Rh2DG067100

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009451
Physical Location & Seq
Reverse (-)
5043706 .. 5046162
2457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG067100.1

Sequence Viewer

Length: 1017 bp
ATGACCCGAGCCCAATTCTCGGAGCAACAATTCATGAAGTGGGTCAAGTTTGTTGGGAGACTCAAACACTCGGTTTATAAGAACGCGAAGAACAAGCTCTTCCCCTCTTACACACTCAATGTGGCTAAGAACCCTTCAGGGGGAGACTTTACCAAAATTCAGGACGCCATTGATTCTCTTCCGTTCATCAATCTCGTCAGAGTCGTCATCAAGGTCCATGCAGGTGTCTACAAGGAAAAGGTGAATATACCGCCATTGAAATCGTTTATAACCATAGAAGGAGCAGGGGCGGACAAGACAATAGTTCAATGGGGAGACACAGCTCAAACACCTTCAGGTCCAAAAAAACAACCGATGGGGACTTATAATTCTGCAACTTTTGCTGTGAATTCACCTTATTTCATTGCCAGGAACATTACTTTCAAGAACACCACGCCGGTACCGGCTCCAGGAGCAATCGGAAAGCAGGCAGTGGCGTTTAGAATATCGGGAGATACAGCAGCCTTCTTAGGGTGTAGATTTTTGGGAGCACAGGACACATTGTACGACCATTTGGGAAGGCATTATTACAAAGATTGTTACATTGAAGGTTCTGTTGATTTCATCTTCGGAAATGGTCTCTCTCTGTTTGAGGGATGTCACGTGCATGCAATAGCAACAAGCATAGGGGCATTAACGGCGCAAGGGAGGAGCAGTTTACTGGAGGAGACGGGGTTCTCGTTTGTCAACTGTAAGGTCACGGGTTCAGGAGCCCTCTATCTTGGAAGGGCTTGGGGACCTTTTTCTAGGGTAGTCTTTGCCTACACTTATATGGACAACATTATCACGCCCAAAGGCTGGTACAACTGGGGTGACCCTAATCGTGAAATGACTGTGTTTTATGGGCAATACAAGTGCACCGGACCTGGGGCTCAGTATGCAGGGAGGGTATCGTGGTCTAGGGAACTCACTGATGAGGAAGCAAAGCCTTTTATTTCCCTTACCTTCATAGATGGGTCTGAGTGGCTCAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

338

Amino Acids

37.42

Weight (kDa)

9.24

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 41 - 332 3e-74 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 78, 269, 366
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 439
AccB7I CCANNNNNTGG 1 cut(s) 837
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 86
AciI CCGC 2 cut(s) 251, 290
AcsI RAATTY 3 cut(s) 156, 388, 1010
AcuI CTGAAG 2 cut(s) 120, 318
AcvI CACGTG 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 3 cut(s) 441, 545, 842
AfiI CCNNNNNNNGG 7 cut(s) 19, 139, 140, 449, 510, 837, 906
AgsI TTSAA 4 cut(s) 259, 308, 424, 587
AjnI CCWGG 3 cut(s) 407, 448, 904
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 2 cut(s) 97, 323
AluI AGCT 2 cut(s) 97, 323
Alw21I GWGCWC 2 cut(s) 532, 899
Alw26I GTCTC 5 cut(s) 52, 138, 309, 623, 701
Alw44I GTGCAC 1 cut(s) 895
Ama87I CYCGRG 1 cut(s) 6
ApaLI GTGCAC 1 cut(s) 895
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 3 cut(s) 156, 388, 1010
Asp718I GGTACC 1 cut(s) 439
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 4 cut(s) 214, 338, 776, 902
AsuHPI GGTGA 3 cut(s) 253, 384, 863
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 4 cut(s) 214, 338, 776, 902
BaeGI GKGCMC 1 cut(s) 899
BaeI ACNNNNGTAYC 2 cut(s) 423, 456
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 3 cut(s) 13, 754, 913
BbrPI CACGTG 1 cut(s) 643
Bbv12I GWGCWC 2 cut(s) 532, 899
BbvI GCAGC 1 cut(s) 512
BccI CCATC 2 cut(s) 349, 986
BceAI ACGGC 1 cut(s) 693
BciT130I CCWGG 3 cut(s) 409, 450, 906
BcoDI GTCTC 5 cut(s) 52, 138, 309, 623, 701
BfaI CTAG 2 cut(s) 786, 939
BfuAI ACCTGC 1 cut(s) 212
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 3 cut(s) 409, 450, 906
Bme18I GGWCC 4 cut(s) 214, 338, 776, 902
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 4 cut(s) 214, 338, 776, 902
BmiI GGNNCC 4 cut(s) 441, 447, 751, 777
BmrFI CCNGG 3 cut(s) 409, 450, 906
BmrI ACTGGG 1 cut(s) 856
BmuI ACTGGG 1 cut(s) 856
BpmI CTGGAG 2 cut(s) 432, 722
BsaAI YACGTR 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 905
BsaWI WCCGGW 1 cut(s) 899
BsaXI ACNNNNNCTCC 4 cut(s) 679, 698, 709, 728
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 139, 140, 449, 510, 837, 906
Bse118I RCCGGY 2 cut(s) 436, 442
Bse1I ACTGG 2 cut(s) 705, 851
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 3 cut(s) 409, 450, 906
BseDI CCNNGG 1 cut(s) 905
BseGI GGATG 1 cut(s) 641
BseLI CCNNNNNNNGG 7 cut(s) 19, 139, 140, 449, 510, 837, 906
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 2 cut(s) 926, 990
BseNI ACTGG 2 cut(s) 705, 851
BseRI GAGGAG 2 cut(s) 703, 719
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 439
BsiHKAI GWGCWC 2 cut(s) 532, 899
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 3 cut(s) 437, 443, 900
BslFI GGGAC 2 cut(s) 373, 789
BslI CCNNNNNNNGG 7 cut(s) 19, 139, 140, 449, 510, 837, 906
BsmAI GTCTC 5 cut(s) 52, 138, 309, 623, 701
BsmBI CGTCTC 1 cut(s) 701
BsmFI GGGAC 2 cut(s) 373, 789
Bso31I GGTCTC 1 cut(s) 623
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 5 cut(s) 13, 532, 754, 899, 913
BspACI CCGC 2 cut(s) 251, 290
BspCNI CTCAG 2 cut(s) 925, 991
BspFNI CGCG 1 cut(s) 86
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 4 cut(s) 441, 447, 751, 777
BspMI ACCTGC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 402
BsrFI RCCGGY 2 cut(s) 436, 442
BsrI ACTGG 2 cut(s) 705, 851
BssAI RCCGGY 2 cut(s) 436, 442
BssECI CCNNGG 1 cut(s) 905
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 409, 450, 906
Bst4CI ACNGT 2 cut(s) 731, 874
Bst6I CTCTTC 2 cut(s) 104, 183
BstACI GRCGYC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 643
BstC8I GCNNGC 2 cut(s) 468, 648
BstDEI CTNAG 4 cut(s) 126, 508, 912, 999
BstEII GGTNACC 1 cut(s) 851
BstF5I GGATG 1 cut(s) 641
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 682
BstMAI GTCTC 5 cut(s) 52, 138, 309, 623, 701
BstMWI GCNNNNNNNGC 4 cut(s) 380, 452, 677, 917
BstNI CCWGG 3 cut(s) 409, 450, 906
BstNSI RCATGY 1 cut(s) 650
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 3 cut(s) 407, 448, 904
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 512
BtsCI GGATG 1 cut(s) 641
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 2 cut(s) 477, 948
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 2 cut(s) 468, 648
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 2 cut(s) 436, 442
Cfr13I GGNCC 4 cut(s) 214, 338, 776, 902
CseI GACGC 1 cut(s) 173
Csp6I GTAC 3 cut(s) 440, 544, 841
CviAII CATG 3 cut(s) 34, 218, 647
CviQI GTAC 3 cut(s) 440, 544, 841
DdeI CTNAG 4 cut(s) 126, 508, 912, 999
Eam1104I CTCTTC 2 cut(s) 104, 183
EarI CTCTTC 2 cut(s) 104, 183
EciI GGCGGA 1 cut(s) 305
Eco24I GRGCYC 3 cut(s) 13, 754, 913
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 4 cut(s) 214, 338, 776, 902
Eco57I CTGAAG 2 cut(s) 120, 318
Eco72I CACGTG 1 cut(s) 643
Eco88I CYCGRG 1 cut(s) 6
Eco91I GGTNACC 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 776
EcoO65I GGTNACC 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 407, 448, 904
EcoT38I GRGCYC 3 cut(s) 13, 754, 913
Esp3I CGTCTC 1 cut(s) 701
FaeI CATG 3 cut(s) 37, 221, 650
FaqI GGGAC 2 cut(s) 373, 789
FatI CATG 3 cut(s) 33, 217, 646
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 1 cut(s) 648
FriOI GRGCYC 3 cut(s) 13, 754, 913
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 2 cut(s) 786, 939
GlaI GCGC 1 cut(s) 681
GluI GCNGC 1 cut(s) 501
GsuI CTGGAG 2 cut(s) 432, 722
HapII CCGG 3 cut(s) 437, 443, 900
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 3 cut(s) 37, 221, 650
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HinfI GANTC 3 cut(s) 60, 173, 201
HpaII CCGG 3 cut(s) 437, 443, 900
HphI GGTGA 3 cut(s) 253, 384, 863
Hpy166II GTNNAC 4 cut(s) 229, 698, 727, 897
Hpy188I TCNGA 6 cut(s) 22, 200, 461, 611, 1000, 1016
Hpy188III TCNNGA 6 cut(s) 34, 161, 424, 489, 747, 863
Hpy8I GTNNAC 4 cut(s) 229, 698, 727, 897
HpyAV CCTTC 8 cut(s) 144, 272, 342, 514, 552, 581, 759, 994
HpyCH4III ACNGT 2 cut(s) 731, 874
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 6 cut(s) 221, 374, 646, 650, 897, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 452, 677, 917
HpyF3I CTNAG 4 cut(s) 126, 508, 912, 999
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 3 cut(s) 37, 221, 650
HspAI GCGC 1 cut(s) 680
KpnI GGTACC 1 cut(s) 443
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 7 cut(s) 22, 281, 451, 452, 527, 690, 749
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 2 cut(s) 786, 939
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 4 cut(s) 578, 638, 736, 851
MboII GAAGA 4 cut(s) 91, 100, 170, 598
MhlI GDGCHC 5 cut(s) 13, 532, 754, 899, 913
MluCI AATT 6 cut(s) 14, 29, 156, 367, 388, 1010
MlyI GAGTC 2 cut(s) 54, 210
MnlI CCTC 7 cut(s) 115, 625, 681, 697, 764, 918, 949
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 3 cut(s) 222, 645, 809
MspI CCGG 3 cut(s) 437, 443, 900
MspR9I CCNGG 3 cut(s) 409, 450, 906
MvaI CCWGG 3 cut(s) 409, 450, 906
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 4 cut(s) 380, 452, 677, 917
NlaIII CATG 3 cut(s) 37, 221, 650
NlaIV GGNNCC 4 cut(s) 441, 447, 751, 777
NmuCI GTSAC 3 cut(s) 638, 736, 851
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 716
PfeI GAWTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 837
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 2 cut(s) 54, 209
PmaCI CACGTG 1 cut(s) 643
PmlI CACGTG 1 cut(s) 643
PpsI GAGTC 2 cut(s) 54, 209
Ppu21I YACGTR 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 776
PsiI TTATAA 3 cut(s) 78, 269, 366
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 3 cut(s) 407, 448, 904
PspCI CACGTG 1 cut(s) 643
PspEI GGTNACC 1 cut(s) 851
PspGI CCWGG 3 cut(s) 407, 448, 904
PspN4I GGNNCC 4 cut(s) 441, 447, 751, 777
PspPI GGNCC 4 cut(s) 214, 338, 776, 902
PspPPI RGGWCCY 1 cut(s) 776
RsaI GTAC 3 cut(s) 441, 545, 842
RsaNI GTAC 3 cut(s) 440, 544, 841
RseI CAYNNNNRTG 3 cut(s) 222, 645, 809
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 1 cut(s) 501
Sau96I GGNCC 4 cut(s) 214, 338, 776, 902
SchI GAGTC 2 cut(s) 54, 210
ScrFI CCNGG 3 cut(s) 409, 450, 906
SduI GDGCHC 5 cut(s) 13, 532, 754, 899, 913
SinI GGWCC 4 cut(s) 214, 338, 776, 902
SmiMI CAYNNNNRTG 3 cut(s) 222, 645, 809
SphI GCATGC 1 cut(s) 650
Sse9I AATT 6 cut(s) 14, 29, 156, 367, 388, 1010
SsiI CCGC 2 cut(s) 251, 290
SspMI CTAG 2 cut(s) 786, 939
StyD4I CCNGG 3 cut(s) 407, 448, 904
TaaI ACNGT 2 cut(s) 731, 874
TaiI ACGT 1 cut(s) 645
TasI AATT 6 cut(s) 14, 29, 156, 367, 388, 1010
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 2 cut(s) 477, 955
TseFI GTSAC 3 cut(s) 638, 736, 851
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 3 cut(s) 638, 736, 851
TspDTI ATGAA 6 cut(s) 22, 50, 175, 391, 592, 976
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 2 cut(s) 477, 955
Van91I CCANNNNNTGG 1 cut(s) 837
VneI GTGCAC 1 cut(s) 895
VpaK11BI GGWCC 4 cut(s) 214, 338, 776, 902
XapI RAATTY 3 cut(s) 156, 388, 1010
XceI RCATGY 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 786, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.