MD10G1275300.v1.1

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008290
Physical Location & Seq
Forward (+)
36663990 .. 36669660
5671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1275300.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGCGGCTGTGTCATCACCATTCTCCACTTCACTAGTGGCCCAGAAACCGAGTTTCCCAGTGGATTTCCGGCATGGGTTTTCCACAAGCTTCGATCTTGGCCGCCAGAGGCCGCTCAAAGGGAAGAGTTCCGAGCTGAAGAAGCTGGTGATCTCTGCTAGCACTGGCAATGCTGTTTCTGTCAGTGATTCTGTGCCTTCATTGACTTCTAAGGTAAAACGCCATACTATTTCAGTGTTTGTTGGGGATGAGAGTGGAATCATAAACCGAATAGCAGGGGTTTTCGCCAGGAGAGGTTACAATATCGAGTCTCTTGCTGTTGGTTTGAACAAGGACAAGGCTCTCTTTACCATAGTTGTGTCTGGGACTGAAAAGGTGTTGAGGCAGGTTGTTGAACAGCTGAACAAGTTGGTGAATGTGATAAAGGTCGAAGACATCTCAAGGGAGCCACAAGTAGAACGTGAACTGATGCTTATCAAACTTAATGCAGATCCAAGTACCCGTGCAGAGGATGTTGTTGTGTTCTGCATTGGTATTGCAGGCTGGTTAACTTCTTGTTTGATGATAATGTGGTTAGTGGACATCTTCAGAGCAAAAATTGTTGATATCTCTGAACAGTCACTAACAGTTGAGATTACTGGGGACCCTGGGAAGGTGGTTGCTGTTCAAAGGAACTTAAGCAAGTTTGGGATCAAAGAGCTTGCAAGAACTGGAAAGATTGCTTTGAGACGAGAAAAGATGGGTGCAACAGCTCCTTTCTGGAGATTTTCTGCAGATTCTTATCCAGATCTTGAGAAGTCCACGTCTGATGGTGCTCTTGCAGTGAAAGCAAATCGTACGCTCAATGGTGATGCTGTTACTGCCTCAAGGGGCGACGTTTATCCCGTGGAGCCCTATGATGACTTCCGTTTGAATACAGTTCTTGATGCGCATTGGGGTGTTCTCTATGATGAAGATTCAAGTGGGGTTCGATCACACACCTTGTCGATGATTGTAAATGACTATCCTGGAGTTCTAAACACAGTTACAGGAGTCCTCTCCCGAAGAGGTTATAACATTCAGAGCCTGGCTGTGGGGCCCGCTGAAATGGAGGGGCTTTCTCGCATTACAACAGTTGTTCCCGGTACAGACGATTCAATATCCAAGTTGGTTCAGCAACTCCACAAGTTGATAGATCTTCATGAGGTTCGGGATATTAGTCACTTACCGTTTGCTGAGCGAGAGTTGATGTTGATAAAGATTGCTGTGAACACTACTGCTAGAAGGGATGTTCTTGATATTGCCAGCATTTTCCGTGCCAAAGCTGTTGATGTGTCCGATCATACAATTACCCTTGAGCTGACTGGTGATTTAAATAAGATGGTTGCACTGCAGAAGTTACTAGAGCCCTATGGGATTTGCGAGGTTGCGCGGACTGGGAGGGTGGCGTTGCAGCGGGAGTCTGGTGTGGATTCCACATATCTCCGCGGATACCCCCTTCCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

496

Amino Acids

54.22

Weight (kDa)

8.25

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 75 - 138 5.9e-11 ACT domain
ACT_AHAS_ss PF22629 77 - 142 4.1e-17 AHAS small subunit-like ACT domain
ACT_5 PF13710 85 - 143 2.4e-08 ACT domain
ALS_ss_C PF10369 189 - 245 1.6e-19 Small subunit of acetolactate synthase
ACT PF01842 326 - 391 4.5e-10 ACT domain
ACT_AHAS_ss PF22629 328 - 393 6.9e-22 AHAS small subunit-like ACT domain
ACT_5 PF13710 336 - 396 3.7e-10 ACT domain
ALS_ss_C PF10369 407 - 480 6.5e-28 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
Acc16I TGCGCA 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 115
AccII CGCG 2 cut(s) 1411, 1467
AciI CCGC 8 cut(s) 5, 103, 113, 1080, 1411, 1435, 1465, 1467
AclWI GGATC 2 cut(s) 485, 698
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 2 cut(s) 158, 571
AfaI GTAC 3 cut(s) 499, 838, 1126
AfiI CCNNNNNNNGG 4 cut(s) 119, 508, 652, 1072
AflII CTTAAG 1 cut(s) 676
AgsI TTSAA 6 cut(s) 328, 395, 668, 913, 960, 1137
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 804
AjnI CCWGG 4 cut(s) 287, 646, 1006, 1065
AluBI AGCT 8 cut(s) 90, 136, 145, 400, 700, 752, 1304, 1339
AluI AGCT 8 cut(s) 90, 136, 145, 400, 700, 752, 1304, 1339
Alw21I GWGCWC 1 cut(s) 817
Alw26I GTCTC 2 cut(s) 315, 721
AlwI GGATC 2 cut(s) 485, 698
AlwNI CAGNNNCTG 1 cut(s) 1066
AoxI GGCC 4 cut(s) 39, 100, 110, 1076
ApaI GGGCCC 1 cut(s) 1080
ApeKI GCWGC 1 cut(s) 1432
AspLEI GCGC 2 cut(s) 931, 1411
AspS9I GGNCC 4 cut(s) 40, 643, 1076, 1077
AsuC2I CCSGG 1 cut(s) 1122
AsuHPI GGTGA 5 cut(s) 9, 160, 424, 860, 1358
AsuNHI GCTAGC 1 cut(s) 158
AvaII GGWCC 1 cut(s) 643
BaeGI GKGCMC 1 cut(s) 1080
BanII GRGCYC 3 cut(s) 894, 1080, 1389
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 1 cut(s) 1444
BccI CCATC 3 cut(s) 733, 803, 1354
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 4 cut(s) 289, 648, 1008, 1067
BciVI GTATCC 1 cut(s) 1463
BcnI CCSGG 1 cut(s) 1122
BcoDI GTCTC 2 cut(s) 315, 721
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 4 cut(s) 35, 159, 1260, 1382
BfmI CTRYAG 2 cut(s) 771, 1370
BfrI CTTAAG 1 cut(s) 676
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 1 cut(s) 1463
BglII AGATCT 2 cut(s) 787, 1174
BisI GCNGC 4 cut(s) 6, 103, 113, 1433
BlpI GCTNAGC 1 cut(s) 1215
BlsI GCNGC 4 cut(s) 7, 104, 114, 1434
Bme1390I CCNGG 5 cut(s) 289, 648, 1008, 1067, 1122
Bme18I GGWCC 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 804
BmgT120I GGNCC 4 cut(s) 40, 643, 1076, 1077
BmiI GGNNCC 6 cut(s) 447, 644, 645, 891, 1077, 1078
BmrFI CCNGG 5 cut(s) 289, 648, 1008, 1067, 1122
BmrI ACTGGG 3 cut(s) 53, 648, 1425
BmsI GCATC 3 cut(s) 459, 841, 916
BmtI GCTAGC 1 cut(s) 162
BmuI ACTGGG 3 cut(s) 53, 648, 1425
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 2 cut(s) 781, 1029
Bpu1102I GCTNAGC 1 cut(s) 1215
BpuEI CTTGAG 4 cut(s) 424, 812, 850, 1355
BpuMI CCSGG 1 cut(s) 1122
BsaBI GATNNNNATC 2 cut(s) 473, 780
BsaJI CCNNGG 4 cut(s) 646, 647, 885, 1465
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 508, 652, 1072
Bse1I ACTGG 6 cut(s) 59, 169, 643, 715, 1348, 1420
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 2 cut(s) 473, 780
BseBI CCWGG 4 cut(s) 289, 648, 1008, 1067
BseDI CCNNGG 4 cut(s) 646, 647, 885, 1465
BseGI GGATG 3 cut(s) 253, 517, 1273
BseJI GATNNNNATC 2 cut(s) 473, 780
BseLI CCNNNNNNNGG 4 cut(s) 119, 508, 652, 1072
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 1206
BseNI ACTGG 6 cut(s) 59, 169, 643, 715, 1348, 1420
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 1 cut(s) 1444
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 2 cut(s) 1411, 1467
BshFI GGCC 4 cut(s) 41, 102, 112, 1078
BsiHKAI GWGCWC 1 cut(s) 817
BsiSI CCGG 2 cut(s) 70, 1122
BsiWI CGTACG 1 cut(s) 836
BslFI GGGAC 2 cut(s) 379, 656
BslI CCNNNNNNNGG 4 cut(s) 119, 508, 652, 1072
BsmAI GTCTC 2 cut(s) 315, 721
BsmBI CGTCTC 1 cut(s) 721
BsmFI GGGAC 2 cut(s) 379, 656
BsnI GGCC 4 cut(s) 41, 102, 112, 1078
Bsp120I GGGCCC 1 cut(s) 1076
Bsp1286I GDGCHC 4 cut(s) 817, 894, 1080, 1389
Bsp143I GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
Bsp1720I GCTNAGC 1 cut(s) 1215
BspACI CCGC 8 cut(s) 5, 103, 113, 1080, 1411, 1435, 1465, 1467
BspANI GGCC 4 cut(s) 41, 102, 112, 1078
BspCNI CTCAG 1 cut(s) 1207
BspFNI CGCG 2 cut(s) 1411, 1467
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 6 cut(s) 447, 644, 645, 891, 1077, 1078
BspMAI CTGCAG 2 cut(s) 775, 1374
BspMI ACCTGC 1 cut(s) 376
BspOI GCTAGC 1 cut(s) 162
BspPI GGATC 2 cut(s) 485, 698
BspTI CTTAAG 1 cut(s) 676
BsrBI CCGCTC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 6 cut(s) 59, 169, 643, 715, 1348, 1420
BssECI CCNNGG 4 cut(s) 646, 647, 885, 1465
BssMI GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
Bst2UI CCWGG 4 cut(s) 289, 648, 1008, 1067
Bst4CI ACNGT 6 cut(s) 618, 628, 919, 1024, 1114, 1209
Bst6I CTCTTC 2 cut(s) 119, 1039
BstAFI CTTAAG 1 cut(s) 676
BstC8I GCNNGC 5 cut(s) 160, 541, 702, 1080, 1285
BstDEI CTNAG 2 cut(s) 210, 1215
BstDSI CCRYGG 2 cut(s) 885, 1465
BstF5I GGATG 3 cut(s) 253, 517, 1273
BstFNI CGCG 2 cut(s) 1411, 1467
BstHHI GCGC 2 cut(s) 931, 1411
BstKTI GATC 8 cut(s) 97, 153, 493, 693, 790, 974, 1177, 1321
BstMAI GTCTC 2 cut(s) 315, 721
BstMBI GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 142, 827, 860
BstNI CCWGG 4 cut(s) 289, 648, 1008, 1067
BstSCI CCNGG 5 cut(s) 287, 646, 1006, 1065, 1120
BstSFI CTRYAG 2 cut(s) 771, 1370
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 2 cut(s) 1411, 1467
BstV1I GCAGC 1 cut(s) 1444
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 3 cut(s) 490, 787, 1174
BstYI RGATCY 3 cut(s) 490, 787, 1174
BsuI GTATCC 1 cut(s) 1463
BsuRI GGCC 4 cut(s) 41, 102, 112, 1078
BtgI CCRYGG 2 cut(s) 885, 1465
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 3 cut(s) 253, 517, 1273
BtsI GCAGTG 2 cut(s) 828, 1367
BtsIMutI CAGTG 6 cut(s) 66, 162, 190, 240, 828, 1367
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 5 cut(s) 160, 541, 702, 1080, 1285
CaiI CAGNNNCTG 1 cut(s) 1066
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 2 cut(s) 931, 1411
Cfr13I GGNCC 4 cut(s) 40, 643, 1076, 1077
Cfr42I CCGCGG 1 cut(s) 1468
Csp6I GTAC 3 cut(s) 498, 837, 1125
CviAII CATG 2 cut(s) 74, 1181
CviQI GTAC 3 cut(s) 498, 837, 1125
DdeI CTNAG 2 cut(s) 210, 1215
DpnI GATC 8 cut(s) 96, 152, 492, 692, 789, 973, 1176, 1320
DpnII GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
DraI TTTAAA 1 cut(s) 1353
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 119, 1039
EarI CTCTTC 2 cut(s) 119, 1039
Eco24I GRGCYC 3 cut(s) 894, 1080, 1389
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 643
Eco57I CTGAAG 2 cut(s) 158, 571
EcoO109I RGGNCCY 2 cut(s) 643, 1076
EcoRII CCWGG 4 cut(s) 287, 646, 1006, 1065
EcoRV GATATC 1 cut(s) 607
EcoT38I GRGCYC 3 cut(s) 894, 1080, 1389
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 2 cut(s) 77, 1184
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 2 cut(s) 379, 656
FatI CATG 2 cut(s) 73, 1180
FauI CCCGC 2 cut(s) 1087, 1428
Fnu4HI GCNGC 4 cut(s) 6, 103, 113, 1433
FokI GGATG 3 cut(s) 260, 524, 1280
FriOI GRGCYC 3 cut(s) 894, 1080, 1389
Fsp4HI GCNGC 4 cut(s) 6, 103, 113, 1433
FspAI RTGCGCAY 1 cut(s) 930
FspBI CTAG 4 cut(s) 35, 159, 1260, 1382
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 2 cut(s) 930, 1410
GluI GCNGC 4 cut(s) 6, 103, 113, 1433
GsuI CTGGAG 2 cut(s) 781, 1029
HaeIII GGCC 4 cut(s) 41, 102, 112, 1078
HapII CCGG 2 cut(s) 70, 1122
HhaI GCGC 2 cut(s) 931, 1411
Hin1II CATG 2 cut(s) 77, 1184
Hin6I GCGC 2 cut(s) 929, 1409
HinP1I GCGC 2 cut(s) 929, 1409
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 9 cut(s) 188, 258, 308, 776, 956, 1032, 1133, 1439, 1451
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 2 cut(s) 70, 1122
HphI GGTGA 5 cut(s) 9, 160, 424, 860, 1358
Hpy166II GTNNAC 5 cut(s) 464, 549, 580, 801, 1249
Hpy188I TCNGA 6 cut(s) 133, 590, 613, 808, 1062, 1318
Hpy188III TCNNGA 8 cut(s) 760, 785, 791, 923, 1041, 1181, 1190, 1274
Hpy8I GTNNAC 5 cut(s) 464, 549, 580, 801, 1249
Hpy99I CGWCG 1 cut(s) 878
HpyAV CCTTC 4 cut(s) 207, 646, 1257, 1487
HpyCH4III ACNGT 6 cut(s) 618, 628, 919, 1024, 1114, 1209
HpyCH4IV ACGT 3 cut(s) 460, 803, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 827, 860
HpyF3I CTNAG 2 cut(s) 210, 1215
HpySE526I ACGT 3 cut(s) 460, 803, 876
Hsp92II CATG 2 cut(s) 77, 1184
HspAI GCGC 2 cut(s) 929, 1409
KflI GGGWCCC 1 cut(s) 643
KspAI GTTAAC 1 cut(s) 549
KspI CCGCGG 1 cut(s) 1468
Kzo9I GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
LmnI GCTCC 3 cut(s) 445, 757, 889
Lsp1109I GCAGC 1 cut(s) 1444
LweI GCATC 3 cut(s) 459, 841, 916
MaeI CTAG 4 cut(s) 35, 159, 1260, 1382
MaeII ACGT 3 cut(s) 460, 803, 876
MaeIII GTNAC 6 cut(s) 296, 618, 856, 1024, 1199, 1377
MalI GATC 8 cut(s) 96, 152, 492, 692, 789, 973, 1176, 1320
MbiI CCGCTC 1 cut(s) 115
MboI GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
MboII GAAGA 7 cut(s) 136, 151, 443, 577, 965, 1056, 1169
MflI RGATCY 3 cut(s) 490, 787, 1174
MhlI GDGCHC 4 cut(s) 817, 894, 1080, 1389
MluCI AATT 2 cut(s) 597, 1326
MlyI GAGTC 3 cut(s) 317, 1041, 1448
MseI TTAA 4 cut(s) 483, 548, 677, 1352
MslI CAYNNNNRTG 2 cut(s) 356, 936
MspA1I CMGCKG 4 cut(s) 400, 1082, 1435, 1467
MspCI CTTAAG 1 cut(s) 676
MspI CCGG 2 cut(s) 70, 1122
MspR9I CCNGG 5 cut(s) 289, 648, 1008, 1067, 1122
MvaI CCWGG 4 cut(s) 289, 648, 1008, 1067
MvnI CGCG 2 cut(s) 1411, 1467
MwoI GCNNNNNNNGC 3 cut(s) 142, 827, 860
NciI CCSGG 1 cut(s) 1122
NdeII GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
NheI GCTAGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 77, 1184
NlaIV GGNNCC 6 cut(s) 447, 644, 645, 891, 1077, 1078
NmuCI GTSAC 2 cut(s) 618, 1199
NsbI TGCGCA 1 cut(s) 930
PagI TCATGA 1 cut(s) 1180
PasI CCCWGGG 1 cut(s) 647
PcsI WCGNNNNNNNCGW 1 cut(s) 882
PfeI GAWTC 6 cut(s) 188, 258, 776, 956, 1133, 1451
Pfl23II CGTACG 1 cut(s) 836
PfoI TCCNGGA 1 cut(s) 1006
PkrI GCNGC 4 cut(s) 7, 104, 114, 1434
PleI GAGTC 3 cut(s) 316, 1040, 1447
PpsI GAGTC 3 cut(s) 316, 1040, 1447
PpuMI RGGWCCY 1 cut(s) 643
PsiI TTATAA 1 cut(s) 1053
Psp5II RGGWCCY 1 cut(s) 643
Psp6I CCWGG 4 cut(s) 287, 646, 1006, 1065
PspGI CCWGG 4 cut(s) 287, 646, 1006, 1065
PspLI CGTACG 1 cut(s) 836
PspN4I GGNNCC 6 cut(s) 447, 644, 645, 891, 1077, 1078
PspOMI GGGCCC 1 cut(s) 1076
PspPI GGNCC 4 cut(s) 40, 643, 1076, 1077
PspPPI RGGWCCY 1 cut(s) 643
PstI CTGCAG 2 cut(s) 775, 1374
PstNI CAGNNNCTG 1 cut(s) 1066
PsuI RGATCY 3 cut(s) 490, 787, 1174
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 3 cut(s) 499, 838, 1126
RsaNI GTAC 3 cut(s) 498, 837, 1125
RseI CAYNNNNRTG 2 cut(s) 356, 936
SacII CCGCGG 1 cut(s) 1468
SaqAI TTAA 4 cut(s) 483, 548, 677, 1352
SatI GCNGC 4 cut(s) 6, 103, 113, 1433
Sau3AI GATC 8 cut(s) 94, 150, 490, 690, 787, 971, 1174, 1318
Sau96I GGNCC 4 cut(s) 40, 643, 1076, 1077
SchI GAGTC 3 cut(s) 317, 1041, 1448
ScrFI CCNGG 5 cut(s) 289, 648, 1008, 1067, 1122
SduI GDGCHC 4 cut(s) 817, 894, 1080, 1389
SfaNI GCATC 3 cut(s) 459, 841, 916
SfcI CTRYAG 2 cut(s) 771, 1370
Sfr303I CCGCGG 1 cut(s) 1468
SgrBI CCGCGG 1 cut(s) 1468
SinI GGWCC 1 cut(s) 643
SmiI ATTTAAAT 1 cut(s) 1353
SmiMI CAYNNNNRTG 2 cut(s) 356, 936
SmlI CTYRAG 5 cut(s) 439, 676, 791, 865, 1334
SmoI CTYRAG 5 cut(s) 439, 676, 791, 865, 1334
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 2 cut(s) 597, 1326
SsiI CCGC 8 cut(s) 5, 103, 113, 1080, 1411, 1435, 1465, 1467
SspMI CTAG 4 cut(s) 35, 159, 1260, 1382
StyD4I CCNGG 5 cut(s) 287, 646, 1006, 1065, 1120
SwaI ATTTAAAT 1 cut(s) 1353
TaaI ACNGT 6 cut(s) 618, 628, 919, 1024, 1114, 1209
TaiI ACGT 3 cut(s) 463, 806, 879
TaqI TCGA 5 cut(s) 93, 306, 429, 970, 986
TasI AATT 2 cut(s) 597, 1326
TauI GCSGC 3 cut(s) 8, 105, 115
TfiI GAWTC 6 cut(s) 188, 258, 776, 956, 1133, 1451
Tru1I TTAA 4 cut(s) 483, 548, 677, 1352
Tru9I TTAA 4 cut(s) 483, 548, 677, 1352
TscAI CASTG 6 cut(s) 66, 169, 190, 240, 828, 1374
TseFI GTSAC 2 cut(s) 618, 1199
TseI GCWGC 1 cut(s) 1432
Tsp45I GTSAC 2 cut(s) 618, 1199
TspDTI ATGAA 3 cut(s) 189, 966, 1169
TspGWI ACGGA 3 cut(s) 896, 1283, 1470
TspRI CASTG 6 cut(s) 66, 169, 190, 240, 828, 1374
Vha464I CTTAAG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 4 cut(s) 35, 159, 1260, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.