Rroxscaffold_1G00064800

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008290
Physical Location & Seq
Forward (+)
86600280 .. 86605623
5344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064800.1

Sequence Viewer

Length: 1638 bp
ATGGCGGCTTCTTTGTCATCACCACCACCGTGCTCAACTCCACTGATTACCCATAAACCATGTTTCAGACTCGATTTGGGACGTGGGTTTATGAAAAGTTTCGACCTTGGCCATCAGAGGGCACCAAAGTTGAGGTCTTTGGAGTCGTCTAAGGAGCTGGTGGTCTCTGCTAGCAGCAACAATGCTGTGTCTGTCACTGACCCTGCGCCTTCATTGGCTCGCTCTACTTTTCTGGGTGAAATTGGGTTCCTTTATGTCTTGGGAGTTTTGGGTTCAGCTGAGTTTAGTGGGTTTTGTTTCATCAGTTACCTGATTGAGATAAGTAAGTCTCAGGTTATAGACCTGAACTGTAGTTGGGTGAAACGCCATACGATTTCAGTGTTCGTTGGTGATGAGAGTGGAATTATAAACCGAATAGCAGGGGTGTTTGCCAGGAGGGGTTACAATATTGAGTCCCTTGCTGTTGGCTTGAACAAGGACAGGGCTCTCTTTACCATAGTTGTCTCAGGCACTGAAAATGTTTTGCGGCAAGTTGTTGAACAGCTCAACAAGTTGGTGAATGTGATAAAGGTTGAAGATATCTCAAGGGAGCCACAAGTAGAACGTGAATTGATGCTTATCAAACTTAATGCAGATCCAAGCAACCGTGCAGAGATCATATGGTTAGTGGACATCTTCAGAGCAAAAATTGTGGACATCTCTGAACATTCACTGACAATTGAGGTTACTGGTGACCCTGGGAAAATGGTTGCTGTTCAAAGAAACTTGAGTAAAATTTGGAATCAAAGAACTTGCAAGAACTGGAAAGGCAAGTTTTATATTTGCATTGGCATGTTTATCATTTATCTATGTGTGTATGTATCTGTTAATACTATTGCTCTGAGACGAGAAAAGATGGGTGAGACAGCTCCTTTTTGGAGATTTTCTGCAGCGTCTTATCCTGATCTTGAAGGGACAATGTCTGATGATGAAGTTGCAGTGAAACCAAAACATTCAGCCAATGGTGCTGCTAGTATTGCCTCACAGGGTGATGTTTATCCTGTGGAGCCCTATGATGACTTTCCTTCAAATCAAGTACTAGATGCTCATTGGGGTGTTCTCTATGATGAAGATTCAAGTGGTGTTCAATCACACACTTTGTGTATGCTTGTGAATGACGTACCTGGAGTCCTGAACATAGTTACAGGAGTCATATCTCGAAGAGGTTATAACATTCAGCTGTGGGGCCTTCTGAGAGAGGAAGGGCTTTCTCGCATTACAACTGTTGTTCCTGGTACTGATGACTCAATTACCAAGCTGGTCCAGCAACTTCGCAAGTTAATAGATCTTCATGAGGTTCGGGATATTACACACTTACCATTTGCTGAGCGAGAGTTGATGTTAATAAAGATTGCTGTCAACACTACTGCTAGAAGGGATGTTCTTGATATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACAATTACTCTTGAGCTTACTGGCGATTTGAATAAAATGGTTGCACTGCAGAAGTTACTAGAGCCCTATGGGCTTTGCGAGATTGCACGGACTGGGAGAGTGGCATTGGAGCGGGGCTCTGGTGTGGATTCCACTTCTCTCCGTGGCTATCCCCTTCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

545

Amino Acids

60.25

Weight (kDa)

6.4

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 123 - 186 5e-11 ACT domain
ACT_AHAS_ss PF22629 125 - 190 1e-16 AHAS small subunit-like ACT domain
ACT_5 PF13710 133 - 191 6e-08 ACT domain
ALS_ss_C PF10369 202 - 258 1.9e-18 Small subunit of acetolactate synthase
ACT_AHAS_ss PF22629 381 - 443 2.1e-16 AHAS small subunit-like ACT domain
ACT_5 PF13710 387 - 446 9.6e-07 ACT domain
ALS_ss_C PF10369 457 - 530 3.7e-27 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 407, 1209, 1636
AccB1I GGYRCC 1 cut(s) 121
AccBSI CCGCTC 1 cut(s) 1585
AciI CCGC 3 cut(s) 5, 526, 1585
AclWI GGATC 1 cut(s) 629
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 774
AcuI CTGAAG 1 cut(s) 661
AdeI CACNNNGTG 2 cut(s) 1028, 1140
AfaI GTAC 3 cut(s) 1077, 1161, 1276
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 9 cut(s) 472, 539, 575, 758, 950, 1068, 1116, 1127, 1504
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 4 cut(s) 431, 736, 1162, 1270
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 7 cut(s) 157, 278, 544, 908, 1219, 1297, 1489
AluI AGCT 7 cut(s) 157, 278, 544, 908, 1219, 1297, 1489
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 5 cut(s) 169, 333, 508, 877, 896
AlwI GGATC 1 cut(s) 629
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 2 cut(s) 109, 1225
ApeKI GCWGC 3 cut(s) 174, 929, 1007
ApoI RAATTY 1 cut(s) 774
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 1225, 1300
AsuHPI GGTGA 8 cut(s) 12, 248, 370, 401, 568, 743, 911, 1040
AsuNHI GCTAGC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 1300
BaeGI GKGCMC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 4 cut(s) 487, 1050, 1539, 1592
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 186, 941, 994
BccI CCATC 2 cut(s) 120, 889
BceAI ACGGC 1 cut(s) 1439
BciT130I CCWGG 4 cut(s) 433, 738, 1164, 1272
BclI TGATCA 1 cut(s) 1468
BcoDI GTCTC 5 cut(s) 169, 333, 508, 877, 896
BfaI CTAG 5 cut(s) 171, 1011, 1079, 1410, 1532
BfmI CTRYAG 3 cut(s) 349, 927, 1520
BglI GCCNNNNNGGC 1 cut(s) 1543
BglII AGATCT 1 cut(s) 1324
BisI GCNGC 5 cut(s) 6, 175, 527, 930, 1008
BlpI GCTNAGC 1 cut(s) 1365
BlsI GCNGC 5 cut(s) 7, 176, 528, 931, 1009
BmcAI AGTACT 1 cut(s) 1077
Bme1390I CCNGG 4 cut(s) 433, 738, 1164, 1272
Bme18I GGWCC 1 cut(s) 1300
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 1225, 1300
BmiI GGNNCC 5 cut(s) 123, 248, 591, 1047, 1226
BmrFI CCNGG 4 cut(s) 433, 738, 1164, 1272
BmrI ACTGGG 1 cut(s) 1575
BmsI GCATC 2 cut(s) 603, 1072
BmtI GCTAGC 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 1575
BplI GAGNNNNNCTC 2 cut(s) 1574, 1606
BpmI CTGGAG 1 cut(s) 1185
Bpu1102I GCTNAGC 1 cut(s) 1365
BpuEI CTTGAG 3 cut(s) 568, 787, 1505
BsaBI GATNNNNATC 2 cut(s) 617, 1035
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 4 cut(s) 106, 736, 737, 1615
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 4 cut(s) 733, 806, 1498, 1570
Bse8I GATNNNNATC 2 cut(s) 617, 1035
BseBI CCWGG 4 cut(s) 433, 738, 1164, 1272
BseDI CCNNGG 4 cut(s) 106, 736, 737, 1615
BseGI GGATG 1 cut(s) 1423
BseJI GATNNNNATC 2 cut(s) 617, 1035
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 6 cut(s) 270, 344, 519, 872, 1223, 1356
BseNI ACTGG 4 cut(s) 733, 806, 1498, 1570
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 3 cut(s) 186, 941, 994
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 2 cut(s) 111, 1227
BshNI GGYRCC 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 35
BslFI GGGAC 3 cut(s) 93, 439, 967
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 5 cut(s) 169, 333, 508, 877, 896
BsmBI CGTCTC 1 cut(s) 877
BsmFI GGGAC 3 cut(s) 93, 439, 967
BsnI GGCC 2 cut(s) 111, 1227
Bso31I GGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 6 cut(s) 35, 124, 487, 1050, 1539, 1592
Bsp143I GATC 5 cut(s) 634, 654, 943, 1324, 1468
Bsp1720I GCTNAGC 1 cut(s) 1365
BspACI CCGC 3 cut(s) 5, 526, 1585
BspANI GGCC 2 cut(s) 111, 1227
BspCNI CTCAG 6 cut(s) 271, 343, 518, 873, 1224, 1357
BspHI TCATGA 1 cut(s) 1330
BspLI GGNNCC 5 cut(s) 123, 248, 591, 1047, 1226
BspMAI CTGCAG 2 cut(s) 931, 1524
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 1585
BsrI ACTGG 4 cut(s) 733, 806, 1498, 1570
BssECI CCNNGG 4 cut(s) 106, 736, 737, 1615
BssMI GATC 5 cut(s) 634, 654, 943, 1324, 1468
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 4 cut(s) 433, 738, 1164, 1272
Bst4CI ACNGT 4 cut(s) 30, 350, 647, 1264
Bst6I CTCTTC 1 cut(s) 1195
BstC8I GCNNGC 3 cut(s) 172, 220, 1435
BstDEI CTNAG 7 cut(s) 150, 279, 330, 505, 881, 1232, 1365
BstDSI CCRYGG 1 cut(s) 1615
BstEII GGTNACC 1 cut(s) 731
BstF5I GGATG 1 cut(s) 1423
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 5 cut(s) 637, 657, 946, 1327, 1471
BstMAI GTCTC 5 cut(s) 169, 333, 508, 877, 896
BstMBI GATC 5 cut(s) 634, 654, 943, 1324, 1468
BstMWI GCNNNNNNNGC 4 cut(s) 1004, 1016, 1303, 1543
BstNI CCWGG 4 cut(s) 433, 738, 1164, 1272
BstNSI RCATGY 1 cut(s) 835
BstPI GGTNACC 1 cut(s) 731
BstSCI CCNGG 4 cut(s) 431, 736, 1162, 1270
BstSFI CTRYAG 3 cut(s) 349, 927, 1520
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 3 cut(s) 186, 941, 994
BstX2I RGATCY 2 cut(s) 634, 1324
BstYI RGATCY 2 cut(s) 634, 1324
BsuRI GGCC 2 cut(s) 111, 1227
BtgI CCRYGG 1 cut(s) 1615
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 1423
BtsI GCAGTG 2 cut(s) 984, 1517
BtsIMutI CAGTG 7 cut(s) 41, 195, 384, 510, 710, 984, 1517
Cac8I GCNNGC 3 cut(s) 172, 220, 1435
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 1 cut(s) 1330
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 1225, 1300
CseI GACGC 1 cut(s) 921
Csp6I GTAC 3 cut(s) 1076, 1160, 1275
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 3 cut(s) 60, 832, 1331
CviQI GTAC 3 cut(s) 1076, 1160, 1275
DdeI CTNAG 7 cut(s) 150, 279, 330, 505, 881, 1232, 1365
DpnI GATC 5 cut(s) 636, 656, 945, 1326, 1470
DpnII GATC 5 cut(s) 634, 654, 943, 1324, 1468
DraIII CACNNNGTG 2 cut(s) 1028, 1140
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 1195
EarI CTCTTC 1 cut(s) 1195
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 4 cut(s) 487, 1050, 1539, 1592
Eco31I GGTCTC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 1300
Eco57I CTGAAG 1 cut(s) 661
Eco91I GGTNACC 1 cut(s) 731
EcoO109I RGGNCCY 1 cut(s) 1225
EcoO65I GGTNACC 1 cut(s) 731
EcoRII CCWGG 4 cut(s) 431, 736, 1162, 1270
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 4 cut(s) 487, 1050, 1539, 1592
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 877
FaeI CATG 3 cut(s) 63, 835, 1334
FaqI GGGAC 3 cut(s) 93, 439, 967
FatI CATG 3 cut(s) 59, 831, 1330
FauI CCCGC 1 cut(s) 1578
FauNDI CATATG 1 cut(s) 659
FbaI TGATCA 1 cut(s) 1468
Fnu4HI GCNGC 5 cut(s) 6, 175, 527, 930, 1008
FokI GGATG 1 cut(s) 1430
FriOI GRGCYC 4 cut(s) 487, 1050, 1539, 1592
Fsp4HI GCNGC 5 cut(s) 6, 175, 527, 930, 1008
FspBI CTAG 5 cut(s) 171, 1011, 1079, 1410, 1532
GlaI GCGC 1 cut(s) 207
GluI GCNGC 5 cut(s) 6, 175, 527, 930, 1008
GsuI CTGGAG 1 cut(s) 1185
HaeIII GGCC 2 cut(s) 111, 1227
HgaI GACGC 1 cut(s) 921
HhaI GCGC 1 cut(s) 208
Hin1II CATG 3 cut(s) 63, 835, 1334
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 1399
HindII GTYRAC 1 cut(s) 1399
HinfI GANTC 9 cut(s) 69, 143, 452, 781, 1112, 1167, 1188, 1283, 1601
HphI GGTGA 8 cut(s) 12, 248, 370, 401, 568, 743, 911, 1040
Hpy166II GTNNAC 3 cut(s) 670, 694, 1399
Hpy188I TCNGA 8 cut(s) 68, 117, 680, 703, 882, 964, 1233, 1468
Hpy188III TCNNGA 8 cut(s) 941, 947, 1171, 1197, 1331, 1340, 1424, 1484
Hpy8I GTNNAC 3 cut(s) 670, 694, 1399
HpyAV CCTTC 7 cut(s) 219, 944, 1074, 1235, 1238, 1407, 1637
HpyCH4III ACNGT 4 cut(s) 30, 350, 647, 1264
HpyCH4IV ACGT 3 cut(s) 82, 604, 1158
HpyCH4V TGCA 9 cut(s) 632, 650, 795, 825, 929, 977, 1517, 1522, 1559
HpyF10VI GCNNNNNNNGC 4 cut(s) 1004, 1016, 1303, 1543
HpyF3I CTNAG 7 cut(s) 150, 279, 330, 505, 881, 1232, 1365
HpySE526I ACGT 3 cut(s) 82, 604, 1158
Hsp92II CATG 3 cut(s) 63, 835, 1334
HspAI GCGC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 1468
Kzo9I GATC 5 cut(s) 634, 654, 943, 1324, 1468
LmnI GCTCC 5 cut(s) 154, 589, 913, 1045, 1582
Lsp1109I GCAGC 3 cut(s) 186, 941, 994
LweI GCATC 2 cut(s) 603, 1072
MaeI CTAG 5 cut(s) 171, 1011, 1079, 1410, 1532
MaeII ACGT 3 cut(s) 82, 604, 1158
MaeIII GTNAC 7 cut(s) 193, 305, 440, 724, 731, 1180, 1527
MalI GATC 5 cut(s) 636, 656, 945, 1326, 1470
MbiI CCGCTC 1 cut(s) 1585
MboI GATC 5 cut(s) 634, 654, 943, 1324, 1468
MboII GAAGA 5 cut(s) 587, 667, 1121, 1212, 1319
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 634, 1324
MhlI GDGCHC 6 cut(s) 35, 124, 487, 1050, 1539, 1592
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 8 cut(s) 240, 402, 608, 687, 717, 774, 1287, 1476
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 6 cut(s) 63, 152, 461, 1176, 1197, 1277
MnlI CCTC 8 cut(s) 111, 126, 429, 715, 1030, 1196, 1231, 1327
Mox20I TGGCCA 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 111
MseI TTAA 4 cut(s) 627, 867, 1319, 1382
MslI CAYNNNNRTG 3 cut(s) 28, 830, 1092
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 278, 1219
MspR9I CCNGG 4 cut(s) 433, 738, 1164, 1272
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 4 cut(s) 433, 738, 1164, 1272
MwoI GCNNNNNNNGC 4 cut(s) 1004, 1016, 1303, 1543
NdeI CATATG 1 cut(s) 659
NdeII GATC 5 cut(s) 634, 654, 943, 1324, 1468
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 3 cut(s) 63, 835, 1334
NlaIV GGNNCC 5 cut(s) 123, 248, 591, 1047, 1226
NmuCI GTSAC 2 cut(s) 193, 731
NspI RCATGY 1 cut(s) 835
OliI CACNNNNGTG 1 cut(s) 28
PagI TCATGA 1 cut(s) 1330
PasI CCCWGGG 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 781, 1112, 1601
PflFI GACNNNGTC 1 cut(s) 958
PkrI GCNGC 5 cut(s) 7, 176, 528, 931, 1009
PleI GAGTC 6 cut(s) 63, 151, 460, 1175, 1196, 1277
PpsI GAGTC 6 cut(s) 63, 151, 460, 1175, 1196, 1277
PsiI TTATAA 3 cut(s) 407, 1209, 1636
Psp6I CCWGG 4 cut(s) 431, 736, 1162, 1270
PspEI GGTNACC 1 cut(s) 731
PspGI CCWGG 4 cut(s) 431, 736, 1162, 1270
PspN4I GGNNCC 5 cut(s) 123, 248, 591, 1047, 1226
PspPI GGNCC 2 cut(s) 1225, 1300
PstI CTGCAG 2 cut(s) 931, 1524
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 2 cut(s) 634, 1324
PsyI GACNNNGTC 1 cut(s) 958
PvuII CAGCTG 2 cut(s) 278, 1219
RsaI GTAC 3 cut(s) 1077, 1161, 1276
RsaNI GTAC 3 cut(s) 1076, 1160, 1275
RseI CAYNNNNRTG 3 cut(s) 28, 830, 1092
SaqAI TTAA 4 cut(s) 627, 867, 1319, 1382
SatI GCNGC 5 cut(s) 6, 175, 527, 930, 1008
Sau3AI GATC 5 cut(s) 634, 654, 943, 1324, 1468
Sau96I GGNCC 2 cut(s) 1225, 1300
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 6 cut(s) 63, 152, 461, 1176, 1197, 1277
ScrFI CCNGG 4 cut(s) 433, 738, 1164, 1272
SduI GDGCHC 6 cut(s) 35, 124, 487, 1050, 1539, 1592
SfaNI GCATC 2 cut(s) 603, 1072
SfcI CTRYAG 3 cut(s) 349, 927, 1520
SinI GGWCC 1 cut(s) 1300
SmiMI CAYNNNNRTG 3 cut(s) 28, 830, 1092
SmlI CTYRAG 3 cut(s) 583, 766, 1484
SmoI CTYRAG 3 cut(s) 583, 766, 1484
Sse9I AATT 8 cut(s) 240, 402, 608, 687, 717, 774, 1287, 1476
SsiI CCGC 3 cut(s) 5, 526, 1585
SspI AATATT 1 cut(s) 448
SspMI CTAG 5 cut(s) 171, 1011, 1079, 1410, 1532
StyD4I CCNGG 4 cut(s) 431, 736, 1162, 1270
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 4 cut(s) 30, 350, 647, 1264
TaiI ACGT 3 cut(s) 85, 607, 1161
TaqI TCGA 3 cut(s) 72, 102, 1198
TasI AATT 8 cut(s) 240, 402, 608, 687, 717, 774, 1287, 1476
TatI WGTACW 1 cut(s) 1075
TauI GCSGC 2 cut(s) 8, 529
TfiI GAWTC 3 cut(s) 781, 1112, 1601
Tru1I TTAA 4 cut(s) 627, 867, 1319, 1382
Tru9I TTAA 4 cut(s) 627, 867, 1319, 1382
TscAI CASTG 7 cut(s) 48, 202, 384, 517, 717, 984, 1524
TseFI GTSAC 2 cut(s) 193, 731
TseI GCWGC 3 cut(s) 174, 929, 1007
Tsp45I GTSAC 2 cut(s) 193, 731
TspDTI ATGAA 6 cut(s) 107, 201, 289, 984, 1122, 1319
TspGWI ACGGA 3 cut(s) 1433, 1576, 1604
TspRI CASTG 7 cut(s) 48, 202, 384, 517, 717, 984, 1524
Tth111I GACNNNGTC 1 cut(s) 958
VpaK11BI GGWCC 1 cut(s) 1300
XapI RAATTY 1 cut(s) 774
XceI RCATGY 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 5 cut(s) 171, 1011, 1079, 1410, 1532
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.