF10369	156 - 229	3.3e-29	Small subunit of acetolactate synthase
ACT	PF01842	308 - 373	8.3e-11	ACT domain
ACT_AHAS_ss	PF22629	310 - 374	1.8e-21	AHAS small subunit-like ACT domain
ACT_5	PF13710	317 - 377	4.3e-08	ACT domain
ALS_ss_C	PF10369	389 - 461	2.5e-26	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16290 AT5G16290
fragaria_vesca	FvH4_3g07270 FvH4_3g07270 FvH4_3g07270
malus_domestica	MD00G1009000.v1.1 MD10G1275300.v1.1
prunus_persica	Prupe.4G067100_v2.0.a1 Prupe.4G067100_v2.0.a1 Prupe.4G067100_v2.0.a1
pyrus_communis	pycom05g27400 pycom10g23000
rosa_chinensis	RchiOBHm_Chr5g0011751
rosa_laevigata	RLG00000031859
rosa_multiflora	Rmu_ssc0000357.1_g000019
rosa_roxburghii	Rroxscaffold_1G00064800
rosa_rugosa	Rorug04G0458600 Rorug04G0458600
rosa_samantha	Rh5AG090400 Rh5BG085900 Rh5CG099700 Rh5DG086100
rosa_wichuraiana	Rw5G007890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	999
AccBSI	CCGCTC	4 cut(s)	261, 510, 704, 909
AccI	GTMKAC	1 cut(s)	891
AciI	CCGC	7 cut(s)	5, 261, 508, 702, 725, 909, 1160
AclI	AACGTT	1 cut(s)	948
AclWI	GGATC	3 cut(s)	491, 1126, 1302
AcoI	YGGCCR	1 cut(s)	942
AcuI	CTGAAG	1 cut(s)	1109
AgeI	ACCGGT	1 cut(s)	862
AgsI	TTSAA	2 cut(s)	334, 859
AjnI	CCWGG	1 cut(s)	598
AluBI	AGCT	8 cut(s)	158, 200, 406, 415, 980, 1016, 1105, 1190
AluI	AGCT	8 cut(s)	158, 200, 406, 415, 980, 1016, 1105, 1190
Alw26I	GTCTC	4 cut(s)	588, 673, 758, 1264
AlwI	GGATC	3 cut(s)	491, 1126, 1302
AoxI	GGCC	2 cut(s)	942, 1332
ApeKI	GCWGC	4 cut(s)	158, 388, 1102, 1190
AsiGI	ACCGGT	1 cut(s)	862
AspS9I	GGNCC	3 cut(s)	865, 918, 1022
AsuC2I	CCSGG	3 cut(s)	820, 954, 1068
AsuHPI	GGTGA	3 cut(s)	172, 836, 1267
AvaII	GGWCC	3 cut(s)	865, 918, 1022
BalI	TGGCCA	1 cut(s)	944
BanII	GRGCYC	1 cut(s)	456
BbsI	GAAGAC	2 cut(s)	35, 345
BbvI	GCAGC	4 cut(s)	145, 400, 1114, 1177
BccI	CCATC	2 cut(s)	185, 685
BceAI	ACGGC	2 cut(s)	222, 308
BciT130I	CCWGG	1 cut(s)	600
BciVI	GTATCC	3 cut(s)	296, 550, 1409
BclI	TGATCA	1 cut(s)	1264
BcnI	CCSGG	3 cut(s)	820, 954, 1068
BcoDI	GTCTC	4 cut(s)	588, 673, 758, 1264
BfaI	CTAG	4 cut(s)	294, 788, 987, 1013
BfmI	CTRYAG	2 cut(s)	195, 357
BfuI	GTATCC	3 cut(s)	296, 550, 1409
BglII	AGATCT	1 cut(s)	439
BisI	GCNGC	6 cut(s)	6, 159, 389, 726, 1103, 1191
BlsI	GCNGC	6 cut(s)	7, 160, 390, 727, 1104, 1192
Bme1390I	CCNGG	4 cut(s)	600, 820, 954, 1068
Bme18I	GGWCC	3 cut(s)	865, 918, 1022
BmgT120I	GGNCC	3 cut(s)	865, 918, 1022
BmiI	GGNNCC	3 cut(s)	453, 843, 920
BmrFI	CCNGG	4 cut(s)	600, 820, 954, 1068
BmsI	GCATC	3 cut(s)	737, 821, 1164
BoxI	GACNNNNGTC	1 cut(s)	916
BpiI	GAAGAC	2 cut(s)	35, 345
BpmI	CTGGAG	5 cut(s)	303, 612, 996, 1091, 1410
BpuEI	CTTGAG	2 cut(s)	1215, 1301
BpuMI	CCSGG	3 cut(s)	820, 954, 1068
BsaBI	GATNNNNATC	1 cut(s)	1178
BsaI	GGTCTC	1 cut(s)	588
BsaJI	CCNNGG	3 cut(s)	447, 598, 819
BsaWI	WCCGGW	1 cut(s)	862
Bse118I	RCCGGY	1 cut(s)	862
Bse1I	ACTGG	4 cut(s)	595, 802, 836, 979
Bse3DI	GCAATG	1 cut(s)	814
Bse8I	GATNNNNATC	1 cut(s)	1178
BseBI	CCWGG	1 cut(s)	600
BseDI	CCNNGG	3 cut(s)	447, 598, 819
BseGI	GGATG	2 cut(s)	815, 1002
BseJI	GATNNNNATC	1 cut(s)	1178
BseMI	GCAATG	1 cut(s)	814
BseMII	CTCAG	6 cut(s)	168, 525, 576, 668, 1020, 1312
BseNI	ACTGG	4 cut(s)	595, 802, 836, 979
BseXI	GCAGC	4 cut(s)	145, 400, 1114, 1177
BshFI	GGCC	2 cut(s)	944, 1334
BshTI	ACCGGT	1 cut(s)	862
BsiSI	CCGG	5 cut(s)	80, 819, 863, 954, 1068
BslFI	GGGAC	2 cut(s)	301, 904
BsmAI	GTCTC	4 cut(s)	588, 673, 758, 1264
BsmBI	CGTCTC	1 cut(s)	673
BsmFI	GGGAC	2 cut(s)	301, 904
BsmI	GAATGC	1 cut(s)	145
BsnI	GGCC	2 cut(s)	944, 1334
Bso31I	GGTCTC	1 cut(s)	588
Bsp1286I	GDGCHC	1 cut(s)	456
Bsp143I	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
BspACI	CCGC	7 cut(s)	5, 261, 508, 702, 725, 909, 1160
BspANI	GGCC	2 cut(s)	944, 1334
BspCNI	CTCAG	6 cut(s)	167, 524, 575, 669, 1021, 1311
BspHI	TCATGA	1 cut(s)	760
BspLI	GGNNCC	3 cut(s)	453, 843, 920
BspPI	GGATC	3 cut(s)	491, 1126, 1302
BspTNI	GGTCTC	1 cut(s)	588
BsrBI	CCGCTC	4 cut(s)	261, 510, 704, 909
BsrDI	GCAATG	1 cut(s)	814
BsrFI	RCCGGY	1 cut(s)	862
BsrI	ACTGG	4 cut(s)	595, 802, 836, 979
BssAI	RCCGGY	1 cut(s)	862
BssECI	CCNNGG	3 cut(s)	447, 598, 819
BssMI	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
BssT1I	CCWWGG	1 cut(s)	447
Bst2UI	CCWGG	1 cut(s)	600
Bst4CI	ACNGT	3 cut(s)	148, 769, 1060
Bst6I	CTCTTC	3 cut(s)	985, 1360, 1428
BstDEI	CTNAG	7 cut(s)	154, 511, 562, 628, 677, 1029, 1298
BstF5I	GGATG	2 cut(s)	815, 1002
BstKTI	GATC	8 cut(s)	117, 442, 499, 519, 1123, 1134, 1267, 1297
BstMAI	GTCTC	4 cut(s)	588, 673, 758, 1264
BstMBI	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
BstMWI	GCNNNNNNNGC	3 cut(s)	206, 258, 412
BstNI	CCWGG	1 cut(s)	600
BstNSI	RCATGY	1 cut(s)	1153
BstPAI	GACNNNNGTC	1 cut(s)	916
BstSCI	CCNGG	4 cut(s)	598, 818, 952, 1066
BstSFI	CTRYAG	2 cut(s)	195, 357
BstV1I	GCAGC	4 cut(s)	145, 400, 1114, 1177
BstV2I	GAAGAC	2 cut(s)	35, 345
BstX2I	RGATCY	3 cut(s)	439, 1131, 1294
BstYI	RGATCY	3 cut(s)	439, 1131, 1294
BsuI	GTATCC	3 cut(s)	296, 550, 1409
BsuRI	GGCC	2 cut(s)	944, 1334
BtgZI	GCGATG	1 cut(s)	267
BtsCI	GGATG	2 cut(s)	815, 1002
BtsI	GCAGTG	1 cut(s)	183
BtsIMutI	CAGTG	4 cut(s)	144, 165, 183, 843
CciI	TCATGA	1 cut(s)	760
Cfr10I	RCCGGY	1 cut(s)	862
Cfr13I	GGNCC	3 cut(s)	865, 918, 1022
CseI	GACGC	1 cut(s)	881
CspAI	ACCGGT	1 cut(s)	862
CspCI	CAANNNNNGTGG	2 cut(s)	534, 569
CviAII	CATG	4 cut(s)	458, 520, 761, 1150
DdeI	CTNAG	7 cut(s)	154, 511, 562, 628, 677, 1029, 1298
DpnI	GATC	8 cut(s)	116, 441, 498, 518, 1122, 1133, 1266, 1296
DpnII	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
EaeI	YGGCCR	1 cut(s)	942
Eam1104I	CTCTTC	3 cut(s)	985, 1360, 1428
EarI	CTCTTC	3 cut(s)	985, 1360, 1428
Eco130I	CCWWGG	1 cut(s)	447
Eco147I	AGGCCT	1 cut(s)	1334
Eco24I	GRGCYC	1 cut(s)	456
Eco31I	GGTCTC	1 cut(s)	588
Eco32I	GATATC	1 cut(s)	535
Eco47I	GGWCC	3 cut(s)	865, 918, 1022
Eco57I	CTGAAG	1 cut(s)	1109
EcoRII	CCWGG	1 cut(s)	598
EcoRV	GATATC	1 cut(s)	535
EcoT14I	CCWWGG	1 cut(s)	447
EcoT22I	ATGCAT	1 cut(s)	814
EcoT38I	GRGCYC	1 cut(s)	456
ErhI	CCWWGG	1 cut(s)	447
Esp3I	CGTCTC	1 cut(s)	673
FaeI	CATG	4 cut(s)	461, 523, 764, 1153
FaqI	GGGAC	2 cut(s)	301, 904
FatI	CATG	4 cut(s)	457, 519, 760, 1149
FauI	CCCGC	1 cut(s)	515
FbaI	TGATCA	1 cut(s)	1264
FblI	GTMKAC	1 cut(s)	891
Fnu4HI	GCNGC	6 cut(s)	6, 159, 389, 726, 1103, 1191
FokI	GGATG	2 cut(s)	802, 989
FriOI	GRGCYC	1 cut(s)	456
Fsp4HI	GCNGC	6 cut(s)	6, 159, 389, 726, 1103, 1191
FspBI	CTAG	4 cut(s)	294, 788, 987, 1013
GluI	GCNGC	6 cut(s)	6, 159, 389, 726, 1103, 1191
GsuI	CTGGAG	5 cut(s)	303, 612, 996, 1091, 1410
HaeIII	GGCC	2 cut(s)	944, 1334
HapII	CCGG	5 cut(s)	80, 819, 863, 954, 1068
HgaI	GACGC	1 cut(s)	881
Hin1II	CATG	4 cut(s)	461, 523, 764, 1153
HinfI	GANTC	9 cut(s)	55, 61, 314, 752, 902, 1009, 1341, 1385, 1397
HpaII	CCGG	5 cut(s)	80, 819, 863, 954, 1068
HphI	GGTGA	3 cut(s)	172, 836, 1267
Hpy166II	GTNNAC	2 cut(s)	354, 892
Hpy188III	TCNNGA	7 cut(s)	52, 761, 987, 1006, 1108, 1127, 1389
Hpy8I	GTNNAC	2 cut(s)	354, 892
Hpy99I	CGWCG	4 cut(s)	13, 16, 125, 208
HpyAV	CCTTC	1 cut(s)	291
HpyCH4III	ACNGT	3 cut(s)	148, 769, 1060
HpyCH4IV	ACGT	3 cut(s)	466, 828, 948
HpyCH4V	TGCA	7 cut(s)	103, 388, 614, 773, 812, 1205, 1313
HpyF10VI	GCNNNNNNNGC	3 cut(s)	206, 258, 412
HpyF3I	CTNAG	7 cut(s)	154, 511, 562, 628, 677, 1029, 1298
HpySE526I	ACGT	3 cut(s)	466, 828, 948
Hsp92II	CATG	4 cut(s)	461, 523, 764, 1153
Ksp22I	TGATCA	1 cut(s)	1264
Kzo9I	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
LmnI	GCTCC	6 cut(s)	205, 451, 709, 977, 1110, 1162
Lsp1109I	GCAGC	4 cut(s)	145, 400, 1114, 1177
LweI	GCATC	3 cut(s)	737, 821, 1164
MaeI	CTAG	4 cut(s)	294, 788, 987, 1013
MaeII	ACGT	3 cut(s)	466, 828, 948
MaeIII	GTNAC	3 cut(s)	586, 824, 1285
MalI	GATC	8 cut(s)	116, 441, 498, 518, 1122, 1133, 1266, 1296
MbiI	CCGCTC	4 cut(s)	261, 510, 704, 909
MboI	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
MflI	RGATCY	3 cut(s)	439, 1131, 1294
MhlI	GDGCHC	1 cut(s)	456
MlsI	TGGCCA	1 cut(s)	944
MluCI	AATT	3 cut(s)	276, 651, 1091
MluNI	TGGCCA	1 cut(s)	944
MlyI	GAGTC	2 cut(s)	323, 1018
MmeI	TCCRAC	1 cut(s)	227
Mox20I	TGGCCA	1 cut(s)	944
Mph1103I	ATGCAT	1 cut(s)	814
MscI	TGGCCA	1 cut(s)	944
MseI	TTAA	4 cut(s)	408, 473, 483, 489
MslI	CAYNNNNRTG	1 cut(s)	431
Msp20I	TGGCCA	1 cut(s)	944
MspA1I	CMGCKG	2 cut(s)	158, 725
MspI	CCGG	5 cut(s)	80, 819, 863, 954, 1068
MspR9I	CCNGG	4 cut(s)	600, 820, 954, 1068
Mva1269I	GAATGC	1 cut(s)	145
MvaI	CCWGG	1 cut(s)	600
MwoI	GCNNNNNNNGC	3 cut(s)	206, 258, 412
NciI	CCSGG	3 cut(s)	820, 954, 1068
NdeII	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
NlaIII	CATG	4 cut(s)	461, 523, 764, 1153
NlaIV	GGNNCC	3 cut(s)	453, 843, 920
NmuCI	GTSAC	1 cut(s)	824
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	1 cut(s)	1153
PagI	TCATGA	1 cut(s)	760
PceI	AGGCCT	1 cut(s)	1334
PctI	GAATGC	1 cut(s)	145
PfeI	GAWTC	7 cut(s)	55, 61, 752, 902, 1341, 1385, 1397
PfoI	TCCNGGA	2 cut(s)	952, 1066
PinAI	ACCGGT	1 cut(s)	862
PkrI	GCNGC	6 cut(s)	7, 160, 390, 727, 1104, 1192
PleI	GAGTC	2 cut(s)	322, 1017
PpsI	GAGTC	2 cut(s)	322, 1017
PshAI	GACNNNNGTC	1 cut(s)	916
PsiI	TTATAA	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	948
Psp6I	CCWGG	1 cut(s)	598
PspGI	CCWGG	1 cut(s)	598
PspN4I	GGNNCC	3 cut(s)	453, 843, 920
PspPI	GGNCC	3 cut(s)	865, 918, 1022
PsuI	RGATCY	3 cut(s)	439, 1131, 1294
PvuII	CAGCTG	1 cut(s)	158
RseI	CAYNNNNRTG	1 cut(s)	431
SaqAI	TTAA	4 cut(s)	408, 473, 483, 489
SatI	GCNGC	6 cut(s)	6, 159, 389, 726, 1103, 1191
Sau3AI	GATC	8 cut(s)	114, 439, 496, 516, 1120, 1131, 1264, 1294
Sau96I	GGNCC	3 cut(s)	865, 918, 1022
SchI	GAGTC	2 cut(s)	323, 1018
ScrFI	CCNGG	4 cut(s)	600, 820, 954, 1068
SduI	GDGCHC	1 cut(s)	456
SfaNI	GCATC	3 cut(s)	737, 821, 1164
SfcI	CTRYAG	2 cut(s)	195, 357
SinI	GGWCC	3 cut(s)	865, 918, 1022
SmiMI	CAYNNNNRTG	1 cut(s)	431
SmlI	CTYRAG	2 cut(s)	1230, 1280
SmoI	CTYRAG	2 cut(s)	1230, 1280
Sse9I	AATT	3 cut(s)	276, 651, 1091
SseBI	AGGCCT	1 cut(s)	1334
SsiI	CCGC	7 cut(s)	5, 261, 508, 702, 725, 909, 1160
SspI	AATATT	1 cut(s)	310
SspMI	CTAG	4 cut(s)	294, 788, 987, 1013
StuI	AGGCCT	1 cut(s)	1334
StyD4I	CCNGG	4 cut(s)	598, 818, 952, 1066
StyI	CCWWGG	1 cut(s)	447
TaaI	ACNGT	3 cut(s)	148, 769, 1060
TaiI	ACGT	3 cut(s)	469, 831, 951
TaqI	TCGA	3 cut(s)	120, 905, 1400
TaqII	GACCGA	1 cut(s)	906
TasI	AATT	3 cut(s)	276, 651, 1091
TauI	GCSGC	2 cut(s)	8, 728
TfiI	GAWTC	7 cut(s)	55, 61, 752, 902, 1341, 1385, 1397
Tru1I	TTAA	4 cut(s)	408, 473, 483, 489
Tru9I	TTAA	4 cut(s)	408, 473, 483, 489
TscAI	CASTG	4 cut(s)	151, 172, 190, 843
TseFI	GTSAC	1 cut(s)	824
TseI	GCWGC	4 cut(s)	158, 388, 1102, 1190
Tsp45I	GTSAC	1 cut(s)	824
TspDTI	ATGAA	4 cut(s)	15, 351, 912, 1092
TspGWI	ACGGA	2 cut(s)	696, 1229
TspRI	CASTG	4 cut(s)	151, 172, 190, 843
VpaK11BI	GGWCC	3 cut(s)	865, 918, 1022
XbaI	TCTAGA	1 cut(s)	986
XceI	RCATGY	1 cut(s)	1153
XmiI	GTMKAC	1 cut(s)	891
XspI	CTAG	4 cut(s)	294, 788, 987, 1013
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.