Rh5CG099700

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008290
Physical Location & Seq
Reverse (-)
7963845 .. 7969916
6072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG099700.1

Sequence Viewer

Length: 1491 bp
ATGGCGGCTTCTTTGTCATCACCACCACCGTGCTCAACTCCACTGATTACCCAGAAACCATGTTTCAGACTCGATTTGGGACGTGGGTTCTTGAAAAGTTTCGACCTTGGCCATCAGAGGCCACCAAAGTTGAGGTCTTTGGAGTCGTCTAAGGAGCTGGTGGTCTCTGCTAGCAGCAACAATGCTGTGTCTGTCACCGACCCTGCGCCTTCATTGACTCGCTCTAAGGTGAAACGCCATACGATTTCAGTGTTCGTTGGTGATGAGAGTGGAATTATAAACCGAATAGCAGGGGTTTTTGCCAGGAGGGGTTACAATATAGAGTCCCTTGCTGTTGGCTTGAACAAGGACAGGGCTCTCTTTACCATAGTTGTCTCAGGCACTGAAAATGTTTTGCGGCAAGTTGTTGAACAGCTCAACAAGTTGGTGAATGTGATAAAGGTTGAAGATATCTCAAGGGAGCCACAAGTAGAACGTGAATTGATGCTTATCAAACTTAATGCAGATCCAAGCAACCGTGCAGAGATCATATGGTTAGTGGACATCTTCAGAGCGAAAATTGTGGACATCTCTGAACATTCACTGACAATTGAGGTTACTGGTGACCCTGGCAAAATGGTTGCTGTTCAAAGAAACTTGAGCAAATTTGGAATCCAAGAACTTGCAAGAACTGGAAAGATTGCTCTGAGACGAGAAAAGATGGGTGAGACAGCTCCTTTTTGGAGATTTTCTGCAGCTTCTTATCCTGATCTTGAAAGGACAATGTCTGATGATGAAGTTGCAGTGAAACCAAATCATTCAGCCAATGGTGCTGCTAGTATTGCCTCACAGGGTGATGTTTATCCTGTGGAGCCTTATGATGACTTTCCTTCAAATCAAGTACTAGATGCTCATTGGGGTGTTCTCTATGATGAAGATTCAAGTGGGGTTCAATCACACACTTTGTGTATGCTTGTGAATGATGTACCTGGAGTCCTGAACATAGTTACAGGAGTCATATCTCGAAGAGGTTATAACATTCAGAGTCTAGCTGTGGGGCCTTCTGAGAAGGAAGGGCTTTCTCGCATTACAACTGTTGTTCCTGGTACTGATGACTCAATTACCAAGCTGGTCCAGCAACTTCGCAAGTTAATAGATCTTCATGAGGTTCGGGATATTACACACTTACCATTTGCTGAGCGAGAGTTGATGTTAATAAAGATTGCTGTAAACACTACTGCTAGAAGGGATGTTCTCGATATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACCATTACTCTTGAGCTTACTGGCGATTTGAATAAAATGGTTGCACTGCAGAAGTTACTAGAGCCCTATGGGCTTTGCGAGGTTTGTCTTTTCATTATTTGTTGTTGTAACGAATGGCTAATTGAAACTGCTGTTAAACGCTCATGCTTTTGTTGCATGGTCTTGTTTCTGGGTTTCCACATGGTGTTAAATGATAGTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

496

Amino Acids

54.78

Weight (kDa)

6.11

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 80 - 143 4.4e-11 ACT domain
ACT_AHAS_ss PF22629 82 - 147 9.2e-17 AHAS small subunit-like ACT domain
ACT_5 PF13710 90 - 148 5.3e-08 ACT domain
ALS_ss_C PF10369 159 - 232 2.5e-28 Small subunit of acetolactate synthase
ACT_AHAS_ss PF22629 316 - 380 1e-21 AHAS small subunit-like ACT domain
ACT PF01842 318 - 378 5.5e-10 ACT domain
ACT_5 PF13710 322 - 383 9.7e-11 ACT domain
ALS_ss_C PF10369 394 - 456 1.1e-19 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 278, 1014
AciI CCGC 2 cut(s) 5, 397
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 532
AdeI CACNNNGTG 3 cut(s) 833, 945, 1468
AfaI GTAC 3 cut(s) 882, 966, 1087
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 4 cut(s) 302, 607, 967, 1081
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 7 cut(s) 157, 415, 713, 737, 1031, 1108, 1300
AluI AGCT 7 cut(s) 157, 415, 713, 737, 1031, 1108, 1300
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 4 cut(s) 169, 379, 682, 701
AlwI GGATC 1 cut(s) 500
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 3 cut(s) 109, 119, 1037
ApeKI GCWGC 3 cut(s) 174, 734, 812
ApoI RAATTY 1 cut(s) 644
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 1037, 1111
AsuHPI GGTGA 8 cut(s) 12, 187, 241, 272, 439, 614, 716, 845
AsuNHI GCTAGC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 1111
BalI TGGCCA 1 cut(s) 111
BanII GRGCYC 2 cut(s) 358, 1350
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 186, 746, 799
BccI CCATC 2 cut(s) 120, 694
BceAI ACGGC 1 cut(s) 1250
BciT130I CCWGG 4 cut(s) 304, 609, 969, 1083
BclI TGATCA 1 cut(s) 1279
BcoDI GTCTC 4 cut(s) 169, 379, 682, 701
BfaI CTAG 6 cut(s) 171, 816, 884, 1028, 1221, 1343
BfmI CTRYAG 2 cut(s) 732, 1331
BglI GCCNNNNNGGC 1 cut(s) 1354
BglII AGATCT 1 cut(s) 1135
BisI GCNGC 5 cut(s) 6, 175, 398, 735, 813
BlpI GCTNAGC 1 cut(s) 1176
BlsI GCNGC 5 cut(s) 7, 176, 399, 736, 814
BmcAI AGTACT 1 cut(s) 882
Bme1390I CCNGG 4 cut(s) 304, 609, 969, 1083
Bme18I GGWCC 1 cut(s) 1111
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 1037, 1111
BmiI GGNNCC 3 cut(s) 462, 852, 1038
BmrFI CCNGG 4 cut(s) 304, 609, 969, 1083
BmsI GCATC 2 cut(s) 474, 877
BmtI GCTAGC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 990
Bpu1102I GCTNAGC 1 cut(s) 1176
BpuEI CTTGAG 3 cut(s) 439, 658, 1316
BsaBI GATNNNNATC 2 cut(s) 488, 840
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 2 cut(s) 106, 607
Bse1I ACTGG 3 cut(s) 604, 676, 1309
Bse8I GATNNNNATC 2 cut(s) 488, 840
BseBI CCWGG 4 cut(s) 304, 609, 969, 1083
BseDI CCNNGG 2 cut(s) 106, 607
BseGI GGATG 1 cut(s) 1234
BseJI GATNNNNATC 2 cut(s) 488, 840
BseMII CTCAG 4 cut(s) 390, 677, 1035, 1167
BseNI ACTGG 3 cut(s) 604, 676, 1309
BseXI GCAGC 3 cut(s) 186, 746, 799
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 3 cut(s) 111, 121, 1039
BsiHKAI GWGCWC 1 cut(s) 35
BslFI GGGAC 2 cut(s) 93, 310
BsmAI GTCTC 4 cut(s) 169, 379, 682, 701
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 2 cut(s) 93, 310
BsnI GGCC 3 cut(s) 111, 121, 1039
Bso31I GGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 3 cut(s) 35, 358, 1350
Bsp143I GATC 5 cut(s) 505, 525, 748, 1135, 1279
Bsp1720I GCTNAGC 1 cut(s) 1176
BspACI CCGC 2 cut(s) 5, 397
BspANI GGCC 3 cut(s) 111, 121, 1039
BspCNI CTCAG 4 cut(s) 389, 678, 1036, 1168
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 3 cut(s) 462, 852, 1038
BspMAI CTGCAG 2 cut(s) 736, 1335
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 169
BsrI ACTGG 3 cut(s) 604, 676, 1309
BssECI CCNNGG 2 cut(s) 106, 607
BssMI GATC 5 cut(s) 505, 525, 748, 1135, 1279
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 4 cut(s) 304, 609, 969, 1083
Bst4CI ACNGT 3 cut(s) 30, 518, 1075
Bst6I CTCTTC 1 cut(s) 1000
BstC8I GCNNGC 2 cut(s) 172, 1246
BstDEI CTNAG 6 cut(s) 150, 225, 376, 686, 1044, 1176
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 1 cut(s) 1234
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 5 cut(s) 508, 528, 751, 1138, 1282
BstMAI GTCTC 4 cut(s) 169, 379, 682, 701
BstMBI GATC 5 cut(s) 505, 525, 748, 1135, 1279
BstMWI GCNNNNNNNGC 5 cut(s) 809, 821, 1114, 1354, 1437
BstNI CCWGG 4 cut(s) 304, 609, 969, 1083
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 4 cut(s) 302, 607, 967, 1081
BstSFI CTRYAG 2 cut(s) 732, 1331
BstV1I GCAGC 3 cut(s) 186, 746, 799
BstX2I RGATCY 2 cut(s) 505, 1135
BstYI RGATCY 2 cut(s) 505, 1135
BsuRI GGCC 3 cut(s) 111, 121, 1039
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 1234
BtsI GCAGTG 2 cut(s) 789, 1328
BtsIMutI CAGTG 6 cut(s) 41, 255, 381, 581, 789, 1328
Cac8I GCNNGC 2 cut(s) 172, 1246
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 1037, 1111
Csp6I GTAC 3 cut(s) 881, 965, 1086
CspCI CAANNNNNGTGG 2 cut(s) 111, 146
CviAII CATG 5 cut(s) 60, 1142, 1428, 1441, 1465
CviQI GTAC 3 cut(s) 881, 965, 1086
DdeI CTNAG 6 cut(s) 150, 225, 376, 686, 1044, 1176
DpnI GATC 5 cut(s) 507, 527, 750, 1137, 1281
DpnII GATC 5 cut(s) 505, 525, 748, 1135, 1279
DraIII CACNNNGTG 3 cut(s) 833, 945, 1468
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 1000
EarI CTCTTC 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 358, 1350
Eco31I GGTCTC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 1111
Eco57I CTGAAG 1 cut(s) 532
Eco91I GGTNACC 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 1037
EcoO65I GGTNACC 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 302, 607, 967, 1081
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 2 cut(s) 358, 1350
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 5 cut(s) 63, 1145, 1431, 1444, 1468
FaqI GGGAC 2 cut(s) 93, 310
FatI CATG 5 cut(s) 59, 1141, 1427, 1440, 1464
FauNDI CATATG 1 cut(s) 530
FbaI TGATCA 1 cut(s) 1279
Fnu4HI GCNGC 5 cut(s) 6, 175, 398, 735, 813
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 2 cut(s) 358, 1350
Fsp4HI GCNGC 5 cut(s) 6, 175, 398, 735, 813
FspBI CTAG 6 cut(s) 171, 816, 884, 1028, 1221, 1343
GlaI GCGC 1 cut(s) 207
GluI GCNGC 5 cut(s) 6, 175, 398, 735, 813
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 3 cut(s) 111, 121, 1039
HhaI GCGC 1 cut(s) 208
Hin1II CATG 5 cut(s) 63, 1145, 1431, 1444, 1468
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HphI GGTGA 8 cut(s) 12, 187, 241, 272, 439, 614, 716, 845
Hpy166II GTNNAC 3 cut(s) 541, 565, 1210
Hpy188I TCNGA 9 cut(s) 68, 117, 551, 574, 687, 769, 1023, 1045, 1279
Hpy188III TCNNGA 9 cut(s) 91, 746, 752, 976, 1002, 1142, 1151, 1235, 1295
Hpy8I GTNNAC 3 cut(s) 541, 565, 1210
HpyAV CCTTC 6 cut(s) 219, 879, 1042, 1046, 1050, 1218
HpyCH4III ACNGT 3 cut(s) 30, 518, 1075
HpyCH4IV ACGT 2 cut(s) 82, 475
HpyCH4V TGCA 8 cut(s) 503, 521, 665, 734, 782, 1328, 1333, 1440
HpyF10VI GCNNNNNNNGC 5 cut(s) 809, 821, 1114, 1354, 1437
HpyF3I CTNAG 6 cut(s) 150, 225, 376, 686, 1044, 1176
HpySE526I ACGT 2 cut(s) 82, 475
Hsp92II CATG 5 cut(s) 63, 1145, 1431, 1444, 1468
HspAI GCGC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 1279
Kzo9I GATC 5 cut(s) 505, 525, 748, 1135, 1279
LmnI GCTCC 4 cut(s) 154, 460, 718, 850
Lsp1109I GCAGC 3 cut(s) 186, 746, 799
LweI GCATC 2 cut(s) 474, 877
MaeI CTAG 6 cut(s) 171, 816, 884, 1028, 1221, 1343
MaeII ACGT 2 cut(s) 82, 475
MaeIII GTNAC 7 cut(s) 193, 311, 595, 602, 985, 1338, 1391
MalI GATC 5 cut(s) 507, 527, 750, 1137, 1281
MboI GATC 5 cut(s) 505, 525, 748, 1135, 1279
MboII GAAGA 5 cut(s) 458, 538, 926, 1017, 1130
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 505, 1135
MhlI GDGCHC 3 cut(s) 35, 358, 1350
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 8 cut(s) 273, 479, 558, 588, 644, 1098, 1404, 1486
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 8 cut(s) 63, 152, 211, 332, 981, 1002, 1033, 1088
MnlI CCTC 8 cut(s) 111, 126, 300, 586, 835, 1001, 1138, 1357
Mox20I TGGCCA 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 111
MseI TTAA 6 cut(s) 498, 1130, 1193, 1419, 1472, 1489
MslI CAYNNNNRTG 2 cut(s) 28, 897
Msp20I TGGCCA 1 cut(s) 111
MspR9I CCNGG 4 cut(s) 304, 609, 969, 1083
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 4 cut(s) 304, 609, 969, 1083
MwoI GCNNNNNNNGC 5 cut(s) 809, 821, 1114, 1354, 1437
NdeI CATATG 1 cut(s) 530
NdeII GATC 5 cut(s) 505, 525, 748, 1135, 1279
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 5 cut(s) 63, 1145, 1431, 1444, 1468
NlaIV GGNNCC 3 cut(s) 462, 852, 1038
NmuCI GTSAC 2 cut(s) 193, 602
OliI CACNNNNGTG 1 cut(s) 28
PagI TCATGA 1 cut(s) 1141
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 651, 917
PflFI GACNNNGTC 1 cut(s) 763
PkrI GCNGC 5 cut(s) 7, 176, 399, 736, 814
PleI GAGTC 8 cut(s) 63, 151, 211, 331, 980, 1001, 1032, 1088
PpsI GAGTC 8 cut(s) 63, 151, 211, 331, 980, 1001, 1032, 1088
PsiI TTATAA 2 cut(s) 278, 1014
Psp6I CCWGG 4 cut(s) 302, 607, 967, 1081
PspEI GGTNACC 1 cut(s) 602
PspGI CCWGG 4 cut(s) 302, 607, 967, 1081
PspN4I GGNNCC 3 cut(s) 462, 852, 1038
PspPI GGNCC 2 cut(s) 1037, 1111
PstI CTGCAG 2 cut(s) 736, 1335
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 2 cut(s) 505, 1135
PsyI GACNNNGTC 1 cut(s) 763
RsaI GTAC 3 cut(s) 882, 966, 1087
RsaNI GTAC 3 cut(s) 881, 965, 1086
RseI CAYNNNNRTG 2 cut(s) 28, 897
SaqAI TTAA 6 cut(s) 498, 1130, 1193, 1419, 1472, 1489
SatI GCNGC 5 cut(s) 6, 175, 398, 735, 813
Sau3AI GATC 5 cut(s) 505, 525, 748, 1135, 1279
Sau96I GGNCC 2 cut(s) 1037, 1111
ScaI AGTACT 1 cut(s) 882
SchI GAGTC 8 cut(s) 63, 152, 211, 332, 981, 1002, 1033, 1088
ScrFI CCNGG 4 cut(s) 304, 609, 969, 1083
SduI GDGCHC 3 cut(s) 35, 358, 1350
SfaNI GCATC 2 cut(s) 474, 877
SfcI CTRYAG 2 cut(s) 732, 1331
SinI GGWCC 1 cut(s) 1111
SmiMI CAYNNNNRTG 2 cut(s) 28, 897
SmlI CTYRAG 3 cut(s) 454, 637, 1295
SmoI CTYRAG 3 cut(s) 454, 637, 1295
Sse9I AATT 8 cut(s) 273, 479, 558, 588, 644, 1098, 1404, 1486
SsiI CCGC 2 cut(s) 5, 397
SspMI CTAG 6 cut(s) 171, 816, 884, 1028, 1221, 1343
StyD4I CCNGG 4 cut(s) 302, 607, 967, 1081
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 30, 518, 1075
TaiI ACGT 2 cut(s) 85, 478
TaqI TCGA 4 cut(s) 72, 102, 1003, 1236
TasI AATT 8 cut(s) 273, 479, 558, 588, 644, 1098, 1404, 1486
TatI WGTACW 1 cut(s) 880
TauI GCSGC 2 cut(s) 8, 400
TfiI GAWTC 2 cut(s) 651, 917
Tru1I TTAA 6 cut(s) 498, 1130, 1193, 1419, 1472, 1489
Tru9I TTAA 6 cut(s) 498, 1130, 1193, 1419, 1472, 1489
TscAI CASTG 6 cut(s) 48, 255, 388, 588, 789, 1335
TseFI GTSAC 2 cut(s) 193, 602
TseI GCWGC 3 cut(s) 174, 734, 812
Tsp45I GTSAC 2 cut(s) 193, 602
TspDTI ATGAA 5 cut(s) 201, 789, 927, 1130, 1366
TspGWI ACGGA 1 cut(s) 1244
TspRI CASTG 6 cut(s) 48, 255, 388, 588, 789, 1335
Tth111I GACNNNGTC 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 1111
XapI RAATTY 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 6 cut(s) 171, 816, 884, 1028, 1221, 1343
ZrmI AGTACT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.