Rh4DG227200

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013209
Physical Location & Seq
Reverse (-)
43162967 .. 43165815
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG227200.1

Sequence Viewer

Length: 1275 bp
ATGGCTCTGAAGCTATTGGGAGTGACAGGCGTGGAGCTTCCTGTCTGGTGGGGAATTGTTGAGAAAGAAGCCATGGGGAAGTATGAGTGGTCTGCCTACCATTCTTTAGCAGAGATGGTTCAGAAAGCCGGTCTTGAGCTTCATGTGTCACTCTGCTTCCACGCTTCTAAACAACCGAAGATCTCACTCCCCGATTGGGTGTCTTGCTTAGGGGAGTCCCAACCTGGTATGTTTTTCAAAGACAGGTCAGGGCAGCAGTTCAAGGAGTGTTTATCACTGGCTGTTGATGAGCTTCCTGTTCTTAATGGAAAGACTCCGATTCAAGTTTACCATGATTTCTGTGAAAGCTTTAAAGCTTCGTTCTCGCCTTTCCTTGGTTCCACAATCACGGGCGTCTCAGTGAGCCTAGGACCAGATGGTGAGCTTCGGTATCCTTCTCATCACCAGTCAGTCAAACGTAGCAAAATTCCCGGGGTTGGCGAATTCCAATGTTTTGATGAAAACATGCTCAGTGCTCTTAAACAACATGCTGAGGCAACCGGAAATCCTTTATGGGGTCTTGGCGGCCCCCATGATGCTCCGAGCTATGACCAGTCACCGGACTCAAACACTTTCTTCAAGGACCATGGGGGATCATGGGAGTCACCATATGGTGATTTCTTCCTGTCCTGGTACTCAAACCAGCTAATCTCTCATGGAGATCGGATCCTTTCCCTTGCTTCTTCAACTTTTGGTGACACAGAAGTCACAGTATATGGCAAAGTCCCTCTAATGCACTCTTGGTACAAAACAAGGTCTCACCCATCCGAGTTGACGTCTGGGTTTTATAATACATCTTCTCGAGATGGTTATGAAGCAGTTGCAGATATGTTTGCAAGAAACTCCTGCAAAATGATATTGCCGGGACTGGACTTATCAGACGTACATCAACCACACGAATCCCGTTCTAGTCCCGAGTCATTACTATCACAAATTAGAACAGCGTGCAAAAAGCATGGAATTGAAATTTCTGGTCAAAACTCATCAATTTCAGGAGCTCCAGGAGGCTTTCAACAGATAAAGAAGAACTTGTTAGGAGAGAATAAAATAGACTTGTTCACTTACCAGAGAATGGGTGCTTATTTCTTCTCACCTGAGCATTTTCCTTCATTTTCTGGATTTGTCCGAAGCCTGAATCAAGTGGAATTGCAGTCAGATGATCTAGCTTCTGACGAAGAAGCTACTGAATCTATTCATGTCAACTCAGAACCAGGAATCCACATGCAAGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

424

Amino Acids

46.84

Weight (kDa)

5.54

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 376 3.2e-92 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AasI GACNNNNNNGTC 1 cut(s) 743
AatII GACGTC 1 cut(s) 818
AccB7I CCANNNNNTGG 1 cut(s) 1111
AciI CCGC 2 cut(s) 564, 1269
AclWI GGATC 3 cut(s) 640, 700, 713
AcsI RAATTY 3 cut(s) 465, 482, 1005
AcuI CTGAAG 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 393, 815
AfaI GTAC 3 cut(s) 674, 785, 924
AfiI CCNNNNNNNGG 6 cut(s) 196, 374, 476, 554, 598, 1111
AgsI TTSAA 7 cut(s) 238, 262, 323, 619, 726, 1004, 1052
AjnI CCWGG 4 cut(s) 223, 668, 1039, 1249
AloI GAACNNNNNNTCC 2 cut(s) 1239, 1271
Alw21I GWGCWC 2 cut(s) 517, 1039
Alw26I GTCTC 2 cut(s) 400, 801
AlwI GGATC 3 cut(s) 640, 700, 713
Ama87I CYCGRG 3 cut(s) 470, 840, 953
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 3 cut(s) 465, 482, 1005
Asp700I GAANNNNTTC 1 cut(s) 1230
AspA2I CCTAGG 1 cut(s) 406
AspS9I GGNCC 3 cut(s) 410, 566, 622
AsuC2I CCSGG 3 cut(s) 471, 472, 903
AsuHPI GGTGA 8 cut(s) 431, 434, 588, 636, 665, 746, 791, 1122
AvaI CYCGRG 3 cut(s) 470, 840, 953
AvaII GGWCC 2 cut(s) 410, 622
AvrII CCTAGG 1 cut(s) 406
BamHI GGATCC 1 cut(s) 705
BanII GRGCYC 1 cut(s) 1039
Bbv12I GWGCWC 2 cut(s) 517, 1039
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 265
BccI CCATC 4 cut(s) 109, 410, 811, 839
BciT130I CCWGG 4 cut(s) 225, 670, 1041, 1251
BciVI GTATCC 1 cut(s) 441
BcnI CCSGG 3 cut(s) 471, 472, 903
BcoDI GTCTC 2 cut(s) 400, 801
BfaI CTAG 3 cut(s) 407, 948, 1202
BfuI GTATCC 1 cut(s) 441
BglII AGATCT 1 cut(s) 180
BisI GCNGC 3 cut(s) 254, 565, 1269
BlnI CCTAGG 1 cut(s) 406
BlsI GCNGC 3 cut(s) 255, 566, 1270
Bme1390I CCNGG 7 cut(s) 225, 471, 472, 670, 903, 1041, 1251
Bme18I GGWCC 2 cut(s) 410, 622
BmeT110I CYCGRG 3 cut(s) 470, 840, 953
BmgT120I GGNCC 3 cut(s) 410, 566, 622
BmiI GGNNCC 3 cut(s) 379, 568, 707
BmrFI CCNGG 7 cut(s) 225, 471, 472, 670, 903, 1041, 1251
BmsI GCATC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 1023
Bpu10I CCTNAGC 3 cut(s) 208, 531, 1134
BpuEI CTTGAG 1 cut(s) 155
BpuMI CCSGG 3 cut(s) 471, 472, 903
BsaHI GRCGYC 2 cut(s) 393, 815
BsaI GGTCTC 1 cut(s) 801
BsaJI CCNNGG 6 cut(s) 72, 373, 406, 470, 471, 625
BsaWI WCCGGW 2 cut(s) 539, 598
Bsc4I CCNNNNNNNGG 6 cut(s) 196, 374, 476, 554, 598, 1111
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 4 cut(s) 282, 445, 592, 912
BseBI CCWGG 4 cut(s) 225, 670, 1041, 1251
BseDI CCNNGG 6 cut(s) 72, 373, 406, 470, 471, 625
BseGI GGATG 1 cut(s) 803
BseLI CCNNNNNNNGG 6 cut(s) 196, 374, 476, 554, 598, 1111
BseMII CTCAG 5 cut(s) 411, 522, 523, 1125, 1257
BseNI ACTGG 4 cut(s) 282, 445, 592, 912
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 517, 1039
BsiHKCI CYCGRG 3 cut(s) 470, 840, 953
BsiSI CCGG 5 cut(s) 129, 471, 540, 599, 902
BslFI GGGAC 4 cut(s) 202, 749, 918, 936
BslI CCNNNNNNNGG 6 cut(s) 196, 374, 476, 554, 598, 1111
BsmAI GTCTC 2 cut(s) 400, 801
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 4 cut(s) 202, 749, 918, 936
BsnI GGCC 1 cut(s) 567
Bso31I GGTCTC 1 cut(s) 801
BsoBI CYCGRG 3 cut(s) 470, 840, 953
Bsp1286I GDGCHC 2 cut(s) 517, 1039
Bsp143I GATC 5 cut(s) 180, 632, 700, 705, 1198
Bsp19I CCATGG 2 cut(s) 72, 625
BspACI CCGC 2 cut(s) 564, 1269
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 5 cut(s) 410, 522, 523, 1126, 1256
BspLI GGNNCC 3 cut(s) 379, 568, 707
BspPI GGATC 3 cut(s) 640, 700, 713
BspTNI GGTCTC 1 cut(s) 801
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 4 cut(s) 282, 445, 592, 912
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 6 cut(s) 72, 373, 406, 470, 471, 625
BssMI GATC 5 cut(s) 180, 632, 700, 705, 1198
BssNI GRCGYC 2 cut(s) 393, 815
BssT1I CCWWGG 4 cut(s) 72, 373, 406, 625
Bst2UI CCWGG 4 cut(s) 225, 670, 1041, 1251
Bst4CI ACNGT 1 cut(s) 751
BstACI GRCGYC 2 cut(s) 393, 815
BstC8I GCNNGC 2 cut(s) 985, 1266
BstDEI CTNAG 7 cut(s) 208, 397, 509, 531, 1134, 1243, 1272
BstDSI CCRYGG 2 cut(s) 72, 625
BstF5I GGATG 1 cut(s) 803
BstKTI GATC 5 cut(s) 183, 635, 703, 708, 1201
BstMAI GTCTC 2 cut(s) 400, 801
BstMBI GATC 5 cut(s) 180, 632, 700, 705, 1198
BstNI CCWGG 4 cut(s) 225, 670, 1041, 1251
BstNSI RCATGY 3 cut(s) 508, 530, 1264
BstSCI CCNGG 7 cut(s) 223, 469, 470, 668, 901, 1039, 1249
BstV1I GCAGC 1 cut(s) 265
BstX2I RGATCY 2 cut(s) 180, 705
BstYI RGATCY 2 cut(s) 180, 705
BsuI GTATCC 1 cut(s) 441
BsuRI GGCC 1 cut(s) 567
BtgI CCRYGG 2 cut(s) 72, 625
BtsCI GGATG 1 cut(s) 803
BtsIMutI CAGTG 3 cut(s) 275, 405, 517
Cac8I GCNNGC 2 cut(s) 985, 1266
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 3 cut(s) 410, 566, 622
Cfr9I CCCGGG 1 cut(s) 470
CseI GACGC 1 cut(s) 382
CsiI ACCWGGT 1 cut(s) 223
Csp6I GTAC 3 cut(s) 673, 784, 923
CviQI GTAC 3 cut(s) 673, 784, 923
DdeI CTNAG 7 cut(s) 208, 397, 509, 531, 1134, 1243, 1272
DpnI GATC 5 cut(s) 182, 634, 702, 707, 1200
DpnII GATC 5 cut(s) 180, 632, 700, 705, 1198
DraI TTTAAA 1 cut(s) 352
DrdI GACNNNNNNGTC 1 cut(s) 743
DseDI GACNNNNNNGTC 1 cut(s) 743
Ecl136II GAGCTC 1 cut(s) 1037
Eco130I CCWWGG 4 cut(s) 72, 373, 406, 625
Eco24I GRGCYC 1 cut(s) 1039
Eco31I GGTCTC 1 cut(s) 801
Eco47I GGWCC 2 cut(s) 410, 622
Eco53kI GAGCTC 1 cut(s) 1037
Eco57I CTGAAG 1 cut(s) 29
Eco88I CYCGRG 3 cut(s) 470, 840, 953
EcoICRI GAGCTC 1 cut(s) 1037
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 4 cut(s) 223, 668, 1039, 1249
EcoT14I CCWWGG 4 cut(s) 72, 373, 406, 625
EcoT38I GRGCYC 1 cut(s) 1039
ErhI CCWWGG 4 cut(s) 72, 373, 406, 625
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 4 cut(s) 117, 149, 1052, 1084
FaqI GGGAC 4 cut(s) 202, 749, 918, 936
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 3 cut(s) 254, 565, 1269
FokI GGATG 1 cut(s) 790
FriOI GRGCYC 1 cut(s) 1039
Fsp4HI GCNGC 3 cut(s) 254, 565, 1269
FspBI CTAG 3 cut(s) 407, 948, 1202
GluI GCNGC 3 cut(s) 254, 565, 1269
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 1 cut(s) 567
HapII CCGG 5 cut(s) 129, 471, 540, 599, 902
HgaI GACGC 1 cut(s) 382
Hin1I GRCGYC 2 cut(s) 393, 815
HincII GTYRAC 2 cut(s) 813, 1240
HindII GTYRAC 2 cut(s) 813, 1240
HindIII AAGCTT 2 cut(s) 346, 354
HpaII CCGG 5 cut(s) 129, 471, 540, 599, 902
HphI GGTGA 8 cut(s) 431, 434, 588, 636, 665, 746, 791, 1122
Hpy166II GTNNAC 4 cut(s) 328, 813, 1098, 1240
Hpy188III TCNNGA 6 cut(s) 134, 840, 842, 953, 1032, 1155
Hpy8I GTNNAC 4 cut(s) 328, 813, 1098, 1240
HpyAV CCTTC 2 cut(s) 444, 1155
HpyCH4III ACNGT 1 cut(s) 751
HpyCH4IV ACGT 3 cut(s) 457, 815, 921
HpyCH4V TGCA 7 cut(s) 775, 863, 875, 888, 987, 1189, 1264
HpyF3I CTNAG 7 cut(s) 208, 397, 509, 531, 1134, 1243, 1272
HpySE526I ACGT 3 cut(s) 457, 815, 921
Hsp92I GRCGYC 2 cut(s) 393, 815
Kzo9I GATC 5 cut(s) 180, 632, 700, 705, 1198
LmnI GCTCC 4 cut(s) 34, 583, 1034, 1042
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 1 cut(s) 565
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 3 cut(s) 407, 948, 1202
MaeII ACGT 3 cut(s) 457, 815, 921
MaeIII GTNAC 6 cut(s) 22, 147, 594, 642, 734, 745
MalI GATC 5 cut(s) 182, 634, 702, 707, 1200
MboI GATC 5 cut(s) 180, 632, 700, 705, 1198
MboII GAAGA 8 cut(s) 190, 607, 652, 714, 828, 1075, 1117, 1226
MflI RGATCY 2 cut(s) 180, 705
MhlI GDGCHC 2 cut(s) 517, 1039
MluCI AATT 8 cut(s) 54, 465, 482, 972, 999, 1005, 1026, 1184
MlyI GAGTC 5 cut(s) 224, 307, 596, 650, 965
MnlI CCTC 3 cut(s) 526, 777, 1037
MroXI GAANNNNTTC 1 cut(s) 1230
MseI TTAA 3 cut(s) 303, 351, 519
MspI CCGG 5 cut(s) 129, 471, 540, 599, 902
MspR9I CCNGG 7 cut(s) 225, 471, 472, 670, 903, 1041, 1251
MvaI CCWGG 4 cut(s) 225, 670, 1041, 1251
NciI CCSGG 3 cut(s) 471, 472, 903
NcoI CCATGG 2 cut(s) 72, 625
NdeI CATATG 1 cut(s) 649
NdeII GATC 5 cut(s) 180, 632, 700, 705, 1198
NlaIV GGNNCC 3 cut(s) 379, 568, 707
NmuCI GTSAC 6 cut(s) 22, 147, 594, 642, 734, 745
NspI RCATGY 3 cut(s) 508, 530, 1264
PaeR7I CTCGAG 1 cut(s) 840
PdmI GAANNNNTTC 1 cut(s) 1230
PfeI GAWTC 5 cut(s) 319, 938, 1174, 1226, 1254
PflMI CCANNNNNTGG 1 cut(s) 1111
PfoI TCCNGGA 1 cut(s) 1039
PkrI GCNGC 3 cut(s) 255, 566, 1270
PleI GAGTC 5 cut(s) 223, 307, 596, 649, 964
PpsI GAGTC 5 cut(s) 223, 307, 596, 649, 964
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 1039
Psp6I CCWGG 4 cut(s) 223, 668, 1039, 1249
PspGI CCWGG 4 cut(s) 223, 668, 1039, 1249
PspN4I GGNNCC 3 cut(s) 379, 568, 707
PspPI GGNCC 3 cut(s) 410, 566, 622
PsuI RGATCY 2 cut(s) 180, 705
RsaI GTAC 3 cut(s) 674, 785, 924
RsaNI GTAC 3 cut(s) 673, 784, 923
SacI GAGCTC 1 cut(s) 1039
SaqAI TTAA 3 cut(s) 303, 351, 519
SatI GCNGC 3 cut(s) 254, 565, 1269
Sau3AI GATC 5 cut(s) 180, 632, 700, 705, 1198
Sau96I GGNCC 3 cut(s) 410, 566, 622
SchI GAGTC 5 cut(s) 224, 307, 596, 650, 965
ScrFI CCNGG 7 cut(s) 225, 471, 472, 670, 903, 1041, 1251
SduI GDGCHC 2 cut(s) 517, 1039
SexAI ACCWGGT 1 cut(s) 223
SfaNI GCATC 1 cut(s) 565
Sfr274I CTCGAG 1 cut(s) 840
SinI GGWCC 2 cut(s) 410, 622
SlaI CTCGAG 1 cut(s) 840
SmaI CCCGGG 1 cut(s) 472
SmlI CTYRAG 2 cut(s) 134, 840
SmoI CTYRAG 2 cut(s) 134, 840
Sse9I AATT 8 cut(s) 54, 465, 482, 972, 999, 1005, 1026, 1184
SsiI CCGC 2 cut(s) 564, 1269
SspMI CTAG 3 cut(s) 407, 948, 1202
SstI GAGCTC 1 cut(s) 1039
StyD4I CCNGG 7 cut(s) 223, 469, 470, 668, 901, 1039, 1249
StyI CCWWGG 4 cut(s) 72, 373, 406, 625
TaaI ACNGT 1 cut(s) 751
TaiI ACGT 3 cut(s) 460, 818, 924
TaqI TCGA 1 cut(s) 841
TasI AATT 8 cut(s) 54, 465, 482, 972, 999, 1005, 1026, 1184
TauI GCSGC 2 cut(s) 567, 1271
TfiI GAWTC 5 cut(s) 319, 938, 1174, 1226, 1254
Tru1I TTAA 3 cut(s) 303, 351, 519
Tru9I TTAA 3 cut(s) 303, 351, 519
TscAI CASTG 3 cut(s) 282, 405, 517
TseFI GTSAC 6 cut(s) 22, 147, 594, 642, 734, 745
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 6 cut(s) 22, 147, 594, 642, 734, 745
TspDTI ATGAA 5 cut(s) 131, 513, 867, 1137, 1223
TspMI CCCGGG 1 cut(s) 470
TspRI CASTG 3 cut(s) 282, 405, 517
Van91I CCANNNNNTGG 1 cut(s) 1111
VpaK11BI GGWCC 2 cut(s) 410, 622
XapI RAATTY 3 cut(s) 465, 482, 1005
XceI RCATGY 3 cut(s) 508, 530, 1264
XhoI CTCGAG 1 cut(s) 840
XmaI CCCGGG 1 cut(s) 470
XmaJI CCTAGG 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 1230
XspI CTAG 3 cut(s) 407, 948, 1202
ZraI GACGTC 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.