AT5G18660

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013241
Physical Location & Seq
Reverse (-)
6220710 .. 6222300
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18660.1

Sequence Viewer

Length: 1254 bp
ATGTCACTTTGCTCTTCCTTCAACGTATTCGCTTCCTACTCACCAAAACCCAAAACCATCTTCAAAGATTCCAAATTCATTTCACAATTTCAGGTAAAGTCGTCTCCTTTAGCTTCAACATTCCACACAAATGAATCCTCAACGAGTCTTAAGTATAAGAGAGCGAGACTCAAACCAATTTCATCTCTAGACTCGGGAATTTCAGAAATCGCTACATCACCTTCATTCAGGAACAAAAGCCCTAAAGATATAAACGTTTTAGTGGTTGGTTCAACTGGGTACATAGGTAGATTTGTAGTGAAAGAGATGATCAAAAGAGGGTTCAATGTGATTGCTGTTGCGAGAGAGAAGAGTGGGATTAGAGGCAAAAACGACAAGGAAGAGACTTTGAAGCAATTACAAGGCGCCAATGTGTGTTTCTCTGATGTTACTGAGTTAGATGTTCTTGAGAAATCTATTGAGAATCTAGGATTTGGTGTTGATGTTGTTGTTTCCTGTCTCGCTAGTCGTAATGGAGGGATTAAAGATTCGTGGAAGATTGATTATGAAGCTACCAAAAACAGTCTTGTAGCTGGGAAGAAGTTTGGTGCTAAGCATTTTGTTTTGCTCTCTGCGATTTGTGTGCAGAAGCCTTTACTGGAGTTTCAGAGAGCGAAACTGAAGTTCGAAGCAGAGTTGATGGATTTGGCTGAGCAACAAGACTCTAGCTTTACATACAGCATTGTGAGACCAACGGCTTTTTTCAAGAGCTTAGGTGGTCAAGTTGAGATTGTGAAAGATGGGAAACCTTACGTGATGTTTGGAGATGGTAAGCTATGCGCTTGTAAACCGATCAGCGAACAAGATTTAGCAGCGTTTATAGCGGATTGCGTGTTGGAAGAGAACAAGATCAATCAAGTTTTGCCAATTGGTGGACCAGGCAAGGCATTAACGCCATTAGAGCAAGGAGAGATTCTGTTTAAGATTCTTGGGAGAGAGCCTAAGTTCTTGAAAGTACCTATCGAGATTATGGATTTTGTGATTGGTGTTCTCGATTCTATTGCAAAGATCTTTCCTTCGGTTGGAGAGGCTGCCGAATTTGGGAAGATTGGAAGGTATTACGCCGCAGAGAGTATGTTGATTCTTGATCCAGAGACTGGAGAGTATAGCGAGGAGAAGACACCGAGTTACGGGAAAGACACGCTTGAGGATTTCTTTGCGAAAGTGATCAGAGAAGGCATGGCGGGTCAAGAACTCGGTGAACAGTTCTTCTAG

Protein Analysis

417

Amino Acids

45.89

Weight (kDa)

7.47

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 85 - 375 1.9e-18 NmrA-like family
Epimerase PF01370 86 - 208 1.1e-10 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 87 - 208 2.4e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 90 - 293 4.7e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013241)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18660
fragaria_vesca FvH4_4g17240
malus_domestica MD13G1173600.v1.1
prunus_persica Prupe.1G141800_v2.0.a1
pyrus_communis pycom16g14670
rosa_chinensis RchiOBHm_Chr4g0421151
rosa_laevigata RLG00000007711
rosa_multiflora Rmu_co8498633.1_g000001 Rmu_sc0009290.1_g000003
rosa_roxburghii Rroxscaffold_5G00363440
rosa_rugosa Rorug04G0170000 Rorug04G0170100 Rorug04G0170200
rosa_samantha Rh4AG229600 Rh4BG232300 Rh4CG243900 Rh4DG228000
rosa_wichuraiana Rw4G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 2 cut(s) 911, 1136
AciI CCGC 3 cut(s) 863, 1104, 1223
AclI AACGTT 1 cut(s) 255
AclWI GGATC 1 cut(s) 1121
AcsI RAATTY 3 cut(s) 74, 198, 1076
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 2 cut(s) 281, 996
AfiI CCNNNNNNNGG 5 cut(s) 911, 1061, 1080, 1136, 1169
AflII CTTAAG 1 cut(s) 149
AgsI TTSAA 8 cut(s) 22, 64, 117, 273, 325, 391, 745, 991
AjnI CCWGG 1 cut(s) 916
AjuI GAANNNNNNNTTGG 4 cut(s) 44, 76, 401, 433
AluBI AGCT 6 cut(s) 113, 551, 572, 708, 750, 814
AluI AGCT 6 cut(s) 113, 551, 572, 708, 750, 814
Alw26I GTCTC 6 cut(s) 108, 160, 377, 503, 721, 1127
AlwI GGATC 1 cut(s) 1121
AlwNI CAGNNNCTG 1 cut(s) 1136
Ama87I CYCGRG 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 851, 1070
ApoI RAATTY 3 cut(s) 74, 198, 1076
AspLEI GCGC 2 cut(s) 407, 821
AspS9I GGNCC 1 cut(s) 914
AsuHPI GGTGA 3 cut(s) 33, 210, 1250
AsuII TTCGAA 1 cut(s) 666
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 1 cut(s) 914
BaeI ACNNNNGTAYC 2 cut(s) 271, 304
BanI GGYRCC 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 1163
BbvI GCAGC 2 cut(s) 863, 1057
BccI CCATC 4 cut(s) 65, 673, 773, 800
BceAI ACGGC 1 cut(s) 750
BcgI CGANNNNNNTGC 4 cut(s) 604, 638, 1022, 1056
BciT130I CCWGG 1 cut(s) 918
BclI TGATCA 2 cut(s) 309, 1206
BcoDI GTCTC 6 cut(s) 108, 160, 377, 503, 721, 1127
BfaI CTAG 5 cut(s) 188, 467, 504, 705, 1252
BfoI RGCGCY 1 cut(s) 408
BfrI CTTAAG 1 cut(s) 149
BglII AGATCT 1 cut(s) 1047
BisI GCNGC 3 cut(s) 852, 1071, 1104
BlpI GCTNAGC 2 cut(s) 591, 690
BlsI GCNGC 3 cut(s) 853, 1072, 1105
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 1 cut(s) 914
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 914
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 918
BmrI ACTGGG 1 cut(s) 285
BmuI ACTGGG 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 1163
BplI GAGNNNNNCTC 2 cut(s) 153, 185
BpmI CTGGAG 2 cut(s) 659, 1158
Bpu10I CCTNAGC 1 cut(s) 751
Bpu1102I GCTNAGC 2 cut(s) 591, 690
Bpu14I TTCGAA 1 cut(s) 666
BpuEI CTTGAG 2 cut(s) 467, 1205
BsaAI YACGTR 1 cut(s) 793
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 721
Bsc4I CCNNNNNNNGG 5 cut(s) 911, 1061, 1080, 1136, 1169
Bse1I ACTGG 3 cut(s) 280, 642, 1141
BseBI CCWGG 1 cut(s) 918
BseLI CCNNNNNNNGG 5 cut(s) 911, 1061, 1080, 1136, 1169
BseMII CTCAG 2 cut(s) 423, 681
BseNI ACTGG 3 cut(s) 280, 642, 1141
BseRI GAGGAG 1 cut(s) 1166
BseXI GCAGC 2 cut(s) 863, 1057
BseYI CCCAGC 1 cut(s) 572
BsgI GTGCAG 1 cut(s) 644
BshNI GGYRCC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 193
BslI CCNNNNNNNGG 5 cut(s) 911, 1061, 1080, 1136, 1169
BsmAI GTCTC 6 cut(s) 108, 160, 377, 503, 721, 1127
BsmBI CGTCTC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 721
BsoBI CYCGRG 1 cut(s) 193
Bsp119I TTCGAA 1 cut(s) 666
Bsp143I GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
Bsp1720I GCTNAGC 2 cut(s) 591, 690
BspACI CCGC 3 cut(s) 863, 1104, 1223
BspCNI CTCAG 2 cut(s) 424, 682
BspLI GGNNCC 1 cut(s) 406
BspPI GGATC 1 cut(s) 1121
BspQI GCTCTTC 1 cut(s) 19
BspT104I TTCGAA 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 404
BspTI CTTAAG 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 721
BsrI ACTGG 3 cut(s) 280, 642, 1141
BssMI GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
BssNI GRCGYC 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 2 cut(s) 563, 1245
Bst6I CTCTTC 4 cut(s) 19, 344, 375, 873
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 666
BstDEI CTNAG 5 cut(s) 432, 591, 690, 751, 981
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 2 cut(s) 407, 821
BstKTI GATC 6 cut(s) 312, 834, 891, 1050, 1129, 1209
BstMAI GTCTC 6 cut(s) 108, 160, 377, 503, 721, 1127
BstMBI GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
BstMWI GCNNNNNNNGC 2 cut(s) 860, 940
BstNI CCWGG 1 cut(s) 918
BstSCI CCNGG 1 cut(s) 916
BstV1I GCAGC 2 cut(s) 863, 1057
BstV2I GAAGAC 1 cut(s) 1163
BstX2I RGATCY 1 cut(s) 1047
BstYI RGATCY 1 cut(s) 1047
CaiI CAGNNNCTG 1 cut(s) 1136
CfoI GCGC 2 cut(s) 407, 821
Cfr13I GGNCC 1 cut(s) 914
Csp6I GTAC 2 cut(s) 280, 995
CviAII CATG 1 cut(s) 1219
CviQI GTAC 2 cut(s) 280, 995
DdeI CTNAG 5 cut(s) 432, 591, 690, 751, 981
DinI GGCGCC 1 cut(s) 406
DpnI GATC 6 cut(s) 311, 833, 890, 1049, 1128, 1208
DpnII GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
Eam1104I CTCTTC 4 cut(s) 19, 344, 375, 873
EarI CTCTTC 4 cut(s) 19, 344, 375, 873
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 914
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 916
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 1 cut(s) 1222
FalI AAGNNNNNCTT 2 cut(s) 1167, 1199
FatI CATG 1 cut(s) 1218
FauI CCCGC 1 cut(s) 1216
FbaI TGATCA 2 cut(s) 309, 1206
Fnu4HI GCNGC 3 cut(s) 852, 1071, 1104
Fsp4HI GCNGC 3 cut(s) 852, 1071, 1104
FspBI CTAG 5 cut(s) 188, 467, 504, 705, 1252
GlaI GCGC 2 cut(s) 406, 820
GluI GCNGC 3 cut(s) 852, 1071, 1104
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 2 cut(s) 659, 1158
HaeII RGCGCY 1 cut(s) 408
HhaI GCGC 2 cut(s) 407, 821
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 1 cut(s) 1222
Hin6I GCGC 2 cut(s) 405, 819
HinP1I GCGC 2 cut(s) 405, 819
HphI GGTGA 3 cut(s) 33, 210, 1250
Hpy166II GTNNAC 3 cut(s) 827, 914, 1241
Hpy188I TCNGA 4 cut(s) 205, 424, 648, 1211
Hpy8I GTNNAC 3 cut(s) 827, 914, 1241
HpyAV CCTTC 5 cut(s) 28, 231, 1065, 1086, 1208
HpyCH4III ACNGT 2 cut(s) 563, 1245
HpyCH4IV ACGT 3 cut(s) 24, 255, 792
HpyCH4V TGCA 2 cut(s) 625, 1043
HpyF10VI GCNNNNNNNGC 2 cut(s) 860, 940
HpyF3I CTNAG 5 cut(s) 432, 591, 690, 751, 981
HpySE526I ACGT 3 cut(s) 24, 255, 792
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 1 cut(s) 1222
HspAI GCGC 2 cut(s) 405, 819
KasI GGCGCC 1 cut(s) 404
Ksp22I TGATCA 2 cut(s) 309, 1206
Kzo9I GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
LguI GCTCTTC 1 cut(s) 19
Lsp1109I GCAGC 2 cut(s) 863, 1057
MaeI CTAG 5 cut(s) 188, 467, 504, 705, 1252
MaeII ACGT 3 cut(s) 24, 255, 792
MaeIII GTNAC 3 cut(s) 3, 427, 1166
MalI GATC 6 cut(s) 311, 833, 890, 1049, 1128, 1208
MboI GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 1047
MluCI AATT 7 cut(s) 74, 86, 177, 198, 395, 906, 1076
Mly113I GGCGCC 1 cut(s) 405
MlyI GAGTC 4 cut(s) 154, 162, 185, 695
MmeI TCCRAC 2 cut(s) 855, 1042
MnlI CCTC 7 cut(s) 148, 311, 356, 509, 1060, 1144, 1180
MseI TTAA 4 cut(s) 150, 522, 929, 960
MslI CAYNNNNRTG 1 cut(s) 129
MspCI CTTAAG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 918
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 1 cut(s) 918
MwoI GCNNNNNNNGC 2 cut(s) 860, 940
NarI GGCGCC 1 cut(s) 405
NdeII GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
NlaIII CATG 1 cut(s) 1222
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 19
PfeI GAWTC 8 cut(s) 68, 134, 463, 527, 952, 964, 1034, 1120
PflMI CCANNNNNTGG 2 cut(s) 911, 1136
PkrI GCNGC 3 cut(s) 853, 1072, 1105
PleI GAGTC 4 cut(s) 153, 162, 185, 695
PluTI GGCGCC 1 cut(s) 408
PpsI GAGTC 4 cut(s) 153, 162, 185, 695
Ppu21I YACGTR 1 cut(s) 793
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 916
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 914
PstNI CAGNNNCTG 1 cut(s) 1136
PsuI RGATCY 1 cut(s) 1047
RsaI GTAC 2 cut(s) 281, 996
RsaNI GTAC 2 cut(s) 280, 995
RseI CAYNNNNRTG 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 4 cut(s) 150, 522, 929, 960
SatI GCNGC 3 cut(s) 852, 1071, 1104
Sau3AI GATC 6 cut(s) 309, 831, 888, 1047, 1126, 1206
Sau96I GGNCC 1 cut(s) 914
SchI GAGTC 4 cut(s) 154, 162, 185, 695
ScrFI CCNGG 1 cut(s) 918
SfoI GGCGCC 1 cut(s) 406
SfuI TTCGAA 1 cut(s) 666
SinI GGWCC 1 cut(s) 914
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 3 cut(s) 149, 446, 1184
SmoI CTYRAG 3 cut(s) 149, 446, 1184
Sse9I AATT 7 cut(s) 74, 86, 177, 198, 395, 906, 1076
SsiI CCGC 3 cut(s) 863, 1104, 1223
SspDI GGCGCC 1 cut(s) 404
SspMI CTAG 5 cut(s) 188, 467, 504, 705, 1252
StyD4I CCNGG 1 cut(s) 916
TaaI ACNGT 2 cut(s) 563, 1245
TaiI ACGT 3 cut(s) 27, 258, 795
TaqI TCGA 3 cut(s) 666, 1002, 1032
TasI AATT 7 cut(s) 74, 86, 177, 198, 395, 906, 1076
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 8 cut(s) 68, 134, 463, 527, 952, 964, 1034, 1120
Tru1I TTAA 4 cut(s) 150, 522, 929, 960
Tru9I TTAA 4 cut(s) 150, 522, 929, 960
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 851, 1070
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 5 cut(s) 67, 147, 171, 213, 561
Van91I CCANNNNNTGG 2 cut(s) 911, 1136
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 914
XapI RAATTY 3 cut(s) 74, 198, 1076
XbaI TCTAGA 1 cut(s) 187
XspI CTAG 5 cut(s) 188, 467, 504, 705, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.