Rh4BG231800

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013209
Physical Location & Seq
Reverse (-)
39647982 .. 39650159
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG231800.1

Sequence Viewer

Length: 1593 bp
ATGGAGGTTTCTGTGGTTCGAAGTTCTCAGGTGAAGATCGGAAGGGCTGAATTGGGACACAGGGAGTTTGGGTTTTGTAAATTGAGTGGTAATTTGAGGACCCAGATAAGCTTTGGTCAAAAAAAGAGCTGGAAAAATGGCCGGCTTCAGTTTACTCTCAGGGCTGTTCAGTCTGAATCGGTTCGACCCGTTAAAGCTTCGGGTCCTTCCAAAAGACCCAAATCGAATGATGGGTTAAGACTATTCGTTGGGTTGCCCCTGGATGCAGTTTCAGACTGCAATTCGGTGAAGCATGCCCGAGCAATTGCGGTTGGACTAATGGCTCTGAAGCTATTGGGAGTGACAGGCGTGGAGCTTCCTGTCTGGTGGGGAATTGTTGAGAAAGAAGCCATGGGGAAGTATGAGTGGTCAGCCTACCATTCTTTAGCAGAGATGGTTCAGAAAGCCGGTCTTGAGCTTCATGTGTCACTCTGCTTCCATGCTTCTAAACAATCGAAGATCTCACTCCCCGATTGGGTGTCTCGCTTAGGGGAGTCCCAACCTGGTATGTTTTTCAAAGACAGGTCAGGGCAGCAATACAAGGAGTGTTTATCACTGGCTGTTGATGAGCTTCCTGTTCTTAATGGAAAGACTCCGATTCAAGTTTACCATGATTTCTGCGAAAGCTTTAAAGCTTCGTTCTCGCCTTTCCTTGGTTCCACAATCACGGGCGTCTCAGTGAGCCTAGGACCAGATGGTGAGCTTCGGTATCCTTCTCATCACCCGTCAGTCAAACGTAGCAAAATTCCCGGGGTTGGCGAATTCCAATGTTTTGATGAAAACATGCTCAATGTTCTTAAACAACATGCTGAGGCAACCGGAAATCCTTTATGGGGTCTTGGCGGTCCCCATGATGCTCCAAGCTATGACCAGTCACCGGACTCAAACACTTTCTTCAAGGACCATGGGGGATCATGGGAGTCACCATATGGTGATTTCTTCCTGTCCTGGTACTCAAACCAGCTAATCTCTCATGGAGATCGGATCCTTTCCCTTGCTTCTTCAACTTTTGGTGACACAGAAGTCACAGCATATGGCAAAGTCCCTCTAATTCACTCTTGGTACAAAACAAGGTCTCACCCATCCGAGTTGACATCTGGGTTTTATAATACATCCTCTCGAGATGGTTATGAAGCAGTTGCAGATATGTTTGCAAGAAACTCCTGCAAAATGATATTGCCAGGACTGGACTTATCGGACGTACATCAACCACACGAATCCCGTTCTAGTCCCGAGTCATTGCTATCACAAATTAGAACAGCGTGCAAAAAGCACGGAGTTGAAATTTCTGGTCAAAACTCATCAATTTCAGGAGCTCTAGGAGGCTTTCAACAGATAAAGAAGAACTTGTTAGGAGAGAATAAAATAGACTTGTTCACTTATCAGAGAATGGGAGCTTATTTCTTCTCACCCGAGCATTTTCCTTCATTTTCTGGATTCGTCCGAAGCCTTAATCAAGTGGAATTGCAGTCAGATGATCTAGCTTCTGACGAAGAAGCTACTGAATCTATTCATGTCAACTCAGAACCAGGAATCCACATGCAAGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

530

Amino Acids

58.44

Weight (kDa)

6.71

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 82 - 482 2e-97 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1146
AasI GACNNNNNNGTC 1 cut(s) 1061
AciI CCGC 3 cut(s) 308, 882, 1587
AclWI GGATC 3 cut(s) 958, 1018, 1031
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 3 cut(s) 783, 800, 1323
AcuI CTGAAG 2 cut(s) 131, 347
AcyI GRCGYC 1 cut(s) 711
AfaI GTAC 3 cut(s) 992, 1103, 1242
AfiI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 916
AgsI TTSAA 6 cut(s) 556, 641, 937, 1044, 1322, 1370
AjnI CCWGG 5 cut(s) 258, 541, 986, 1219, 1567
AloI GAACNNNNNNTCC 2 cut(s) 1557, 1589
Alw21I GWGCWC 1 cut(s) 1357
Alw26I GTCTC 3 cut(s) 525, 718, 1119
AlwI GGATC 3 cut(s) 958, 1018, 1031
Ama87I CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 571
ApoI RAATTY 3 cut(s) 783, 800, 1323
Asp700I GAANNNNTTC 2 cut(s) 180, 1548
AspA2I CCTAGG 1 cut(s) 724
AspS9I GGNCC 5 cut(s) 99, 203, 728, 884, 940
AsuC2I CCSGG 2 cut(s) 789, 790
AsuII TTCGAA 1 cut(s) 19
AvaI CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
AvaII GGWCC 5 cut(s) 99, 203, 728, 884, 940
AvrII CCTAGG 1 cut(s) 724
BamHI GGATCC 1 cut(s) 1023
BanII GRGCYC 1 cut(s) 1357
Bbv12I GWGCWC 1 cut(s) 1357
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 1 cut(s) 583
BccI CCATC 5 cut(s) 224, 427, 728, 1129, 1157
BciT130I CCWGG 5 cut(s) 260, 543, 988, 1221, 1569
BciVI GTATCC 1 cut(s) 759
BcnI CCSGG 2 cut(s) 789, 790
BcoDI GTCTC 3 cut(s) 525, 718, 1119
BfaI CTAG 4 cut(s) 725, 1266, 1358, 1520
BfuI GTATCC 1 cut(s) 759
BglII AGATCT 1 cut(s) 498
BisI GCNGC 2 cut(s) 572, 1587
BlnI CCTAGG 1 cut(s) 724
BlsI GCNGC 2 cut(s) 573, 1588
Bme1390I CCNGG 7 cut(s) 260, 543, 789, 790, 988, 1221, 1569
Bme18I GGWCC 5 cut(s) 99, 203, 728, 884, 940
BmeT110I CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
BmgT120I GGNCC 5 cut(s) 99, 203, 728, 884, 940
BmiI GGNNCC 5 cut(s) 101, 204, 697, 886, 1025
BmrFI CCNGG 7 cut(s) 260, 543, 789, 790, 988, 1221, 1569
BmsI GCATC 2 cut(s) 253, 883
Bpu10I CCTNAGC 2 cut(s) 526, 849
Bpu14I TTCGAA 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 789, 790
BsaHI GRCGYC 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 7 cut(s) 258, 390, 691, 724, 788, 789, 943
BsaWI WCCGGW 2 cut(s) 857, 916
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 916
Bse118I RCCGGY 2 cut(s) 141, 446
Bse1I ACTGG 3 cut(s) 600, 910, 1230
Bse3DI GCAATG 1 cut(s) 1277
BseBI CCWGG 5 cut(s) 260, 543, 988, 1221, 1569
BseDI CCNNGG 7 cut(s) 258, 390, 691, 724, 788, 789, 943
BseGI GGATG 3 cut(s) 268, 1121, 1151
BseLI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 916
BseMI GCAATG 1 cut(s) 1277
BseMII CTCAG 5 cut(s) 41, 172, 729, 840, 1575
BseNI ACTGG 3 cut(s) 600, 910, 1230
BseXI GCAGC 1 cut(s) 583
BshFI GGCC 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 1357
BsiHKCI CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
BsiSI CCGG 5 cut(s) 142, 447, 789, 858, 917
BslFI GGGAC 5 cut(s) 69, 520, 870, 1067, 1254
BslI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 916
BsmAI GTCTC 3 cut(s) 525, 718, 1119
BsmBI CGTCTC 1 cut(s) 718
BsmFI GGGAC 5 cut(s) 69, 520, 870, 1067, 1254
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 1119
BsoBI CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
Bsp119I TTCGAA 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 1357
Bsp143I GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
Bsp19I CCATGG 2 cut(s) 390, 943
BspACI CCGC 3 cut(s) 308, 882, 1587
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 5 cut(s) 40, 171, 728, 841, 1574
BspLI GGNNCC 5 cut(s) 101, 204, 697, 886, 1025
BspPI GGATC 3 cut(s) 958, 1018, 1031
BspT104I TTCGAA 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 1277
BsrFI RCCGGY 2 cut(s) 141, 446
BsrI ACTGG 3 cut(s) 600, 910, 1230
BssAI RCCGGY 2 cut(s) 141, 446
BssECI CCNNGG 7 cut(s) 258, 390, 691, 724, 788, 789, 943
BssMI GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
BssNI GRCGYC 1 cut(s) 711
BssT1I CCWWGG 4 cut(s) 390, 691, 724, 943
Bst2UI CCWGG 5 cut(s) 260, 543, 988, 1221, 1569
BstACI GRCGYC 1 cut(s) 711
BstBI TTCGAA 1 cut(s) 19
BstC8I GCNNGC 4 cut(s) 143, 294, 1303, 1584
BstDEI CTNAG 7 cut(s) 27, 158, 526, 715, 849, 1561, 1590
BstDSI CCRYGG 2 cut(s) 390, 943
BstF5I GGATG 3 cut(s) 268, 1121, 1151
BstKTI GATC 6 cut(s) 39, 501, 953, 1021, 1026, 1519
BstMAI GTCTC 3 cut(s) 525, 718, 1119
BstMBI GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
BstNI CCWGG 5 cut(s) 260, 543, 988, 1221, 1569
BstNSI RCATGY 4 cut(s) 296, 826, 848, 1582
BstSCI CCNGG 7 cut(s) 258, 541, 787, 788, 986, 1219, 1567
BstV1I GCAGC 1 cut(s) 583
BstX2I RGATCY 2 cut(s) 498, 1023
BstYI RGATCY 2 cut(s) 498, 1023
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 2 cut(s) 390, 943
BtsCI GGATG 3 cut(s) 268, 1121, 1151
BtsIMutI CAGTG 2 cut(s) 593, 723
Cac8I GCNNGC 4 cut(s) 143, 294, 1303, 1584
Cfr10I RCCGGY 2 cut(s) 141, 446
Cfr13I GGNCC 5 cut(s) 99, 203, 728, 884, 940
Cfr9I CCCGGG 1 cut(s) 788
CseI GACGC 1 cut(s) 700
CsiI ACCWGGT 1 cut(s) 541
Csp6I GTAC 3 cut(s) 991, 1102, 1241
CviQI GTAC 3 cut(s) 991, 1102, 1241
DdeI CTNAG 7 cut(s) 27, 158, 526, 715, 849, 1561, 1590
DpnI GATC 6 cut(s) 38, 500, 952, 1020, 1025, 1518
DpnII GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
DraI TTTAAA 1 cut(s) 670
DrdI GACNNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 1061
EaeI YGGCCR 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 1355
Eco130I CCWWGG 4 cut(s) 390, 691, 724, 943
Eco24I GRGCYC 1 cut(s) 1357
Eco31I GGTCTC 1 cut(s) 1119
Eco47I GGWCC 5 cut(s) 99, 203, 728, 884, 940
Eco53kI GAGCTC 1 cut(s) 1355
Eco57I CTGAAG 2 cut(s) 131, 347
Eco88I CYCGRG 5 cut(s) 297, 788, 1158, 1271, 1451
EcoICRI GAGCTC 1 cut(s) 1355
EcoO109I RGGNCCY 2 cut(s) 99, 203
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 5 cut(s) 258, 541, 986, 1219, 1567
EcoT14I CCWWGG 4 cut(s) 390, 691, 724, 943
EcoT38I GRGCYC 1 cut(s) 1357
ErhI CCWWGG 4 cut(s) 390, 691, 724, 943
Esp3I CGTCTC 1 cut(s) 718
FalI AAGNNNNNCTT 4 cut(s) 435, 467, 1370, 1402
FaqI GGGAC 5 cut(s) 69, 520, 870, 1067, 1254
FauNDI CATATG 2 cut(s) 967, 1072
Fnu4HI GCNGC 2 cut(s) 572, 1587
FokI GGATG 3 cut(s) 275, 1108, 1138
FriOI GRGCYC 1 cut(s) 1357
Fsp4HI GCNGC 2 cut(s) 572, 1587
FspBI CTAG 4 cut(s) 725, 1266, 1358, 1520
GluI GCNGC 2 cut(s) 572, 1587
HaeIII GGCC 1 cut(s) 141
HapII CCGG 5 cut(s) 142, 447, 789, 858, 917
HgaI GACGC 1 cut(s) 700
Hin1I GRCGYC 1 cut(s) 711
HincII GTYRAC 2 cut(s) 1131, 1558
HindII GTYRAC 2 cut(s) 1131, 1558
HindIII AAGCTT 4 cut(s) 109, 195, 664, 672
HpaII CCGG 5 cut(s) 142, 447, 789, 858, 917
Hpy166II GTNNAC 5 cut(s) 153, 646, 1131, 1416, 1558
Hpy188III TCNNGA 6 cut(s) 452, 1158, 1160, 1271, 1350, 1473
Hpy8I GTNNAC 5 cut(s) 153, 646, 1131, 1416, 1558
HpyAV CCTTC 4 cut(s) 36, 216, 762, 1473
HpyCH4IV ACGT 2 cut(s) 775, 1239
HpyCH4V TGCA 8 cut(s) 266, 279, 1181, 1193, 1206, 1305, 1507, 1582
HpyF3I CTNAG 7 cut(s) 27, 158, 526, 715, 849, 1561, 1590
HpySE526I ACGT 2 cut(s) 775, 1239
Hsp92I GRCGYC 1 cut(s) 711
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Kzo9I GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
LmnI GCTCC 4 cut(s) 352, 901, 1352, 1433
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 2 cut(s) 253, 883
MabI ACCWGGT 1 cut(s) 541
MaeI CTAG 4 cut(s) 725, 1266, 1358, 1520
MaeII ACGT 2 cut(s) 775, 1239
MaeIII GTNAC 6 cut(s) 340, 465, 912, 960, 1052, 1063
MalI GATC 6 cut(s) 38, 500, 952, 1020, 1025, 1518
MboI GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
MboII GAAGA 8 cut(s) 46, 508, 925, 970, 1032, 1393, 1435, 1544
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 2 cut(s) 498, 1023
MhlI GDGCHC 1 cut(s) 1357
MlyI GAGTC 5 cut(s) 542, 625, 914, 968, 1283
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 5 cut(s) 90, 844, 1095, 1165, 1355
MroNI GCCGGC 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 180, 1548
MseI TTAA 6 cut(s) 192, 236, 621, 669, 837, 1491
MspI CCGG 5 cut(s) 142, 447, 789, 858, 917
MspR9I CCNGG 7 cut(s) 260, 543, 789, 790, 988, 1221, 1569
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 5 cut(s) 260, 543, 988, 1221, 1569
NaeI GCCGGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 789, 790
NcoI CCATGG 2 cut(s) 390, 943
NdeI CATATG 2 cut(s) 967, 1072
NdeII GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
NgoMIV GCCGGC 1 cut(s) 141
NlaIV GGNNCC 5 cut(s) 101, 204, 697, 886, 1025
NmuCI GTSAC 6 cut(s) 340, 465, 912, 960, 1052, 1063
NspI RCATGY 4 cut(s) 296, 826, 848, 1582
NspV TTCGAA 1 cut(s) 19
PaeI GCATGC 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 1158
PdiI GCCGGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 180, 1548
PfeI GAWTC 6 cut(s) 176, 637, 1256, 1476, 1544, 1572
PkrI GCNGC 2 cut(s) 573, 1588
PleI GAGTC 5 cut(s) 541, 625, 914, 967, 1282
PpsI GAGTC 5 cut(s) 541, 625, 914, 967, 1282
PpuMI RGGWCCY 2 cut(s) 99, 203
PsiI TTATAA 1 cut(s) 1146
Psp124BI GAGCTC 1 cut(s) 1357
Psp5II RGGWCCY 2 cut(s) 99, 203
Psp6I CCWGG 5 cut(s) 258, 541, 986, 1219, 1567
PspGI CCWGG 5 cut(s) 258, 541, 986, 1219, 1567
PspN4I GGNNCC 5 cut(s) 101, 204, 697, 886, 1025
PspPI GGNCC 5 cut(s) 99, 203, 728, 884, 940
PspPPI RGGWCCY 2 cut(s) 99, 203
PsuI RGATCY 2 cut(s) 498, 1023
RsaI GTAC 3 cut(s) 992, 1103, 1242
RsaNI GTAC 3 cut(s) 991, 1102, 1241
SacI GAGCTC 1 cut(s) 1357
SaqAI TTAA 6 cut(s) 192, 236, 621, 669, 837, 1491
SatI GCNGC 2 cut(s) 572, 1587
Sau3AI GATC 6 cut(s) 36, 498, 950, 1018, 1023, 1516
Sau96I GGNCC 5 cut(s) 99, 203, 728, 884, 940
SchI GAGTC 5 cut(s) 542, 625, 914, 968, 1283
ScrFI CCNGG 7 cut(s) 260, 543, 789, 790, 988, 1221, 1569
SduI GDGCHC 1 cut(s) 1357
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 2 cut(s) 253, 883
Sfr274I CTCGAG 1 cut(s) 1158
SfuI TTCGAA 1 cut(s) 19
SinI GGWCC 5 cut(s) 99, 203, 728, 884, 940
SlaI CTCGAG 1 cut(s) 1158
SmaI CCCGGG 1 cut(s) 790
SmlI CTYRAG 2 cut(s) 452, 1158
SmoI CTYRAG 2 cut(s) 452, 1158
SphI GCATGC 1 cut(s) 296
SsiI CCGC 3 cut(s) 308, 882, 1587
SspMI CTAG 4 cut(s) 725, 1266, 1358, 1520
SstI GAGCTC 1 cut(s) 1357
StyD4I CCNGG 7 cut(s) 258, 541, 787, 788, 986, 1219, 1567
StyI CCWWGG 4 cut(s) 390, 691, 724, 943
TaiI ACGT 2 cut(s) 778, 1242
TaqI TCGA 5 cut(s) 19, 184, 224, 494, 1159
TauI GCSGC 1 cut(s) 1589
TfiI GAWTC 6 cut(s) 176, 637, 1256, 1476, 1544, 1572
Tru1I TTAA 6 cut(s) 192, 236, 621, 669, 837, 1491
Tru9I TTAA 6 cut(s) 192, 236, 621, 669, 837, 1491
TscAI CASTG 2 cut(s) 600, 723
TseFI GTSAC 6 cut(s) 340, 465, 912, 960, 1052, 1063
TseI GCWGC 1 cut(s) 571
Tsp45I GTSAC 6 cut(s) 340, 465, 912, 960, 1052, 1063
TspDTI ATGAA 5 cut(s) 449, 831, 1185, 1455, 1541
TspGWI ACGGA 1 cut(s) 1329
TspMI CCCGGG 1 cut(s) 788
TspRI CASTG 2 cut(s) 600, 723
VpaK11BI GGWCC 5 cut(s) 99, 203, 728, 884, 940
XapI RAATTY 3 cut(s) 783, 800, 1323
XceI RCATGY 4 cut(s) 296, 826, 848, 1582
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 1158
XmaI CCCGGG 1 cut(s) 788
XmaJI CCTAGG 1 cut(s) 724
XmnI GAANNNNTTC 2 cut(s) 180, 1548
XspI CTAG 4 cut(s) 725, 1266, 1358, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.