Rroxscaffold_2G00149530

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009451
Physical Location & Seq
Forward (+)
87080382 .. 87083324
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149530.1

Sequence Viewer

Length: 1200 bp
ATGCCAACATTATTCCAAAGCATTTTCTATGCTACTCTTCTTCTTCTTGTCCTAAACTCAACACAGACCTTATGTCACACCAAGAGAATTCGGCCGGGAAACTCCACCGGAAAAGTACACCTGGCCAAGAACATGACCCGAGCCCAATTCTCGGAGCAACAATTCATGAAGTGGGTCAAGTTTGTTGGGAGACTCAAACACTCGGTTTATAAGAACGCGAAGAACAAGCTCTTCCCCTCTTACACGCTCAATGTGGCTAAGAACCCTTCACGGGGAGACTTCACGAAAATTCAGGACGCCATTGATTCTCTTCCGTTCATCAATCTCGTCAGAGTCGTCATCAAGGTCCAGGCAGGTGTCTACAAGGAAAAGGTGAATATACCGCCATTGAAATCGTTTATAACCATAGAAGGAGCAGGGGCGGACAAGACAATAGTTCAATGGGGAGACACAGCTCAAACACCTTCAGGTCCGAAAAAACAACCGATGGGGACCTATAACTCTGCAACTTTTGCTGTGAATTCACCTTATTTCATTGCCAGGAACATTACTTTCAAGAACACCACGCCGGTACCGGCTCCAGGAGCAATCGGAAAGCAGGCAGTGGCGTTTAGAATATCGGGAGATACAGCAGCCTTCTTAGGGTGTAGATTTTTAGGAGCACAGGACACATTGTACGACCATTTGGGAAGGCATTATTACAAAGATTGTTACATTGAAGGTTCTGTTGATTTCATCTTCGGAAATGGTCTCTCTCTGTTTGAGTCTCGATCACAAGCTCTATCACTCACATGTAAGTGTGCGAATGTGGTGCAGGGATGTCACGTGCATGCAATAGCAACAAGCATAGGGGCATTAACGGCGCAAGGGAGGAGCAGTTTACTGGAGGAGACGGGGTTCTCGTTTGTCAACTGTAAGGTCACGGGTTCAGGAGCTCTCTATCTTGGAAGGGCTTGGGGACCTTTTTCTAGGGTAGTCTTTGCCTACACTTATATGGACAACATTATCATGCCCAAAGGCTGGTACAACTGGGGTGACCCTAATCGTGAAATGACTGTGTTTTATGGGCAATACAAGTGCACAGGACCTGGGGCTCAGTATGCAGGGAGGGTATCGTGGTCTAGGGAACTCACTGATGAGGAAGCAAAGCCTTTTATTTCCCTTTCCTTCATAGATGGGTCTGAGTGGCTCAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

399

Amino Acids

44.23

Weight (kDa)

9.51

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 85 - 393 5.3e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 210, 401
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 1020
AccI GTMKAC 1 cut(s) 360
AccII CGCG 1 cut(s) 218
AciI CCGC 2 cut(s) 383, 422
AcoI YGGCCR 2 cut(s) 92, 123
AcsI RAATTY 4 cut(s) 87, 288, 520, 1193
AcuI CTGAAG 1 cut(s) 450
AcvI CACGTG 1 cut(s) 826
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 4 cut(s) 117, 573, 677, 1025
AfiI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 1020
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 4 cut(s) 391, 440, 556, 719
AhdI GACNNNNNGTC 1 cut(s) 72
AjnI CCWGG 5 cut(s) 120, 348, 539, 580, 1087
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
AluBI AGCT 4 cut(s) 229, 455, 779, 935
AluI AGCT 4 cut(s) 229, 455, 779, 935
Alw21I GWGCWC 3 cut(s) 664, 937, 1082
Alw26I GTCTC 6 cut(s) 184, 270, 441, 755, 771, 884
Alw44I GTGCAC 1 cut(s) 1078
AlwNI CAGNNNCTG 1 cut(s) 1088
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 92, 123
ApaLI GTGCAC 1 cut(s) 1078
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 4 cut(s) 87, 288, 520, 1193
Asp718I GGTACC 1 cut(s) 571
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 5 cut(s) 346, 470, 492, 959, 1085
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 385, 516, 1046
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 5 cut(s) 346, 470, 492, 959, 1085
BaeGI GKGCMC 1 cut(s) 1082
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 3 cut(s) 145, 937, 1096
BbrPI CACGTG 1 cut(s) 826
Bbv12I GWGCWC 3 cut(s) 664, 937, 1082
BbvI GCAGC 1 cut(s) 644
BccI CCATC 2 cut(s) 481, 1169
BceAI ACGGC 1 cut(s) 876
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 5 cut(s) 122, 350, 541, 582, 1089
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 6 cut(s) 184, 270, 441, 755, 771, 884
BfaI CTAG 2 cut(s) 969, 1122
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 1 cut(s) 633
BlsI GCNGC 1 cut(s) 634
Bme1390I CCNGG 6 cut(s) 96, 122, 350, 541, 582, 1089
Bme18I GGWCC 5 cut(s) 346, 470, 492, 959, 1085
BmeRI GACNNNNNGTC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 5 cut(s) 346, 470, 492, 959, 1085
BmiI GGNNCC 4 cut(s) 493, 573, 579, 960
BmrFI CCNGG 6 cut(s) 96, 122, 350, 541, 582, 1089
BmrI ACTGGG 1 cut(s) 1039
BmuI ACTGGG 1 cut(s) 1039
BpmI CTGGAG 2 cut(s) 564, 905
BpuMI CCSGG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 826
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 1088
BsaWI WCCGGW 1 cut(s) 107
BsaXI ACNNNNNCTCC 4 cut(s) 862, 881, 892, 911
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 1020
Bse118I RCCGGY 2 cut(s) 568, 574
Bse1I ACTGG 2 cut(s) 888, 1034
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 5 cut(s) 122, 350, 541, 582, 1089
BseDI CCNNGG 1 cut(s) 1088
BseGI GGATG 1 cut(s) 824
BseLI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 1020
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 1109, 1173
BseNI ACTGG 2 cut(s) 888, 1034
BseRI GAGGAG 2 cut(s) 886, 902
BseSI GKGCMC 1 cut(s) 1082
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 833
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 2 cut(s) 94, 125
BshNI GGYRCC 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 3 cut(s) 664, 937, 1082
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 4 cut(s) 95, 108, 569, 575
BslFI GGGAC 2 cut(s) 505, 972
BslI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 1020
BsmAI GTCTC 6 cut(s) 184, 270, 441, 755, 771, 884
BsmBI CGTCTC 1 cut(s) 884
BsmFI GGGAC 2 cut(s) 505, 972
BsnI GGCC 2 cut(s) 94, 125
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 5 cut(s) 145, 664, 937, 1082, 1096
Bsp143I GATC 1 cut(s) 770
BspACI CCGC 2 cut(s) 383, 422
BspANI GGCC 2 cut(s) 94, 125
BspCNI CTCAG 2 cut(s) 1108, 1174
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 493, 573, 579, 960
BspMI ACCTGC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 2 cut(s) 568, 574
BsrI ACTGG 2 cut(s) 888, 1034
BssAI RCCGGY 2 cut(s) 568, 574
BssECI CCNNGG 1 cut(s) 1088
BssMI GATC 1 cut(s) 770
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 5 cut(s) 122, 350, 541, 582, 1089
Bst4CI ACNGT 2 cut(s) 914, 1057
Bst6I CTCTTC 3 cut(s) 42, 236, 315
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 2 cut(s) 600, 831
BstDEI CTNAG 4 cut(s) 258, 640, 1095, 1182
BstEII GGTNACC 1 cut(s) 1034
BstF5I GGATG 1 cut(s) 824
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 865
BstKTI GATC 1 cut(s) 773
BstMAI GTCTC 6 cut(s) 184, 270, 441, 755, 771, 884
BstMBI GATC 1 cut(s) 770
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 512, 584, 860, 1100
BstNI CCWGG 5 cut(s) 122, 350, 541, 582, 1089
BstNSI RCATGY 2 cut(s) 795, 833
BstPI GGTNACC 1 cut(s) 1034
BstSCI CCNGG 6 cut(s) 94, 120, 348, 539, 580, 1087
BstSLI GKGCMC 1 cut(s) 1082
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 644
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 94, 125
BtsCI GGATG 1 cut(s) 824
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 609, 1131
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 600, 831
CaiI CAGNNNCTG 1 cut(s) 1088
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 865
Cfr10I RCCGGY 2 cut(s) 568, 574
Cfr13I GGNCC 5 cut(s) 346, 470, 492, 959, 1085
CseI GACGC 1 cut(s) 305
Csp6I GTAC 4 cut(s) 116, 572, 676, 1024
CviAII CATG 5 cut(s) 133, 166, 792, 830, 1009
CviQI GTAC 4 cut(s) 116, 572, 676, 1024
DdeI CTNAG 4 cut(s) 258, 640, 1095, 1182
DpnI GATC 1 cut(s) 772
DpnII GATC 1 cut(s) 770
DriI GACNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 92, 123
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 3 cut(s) 42, 236, 315
Eam1105I GACNNNNNGTC 1 cut(s) 72
EarI CTCTTC 3 cut(s) 42, 236, 315
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 935
EclXI CGGCCG 1 cut(s) 92
Eco24I GRGCYC 3 cut(s) 145, 937, 1096
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 5 cut(s) 346, 470, 492, 959, 1085
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 1 cut(s) 450
Eco72I CACGTG 1 cut(s) 826
Eco88I CYCGRG 1 cut(s) 138
Eco91I GGTNACC 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 935
EcoO109I RGGNCCY 3 cut(s) 492, 959, 1085
EcoO65I GGTNACC 1 cut(s) 1034
EcoRI GAATTC 2 cut(s) 87, 520
EcoRII CCWGG 5 cut(s) 120, 348, 539, 580, 1087
EcoT38I GRGCYC 3 cut(s) 145, 937, 1096
Esp3I CGTCTC 1 cut(s) 884
FaeI CATG 5 cut(s) 136, 169, 795, 833, 1012
FaqI GGGAC 2 cut(s) 505, 972
FatI CATG 5 cut(s) 132, 165, 791, 829, 1008
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 633
FokI GGATG 1 cut(s) 831
FriOI GRGCYC 3 cut(s) 145, 937, 1096
Fsp4HI GCNGC 1 cut(s) 633
FspBI CTAG 2 cut(s) 969, 1122
GlaI GCGC 1 cut(s) 864
GluI GCNGC 1 cut(s) 633
GsuI CTGGAG 2 cut(s) 564, 905
HaeIII GGCC 2 cut(s) 94, 125
HapII CCGG 4 cut(s) 95, 108, 569, 575
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 865
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 136, 169, 795, 833, 1012
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 4 cut(s) 192, 305, 333, 764
HpaII CCGG 4 cut(s) 95, 108, 569, 575
HphI GGTGA 3 cut(s) 385, 516, 1046
Hpy166II GTNNAC 5 cut(s) 118, 361, 881, 910, 1080
Hpy188I TCNGA 7 cut(s) 154, 332, 474, 593, 743, 1183, 1199
Hpy188III TCNNGA 8 cut(s) 166, 283, 293, 556, 621, 768, 930, 1046
Hpy8I GTNNAC 5 cut(s) 118, 361, 881, 910, 1080
HpyAV CCTTC 8 cut(s) 276, 404, 474, 646, 684, 713, 942, 1177
HpyCH4III ACNGT 2 cut(s) 914, 1057
HpyCH4IV ACGT 1 cut(s) 825
HpyCH4V TGCA 6 cut(s) 506, 814, 829, 833, 1080, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 584, 860, 1100
HpyF3I CTNAG 4 cut(s) 258, 640, 1095, 1182
HpySE526I ACGT 1 cut(s) 825
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 136, 169, 795, 833, 1012
HspAI GCGC 1 cut(s) 863
KpnI GGTACC 1 cut(s) 575
Kzo9I GATC 1 cut(s) 770
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 7 cut(s) 154, 413, 583, 584, 659, 873, 932
Lsp1109I GCAGC 1 cut(s) 644
MaeI CTAG 2 cut(s) 969, 1122
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 5 cut(s) 74, 710, 821, 919, 1034
MalI GATC 1 cut(s) 772
MboI GATC 1 cut(s) 770
MboII GAAGA 7 cut(s) 29, 32, 35, 223, 232, 302, 730
MhlI GDGCHC 5 cut(s) 145, 664, 937, 1082, 1096
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 6 cut(s) 87, 146, 161, 288, 520, 1193
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 3 cut(s) 186, 342, 773
MnlI CCTC 5 cut(s) 247, 864, 880, 1101, 1132
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 1 cut(s) 857
MslI CAYNNNNRTG 5 cut(s) 790, 796, 828, 992, 1007
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 4 cut(s) 95, 108, 569, 575
MspR9I CCNGG 6 cut(s) 96, 122, 350, 541, 582, 1089
MvaI CCWGG 5 cut(s) 122, 350, 541, 582, 1089
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 4 cut(s) 512, 584, 860, 1100
NciI CCSGG 1 cut(s) 96
NdeII GATC 1 cut(s) 770
NlaIII CATG 5 cut(s) 136, 169, 795, 833, 1012
NlaIV GGNNCC 4 cut(s) 493, 573, 579, 960
NmuCI GTSAC 4 cut(s) 74, 821, 919, 1034
NspI RCATGY 2 cut(s) 795, 833
PaeI GCATGC 1 cut(s) 833
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 791
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 899
PfeI GAWTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 1020
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 1 cut(s) 634
PleI GAGTC 3 cut(s) 186, 341, 772
PmaCI CACGTG 1 cut(s) 826
PmlI CACGTG 1 cut(s) 826
PpsI GAGTC 3 cut(s) 186, 341, 772
Ppu21I YACGTR 1 cut(s) 826
PpuMI RGGWCCY 3 cut(s) 492, 959, 1085
PscI ACATGT 1 cut(s) 791
PsiI TTATAA 2 cut(s) 210, 401
Psp124BI GAGCTC 1 cut(s) 937
Psp5II RGGWCCY 3 cut(s) 492, 959, 1085
Psp6I CCWGG 5 cut(s) 120, 348, 539, 580, 1087
PspCI CACGTG 1 cut(s) 826
PspEI GGTNACC 1 cut(s) 1034
PspGI CCWGG 5 cut(s) 120, 348, 539, 580, 1087
PspN4I GGNNCC 4 cut(s) 493, 573, 579, 960
PspPI GGNCC 5 cut(s) 346, 470, 492, 959, 1085
PspPPI RGGWCCY 3 cut(s) 492, 959, 1085
PstNI CAGNNNCTG 1 cut(s) 1088
RsaI GTAC 4 cut(s) 117, 573, 677, 1025
RsaNI GTAC 4 cut(s) 116, 572, 676, 1024
RseI CAYNNNNRTG 5 cut(s) 790, 796, 828, 992, 1007
SacI GAGCTC 1 cut(s) 937
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 857
SatI GCNGC 1 cut(s) 633
Sau3AI GATC 1 cut(s) 770
Sau96I GGNCC 5 cut(s) 346, 470, 492, 959, 1085
SchI GAGTC 3 cut(s) 186, 342, 773
ScrFI CCNGG 6 cut(s) 96, 122, 350, 541, 582, 1089
SduI GDGCHC 5 cut(s) 145, 664, 937, 1082, 1096
SinI GGWCC 5 cut(s) 346, 470, 492, 959, 1085
SmiMI CAYNNNNRTG 5 cut(s) 790, 796, 828, 992, 1007
SphI GCATGC 1 cut(s) 833
Sse9I AATT 6 cut(s) 87, 146, 161, 288, 520, 1193
SsiI CCGC 2 cut(s) 383, 422
SspMI CTAG 2 cut(s) 969, 1122
SstI GAGCTC 1 cut(s) 937
StyD4I CCNGG 6 cut(s) 94, 120, 348, 539, 580, 1087
TaaI ACNGT 2 cut(s) 914, 1057
TaiI ACGT 1 cut(s) 828
TaqI TCGA 1 cut(s) 769
TasI AATT 6 cut(s) 87, 146, 161, 288, 520, 1193
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 857
Tru9I TTAA 1 cut(s) 857
TscAI CASTG 2 cut(s) 609, 1138
TseFI GTSAC 4 cut(s) 74, 821, 919, 1034
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 4 cut(s) 74, 821, 919, 1034
TspDTI ATGAA 6 cut(s) 154, 182, 307, 523, 724, 1159
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 2 cut(s) 609, 1138
Van91I CCANNNNNTGG 1 cut(s) 1020
VneI GTGCAC 1 cut(s) 1078
VpaK11BI GGWCC 5 cut(s) 346, 470, 492, 959, 1085
XapI RAATTY 4 cut(s) 87, 288, 520, 1193
XceI RCATGY 2 cut(s) 795, 833
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 2 cut(s) 969, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.