MD00G1009000.v1.1

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008290
Physical Location & Seq
Forward (+)
1127541 .. 1137322
9782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1009000.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGCGGCGGCTGTGTCATCACCGTGCTCCCCTTCACTAGTGGCCCAGAAACCGAGTTTCCGAGTGGATTTCCGGCATGGGTTTTCCACAAGCTTCGATTTTGGCCGCCAGAGGCCGCTCAAATGGAAGAGTTACGGGCTGAAGAAGCTGGTTGTTTCTGCTAGCACCGGCAATGCTGTTTCTGTCAGTGATTCTGTGCCTTCATTGACTAGTTCTAAGGTCAAGCGCCATACGATTTCAGTGTTTGTTGGGGATGAGAGTGGAATCATAAACCGAATAGCAGGGGTTTTCGCCAGGAGAGGTTACAATATCGAGTCTCTTGCTGTTGGTTTGAACAAGGACAAGGCTCTTTTTACCATAGTTGTGTCTGGAACTGGAAAGGTTTTGCGACAGGTCGTTGAACAGCTTAACAAGTTGGTGAATGTGATAAAGGTCGAAGACATCTCAAGGGAGCCACAAGTAGAACGTGAATTGATGCTTATCAAACTTAATGCGGATCCAAGTACACGTGCAGAGATAATGTGGTTAGTGGACATCTTCAGAGCAAAAATTGTTGATATCTCGGAACAGTCGCTGACAGTTGAGGTTACTGGAGACCCTGGGAAGGTGGTTGCTGTTCAAAGAAACTTAAGCAAGTTTGGAATCAAAGAACTTGCAAGAACTGGAAAGATTGCTTTGAGACGAGAAAAGATGGGTGCGACAGCTCCTTTCTGGAGATTTTCTGCAGCTTCTTATCCAGATCTTGAAAAGTCCACGTCTGATGATGACCTTGCAGTGAAAGCAAATGGTACACTCAATGGTGATGCTGGTACCGCCTCAAGGGGAGATGTTTATCCTGTCGAGCCCTATGATGACTTCCGTTCGAATAAAGTTCTTGATGCTCATTGGGGTGTTCTCTATGATGAAGATTCAAGTGGGGTTCAATCCCACACTTTGTCTATGATTGTGAATGACTCTCCTGGGGTTCTAAACATAGTTACAGGAGTCATATCTCGAAGAGGTTATAACATTCAGAGCCTAGCTGTGGGGCCCGCTGAAAAGGAGGGGCTTTCTCGCATTACAACAGTTGTTCCCGGTACAGAGGATTCAATTTCCAAGTTGGTTCAGCAACTTCACAAGTTAATAGATCTTCATGAGGTTCGGGATATTACTCACTCACCTTTTGCTGAGCGAGAGTTGATGTTGATAAAGATTGCTGTGAACGCTACTGCTAGAAGGGATGTTCTTGATATTGCCAGCATTTTCCGCGCTAAAGCTGTTGATGTATCTGATCACACAGTTACCCTTGAGCTTACTGGTGATTTGAATAAAATGGTTGCACTGCAGAAGTTACTAGAGCCCTACGGGATTTGTGAGGTTGCACGGACTGGGAGGGTGGCGTTGGAGCGGGAGTCTGGTGTGGATTCCACATATCTCCGCGGATACCCTCTTCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

480

Amino Acids

52.33

Weight (kDa)

8.79

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 77 - 140 2.5e-10 ACT domain
ACT_AHAS_ss PF22629 79 - 144 1.1e-16 AHAS small subunit-like ACT domain
ACT_5 PF13710 87 - 145 5.1e-08 ACT domain
ALS_ss_C PF10369 156 - 229 1.7e-28 Small subunit of acetolactate synthase
ACT PF01842 310 - 375 1.1e-10 ACT domain
ACT_AHAS_ss PF22629 312 - 377 4.7e-22 AHAS small subunit-like ACT domain
ACT_5 PF13710 318 - 380 5.3e-11 ACT domain
ALS_ss_C PF10369 391 - 464 6.4e-28 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc65I GGTACC 1 cut(s) 809
AccB1I GGYRCC 1 cut(s) 809
AccBSI CCGCTC 2 cut(s) 118, 1387
AccII CGCG 2 cut(s) 1248, 1419
AclWI GGATC 2 cut(s) 491, 504
AcoI YGGCCR 1 cut(s) 103
AcuI CTGAAG 2 cut(s) 161, 523
AcvI CACGTG 1 cut(s) 509
AfaI GTAC 4 cut(s) 505, 790, 811, 1078
AfiI CCNNNNNNNGG 3 cut(s) 604, 819, 1024
AflII CTTAAG 1 cut(s) 628
AflIII ACRYGT 1 cut(s) 506
AgsI TTSAA 8 cut(s) 334, 401, 620, 746, 912, 923, 1089, 1306
AhlI ACTAGT 2 cut(s) 37, 209
AjiI CACGTC 1 cut(s) 756
AjnI CCWGG 3 cut(s) 293, 598, 958
AluBI AGCT 8 cut(s) 93, 148, 406, 704, 728, 1022, 1256, 1291
AluI AGCT 8 cut(s) 93, 148, 406, 704, 728, 1022, 1256, 1291
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 3 cut(s) 321, 588, 673
AlwI GGATC 2 cut(s) 491, 504
AlwNI CAGNNNCTG 1 cut(s) 574
AoxI GGCC 4 cut(s) 42, 103, 113, 1028
ApaI GGGCCC 1 cut(s) 1032
ApeKI GCWGC 1 cut(s) 725
Asp718I GGTACC 1 cut(s) 809
AspLEI GCGC 2 cut(s) 228, 1250
AspS9I GGNCC 3 cut(s) 43, 1028, 1029
AsuC2I CCSGG 1 cut(s) 1074
AsuHPI GGTGA 5 cut(s) 12, 430, 812, 1149, 1310
AsuII TTCGAA 1 cut(s) 863
AsuNHI GCTAGC 1 cut(s) 161
BaeGI GKGCMC 1 cut(s) 1032
BamHI GGATCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 3 cut(s) 846, 1032, 1341
BbrPI CACGTG 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 737
BccI CCATC 1 cut(s) 685
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 3 cut(s) 295, 600, 960
BciVI GTATCC 1 cut(s) 1415
BclI TGATCA 1 cut(s) 1270
BcnI CCSGG 1 cut(s) 1074
BcoDI GTCTC 3 cut(s) 321, 588, 673
BcuI ACTAGT 2 cut(s) 37, 209
BfaI CTAG 6 cut(s) 38, 162, 210, 1019, 1212, 1334
BfmI CTRYAG 2 cut(s) 723, 1322
BfoI RGCGCY 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 628
BfuI GTATCC 1 cut(s) 1415
BglII AGATCT 2 cut(s) 739, 1126
BisI GCNGC 5 cut(s) 6, 9, 106, 116, 726
BlpI GCTNAGC 1 cut(s) 1167
BlsI GCNGC 5 cut(s) 7, 10, 107, 117, 727
Bme1390I CCNGG 4 cut(s) 295, 600, 960, 1074
BmgBI CACGTC 1 cut(s) 756
BmgT120I GGNCC 3 cut(s) 43, 1028, 1029
BmiI GGNNCC 5 cut(s) 453, 498, 811, 1029, 1030
BmrFI CCNGG 4 cut(s) 295, 600, 960, 1074
BmrI ACTGGG 1 cut(s) 1377
BmsI GCATC 3 cut(s) 465, 793, 868
BmtI GCTAGC 1 cut(s) 165
BmuI ACTGGG 1 cut(s) 1377
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 2 cut(s) 612, 733
Bpu1102I GCTNAGC 1 cut(s) 1167
Bpu14I TTCGAA 1 cut(s) 863
BpuEI CTTGAG 3 cut(s) 430, 802, 1307
BpuMI CCSGG 1 cut(s) 1074
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 2 cut(s) 479, 831
BsaI GGTCTC 1 cut(s) 588
BsaJI CCNNGG 4 cut(s) 598, 599, 959, 1417
BsaXI ACNNNNNCTCC 2 cut(s) 1376, 1406
Bsc4I CCNNNNNNNGG 3 cut(s) 604, 819, 1024
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 5 cut(s) 379, 595, 667, 1300, 1372
Bse3DI GCAATG 1 cut(s) 178
Bse8I GATNNNNATC 2 cut(s) 479, 831
BseBI CCWGG 3 cut(s) 295, 600, 960
BseDI CCNNGG 4 cut(s) 598, 599, 959, 1417
BseGI GGATG 2 cut(s) 259, 1225
BseJI GATNNNNATC 2 cut(s) 479, 831
BseLI CCNNNNNNNGG 3 cut(s) 604, 819, 1024
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 1158
BseNI ACTGG 5 cut(s) 379, 595, 667, 1300, 1372
BseSI GKGCMC 1 cut(s) 1032
BseXI GCAGC 1 cut(s) 737
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 2 cut(s) 1248, 1419
BshFI GGCC 4 cut(s) 44, 105, 115, 1030
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 3 cut(s) 73, 168, 1074
BslI CCNNNNNNNGG 3 cut(s) 604, 819, 1024
BsmAI GTCTC 3 cut(s) 321, 588, 673
BsmBI CGTCTC 1 cut(s) 673
BsnI GGCC 4 cut(s) 44, 105, 115, 1030
Bso31I GGTCTC 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 863
Bsp120I GGGCCC 1 cut(s) 1028
Bsp1286I GDGCHC 4 cut(s) 29, 846, 1032, 1341
Bsp143I GATC 4 cut(s) 496, 739, 1126, 1270
Bsp1720I GCTNAGC 1 cut(s) 1167
BspANI GGCC 4 cut(s) 44, 105, 115, 1030
BspCNI CTCAG 1 cut(s) 1159
BspFNI CGCG 2 cut(s) 1248, 1419
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 5 cut(s) 453, 498, 811, 1029, 1030
BspMAI CTGCAG 2 cut(s) 727, 1326
BspOI GCTAGC 1 cut(s) 165
BspPI GGATC 2 cut(s) 491, 504
BspT104I TTCGAA 1 cut(s) 863
BspT107I GGYRCC 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 588
BsrBI CCGCTC 2 cut(s) 118, 1387
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 5 cut(s) 379, 595, 667, 1300, 1372
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 598, 599, 959, 1417
BssMI GATC 4 cut(s) 496, 739, 1126, 1270
Bst2UI CCWGG 3 cut(s) 295, 600, 960
Bst4CI ACNGT 5 cut(s) 24, 570, 580, 1066, 1279
Bst6I CTCTTC 3 cut(s) 122, 991, 1434
BstAFI CTTAAG 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 163, 1032, 1237
BstDEI CTNAG 2 cut(s) 216, 1167
BstDSI CCRYGG 1 cut(s) 1417
BstF5I GGATG 2 cut(s) 259, 1225
BstFNI CGCG 2 cut(s) 1248, 1419
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 2 cut(s) 228, 1250
BstKTI GATC 4 cut(s) 499, 742, 1129, 1273
BstMAI GTCTC 3 cut(s) 321, 588, 673
BstMBI GATC 4 cut(s) 496, 739, 1126, 1270
BstMWI GCNNNNNNNGC 5 cut(s) 145, 779, 812, 1202, 1245
BstNI CCWGG 3 cut(s) 295, 600, 960
BstSCI CCNGG 4 cut(s) 293, 598, 958, 1072
BstSFI CTRYAG 2 cut(s) 723, 1322
BstSLI GKGCMC 1 cut(s) 1032
BstUI CGCG 2 cut(s) 1248, 1419
BstV1I GCAGC 1 cut(s) 737
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 3 cut(s) 496, 739, 1126
BstYI RGATCY 3 cut(s) 496, 739, 1126
BsuI GTATCC 1 cut(s) 1415
BsuRI GGCC 4 cut(s) 44, 105, 115, 1030
BtgI CCRYGG 1 cut(s) 1417
BtrI CACGTC 1 cut(s) 756
BtsCI GGATG 2 cut(s) 259, 1225
BtsI GCAGTG 2 cut(s) 780, 1319
BtsIMutI CAGTG 4 cut(s) 193, 246, 780, 1319
Cac8I GCNNGC 3 cut(s) 163, 1032, 1237
CaiI CAGNNNCTG 1 cut(s) 574
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 2 cut(s) 228, 1250
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 43, 1028, 1029
Cfr42I CCGCGG 1 cut(s) 1420
Csp6I GTAC 4 cut(s) 504, 789, 810, 1077
CviAII CATG 2 cut(s) 77, 1133
CviQI GTAC 4 cut(s) 504, 789, 810, 1077
DdeI CTNAG 2 cut(s) 216, 1167
DpnI GATC 4 cut(s) 498, 741, 1128, 1272
DpnII GATC 4 cut(s) 496, 739, 1126, 1270
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 3 cut(s) 122, 991, 1434
EarI CTCTTC 3 cut(s) 122, 991, 1434
Eco24I GRGCYC 3 cut(s) 846, 1032, 1341
Eco31I GGTCTC 1 cut(s) 588
Eco32I GATATC 1 cut(s) 559
Eco57I CTGAAG 2 cut(s) 161, 523
Eco72I CACGTG 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 1028
EcoRII CCWGG 3 cut(s) 293, 598, 958
EcoRV GATATC 1 cut(s) 559
EcoT38I GRGCYC 3 cut(s) 846, 1032, 1341
Esp3I CGTCTC 1 cut(s) 673
FaeI CATG 2 cut(s) 80, 1136
FatI CATG 2 cut(s) 76, 1132
FauI CCCGC 2 cut(s) 1039, 1380
FbaI TGATCA 1 cut(s) 1270
Fnu4HI GCNGC 5 cut(s) 6, 9, 106, 116, 726
FokI GGATG 2 cut(s) 266, 1232
FriOI GRGCYC 3 cut(s) 846, 1032, 1341
Fsp4HI GCNGC 5 cut(s) 6, 9, 106, 116, 726
FspBI CTAG 6 cut(s) 38, 162, 210, 1019, 1212, 1334
GlaI GCGC 2 cut(s) 227, 1249
GluI GCNGC 5 cut(s) 6, 9, 106, 116, 726
GsuI CTGGAG 2 cut(s) 612, 733
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 4 cut(s) 44, 105, 115, 1030
HapII CCGG 3 cut(s) 73, 168, 1074
HhaI GCGC 2 cut(s) 228, 1250
Hin1II CATG 2 cut(s) 80, 1136
Hin6I GCGC 2 cut(s) 226, 1248
HinP1I GCGC 2 cut(s) 226, 1248
HindIII AAGCTT 1 cut(s) 91
HpaII CCGG 3 cut(s) 73, 168, 1074
HphI GGTGA 5 cut(s) 12, 430, 812, 1149, 1310
Hpy166II GTNNAC 5 cut(s) 506, 532, 753, 791, 1201
Hpy188I TCNGA 6 cut(s) 62, 542, 565, 760, 1014, 1270
Hpy188III TCNNGA 9 cut(s) 369, 712, 737, 743, 875, 993, 1133, 1142, 1226
Hpy8I GTNNAC 5 cut(s) 506, 532, 753, 791, 1201
HpyAV CCTTC 4 cut(s) 42, 210, 598, 1209
HpyCH4III ACNGT 5 cut(s) 24, 570, 580, 1066, 1279
HpyCH4IV ACGT 3 cut(s) 466, 508, 755
HpyCH4V TGCA 7 cut(s) 512, 656, 725, 773, 1319, 1324, 1361
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 779, 812, 1202, 1245
HpyF3I CTNAG 2 cut(s) 216, 1167
HpySE526I ACGT 3 cut(s) 466, 508, 755
Hsp92II CATG 2 cut(s) 80, 1136
HspAI GCGC 2 cut(s) 226, 1248
KpnI GGTACC 1 cut(s) 813
Ksp22I TGATCA 1 cut(s) 1270
KspI CCGCGG 1 cut(s) 1420
Kzo9I GATC 4 cut(s) 496, 739, 1126, 1270
LmnI GCTCC 4 cut(s) 32, 451, 709, 1384
Lsp1109I GCAGC 1 cut(s) 737
LweI GCATC 3 cut(s) 465, 793, 868
MaeI CTAG 6 cut(s) 38, 162, 210, 1019, 1212, 1334
MaeII ACGT 3 cut(s) 466, 508, 755
MaeIII GTNAC 6 cut(s) 131, 302, 586, 976, 1279, 1329
MalI GATC 4 cut(s) 498, 741, 1128, 1272
MbiI CCGCTC 2 cut(s) 118, 1387
MboI GATC 4 cut(s) 496, 739, 1126, 1270
MboII GAAGA 8 cut(s) 139, 154, 449, 529, 917, 1008, 1121, 1421
MflI RGATCY 3 cut(s) 496, 739, 1126
MhlI GDGCHC 4 cut(s) 29, 846, 1032, 1341
MluCI AATT 3 cut(s) 470, 549, 1089
MlyI GAGTC 4 cut(s) 323, 947, 993, 1400
MmeI TCCRAC 1 cut(s) 1362
MseI TTAA 4 cut(s) 408, 489, 629, 1121
MslI CAYNNNNRTG 3 cut(s) 22, 362, 888
MspA1I CMGCKG 2 cut(s) 1034, 1419
MspCI CTTAAG 1 cut(s) 628
MspI CCGG 3 cut(s) 73, 168, 1074
MspR9I CCNGG 4 cut(s) 295, 600, 960, 1074
MvaI CCWGG 3 cut(s) 295, 600, 960
MvnI CGCG 2 cut(s) 1248, 1419
MwoI GCNNNNNNNGC 5 cut(s) 145, 779, 812, 1202, 1245
NciI CCSGG 1 cut(s) 1074
NdeII GATC 4 cut(s) 496, 739, 1126, 1270
NheI GCTAGC 1 cut(s) 161
NlaIII CATG 2 cut(s) 80, 1136
NlaIV GGNNCC 5 cut(s) 453, 498, 811, 1029, 1030
NspV TTCGAA 1 cut(s) 863
PagI TCATGA 1 cut(s) 1132
PasI CCCWGGG 1 cut(s) 599
PfeI GAWTC 6 cut(s) 191, 264, 642, 908, 1085, 1403
PkrI GCNGC 5 cut(s) 7, 10, 107, 117, 727
PleI GAGTC 4 cut(s) 322, 947, 992, 1399
PmaCI CACGTG 1 cut(s) 509
PmlI CACGTG 1 cut(s) 509
PpsI GAGTC 4 cut(s) 322, 947, 992, 1399
Ppu21I YACGTR 1 cut(s) 509
PsiI TTATAA 1 cut(s) 1005
Psp6I CCWGG 3 cut(s) 293, 598, 958
PspCI CACGTG 1 cut(s) 509
PspGI CCWGG 3 cut(s) 293, 598, 958
PspN4I GGNNCC 5 cut(s) 453, 498, 811, 1029, 1030
PspOMI GGGCCC 1 cut(s) 1028
PspPI GGNCC 3 cut(s) 43, 1028, 1029
PstI CTGCAG 2 cut(s) 727, 1326
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 3 cut(s) 496, 739, 1126
RsaI GTAC 4 cut(s) 505, 790, 811, 1078
RsaNI GTAC 4 cut(s) 504, 789, 810, 1077
RseI CAYNNNNRTG 3 cut(s) 22, 362, 888
SacII CCGCGG 1 cut(s) 1420
SaqAI TTAA 4 cut(s) 408, 489, 629, 1121
SatI GCNGC 5 cut(s) 6, 9, 106, 116, 726
Sau3AI GATC 4 cut(s) 496, 739, 1126, 1270
Sau96I GGNCC 3 cut(s) 43, 1028, 1029
SchI GAGTC 4 cut(s) 323, 947, 993, 1400
ScrFI CCNGG 4 cut(s) 295, 600, 960, 1074
SduI GDGCHC 4 cut(s) 29, 846, 1032, 1341
SfaNI GCATC 3 cut(s) 465, 793, 868
SfcI CTRYAG 2 cut(s) 723, 1322
Sfr303I CCGCGG 1 cut(s) 1420
SfuI TTCGAA 1 cut(s) 863
SgrBI CCGCGG 1 cut(s) 1420
SmiMI CAYNNNNRTG 3 cut(s) 22, 362, 888
SmlI CTYRAG 4 cut(s) 445, 628, 817, 1286
SmoI CTYRAG 4 cut(s) 445, 628, 817, 1286
SpeI ACTAGT 2 cut(s) 37, 209
Sse9I AATT 3 cut(s) 470, 549, 1089
SspMI CTAG 6 cut(s) 38, 162, 210, 1019, 1212, 1334
StyD4I CCNGG 4 cut(s) 293, 598, 958, 1072
TaaI ACNGT 5 cut(s) 24, 570, 580, 1066, 1279
TaiI ACGT 3 cut(s) 469, 511, 758
TaqI TCGA 6 cut(s) 96, 312, 435, 840, 863, 994
TasI AATT 3 cut(s) 470, 549, 1089
TatI WGTACW 1 cut(s) 503
TauI GCSGC 4 cut(s) 8, 11, 108, 118
TfiI GAWTC 6 cut(s) 191, 264, 642, 908, 1085, 1403
Tru1I TTAA 4 cut(s) 408, 489, 629, 1121
Tru9I TTAA 4 cut(s) 408, 489, 629, 1121
TscAI CASTG 4 cut(s) 193, 246, 780, 1326
TseI GCWGC 1 cut(s) 725
TspDTI ATGAA 3 cut(s) 192, 918, 1121
TspGWI ACGGA 2 cut(s) 848, 1378
TspRI CASTG 4 cut(s) 193, 246, 780, 1326
Vha464I CTTAAG 1 cut(s) 628
XspI CTAG 6 cut(s) 38, 162, 210, 1019, 1212, 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.