RchiOBHm_Chr4g0421151

Divinyl chlorophyllide a 8-vinyl-reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013241
Physical Location & Seq
Forward (+)
46824180 .. 46825837
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39081

Sequence Viewer

Length: 1245 bp
ATGTCACTGTGCTTCTCCTCCACCGCATTCAATCTTCACTCAGCAAAAAACCAGAGCTTCTTAACGCGTTTCTCTTCTCACTTCACCAACCCAACTAAGGTAACCTCTTCTCTCTTTACGGGGTTCCCATCATCTCCGTTAAGCTTATCTGAAGTCGTTAAGTTCAGCAAAGACAGACTAAAACCCATTTCGGCTTCAACGACCGTTACACCTTCATTCAGGAACAAAAGTCCCGAAGACATTAACGTTTTGGTTGTGGGTGCAACTGGGTACATTGGAAAGTTTGTGGTTAAGGAGTTAGTGGATAGAGAGTTCAATGTTATAGCTATTGCTAGGGAGAAGAGTGGGATTAGAGGTAAGTTTAGCAAGGATGAGACTTTAAACCAGTTGAAAGGAGCCAATGTGTGCTTTTCAGATGTGACCAATTTGGATAGTTTGGAGAAATCTATGGAAGAATTGGGTGTTTCAATTGATGTAGTGGTTTCTTGCCTTGCTAGCCGTAGTGGTGGTGTGAAGGATTCTTGGAAGATAGACTATGAGGCAACAAAGAACAGTCTTGTTGCCGGTAGGAATCGTGGGGCTTCACATTTTGTGTTGCTTTCTGCAATATGTGTGCAGAAGCCTCTTCTTGAATTCCAGCGCGCAAAGCTGAAGTTCGAGGATGAGTTGATCAAGGAAGCTAAGGAGGACAGTGGGTTCAGTTATAGTATTGTGAGGCCAACTGCATTCTTTAAGAGCTTGGGAGGTCAGGTTGAGTTGGTGAAAGATGGGAAGCCGTATGTGATGTTTGGGGATGGGAAGTTGTGTGCTTGCAAGCCGATTAGTGAGGCGGATTTGGCTTCTTTTATTGCTGATTGTGTGTTGAGTGAAAATAAGATTAACCAGGTTTTGCCTATTGGTGGACCGGGGAAGGCATTGACACCAATGGAGCAAGGGGAGTTACTGTTTAGACTTGTGGGGAGGGAACCCAAGTTCTTGAAAGTGCCGATTGAGATAATGGACTTTGCTATTGGGGTACTTGATTTCCTTGTAAAGATATTTCCTTCAATGGAAGACACAGCCGAGTTCGGAAAGATTGGAAGGTATTATGCTGCTGAGAGTATGTTGGTTTTGGATCCTGAGACCGGAGAATATAGTGCTGAGAAAACACCAAGTTATGGCAAAGACACATTGGAAGACTTCTTCCAGACTGTGCTGAGAGAGGGGATGGCTGGCCAGGAATTGGGGGAGCAAACAATTTTCTGA

Protein Analysis

414

Amino Acids

45.44

Weight (kDa)

5.71

Isoelectric Point (pI)

23.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 82 - 371 6.4e-13 NmrA-like family
Epimerase PF01370 83 - 205 9.1e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 84 - 205 1.1e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 87 - 288 5.5e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013241)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18660
fragaria_vesca FvH4_4g17240
malus_domestica MD13G1173600.v1.1
prunus_persica Prupe.1G141800_v2.0.a1
pyrus_communis pycom16g14670
rosa_chinensis RchiOBHm_Chr4g0421151
rosa_laevigata RLG00000007711
rosa_multiflora Rmu_co8498633.1_g000001 Rmu_sc0009290.1_g000003
rosa_roxburghii Rroxscaffold_5G00363440
rosa_rugosa Rorug04G0170000 Rorug04G0170100 Rorug04G0170200
rosa_samantha Rh4AG229600 Rh4BG232300 Rh4CG243900 Rh4DG228000
rosa_wichuraiana Rw4G020420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1157, 1222
AccII CGCG 2 cut(s) 67, 642
AciI CCGC 2 cut(s) 24, 830
AclI AACGTT 1 cut(s) 246
AclWI GGATC 2 cut(s) 1109, 1122
AcoI YGGCCR 1 cut(s) 1213
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 2 cut(s) 171, 671
AfaI GTAC 2 cut(s) 272, 1017
AfiI CCNNNNNNNGG 5 cut(s) 97, 899, 1124, 1157, 1222
AflIII ACRYGT 1 cut(s) 65
AgsI TTSAA 8 cut(s) 31, 198, 316, 391, 468, 632, 979, 1047
AjnI CCWGG 2 cut(s) 882, 1215
AloI GAACNNNNNNTCC 6 cut(s) 296, 328, 680, 712, 956, 988
AluBI AGCT 6 cut(s) 57, 144, 326, 649, 680, 738
AluI AGCT 6 cut(s) 57, 144, 326, 649, 680, 738
Alw26I GTCTC 2 cut(s) 368, 1115
AlwI GGATC 2 cut(s) 1109, 1122
AoxI GGCC 2 cut(s) 716, 1213
ApeKI GCWGC 1 cut(s) 1091
ApoI RAATTY 1 cut(s) 632
AspLEI GCGC 2 cut(s) 642, 644
AspS9I GGNCC 1 cut(s) 902
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 2 cut(s) 76, 772
AsuNHI GCTAGC 1 cut(s) 494
AvaII GGWCC 1 cut(s) 902
BalI TGGCCA 1 cut(s) 1215
BamHI GGATCC 1 cut(s) 1114
BbsI GAAGAC 3 cut(s) 243, 1059, 1182
BbvI GCAGC 1 cut(s) 1078
BccI CCATC 4 cut(s) 136, 761, 788, 1201
BceAI ACGGC 2 cut(s) 483, 760
BciT130I CCWGG 2 cut(s) 884, 1217
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 2 cut(s) 368, 1115
BfaI CTAG 2 cut(s) 333, 495
BisI GCNGC 1 cut(s) 1092
BlsI GCNGC 1 cut(s) 1093
Bme1390I CCNGG 3 cut(s) 884, 906, 1217
Bme18I GGWCC 1 cut(s) 902
BmgT120I GGNCC 1 cut(s) 902
BmiI GGNNCC 4 cut(s) 125, 397, 966, 1116
BmrFI CCNGG 3 cut(s) 884, 906, 1217
BmrI ACTGGG 1 cut(s) 276
BmtI GCTAGC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 276
BpiI GAAGAC 3 cut(s) 243, 1059, 1182
Bpu10I CCTNAGC 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 906
BsaI GGTCTC 1 cut(s) 1115
BsaJI CCNNGG 1 cut(s) 905
BsaWI WCCGGW 1 cut(s) 1124
BsaXI ACNNNNNCTCC 6 cut(s) 387, 417, 735, 765, 929, 959
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 899, 1124, 1157, 1222
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 271, 385
BseBI CCWGG 2 cut(s) 884, 1217
BseDI CCNNGG 1 cut(s) 905
BseGI GGATG 4 cut(s) 376, 667, 799, 1212
BseLI CCNNNNNNNGG 5 cut(s) 97, 899, 1124, 1157, 1222
BseMII CTCAG 5 cut(s) 54, 1086, 1110, 1131, 1187
BseNI ACTGG 2 cut(s) 271, 385
BsePI GCGCGC 1 cut(s) 640
BseRI GAGGAG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 1078
BsgI GTGCAG 1 cut(s) 635
Bsh1236I CGCG 2 cut(s) 67, 642
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 718, 1215
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 564, 905, 1125
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 5 cut(s) 97, 899, 1124, 1157, 1222
BsmAI GTCTC 2 cut(s) 368, 1115
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 2 cut(s) 26, 725
BsnI GGCC 2 cut(s) 718, 1215
Bso31I GGTCTC 1 cut(s) 1115
Bsp143I GATC 2 cut(s) 669, 1114
BspACI CCGC 2 cut(s) 24, 830
BspANI GGCC 2 cut(s) 718, 1215
BspCNI CTCAG 5 cut(s) 53, 1087, 1111, 1132, 1188
BspFNI CGCG 2 cut(s) 67, 642
BspLI GGNNCC 4 cut(s) 125, 397, 966, 1116
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 1109, 1122
BspTNI GGTCTC 1 cut(s) 1115
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 2 cut(s) 271, 385
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 905
BssHII GCGCGC 1 cut(s) 640
BssMI GATC 2 cut(s) 669, 1114
Bst2UI CCWGG 2 cut(s) 884, 1217
Bst4CI ACNGT 6 cut(s) 9, 205, 554, 692, 945, 1192
Bst6I CTCTTC 4 cut(s) 79, 112, 335, 630
BstC8I GCNNGC 5 cut(s) 496, 642, 811, 815, 1213
BstDEI CTNAG 7 cut(s) 40, 96, 681, 1095, 1119, 1140, 1196
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 4 cut(s) 376, 667, 799, 1212
BstFNI CGCG 2 cut(s) 67, 642
BstHHI GCGC 2 cut(s) 642, 644
BstKTI GATC 2 cut(s) 672, 1117
BstMAI GTCTC 2 cut(s) 368, 1115
BstMBI GATC 2 cut(s) 669, 1114
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 495, 646, 836
BstNI CCWGG 2 cut(s) 884, 1217
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 882, 904, 1215
BstUI CGCG 2 cut(s) 67, 642
BstV1I GCAGC 1 cut(s) 1078
BstV2I GAAGAC 3 cut(s) 243, 1059, 1182
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
BsuRI GGCC 2 cut(s) 718, 1215
BtsCI GGATG 4 cut(s) 376, 667, 799, 1212
BtsIMutI CAGTG 2 cut(s) 5, 697
Cac8I GCNNGC 5 cut(s) 496, 642, 811, 815, 1213
CfoI GCGC 2 cut(s) 642, 644
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 1 cut(s) 902
CsiI ACCWGGT 1 cut(s) 882
Csp6I GTAC 2 cut(s) 271, 1016
CviQI GTAC 2 cut(s) 271, 1016
DdeI CTNAG 7 cut(s) 40, 96, 681, 1095, 1119, 1140, 1196
DpnI GATC 2 cut(s) 671, 1116
DpnII GATC 2 cut(s) 669, 1114
DraI TTTAAA 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 1213
Eam1104I CTCTTC 4 cut(s) 79, 112, 335, 630
EarI CTCTTC 4 cut(s) 79, 112, 335, 630
EciI GGCGGA 1 cut(s) 845
Eco31I GGTCTC 1 cut(s) 1115
Eco47I GGWCC 1 cut(s) 902
Eco57I CTGAAG 2 cut(s) 171, 671
Eco91I GGTNACC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 882, 1215
FaqI GGGAC 1 cut(s) 216
FbaI TGATCA 1 cut(s) 669
Fnu4HI GCNGC 1 cut(s) 1092
FokI GGATG 4 cut(s) 383, 674, 806, 1219
Fsp4HI GCNGC 1 cut(s) 1092
FspBI CTAG 2 cut(s) 333, 495
GlaI GCGC 2 cut(s) 641, 643
GluI GCNGC 1 cut(s) 1092
HaeIII GGCC 2 cut(s) 718, 1215
HapII CCGG 3 cut(s) 564, 905, 1125
HhaI GCGC 2 cut(s) 642, 644
Hin6I GCGC 2 cut(s) 640, 642
HinP1I GCGC 2 cut(s) 640, 642
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 2 cut(s) 518, 571
HpaII CCGG 3 cut(s) 564, 905, 1125
HphI GGTGA 2 cut(s) 76, 772
Hpy166II GTNNAC 1 cut(s) 902
Hpy188I TCNGA 4 cut(s) 151, 415, 1070, 1244
Hpy188III TCNNGA 6 cut(s) 220, 233, 629, 976, 1118, 1186
Hpy8I GTNNAC 1 cut(s) 902
HpyAV CCTTC 5 cut(s) 222, 508, 904, 1053, 1074
HpyCH4III ACNGT 6 cut(s) 9, 205, 554, 692, 945, 1192
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 5 cut(s) 263, 605, 616, 725, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 495, 646, 836
HpyF3I CTNAG 7 cut(s) 40, 96, 681, 1095, 1119, 1140, 1196
HpySE526I ACGT 1 cut(s) 246
HspAI GCGC 2 cut(s) 640, 642
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 2 cut(s) 669, 1114
LmnI GCTCC 3 cut(s) 395, 928, 1228
Lsp1109I GCAGC 1 cut(s) 1078
MabI ACCWGGT 1 cut(s) 882
MaeI CTAG 2 cut(s) 333, 495
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 5 cut(s) 3, 100, 205, 418, 939
MalI GATC 2 cut(s) 671, 1116
MboI GATC 2 cut(s) 669, 1114
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 1 cut(s) 1114
MlsI TGGCCA 1 cut(s) 1215
MluCI AATT 6 cut(s) 424, 455, 468, 632, 1220, 1236
MluI ACGCGT 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 1215
Mox20I TGGCCA 1 cut(s) 1215
MscI TGGCCA 1 cut(s) 1215
MseI TTAA 8 cut(s) 62, 140, 159, 243, 291, 380, 732, 879
Msp20I TGGCCA 1 cut(s) 1215
MspI CCGG 3 cut(s) 564, 905, 1125
MspR9I CCNGG 3 cut(s) 884, 906, 1217
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 2 cut(s) 26, 725
MvaI CCWGG 2 cut(s) 884, 1217
MvnI CGCG 2 cut(s) 67, 642
MwoI GCNNNNNNNGC 3 cut(s) 495, 646, 836
NciI CCSGG 1 cut(s) 906
NdeII GATC 2 cut(s) 669, 1114
NheI GCTAGC 1 cut(s) 494
NlaIV GGNNCC 4 cut(s) 125, 397, 966, 1116
NmeAIII GCCGAG 1 cut(s) 1087
NmuCI GTSAC 2 cut(s) 3, 418
PauI GCGCGC 1 cut(s) 640
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PctI GAATGC 2 cut(s) 26, 725
PfeI GAWTC 2 cut(s) 518, 571
PflMI CCANNNNNTGG 2 cut(s) 1157, 1222
PkrI GCNGC 1 cut(s) 1093
Psp1406I AACGTT 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 882, 1215
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 2 cut(s) 882, 1215
PspN4I GGNNCC 4 cut(s) 125, 397, 966, 1116
PspPI GGNCC 1 cut(s) 902
PsuI RGATCY 1 cut(s) 1114
PteI GCGCGC 1 cut(s) 640
RsaI GTAC 2 cut(s) 272, 1017
RsaNI GTAC 2 cut(s) 271, 1016
SaqAI TTAA 8 cut(s) 62, 140, 159, 243, 291, 380, 732, 879
SatI GCNGC 1 cut(s) 1092
Sau3AI GATC 2 cut(s) 669, 1114
Sau96I GGNCC 1 cut(s) 902
ScrFI CCNGG 3 cut(s) 884, 906, 1217
SexAI ACCWGGT 1 cut(s) 882
SinI GGWCC 1 cut(s) 902
Sse9I AATT 6 cut(s) 424, 455, 468, 632, 1220, 1236
SsiI CCGC 2 cut(s) 24, 830
SspMI CTAG 2 cut(s) 333, 495
StyD4I CCNGG 3 cut(s) 882, 904, 1215
TaaI ACNGT 6 cut(s) 9, 205, 554, 692, 945, 1192
TaiI ACGT 1 cut(s) 249
TaqI TCGA 1 cut(s) 657
TasI AATT 6 cut(s) 424, 455, 468, 632, 1220, 1236
TfiI GAWTC 2 cut(s) 518, 571
Tru1I TTAA 8 cut(s) 62, 140, 159, 243, 291, 380, 732, 879
Tru9I TTAA 8 cut(s) 62, 140, 159, 243, 291, 380, 732, 879
TscAI CASTG 2 cut(s) 12, 697
TseFI GTSAC 2 cut(s) 3, 418
TseI GCWGC 1 cut(s) 1091
Tsp45I GTSAC 2 cut(s) 3, 418
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 2 cut(s) 12, 697
Van91I CCANNNNNTGG 2 cut(s) 1157, 1222
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 1 cut(s) 632
XspI CTAG 2 cut(s) 333, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.