AT5G16740

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014378
Physical Location & Seq
Reverse (-)
5501044 .. 5502856
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16740.1

Sequence Viewer

Length: 1281 bp
ATGGTTTGTGTTGCATGTGTTGAGGAAAATAAAGGGTGTGAATGTGAGCATGAAAAACCGGTTAGAGAGTTGGTGTTGGAGGCGGCGTCTGAAAATAGTTCATTTCTTCACTCTGTTATCAACATGGTTGGTATGCTCATTGGACTTGGCCAACTATCTATGCCATACGCCGTTGAAAGCGGCGGTTGGATGTCAATCTTCCTCCTCATATCATTCGGAATCCTCACTACCTACACTTCCCACATCCTCGGAAAATGTATCCGCCGCAACCCCAAATCAAAATCCTACTCCGATATCGGCTACTCTGCCTTCGGCCGCCACGGCCGCCTCATCGTCTGCCTCTTCATCTACCTCGAAATCTTCATGGCCTTAGTGTCTTACACCATCTCCCTCCACGACAACATCTCCGCTGCTTTTCCCGCCACTTTTTCTAACCACGGCCATTTTCCCGCGGCCAAGCTGACGGCTGTGGCGGTGGCTATCGCCTTGCCGAGTTTGTGGATAAGAGATTTGTCTTCGATCTCGTTTCTTTCTTCCGGTGGTATTCTTATGTCGGCTATAATATTTGGGTCAGTGGTTTACACGGCCATCTTTGGAGGCGTTATTGATGATGGAAAGATTCCGGTGCTCCGGTTAGAGAATATTCCGACGGTTTCGGGAATCTATCTCTTTAGCTTCGGTGGACACATCGTATTTCCTAATCTCTATACTTCCATGAAAGATCCCTCCAAATTCACAAAGGTATCAATAGTAAGTTTTGCTACGGTAACGGCGTTATACGGAGCACTAGCCATCACAGGAGCGAAGATGTTCGGACCAAGTGTAAATTCCCAAATTACCCTCAGTCTTCCGAAACATTTAGTAGTTACCAAGATCGCTCTTTGGGCCACTGTACTAACCCCAATGACCAAATACGCCTTAGAGTTCGCTCCTTTGGCCATCCAGCTGGAACGTAGCCTTCCTTCGACCATGACTGATCGCACCAAGCTTGTGGCTCGCGGTCTTATGGGATCAGCTCTGTTGCTGGTCATTCTCGCATTGGCTTTAACCGTCCCTTACTTCGGTTATGTCTTGAGCCTCACGGGCTCTTTGGTTAGCGTCACAATCGCTGTGACATTACCGTCTGCTTTCTACTTGAAGATTTGCTGGGACGGGATGACGAAGTTCACACGAGCCGCCAATCTTGGTTTCGTTGTTCTCGGGTGTGTTCTTGGAGTTTTGGGATCTTTTGAATCATCAAAGTTGCTTGTCAAAGAACTCGTTCGCGTTCATGGAGGTTGA

Protein Analysis

426

Amino Acids

45.81

Weight (kDa)

9.0

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 32 - 409 1e-67 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16740
fragaria_vesca FvH4_4g12300
malus_domestica MD04G1027800.v1.1 MD13G1279400.v1.1
prunus_persica Prupe.1G177200_v2.0.a1
pyrus_communis pycom04g02440 pycom13g29390
rosa_chinensis RchiOBHm_Chr4g0410371
rosa_laevigata RLG00000008434
rosa_multiflora Rmu_sc0002111.1_g000022
rosa_roxburghii Rroxscaffold_5G00355180
rosa_rugosa Rorug04G0102200
rosa_samantha Rh4AG160300 Rh4BG159300 Rh4CG172400 Rh4DG155200
rosa_wichuraiana Rw4G013360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1120
AccII CGCG 3 cut(s) 452, 999, 1266
AclWI GGATC 3 cut(s) 716, 1018, 1231
AcoI YGGCCR 7 cut(s) 148, 313, 322, 439, 453, 585, 936
AcsI RAATTY 2 cut(s) 731, 826
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 1 cut(s) 894
AfiI CCNNNNNNNGG 1 cut(s) 1061
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 3 cut(s) 176, 1138, 1232
AjuI GAANNNNNNNTTGG 4 cut(s) 811, 843, 1172, 1204
AluBI AGCT 5 cut(s) 460, 675, 946, 988, 1016
AluI AGCT 5 cut(s) 460, 675, 946, 988, 1016
Alw21I GWGCWC 2 cut(s) 630, 787
AlwI GGATC 3 cut(s) 716, 1018, 1231
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 9 cut(s) 148, 313, 322, 366, 439, 453, 585, 885, 936
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 2 cut(s) 731, 826
AsiGI ACCGGT 1 cut(s) 58
Asp700I GAANNNNTTC 2 cut(s) 809, 1260
AspS9I GGNCC 2 cut(s) 815, 885
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 1 cut(s) 815
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BalI TGGCCA 2 cut(s) 150, 938
BanII GRGCYC 1 cut(s) 1088
BarI GAAGNNNNNNTAC 2 cut(s) 946, 978
BauI CACGAG 1 cut(s) 1170
BbsI GAAGAC 2 cut(s) 507, 839
Bbv12I GWGCWC 2 cut(s) 630, 787
BbvI GCAGC 1 cut(s) 397
BccI CCATC 5 cut(s) 392, 596, 605, 800, 947
BceAI ACGGC 6 cut(s) 155, 337, 454, 480, 600, 786
BciVI GTATCC 1 cut(s) 269
BfaI CTAG 1 cut(s) 788
BfuI GTATCC 1 cut(s) 269
BglI GCCNNNNNGGC 2 cut(s) 321, 1083
BisI GCNGC 8 cut(s) 84, 181, 265, 316, 325, 411, 453, 1176
BlsI GCNGC 8 cut(s) 85, 182, 266, 317, 326, 412, 454, 1177
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 1199
BmgT120I GGNCC 2 cut(s) 815, 885
BpiI GAAGAC 2 cut(s) 507, 839
BpuEI CTTGAG 1 cut(s) 1093
BsaBI GATNNNNATC 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 247, 319, 436, 450
BsaWI WCCGGW 4 cut(s) 58, 536, 622, 630
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 371, 389, 401, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 1061
Bse118I RCCGGY 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 194
BseDI CCNNGG 4 cut(s) 247, 319, 436, 450
BseGI GGATG 4 cut(s) 195, 243, 939, 1161
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 1 cut(s) 1061
BseMII CTCAG 1 cut(s) 856
BseRI GAGGAG 1 cut(s) 194
BseX3I CGGCCG 2 cut(s) 313, 322
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 1146
Bsh1236I CGCG 3 cut(s) 452, 999, 1266
Bsh1285I CGRYCG 2 cut(s) 316, 325
BshFI GGCC 9 cut(s) 150, 315, 324, 368, 441, 455, 587, 887, 938
BshTI ACCGGT 1 cut(s) 58
BsiEI CGRYCG 2 cut(s) 316, 325
BsiHKAI GWGCWC 2 cut(s) 630, 787
BsiHKCI CYCGRG 1 cut(s) 1199
BsiSI CCGG 4 cut(s) 59, 537, 623, 631
BslFI GGGAC 2 cut(s) 1037, 1163
BslI CCNNNNNNNGG 1 cut(s) 1061
BsmFI GGGAC 2 cut(s) 1037, 1163
BsnI GGCC 9 cut(s) 150, 315, 324, 368, 441, 455, 587, 887, 938
BsoBI CYCGRG 1 cut(s) 1199
Bsp1286I GDGCHC 3 cut(s) 630, 787, 1088
Bsp143I GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
BspANI GGCC 9 cut(s) 150, 315, 324, 368, 441, 455, 587, 887, 938
BspCNI CTCAG 1 cut(s) 855
BspFNI CGCG 3 cut(s) 452, 999, 1266
BspPI GGATC 3 cut(s) 716, 1018, 1231
BsrFI RCCGGY 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 4 cut(s) 247, 319, 436, 450
BssMI GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
BssNI GRCGYC 1 cut(s) 86
BssSI CACGAG 1 cut(s) 1170
Bst2BI CACGAG 1 cut(s) 1170
Bst4CI ACNGT 5 cut(s) 652, 766, 892, 1051, 1122
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 997
BstDEI CTNAG 3 cut(s) 370, 842, 919
BstDSI CCRYGG 3 cut(s) 319, 436, 450
BstF5I GGATG 4 cut(s) 195, 243, 939, 1161
BstFNI CGCG 3 cut(s) 452, 999, 1266
BstKTI GATC 6 cut(s) 522, 724, 876, 979, 1013, 1226
BstMBI GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
BstMCI CGRYCG 2 cut(s) 316, 325
BstMWI GCNNNNNNNGC 6 cut(s) 321, 324, 419, 884, 935, 1083
BstNSI RCATGY 1 cut(s) 18
BstUI CGCG 3 cut(s) 452, 999, 1266
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 2 cut(s) 507, 839
BstX2I RGATCY 2 cut(s) 721, 1223
BstXI CCANNNNNNTGG 2 cut(s) 946, 991
BstYI RGATCY 2 cut(s) 721, 1223
BstZI CGGCCG 2 cut(s) 313, 322
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 9 cut(s) 150, 315, 324, 368, 441, 455, 587, 887, 938
BtgI CCRYGG 3 cut(s) 319, 436, 450
BtsCI GGATG 4 cut(s) 195, 243, 939, 1161
BtsIMutI CAGTG 2 cut(s) 579, 888
Cac8I GCNNGC 1 cut(s) 997
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 815, 885
Cfr42I CCGCGG 1 cut(s) 453
CseI GACGC 2 cut(s) 75, 1087
Csp6I GTAC 1 cut(s) 893
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 7 cut(s) 15, 50, 124, 364, 715, 970, 1271
CviQI GTAC 1 cut(s) 893
DdeI CTNAG 3 cut(s) 370, 842, 919
DpnI GATC 6 cut(s) 521, 723, 875, 978, 1012, 1225
DpnII GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
DrdI GACNNNNNNGTC 1 cut(s) 1120
DseDI GACNNNNNNGTC 1 cut(s) 1120
EaeI YGGCCR 7 cut(s) 148, 313, 322, 439, 453, 585, 936
EagI CGGCCG 2 cut(s) 313, 322
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 1 cut(s) 251
EclXI CGGCCG 2 cut(s) 313, 322
Eco24I GRGCYC 1 cut(s) 1088
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 815
Eco52I CGGCCG 2 cut(s) 313, 322
Eco88I CYCGRG 1 cut(s) 1199
EcoRV GATATC 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 1088
FaeI CATG 7 cut(s) 18, 53, 127, 367, 718, 973, 1274
FaqI GGGAC 2 cut(s) 1037, 1163
FatI CATG 7 cut(s) 14, 49, 123, 363, 714, 969, 1270
FauI CCCGC 2 cut(s) 427, 457
Fnu4HI GCNGC 8 cut(s) 84, 181, 265, 316, 325, 411, 453, 1176
FokI GGATG 4 cut(s) 202, 230, 926, 1168
FriOI GRGCYC 1 cut(s) 1088
Fsp4HI GCNGC 8 cut(s) 84, 181, 265, 316, 325, 411, 453, 1176
FspBI CTAG 1 cut(s) 788
GluI GCNGC 8 cut(s) 84, 181, 265, 316, 325, 411, 453, 1176
GsaI CCCAGC 1 cut(s) 1150
HaeIII GGCC 9 cut(s) 150, 315, 324, 368, 441, 455, 587, 887, 938
HapII CCGG 4 cut(s) 59, 537, 623, 631
HgaI GACGC 2 cut(s) 75, 1087
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 7 cut(s) 18, 53, 127, 367, 718, 973, 1274
HindIII AAGCTT 1 cut(s) 986
HinfI GANTC 4 cut(s) 219, 619, 660, 1232
HpaII CCGG 4 cut(s) 59, 537, 623, 631
Hpy166II GTNNAC 3 cut(s) 580, 683, 1167
Hpy188I TCNGA 7 cut(s) 91, 218, 251, 292, 648, 815, 852
Hpy188III TCNNGA 2 cut(s) 657, 1072
Hpy8I GTNNAC 3 cut(s) 580, 683, 1167
Hpy99I CGWCG 1 cut(s) 652
HpyAV CCTTC 3 cut(s) 319, 968, 972
HpyCH4III ACNGT 5 cut(s) 652, 766, 892, 1051, 1122
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 1 cut(s) 14
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 324, 419, 884, 935, 1083
HpyF3I CTNAG 3 cut(s) 370, 842, 919
HpySE526I ACGT 1 cut(s) 952
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 7 cut(s) 18, 53, 127, 367, 718, 973, 1274
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
LmnI GCTCC 4 cut(s) 633, 782, 800, 934
LpnPI CCDG 9 cut(s) 72, 550, 636, 644, 783, 932, 956, 1010, 1132
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 1 cut(s) 788
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 4 cut(s) 766, 865, 1099, 1111
MalI GATC 6 cut(s) 521, 723, 875, 978, 1012, 1225
MboI GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
MboII GAAGA 9 cut(s) 98, 190, 334, 352, 507, 525, 817, 839, 1150
MflI RGATCY 2 cut(s) 721, 1223
MhlI GDGCHC 3 cut(s) 630, 787, 1088
MlsI TGGCCA 2 cut(s) 150, 938
MluCI AATT 3 cut(s) 731, 826, 834
MluNI TGGCCA 2 cut(s) 150, 938
MmeI TCCRAC 3 cut(s) 57, 167, 671
Mox20I TGGCCA 2 cut(s) 150, 938
MroXI GAANNNNTTC 2 cut(s) 809, 1260
MscI TGGCCA 2 cut(s) 150, 938
MseI TTAA 1 cut(s) 1046
Msp20I TGGCCA 2 cut(s) 150, 938
MspA1I CMGCKG 3 cut(s) 410, 452, 946
MspI CCGG 4 cut(s) 59, 537, 623, 631
MvnI CGCG 3 cut(s) 452, 999, 1266
MwoI GCNNNNNNNGC 6 cut(s) 321, 324, 419, 884, 935, 1083
NdeII GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
NlaIII CATG 7 cut(s) 18, 53, 127, 367, 718, 973, 1274
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 2 cut(s) 1099, 1111
NspI RCATGY 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 770
PdmI GAANNNNTTC 2 cut(s) 809, 1260
PfeI GAWTC 4 cut(s) 219, 619, 660, 1232
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 8 cut(s) 85, 182, 266, 317, 326, 412, 454, 1177
PspFI CCCAGC 1 cut(s) 1146
PspPI GGNCC 2 cut(s) 815, 885
PsuI RGATCY 2 cut(s) 721, 1223
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 1 cut(s) 894
RsaNI GTAC 1 cut(s) 893
SacII CCGCGG 1 cut(s) 453
SaqAI TTAA 1 cut(s) 1046
SatI GCNGC 8 cut(s) 84, 181, 265, 316, 325, 411, 453, 1176
Sau3AI GATC 6 cut(s) 519, 721, 873, 976, 1010, 1223
Sau96I GGNCC 2 cut(s) 815, 885
SduI GDGCHC 3 cut(s) 630, 787, 1088
SfiI GGCCNNNNNGGCC 1 cut(s) 321
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SinI GGWCC 1 cut(s) 815
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
Sse9I AATT 3 cut(s) 731, 826, 834
SspI AATATT 2 cut(s) 564, 643
SspMI CTAG 1 cut(s) 788
TaaI ACNGT 5 cut(s) 652, 766, 892, 1051, 1122
TaiI ACGT 1 cut(s) 955
TaqI TCGA 3 cut(s) 354, 518, 965
TasI AATT 3 cut(s) 731, 826, 834
TatI WGTACW 1 cut(s) 892
TauI GCSGC 7 cut(s) 86, 183, 267, 318, 327, 455, 1178
TfiI GAWTC 4 cut(s) 219, 619, 660, 1232
Tru1I TTAA 1 cut(s) 1046
Tru9I TTAA 1 cut(s) 1046
TscAI CASTG 2 cut(s) 579, 895
TseFI GTSAC 2 cut(s) 1099, 1111
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 2 cut(s) 1099, 1111
TspDTI ATGAA 6 cut(s) 66, 90, 334, 352, 731, 1259
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 2 cut(s) 579, 895
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 2 cut(s) 731, 826
XceI RCATGY 1 cut(s) 18
XmnI GAANNNNTTC 2 cut(s) 809, 1260
XspI CTAG 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.