Rmu_sc0015981.1_g000002

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0015981.1
OG0013209
Physical Location & Seq
Reverse (-)
12767 .. 14927
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0015981.1_g000002.1.cds

Sequence Viewer

Length: 1608 bp
atggaggtttctgtggttcgaagctctcgggtgaagatcggaagggctgaattggggcgcagggagttggggttttgtaaattgagtggtaatttgaggacccagataagctttggtcaaaacacgagctggaaaaatggccggcttcagtttactctcagggctgttcagtctgaatcggttcgacccgttacggttcgacccgttaaagcttcgggtccttccaaaagatccaattcgaatgacgggttaagactatttgttgggttgcccctggatgcagtttcagactgcaattcggtgaagcatgcccgagcaattgcggttggactaatggctctgaagctattgggagtgacaggcgtggagcttcctgtctggtggggaattgttgagaaagaagccatggggaagtatgagtggtcagcctaccattctttagcagagatggttcagaaagccggtcttgagcttcatgtgtcactctgcttccacgcttctaaacaaccgaagatctcactccccgattgggtgtctggcttaggggagtcccaacctggtatgtttttcaaagacaggtcagggcagcagttcaaggagtgtttatcactggctgttgatgagcttcctgttcttaatggaaagactccgattcaagtttaccatgatttctgcgaaagctttaaagcttcgttctcgactttccttggttccacaatcacgggcgtctcagtgagcctaggaccagatggggagcttcggtatccttctcatcaccagtcagtcaaacgtagcaaaattcccggggttggcgaattccaatgttttgatgaaaacatgctcagtgctcttaaacaacatgctgaggcaaccggaaatcctttatggggtcttggcggtccccatgatgctccgagctatgaccagtcaccggactcaaacactttcttcaaggaccatgggggatcatgggagtcaccatatggtgatttcttcctgtcctggtactcaaaccagctaatctctcatggagatcggatcctttcccttgcttcttcaactcttggtgacacagaagtcacagtatatggcaaagtccctctaatgcactcttggtacaaaacaaggtctcacccatccgagttgacatctgggttttataatacatcttctcgagatggttatgaagcagttgcagatatgtttgcaagaaactcctgcaaaatgatattgccgggactggacttatcagacgtacatcaaccacacgaatcccgttctagtcccgagtcattactatcacaaattagaacagcgtgcaaaaagcatggaattgaaatttctggtcaaaactcatcaatttcaggagctccaggaggctttcaacagataaagaagaacttgttaggagagaataaaatagacttgttcacttaccagagaatgggagcttatttcttctcacctgagcattttccttcgttttctggatttgtccgaagccttaatcaagtggaattgcagtcagacgatctagcttctgacgaagaagctactgaatctattcatgtcaactcagaaccaggaatccacatgcaagccgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

535

Amino Acids

58.82

Weight (kDa)

6.56

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AasI GACNNNNNNGTC 1 cut(s) 1076
AccB7I CCANNNNNTGG 1 cut(s) 1444
AciI CCGC 3 cut(s) 323, 897, 1602
AclWI GGATC 4 cut(s) 225, 973, 1033, 1046
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 3 cut(s) 798, 815, 1338
AcuI CTGAAG 2 cut(s) 131, 362
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 3 cut(s) 1007, 1118, 1257
AfiI CCNNNNNNNGG 5 cut(s) 529, 809, 887, 931, 1444
AgsI TTSAA 7 cut(s) 571, 595, 656, 952, 1059, 1337, 1385
AjnI CCWGG 5 cut(s) 273, 556, 1001, 1372, 1582
AloI GAACNNNNNNTCC 2 cut(s) 1572, 1604
Alw21I GWGCWC 2 cut(s) 850, 1372
Alw26I GTCTC 2 cut(s) 733, 1134
AlwI GGATC 4 cut(s) 225, 973, 1033, 1046
Ama87I CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 3 cut(s) 798, 815, 1338
Asp700I GAANNNNTTC 2 cut(s) 180, 1563
AspA2I CCTAGG 1 cut(s) 739
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 5 cut(s) 99, 218, 743, 899, 955
AsuC2I CCSGG 3 cut(s) 804, 805, 1236
AsuHPI GGTGA 9 cut(s) 43, 313, 767, 921, 969, 998, 1079, 1124, 1455
AsuII TTCGAA 2 cut(s) 19, 239
AvaI CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
AvaII GGWCC 5 cut(s) 99, 218, 743, 899, 955
AvrII CCTAGG 1 cut(s) 739
BamHI GGATCC 1 cut(s) 1038
BanII GRGCYC 1 cut(s) 1372
BauI CACGAG 1 cut(s) 124
Bbv12I GWGCWC 2 cut(s) 850, 1372
BbvCI CCTCAGC 1 cut(s) 864
BbvI GCAGC 1 cut(s) 598
BccI CCATC 4 cut(s) 442, 743, 1144, 1172
BciT130I CCWGG 5 cut(s) 275, 558, 1003, 1374, 1584
BciVI GTATCC 1 cut(s) 774
BcnI CCSGG 3 cut(s) 804, 805, 1236
BcoDI GTCTC 2 cut(s) 733, 1134
BfaI CTAG 3 cut(s) 740, 1281, 1535
BfuI GTATCC 1 cut(s) 774
BglII AGATCT 1 cut(s) 513
BisI GCNGC 2 cut(s) 587, 1602
BlnI CCTAGG 1 cut(s) 739
BlsI GCNGC 2 cut(s) 588, 1603
Bme1390I CCNGG 8 cut(s) 275, 558, 804, 805, 1003, 1236, 1374, 1584
Bme18I GGWCC 5 cut(s) 99, 218, 743, 899, 955
BmeT110I CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
BmgT120I GGNCC 5 cut(s) 99, 218, 743, 899, 955
BmiI GGNNCC 5 cut(s) 101, 219, 712, 901, 1040
BmrFI CCNGG 8 cut(s) 275, 558, 804, 805, 1003, 1236, 1374, 1584
BmsI GCATC 2 cut(s) 268, 898
BpmI CTGGAG 1 cut(s) 1356
Bpu10I CCTNAGC 3 cut(s) 541, 864, 1467
Bpu14I TTCGAA 2 cut(s) 19, 239
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 3 cut(s) 804, 805, 1236
BsaHI GRCGYC 1 cut(s) 726
BsaI GGTCTC 1 cut(s) 1134
BsaJI CCNNGG 7 cut(s) 273, 405, 706, 739, 803, 804, 958
BsaWI WCCGGW 2 cut(s) 872, 931
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 746, 776
Bsc4I CCNNNNNNNGG 5 cut(s) 529, 809, 887, 931, 1444
Bse118I RCCGGY 2 cut(s) 141, 461
Bse1I ACTGG 4 cut(s) 615, 778, 925, 1245
BseBI CCWGG 5 cut(s) 275, 558, 1003, 1374, 1584
BseDI CCNNGG 7 cut(s) 273, 405, 706, 739, 803, 804, 958
BseGI GGATG 2 cut(s) 283, 1136
BseLI CCNNNNNNNGG 5 cut(s) 529, 809, 887, 931, 1444
BseMII CTCAG 6 cut(s) 172, 744, 855, 856, 1458, 1590
BseNI ACTGG 4 cut(s) 615, 778, 925, 1245
BseXI GCAGC 1 cut(s) 598
BshFI GGCC 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 850, 1372
BsiHKCI CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
BsiSI CCGG 6 cut(s) 142, 462, 804, 873, 932, 1235
BslFI GGGAC 5 cut(s) 535, 885, 1082, 1251, 1269
BslI CCNNNNNNNGG 5 cut(s) 529, 809, 887, 931, 1444
BsmAI GTCTC 2 cut(s) 733, 1134
BsmBI CGTCTC 1 cut(s) 733
BsmFI GGGAC 5 cut(s) 535, 885, 1082, 1251, 1269
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 1134
BsoBI CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
Bsp119I TTCGAA 2 cut(s) 19, 239
Bsp1286I GDGCHC 2 cut(s) 850, 1372
Bsp143I GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
Bsp19I CCATGG 2 cut(s) 405, 958
BspACI CCGC 3 cut(s) 323, 897, 1602
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 6 cut(s) 171, 743, 855, 856, 1459, 1589
BspLI GGNNCC 5 cut(s) 101, 219, 712, 901, 1040
BspPI GGATC 4 cut(s) 225, 973, 1033, 1046
BspT104I TTCGAA 2 cut(s) 19, 239
BspTNI GGTCTC 1 cut(s) 1134
BsrFI RCCGGY 2 cut(s) 141, 461
BsrI ACTGG 4 cut(s) 615, 778, 925, 1245
BssAI RCCGGY 2 cut(s) 141, 461
BssECI CCNNGG 7 cut(s) 273, 405, 706, 739, 803, 804, 958
BssMI GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
BssNI GRCGYC 1 cut(s) 726
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 4 cut(s) 405, 706, 739, 958
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 5 cut(s) 275, 558, 1003, 1374, 1584
Bst4CI ACNGT 2 cut(s) 196, 1084
BstACI GRCGYC 1 cut(s) 726
BstBI TTCGAA 2 cut(s) 19, 239
BstC8I GCNNGC 4 cut(s) 143, 309, 1318, 1599
BstDEI CTNAG 8 cut(s) 158, 541, 730, 842, 864, 1467, 1576, 1605
BstDSI CCRYGG 2 cut(s) 405, 958
BstF5I GGATG 2 cut(s) 283, 1136
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 7 cut(s) 39, 233, 516, 968, 1036, 1041, 1534
BstMAI GTCTC 2 cut(s) 733, 1134
BstMBI GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
BstNI CCWGG 5 cut(s) 275, 558, 1003, 1374, 1584
BstNSI RCATGY 4 cut(s) 311, 841, 863, 1597
BstSCI CCNGG 8 cut(s) 273, 556, 802, 803, 1001, 1234, 1372, 1582
BstV1I GCAGC 1 cut(s) 598
BstX2I RGATCY 3 cut(s) 230, 513, 1038
BstYI RGATCY 3 cut(s) 230, 513, 1038
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 2 cut(s) 405, 958
BtsCI GGATG 2 cut(s) 283, 1136
BtsIMutI CAGTG 3 cut(s) 608, 738, 850
Cac8I GCNNGC 4 cut(s) 143, 309, 1318, 1599
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 2 cut(s) 141, 461
Cfr13I GGNCC 5 cut(s) 99, 218, 743, 899, 955
Cfr9I CCCGGG 1 cut(s) 803
CseI GACGC 1 cut(s) 715
CsiI ACCWGGT 1 cut(s) 556
Csp6I GTAC 3 cut(s) 1006, 1117, 1256
CviQI GTAC 3 cut(s) 1006, 1117, 1256
DdeI CTNAG 8 cut(s) 158, 541, 730, 842, 864, 1467, 1576, 1605
DpnI GATC 7 cut(s) 38, 232, 515, 967, 1035, 1040, 1533
DpnII GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
DraI TTTAAA 1 cut(s) 685
DrdI GACNNNNNNGTC 1 cut(s) 1076
DseDI GACNNNNNNGTC 1 cut(s) 1076
EaeI YGGCCR 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 1370
Eco130I CCWWGG 4 cut(s) 405, 706, 739, 958
Eco24I GRGCYC 1 cut(s) 1372
Eco31I GGTCTC 1 cut(s) 1134
Eco47I GGWCC 5 cut(s) 99, 218, 743, 899, 955
Eco53kI GAGCTC 1 cut(s) 1370
Eco57I CTGAAG 2 cut(s) 131, 362
Eco88I CYCGRG 5 cut(s) 27, 312, 803, 1173, 1286
EcoICRI GAGCTC 1 cut(s) 1370
EcoO109I RGGNCCY 2 cut(s) 99, 218
EcoRI GAATTC 1 cut(s) 815
EcoRII CCWGG 5 cut(s) 273, 556, 1001, 1372, 1582
EcoT14I CCWWGG 4 cut(s) 405, 706, 739, 958
EcoT38I GRGCYC 1 cut(s) 1372
ErhI CCWWGG 4 cut(s) 405, 706, 739, 958
Esp3I CGTCTC 1 cut(s) 733
FalI AAGNNNNNCTT 4 cut(s) 450, 482, 1385, 1417
FaqI GGGAC 5 cut(s) 535, 885, 1082, 1251, 1269
FauNDI CATATG 1 cut(s) 982
Fnu4HI GCNGC 2 cut(s) 587, 1602
FokI GGATG 2 cut(s) 290, 1123
FriOI GRGCYC 1 cut(s) 1372
Fsp4HI GCNGC 2 cut(s) 587, 1602
FspBI CTAG 3 cut(s) 740, 1281, 1535
GlaI GCGC 1 cut(s) 59
GluI GCNGC 2 cut(s) 587, 1602
GsuI CTGGAG 1 cut(s) 1356
HaeIII GGCC 1 cut(s) 141
HapII CCGG 6 cut(s) 142, 462, 804, 873, 932, 1235
HgaI GACGC 1 cut(s) 715
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 726
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 1146, 1573
HindII GTYRAC 2 cut(s) 1146, 1573
HindIII AAGCTT 4 cut(s) 109, 210, 679, 687
HpaII CCGG 6 cut(s) 142, 462, 804, 873, 932, 1235
HphI GGTGA 9 cut(s) 43, 313, 767, 921, 969, 998, 1079, 1124, 1455
Hpy166II GTNNAC 5 cut(s) 153, 661, 1146, 1431, 1573
Hpy188III TCNNGA 7 cut(s) 467, 697, 1173, 1175, 1286, 1365, 1488
Hpy8I GTNNAC 5 cut(s) 153, 661, 1146, 1431, 1573
HpyAV CCTTC 4 cut(s) 36, 231, 777, 1488
HpyCH4III ACNGT 2 cut(s) 196, 1084
HpyCH4IV ACGT 2 cut(s) 790, 1254
HpyCH4V TGCA 9 cut(s) 281, 294, 1108, 1196, 1208, 1221, 1320, 1522, 1597
HpyF3I CTNAG 8 cut(s) 158, 541, 730, 842, 864, 1467, 1576, 1605
HpySE526I ACGT 2 cut(s) 790, 1254
Hsp92I GRCGYC 1 cut(s) 726
HspAI GCGC 1 cut(s) 58
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Kzo9I GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
LmnI GCTCC 6 cut(s) 367, 754, 916, 1367, 1375, 1448
Lsp1109I GCAGC 1 cut(s) 598
LweI GCATC 2 cut(s) 268, 898
MabI ACCWGGT 1 cut(s) 556
MaeI CTAG 3 cut(s) 740, 1281, 1535
MaeII ACGT 2 cut(s) 790, 1254
MaeIII GTNAC 7 cut(s) 190, 355, 480, 927, 975, 1067, 1078
MalI GATC 7 cut(s) 38, 232, 515, 967, 1035, 1040, 1533
MboI GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
MboII GAAGA 9 cut(s) 46, 523, 940, 985, 1047, 1161, 1408, 1450, 1559
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 3 cut(s) 230, 513, 1038
MhlI GDGCHC 2 cut(s) 850, 1372
MlyI GAGTC 5 cut(s) 557, 640, 929, 983, 1298
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 4 cut(s) 90, 859, 1110, 1370
MroNI GCCGGC 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 180, 1563
MseI TTAA 6 cut(s) 207, 251, 636, 684, 852, 1506
MspI CCGG 6 cut(s) 142, 462, 804, 873, 932, 1235
MspR9I CCNGG 8 cut(s) 275, 558, 804, 805, 1003, 1236, 1374, 1584
MunI CAATTG 1 cut(s) 318
MvaI CCWGG 5 cut(s) 275, 558, 1003, 1374, 1584
NaeI GCCGGC 1 cut(s) 143
NciI CCSGG 3 cut(s) 804, 805, 1236
NcoI CCATGG 2 cut(s) 405, 958
NdeI CATATG 1 cut(s) 982
NdeII GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
NgoMIV GCCGGC 1 cut(s) 141
NlaIV GGNNCC 5 cut(s) 101, 219, 712, 901, 1040
NmuCI GTSAC 6 cut(s) 355, 480, 927, 975, 1067, 1078
NspI RCATGY 4 cut(s) 311, 841, 863, 1597
NspV TTCGAA 2 cut(s) 19, 239
PaeI GCATGC 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 1173
PdiI GCCGGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 180, 1563
PfeI GAWTC 5 cut(s) 176, 652, 1271, 1559, 1587
PflMI CCANNNNNTGG 1 cut(s) 1444
PfoI TCCNGGA 1 cut(s) 1372
PkrI GCNGC 2 cut(s) 588, 1603
PleI GAGTC 5 cut(s) 556, 640, 929, 982, 1297
PpsI GAGTC 5 cut(s) 556, 640, 929, 982, 1297
PpuMI RGGWCCY 2 cut(s) 99, 218
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 1372
Psp5II RGGWCCY 2 cut(s) 99, 218
Psp6I CCWGG 5 cut(s) 273, 556, 1001, 1372, 1582
PspGI CCWGG 5 cut(s) 273, 556, 1001, 1372, 1582
PspN4I GGNNCC 5 cut(s) 101, 219, 712, 901, 1040
PspPI GGNCC 5 cut(s) 99, 218, 743, 899, 955
PspPPI RGGWCCY 2 cut(s) 99, 218
PsuI RGATCY 3 cut(s) 230, 513, 1038
RsaI GTAC 3 cut(s) 1007, 1118, 1257
RsaNI GTAC 3 cut(s) 1006, 1117, 1256
SacI GAGCTC 1 cut(s) 1372
SaqAI TTAA 6 cut(s) 207, 251, 636, 684, 852, 1506
SatI GCNGC 2 cut(s) 587, 1602
Sau3AI GATC 7 cut(s) 36, 230, 513, 965, 1033, 1038, 1531
Sau96I GGNCC 5 cut(s) 99, 218, 743, 899, 955
SchI GAGTC 5 cut(s) 557, 640, 929, 983, 1298
ScrFI CCNGG 8 cut(s) 275, 558, 804, 805, 1003, 1236, 1374, 1584
SduI GDGCHC 2 cut(s) 850, 1372
SexAI ACCWGGT 1 cut(s) 556
SfaNI GCATC 2 cut(s) 268, 898
Sfr274I CTCGAG 1 cut(s) 1173
SfuI TTCGAA 2 cut(s) 19, 239
SinI GGWCC 5 cut(s) 99, 218, 743, 899, 955
SlaI CTCGAG 1 cut(s) 1173
SmaI CCCGGG 1 cut(s) 805
SmlI CTYRAG 2 cut(s) 467, 1173
SmoI CTYRAG 2 cut(s) 467, 1173
SphI GCATGC 1 cut(s) 311
SsiI CCGC 3 cut(s) 323, 897, 1602
SspMI CTAG 3 cut(s) 740, 1281, 1535
SstI GAGCTC 1 cut(s) 1372
StyD4I CCNGG 8 cut(s) 273, 556, 802, 803, 1001, 1234, 1372, 1582
StyI CCWWGG 4 cut(s) 405, 706, 739, 958
TaaI ACNGT 2 cut(s) 196, 1084
TaiI ACGT 2 cut(s) 793, 1257
TaqI TCGA 6 cut(s) 19, 184, 199, 239, 698, 1174
TauI GCSGC 1 cut(s) 1604
TfiI GAWTC 5 cut(s) 176, 652, 1271, 1559, 1587
Tru1I TTAA 6 cut(s) 207, 251, 636, 684, 852, 1506
Tru9I TTAA 6 cut(s) 207, 251, 636, 684, 852, 1506
TscAI CASTG 3 cut(s) 615, 738, 850
TseFI GTSAC 6 cut(s) 355, 480, 927, 975, 1067, 1078
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 6 cut(s) 355, 480, 927, 975, 1067, 1078
TspDTI ATGAA 4 cut(s) 464, 846, 1200, 1556
TspMI CCCGGG 1 cut(s) 803
TspRI CASTG 3 cut(s) 615, 738, 850
Van91I CCANNNNNTGG 1 cut(s) 1444
VpaK11BI GGWCC 5 cut(s) 99, 218, 743, 899, 955
XapI RAATTY 3 cut(s) 798, 815, 1338
XceI RCATGY 4 cut(s) 311, 841, 863, 1597
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 1173
XmaI CCCGGG 1 cut(s) 803
XmaJI CCTAGG 1 cut(s) 739
XmnI GAANNNNTTC 2 cut(s) 180, 1563
XspI CTAG 3 cut(s) 740, 1281, 1535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.