Rroxscaffold_5G00355180

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014378
Physical Location & Seq
Reverse (-)
33789863 .. 33791986
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355180.1

Sequence Viewer

Length: 1395 bp
ATGTTCAAGTGGTTTCAATCAAAGTGTCTTCCGCACCAAAACCAAGTTGCTAGTGAGTCGGTGCACGGCAGGAACATGGCTCTGAATGAGCTAAGCTGCAGTGTTTTCCTGGAGGAAAACAAAGTGTGCAAATGTGAACAAAGTTTCGGGAGCTGTAAGAAAGTCGACTCAGATGTTAGCGATGACGAGCAACATGGAGAGTCCAATAATTCTGTTACTCATGCTGTGATCAACATGACTGGGATGCTCATAGGTTTGGGGCAGTTATCAACTCCATATGCCATAGAATCTGGAGGGTGGGTTTCTGCATTCTTAATGATAGGACTTGGTGTTGTATGTGCTTATTGTTCTCATCTACTCGGGAAATGCCTAGACAAACATCCCAAGTCAAGAAGCTACACAGATATTGGACAGAATGCATTTGGAACCAAAGGAAAACTCCTAGCAGCAACCTTCATTTACTTGGAGATCTTCATGGCCCTTGTGTCCTACACCATCTCATTGCATGACAACATAAACAGAGTTTTCTCGGGGACCCAAGCTCAGATTCCATGGGCCAAGTTATCGAGATCTCAGCTCCTGACGTTGGTGGCGGTTCTGATTGCTCTGCCGAGTCTGTGGTTGAGAGATCTCTCTTCCATATCATTCCTTTCCTTTGGTGGTGTTCTCATGTCAGTTGCCATTTTTTCATCAGTGGCATGCACAGGCATTTTTGGAGGGGTGAAAGCTAATCATAGTATACCAGTCCTTCAGATTCAAAACATTCCTGCAATATCTGGCCTTTATATCTTCAGCTATGCAGGACATATTGTCTTTCCTAATTTATACAAGGCCATGAAAGATCCTTCCAAGTTCACGAAGGTATCTATAATAAGCTTCAGCTTAGTCACTCTGCTTTACGGCGCTCTAGCCTTCATGGGTGCCAAGTTGTTTGGTCCCCAAGTAAATCCCCAAATCACTCTAAGCATGCCTCCCCATCTTATTGTTACAAAGATTGCACTATGGGCCACAGTGCTCACACCAATGACCAAATATGCTTTGGAATTTGCACCAATGGCAATCCAGCTGGAGTATAATCTCCCAGATTCCATGAGTTCAAGAACAAAGTTGATTGTGAGAGGTACTGTTGGTTCCTTTCTACTTCTGGTGATTCTCGCACTAGCGCTGTCAGTTCCATATTTTCAGTATGTTCTCAGCCTCACCGGATCCCTTGTAAGCATTGCTATTTGTGTCATTTTCCCTTGTGCCTTCTACCTCAAGATCTGTTGGGCTCAGATATCAAGGCCTCTTCTGGTCCTAAACTTCACCTTGATTGCATTTGGGTTTGTTCTTGGAGTGTTTGGAACCATTTCCTCATCAAAGTTGCTCATGCAGAATCTGAGCAGAGCTCACTGA

Protein Analysis

464

Amino Acids

50.61

Weight (kDa)

9.05

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 69 - 451 2.2e-65 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16740
fragaria_vesca FvH4_4g12300
malus_domestica MD04G1027800.v1.1 MD13G1279400.v1.1
prunus_persica Prupe.1G177200_v2.0.a1
pyrus_communis pycom04g02440 pycom13g29390
rosa_chinensis RchiOBHm_Chr4g0410371
rosa_laevigata RLG00000008434
rosa_multiflora Rmu_sc0002111.1_g000022
rosa_roxburghii Rroxscaffold_5G00355180
rosa_rugosa Rorug04G0102200
rosa_samantha Rh4AG160300 Rh4BG159300 Rh4CG172400 Rh4DG155200
rosa_wichuraiana Rw4G013360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 920
AccI GTMKAC 2 cut(s) 165, 739
AciI CCGC 2 cut(s) 32, 593
AclWI GGATC 3 cut(s) 836, 1200, 1213
AcsI RAATTY 1 cut(s) 1043
AcuI CTGAAG 3 cut(s) 734, 775, 862
AfaI GTAC 1 cut(s) 1123
AfeI AGCGCT 1 cut(s) 1164
AfiI CCNNNNNNNGG 1 cut(s) 586
AgsI TTSAA 4 cut(s) 7, 17, 758, 1098
AhdI GACNNNNNGTC 1 cut(s) 809
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
Alw21I GWGCWC 3 cut(s) 66, 1017, 1390
Alw44I GTGCAC 1 cut(s) 62
AlwI GGATC 3 cut(s) 836, 1200, 1213
AlwNI CAGNNNCTG 2 cut(s) 580, 1378
Ama87I CYCGRG 2 cut(s) 359, 529
Aor51HI AGCGCT 1 cut(s) 1164
AoxI GGCC 6 cut(s) 477, 555, 778, 831, 1005, 1283
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 96, 446
ApoI RAATTY 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 1348
AspLEI GCGC 2 cut(s) 905, 1165
AspS9I GGNCC 6 cut(s) 478, 534, 555, 935, 1005, 1294
AsuHPI GGTGA 4 cut(s) 733, 1159, 1192, 1297
AvaI CYCGRG 2 cut(s) 359, 529
AvaII GGWCC 3 cut(s) 534, 935, 1294
BaeGI GKGCMC 1 cut(s) 66
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BamHI GGATCC 1 cut(s) 1205
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 2 cut(s) 1273, 1390
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 3 cut(s) 66, 1017, 1390
BbvI GCAGC 2 cut(s) 83, 458
BccI CCATC 2 cut(s) 503, 984
BceAI ACGGC 2 cut(s) 82, 916
BciT130I CCWGG 1 cut(s) 110
BclI TGATCA 1 cut(s) 228
BfaI CTAG 5 cut(s) 51, 371, 443, 908, 1160
BfmI CTRYAG 1 cut(s) 97
BfoI RGCGCY 2 cut(s) 906, 1166
BglII AGATCT 4 cut(s) 468, 569, 628, 1260
BisI GCNGC 2 cut(s) 97, 447
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 2 cut(s) 98, 448
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 3 cut(s) 534, 935, 1294
BmeRI GACNNNNNGTC 1 cut(s) 809
BmeT110I CYCGRG 2 cut(s) 359, 529
BmgT120I GGNCC 6 cut(s) 478, 534, 555, 935, 1005, 1294
BmiI GGNNCC 8 cut(s) 427, 535, 536, 922, 937, 1132, 1207, 1345
BmrFI CCNGG 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 234
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 20
BplI GAGNNNNNCTC 1 cut(s) 1372
BpmI CTGGAG 3 cut(s) 131, 312, 1088
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 1241
BsaJI CCNNGG 1 cut(s) 551
BsaWI WCCGGW 1 cut(s) 1202
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse1I ACTGG 2 cut(s) 244, 743
Bse3DI GCAATG 2 cut(s) 500, 1218
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 2 cut(s) 249, 379
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMI GCAATG 2 cut(s) 500, 1218
BseMII CTCAG 6 cut(s) 183, 557, 587, 1207, 1286, 1370
BseNI ACTGG 2 cut(s) 244, 743
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 2 cut(s) 83, 458
BshFI GGCC 6 cut(s) 479, 557, 780, 833, 1007, 1285
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 3 cut(s) 66, 1017, 1390
BsiHKCI CYCGRG 2 cut(s) 359, 529
BsiSI CCGG 1 cut(s) 1203
BslFI GGGAC 2 cut(s) 547, 921
BslI CCNNNNNNNGG 1 cut(s) 586
BsmFI GGGAC 2 cut(s) 547, 921
BsmI GAATGC 2 cut(s) 308, 421
BsnI GGCC 6 cut(s) 479, 557, 780, 833, 1007, 1285
BsoBI CYCGRG 2 cut(s) 359, 529
Bsp1286I GDGCHC 4 cut(s) 66, 1017, 1273, 1390
Bsp143I GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
Bsp1720I GCTNAGC 1 cut(s) 92
Bsp19I CCATGG 1 cut(s) 551
BspACI CCGC 2 cut(s) 32, 593
BspANI GGCC 6 cut(s) 479, 557, 780, 833, 1007, 1285
BspCNI CTCAG 6 cut(s) 182, 556, 586, 1206, 1285, 1371
BspLI GGNNCC 8 cut(s) 427, 535, 536, 922, 937, 1132, 1207, 1345
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 3 cut(s) 836, 1200, 1213
BspT107I GGYRCC 1 cut(s) 920
BsrDI GCAATG 2 cut(s) 500, 1218
BsrI ACTGG 2 cut(s) 244, 743
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
BssNAI GTATAC 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 551
Bst1107I GTATAC 1 cut(s) 740
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 1012, 1126
Bst6I CTCTTC 2 cut(s) 640, 1293
BstC8I GCNNGC 2 cut(s) 700, 968
BstDEI CTNAG 9 cut(s) 92, 169, 543, 573, 883, 962, 1193, 1272, 1379
BstDSI CCRYGG 1 cut(s) 551
BstF5I GGATG 2 cut(s) 249, 379
BstH2I RGCGCY 2 cut(s) 906, 1166
BstHHI GCGC 2 cut(s) 905, 1165
BstKTI GATC 7 cut(s) 231, 471, 572, 631, 844, 1208, 1263
BstMBI GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
BstMWI GCNNNNNNNGC 2 cut(s) 1004, 1055
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 2 cut(s) 702, 970
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 83, 458
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 6 cut(s) 468, 569, 628, 841, 1205, 1260
BstYI RGATCY 6 cut(s) 468, 569, 628, 841, 1205, 1260
BstZ17I GTATAC 1 cut(s) 740
BsuRI GGCC 6 cut(s) 479, 557, 780, 833, 1007, 1285
BtgI CCRYGG 1 cut(s) 551
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 2 cut(s) 249, 379
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 4 cut(s) 106, 699, 1017, 1390
Cac8I GCNNGC 2 cut(s) 700, 968
CaiI CAGNNNCTG 2 cut(s) 580, 1378
CfoI GCGC 2 cut(s) 905, 1165
Cfr13I GGNCC 6 cut(s) 478, 534, 555, 935, 1005, 1294
Csp6I GTAC 1 cut(s) 1122
CviQI GTAC 1 cut(s) 1122
DdeI CTNAG 9 cut(s) 92, 169, 543, 573, 883, 962, 1193, 1272, 1379
DpnI GATC 7 cut(s) 230, 470, 571, 630, 843, 1207, 1262
DpnII GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
DriI GACNNNNNGTC 1 cut(s) 809
Eam1104I CTCTTC 2 cut(s) 640, 1293
Eam1105I GACNNNNNGTC 1 cut(s) 809
EarI CTCTTC 2 cut(s) 640, 1293
Ecl136II GAGCTC 1 cut(s) 1388
Eco130I CCWWGG 1 cut(s) 551
Eco147I AGGCCT 1 cut(s) 1285
Eco24I GRGCYC 2 cut(s) 1273, 1390
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 3 cut(s) 534, 935, 1294
Eco47III AGCGCT 1 cut(s) 1164
Eco53kI GAGCTC 1 cut(s) 1388
Eco57I CTGAAG 3 cut(s) 734, 775, 862
Eco88I CYCGRG 2 cut(s) 359, 529
EcoICRI GAGCTC 1 cut(s) 1388
EcoO109I RGGNCCY 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 108
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 1 cut(s) 551
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 2 cut(s) 1273, 1390
ErhI CCWWGG 1 cut(s) 551
FaqI GGGAC 2 cut(s) 547, 921
FauNDI CATATG 1 cut(s) 277
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 2 cut(s) 165, 739
Fnu4HI GCNGC 2 cut(s) 97, 447
FokI GGATG 2 cut(s) 256, 366
FriOI GRGCYC 2 cut(s) 1273, 1390
Fsp4HI GCNGC 2 cut(s) 97, 447
FspBI CTAG 5 cut(s) 51, 371, 443, 908, 1160
GlaI GCGC 2 cut(s) 904, 1164
GluI GCNGC 2 cut(s) 97, 447
GsuI CTGGAG 3 cut(s) 131, 312, 1088
HaeII RGCGCY 2 cut(s) 906, 1166
HaeIII GGCC 6 cut(s) 479, 557, 780, 833, 1007, 1285
HapII CCGG 1 cut(s) 1203
HhaI GCGC 2 cut(s) 905, 1165
Hin6I GCGC 2 cut(s) 903, 1163
HinP1I GCGC 2 cut(s) 903, 1163
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 874
HpaII CCGG 1 cut(s) 1203
HphI GGTGA 4 cut(s) 733, 1159, 1192, 1297
Hpy166II GTNNAC 5 cut(s) 64, 137, 166, 740, 855
Hpy188I TCNGA 7 cut(s) 84, 172, 546, 600, 753, 1275, 1380
Hpy188III TCNNGA 9 cut(s) 148, 291, 361, 390, 567, 580, 856, 1098, 1258
Hpy8I GTNNAC 5 cut(s) 64, 137, 166, 740, 855
HpyAV CCTTC 6 cut(s) 463, 758, 853, 855, 922, 1258
HpyCH4III ACNGT 2 cut(s) 1012, 1126
HpyCH4IV ACGT 1 cut(s) 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 1004, 1055
HpyF3I CTNAG 9 cut(s) 92, 169, 543, 573, 883, 962, 1193, 1272, 1379
HpySE526I ACGT 1 cut(s) 584
HspAI GCGC 2 cut(s) 903, 1163
KflI GGGWCCC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
LmnI GCTCC 2 cut(s) 150, 582
Lsp1109I GCAGC 2 cut(s) 83, 458
LweI GCATC 1 cut(s) 234
MaeI CTAG 5 cut(s) 51, 371, 443, 908, 1160
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 3 cut(s) 214, 886, 985
MalI GATC 7 cut(s) 230, 470, 571, 630, 843, 1207, 1262
MboI GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
MboII GAAGA 5 cut(s) 20, 463, 627, 781, 1280
MflI RGATCY 6 cut(s) 468, 569, 628, 841, 1205, 1260
MhlI GDGCHC 4 cut(s) 66, 1017, 1273, 1390
MluCI AATT 3 cut(s) 208, 820, 1043
MlyI GAGTC 4 cut(s) 65, 161, 209, 622
MnlI CCTC 9 cut(s) 106, 287, 710, 981, 1112, 1208, 1265, 1296, 1363
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 1348
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 1022
MspA1I CMGCKG 1 cut(s) 1066
MspI CCGG 1 cut(s) 1203
MspR9I CCNGG 1 cut(s) 110
Mva1269I GAATGC 2 cut(s) 308, 421
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 1004, 1055
NcoI CCATGG 1 cut(s) 551
NdeI CATATG 1 cut(s) 277
NdeII GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
NlaIV GGNNCC 8 cut(s) 427, 535, 536, 922, 937, 1132, 1207, 1345
NmeAIII GCCGAG 1 cut(s) 636
NmuCI GTSAC 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 2 cut(s) 702, 970
PaeI GCATGC 2 cut(s) 702, 970
PceI AGGCCT 1 cut(s) 1285
PctI GAATGC 2 cut(s) 308, 421
PdmI GAANNNNTTC 1 cut(s) 1348
PfeI GAWTC 6 cut(s) 287, 547, 754, 1085, 1150, 1375
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 2 cut(s) 98, 448
PleI GAGTC 4 cut(s) 64, 161, 208, 621
PpsI GAGTC 4 cut(s) 64, 161, 208, 621
PpuMI RGGWCCY 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 1390
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 8 cut(s) 427, 535, 536, 922, 937, 1132, 1207, 1345
PspPI GGNCC 6 cut(s) 478, 534, 555, 935, 1005, 1294
PspPPI RGGWCCY 1 cut(s) 534
PsrI GAACNNNNNNTAC 2 cut(s) 1114, 1146
PstI CTGCAG 1 cut(s) 101
PstNI CAGNNNCTG 2 cut(s) 580, 1378
PsuI RGATCY 6 cut(s) 468, 569, 628, 841, 1205, 1260
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 1 cut(s) 1123
RsaNI GTAC 1 cut(s) 1122
RseI CAYNNNNRTG 1 cut(s) 1022
SacI GAGCTC 1 cut(s) 1390
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 2 cut(s) 97, 447
Sau3AI GATC 7 cut(s) 228, 468, 569, 628, 841, 1205, 1260
Sau96I GGNCC 6 cut(s) 478, 534, 555, 935, 1005, 1294
SchI GAGTC 4 cut(s) 65, 161, 209, 622
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 4 cut(s) 66, 1017, 1273, 1390
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 3 cut(s) 534, 935, 1294
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 1 cut(s) 1256
SmoI CTYRAG 1 cut(s) 1256
SphI GCATGC 2 cut(s) 702, 970
Sse9I AATT 3 cut(s) 208, 820, 1043
SseBI AGGCCT 1 cut(s) 1285
SsiI CCGC 2 cut(s) 32, 593
SspMI CTAG 5 cut(s) 51, 371, 443, 908, 1160
SstI GAGCTC 1 cut(s) 1390
StuI AGGCCT 1 cut(s) 1285
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 2 cut(s) 1012, 1126
TaiI ACGT 1 cut(s) 587
TaqI TCGA 2 cut(s) 165, 566
TasI AATT 3 cut(s) 208, 820, 1043
TfiI GAWTC 6 cut(s) 287, 547, 754, 1085, 1150, 1375
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 3 cut(s) 106, 699, 1017
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 2 cut(s) 96, 446
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 5 cut(s) 445, 463, 678, 851, 904
TspRI CASTG 3 cut(s) 106, 699, 1017
VneI GTGCAC 1 cut(s) 62
VpaK11BI GGWCC 3 cut(s) 534, 935, 1294
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 2 cut(s) 702, 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XmiI GTMKAC 2 cut(s) 165, 739
XmnI GAANNNNTTC 1 cut(s) 1348
XspI CTAG 5 cut(s) 51, 371, 443, 908, 1160
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.