AT5G18670

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013209
Physical Location & Seq
Forward (+)
6225956 .. 6228195
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18670.1

Sequence Viewer

Length: 1611 bp
ATGGAAGTTTCAGTGATTGGAAATCCTCAAGCGAGGATCTGCAGAGCAGAATTAGCTTACAGAGAGCTTGGATTTAGATTTGGCTCTGATGTAATCTCCGGTGAATCGAGAAATAGGGTTAGTTTCTGCAACCAAAGCTCTAAATGGAAAGAGATCGCGATACGTTGCTCTTCGAGATCTGTCAAATGTGAAGCCATCGTCTCCGATGACGCTTCTCCGTTTCTCAAATCCACTCCAAAATCTAAATCGCTCGAGAGTGTAAAATTATTTGTTGGGCTTCCGTTAGACACAGTTTCAGACTGCAACAATGTGAATCACTTGAAAGCTATTACAGCTGGGCTTAAAGCTTTGAAGCTACTTGGTGTAGAAGGTATTGAGTTACCTATCTTTTGGGGAGTTGTTGAGAAAGAAGCTGCTGGGAAATATGAATGGTCTGGGTACTTGGCAGTAGCTGAGATTGTTAAGAAAGTGGGACTTAAGCTTCATGCTTCACTTTCTTTCCATGGATCGAAACAAACAGAGATAGGTCTTCCTGATTGGGTGGCAAAGATTGGTGATGCTGAACCAGGGATCTATTTTACAGATAGATATGGACAACAGTACAAAGATTGTTTGTCGTTTGCTGTTGATGATGTTCCTGTTCTTGATGGGAAGACTCCTATGGAGGTTTACAGAGGTTTCTGTGAGAGCTTCAAGTCTGCTTTCGCAGATTACATGGGCAACACAATCACGGGAATCACATTAGGTTTGGGACCAGACGGTGAGCTGAAATATCCTTCTCATCAACATAATGCCAAGCTCTCTGGCGCGGGAGAGTTCCAGTGTTACGACAAACACATGCTTTCTGCTCTTAAAGGCTACGCTGAATCCACTGGAAACCCTCTTTGGGGTCTCGGTGGTCCTCACGATGCTCCTGCTTACGATCAACAGCCTAATTCCTCTTCATTCTTCTCAGACGGCGGGTCATGGGAATCTCAGTACGGCGATTTCTTCTTGTCTTGGTATTCGTCTCTTCTCACCTCCCACGCAGACCGAGTCCTCTCCGTTGCTTCATCTGCATTTAGCGGGATTGGAGTGCCTCTATGTGGGAAGCTACCTCTCTTACACCAATGGCACAAGCTAAGATCTCATCCTTCTGAGTTAACAGCTGGATTCTACAGCTCTAATGGTCAGGACAGGTACGAGGCTATCGCAGAGATCTTTGCAAAGAACTCTTGTAGAATGATAATACCAGGAATGGACCTATCCGACGAGCACCAATCACCTGAATCTCTCTCGAGCCCCGAGTCATTACTTGGCCACATCAAAACTTCCTGCAAGAAACAAGGAGTCGTTGTCTCAGGGCAAAACTCATCCACCCCGGTTCCTGGTGGGTTTGAGAGGATCGTTGAGAATCTGAAGGATGAGAATGTAGGAATTGATCTGTTCACTTACCAGAGAATGGGAGCACTTTTCTTCTCTCCAGAGCATTTCCATGCTTTCACAGTCTTTGTCCGGAACCTGAGCCAATTCGAGTTGTCCTCAGACGATCAAGCCTCAGAGGCTGAGGTTGAGGCCGAGACAGCTAGCATAGGTTCAGGCACTGGTGCACCTAGTTTGCAAACCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

536

Amino Acids

58.28

Weight (kDa)

5.51

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 90 - 486 9.5e-91 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1441
AccII CGCG 2 cut(s) 158, 809
AccIII TCCGGA 1 cut(s) 1494
AciI CCGC 4 cut(s) 809, 960, 1065, 1605
AclWI GGATC 4 cut(s) 44, 514, 578, 1391
AcoI YGGCCR 1 cut(s) 1297
AcuI CTGAAG 1 cut(s) 1418
AfaI GTAC 4 cut(s) 440, 602, 980, 1181
AfiI CCNNNNNNNGG 5 cut(s) 886, 887, 1085, 1367, 1441
AflII CTTAAG 1 cut(s) 476
AgsI TTSAA 3 cut(s) 322, 352, 694
AhdI GACNNNNNGTC 1 cut(s) 961
AjnI CCWGG 3 cut(s) 565, 1231, 1366
AjuI GAANNNNNNNTTGG 2 cut(s) 868, 900
Alw21I GWGCWC 3 cut(s) 1257, 1450, 1591
Alw26I GTCTC 5 cut(s) 205, 896, 1014, 1342, 1553
Alw44I GTGCAC 1 cut(s) 1587
AlwI GGATC 4 cut(s) 44, 514, 578, 1391
AlwNI CAGNNNCTG 3 cut(s) 452, 1544, 1583
Ama87I CYCGRG 3 cut(s) 251, 1276, 1283
Aor13HI TCCGGA 1 cut(s) 1494
AoxI GGCC 2 cut(s) 1297, 1554
ApaLI GTGCAC 1 cut(s) 1587
ApeKI GCWGC 1 cut(s) 413
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 3 cut(s) 752, 899, 1240
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 5 cut(s) 113, 566, 773, 1009, 1254
AsuNHI GCTAGC 1 cut(s) 1565
AvaI CYCGRG 3 cut(s) 251, 1276, 1283
AvaII GGWCC 3 cut(s) 752, 899, 1240
BaeGI GKGCMC 1 cut(s) 1591
BalI TGGCCA 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1283
BbsI GAAGAC 2 cut(s) 521, 659
Bbv12I GWGCWC 3 cut(s) 1257, 1450, 1591
BbvCI CCTCAGC 1 cut(s) 1545
BbvI GCAGC 1 cut(s) 400
BccI CCATC 2 cut(s) 203, 641
BceAI ACGGC 2 cut(s) 973, 997
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 3 cut(s) 567, 1233, 1368
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 5 cut(s) 205, 896, 1014, 1342, 1553
BfaI CTAG 2 cut(s) 1566, 1593
BfmI CTRYAG 2 cut(s) 40, 1156
BfrI CTTAAG 1 cut(s) 476
BglI GCCNNNNNGGC 1 cut(s) 1541
BglII AGATCT 3 cut(s) 176, 1124, 1197
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
Bme1390I CCNGG 4 cut(s) 567, 1233, 1361, 1368
Bme18I GGWCC 3 cut(s) 752, 899, 1240
BmeRI GACNNNNNGTC 1 cut(s) 961
BmeT110I CYCGRG 3 cut(s) 251, 1276, 1283
BmgT120I GGNCC 3 cut(s) 752, 899, 1240
BmiI GGNNCC 3 cut(s) 753, 1365, 1499
BmrFI CCNGG 4 cut(s) 567, 1233, 1361, 1368
BmsI GCATC 2 cut(s) 547, 898
BmtI GCTAGC 1 cut(s) 1569
BpiI GAAGAC 2 cut(s) 521, 659
BplI GAGNNNNNCTC 2 cut(s) 1505, 1537
BpmI CTGGAG 1 cut(s) 1446
Bpu10I CCTNAGC 2 cut(s) 1502, 1545
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 1361
BsaI GGTCTC 1 cut(s) 896
BsaJI CCNNGG 3 cut(s) 502, 566, 1359
BsaWI WCCGGW 2 cut(s) 98, 1494
BsaXI ACNNNNNCTCC 2 cut(s) 1320, 1350
Bsc4I CCNNNNNNNGG 5 cut(s) 886, 887, 1085, 1367, 1441
Bse1I ACTGG 3 cut(s) 820, 877, 1588
BseAI TCCGGA 1 cut(s) 1494
BseBI CCWGG 3 cut(s) 567, 1233, 1368
BseDI CCNNGG 3 cut(s) 502, 566, 1359
BseGI GGATG 3 cut(s) 1129, 1352, 1408
BseLI CCNNNNNNNGG 5 cut(s) 886, 887, 1085, 1367, 1441
BseMII CTCAG 9 cut(s) 444, 966, 989, 1128, 1353, 1493, 1536, 1536, 1551
BseNI ACTGG 3 cut(s) 820, 877, 1588
BseSI GKGCMC 1 cut(s) 1591
BseXI GCAGC 1 cut(s) 400
BseYI CCCAGC 2 cut(s) 335, 416
Bsh1236I CGCG 2 cut(s) 158, 809
BshFI GGCC 2 cut(s) 1299, 1556
BsiHKAI GWGCWC 3 cut(s) 1257, 1450, 1591
BsiHKCI CYCGRG 3 cut(s) 251, 1276, 1283
BsiSI CCGG 3 cut(s) 99, 1361, 1495
BslFI GGGAC 2 cut(s) 486, 765
BslI CCNNNNNNNGG 5 cut(s) 886, 887, 1085, 1367, 1441
BsmAI GTCTC 5 cut(s) 205, 896, 1014, 1342, 1553
BsmBI CGTCTC 2 cut(s) 205, 1014
BsmFI GGGAC 2 cut(s) 486, 765
BsnI GGCC 2 cut(s) 1299, 1556
Bso31I GGTCTC 1 cut(s) 896
BsoBI CYCGRG 3 cut(s) 251, 1276, 1283
Bsp1286I GDGCHC 4 cut(s) 1257, 1283, 1450, 1591
Bsp13I TCCGGA 1 cut(s) 1494
Bsp19I CCATGG 1 cut(s) 502
Bsp68I TCGCGA 1 cut(s) 158
BspACI CCGC 4 cut(s) 809, 960, 1065, 1605
BspANI GGCC 2 cut(s) 1299, 1556
BspCNI CTCAG 9 cut(s) 445, 965, 988, 1129, 1352, 1494, 1535, 1537, 1550
BspEI TCCGGA 1 cut(s) 1494
BspFNI CGCG 2 cut(s) 158, 809
BspLI GGNNCC 3 cut(s) 753, 1365, 1499
BspMAI CTGCAG 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 1569
BspPI GGATC 4 cut(s) 44, 514, 578, 1391
BspQI GCTCTTC 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 896
BsrI ACTGG 3 cut(s) 820, 877, 1588
BssECI CCNNGG 3 cut(s) 502, 566, 1359
BssT1I CCWWGG 1 cut(s) 502
Bst2UI CCWGG 3 cut(s) 567, 1233, 1368
Bst4CI ACNGT 4 cut(s) 292, 600, 761, 1486
Bst6I CTCTTC 3 cut(s) 175, 946, 1017
BstAFI CTTAAG 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 1567
BstDSI CCRYGG 1 cut(s) 502
BstF5I GGATG 3 cut(s) 1129, 1352, 1408
BstFNI CGCG 2 cut(s) 158, 809
BstHHI GCGC 1 cut(s) 809
BstMAI GTCTC 5 cut(s) 205, 896, 1014, 1342, 1553
BstMWI GCNNNNNNNGC 6 cut(s) 53, 135, 332, 1055, 1541, 1562
BstNI CCWGG 3 cut(s) 567, 1233, 1368
BstNSI RCATGY 1 cut(s) 841
BstSCI CCNGG 4 cut(s) 565, 1231, 1359, 1366
BstSFI CTRYAG 2 cut(s) 40, 1156
BstSLI GKGCMC 1 cut(s) 1591
BstUI CGCG 2 cut(s) 158, 809
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 2 cut(s) 521, 659
BstX2I RGATCY 5 cut(s) 36, 176, 570, 1124, 1197
BstYI RGATCY 5 cut(s) 36, 176, 570, 1124, 1197
BsuRI GGCC 2 cut(s) 1299, 1556
BtgI CCRYGG 1 cut(s) 502
BtsCI GGATG 3 cut(s) 1129, 1352, 1408
BtsIMutI CAGTG 4 cut(s) 18, 827, 870, 1581
BtuMI TCGCGA 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 1567
CaiI CAGNNNCTG 3 cut(s) 452, 1544, 1583
CfoI GCGC 1 cut(s) 809
Cfr13I GGNCC 3 cut(s) 752, 899, 1240
CseI GACGC 1 cut(s) 218
Csp6I GTAC 4 cut(s) 439, 601, 979, 1180
CviAII CATG 6 cut(s) 485, 503, 715, 838, 966, 1475
CviQI GTAC 4 cut(s) 439, 601, 979, 1180
DriI GACNNNNNGTC 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 1297
Eam1104I CTCTTC 3 cut(s) 175, 946, 1017
Eam1105I GACNNNNNGTC 1 cut(s) 961
EarI CTCTTC 3 cut(s) 175, 946, 1017
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 1283
Eco31I GGTCTC 1 cut(s) 896
Eco47I GGWCC 3 cut(s) 752, 899, 1240
Eco57I CTGAAG 1 cut(s) 1418
Eco88I CYCGRG 3 cut(s) 251, 1276, 1283
EcoRII CCWGG 3 cut(s) 565, 1231, 1366
EcoT14I CCWWGG 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 1283
ErhI CCWWGG 1 cut(s) 502
Esp3I CGTCTC 2 cut(s) 205, 1014
FaeI CATG 6 cut(s) 488, 506, 718, 841, 969, 1478
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 2 cut(s) 486, 765
FatI CATG 6 cut(s) 484, 502, 714, 837, 965, 1474
FauI CCCGC 3 cut(s) 802, 953, 1058
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 3 cut(s) 1116, 1339, 1415
FriOI GRGCYC 1 cut(s) 1283
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 2 cut(s) 1566, 1593
GlaI GCGC 1 cut(s) 808
GluI GCNGC 1 cut(s) 414
GsaI CCCAGC 2 cut(s) 339, 420
GsuI CTGGAG 1 cut(s) 1446
HaeIII GGCC 2 cut(s) 1299, 1556
HapII CCGG 3 cut(s) 99, 1361, 1495
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 809
Hin1II CATG 6 cut(s) 488, 506, 718, 841, 969, 1478
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 1 cut(s) 1143
HindII GTYRAC 1 cut(s) 1143
HindIII AAGCTT 2 cut(s) 345, 479
HpaI GTTAAC 1 cut(s) 1143
HpaII CCGG 3 cut(s) 99, 1361, 1495
HphI GGTGA 5 cut(s) 113, 566, 773, 1009, 1254
Hpy166II GTNNAC 4 cut(s) 670, 1143, 1428, 1589
Hpy188I TCNGA 9 cut(s) 88, 205, 298, 955, 1138, 1249, 1398, 1525, 1540
Hpy8I GTNNAC 4 cut(s) 670, 1143, 1428, 1589
Hpy99I CGWCG 1 cut(s) 1253
HpyAV CCTTC 4 cut(s) 362, 786, 1143, 1393
HpyCH4III ACNGT 4 cut(s) 292, 600, 761, 1486
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 8 cut(s) 42, 129, 303, 1058, 1205, 1317, 1589, 1600
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 135, 332, 1055, 1541, 1562
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 6 cut(s) 488, 506, 718, 841, 969, 1478
HspAI GCGC 1 cut(s) 807
Kpn2I TCCGGA 1 cut(s) 1494
KspAI GTTAAC 1 cut(s) 1143
LguI GCTCTTC 1 cut(s) 175
LmnI GCTCC 2 cut(s) 916, 1445
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 2 cut(s) 547, 898
MaeI CTAG 2 cut(s) 1566, 1593
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 2 cut(s) 378, 824
MboII GAAGA 8 cut(s) 162, 521, 664, 933, 940, 982, 1004, 1447
MflI RGATCY 5 cut(s) 36, 176, 570, 1124, 1197
MhlI GDGCHC 4 cut(s) 1257, 1283, 1450, 1591
MlsI TGGCCA 1 cut(s) 1299
MluCI AATT 5 cut(s) 50, 263, 934, 1416, 1508
MluNI TGGCCA 1 cut(s) 1299
MlyI GAGTC 4 cut(s) 649, 1044, 1295, 1338
MmeI TCCRAC 1 cut(s) 1272
Mox20I TGGCCA 1 cut(s) 1299
MroI TCCGGA 1 cut(s) 1494
MscI TGGCCA 1 cut(s) 1299
MseI TTAA 6 cut(s) 342, 462, 477, 852, 1142, 1609
MslI CAYNNNNRTG 1 cut(s) 1473
Msp20I TGGCCA 1 cut(s) 1299
MspA1I CMGCKG 2 cut(s) 335, 1148
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 3 cut(s) 99, 1361, 1495
MspR9I CCNGG 4 cut(s) 567, 1233, 1361, 1368
MvaI CCWGG 3 cut(s) 567, 1233, 1368
MvnI CGCG 2 cut(s) 158, 809
MwoI GCNNNNNNNGC 6 cut(s) 53, 135, 332, 1055, 1541, 1562
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 502
NheI GCTAGC 1 cut(s) 1565
NlaIII CATG 6 cut(s) 488, 506, 718, 841, 969, 1478
NlaIV GGNNCC 3 cut(s) 753, 1365, 1499
NmeAIII GCCGAG 1 cut(s) 1582
NruI TCGCGA 1 cut(s) 158
NspI RCATGY 1 cut(s) 841
PaeR7I CTCGAG 2 cut(s) 251, 1276
PciSI GCTCTTC 1 cut(s) 175
PfeI GAWTC 8 cut(s) 104, 313, 735, 866, 971, 1152, 1268, 1393
PflFI GACNNNGTC 1 cut(s) 1034
PflMI CCANNNNNTGG 1 cut(s) 1441
PkrI GCNGC 1 cut(s) 415
PleI GAGTC 4 cut(s) 649, 1043, 1294, 1337
PpsI GAGTC 4 cut(s) 649, 1043, 1294, 1337
Psp6I CCWGG 3 cut(s) 565, 1231, 1366
PspFI CCCAGC 2 cut(s) 335, 416
PspGI CCWGG 3 cut(s) 565, 1231, 1366
PspN4I GGNNCC 3 cut(s) 753, 1365, 1499
PspPI GGNCC 3 cut(s) 752, 899, 1240
PstI CTGCAG 1 cut(s) 44
PstNI CAGNNNCTG 3 cut(s) 452, 1544, 1583
PsuI RGATCY 5 cut(s) 36, 176, 570, 1124, 1197
PsyI GACNNNGTC 1 cut(s) 1034
PvuII CAGCTG 2 cut(s) 335, 1148
RruI TCGCGA 1 cut(s) 158
RsaI GTAC 4 cut(s) 440, 602, 980, 1181
RsaNI GTAC 4 cut(s) 439, 601, 979, 1180
RseI CAYNNNNRTG 1 cut(s) 1473
SapI GCTCTTC 1 cut(s) 175
SaqAI TTAA 6 cut(s) 342, 462, 477, 852, 1142, 1609
SatI GCNGC 1 cut(s) 414
Sau96I GGNCC 3 cut(s) 752, 899, 1240
SchI GAGTC 4 cut(s) 649, 1044, 1295, 1338
ScrFI CCNGG 4 cut(s) 567, 1233, 1361, 1368
SduI GDGCHC 4 cut(s) 1257, 1283, 1450, 1591
SfaNI GCATC 2 cut(s) 547, 898
SfcI CTRYAG 2 cut(s) 40, 1156
Sfr274I CTCGAG 2 cut(s) 251, 1276
SinI GGWCC 3 cut(s) 752, 899, 1240
SlaI CTCGAG 2 cut(s) 251, 1276
SmiMI CAYNNNNRTG 1 cut(s) 1473
SmlI CTYRAG 4 cut(s) 27, 251, 476, 1276
SmoI CTYRAG 4 cut(s) 27, 251, 476, 1276
Sse9I AATT 5 cut(s) 50, 263, 934, 1416, 1508
SsiI CCGC 4 cut(s) 809, 960, 1065, 1605
SspMI CTAG 2 cut(s) 1566, 1593
StyD4I CCNGG 4 cut(s) 565, 1231, 1359, 1366
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 4 cut(s) 292, 600, 761, 1486
TaiI ACGT 1 cut(s) 166
TaqI TCGA 6 cut(s) 107, 173, 252, 509, 1277, 1512
TaqII GACCGA 1 cut(s) 1047
TasI AATT 5 cut(s) 50, 263, 934, 1416, 1508
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 8 cut(s) 104, 313, 735, 866, 971, 1152, 1268, 1393
Tru1I TTAA 6 cut(s) 342, 462, 477, 852, 1142, 1609
Tru9I TTAA 6 cut(s) 342, 462, 477, 852, 1142, 1609
TscAI CASTG 4 cut(s) 18, 827, 877, 1588
TseI GCWGC 1 cut(s) 413
TspDTI ATGAA 4 cut(s) 441, 473, 933, 1041
TspGWI ACGGA 3 cut(s) 207, 270, 1033
TspRI CASTG 4 cut(s) 18, 827, 877, 1588
Tth111I GACNNNGTC 1 cut(s) 1034
Van91I CCANNNNNTGG 1 cut(s) 1441
Vha464I CTTAAG 1 cut(s) 476
VneI GTGCAC 1 cut(s) 1587
VpaK11BI GGWCC 3 cut(s) 752, 899, 1240
XceI RCATGY 1 cut(s) 841
XhoI CTCGAG 2 cut(s) 251, 1276
XspI CTAG 2 cut(s) 1566, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.