MD16G1175300.v1.1

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013209
Physical Location & Seq
Forward (+)
14880778 .. 14883822
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1175300.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGAGGTTTCGGTGTTTCGCGGCTCTCAGGCGGCGGTCAGAAAGGCGGAGTTGGTGCGAACGGAGTTTGCGTTTTCTAAGTTGAATGGAAATTTGAAGACCAACGTCTGTTTTGGTCAGAGCAAGAGCTGGAAAAGCGCCCGGCTTCAGTTTACTGTGAGGGCCGTACAATCCGATTCACCAGTCCGGTCCGATAAGGTTTCGGGTCCTGCCAAAAGATCCAAACCGAATGATGGAGTAAGATTATTTGTTGGGTTACCTCTAGATACAGTTTCAGACTGCAACGCGGTGAATCATGCTAGAGCGATTGCTGCCGGATTAAAGGCTCTGAAGTTGTTGGGGGTGGACGGTGTGGAGCTCCCGGTCTGGTGGGGAGTGGTTGAGAAAGAAGCCATGGGGAAGTACGAGTGGTCAGGCTACCTAGCCGTTGCAGAGATGGTTCAGAAAGCCGGCCTTGAGCTCCATGTCTCGCTCTGCTTCCATGCGTCTAAACAACCGAAGATCCCACTCCCTGCGTGGGTATCTCGCCTTGGTGAGTCCCAACCTGGTCTATTCTTTAAGGACCGGTCAGGGCAGCTCTACAAAGAGTGTTTATCGCTTGCTGTCGATGAACTTCCTGTTCTGAATGGAAAGACTCCAATCCAAGTTTACGAAGACTTCTGCGAAAGCTTTAAATCTTCGTTGGCGCCTTTCCTTGGTTCCACAATCACGGGCATCTCAATGAGCTTAGGACCAGATGGTGAGCTTCAATATCCTTCTCAGCACCGACTGGTCAAAAATAAAACTCCCGGAGTTGGAGAATTCCAGTGTTATGATGAAAACATGCTAAGGATTCTTAAGCAACATGCTGAAGCAGCCGGAAACCCTCTGTGGGGTCTTGGTGGTCCTCATGATGTTCCTAGCTACGACCAGTCACCAAATGCAAACAACTTCTTCAAGGATAATGGCGGTTCATGGGAGTCTCCATATGGTGACTTCTTCCTCTCCTGGTACTCGAACCAGCTCATTTCTCATGGAGATCGCCTCCTCTCTCTTGCCTCGTCAACTTTCGGTGACACTGAAGTGGAAGTTTGTGGTAAAGTTCCACTAATGCACTCTTGGTATAAAACAAGGGCTCACCCTTCTGAGCTGACTTCTGGGTTCTACAACACATCCTCTAGAGATGGTTACCAAGCAGTTGCTGAGATGTTTGCGAGGAACTCGTGCAAAATTATATTGCCTGGAATGGACCTATCAGATGAACATCAGCCACGGGACTCCCTTTCAAGTCCCGAGTTATTACTTTCACAAATTAAAACAGCTTGCAGAAAGCATGGGATTGAGATTGCTGGCCAAAACTCATCAGTTATGGGAGCTCGCGGAGGCTTTCAACAGATAAAGAAGAATTTGTTGGGAGAGAATGTGATCAACCTATTCACATATCAGAGAATGGGAGCGGACTTCTTCTCACCTGAACATTTTCCTTCATTTTCTGAGTTTGTTCGGAGCCTTAATCAACCACAACTGGAGTCGGATGATCTACCTACTGAAGAAGAAGCTGCTGAGTCCATACCTACAAATTCGGAATCAGTCATCCGCTTGCAAACCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

531

Amino Acids

58.38

Weight (kDa)

6.1

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 83 - 482 1.9e-95 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 685
AccBSI CCGCTC 1 cut(s) 1436
AccII CGCG 3 cut(s) 21, 287, 1359
AclWI GGATC 2 cut(s) 213, 496
AcoI YGGCCR 1 cut(s) 1330
AcsI RAATTY 4 cut(s) 91, 800, 1384, 1558
AcuI CTGAAG 5 cut(s) 131, 350, 870, 1080, 1548
AcyI GRCGYC 1 cut(s) 686
AfaI GTAC 3 cut(s) 168, 404, 992
AfiI CCNNNNNNNGG 6 cut(s) 233, 517, 695, 794, 871, 872
AflII CTTAAG 1 cut(s) 836
AgeI ACCGGT 1 cut(s) 564
AgsI TTSAA 6 cut(s) 85, 97, 749, 937, 1266, 1370
AjnI CCWGG 3 cut(s) 544, 986, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 1373, 1405
AleI CACNNNNGTG 1 cut(s) 1061
Alw21I GWGCWC 3 cut(s) 360, 462, 1357
Alw26I GTCTC 2 cut(s) 472, 966
AlwI GGATC 2 cut(s) 213, 496
AlwNI CAGNNNCTG 1 cut(s) 1181
Ama87I CYCGRG 1 cut(s) 1271
AoxI GGCC 3 cut(s) 162, 451, 1330
ApeKI GCWGC 4 cut(s) 311, 574, 854, 1538
ApoI RAATTY 4 cut(s) 91, 800, 1384, 1558
AsiGI ACCGGT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 1458
AspLEI GCGC 2 cut(s) 140, 688
AspS9I GGNCC 7 cut(s) 162, 189, 206, 562, 731, 884, 1228
AsuC2I CCSGG 3 cut(s) 142, 362, 789
AsuHPI GGTGA 9 cut(s) 171, 301, 545, 752, 906, 983, 1064, 1109, 1440
AvaI CYCGRG 1 cut(s) 1271
AvaII GGWCC 6 cut(s) 189, 206, 562, 731, 884, 1228
BalI TGGCCA 1 cut(s) 1332
BanI GGYRCC 1 cut(s) 685
BanII GRGCYC 4 cut(s) 360, 462, 1117, 1357
BauI CACGAG 1 cut(s) 1201
BbsI GAAGAC 2 cut(s) 104, 660
Bbv12I GWGCWC 3 cut(s) 360, 462, 1357
BbvI GCAGC 4 cut(s) 298, 586, 866, 1525
BccI CCATC 4 cut(s) 227, 430, 731, 1157
BceAI ACGGC 2 cut(s) 149, 410
BciT130I CCWGG 3 cut(s) 546, 988, 1221
BclI TGATCA 1 cut(s) 1404
BcnI CCSGG 3 cut(s) 142, 362, 789
BcoDI GTCTC 2 cut(s) 472, 966
BfaI CTAG 5 cut(s) 263, 300, 422, 900, 1158
BfoI RGCGCY 2 cut(s) 141, 689
BfrI CTTAAG 1 cut(s) 836
BisI GCNGC 6 cut(s) 22, 33, 312, 575, 855, 1539
BlsI GCNGC 6 cut(s) 23, 34, 313, 576, 856, 1540
Bme1390I CCNGG 6 cut(s) 142, 362, 546, 789, 988, 1221
Bme18I GGWCC 6 cut(s) 189, 206, 562, 731, 884, 1228
BmeT110I CYCGRG 1 cut(s) 1271
BmgT120I GGNCC 7 cut(s) 162, 189, 206, 562, 731, 884, 1228
BmiI GGNNCC 4 cut(s) 207, 687, 700, 1487
BmrFI CCNGG 6 cut(s) 142, 362, 546, 789, 988, 1221
BmsI GCATC 1 cut(s) 723
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 104, 660
BplI GAGNNNNNCTC 2 cut(s) 1008, 1040
BpmI CTGGAG 1 cut(s) 1526
Bpu10I CCTNAGC 2 cut(s) 727, 827
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 3 cut(s) 142, 362, 789
BsaBI GATNNNNATC 1 cut(s) 1242
BsaHI GRCGYC 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 393, 529, 694, 1250
BsaWI WCCGGW 2 cut(s) 186, 564
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 1386, 1416
Bsc4I CCNNNNNNNGG 6 cut(s) 233, 517, 695, 794, 871, 872
Bse118I RCCGGY 2 cut(s) 449, 564
Bse1I ACTGG 5 cut(s) 182, 774, 805, 910, 1509
Bse8I GATNNNNATC 1 cut(s) 1242
BseBI CCWGG 3 cut(s) 546, 988, 1221
BseDI CCNNGG 4 cut(s) 393, 529, 694, 1250
BseGI GGATG 3 cut(s) 1151, 1519, 1572
BseJI GATNNNNATC 1 cut(s) 1242
BseLI CCNNNNNNNGG 6 cut(s) 233, 517, 695, 794, 871, 872
BseMII CTCAG 6 cut(s) 41, 773, 1116, 1173, 1464, 1533
BseNI ACTGG 5 cut(s) 182, 774, 805, 910, 1509
BseRI GAGGAG 1 cut(s) 1016
BseXI GCAGC 4 cut(s) 298, 586, 866, 1525
Bsh1236I CGCG 3 cut(s) 21, 287, 1359
BshFI GGCC 3 cut(s) 164, 453, 1332
BshNI GGYRCC 1 cut(s) 685
BshTI ACCGGT 1 cut(s) 564
BsiHKAI GWGCWC 3 cut(s) 360, 462, 1357
BsiHKCI CYCGRG 1 cut(s) 1271
BsiSI CCGG 8 cut(s) 142, 187, 315, 362, 450, 565, 789, 858
BslFI GGGAC 3 cut(s) 523, 1254, 1268
BslI CCNNNNNNNGG 6 cut(s) 233, 517, 695, 794, 871, 872
BsmAI GTCTC 2 cut(s) 472, 966
BsmFI GGGAC 3 cut(s) 523, 1254, 1268
BsnI GGCC 3 cut(s) 164, 453, 1332
BsoBI CYCGRG 1 cut(s) 1271
Bsp1286I GDGCHC 4 cut(s) 360, 462, 1117, 1357
Bsp143I GATC 5 cut(s) 218, 501, 1018, 1404, 1516
Bsp19I CCATGG 1 cut(s) 393
BspANI GGCC 3 cut(s) 164, 453, 1332
BspCNI CTCAG 6 cut(s) 40, 772, 1117, 1174, 1465, 1534
BspFNI CGCG 3 cut(s) 21, 287, 1359
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 4 cut(s) 207, 687, 700, 1487
BspPI GGATC 2 cut(s) 213, 496
BspT107I GGYRCC 1 cut(s) 685
BspTI CTTAAG 1 cut(s) 836
BsrBI CCGCTC 1 cut(s) 1436
BsrFI RCCGGY 2 cut(s) 449, 564
BsrI ACTGG 5 cut(s) 182, 774, 805, 910, 1509
BssAI RCCGGY 2 cut(s) 449, 564
BssECI CCNNGG 4 cut(s) 393, 529, 694, 1250
BssMI GATC 5 cut(s) 218, 501, 1018, 1404, 1516
BssNI GRCGYC 1 cut(s) 686
BssSI CACGAG 1 cut(s) 1201
BssT1I CCWWGG 3 cut(s) 393, 529, 694
Bst2BI CACGAG 1 cut(s) 1201
Bst2UI CCWGG 3 cut(s) 546, 988, 1221
Bst4CI ACNGT 3 cut(s) 157, 271, 350
BstACI GRCGYC 1 cut(s) 686
BstAFI CTTAAG 1 cut(s) 836
BstC8I GCNNGC 6 cut(s) 451, 600, 1303, 1330, 1357, 1580
BstDEI CTNAG 9 cut(s) 27, 78, 727, 759, 827, 1125, 1182, 1473, 1542
BstDSI CCRYGG 2 cut(s) 393, 1250
BstEII GGTNACC 2 cut(s) 255, 1166
BstF5I GGATG 3 cut(s) 1151, 1519, 1572
BstFNI CGCG 3 cut(s) 21, 287, 1359
BstH2I RGCGCY 2 cut(s) 141, 689
BstHHI GCGC 2 cut(s) 140, 688
BstKTI GATC 5 cut(s) 221, 504, 1021, 1407, 1519
BstMAI GTCTC 2 cut(s) 472, 966
BstMBI GATC 5 cut(s) 218, 501, 1018, 1404, 1516
BstMWI GCNNNNNNNGC 3 cut(s) 135, 311, 854
BstNI CCWGG 3 cut(s) 546, 988, 1221
BstNSI RCATGY 2 cut(s) 826, 848
BstPAI GACNNNNGTC 1 cut(s) 104
BstPI GGTNACC 2 cut(s) 255, 1166
BstSCI CCNGG 6 cut(s) 140, 360, 544, 787, 986, 1219
BstUI CGCG 3 cut(s) 21, 287, 1359
BstV1I GCAGC 4 cut(s) 298, 586, 866, 1525
BstV2I GAAGAC 2 cut(s) 104, 660
BstX2I RGATCY 2 cut(s) 218, 501
BstYI RGATCY 2 cut(s) 218, 501
BsuRI GGCC 3 cut(s) 164, 453, 1332
BtgI CCRYGG 2 cut(s) 393, 1250
BtsCI GGATG 3 cut(s) 1151, 1519, 1572
BtsIMutI CAGTG 2 cut(s) 812, 1056
Cac8I GCNNGC 6 cut(s) 451, 600, 1303, 1330, 1357, 1580
CaiI CAGNNNCTG 1 cut(s) 1181
CciI TCATGA 1 cut(s) 889
CfoI GCGC 2 cut(s) 140, 688
Cfr10I RCCGGY 2 cut(s) 449, 564
Cfr13I GGNCC 7 cut(s) 162, 189, 206, 562, 731, 884, 1228
CpoI CGGWCCG 1 cut(s) 189
CseI GACGC 1 cut(s) 474
CsiI ACCWGGT 1 cut(s) 544
Csp6I GTAC 3 cut(s) 167, 403, 991
CspAI ACCGGT 1 cut(s) 564
CspI CGGWCCG 1 cut(s) 189
CviQI GTAC 3 cut(s) 167, 403, 991
DdeI CTNAG 9 cut(s) 27, 78, 727, 759, 827, 1125, 1182, 1473, 1542
DinI GGCGCC 1 cut(s) 687
DpnI GATC 5 cut(s) 220, 503, 1020, 1406, 1518
DpnII GATC 5 cut(s) 218, 501, 1018, 1404, 1516
DraI TTTAAA 1 cut(s) 673
EaeI YGGCCR 1 cut(s) 1330
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 3 cut(s) 358, 460, 1355
Eco130I CCWWGG 3 cut(s) 393, 529, 694
Eco24I GRGCYC 4 cut(s) 360, 462, 1117, 1357
Eco47I GGWCC 6 cut(s) 189, 206, 562, 731, 884, 1228
Eco53kI GAGCTC 3 cut(s) 358, 460, 1355
Eco57I CTGAAG 5 cut(s) 131, 350, 870, 1080, 1548
Eco88I CYCGRG 1 cut(s) 1271
Eco91I GGTNACC 2 cut(s) 255, 1166
EcoICRI GAGCTC 3 cut(s) 358, 460, 1355
EcoO109I RGGNCCY 1 cut(s) 206
EcoO65I GGTNACC 2 cut(s) 255, 1166
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 3 cut(s) 544, 986, 1219
EcoT14I CCWWGG 3 cut(s) 393, 529, 694
EcoT38I GRGCYC 4 cut(s) 360, 462, 1117, 1357
EgeI GGCGCC 1 cut(s) 687
EheI GGCGCC 1 cut(s) 687
ErhI CCWWGG 3 cut(s) 393, 529, 694
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 3 cut(s) 523, 1254, 1268
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 1 cut(s) 1404
Fnu4HI GCNGC 6 cut(s) 22, 33, 312, 575, 855, 1539
FokI GGATG 3 cut(s) 1138, 1526, 1559
FriOI GRGCYC 4 cut(s) 360, 462, 1117, 1357
Fsp4HI GCNGC 6 cut(s) 22, 33, 312, 575, 855, 1539
FspBI CTAG 5 cut(s) 263, 300, 422, 900, 1158
GlaI GCGC 2 cut(s) 139, 687
GluI GCNGC 6 cut(s) 22, 33, 312, 575, 855, 1539
GsuI CTGGAG 1 cut(s) 1526
HaeII RGCGCY 2 cut(s) 141, 689
HaeIII GGCC 3 cut(s) 164, 453, 1332
HapII CCGG 8 cut(s) 142, 187, 315, 362, 450, 565, 789, 858
HgaI GACGC 1 cut(s) 474
HhaI GCGC 2 cut(s) 140, 688
Hin1I GRCGYC 1 cut(s) 686
Hin6I GCGC 2 cut(s) 138, 686
HinP1I GCGC 2 cut(s) 138, 686
HincII GTYRAC 1 cut(s) 1044
HindII GTYRAC 1 cut(s) 1044
HindIII AAGCTT 1 cut(s) 667
HpaII CCGG 8 cut(s) 142, 187, 315, 362, 450, 565, 789, 858
HphI GGTGA 9 cut(s) 171, 301, 545, 752, 906, 983, 1064, 1109, 1440
Hpy166II GTNNAC 4 cut(s) 153, 346, 649, 1044
Hpy188III TCNNGA 4 cut(s) 263, 890, 1158, 1271
Hpy8I GTNNAC 4 cut(s) 153, 346, 649, 1044
HpyAV CCTTC 3 cut(s) 765, 1131, 1473
HpyCH4III ACNGT 3 cut(s) 157, 271, 350
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 7 cut(s) 282, 431, 923, 1093, 1206, 1305, 1582
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 311, 854
HpyF3I CTNAG 9 cut(s) 27, 78, 727, 759, 827, 1125, 1182, 1473, 1542
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 686
HspAI GCGC 2 cut(s) 138, 686
KasI GGCGCC 1 cut(s) 685
KroI GCCGGC 1 cut(s) 449
KroNI GCCGGC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 1404
Kzo9I GATC 5 cut(s) 218, 501, 1018, 1404, 1516
LmnI GCTCC 6 cut(s) 355, 363, 465, 1352, 1433, 1485
Lsp1109I GCAGC 4 cut(s) 298, 586, 866, 1525
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 544
MaeI CTAG 5 cut(s) 263, 300, 422, 900, 1158
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 5 cut(s) 255, 912, 971, 1052, 1166
MalI GATC 5 cut(s) 220, 503, 1020, 1406, 1518
MbiI CCGCTC 1 cut(s) 1436
MboI GATC 5 cut(s) 218, 501, 1018, 1404, 1516
MflI RGATCY 2 cut(s) 218, 501
MhlI GDGCHC 4 cut(s) 360, 462, 1117, 1357
MlsI TGGCCA 1 cut(s) 1332
MluCI AATT 6 cut(s) 91, 800, 1209, 1290, 1384, 1558
MluNI TGGCCA 1 cut(s) 1332
Mly113I GGCGCC 1 cut(s) 686
MlyI GAGTC 6 cut(s) 545, 628, 968, 1250, 1517, 1553
MmeI TCCRAC 2 cut(s) 775, 1491
Mox20I TGGCCA 1 cut(s) 1332
MroNI GCCGGC 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 1458
MscI TGGCCA 1 cut(s) 1332
MseI TTAA 7 cut(s) 320, 558, 672, 837, 1293, 1491, 1591
MslI CAYNNNNRTG 2 cut(s) 719, 1061
Msp20I TGGCCA 1 cut(s) 1332
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 8 cut(s) 142, 187, 315, 362, 450, 565, 789, 858
MspR9I CCNGG 6 cut(s) 142, 362, 546, 789, 988, 1221
MvaI CCWGG 3 cut(s) 546, 988, 1221
MvnI CGCG 3 cut(s) 21, 287, 1359
MwoI GCNNNNNNNGC 3 cut(s) 135, 311, 854
NaeI GCCGGC 1 cut(s) 451
NarI GGCGCC 1 cut(s) 686
NciI CCSGG 3 cut(s) 142, 362, 789
NcoI CCATGG 1 cut(s) 393
NdeI CATATG 1 cut(s) 967
NdeII GATC 5 cut(s) 218, 501, 1018, 1404, 1516
NgoMIV GCCGGC 1 cut(s) 449
NlaIV GGNNCC 4 cut(s) 207, 687, 700, 1487
NmuCI GTSAC 3 cut(s) 912, 971, 1052
NspI RCATGY 2 cut(s) 826, 848
OliI CACNNNNGTG 1 cut(s) 1061
PagI TCATGA 1 cut(s) 889
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 1458
PfeI GAWTC 4 cut(s) 176, 292, 832, 1565
PfoI TCCNGGA 1 cut(s) 787
PinAI ACCGGT 1 cut(s) 564
PkrI GCNGC 6 cut(s) 23, 34, 313, 576, 856, 1540
PleI GAGTC 6 cut(s) 544, 628, 967, 1250, 1516, 1552
PluTI GGCGCC 1 cut(s) 689
PpsI GAGTC 6 cut(s) 544, 628, 967, 1250, 1516, 1552
PpuMI RGGWCCY 1 cut(s) 206
PshAI GACNNNNGTC 1 cut(s) 104
Psp124BI GAGCTC 3 cut(s) 360, 462, 1357
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 3 cut(s) 544, 986, 1219
PspEI GGTNACC 2 cut(s) 255, 1166
PspGI CCWGG 3 cut(s) 544, 986, 1219
PspN4I GGNNCC 4 cut(s) 207, 687, 700, 1487
PspPI GGNCC 7 cut(s) 162, 189, 206, 562, 731, 884, 1228
PspPPI RGGWCCY 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 2 cut(s) 218, 501
RsaI GTAC 3 cut(s) 168, 404, 992
RsaNI GTAC 3 cut(s) 167, 403, 991
RseI CAYNNNNRTG 2 cut(s) 719, 1061
Rsr2I CGGWCCG 1 cut(s) 189
RsrII CGGWCCG 1 cut(s) 189
SacI GAGCTC 3 cut(s) 360, 462, 1357
SaqAI TTAA 7 cut(s) 320, 558, 672, 837, 1293, 1491, 1591
SatI GCNGC 6 cut(s) 22, 33, 312, 575, 855, 1539
Sau3AI GATC 5 cut(s) 218, 501, 1018, 1404, 1516
Sau96I GGNCC 7 cut(s) 162, 189, 206, 562, 731, 884, 1228
SchI GAGTC 6 cut(s) 545, 628, 968, 1250, 1517, 1553
ScrFI CCNGG 6 cut(s) 142, 362, 546, 789, 988, 1221
SduI GDGCHC 4 cut(s) 360, 462, 1117, 1357
SexAI ACCWGGT 1 cut(s) 544
SfaNI GCATC 1 cut(s) 723
SfoI GGCGCC 1 cut(s) 687
SinI GGWCC 6 cut(s) 189, 206, 562, 731, 884, 1228
SmiMI CAYNNNNRTG 2 cut(s) 719, 1061
SmlI CTYRAG 2 cut(s) 455, 836
SmoI CTYRAG 2 cut(s) 455, 836
Sse9I AATT 6 cut(s) 91, 800, 1209, 1290, 1384, 1558
SspDI GGCGCC 1 cut(s) 685
SspMI CTAG 5 cut(s) 263, 300, 422, 900, 1158
SstI GAGCTC 3 cut(s) 360, 462, 1357
StyD4I CCNGG 6 cut(s) 140, 360, 544, 787, 986, 1219
StyI CCWWGG 3 cut(s) 393, 529, 694
TaaI ACNGT 3 cut(s) 157, 271, 350
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 606, 995
TasI AATT 6 cut(s) 91, 800, 1209, 1290, 1384, 1558
TauI GCSGC 2 cut(s) 24, 35
TfiI GAWTC 4 cut(s) 176, 292, 832, 1565
Tru1I TTAA 7 cut(s) 320, 558, 672, 837, 1293, 1491, 1591
Tru9I TTAA 7 cut(s) 320, 558, 672, 837, 1293, 1491, 1591
TscAI CASTG 2 cut(s) 812, 1063
TseFI GTSAC 3 cut(s) 912, 971, 1052
TseI GCWGC 4 cut(s) 311, 574, 854, 1538
Tsp45I GTSAC 3 cut(s) 912, 971, 1052
TspDTI ATGAA 5 cut(s) 624, 831, 942, 1254, 1455
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 2 cut(s) 812, 1063
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 6 cut(s) 189, 206, 562, 731, 884, 1228
XapI RAATTY 4 cut(s) 91, 800, 1384, 1558
XbaI TCTAGA 2 cut(s) 262, 1157
XceI RCATGY 2 cut(s) 826, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 1458
XspI CTAG 5 cut(s) 263, 300, 422, 900, 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.