pycom13g29390

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014378
Physical Location & Seq
Forward (+)
26159179 .. 26161551
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g29390.1

Sequence Viewer

Length: 1446 bp
ATGAACATGTGGAACAAATTGTTTGGGAAATCAAAATCAAACTGCCTCCCACACCAAAATCAAGTTATTGGGAGCGATGCAATGCAGCGTGCGAAGTTGGCGTTGCAACAGCTGAGCTGCAATGTATGTCTGGAGGAAAACAAAGTTTGTAAGTGCGACCAAAGCTTTGAGAGCTGCAAGAGAGTCGCGGGTGCTGATGAGCACCATGCAGAGGCCAACAATTCTGTTACTCATTCTGTTATCAACATGACAGGGATGCTCATAGGTTTGGGACAGTTATCAACTCCATATGCCTTAGAAACTGGGGGTTGGTCTTCTGCATTCCTATTAATAGGACTTGGGATAATATGTGCATATTGTTCTCATCTCCTTGGAAAATGTCTTGACAAACATCCCAAGTCAAGAACCTACACAGATATTGGACACAATGCATTTGGATCCAAAGGAAAAATTCTAGCAGCATCCTTCATTTACATGGAGATCTTCATGGCTCTTGTATCCTACACCATCTCTTTGCATGACAATCTCGCCACAGTGTTCCATGGGATCCAACTCAAAGTTTCGTGGGCTAAGTTTCCAAAATCTCAGCTCTTAACCTTCATTGCCGTCTTGGTAGCTCTCCCCAGTCTTTGGTTGAGAGATCTCTCTTCCATATCTTTCCTTTCCTTCGGTGGTGTTCTCATGTCACTCGTCATTTTTATATCAGTGGCATGCACCGCCATTTTCGGAGGGGTGAAATTGAACCACACCATACCAGCCCTCCAGATTCATAACATTCCTGCAATATCTGGCCTGTATATCTTCAGCTTTGCAGGACATATTGTTTTCCCCAACTTATACAAGGCCATGAAGGACCCATCCAAGTTCACCAAGGTATCATTAATAAGCTTCACTTTAGTGACAATACTTTACACATCCCTAGCATTCATGGGAGCCAAGTTGTTTGGTCCTCAGGTGAATCCTCAAATCACTCTAAGCATGCCTCCACATCTTATTGTGACAAAGATTGCTTTGTGGGCAACTGTGCTGACACCAATGACCAAATATGCTCTTGAATTTGCACCGATGGCTATCCAGCTTGAGCATAACCTTCCTCAATCTTTCAGTTCCAGAACAAAGTTGCTCATCCGGGGCAGCGTCGGTTCAGTGCTACTTCTATTGATTCTAGTACTAGCTCTCTCTGTCCCATATTTTGAGTATGTTCTCAGCCTCACTGGATCGCTTGTAAGTACTGGCATTTGTGTCATTTTCCCTTGTGCCTTCTACCTCAAGATATGCTGGGGTCAAATTTCAAGGCCTCTCCTGGTCCTCAACCTCACTCTAATTGCATTTGGCACCCTCCTAGGGATAGTTGGAACCATTTCCTCATCAAAGTTGCTCCTAAAAAACCTCAGCAGAGCTCACAGCCTGAAGAATATGAACCAAGTATCTCATACATGCCAATAA

Protein Analysis

482

Amino Acids

52.52

Weight (kDa)

9.35

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 74 - 455 1.1e-63 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16740
fragaria_vesca FvH4_4g12300
malus_domestica MD04G1027800.v1.1 MD13G1279400.v1.1
prunus_persica Prupe.1G177200_v2.0.a1
pyrus_communis pycom04g02440 pycom13g29390
rosa_chinensis RchiOBHm_Chr4g0410371
rosa_laevigata RLG00000008434
rosa_multiflora Rmu_sc0002111.1_g000022
rosa_roxburghii Rroxscaffold_5G00355180
rosa_rugosa Rorug04G0102200
rosa_samantha Rh4AG160300 Rh4BG159300 Rh4CG172400 Rh4DG155200
rosa_wichuraiana Rw4G013360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1334
AccB7I CCANNNNNTGG 1 cut(s) 630
AccII CGCG 1 cut(s) 188
AciI CCGC 2 cut(s) 188, 717
AclWI GGATC 5 cut(s) 432, 445, 541, 554, 1225
AcsI RAATTY 3 cut(s) 450, 1055, 1287
AcuI CTGAAG 2 cut(s) 787, 1430
AfaI GTAC 2 cut(s) 1170, 1231
AfiI CCNNNNNNNGG 3 cut(s) 211, 630, 1344
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 3 cut(s) 742, 1055, 1293
AjnI CCWGG 1 cut(s) 1302
AleI CACNNNNGTG 1 cut(s) 896
Alw21I GWGCWC 2 cut(s) 204, 1402
AlwI GGATC 5 cut(s) 432, 445, 541, 554, 1225
AoxI GGCC 4 cut(s) 213, 790, 843, 1295
ApeKI GCWGC 5 cut(s) 85, 117, 174, 458, 1134
ApoI RAATTY 3 cut(s) 450, 1055, 1287
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AseI ATTAAT 2 cut(s) 329, 881
Asp700I GAANNNNTTC 1 cut(s) 1360
AspA2I CCTAGG 1 cut(s) 1342
AspS9I GGNCC 3 cut(s) 853, 947, 1306
AsuC2I CCSGG 1 cut(s) 1130
AsuHPI GGTGA 3 cut(s) 745, 859, 967
AvaII GGWCC 3 cut(s) 853, 947, 1306
AvrII CCTAGG 1 cut(s) 1342
AxyI CCTNAGG 1 cut(s) 951
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BamHI GGATCC 2 cut(s) 437, 546
BanI GGYRCC 1 cut(s) 1334
BanII GRGCYC 1 cut(s) 1402
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 2 cut(s) 204, 1402
BbvCI CCTCAGC 1 cut(s) 1391
BbvI GCAGC 5 cut(s) 97, 104, 161, 470, 1146
BccI CCATC 3 cut(s) 515, 865, 1060
BceAI ACGGC 1 cut(s) 590
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 1304
BciVI GTATCC 1 cut(s) 508
BcnI CCSGG 1 cut(s) 1130
BfaI CTAG 5 cut(s) 455, 920, 1166, 1172, 1343
BfuI GTATCC 1 cut(s) 508
BglII AGATCT 2 cut(s) 480, 640
BisI GCNGC 5 cut(s) 86, 118, 175, 459, 1135
BlnI CCTAGG 1 cut(s) 1342
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 5 cut(s) 87, 119, 176, 460, 1136
BmcAI AGTACT 2 cut(s) 1170, 1231
Bme1390I CCNGG 2 cut(s) 1130, 1304
Bme18I GGWCC 3 cut(s) 853, 947, 1306
BmgT120I GGNCC 3 cut(s) 853, 947, 1306
BmiI GGNNCC 6 cut(s) 439, 548, 855, 934, 1336, 1357
BmrFI CCNGG 2 cut(s) 1130, 1304
BmrI ACTGGG 2 cut(s) 312, 618
BmsI GCATC 3 cut(s) 67, 246, 470
BmuI ACTGGG 2 cut(s) 312, 618
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 2 cut(s) 152, 746
Bpu10I CCTNAGC 1 cut(s) 1391
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 2 cut(s) 1100, 1253
BpuMI CCSGG 1 cut(s) 1130
BsaBI GATNNNNATC 1 cut(s) 1070
BsaJI CCNNGG 5 cut(s) 370, 541, 870, 1129, 1342
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 630, 1344
Bse1I ACTGG 4 cut(s) 307, 624, 1219, 1237
Bse21I CCTNAGG 1 cut(s) 951
Bse3DI GCAATG 3 cut(s) 87, 127, 600
Bse8I GATNNNNATC 1 cut(s) 1070
BseBI CCWGG 1 cut(s) 1304
BseDI CCNNGG 5 cut(s) 370, 541, 870, 1129, 1342
BseGI GGATG 6 cut(s) 261, 391, 461, 857, 914, 1125
BseJI GATNNNNATC 1 cut(s) 1070
BseLI CCNNNNNNNGG 3 cut(s) 211, 630, 1344
BseMI GCAATG 3 cut(s) 87, 127, 600
BseMII CTCAG 5 cut(s) 104, 599, 965, 1219, 1405
BseNI ACTGG 4 cut(s) 307, 624, 1219, 1237
BseXI GCAGC 5 cut(s) 97, 104, 161, 470, 1146
BseYI CCCAGC 1 cut(s) 1278
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 4 cut(s) 215, 792, 845, 1297
BshNI GGYRCC 1 cut(s) 1334
BsiHKAI GWGCWC 2 cut(s) 204, 1402
BsiSI CCGG 1 cut(s) 1129
BslFI GGGAC 2 cut(s) 285, 1169
BslI CCNNNNNNNGG 3 cut(s) 211, 630, 1344
BsmFI GGGAC 2 cut(s) 285, 1169
BsmI GAATGC 2 cut(s) 320, 923
BsnI GGCC 4 cut(s) 215, 792, 845, 1297
Bsp1286I GDGCHC 2 cut(s) 204, 1402
Bsp143I GATC 5 cut(s) 437, 480, 546, 640, 1217
Bsp1720I GCTNAGC 1 cut(s) 113
Bsp19I CCATGG 1 cut(s) 541
BspACI CCGC 2 cut(s) 188, 717
BspANI GGCC 4 cut(s) 215, 792, 845, 1297
BspCNI CTCAG 5 cut(s) 105, 598, 964, 1218, 1404
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 6 cut(s) 439, 548, 855, 934, 1336, 1357
BspPI GGATC 5 cut(s) 432, 445, 541, 554, 1225
BspT107I GGYRCC 1 cut(s) 1334
BsrDI GCAATG 3 cut(s) 87, 127, 600
BsrI ACTGG 4 cut(s) 307, 624, 1219, 1237
BssECI CCNNGG 5 cut(s) 370, 541, 870, 1129, 1342
BssMI GATC 5 cut(s) 437, 480, 546, 640, 1217
BssT1I CCWWGG 4 cut(s) 370, 541, 870, 1342
Bst2UI CCWGG 1 cut(s) 1304
Bst4CI ACNGT 3 cut(s) 276, 535, 1024
Bst6I CTCTTC 1 cut(s) 652
BstC8I GCNNGC 3 cut(s) 90, 712, 980
BstDEI CTNAG 8 cut(s) 113, 295, 570, 585, 951, 974, 1205, 1391
BstDSI CCRYGG 1 cut(s) 541
BstF5I GGATG 6 cut(s) 261, 391, 461, 857, 914, 1125
BstFNI CGCG 1 cut(s) 188
BstKTI GATC 5 cut(s) 440, 483, 549, 643, 1220
BstMBI GATC 5 cut(s) 437, 480, 546, 640, 1217
BstMWI GCNNNNNNNGC 6 cut(s) 98, 162, 171, 716, 1016, 1067
BstNI CCWGG 1 cut(s) 1304
BstNSI RCATGY 4 cut(s) 10, 714, 982, 1440
BstSCI CCNGG 2 cut(s) 1128, 1302
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 5 cut(s) 97, 104, 161, 470, 1146
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 4 cut(s) 437, 480, 546, 640
BstYI RGATCY 4 cut(s) 437, 480, 546, 640
Bsu36I CCTNAGG 1 cut(s) 951
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 4 cut(s) 215, 792, 845, 1297
BtgI CCRYGG 1 cut(s) 541
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 6 cut(s) 261, 391, 461, 857, 914, 1125
BtsIMutI CAGTG 4 cut(s) 540, 711, 1152, 1212
Cac8I GCNNGC 3 cut(s) 90, 712, 980
Cfr13I GGNCC 3 cut(s) 853, 947, 1306
CseI GACGC 1 cut(s) 1126
Csp6I GTAC 2 cut(s) 1169, 1230
CviQI GTAC 2 cut(s) 1169, 1230
DdeI CTNAG 8 cut(s) 113, 295, 570, 585, 951, 974, 1205, 1391
DpnI GATC 5 cut(s) 439, 482, 548, 642, 1219
DpnII GATC 5 cut(s) 437, 480, 546, 640, 1217
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
Ecl136II GAGCTC 1 cut(s) 1400
Eco130I CCWWGG 4 cut(s) 370, 541, 870, 1342
Eco147I AGGCCT 1 cut(s) 1297
Eco24I GRGCYC 1 cut(s) 1402
Eco47I GGWCC 3 cut(s) 853, 947, 1306
Eco53kI GAGCTC 1 cut(s) 1400
Eco57I CTGAAG 2 cut(s) 787, 1430
Eco81I CCTNAGG 1 cut(s) 951
EcoICRI GAGCTC 1 cut(s) 1400
EcoO109I RGGNCCY 1 cut(s) 853
EcoRII CCWGG 1 cut(s) 1302
EcoT14I CCWWGG 4 cut(s) 370, 541, 870, 1342
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 1402
ErhI CCWWGG 4 cut(s) 370, 541, 870, 1342
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 2 cut(s) 285, 1169
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 86, 118, 175, 459, 1135
FokI GGATG 6 cut(s) 268, 378, 448, 844, 901, 1112
FriOI GRGCYC 1 cut(s) 1402
Fsp4HI GCNGC 5 cut(s) 86, 118, 175, 459, 1135
FspBI CTAG 5 cut(s) 455, 920, 1166, 1172, 1343
GluI GCNGC 5 cut(s) 86, 118, 175, 459, 1135
GsaI CCCAGC 1 cut(s) 1282
GsuI CTGGAG 2 cut(s) 152, 746
HaeIII GGCC 4 cut(s) 215, 792, 845, 1297
HapII CCGG 1 cut(s) 1129
HgaI GACGC 1 cut(s) 1126
HindIII AAGCTT 2 cut(s) 163, 886
HinfI GANTC 4 cut(s) 183, 766, 958, 1162
HpaII CCGG 1 cut(s) 1129
HphI GGTGA 3 cut(s) 745, 859, 967
Hpy166II GTNNAC 1 cut(s) 867
Hpy188I TCNGA 1 cut(s) 728
Hpy188III TCNNGA 7 cut(s) 131, 383, 402, 763, 1052, 1110, 1270
Hpy8I GTNNAC 1 cut(s) 867
Hpy99I CGWCG 1 cut(s) 1142
HpyAV CCTTC 6 cut(s) 475, 607, 676, 844, 1100, 1270
HpyCH4III ACNGT 3 cut(s) 276, 535, 1024
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 162, 171, 716, 1016, 1067
HpyF3I CTNAG 8 cut(s) 113, 295, 570, 585, 951, 974, 1205, 1391
Kzo9I GATC 5 cut(s) 437, 480, 546, 640, 1217
LmnI GCTCC 3 cut(s) 72, 932, 1383
Lsp1109I GCAGC 5 cut(s) 97, 104, 161, 470, 1146
LweI GCATC 3 cut(s) 67, 246, 470
MaeI CTAG 5 cut(s) 455, 920, 1166, 1172, 1343
MaeIII GTNAC 4 cut(s) 226, 684, 898, 997
MalI GATC 5 cut(s) 439, 482, 548, 642, 1219
MboI GATC 5 cut(s) 437, 480, 546, 640, 1217
MboII GAAGA 5 cut(s) 306, 475, 639, 793, 1423
MflI RGATCY 4 cut(s) 437, 480, 546, 640
MhlI GDGCHC 2 cut(s) 204, 1402
MluCI AATT 7 cut(s) 17, 220, 450, 737, 1055, 1287, 1323
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 2 cut(s) 574, 1333
Mph1103I ATGCAT 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 1360
MseI TTAA 3 cut(s) 329, 593, 881
MslI CAYNNNNRTG 2 cut(s) 473, 896
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 1 cut(s) 1129
MspR9I CCNGG 2 cut(s) 1130, 1304
Mva1269I GAATGC 2 cut(s) 320, 923
MvaI CCWGG 1 cut(s) 1304
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 6 cut(s) 98, 162, 171, 716, 1016, 1067
NciI CCSGG 1 cut(s) 1130
NcoI CCATGG 1 cut(s) 541
NdeI CATATG 1 cut(s) 289
NdeII GATC 5 cut(s) 437, 480, 546, 640, 1217
NlaIV GGNNCC 6 cut(s) 439, 548, 855, 934, 1336, 1357
NmuCI GTSAC 3 cut(s) 684, 898, 997
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 4 cut(s) 10, 714, 982, 1440
OliI CACNNNNGTG 1 cut(s) 896
PaeI GCATGC 2 cut(s) 714, 982
PceI AGGCCT 1 cut(s) 1297
PciI ACATGT 1 cut(s) 6
PctI GAATGC 2 cut(s) 320, 923
PdmI GAANNNNTTC 1 cut(s) 1360
PfeI GAWTC 3 cut(s) 766, 958, 1162
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 5 cut(s) 87, 119, 176, 460, 1136
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 853
PscI ACATGT 1 cut(s) 6
PshBI ATTAAT 2 cut(s) 329, 881
Psp124BI GAGCTC 1 cut(s) 1402
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 1302
PspFI CCCAGC 1 cut(s) 1278
PspGI CCWGG 1 cut(s) 1302
PspN4I GGNNCC 6 cut(s) 439, 548, 855, 934, 1336, 1357
PspPI GGNCC 3 cut(s) 853, 947, 1306
PspPPI RGGWCCY 1 cut(s) 853
PsuI RGATCY 4 cut(s) 437, 480, 546, 640
PvuII CAGCTG 1 cut(s) 112
RsaI GTAC 2 cut(s) 1170, 1231
RsaNI GTAC 2 cut(s) 1169, 1230
RseI CAYNNNNRTG 2 cut(s) 473, 896
SacI GAGCTC 1 cut(s) 1402
SaqAI TTAA 3 cut(s) 329, 593, 881
SatI GCNGC 5 cut(s) 86, 118, 175, 459, 1135
Sau3AI GATC 5 cut(s) 437, 480, 546, 640, 1217
Sau96I GGNCC 3 cut(s) 853, 947, 1306
ScaI AGTACT 2 cut(s) 1170, 1231
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 1130, 1304
SduI GDGCHC 2 cut(s) 204, 1402
SfaNI GCATC 3 cut(s) 67, 246, 470
SinI GGWCC 3 cut(s) 853, 947, 1306
SmiMI CAYNNNNRTG 2 cut(s) 473, 896
SmlI CTYRAG 2 cut(s) 1079, 1268
SmoI CTYRAG 2 cut(s) 1079, 1268
SphI GCATGC 2 cut(s) 714, 982
Sse9I AATT 7 cut(s) 17, 220, 450, 737, 1055, 1287, 1323
SseBI AGGCCT 1 cut(s) 1297
SsiI CCGC 2 cut(s) 188, 717
SspMI CTAG 5 cut(s) 455, 920, 1166, 1172, 1343
SstI GAGCTC 1 cut(s) 1402
StuI AGGCCT 1 cut(s) 1297
StyD4I CCNGG 2 cut(s) 1128, 1302
StyI CCWWGG 4 cut(s) 370, 541, 870, 1342
TaaI ACNGT 3 cut(s) 276, 535, 1024
TasI AATT 7 cut(s) 17, 220, 450, 737, 1055, 1287, 1323
TatI WGTACW 2 cut(s) 1168, 1229
TfiI GAWTC 3 cut(s) 766, 958, 1162
Tru1I TTAA 3 cut(s) 329, 593, 881
Tru9I TTAA 3 cut(s) 329, 593, 881
TscAI CASTG 4 cut(s) 540, 711, 1152, 1219
TseFI GTSAC 3 cut(s) 684, 898, 997
TseI GCWGC 5 cut(s) 85, 117, 174, 458, 1134
Tsp45I GTSAC 3 cut(s) 684, 898, 997
TspDTI ATGAA 8 cut(s) 17, 457, 475, 589, 758, 863, 916, 1433
TspRI CASTG 4 cut(s) 540, 711, 1152, 1219
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 853, 947, 1306
VspI ATTAAT 2 cut(s) 329, 881
XapI RAATTY 3 cut(s) 450, 1055, 1287
XceI RCATGY 4 cut(s) 10, 714, 982, 1440
XmaJI CCTAGG 1 cut(s) 1342
XmnI GAANNNNTTC 1 cut(s) 1360
XspI CTAG 5 cut(s) 455, 920, 1166, 1172, 1343
ZrmI AGTACT 2 cut(s) 1170, 1231
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.