MD13G1174000.v1.1

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013209
Physical Location & Seq
Forward (+)
14338579 .. 14341143
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1174000.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGAGGTTTCGGTGTTTCGCGGCTCTCAGGCGGCAATCGGAAAGACCGAGTTGGAGCGAACGGAGCTTGGGTTTTGTGAATTGAACGGAAATTTGAAGACCAACGTCTGTTTTGGTCAGAGCACGAGCTGGAAAAATCCCCGGCTTCAGTTTACTGTGAGGGCCGTACAATCCGAAACGGTTCGGTCCGGTAAAGTTTCGGGACCTGCCAGAAAATCAAAACCGAACGATGGAGTGAGATTATTTGTTGGGCTACCTCTAGATACGGTTTCAGACTGCAACGCGGTGAACCATGCTAGAGCGATTGCGGCCGGATTGAAGGCTCTGAAGCTGTTGGGGGTGGACGGCGTTGAGCTCCCGGTCTGGTGGGGAATGGTTGAGAAAGAAGCCATGGGGAAGTATGAGTGGTCAGGCTATCTTGCCGTTGCGGAGATGGTTCAGAAAGCCGGCCTCGAGCTCCATGTCTCACTCTGCTTCCATGCTTCTAAACAACCCAAGATCCCACTCCCTGCGTGGGTATCTCGCCTTGGCGCGTCCCAGCCTAGTATCTTTTTCAAGGACCGGTCGGGGCAGCATTACAAAGAGTGCTTATCGCTTGCTGTGGATGAACTTCCTGTACTGAATGGAAAGACTCCAACCCAAGTTTACCAAGACTTCTGTGAAAGCTTTAAATCTTCGTTCGAGCCTTTCCTGGGTTCCACAATCGCGGGCATCTCAATGAGCTTAGGACCAGATGGCGAGCTTCAATATCCATCTCAGCGCCGGCTAGGCAAGAACAAGATTCCCGGGGTTGGAGAATTCCAATGTTATGATGAAAACATGCTTAGCATTCTTAAGCAACACGCTGAAGCAGCCGGAAACCCTTTGTGGGGTCTTGGTGGTCCTCATGATGTTCCTAGCTACGATCAGTCCCCAAACGCAAACAACTTCTTCAAGGATGATGGCGGTTCATGGGAGTCTCCATATGGTGATTTCTTCCTTTCCTGGTACTCAAACCAGCTTATTTCTCACGGAGACCGCCTCCTCGACCTTGTCTCTTCAACTTTCAGTGACACTGAAGTGGAAATTTGTGGCAAGGTCCCGCTAATGCACTCTTGGTACAAAACAAGGTCTCACCCTTCTGAGCTGACTTCTGGGTTCTATAACACATCCTCTAGAGATGGTTACCAAGCAGTTGCCCAGATGTTTGCAAGGAATTCGTGCAAAATTATATTGCCTGGAATGGACCTATCGGACGAACATCAGCCACAGGACTCCCTTTCGAGTCCCGAGTTATTACTATCACAAATTAAAACCGCTTGCAGAAAGCATGGGGTTGAGATTTCAGGCCAAAACTCATCAGTTTCGGGAGCTCGCGAAGGCTTTCAACAGATAAAGAAGAATTTGTTGGGAGAGAATGCAATCAACCTGTTCACTTATCAGAGAATGGGAGCTGATTTCTTCTCGCCCGATCATTTTCCTTCGTTTTCTGAGTTTGTTCGGAGCCTTAACCAACCGCAGCTGCAGTCAGACGATCTACCAATTGAAGAAGAAGCTGTTGAGTCCGTACCTACGAATTCGGAATCAGTCGTCCGCATGCAAACCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

530

Amino Acids

58.32

Weight (kDa)

5.64

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 82 - 481 1.6e-97 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18670
fragaria_vesca FvH4_4g17180 FvH4_4g17180
malus_domestica MD13G1174000.v1.1 MD16G1175300.v1.1
prunus_persica Prupe.1G142400_v2.0.a1
pyrus_communis pycom13g14970 pycom16g14700
rosa_chinensis RchiOBHm_Chr4g0420971
rosa_laevigata RLG00000007716
rosa_multiflora Rmu_sc0015981.1_g000002
rosa_roxburghii Rroxscaffold_5G00363330
rosa_rugosa Rorug04G0167500
rosa_samantha Rh4AG228600 Rh4BG231800 Rh4CG243500 Rh4DG227200
rosa_wichuraiana Rw4G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 214
AccII CGCG 5 cut(s) 21, 284, 533, 707, 1356
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 6 cut(s) 91, 797, 1065, 1195, 1381, 1555
AcuI CTGAAG 4 cut(s) 131, 347, 867, 1077
AfaI GTAC 5 cut(s) 168, 618, 989, 1100, 1548
AfiI CCNNNNNNNGG 6 cut(s) 230, 514, 692, 791, 868, 869
AflII CTTAAG 1 cut(s) 833
AgeI ACCGGT 1 cut(s) 561
AgsI TTSAA 9 cut(s) 85, 97, 319, 556, 746, 934, 1041, 1367, 1526
AjnI CCWGG 3 cut(s) 690, 983, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 1370, 1402
AleI CACNNNNGTG 1 cut(s) 1058
Alw21I GWGCWC 4 cut(s) 125, 357, 459, 1354
Alw26I GTCTC 5 cut(s) 469, 963, 1008, 1039, 1116
AlwI GGATC 1 cut(s) 493
Ama87I CYCGRG 3 cut(s) 452, 785, 1268
AoxI GGCC 4 cut(s) 162, 309, 448, 1327
ApeKI GCWGC 4 cut(s) 571, 851, 1498, 1501
ApoI RAATTY 6 cut(s) 91, 797, 1065, 1195, 1381, 1555
AsiGI ACCGGT 1 cut(s) 561
Asp700I GAANNNNTTC 2 cut(s) 180, 1362
AspLEI GCGC 2 cut(s) 533, 762
AspS9I GGNCC 8 cut(s) 162, 186, 203, 559, 728, 881, 1078, 1225
AsuC2I CCSGG 4 cut(s) 142, 359, 786, 787
AsuHPI GGTGA 3 cut(s) 298, 980, 1106
AvaI CYCGRG 3 cut(s) 452, 785, 1268
AvaII GGWCC 7 cut(s) 186, 203, 559, 728, 881, 1078, 1225
BanII GRGCYC 3 cut(s) 357, 459, 1354
BauI CACGAG 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 4 cut(s) 125, 357, 459, 1354
BbvI GCAGC 4 cut(s) 583, 863, 1488, 1510
BccI CCATC 6 cut(s) 224, 427, 728, 760, 935, 1154
BceAI ACGGC 3 cut(s) 149, 361, 407
BcgI CGANNNNNNTGC 2 cut(s) 1179, 1213
BciT130I CCWGG 3 cut(s) 692, 985, 1218
BcnI CCSGG 4 cut(s) 142, 359, 786, 787
BcoDI GTCTC 5 cut(s) 469, 963, 1008, 1039, 1116
BfaI CTAG 6 cut(s) 260, 297, 543, 767, 897, 1155
BfmI CTRYAG 1 cut(s) 1502
BfoI RGCGCY 1 cut(s) 763
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 768
BisI GCNGC 7 cut(s) 22, 33, 309, 572, 852, 1499, 1502
BlpI GCTNAGC 1 cut(s) 824
BlsI GCNGC 7 cut(s) 23, 34, 310, 573, 853, 1500, 1503
Bme1390I CCNGG 7 cut(s) 142, 359, 692, 786, 787, 985, 1218
Bme18I GGWCC 7 cut(s) 186, 203, 559, 728, 881, 1078, 1225
BmeT110I CYCGRG 3 cut(s) 452, 785, 1268
BmgT120I GGNCC 8 cut(s) 162, 186, 203, 559, 728, 881, 1078, 1225
BmiI GGNNCC 4 cut(s) 204, 697, 1080, 1484
BmrFI CCNGG 7 cut(s) 142, 359, 692, 786, 787, 985, 1218
BmsI GCATC 1 cut(s) 720
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 1005, 1037
Bpu10I CCTNAGC 1 cut(s) 724
Bpu1102I GCTNAGC 1 cut(s) 824
BpuMI CCSGG 4 cut(s) 142, 359, 786, 787
BsaI GGTCTC 2 cut(s) 1008, 1116
BsaJI CCNNGG 6 cut(s) 140, 390, 526, 691, 785, 786
BsaWI WCCGGW 2 cut(s) 188, 561
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 514, 692, 791, 868, 869
Bse118I RCCGGY 3 cut(s) 446, 561, 762
BseBI CCWGG 3 cut(s) 692, 985, 1218
BseDI CCNNGG 6 cut(s) 140, 390, 526, 691, 785, 786
BseGI GGATG 3 cut(s) 610, 943, 1148
BseLI CCNNNNNNNGG 6 cut(s) 230, 514, 692, 791, 868, 869
BseMII CTCAG 4 cut(s) 41, 770, 1113, 1461
BseRI GAGGAG 1 cut(s) 1013
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 4 cut(s) 583, 863, 1488, 1510
BseYI CCCAGC 1 cut(s) 537
Bsh1236I CGCG 5 cut(s) 21, 284, 533, 707, 1356
Bsh1285I CGRYCG 2 cut(s) 312, 566
BshFI GGCC 4 cut(s) 164, 311, 450, 1329
BshTI ACCGGT 1 cut(s) 561
BsiEI CGRYCG 2 cut(s) 312, 566
BsiHKAI GWGCWC 4 cut(s) 125, 357, 459, 1354
BsiHKCI CYCGRG 3 cut(s) 452, 785, 1268
BsiSI CCGG 9 cut(s) 142, 189, 312, 359, 447, 562, 763, 786, 855
BslFI GGGAC 5 cut(s) 216, 520, 895, 1064, 1251
BslI CCNNNNNNNGG 6 cut(s) 230, 514, 692, 791, 868, 869
BsmAI GTCTC 5 cut(s) 469, 963, 1008, 1039, 1116
BsmFI GGGAC 5 cut(s) 216, 520, 895, 1064, 1251
BsmI GAATGC 2 cut(s) 828, 1402
BsnI GGCC 4 cut(s) 164, 311, 450, 1329
Bso31I GGTCTC 2 cut(s) 1008, 1116
BsoBI CYCGRG 3 cut(s) 452, 785, 1268
Bsp1286I GDGCHC 4 cut(s) 125, 357, 459, 1354
Bsp143I GATC 4 cut(s) 498, 904, 1450, 1513
Bsp1720I GCTNAGC 1 cut(s) 824
Bsp19I CCATGG 1 cut(s) 390
Bsp68I TCGCGA 1 cut(s) 1356
BspANI GGCC 4 cut(s) 164, 311, 450, 1329
BspCNI CTCAG 4 cut(s) 40, 769, 1114, 1462
BspFNI CGCG 5 cut(s) 21, 284, 533, 707, 1356
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 4 cut(s) 204, 697, 1080, 1484
BspMAI CTGCAG 1 cut(s) 1506
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 1 cut(s) 493
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 2 cut(s) 1008, 1116
BsrFI RCCGGY 3 cut(s) 446, 561, 762
BssAI RCCGGY 3 cut(s) 446, 561, 762
BssECI CCNNGG 6 cut(s) 140, 390, 526, 691, 785, 786
BssMI GATC 4 cut(s) 498, 904, 1450, 1513
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 390, 526
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 692, 985, 1218
Bst4CI ACNGT 3 cut(s) 157, 181, 268
Bst6I CTCTTC 1 cut(s) 1042
BstAFI CTTAAG 1 cut(s) 833
BstC8I GCNNGC 8 cut(s) 448, 597, 709, 740, 764, 1300, 1354, 1577
BstDEI CTNAG 6 cut(s) 27, 724, 756, 824, 1122, 1470
BstDSI CCRYGG 1 cut(s) 390
BstEII GGTNACC 1 cut(s) 1163
BstF5I GGATG 3 cut(s) 610, 943, 1148
BstFNI CGCG 5 cut(s) 21, 284, 533, 707, 1356
BstH2I RGCGCY 1 cut(s) 763
BstHHI GCGC 2 cut(s) 533, 762
BstKTI GATC 4 cut(s) 501, 907, 1453, 1516
BstMAI GTCTC 5 cut(s) 469, 963, 1008, 1039, 1116
BstMBI GATC 4 cut(s) 498, 904, 1450, 1513
BstMCI CGRYCG 2 cut(s) 312, 566
BstMWI GCNNNNNNNGC 4 cut(s) 64, 308, 768, 851
BstNI CCWGG 3 cut(s) 692, 985, 1218
BstNSI RCATGY 2 cut(s) 823, 1579
BstPAI GACNNNNGTC 1 cut(s) 104
BstPI GGTNACC 1 cut(s) 1163
BstSCI CCNGG 7 cut(s) 140, 357, 690, 784, 785, 983, 1216
BstSFI CTRYAG 1 cut(s) 1502
BstUI CGCG 5 cut(s) 21, 284, 533, 707, 1356
BstV1I GCAGC 4 cut(s) 583, 863, 1488, 1510
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 4 cut(s) 164, 311, 450, 1329
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 610, 943, 1148
BtsIMutI CAGTG 2 cut(s) 1053, 1054
BtuMI TCGCGA 1 cut(s) 1356
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 8 cut(s) 448, 597, 709, 740, 764, 1300, 1354, 1577
CciI TCATGA 1 cut(s) 886
CfoI GCGC 2 cut(s) 533, 762
Cfr10I RCCGGY 3 cut(s) 446, 561, 762
Cfr13I GGNCC 8 cut(s) 162, 186, 203, 559, 728, 881, 1078, 1225
Cfr9I CCCGGG 1 cut(s) 785
CpoI CGGWCCG 1 cut(s) 186
CseI GACGC 1 cut(s) 522
Csp6I GTAC 5 cut(s) 167, 617, 988, 1099, 1547
CspAI ACCGGT 1 cut(s) 561
CspI CGGWCCG 1 cut(s) 186
CviAII CATG 9 cut(s) 293, 391, 461, 479, 820, 887, 951, 1310, 1576
CviQI GTAC 5 cut(s) 167, 617, 988, 1099, 1547
DdeI CTNAG 6 cut(s) 27, 724, 756, 824, 1122, 1470
DpnI GATC 4 cut(s) 500, 906, 1452, 1515
DpnII GATC 4 cut(s) 498, 904, 1450, 1513
DraI TTTAAA 1 cut(s) 670
EaeI YGGCCR 1 cut(s) 309
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 1042
EarI CTCTTC 1 cut(s) 1042
Ecl136II GAGCTC 3 cut(s) 355, 457, 1352
EclXI CGGCCG 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 390, 526
Eco24I GRGCYC 3 cut(s) 357, 459, 1354
Eco31I GGTCTC 2 cut(s) 1008, 1116
Eco47I GGWCC 7 cut(s) 186, 203, 559, 728, 881, 1078, 1225
Eco52I CGGCCG 1 cut(s) 309
Eco53kI GAGCTC 3 cut(s) 355, 457, 1352
Eco57I CTGAAG 4 cut(s) 131, 347, 867, 1077
Eco88I CYCGRG 3 cut(s) 452, 785, 1268
Eco91I GGTNACC 1 cut(s) 1163
EcoICRI GAGCTC 3 cut(s) 355, 457, 1352
EcoO109I RGGNCCY 2 cut(s) 203, 1078
EcoO65I GGTNACC 1 cut(s) 1163
EcoRI GAATTC 3 cut(s) 797, 1195, 1555
EcoRII CCWGG 3 cut(s) 690, 983, 1216
EcoT14I CCWWGG 2 cut(s) 390, 526
EcoT38I GRGCYC 3 cut(s) 357, 459, 1354
ErhI CCWWGG 2 cut(s) 390, 526
FaeI CATG 9 cut(s) 296, 394, 464, 482, 823, 890, 954, 1313, 1579
FaqI GGGAC 5 cut(s) 216, 520, 895, 1064, 1251
FatI CATG 9 cut(s) 292, 390, 460, 478, 819, 886, 950, 1309, 1575
FauI CCCGC 2 cut(s) 700, 1089
FauNDI CATATG 1 cut(s) 964
Fnu4HI GCNGC 7 cut(s) 22, 33, 309, 572, 852, 1499, 1502
FokI GGATG 3 cut(s) 617, 950, 1135
FriOI GRGCYC 3 cut(s) 357, 459, 1354
Fsp4HI GCNGC 7 cut(s) 22, 33, 309, 572, 852, 1499, 1502
FspBI CTAG 6 cut(s) 260, 297, 543, 767, 897, 1155
GlaI GCGC 2 cut(s) 532, 761
GluI GCNGC 7 cut(s) 22, 33, 309, 572, 852, 1499, 1502
GsaI CCCAGC 1 cut(s) 541
HaeII RGCGCY 1 cut(s) 763
HaeIII GGCC 4 cut(s) 164, 311, 450, 1329
HapII CCGG 9 cut(s) 142, 189, 312, 359, 447, 562, 763, 786, 855
HgaI GACGC 1 cut(s) 522
HhaI GCGC 2 cut(s) 533, 762
Hin1II CATG 9 cut(s) 296, 394, 464, 482, 823, 890, 954, 1313, 1579
Hin6I GCGC 2 cut(s) 531, 760
HinP1I GCGC 2 cut(s) 531, 760
HindIII AAGCTT 1 cut(s) 664
HinfI GANTC 7 cut(s) 631, 781, 956, 1253, 1264, 1541, 1562
HpaII CCGG 9 cut(s) 142, 189, 312, 359, 447, 562, 763, 786, 855
HphI GGTGA 3 cut(s) 298, 980, 1106
Hpy166II GTNNAC 5 cut(s) 153, 289, 343, 646, 1413
Hpy188III TCNNGA 7 cut(s) 201, 260, 887, 1155, 1268, 1347, 1355
Hpy8I GTNNAC 5 cut(s) 153, 289, 343, 646, 1413
HpyAV CCTTC 4 cut(s) 313, 1128, 1352, 1470
HpyCH4III ACNGT 3 cut(s) 157, 181, 268
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 8 cut(s) 279, 1090, 1190, 1203, 1302, 1400, 1504, 1579
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 308, 768, 851
HpyF3I CTNAG 6 cut(s) 27, 724, 756, 824, 1122, 1470
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 9 cut(s) 296, 394, 464, 482, 823, 890, 954, 1313, 1579
HspAI GCGC 2 cut(s) 531, 760
KroI GCCGGC 2 cut(s) 446, 762
KroNI GCCGGC 2 cut(s) 448, 764
Kzo9I GATC 4 cut(s) 498, 904, 1450, 1513
LmnI GCTCC 7 cut(s) 55, 64, 360, 462, 1349, 1430, 1482
Lsp1109I GCAGC 4 cut(s) 583, 863, 1488, 1510
LweI GCATC 1 cut(s) 720
MaeI CTAG 6 cut(s) 260, 297, 543, 767, 897, 1155
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 1049, 1163
MalI GATC 4 cut(s) 500, 906, 1452, 1515
MboI GATC 4 cut(s) 498, 904, 1450, 1513
MboII GAAGA 9 cut(s) 109, 666, 922, 967, 1029, 1390, 1432, 1538, 1541
MfeI CAATTG 1 cut(s) 1521
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 4 cut(s) 125, 357, 459, 1354
MlyI GAGTC 5 cut(s) 625, 965, 1247, 1273, 1550
MmeI TCCRAC 3 cut(s) 33, 659, 772
MnlI CCTC 7 cut(s) 153, 267, 461, 894, 1031, 1034, 1162
MroNI GCCGGC 2 cut(s) 446, 762
MroXI GAANNNNTTC 2 cut(s) 180, 1362
MseI TTAA 5 cut(s) 669, 834, 1290, 1488, 1588
MslI CAYNNNNRTG 2 cut(s) 716, 1058
MspA1I CMGCKG 1 cut(s) 1501
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 9 cut(s) 142, 189, 312, 359, 447, 562, 763, 786, 855
MspR9I CCNGG 7 cut(s) 142, 359, 692, 786, 787, 985, 1218
MunI CAATTG 1 cut(s) 1521
Mva1269I GAATGC 2 cut(s) 828, 1402
MvaI CCWGG 3 cut(s) 692, 985, 1218
MvnI CGCG 5 cut(s) 21, 284, 533, 707, 1356
MwoI GCNNNNNNNGC 4 cut(s) 64, 308, 768, 851
NaeI GCCGGC 2 cut(s) 448, 764
NciI CCSGG 4 cut(s) 142, 359, 786, 787
NcoI CCATGG 1 cut(s) 390
NdeI CATATG 1 cut(s) 964
NdeII GATC 4 cut(s) 498, 904, 1450, 1513
NgoMIV GCCGGC 2 cut(s) 446, 762
NlaIII CATG 9 cut(s) 296, 394, 464, 482, 823, 890, 954, 1313, 1579
NlaIV GGNNCC 4 cut(s) 204, 697, 1080, 1484
NmuCI GTSAC 1 cut(s) 1049
NruI TCGCGA 1 cut(s) 1356
NspI RCATGY 2 cut(s) 823, 1579
OliI CACNNNNGTG 1 cut(s) 1058
PaeI GCATGC 1 cut(s) 1579
PaeR7I CTCGAG 1 cut(s) 452
PagI TCATGA 1 cut(s) 886
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 828, 1402
PdiI GCCGGC 2 cut(s) 448, 764
PdmI GAANNNNTTC 2 cut(s) 180, 1362
PfeI GAWTC 2 cut(s) 781, 1562
PflFI GACNNNGTC 1 cut(s) 1031
PinAI ACCGGT 1 cut(s) 561
PkrI GCNGC 7 cut(s) 23, 34, 310, 573, 853, 1500, 1503
PleI GAGTC 5 cut(s) 625, 964, 1247, 1272, 1549
PpsI GAGTC 5 cut(s) 625, 964, 1247, 1272, 1549
PpuMI RGGWCCY 2 cut(s) 203, 1078
PshAI GACNNNNGTC 1 cut(s) 104
Psp124BI GAGCTC 3 cut(s) 357, 459, 1354
Psp5II RGGWCCY 2 cut(s) 203, 1078
Psp6I CCWGG 3 cut(s) 690, 983, 1216
PspEI GGTNACC 1 cut(s) 1163
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 3 cut(s) 690, 983, 1216
PspN4I GGNNCC 4 cut(s) 204, 697, 1080, 1484
PspPI GGNCC 8 cut(s) 162, 186, 203, 559, 728, 881, 1078, 1225
PspPPI RGGWCCY 2 cut(s) 203, 1078
PspXI VCTCGAGB 1 cut(s) 452
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 1 cut(s) 1506
PsuI RGATCY 1 cut(s) 498
PsyI GACNNNGTC 1 cut(s) 1031
PvuII CAGCTG 1 cut(s) 1501
RruI TCGCGA 1 cut(s) 1356
RsaI GTAC 5 cut(s) 168, 618, 989, 1100, 1548
RsaNI GTAC 5 cut(s) 167, 617, 988, 1099, 1547
RseI CAYNNNNRTG 2 cut(s) 716, 1058
Rsr2I CGGWCCG 1 cut(s) 186
RsrII CGGWCCG 1 cut(s) 186
SacI GAGCTC 3 cut(s) 357, 459, 1354
SaqAI TTAA 5 cut(s) 669, 834, 1290, 1488, 1588
SatI GCNGC 7 cut(s) 22, 33, 309, 572, 852, 1499, 1502
Sau3AI GATC 4 cut(s) 498, 904, 1450, 1513
Sau96I GGNCC 8 cut(s) 162, 186, 203, 559, 728, 881, 1078, 1225
SchI GAGTC 5 cut(s) 625, 965, 1247, 1273, 1550
ScrFI CCNGG 7 cut(s) 142, 359, 692, 786, 787, 985, 1218
SduI GDGCHC 4 cut(s) 125, 357, 459, 1354
SfaNI GCATC 1 cut(s) 720
SfcI CTRYAG 1 cut(s) 1502
Sfr274I CTCGAG 1 cut(s) 452
SinI GGWCC 7 cut(s) 186, 203, 559, 728, 881, 1078, 1225
SlaI CTCGAG 1 cut(s) 452
SmaI CCCGGG 1 cut(s) 787
SmiMI CAYNNNNRTG 2 cut(s) 716, 1058
SmlI CTYRAG 2 cut(s) 452, 833
SmoI CTYRAG 2 cut(s) 452, 833
SphI GCATGC 1 cut(s) 1579
SspMI CTAG 6 cut(s) 260, 297, 543, 767, 897, 1155
SstI GAGCTC 3 cut(s) 357, 459, 1354
StyD4I CCNGG 7 cut(s) 140, 357, 690, 784, 785, 983, 1216
StyI CCWWGG 2 cut(s) 390, 526
TaaI ACNGT 3 cut(s) 157, 181, 268
TaiI ACGT 1 cut(s) 108
TaqI TCGA 4 cut(s) 453, 681, 1026, 1262
TaqII GACCGA 2 cut(s) 62, 174
TatI WGTACW 1 cut(s) 616
TauI GCSGC 3 cut(s) 24, 35, 311
TfiI GAWTC 2 cut(s) 781, 1562
Tru1I TTAA 5 cut(s) 669, 834, 1290, 1488, 1588
Tru9I TTAA 5 cut(s) 669, 834, 1290, 1488, 1588
TscAI CASTG 2 cut(s) 1054, 1060
TseFI GTSAC 1 cut(s) 1049
TseI GCWGC 4 cut(s) 571, 851, 1498, 1501
Tsp45I GTSAC 1 cut(s) 1049
TspDTI ATGAA 3 cut(s) 621, 828, 939
TspGWI ACGGA 4 cut(s) 77, 102, 1026, 1534
TspMI CCCGGG 1 cut(s) 785
TspRI CASTG 2 cut(s) 1054, 1060
Tth111I GACNNNGTC 1 cut(s) 1031
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 7 cut(s) 186, 203, 559, 728, 881, 1078, 1225
XapI RAATTY 6 cut(s) 91, 797, 1065, 1195, 1381, 1555
XbaI TCTAGA 2 cut(s) 259, 1154
XceI RCATGY 2 cut(s) 823, 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 452
XmaI CCCGGG 1 cut(s) 785
XmnI GAANNNNTTC 2 cut(s) 180, 1362
XspI CTAG 6 cut(s) 260, 297, 543, 767, 897, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.