FvH4_4g17180

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013209
Physical Location & Seq
Reverse (-)
21070646 .. 21074063
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17180.t1

Sequence Viewer

Length: 1593 bp
ATGGAAGTTTCATTGTTTCAGAGCTCTCAGGTGAAGATTGGAAGAGCTGAATTGGGAAATAGGGAGTTTGGGTTTTGTAAACTGAGTGGTGATTTGAGGACCCAGATAAGCTTTGGTCGAAAAACCAGCTGGAAAAACGGCCGGGTTCAGTTTACTCTCCGGGCGGTTCAGTCTGAATCGATTCGACCCGTTAAAGTTCCGGGTCGTGTTAAAAGATCCAATTCGAATGATGGGGTGAGACTATTGGTTGGGTTGCCGCTGGATGCAGTTTCAGACTGCAATTCGGTGAACCATGCCCGAGCAATCGCGGCTGGACTAAAGGCTCTGAAACTATTGGGAGTGACCGGCGTGGAGCTTCCGGTGTGGTGGGGAGTTGTTGAGAAAGATGCCATGGGGAAGTATGAGTGGTCAGCCTACCATTCTCTAGTAGAGATGGTTCAGAAAGCCGGTCTTGAGGTTCATGTATCACTCTGCTTCCACGCTTCTAATCAACTGAAGATCTCACTCCCTGATTGGGTGTCTAGCTTAGGGGAGTCCCAACCTGGTATCTTCTTCAAAGACAGGTCAGGGCAGCAATACAAGGAGTGCTTATCACTGGCTGTTGATGAACTTCCTGTTCTCAATGGAAAGACTCCGATTCATGTTTACCGTGATTTCTGTGAAAGCTTTAAAGCTTCGTTCTCGCCTTTCCTCGGTTCCACAATCACGGGCATCTCGGTGAGCCTTGGACCAGATGGCGAGCTTCGATATCCTTCTCATCACCAGTCAGTCAAAAGAGGCAAAATTCCTGGGGTAGGTGAATTCCAGTGTTTTGATGAAAACATGCTTAGCGGTCTTAAACAACATGCTGAAGCAACCGGAAATCCTTTGTGGGGTCTTGGCGGTCCCCATGATGCTCCAAGCTATGACCAGTCACCGTACTCCAATGCCTTCTTCAAGGACCATGGGGGATCATGGGAGTCACCATATGGTGATTTCTTCCTGTCCTGGTACTCAAACCAGCTAATATCTCATGGAGATCGGATCCTTTCCCTTGCTTCTTCGACTTTTGGTGAAACAGAAGTCACAGTATATGGCAAAGTCCCTCTAATGTACTCTTGGTACAAAACAAGGTCTCACCCATCCGAGCTGACATCTGGGTTCTATAATACATCCTCTAGAGATGGTTATGAAGCAGTTGCAGATATGTTTGGAAGAAATTCCTGCAAAATGATATTGCCGGGATTAGACTTATCAGACGTACATCAACTGCATGAATCCCATTCTAGTCCCGAGTCATTACTGTCCCAAATTATAATGGTTTGCAGAAAGCACAGGGTCGAAATTTCTGGTCAAAATTCTTCAGTTTCAGGAGCTCCAGGAGGCTTTCAACAGATAAAGAAGAATTTGTTAGGTGAGAATGGGATAGATTTGTTCACATACCAGAGAATGGGAGCATATTTCTTCTCACCTGAGCATTTTCCTTCATTTGCTGGATTTGTACGAAGCCTTAATCAACTAGAATTGCAGTCAGATGATCTACCTGGTGAAGATGAAGCTACTGAATCTATTCATTCCAACTCAGAAGCAGGAATCCACATGCAAGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

531

Amino Acids

58.41

Weight (kDa)

6.26

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 83 - 482 1e-93 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1295
AccB7I CCANNNNNTGG 1 cut(s) 1429
AccII CGCG 1 cut(s) 308
AciI CCGC 6 cut(s) 164, 257, 308, 831, 882, 1587
AclWI GGATC 4 cut(s) 210, 958, 1018, 1031
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 6 cut(s) 783, 800, 1198, 1323, 1336, 1384
AcuI CTGAAG 3 cut(s) 515, 870, 1326
AfaI GTAC 6 cut(s) 920, 992, 1094, 1103, 1242, 1482
AfiI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 1429
AgsI TTSAA 3 cut(s) 556, 937, 1370
AjnI CCWGG 5 cut(s) 541, 787, 986, 1357, 1522
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
Alw21I GWGCWC 2 cut(s) 26, 1357
Alw26I GTCTC 2 cut(s) 232, 1119
AlwI GGATC 4 cut(s) 210, 958, 1018, 1031
Ama87I CYCGRG 2 cut(s) 297, 1271
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 571
ApoI RAATTY 6 cut(s) 783, 800, 1198, 1323, 1336, 1384
Asp700I GAANNNNTTC 3 cut(s) 180, 1198, 1548
AspS9I GGNCC 4 cut(s) 99, 728, 884, 940
AsuC2I CCSGG 4 cut(s) 143, 161, 201, 1221
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 2 cut(s) 297, 1271
AvaII GGWCC 4 cut(s) 99, 728, 884, 940
BamHI GGATCC 1 cut(s) 1023
BanII GRGCYC 2 cut(s) 26, 1357
Bbv12I GWGCWC 2 cut(s) 26, 1357
BbvI GCAGC 1 cut(s) 583
BccI CCATC 5 cut(s) 224, 427, 728, 1129, 1157
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 5 cut(s) 543, 789, 988, 1359, 1524
BcnI CCSGG 4 cut(s) 143, 161, 201, 1221
BcoDI GTCTC 2 cut(s) 232, 1119
BfaI CTAG 5 cut(s) 425, 522, 1158, 1266, 1499
BglII AGATCT 1 cut(s) 498
BisI GCNGC 4 cut(s) 257, 309, 572, 1587
BlpI GCTNAGC 1 cut(s) 827
BlsI GCNGC 4 cut(s) 258, 310, 573, 1588
Bme1390I CCNGG 9 cut(s) 143, 161, 201, 543, 789, 988, 1221, 1359, 1524
Bme18I GGWCC 4 cut(s) 99, 728, 884, 940
BmeT110I CYCGRG 2 cut(s) 297, 1271
BmgT120I GGNCC 4 cut(s) 99, 728, 884, 940
BmiI GGNNCC 4 cut(s) 101, 697, 886, 1025
BmrFI CCNGG 9 cut(s) 143, 161, 201, 543, 789, 988, 1221, 1359, 1524
BmsI GCATC 4 cut(s) 253, 376, 720, 883
BpmI CTGGAG 1 cut(s) 1341
Bpu10I CCTNAGC 2 cut(s) 526, 1452
Bpu1102I GCTNAGC 1 cut(s) 827
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 4 cut(s) 143, 161, 201, 1221
Bsa29I ATCGAT 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 5 cut(s) 390, 691, 724, 788, 943
BsaWI WCCGGW 2 cut(s) 358, 857
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 1429
Bse118I RCCGGY 2 cut(s) 344, 446
Bse1I ACTGG 4 cut(s) 600, 763, 805, 910
BseBI CCWGG 5 cut(s) 543, 789, 988, 1359, 1524
BseCI ATCGAT 1 cut(s) 179
BseDI CCNNGG 5 cut(s) 390, 691, 724, 788, 943
BseGI GGATG 3 cut(s) 268, 1121, 1151
BseLI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 1429
BseMII CTCAG 4 cut(s) 41, 74, 1443, 1575
BseNI ACTGG 4 cut(s) 600, 763, 805, 910
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 583
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 2 cut(s) 26, 1357
BsiHKCI CYCGRG 2 cut(s) 297, 1271
BsiSI CCGG 8 cut(s) 142, 160, 200, 345, 359, 447, 858, 1220
BslFI GGGAC 5 cut(s) 520, 870, 1067, 1254, 1270
BslI CCNNNNNNNGG 5 cut(s) 514, 692, 794, 872, 1429
BsmAI GTCTC 2 cut(s) 232, 1119
BsmFI GGGAC 5 cut(s) 520, 870, 1067, 1254, 1270
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 1119
BsoBI CYCGRG 2 cut(s) 297, 1271
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 26, 1357
Bsp143I GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
Bsp1720I GCTNAGC 1 cut(s) 827
Bsp19I CCATGG 2 cut(s) 390, 943
BspACI CCGC 6 cut(s) 164, 257, 308, 831, 882, 1587
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 4 cut(s) 40, 75, 1444, 1574
BspDI ATCGAT 1 cut(s) 179
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 4 cut(s) 101, 697, 886, 1025
BspPI GGATC 4 cut(s) 210, 958, 1018, 1031
BspQI GCTCTTC 1 cut(s) 37
BspT104I TTCGAA 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 1119
BsrFI RCCGGY 2 cut(s) 344, 446
BsrI ACTGG 4 cut(s) 600, 763, 805, 910
BssAI RCCGGY 2 cut(s) 344, 446
BssECI CCNNGG 5 cut(s) 390, 691, 724, 788, 943
BssMI GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
BssT1I CCWWGG 3 cut(s) 390, 724, 943
Bst2UI CCWGG 5 cut(s) 543, 789, 988, 1359, 1524
Bst4CI ACNGT 4 cut(s) 650, 918, 1069, 1284
Bst6I CTCTTC 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 740, 1584
BstDEI CTNAG 7 cut(s) 27, 83, 526, 827, 1452, 1561, 1590
BstDSI CCRYGG 2 cut(s) 390, 943
BstENI CCTNNNNNAGG 1 cut(s) 792
BstF5I GGATG 3 cut(s) 268, 1121, 1151
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 6 cut(s) 218, 501, 953, 1021, 1026, 1519
BstMAI GTCTC 2 cut(s) 232, 1119
BstMBI GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 5 cut(s) 543, 789, 988, 1359, 1524
BstNSI RCATGY 3 cut(s) 826, 848, 1582
BstSCI CCNGG 9 cut(s) 141, 159, 199, 541, 787, 986, 1219, 1357, 1522
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 583
BstX2I RGATCY 3 cut(s) 215, 498, 1023
BstYI RGATCY 3 cut(s) 215, 498, 1023
BstZI CGGCCG 1 cut(s) 139
Bsu15I ATCGAT 1 cut(s) 179
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 179
BtgI CCRYGG 2 cut(s) 390, 943
BtsCI GGATG 3 cut(s) 268, 1121, 1151
BtsIMutI CAGTG 2 cut(s) 593, 812
Cac8I GCNNGC 2 cut(s) 740, 1584
Cfr10I RCCGGY 2 cut(s) 344, 446
Cfr13I GGNCC 4 cut(s) 99, 728, 884, 940
ClaI ATCGAT 1 cut(s) 179
CsiI ACCWGGT 2 cut(s) 541, 1522
Csp6I GTAC 6 cut(s) 919, 991, 1093, 1102, 1241, 1481
CviQI GTAC 6 cut(s) 919, 991, 1093, 1102, 1241, 1481
DdeI CTNAG 7 cut(s) 27, 83, 526, 827, 1452, 1561, 1590
DpnI GATC 6 cut(s) 217, 500, 952, 1020, 1025, 1518
DpnII GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
DraI TTTAAA 1 cut(s) 670
EaeI YGGCCR 1 cut(s) 139
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Ecl136II GAGCTC 2 cut(s) 24, 1355
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 3 cut(s) 390, 724, 943
Eco24I GRGCYC 2 cut(s) 26, 1357
Eco31I GGTCTC 1 cut(s) 1119
Eco32I GATATC 1 cut(s) 749
Eco47I GGWCC 4 cut(s) 99, 728, 884, 940
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 24, 1355
Eco57I CTGAAG 3 cut(s) 515, 870, 1326
Eco88I CYCGRG 2 cut(s) 297, 1271
EcoICRI GAGCTC 2 cut(s) 24, 1355
EcoNI CCTNNNNNAGG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 99
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 5 cut(s) 541, 787, 986, 1357, 1522
EcoRV GATATC 1 cut(s) 749
EcoT14I CCWWGG 3 cut(s) 390, 724, 943
EcoT38I GRGCYC 2 cut(s) 26, 1357
ErhI CCWWGG 3 cut(s) 390, 724, 943
FalI AAGNNNNNCTT 4 cut(s) 435, 467, 572, 604
FaqI GGGAC 5 cut(s) 520, 870, 1067, 1254, 1270
FauNDI CATATG 1 cut(s) 967
Fnu4HI GCNGC 4 cut(s) 257, 309, 572, 1587
FokI GGATG 3 cut(s) 275, 1108, 1138
FriOI GRGCYC 2 cut(s) 26, 1357
Fsp4HI GCNGC 4 cut(s) 257, 309, 572, 1587
FspBI CTAG 5 cut(s) 425, 522, 1158, 1266, 1499
GluI GCNGC 4 cut(s) 257, 309, 572, 1587
GsuI CTGGAG 1 cut(s) 1341
HaeIII GGCC 1 cut(s) 141
HapII CCGG 8 cut(s) 142, 160, 200, 345, 359, 447, 858, 1220
HindIII AAGCTT 3 cut(s) 109, 664, 672
HpaII CCGG 8 cut(s) 142, 160, 200, 345, 359, 447, 858, 1220
Hpy166II GTNNAC 5 cut(s) 80, 153, 289, 646, 1416
Hpy188III TCNNGA 4 cut(s) 452, 1158, 1271, 1350
Hpy8I GTNNAC 5 cut(s) 80, 153, 289, 646, 1416
HpyAV CCTTC 3 cut(s) 762, 940, 1473
HpyCH4III ACNGT 4 cut(s) 650, 918, 1069, 1284
HpyCH4IV ACGT 1 cut(s) 1239
HpyCH4V TGCA 8 cut(s) 266, 279, 1181, 1206, 1252, 1305, 1507, 1582
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 7 cut(s) 27, 83, 526, 827, 1452, 1561, 1590
HpySE526I ACGT 1 cut(s) 1239
Kzo9I GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
LguI GCTCTTC 1 cut(s) 37
LmnI GCTCC 5 cut(s) 352, 901, 1352, 1360, 1433
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 4 cut(s) 253, 376, 720, 883
MabI ACCWGGT 2 cut(s) 541, 1522
MaeI CTAG 5 cut(s) 425, 522, 1158, 1266, 1499
MaeII ACGT 1 cut(s) 1239
MaeIII GTNAC 4 cut(s) 340, 912, 960, 1063
MalI GATC 6 cut(s) 217, 500, 952, 1020, 1025, 1518
MboI GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
MflI RGATCY 3 cut(s) 215, 498, 1023
MhlI GDGCHC 2 cut(s) 26, 1357
MlyI GAGTC 4 cut(s) 542, 625, 968, 1283
MmeI TCCRAC 1 cut(s) 1581
MnlI CCTC 7 cut(s) 90, 448, 701, 770, 1095, 1165, 1355
MroXI GAANNNNTTC 3 cut(s) 180, 1198, 1548
MseI TTAA 5 cut(s) 192, 210, 669, 837, 1491
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 129, 259
MspI CCGG 8 cut(s) 142, 160, 200, 345, 359, 447, 858, 1220
MspR9I CCNGG 9 cut(s) 143, 161, 201, 543, 789, 988, 1221, 1359, 1524
MvaI CCWGG 5 cut(s) 543, 789, 988, 1359, 1524
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 308
NciI CCSGG 4 cut(s) 143, 161, 201, 1221
NcoI CCATGG 2 cut(s) 390, 943
NdeI CATATG 1 cut(s) 967
NdeII GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
NlaIV GGNNCC 4 cut(s) 101, 697, 886, 1025
NmuCI GTSAC 4 cut(s) 340, 912, 960, 1063
NspI RCATGY 3 cut(s) 826, 848, 1582
NspV TTCGAA 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 37
PdmI GAANNNNTTC 3 cut(s) 180, 1198, 1548
PfeI GAWTC 6 cut(s) 176, 181, 637, 1256, 1544, 1572
PflMI CCANNNNNTGG 1 cut(s) 1429
PfoI TCCNGGA 1 cut(s) 1357
PkrI GCNGC 4 cut(s) 258, 310, 573, 1588
PleI GAGTC 4 cut(s) 541, 625, 967, 1282
PpsI GAGTC 4 cut(s) 541, 625, 967, 1282
PpuMI RGGWCCY 1 cut(s) 99
PsiI TTATAA 1 cut(s) 1295
Psp124BI GAGCTC 2 cut(s) 26, 1357
Psp5II RGGWCCY 1 cut(s) 99
Psp6I CCWGG 5 cut(s) 541, 787, 986, 1357, 1522
PspGI CCWGG 5 cut(s) 541, 787, 986, 1357, 1522
PspN4I GGNNCC 4 cut(s) 101, 697, 886, 1025
PspPI GGNCC 4 cut(s) 99, 728, 884, 940
PspPPI RGGWCCY 1 cut(s) 99
PsrI GAACNNNNNNTAC 2 cut(s) 420, 452
PsuI RGATCY 3 cut(s) 215, 498, 1023
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 6 cut(s) 920, 992, 1094, 1103, 1242, 1482
RsaNI GTAC 6 cut(s) 919, 991, 1093, 1102, 1241, 1481
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 2 cut(s) 26, 1357
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 5 cut(s) 192, 210, 669, 837, 1491
SatI GCNGC 4 cut(s) 257, 309, 572, 1587
Sau3AI GATC 6 cut(s) 215, 498, 950, 1018, 1023, 1516
Sau96I GGNCC 4 cut(s) 99, 728, 884, 940
SchI GAGTC 4 cut(s) 542, 625, 968, 1283
ScrFI CCNGG 9 cut(s) 143, 161, 201, 543, 789, 988, 1221, 1359, 1524
SduI GDGCHC 2 cut(s) 26, 1357
SexAI ACCWGGT 2 cut(s) 541, 1522
SfaNI GCATC 4 cut(s) 253, 376, 720, 883
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 4 cut(s) 99, 728, 884, 940
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SsiI CCGC 6 cut(s) 164, 257, 308, 831, 882, 1587
SspMI CTAG 5 cut(s) 425, 522, 1158, 1266, 1499
SstI GAGCTC 2 cut(s) 26, 1357
StyD4I CCNGG 9 cut(s) 141, 159, 199, 541, 787, 986, 1219, 1357, 1522
StyI CCWWGG 3 cut(s) 390, 724, 943
TaaI ACNGT 4 cut(s) 650, 918, 1069, 1284
TaiI ACGT 1 cut(s) 1242
TaqI TCGA 7 cut(s) 118, 179, 184, 224, 745, 1043, 1320
TatI WGTACW 1 cut(s) 1092
TauI GCSGC 3 cut(s) 259, 311, 1589
TfiI GAWTC 6 cut(s) 176, 181, 637, 1256, 1544, 1572
Tru1I TTAA 5 cut(s) 192, 210, 669, 837, 1491
Tru9I TTAA 5 cut(s) 192, 210, 669, 837, 1491
TscAI CASTG 2 cut(s) 600, 812
TseFI GTSAC 4 cut(s) 340, 912, 960, 1063
TseI GCWGC 1 cut(s) 571
Tsp45I GTSAC 4 cut(s) 340, 912, 960, 1063
TspDTI ATGAA 9 cut(s) 449, 621, 629, 831, 1185, 1269, 1455, 1541, 1548
TspRI CASTG 2 cut(s) 600, 812
Van91I CCANNNNNTGG 1 cut(s) 1429
VpaK11BI GGWCC 4 cut(s) 99, 728, 884, 940
XagI CCTNNNNNAGG 1 cut(s) 792
XapI RAATTY 6 cut(s) 783, 800, 1198, 1323, 1336, 1384
XbaI TCTAGA 1 cut(s) 1157
XceI RCATGY 3 cut(s) 826, 848, 1582
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmnI GAANNNNTTC 3 cut(s) 180, 1198, 1548
XspI CTAG 5 cut(s) 425, 522, 1158, 1266, 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.