FvH4_3g07270

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008290
Physical Location & Seq
Forward (+)
4324381 .. 4330251
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07270.t1

Sequence Viewer

Length: 1440 bp
ATGGCGGCTTCTCTGTCATCACCACCGTGCTCAACTTCACTATTCACCCCGAACCCATGTTTCCGAGTCGATTTGGGACGTGGGTTCCGGAAAAGTTTAGACCTTGGTCATCAGAGGCCACTAAAGTTCAAGTCTTTGGCGTCTAAGGACCTGGTGGTCTCTGCTAGCACCAACAATGCTGTGTCTGTCACTGACCCTGCGCCTTCATTGATTCGCTCTAAGGTGAAACGCCATACAATTTCAGTGTTTGTTGGTGATGAGAGTGGAATTATAAACCGAATAGCAGGGGTTTTTGCCAGGAGGGGTTACAATATTGAGTCCCTTGCTGTTGGCTTGAACAAGGACAGGGCTCTCTTTACCATAGTTGTCTCAGGGACTGAAAAGATTTTGCAGCAAGTTGTTGAACAGCTCAACAAGTTGGTGAATGTGATAAAGGTTGAAGATATCTCAAGGGAGCCACAAGTAGAACGTGAATTGATGCTTATCAAACTTCATGCAGATCCAAGCAACCGTGCAGAGATCATGTGGTTAGTGGACATCTTCAGAGCAAAAATTGTGGACATCTCTGAACACTCACTAACAATTGAGGTTACTGGAGACCCTGGGAAAATGGTTGCTGTTCAAAGAAACTTTAGCAAATTTGGAATCAAAGAACTTGCAAGAACTGGAAAGATTGCTTTGAGACGAGAAAAGATGGGTGAGACGGCTCCTTTTTGGAGATTTTCTGCAGCTTCTTATCCTGATCTTGAAAGAATGTCTGATGATGAAGTTGCGGTGAAAGCAAATCATTCAGCCAATGGTTCTAGCAGTATTGCCTCACAGGGTGATGTTTATCCAGTGGAGCCATATGATGACTGTCCTTCAAATCAAGTACTTGATGCTCATTGGGGTGTGCTCTATGATGAAGATTCAAGTGGTGTTCAATCACACACTTTGTGTATGCTGGTGAGTGATGTACCTGGAGTCCTGAACATAGTAACAGGAGTCATATCTCGAAGAGGTTATAACATTCAGAGTTTAGCTGTGGGACCTTCTGAGAAGGAAGGGCTTTCTCGCATTACAACTGTTGTTCCTGGTACTGATGACACAATTACCAAGTTGGTCCAGCAACTTCGCAAGTTAATAGATCTTCATGAGGTTCGGGATATTACACACTTGCCATTTGCTGAGCGAGAGTTGATGTTAATAAAGATTGCTGTAAATGCTAATGCTAGAAGGGATGTTCTCGATATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACAATTACTCTTGAGCTTACCGGCGATTTGAATAAAATGGTTGCATTGCAGAAGTTACTAGAGCCCTATGGGCTTTGTGAGATTGCGCGGACTGGGAGAGTGGCATTAGAGCGGGGTTCTGGTGTGGATTCCACTTCTCTCCGTGGATACCCCCTTCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

480

Amino Acids

52.53

Weight (kDa)

7.15

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 78 - 141 4.7e-11 ACT domain
ACT_AHAS_ss PF22629 80 - 145 7.4e-17 AHAS small subunit-like ACT domain
ACT_5 PF13710 88 - 146 4.1e-09 ACT domain
ALS_ss_C PF10369 157 - 230 1.1e-28 Small subunit of acetolactate synthase
ACT_AHAS_ss PF22629 313 - 377 2.8e-21 AHAS small subunit-like ACT domain
ACT PF01842 315 - 375 1.4e-09 ACT domain
ACT_5 PF13710 319 - 380 2.3e-10 ACT domain
ALS_ss_C PF10369 391 - 464 3.5e-27 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 272, 1005, 1438
AasI GACNNNNNNGTC 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 1387
AccII CGCG 1 cut(s) 1363
AccIII TCCGGA 1 cut(s) 87
AciI CCGC 4 cut(s) 5, 773, 1363, 1387
AclWI GGATC 1 cut(s) 494
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 140
AdeI CACNNNGTG 2 cut(s) 824, 936
AfaI GTAC 3 cut(s) 873, 957, 1078
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 5 cut(s) 150, 296, 601, 958, 1072
AleI CACNNNNGTG 1 cut(s) 25
AluBI AGCT 4 cut(s) 409, 731, 1022, 1291
AluI AGCT 4 cut(s) 409, 731, 1022, 1291
Alw21I GWGCWC 2 cut(s) 32, 897
Alw26I GTCTC 5 cut(s) 163, 373, 591, 676, 695
AlwI GGATC 1 cut(s) 494
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 391, 728
ApoI RAATTY 1 cut(s) 638
AspLEI GCGC 2 cut(s) 202, 1363
AspS9I GGNCC 3 cut(s) 148, 1028, 1102
AsuHPI GGTGA 9 cut(s) 12, 37, 235, 266, 433, 710, 787, 836, 958
AsuNHI GCTAGC 1 cut(s) 164
AvaII GGWCC 3 cut(s) 148, 1028, 1102
BanII GRGCYC 2 cut(s) 352, 1341
Bbv12I GWGCWC 2 cut(s) 32, 897
BbvI GCAGC 2 cut(s) 403, 740
BccI CCATC 1 cut(s) 688
BceAI ACGGC 2 cut(s) 720, 1241
BciT130I CCWGG 5 cut(s) 152, 298, 603, 960, 1074
BciVI GTATCC 1 cut(s) 1415
BclI TGATCA 1 cut(s) 1270
BcoDI GTCTC 5 cut(s) 163, 373, 591, 676, 695
BfaI CTAG 4 cut(s) 165, 804, 1212, 1334
BfmI CTRYAG 1 cut(s) 726
BfuI GTATCC 1 cut(s) 1415
BglI GCCNNNNNGGC 1 cut(s) 1345
BglII AGATCT 1 cut(s) 1126
BisI GCNGC 3 cut(s) 6, 392, 729
BlpI GCTNAGC 1 cut(s) 1167
BlsI GCNGC 3 cut(s) 7, 393, 730
BmcAI AGTACT 1 cut(s) 873
Bme1390I CCNGG 5 cut(s) 152, 298, 603, 960, 1074
Bme18I GGWCC 3 cut(s) 148, 1028, 1102
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 3 cut(s) 148, 1028, 1102
BmiI GGNNCC 5 cut(s) 86, 456, 708, 843, 1029
BmrFI CCNGG 5 cut(s) 152, 298, 603, 960, 1074
BmrI ACTGGG 1 cut(s) 1377
BmsI GCATC 2 cut(s) 468, 868
BmtI GCTAGC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 1377
BoxI GACNNNNGTC 1 cut(s) 105
BpmI CTGGAG 2 cut(s) 615, 981
Bpu1102I GCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 2 cut(s) 433, 1307
BsaBI GATNNNNATC 2 cut(s) 482, 831
BsaHI GRCGYC 1 cut(s) 140
BsaI GGTCTC 2 cut(s) 163, 591
BsaJI CCNNGG 4 cut(s) 103, 601, 602, 1417
BsaWI WCCGGW 1 cut(s) 87
Bse118I RCCGGY 1 cut(s) 1295
Bse1I ACTGG 4 cut(s) 598, 670, 836, 1372
Bse3DI GCAATG 1 cut(s) 1319
Bse8I GATNNNNATC 2 cut(s) 482, 831
BseAI TCCGGA 1 cut(s) 87
BseBI CCWGG 5 cut(s) 152, 298, 603, 960, 1074
BseDI CCNNGG 4 cut(s) 103, 601, 602, 1417
BseGI GGATG 1 cut(s) 1225
BseJI GATNNNNATC 2 cut(s) 482, 831
BseMI GCAATG 1 cut(s) 1319
BseMII CTCAG 3 cut(s) 384, 1026, 1158
BseNI ACTGG 4 cut(s) 598, 670, 836, 1372
BseXI GCAGC 2 cut(s) 403, 740
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 1363
BshFI GGCC 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 32, 897
BsiSI CCGG 2 cut(s) 88, 1296
BslFI GGGAC 4 cut(s) 90, 304, 388, 1041
BsmAI GTCTC 5 cut(s) 163, 373, 591, 676, 695
BsmBI CGTCTC 2 cut(s) 676, 695
BsmFI GGGAC 4 cut(s) 90, 304, 388, 1041
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 2 cut(s) 163, 591
Bsp1286I GDGCHC 4 cut(s) 32, 352, 897, 1341
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 5 cut(s) 499, 519, 742, 1126, 1270
Bsp1720I GCTNAGC 1 cut(s) 1167
BspACI CCGC 4 cut(s) 5, 773, 1363, 1387
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 3 cut(s) 383, 1027, 1159
BspEI TCCGGA 1 cut(s) 87
BspFNI CGCG 1 cut(s) 1363
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 5 cut(s) 86, 456, 708, 843, 1029
BspMAI CTGCAG 1 cut(s) 730
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 1 cut(s) 494
BspTNI GGTCTC 2 cut(s) 163, 591
BsrBI CCGCTC 1 cut(s) 1387
BsrDI GCAATG 1 cut(s) 1319
BsrFI RCCGGY 1 cut(s) 1295
BsrI ACTGG 4 cut(s) 598, 670, 836, 1372
BssAI RCCGGY 1 cut(s) 1295
BssECI CCNNGG 4 cut(s) 103, 601, 602, 1417
BssMI GATC 5 cut(s) 499, 519, 742, 1126, 1270
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 5 cut(s) 152, 298, 603, 960, 1074
Bst4CI ACNGT 4 cut(s) 27, 512, 857, 1066
Bst6I CTCTTC 1 cut(s) 991
BstACI GRCGYC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 166, 1237
BstDEI CTNAG 5 cut(s) 144, 219, 370, 1035, 1167
BstDSI CCRYGG 1 cut(s) 1417
BstF5I GGATG 1 cut(s) 1225
BstFNI CGCG 1 cut(s) 1363
BstHHI GCGC 2 cut(s) 202, 1363
BstKTI GATC 5 cut(s) 502, 522, 745, 1129, 1273
BstMAI GTCTC 5 cut(s) 163, 373, 591, 676, 695
BstMBI GATC 5 cut(s) 499, 519, 742, 1126, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 779, 1202, 1345
BstNI CCWGG 5 cut(s) 152, 298, 603, 960, 1074
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 5 cut(s) 150, 296, 601, 958, 1072
BstSFI CTRYAG 1 cut(s) 726
BstUI CGCG 1 cut(s) 1363
BstV1I GCAGC 2 cut(s) 403, 740
BstX2I RGATCY 2 cut(s) 499, 1126
BstYI RGATCY 2 cut(s) 499, 1126
BsuI GTATCC 1 cut(s) 1415
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 1417
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 1225
BtsIMutI CAGTG 3 cut(s) 189, 249, 843
Cac8I GCNNGC 2 cut(s) 166, 1237
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 2 cut(s) 202, 1363
Cfr10I RCCGGY 1 cut(s) 1295
Cfr13I GGNCC 3 cut(s) 148, 1028, 1102
CseI GACGC 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 3 cut(s) 872, 956, 1077
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 4 cut(s) 57, 494, 523, 1133
CviQI GTAC 3 cut(s) 872, 956, 1077
DdeI CTNAG 5 cut(s) 144, 219, 370, 1035, 1167
DpnI GATC 5 cut(s) 501, 521, 744, 1128, 1272
DpnII GATC 5 cut(s) 499, 519, 742, 1126, 1270
DraIII CACNNNGTG 2 cut(s) 824, 936
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 991
EarI CTCTTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 352, 1341
Eco31I GGTCTC 2 cut(s) 163, 591
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 3 cut(s) 148, 1028, 1102
Eco57I CTGAAG 1 cut(s) 526
EcoO109I RGGNCCY 2 cut(s) 148, 1028
EcoRII CCWGG 5 cut(s) 150, 296, 601, 958, 1072
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 2 cut(s) 352, 1341
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 2 cut(s) 676, 695
FaeI CATG 4 cut(s) 60, 497, 526, 1136
FaqI GGGAC 4 cut(s) 90, 304, 388, 1041
FatI CATG 4 cut(s) 56, 493, 522, 1132
FauI CCCGC 1 cut(s) 1380
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 1270
Fnu4HI GCNGC 3 cut(s) 6, 392, 729
FokI GGATG 1 cut(s) 1232
FriOI GRGCYC 2 cut(s) 352, 1341
Fsp4HI GCNGC 3 cut(s) 6, 392, 729
FspBI CTAG 4 cut(s) 165, 804, 1212, 1334
GlaI GCGC 2 cut(s) 201, 1362
GluI GCNGC 3 cut(s) 6, 392, 729
GsuI CTGGAG 2 cut(s) 615, 981
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 88, 1296
HgaI GACGC 1 cut(s) 129
HhaI GCGC 2 cut(s) 202, 1363
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 4 cut(s) 60, 497, 526, 1136
Hin6I GCGC 2 cut(s) 200, 1361
HinP1I GCGC 2 cut(s) 200, 1361
HinfI GANTC 8 cut(s) 66, 211, 317, 645, 908, 963, 984, 1403
HpaII CCGG 2 cut(s) 88, 1296
HphI GGTGA 9 cut(s) 12, 37, 235, 266, 433, 710, 787, 836, 958
Hpy166II GTNNAC 2 cut(s) 535, 559
Hpy188I TCNGA 8 cut(s) 65, 114, 545, 568, 760, 1014, 1036, 1270
Hpy188III TCNNGA 9 cut(s) 88, 740, 746, 967, 993, 1133, 1142, 1226, 1286
Hpy8I GTNNAC 2 cut(s) 535, 559
HpyAV CCTTC 7 cut(s) 213, 870, 1033, 1037, 1041, 1209, 1439
HpyCH4III ACNGT 4 cut(s) 27, 512, 857, 1066
HpyCH4IV ACGT 2 cut(s) 79, 469
HpyCH4V TGCA 7 cut(s) 391, 497, 515, 659, 728, 1319, 1324
HpyF10VI GCNNNNNNNGC 3 cut(s) 779, 1202, 1345
HpyF3I CTNAG 5 cut(s) 144, 219, 370, 1035, 1167
HpySE526I ACGT 2 cut(s) 79, 469
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 4 cut(s) 60, 497, 526, 1136
HspAI GCGC 2 cut(s) 200, 1361
Kpn2I TCCGGA 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 1270
Kzo9I GATC 5 cut(s) 499, 519, 742, 1126, 1270
LmnI GCTCC 3 cut(s) 454, 712, 841
Lsp1109I GCAGC 2 cut(s) 403, 740
LweI GCATC 2 cut(s) 468, 868
MabI ACCWGGT 1 cut(s) 150
MaeI CTAG 4 cut(s) 165, 804, 1212, 1334
MaeII ACGT 2 cut(s) 79, 469
MaeIII GTNAC 5 cut(s) 187, 305, 589, 976, 1329
MalI GATC 5 cut(s) 501, 521, 744, 1128, 1272
MbiI CCGCTC 1 cut(s) 1387
MboI GATC 5 cut(s) 499, 519, 742, 1126, 1270
MboII GAAGA 5 cut(s) 452, 532, 917, 1008, 1121
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 2 cut(s) 499, 1126
MhlI GDGCHC 4 cut(s) 32, 352, 897, 1341
MluCI AATT 8 cut(s) 237, 267, 473, 552, 582, 638, 1089, 1278
MlyI GAGTC 4 cut(s) 75, 326, 972, 993
MnlI CCTC 6 cut(s) 108, 294, 580, 826, 992, 1129
MroI TCCGGA 1 cut(s) 87
MseI TTAA 2 cut(s) 1121, 1184
MslI CAYNNNNRTG 2 cut(s) 25, 888
MspI CCGG 2 cut(s) 88, 1296
MspR9I CCNGG 5 cut(s) 152, 298, 603, 960, 1074
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 5 cut(s) 152, 298, 603, 960, 1074
MvnI CGCG 1 cut(s) 1363
MwoI GCNNNNNNNGC 3 cut(s) 779, 1202, 1345
NdeI CATATG 1 cut(s) 847
NdeII GATC 5 cut(s) 499, 519, 742, 1126, 1270
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 4 cut(s) 60, 497, 526, 1136
NlaIV GGNNCC 5 cut(s) 86, 456, 708, 843, 1029
NmuCI GTSAC 1 cut(s) 187
OliI CACNNNNGTG 1 cut(s) 25
PagI TCATGA 1 cut(s) 1132
PasI CCCWGGG 1 cut(s) 602
PfeI GAWTC 4 cut(s) 211, 645, 908, 1403
PkrI GCNGC 3 cut(s) 7, 393, 730
PleI GAGTC 4 cut(s) 74, 325, 971, 992
PpsI GAGTC 4 cut(s) 74, 325, 971, 992
PpuMI RGGWCCY 2 cut(s) 148, 1028
PshAI GACNNNNGTC 1 cut(s) 105
PsiI TTATAA 3 cut(s) 272, 1005, 1438
Psp5II RGGWCCY 2 cut(s) 148, 1028
Psp6I CCWGG 5 cut(s) 150, 296, 601, 958, 1072
PspGI CCWGG 5 cut(s) 150, 296, 601, 958, 1072
PspN4I GGNNCC 5 cut(s) 86, 456, 708, 843, 1029
PspPI GGNCC 3 cut(s) 148, 1028, 1102
PspPPI RGGWCCY 2 cut(s) 148, 1028
PstI CTGCAG 1 cut(s) 730
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 499, 1126
RsaI GTAC 3 cut(s) 873, 957, 1078
RsaNI GTAC 3 cut(s) 872, 956, 1077
RseI CAYNNNNRTG 2 cut(s) 25, 888
SaqAI TTAA 2 cut(s) 1121, 1184
SatI GCNGC 3 cut(s) 6, 392, 729
Sau3AI GATC 5 cut(s) 499, 519, 742, 1126, 1270
Sau96I GGNCC 3 cut(s) 148, 1028, 1102
ScaI AGTACT 1 cut(s) 873
SchI GAGTC 4 cut(s) 75, 326, 972, 993
ScrFI CCNGG 5 cut(s) 152, 298, 603, 960, 1074
SduI GDGCHC 4 cut(s) 32, 352, 897, 1341
SexAI ACCWGGT 1 cut(s) 150
SfaNI GCATC 2 cut(s) 468, 868
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 3 cut(s) 148, 1028, 1102
SmiMI CAYNNNNRTG 2 cut(s) 25, 888
SmlI CTYRAG 2 cut(s) 448, 1286
SmoI CTYRAG 2 cut(s) 448, 1286
Sse9I AATT 8 cut(s) 237, 267, 473, 552, 582, 638, 1089, 1278
SsiI CCGC 4 cut(s) 5, 773, 1363, 1387
SspI AATATT 1 cut(s) 313
SspMI CTAG 4 cut(s) 165, 804, 1212, 1334
StyD4I CCNGG 5 cut(s) 150, 296, 601, 958, 1072
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 4 cut(s) 27, 512, 857, 1066
TaiI ACGT 2 cut(s) 82, 472
TaqI TCGA 3 cut(s) 69, 994, 1227
TasI AATT 8 cut(s) 237, 267, 473, 552, 582, 638, 1089, 1278
TatI WGTACW 1 cut(s) 871
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 211, 645, 908, 1403
Tru1I TTAA 2 cut(s) 1121, 1184
Tru9I TTAA 2 cut(s) 1121, 1184
TscAI CASTG 3 cut(s) 196, 249, 843
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 391, 728
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 5 cut(s) 195, 482, 780, 918, 1121
TspGWI ACGGA 2 cut(s) 1235, 1406
TspRI CASTG 3 cut(s) 196, 249, 843
VpaK11BI GGWCC 3 cut(s) 148, 1028, 1102
XapI RAATTY 1 cut(s) 638
XspI CTAG 4 cut(s) 165, 804, 1212, 1334
ZrmI AGTACT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.