AT5G19730

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009451
Physical Location & Seq
Forward (+)
6670275 .. 6673625
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19730.1

Sequence Viewer

Length: 1152 bp
ATGCCCAAACTCAATTCAACCCAAGCTCCCAATTTATTCCTTCTCCTTCTCGTTATCCTTCTTTGTTCAACGCAAACACAATGCCACACCAAAGGTCTCCGATTACGACCAAGAAACCAGAAGAATATGAACACGACGAGTGATCGGACACAAAACCCGGAAGATGAGTTCATGAAATGGGTGAGATTCGTTGGAAGCCTTAAACACTCAGTGTTCAAGGCAGCCAAGAACAAACTCTTCCCTTCTTATACATTAACTGTCCACAAGAAATCCAATAAAGGTGACTTCACTAAAATCCAAGACGCCATTGATTCTCTCCCTCTCATCAACTTTGTTCGTGTCGTCATCAAAGTTCATGCCGGAGTTTACAAGGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAGACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTTACAACTCGGCCTCGTTCGCGGTCAACTCTCCTTTCTTTGTCGCTAAGAATATCACATTCAGGAACACGACCCCAGTCCCCTTGCCCGGTGCAGTTGGTAAACAGGCGGTAGCATTGAGGGTGTCGGCAGACAATGCCGCGTTTTTTGGGTGTAGAATGCTTGGTGCTCAAGACACTCTCTATGACCATTTGGGAAGACATTATTACAAAGATTGTTACATTGAGGGATCAGTGGACTTCATCTTTGGAAATGCCCTTTCTCTTTATGAAGGGTGCCACGTGCATGCGATAGCGGATAAGCTAGGAGCAGTGACGGCACAGGGGAGGAGCAGTGTTCTAGAGGACACCGGATTCTCGTTCGTAAAGTGTAAGGTGACAGGGACAGGGGTTTTGTATCTTGGGAGGGCATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACATGGACAACATCATCCTCCCTAGAGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGATGACGGTGTTCTATGGGCAGTACAAGTGCACGGGAGCAGGAGCAAACTATGGAGGGAGGGTGGCTTGGGCAAGAGAGCTCACCGACGAAGAAGCTAAGCCTTTTCTTTCTCTTACCTTTATCGACGGCTCTGAATGGATCAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

383

Amino Acids

42.54

Weight (kDa)

9.2

Isoelectric Point (pI)

26.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 90 - 377 1.7e-73 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 771
AccII CGCG 2 cut(s) 518, 638
AciI CCGC 4 cut(s) 518, 605, 636, 791
AclWI GGATC 2 cut(s) 733, 1148
AcuI CTGAAG 1 cut(s) 416
AcvI CACGTG 1 cut(s) 778
AcyI GRCGYC 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 2 cut(s) 977, 1025
AfiI CCNNNNNNNGG 2 cut(s) 397, 584
AgsI TTSAA 3 cut(s) 18, 69, 217
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 26, 799, 1081, 1097
AluI AGCT 4 cut(s) 26, 799, 1081, 1097
Alw21I GWGCWC 3 cut(s) 667, 1034, 1083
Alw26I GTCTC 3 cut(s) 101, 447, 981
Alw44I GTGCAC 1 cut(s) 1030
AlwI GGATC 2 cut(s) 733, 1148
AoxI GGCC 1 cut(s) 507
ApaLI GTGCAC 1 cut(s) 1030
ApeKI GCWGC 1 cut(s) 221
ArsI GACNNNNNNTTYG 4 cut(s) 725, 757, 871, 903
AspS9I GGNCC 1 cut(s) 911
AsuC2I CCSGG 2 cut(s) 158, 585
AsuHPI GGTGA 5 cut(s) 193, 293, 391, 883, 1075
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 2 cut(s) 497, 1034
BanI GGYRCC 1 cut(s) 771
BanII GRGCYC 1 cut(s) 1083
BbrPI CACGTG 1 cut(s) 778
BbsI GAAGAC 2 cut(s) 440, 700
Bbv12I GWGCWC 3 cut(s) 667, 1034, 1083
BbvI GCAGC 1 cut(s) 233
BceAI ACGGC 2 cut(s) 828, 1144
BcnI CCSGG 2 cut(s) 158, 585
BcoDI GTCTC 3 cut(s) 101, 447, 981
BfaI CTAG 3 cut(s) 800, 836, 966
BisI GCNGC 2 cut(s) 222, 636
BlpI GCTNAGC 1 cut(s) 1098
BlsI GCNGC 2 cut(s) 223, 637
Bme1390I CCNGG 2 cut(s) 158, 585
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 1 cut(s) 911
BmiI GGNNCC 3 cut(s) 773, 912, 913
BmrFI CCNGG 2 cut(s) 158, 585
BmrI ACTGGG 1 cut(s) 566
BmuI ACTGGG 1 cut(s) 566
BoxI GACNNNNGTC 1 cut(s) 572
BpiI GAAGAC 2 cut(s) 440, 700
Bpu1102I GCTNAGC 1 cut(s) 1098
BpuEI CTTGAG 2 cut(s) 651, 904
BpuMI CCSGG 2 cut(s) 158, 585
BsaAI YACGTR 1 cut(s) 778
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 2 cut(s) 101, 981
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 1 cut(s) 845
BsaXI ACNNNNNCTCC 6 cut(s) 10, 40, 814, 844, 1047, 1077
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 584
Bse1I ACTGG 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 957
BseLI CCNNNNNNNGG 2 cut(s) 397, 584
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 572
BseRI GAGGAG 1 cut(s) 838
BseSI GKGCMC 2 cut(s) 497, 1034
BseXI GCAGC 1 cut(s) 233
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 2 cut(s) 518, 638
BshFI GGCC 1 cut(s) 509
BshNI GGYRCC 1 cut(s) 771
BsiHKAI GWGCWC 3 cut(s) 667, 1034, 1083
BsiSI CCGG 4 cut(s) 158, 360, 585, 846
BslFI GGGAC 3 cut(s) 560, 892, 897
BslI CCNNNNNNNGG 2 cut(s) 397, 584
BsmAI GTCTC 3 cut(s) 101, 447, 981
BsmFI GGGAC 3 cut(s) 560, 892, 897
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 1 cut(s) 509
Bso31I GGTCTC 2 cut(s) 101, 981
Bsp1286I GDGCHC 4 cut(s) 497, 667, 1034, 1083
Bsp143I GATC 3 cut(s) 142, 725, 1140
Bsp1720I GCTNAGC 1 cut(s) 1098
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 4 cut(s) 518, 605, 636, 791
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 1 cut(s) 221
BspFNI CGCG 2 cut(s) 518, 638
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 773, 912, 913
BspPI GGATC 2 cut(s) 733, 1148
BspT107I GGYRCC 1 cut(s) 771
BspTNI GGTCTC 2 cut(s) 101, 981
BsrI ACTGG 1 cut(s) 572
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 3 cut(s) 142, 725, 1140
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 3 cut(s) 259, 442, 1009
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBAI YACGTR 1 cut(s) 778
BstC8I GCNNGC 1 cut(s) 783
BstDEI CTNAG 3 cut(s) 208, 543, 1098
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 957
BstFNI CGCG 2 cut(s) 518, 638
BstKTI GATC 3 cut(s) 145, 728, 1143
BstMAI GTCTC 3 cut(s) 101, 447, 981
BstMBI GATC 3 cut(s) 142, 725, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 515, 632, 812
BstNSI RCATGY 1 cut(s) 785
BstPAI GACNNNNGTC 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 156, 583
BstSLI GKGCMC 2 cut(s) 497, 1034
BstUI CGCG 2 cut(s) 518, 638
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 2 cut(s) 440, 700
BsuRI GGCC 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 1 cut(s) 957
BtsI GCAGTG 2 cut(s) 813, 835
BtsIMutI CAGTG 6 cut(s) 216, 392, 447, 735, 813, 835
Cac8I GCNNGC 1 cut(s) 783
CciI TCATGA 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 911
CseI GACGC 1 cut(s) 311
Csp6I GTAC 2 cut(s) 976, 1024
CviAII CATG 6 cut(s) 172, 356, 490, 782, 906, 946
CviQI GTAC 2 cut(s) 976, 1024
DdeI CTNAG 3 cut(s) 208, 543, 1098
DpnI GATC 3 cut(s) 144, 727, 1142
DpnII GATC 3 cut(s) 142, 725, 1140
DraIII CACNNNGTG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 1081
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 2 cut(s) 101, 981
Eco47I GGWCC 1 cut(s) 911
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 1 cut(s) 416
Eco72I CACGTG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 1081
EcoO109I RGGNCCY 1 cut(s) 911
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 175, 359, 493, 785, 909, 949
FaqI GGGAC 3 cut(s) 560, 892, 897
FatI CATG 6 cut(s) 171, 355, 489, 781, 905, 945
Fnu4HI GCNGC 2 cut(s) 222, 636
FokI GGATG 1 cut(s) 944
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 2 cut(s) 222, 636
FspBI CTAG 3 cut(s) 800, 836, 966
GluI GCNGC 2 cut(s) 222, 636
HaeIII GGCC 1 cut(s) 509
HapII CCGG 4 cut(s) 158, 360, 585, 846
HgaI GACGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 6 cut(s) 175, 359, 493, 785, 909, 949
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 5 cut(s) 186, 311, 473, 849, 924
HpaII CCGG 4 cut(s) 158, 360, 585, 846
HphI GGTGA 5 cut(s) 193, 293, 391, 883, 1075
Hpy166II GTNNAC 6 cut(s) 262, 367, 523, 599, 733, 1032
Hpy188I TCNGA 3 cut(s) 101, 147, 1135
Hpy188III TCNNGA 5 cut(s) 172, 559, 668, 836, 921
Hpy8I GTNNAC 6 cut(s) 262, 367, 523, 599, 733, 1032
Hpy99I CGWCG 3 cut(s) 139, 1091, 1130
HpyCH4III ACNGT 3 cut(s) 259, 442, 1009
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 3 cut(s) 590, 781, 1032
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 632, 812
HpyF3I CTNAG 3 cut(s) 208, 543, 1098
HpySE526I ACGT 1 cut(s) 777
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 6 cut(s) 175, 359, 493, 785, 909, 949
KflI GGGWCCC 1 cut(s) 911
Kzo9I GATC 3 cut(s) 142, 725, 1140
LmnI GCTCC 6 cut(s) 31, 425, 803, 825, 1037, 1043
Lsp1109I GCAGC 1 cut(s) 233
MaeI CTAG 3 cut(s) 800, 836, 966
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 4 cut(s) 281, 713, 808, 871
MalI GATC 3 cut(s) 144, 727, 1142
MboI GATC 3 cut(s) 142, 725, 1140
MboII GAAGA 6 cut(s) 133, 173, 229, 445, 705, 1103
MfeI CAATTG 1 cut(s) 979
MhlI GDGCHC 4 cut(s) 497, 667, 1034, 1083
MluCI AATT 3 cut(s) 13, 31, 979
MlyI GAGTC 2 cut(s) 467, 933
MmeI TCCRAC 1 cut(s) 172
MseI TTAA 2 cut(s) 201, 254
MslI CAYNNNNRTG 2 cut(s) 780, 944
MspI CCGG 4 cut(s) 158, 360, 585, 846
MspR9I CCNGG 2 cut(s) 158, 585
MunI CAATTG 1 cut(s) 979
Mva1269I GAATGC 1 cut(s) 660
MvnI CGCG 2 cut(s) 518, 638
MwoI GCNNNNNNNGC 3 cut(s) 515, 632, 812
NciI CCSGG 2 cut(s) 158, 585
NcoI CCATGG 1 cut(s) 489
NdeII GATC 3 cut(s) 142, 725, 1140
NlaIII CATG 6 cut(s) 175, 359, 493, 785, 909, 949
NlaIV GGNNCC 3 cut(s) 773, 912, 913
NmeAIII GCCGAG 1 cut(s) 485
NmuCI GTSAC 3 cut(s) 281, 808, 871
NspI RCATGY 1 cut(s) 785
PaeI GCATGC 1 cut(s) 785
PagI TCATGA 1 cut(s) 171
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 186, 311, 849
PkrI GCNGC 2 cut(s) 223, 637
PleI GAGTC 2 cut(s) 467, 932
PmaCI CACGTG 1 cut(s) 778
PmlI CACGTG 1 cut(s) 778
PpsI GAGTC 2 cut(s) 467, 932
Ppu21I YACGTR 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 911
PshAI GACNNNNGTC 1 cut(s) 572
PsiI TTATAA 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 1083
Psp5II RGGWCCY 1 cut(s) 911
PspCI CACGTG 1 cut(s) 778
PspN4I GGNNCC 3 cut(s) 773, 912, 913
PspPI GGNCC 1 cut(s) 911
PspPPI RGGWCCY 1 cut(s) 911
RsaI GTAC 2 cut(s) 977, 1025
RsaNI GTAC 2 cut(s) 976, 1024
RseI CAYNNNNRTG 2 cut(s) 780, 944
SacI GAGCTC 1 cut(s) 1083
SaqAI TTAA 2 cut(s) 201, 254
SatI GCNGC 2 cut(s) 222, 636
Sau3AI GATC 3 cut(s) 142, 725, 1140
Sau96I GGNCC 1 cut(s) 911
SchI GAGTC 2 cut(s) 467, 933
ScrFI CCNGG 2 cut(s) 158, 585
SduI GDGCHC 4 cut(s) 497, 667, 1034, 1083
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 2 cut(s) 780, 944
SmlI CTYRAG 2 cut(s) 666, 919
SmoI CTYRAG 2 cut(s) 666, 919
SphI GCATGC 1 cut(s) 785
Sse9I AATT 3 cut(s) 13, 31, 979
SsiI CCGC 4 cut(s) 518, 605, 636, 791
SspMI CTAG 3 cut(s) 800, 836, 966
SstI GAGCTC 1 cut(s) 1083
StyD4I CCNGG 2 cut(s) 156, 583
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 259, 442, 1009
TaiI ACGT 1 cut(s) 780
TaqI TCGA 1 cut(s) 1125
TasI AATT 3 cut(s) 13, 31, 979
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 1 cut(s) 638
TfiI GAWTC 3 cut(s) 186, 311, 849
Tru1I TTAA 2 cut(s) 201, 254
Tru9I TTAA 2 cut(s) 201, 254
TscAI CASTG 6 cut(s) 216, 399, 447, 735, 813, 835
TseFI GTSAC 3 cut(s) 281, 808, 871
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 3 cut(s) 281, 808, 871
TspDTI ATGAA 6 cut(s) 143, 160, 188, 344, 727, 780
TspRI CASTG 6 cut(s) 216, 399, 447, 735, 813, 835
VneI GTGCAC 1 cut(s) 1030
VpaK11BI GGWCC 1 cut(s) 911
XbaI TCTAGA 1 cut(s) 835
XceI RCATGY 1 cut(s) 785
XspI CTAG 3 cut(s) 800, 836, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.