Rh4DG155200

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014378
Physical Location & Seq
Reverse (-)
30994418 .. 30997215
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG155200.1

Sequence Viewer

Length: 1083 bp
ATGATAGGACTTGGTGTTGTATGTGCTTATTGTTCTCATCTACTCGGGAAATGCCTAGACAAACATCCCAAGTCAAGAAGCTACACAGATATTGGACAGAATGCATTTGGAACCAAAGGAAAACTCCTAGCAGCAACCTTCATTTACTTGGAGATCTTCATGGCCCTTGTGTCCTACACCATCTCATTGCATGACAACATAAACAGAGTTTTCTCGGGGACCCAAGTTCAGATTCCATGGGCCAAGTTATCGAGATCTCAGCTCCTGACGTTGGTGGCGGTTCTGATTGCTCTGCCAAGTCTGTGGTTGAGAGATCTCTCTTCCATATCATTCCTTTCCTTTGGTGGTGTTCTCATGTCAGTTGCCATTTTTTCATCAGTGGCATGCACAGGCATTTTTGGAGGGGTGAAAGCTAATCATAGTATACCAGTCCTTCAGATTCAAAACATTCCTGCAATATCTGGCCTATATATCTTCAGCTATGCAGGACATATTGTCTTTCCTAATTTATACAAGGTCATGAAAGATCCTTCCAAGTTCACCAAGGTATCTATAATAAGCTTCAGCTTGGTCACTCTGCTTTACGGCGCTCTAGCCTTCATGGGTGCCAAGTTTTTTGGTCCCCAAGTAAATCCACAAATCACTCTAAGCATGCCTCCCCATCTTATTGTTACAAAGATTGCACTATGGGCCACAGTGCTCACACCAATGACCAAATATGCTTTGGAATTTGCACCAATGGCAATCCAGCTGGAGCATAATCTCCCAGATTCCATGAGTTCAAGAACAAAGTTGATTGTGAGAGGTACTGTTGGTTCCTTTCTACTTCTGGTGATTCTCGCACTAGCTCTGTCAGTTCCATATTTTCAGTATGTTCTCAGCCTCACTGGATCCCTTGTAAGCATTGCTATTTGTGTCATTTTCCCTTGTGCCTTCTACCTCAAGATCTGTTGGGCTCAGATATCAAGGCCTCTTCTGGTCCTAAACTTCACCTTGATTGCATTTGGGTTTGTTCTTGGAGTGTTTGGAACCATTTCCTCATCAAAGTTGCTCATGCAGAATCTAAGCAGACTCACTGATTAG

Protein Analysis

360

Amino Acids

39.28

Weight (kDa)

9.61

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 1 - 346 3.5e-55 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16740
fragaria_vesca FvH4_4g12300
malus_domestica MD04G1027800.v1.1 MD13G1279400.v1.1
prunus_persica Prupe.1G177200_v2.0.a1
pyrus_communis pycom04g02440 pycom13g29390
rosa_chinensis RchiOBHm_Chr4g0410371
rosa_laevigata RLG00000008434
rosa_multiflora Rmu_sc0002111.1_g000022
rosa_roxburghii Rroxscaffold_5G00355180
rosa_rugosa Rorug04G0102200
rosa_samantha Rh4AG160300 Rh4BG159300 Rh4CG172400 Rh4DG155200
rosa_wichuraiana Rw4G013360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 424
AciI CCGC 1 cut(s) 278
AclWI GGATC 3 cut(s) 521, 885, 898
AcsI RAATTY 1 cut(s) 728
AcuI CTGAAG 3 cut(s) 419, 460, 547
AfaI GTAC 1 cut(s) 808
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 2 cut(s) 443, 783
AhdI GACNNNNNGTC 1 cut(s) 494
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 8 cut(s) 81, 262, 413, 480, 561, 567, 751, 848
AluI AGCT 8 cut(s) 81, 262, 413, 480, 561, 567, 751, 848
Alw21I GWGCWC 1 cut(s) 702
AlwI GGATC 3 cut(s) 521, 885, 898
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 44, 214
AoxI GGCC 5 cut(s) 162, 240, 463, 690, 968
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 1033
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 6 cut(s) 163, 219, 240, 620, 690, 979
AsuHPI GGTGA 4 cut(s) 418, 532, 844, 982
AvaI CYCGRG 2 cut(s) 44, 214
AvaII GGWCC 3 cut(s) 219, 620, 979
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BamHI GGATCC 1 cut(s) 890
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 958
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 1 cut(s) 143
BccI CCATC 2 cut(s) 188, 669
BceAI ACGGC 1 cut(s) 601
BfaI CTAG 4 cut(s) 56, 128, 593, 845
BfoI RGCGCY 1 cut(s) 591
BglII AGATCT 4 cut(s) 153, 254, 313, 945
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme18I GGWCC 3 cut(s) 219, 620, 979
BmeRI GACNNNNNGTC 1 cut(s) 494
BmeT110I CYCGRG 2 cut(s) 44, 214
BmgT120I GGNCC 6 cut(s) 163, 219, 240, 620, 690, 979
BmiI GGNNCC 8 cut(s) 112, 220, 221, 607, 622, 817, 892, 1030
BpmI CTGGAG 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 926
BsaJI CCNNGG 2 cut(s) 236, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 2 cut(s) 428, 892
Bse3DI GCAATG 2 cut(s) 185, 903
BseDI CCNNGG 2 cut(s) 236, 543
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 2 cut(s) 185, 903
BseMII CTCAG 3 cut(s) 272, 892, 971
BseNI ACTGG 2 cut(s) 428, 892
BseXI GCAGC 1 cut(s) 143
BshFI GGCC 5 cut(s) 164, 242, 465, 692, 970
BshNI GGYRCC 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 2 cut(s) 44, 214
BslFI GGGAC 2 cut(s) 232, 606
BslI CCNNNNNNNGG 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 232, 606
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 5 cut(s) 164, 242, 465, 692, 970
BsoBI CYCGRG 2 cut(s) 44, 214
Bsp1286I GDGCHC 2 cut(s) 702, 958
Bsp143I GATC 6 cut(s) 153, 254, 313, 526, 890, 945
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 1 cut(s) 278
BspANI GGCC 5 cut(s) 164, 242, 465, 692, 970
BspCNI CTCAG 3 cut(s) 271, 891, 970
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 8 cut(s) 112, 220, 221, 607, 622, 817, 892, 1030
BspPI GGATC 3 cut(s) 521, 885, 898
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 185, 903
BsrI ACTGG 2 cut(s) 428, 892
BssECI CCNNGG 2 cut(s) 236, 543
BssMI GATC 6 cut(s) 153, 254, 313, 526, 890, 945
BssNAI GTATAC 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 236, 543
Bst1107I GTATAC 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 697, 811
Bst6I CTCTTC 2 cut(s) 325, 978
BstC8I GCNNGC 2 cut(s) 385, 653
BstDEI CTNAG 5 cut(s) 258, 647, 878, 957, 1064
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 6 cut(s) 156, 257, 316, 529, 893, 948
BstMBI GATC 6 cut(s) 153, 254, 313, 526, 890, 945
BstMWI GCNNNNNNNGC 2 cut(s) 689, 740
BstNSI RCATGY 2 cut(s) 387, 655
BstV1I GCAGC 1 cut(s) 143
BstX2I RGATCY 6 cut(s) 153, 254, 313, 526, 890, 945
BstXI CCANNNNNNTGG 1 cut(s) 303
BstYI RGATCY 6 cut(s) 153, 254, 313, 526, 890, 945
BstZ17I GTATAC 1 cut(s) 425
BsuRI GGCC 5 cut(s) 164, 242, 465, 692, 970
BtgI CCRYGG 1 cut(s) 236
BtsCI GGATG 1 cut(s) 64
BtsIMutI CAGTG 4 cut(s) 384, 702, 885, 1074
Cac8I GCNNGC 2 cut(s) 385, 653
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 519
CfoI GCGC 1 cut(s) 590
Cfr13I GGNCC 6 cut(s) 163, 219, 240, 620, 690, 979
Csp6I GTAC 1 cut(s) 807
CviQI GTAC 1 cut(s) 807
DdeI CTNAG 5 cut(s) 258, 647, 878, 957, 1064
DpnI GATC 6 cut(s) 155, 256, 315, 528, 892, 947
DpnII GATC 6 cut(s) 153, 254, 313, 526, 890, 945
DriI GACNNNNNGTC 1 cut(s) 494
Eam1104I CTCTTC 2 cut(s) 325, 978
Eam1105I GACNNNNNGTC 1 cut(s) 494
EarI CTCTTC 2 cut(s) 325, 978
Eco130I CCWWGG 2 cut(s) 236, 543
Eco147I AGGCCT 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 958
Eco32I GATATC 1 cut(s) 963
Eco47I GGWCC 3 cut(s) 219, 620, 979
Eco57I CTGAAG 3 cut(s) 419, 460, 547
Eco88I CYCGRG 2 cut(s) 44, 214
EcoO109I RGGNCCY 1 cut(s) 219
EcoRV GATATC 1 cut(s) 963
EcoT14I CCWWGG 2 cut(s) 236, 543
EcoT22I ATGCAT 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 2 cut(s) 236, 543
FaqI GGGAC 2 cut(s) 232, 606
FblI GTMKAC 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 51
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 4 cut(s) 56, 128, 593, 845
GlaI GCGC 1 cut(s) 589
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 773
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 5 cut(s) 164, 242, 465, 692, 970
HhaI GCGC 1 cut(s) 590
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 6 cut(s) 232, 439, 770, 835, 1060, 1071
HphI GGTGA 4 cut(s) 418, 532, 844, 982
Hpy166II GTNNAC 2 cut(s) 425, 540
Hpy188I TCNGA 4 cut(s) 231, 285, 438, 960
Hpy188III TCNNGA 7 cut(s) 46, 75, 252, 265, 520, 783, 943
Hpy8I GTNNAC 2 cut(s) 425, 540
HpyAV CCTTC 5 cut(s) 148, 443, 540, 607, 943
HpyCH4III ACNGT 2 cut(s) 697, 811
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 9 cut(s) 104, 190, 387, 455, 485, 683, 734, 1001, 1057
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 740
HpyF3I CTNAG 5 cut(s) 258, 647, 878, 957, 1064
HpySE526I ACGT 1 cut(s) 269
HspAI GCGC 1 cut(s) 588
KflI GGGWCCC 1 cut(s) 219
Kzo9I GATC 6 cut(s) 153, 254, 313, 526, 890, 945
LmnI GCTCC 2 cut(s) 267, 754
Lsp1109I GCAGC 1 cut(s) 143
MaeI CTAG 4 cut(s) 56, 128, 593, 845
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 2 cut(s) 571, 670
MalI GATC 6 cut(s) 155, 256, 315, 528, 892, 947
MboI GATC 6 cut(s) 153, 254, 313, 526, 890, 945
MboII GAAGA 4 cut(s) 148, 312, 466, 965
MflI RGATCY 6 cut(s) 153, 254, 313, 526, 890, 945
MhlI GDGCHC 2 cut(s) 702, 958
MluCI AATT 2 cut(s) 505, 728
MlyI GAGTC 1 cut(s) 1065
MnlI CCTC 7 cut(s) 395, 666, 797, 893, 950, 981, 1048
Mph1103I ATGCAT 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 1033
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 751
Mva1269I GAATGC 1 cut(s) 106
MwoI GCNNNNNNNGC 2 cut(s) 689, 740
NcoI CCATGG 1 cut(s) 236
NdeII GATC 6 cut(s) 153, 254, 313, 526, 890, 945
NlaIV GGNNCC 8 cut(s) 112, 220, 221, 607, 622, 817, 892, 1030
NmuCI GTSAC 1 cut(s) 571
NsiI ATGCAT 1 cut(s) 106
NspI RCATGY 2 cut(s) 387, 655
PaeI GCATGC 2 cut(s) 387, 655
PagI TCATGA 1 cut(s) 519
PceI AGGCCT 1 cut(s) 970
PctI GAATGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 5 cut(s) 232, 439, 770, 835, 1060
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 1065
PpsI GAGTC 1 cut(s) 1065
PpuMI RGGWCCY 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 219
PspN4I GGNNCC 8 cut(s) 112, 220, 221, 607, 622, 817, 892, 1030
PspPI GGNCC 6 cut(s) 163, 219, 240, 620, 690, 979
PspPPI RGGWCCY 1 cut(s) 219
PsrI GAACNNNNNNTAC 2 cut(s) 799, 831
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 6 cut(s) 153, 254, 313, 526, 890, 945
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 1 cut(s) 808
RsaNI GTAC 1 cut(s) 807
RseI CAYNNNNRTG 1 cut(s) 707
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 6 cut(s) 153, 254, 313, 526, 890, 945
Sau96I GGNCC 6 cut(s) 163, 219, 240, 620, 690, 979
SchI GAGTC 1 cut(s) 1065
SduI GDGCHC 2 cut(s) 702, 958
SinI GGWCC 3 cut(s) 219, 620, 979
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SphI GCATGC 2 cut(s) 387, 655
Sse9I AATT 2 cut(s) 505, 728
SseBI AGGCCT 1 cut(s) 970
SsiI CCGC 1 cut(s) 278
SspMI CTAG 4 cut(s) 56, 128, 593, 845
StuI AGGCCT 1 cut(s) 970
StyI CCWWGG 2 cut(s) 236, 543
TaaI ACNGT 2 cut(s) 697, 811
TaiI ACGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 251
TasI AATT 2 cut(s) 505, 728
TfiI GAWTC 5 cut(s) 232, 439, 770, 835, 1060
TscAI CASTG 4 cut(s) 384, 702, 892, 1081
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 5 cut(s) 130, 148, 363, 536, 589
TspRI CASTG 4 cut(s) 384, 702, 892, 1081
VpaK11BI GGWCC 3 cut(s) 219, 620, 979
XapI RAATTY 1 cut(s) 728
XceI RCATGY 2 cut(s) 387, 655
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 4 cut(s) 56, 128, 593, 845
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.