Rh2AG068100

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009451
Physical Location & Seq
Reverse (-)
5664185 .. 5667225
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG068100.1

Sequence Viewer

Length: 1158 bp
ATGCCAACATTATTCCAAAGCATTTTCTATGCTACTCTTCTTCTTCTTGTCCTAAACTCAACACAGACCTTATGTCACACCAAGGGAATTCGGCCGGGAAACTCCGCCGGAAAAGTACACCTGGCCAAGAACATGACCCGAGCCCAATTCTCGGAGCAACAATTCATGAAGTGGGTCAAGTTTGTTGGGAGACTCAAACACTCGGTTTATAAGAACGCGAAGAACAAGCTCTTCCCCTCTTACACACTCAATGTGGCTAAGAACCCTTCACGGGGAGACTTTACGAAAATTCAGGACGCCATTGATTCTCTTCCGTTCATCAATCTCGTCAGAGTCGTCATCAAGGTCCATGCAGGTGTCTACAAGGAAAAGGTGAATATACCGCCATTGAAATCGTTTATAACCATAGAAGGAGCAGGGGCGGACAAGACAATAGTTCAATGGGGAGACACAGCTCAAACACCTTCAGGTCCAAAAAAACAACCGATGGGGACTTATAATTCTGCAACTTTTGCTGTGAATTCACCTTATTTCATTGCCAGGAACATTACTTTCAAGAACACCACGCCGGTACCGGCTCCAGGAGCAATCGGAAAGCAGGCAGTGGCGTTTAGAATATCGGGAGATACAGCAGCCTTCTTAGGGTGTAGATTTTTGGGAGCACAGGACACATTGTACGACCATTTGGGAAGGCATTATTACAAAGATTGTTACATTGAAGGTTCTGTTGATTTCATCTTCGGAAATGGTCTCTCTCTGTTTGAGGGATGTCACGTGCATGCAATAGCAACAAGCATAGGGGCATTAACGGCGCAGGGGAGGAGCAGTTTACTGGAGGAGACGGGGTTCTCGTTTGTCAACTGTAAGGTCACGGGTTCAGGAGCTCTCTATCTTGGAAGGGCTTGGGGACCTTTTTCTAGGGTAGTCTTTGCCTACACTTATATGGACAACATTATCACGCCCAAAGGCTGGTACAACTGGGGTGACCCAAATCGTGAAATGTACGCAACTCTCTCTCTCCCTATCTTGTTATGCATTGTATGTGTTTATGCTCAAAACGACATTGCTTTAGAACTTTATATCAAACTTTTTGAATCTAGACTCTTAGAAGCATTTCATCAGTACTTTATTCACGCTAACTTTCACTTTACACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

385

Amino Acids

42.84

Weight (kDa)

9.38

Isoelectric Point (pI)

31.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 85 - 329 2.7e-62 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 210, 401, 498
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 969
AccI GTMKAC 1 cut(s) 360
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 105, 383, 422
AcoI YGGCCR 2 cut(s) 92, 123
AcsI RAATTY 3 cut(s) 87, 288, 520
AcuI CTGAAG 1 cut(s) 450
AcvI CACGTG 1 cut(s) 775
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 6 cut(s) 117, 573, 677, 974, 1004, 1124
AfiI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 969
AgsI TTSAA 5 cut(s) 391, 440, 556, 719, 1094
AhdI GACNNNNNGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 120, 539, 580
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
AluBI AGCT 3 cut(s) 229, 455, 884
AluI AGCT 3 cut(s) 229, 455, 884
Alw21I GWGCWC 2 cut(s) 664, 886
Alw26I GTCTC 5 cut(s) 184, 270, 441, 755, 833
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 92, 123
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 3 cut(s) 87, 288, 520
Asp700I GAANNNNTTC 1 cut(s) 1113
Asp718I GGTACC 1 cut(s) 571
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 346, 470, 908
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 385, 516, 995
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 3 cut(s) 346, 470, 908
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 2 cut(s) 145, 886
BbrPI CACGTG 1 cut(s) 775
Bbv12I GWGCWC 2 cut(s) 664, 886
BbvI GCAGC 1 cut(s) 644
BccI CCATC 1 cut(s) 481
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 3 cut(s) 122, 541, 582
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 5 cut(s) 184, 270, 441, 755, 833
BfaI CTAG 2 cut(s) 918, 1098
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 1 cut(s) 633
BlsI GCNGC 1 cut(s) 634
BmcAI AGTACT 1 cut(s) 1124
Bme1390I CCNGG 4 cut(s) 96, 122, 541, 582
Bme18I GGWCC 3 cut(s) 346, 470, 908
BmeRI GACNNNNNGTC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 3 cut(s) 346, 470, 908
BmiI GGNNCC 3 cut(s) 573, 579, 909
BmrFI CCNGG 4 cut(s) 96, 122, 541, 582
BmrI ACTGGG 1 cut(s) 988
BmuI ACTGGG 1 cut(s) 988
BpmI CTGGAG 2 cut(s) 564, 854
BpuMI CCSGG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 81
BsaXI ACNNNNNCTCC 4 cut(s) 811, 830, 841, 860
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 969
Bse118I RCCGGY 2 cut(s) 568, 574
Bse1I ACTGG 2 cut(s) 837, 983
Bse3DI GCAATG 2 cut(s) 534, 1062
BseBI CCWGG 3 cut(s) 122, 541, 582
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 773
BseLI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 969
BseMI GCAATG 2 cut(s) 534, 1062
BseNI ACTGG 2 cut(s) 837, 983
BseRI GAGGAG 2 cut(s) 835, 851
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 644
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 2 cut(s) 94, 125
BshNI GGYRCC 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 664, 886
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 4 cut(s) 95, 108, 569, 575
BslFI GGGAC 2 cut(s) 505, 921
BslI CCNNNNNNNGG 6 cut(s) 151, 271, 272, 581, 642, 969
BsmAI GTCTC 5 cut(s) 184, 270, 441, 755, 833
BsmBI CGTCTC 1 cut(s) 833
BsmFI GGGAC 2 cut(s) 505, 921
BsnI GGCC 2 cut(s) 94, 125
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 3 cut(s) 145, 664, 886
BspACI CCGC 3 cut(s) 105, 383, 422
BspANI GGCC 2 cut(s) 94, 125
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 573, 579, 909
BspMI ACCTGC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 755
BsrDI GCAATG 2 cut(s) 534, 1062
BsrFI RCCGGY 2 cut(s) 568, 574
BsrI ACTGG 2 cut(s) 837, 983
BssAI RCCGGY 2 cut(s) 568, 574
BssECI CCNNGG 1 cut(s) 81
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 3 cut(s) 122, 541, 582
Bst4CI ACNGT 1 cut(s) 863
Bst6I CTCTTC 3 cut(s) 42, 236, 315
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 775
BstC8I GCNNGC 2 cut(s) 600, 780
BstDEI CTNAG 3 cut(s) 258, 640, 1105
BstEII GGTNACC 1 cut(s) 983
BstF5I GGATG 1 cut(s) 773
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 814
BstMAI GTCTC 5 cut(s) 184, 270, 441, 755, 833
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 3 cut(s) 512, 584, 809
BstNI CCWGG 3 cut(s) 122, 541, 582
BstNSI RCATGY 1 cut(s) 782
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 4 cut(s) 94, 120, 539, 580
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 644
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 94, 125
BtsCI GGATG 1 cut(s) 773
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 1 cut(s) 609
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 600, 780
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 2 cut(s) 568, 574
Cfr13I GGNCC 3 cut(s) 346, 470, 908
CseI GACGC 1 cut(s) 305
Csp6I GTAC 6 cut(s) 116, 572, 676, 973, 1003, 1123
CviAII CATG 4 cut(s) 133, 166, 350, 779
CviQI GTAC 6 cut(s) 116, 572, 676, 973, 1003, 1123
DdeI CTNAG 3 cut(s) 258, 640, 1105
DriI GACNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 92, 123
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 3 cut(s) 42, 236, 315
Eam1105I GACNNNNNGTC 1 cut(s) 72
EarI CTCTTC 3 cut(s) 42, 236, 315
EciI GGCGGA 2 cut(s) 94, 437
Ecl136II GAGCTC 1 cut(s) 884
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 145, 886
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 3 cut(s) 346, 470, 908
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 450
Eco72I CACGTG 1 cut(s) 775
Eco88I CYCGRG 1 cut(s) 138
Eco91I GGTNACC 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 908
EcoO65I GGTNACC 1 cut(s) 983
EcoRI GAATTC 2 cut(s) 87, 520
EcoRII CCWGG 3 cut(s) 120, 539, 580
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 1037
EcoT38I GRGCYC 2 cut(s) 145, 886
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 833
FaeI CATG 4 cut(s) 136, 169, 353, 782
FaqI GGGAC 2 cut(s) 505, 921
FatI CATG 4 cut(s) 132, 165, 349, 778
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 633
FokI GGATG 1 cut(s) 780
FriOI GRGCYC 2 cut(s) 145, 886
Fsp4HI GCNGC 1 cut(s) 633
FspBI CTAG 2 cut(s) 918, 1098
GlaI GCGC 1 cut(s) 813
GluI GCNGC 1 cut(s) 633
GsuI CTGGAG 2 cut(s) 564, 854
HaeIII GGCC 2 cut(s) 94, 125
HapII CCGG 4 cut(s) 95, 108, 569, 575
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 4 cut(s) 136, 169, 353, 782
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 5 cut(s) 192, 305, 333, 1094, 1101
HpaII CCGG 4 cut(s) 95, 108, 569, 575
HphI GGTGA 3 cut(s) 385, 516, 995
Hpy166II GTNNAC 4 cut(s) 118, 361, 830, 859
Hpy188I TCNGA 4 cut(s) 154, 332, 593, 743
Hpy188III TCNNGA 7 cut(s) 166, 293, 556, 621, 879, 995, 1098
Hpy8I GTNNAC 4 cut(s) 118, 361, 830, 859
HpyAV CCTTC 7 cut(s) 276, 404, 474, 646, 684, 713, 891
HpyCH4III ACNGT 1 cut(s) 863
HpyCH4IV ACGT 1 cut(s) 774
HpyCH4V TGCA 5 cut(s) 353, 506, 778, 782, 1035
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 584, 809
HpyF3I CTNAG 3 cut(s) 258, 640, 1105
HpySE526I ACGT 1 cut(s) 774
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 4 cut(s) 136, 169, 353, 782
HspAI GCGC 1 cut(s) 812
KpnI GGTACC 1 cut(s) 575
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 7 cut(s) 154, 413, 583, 584, 659, 822, 881
Lsp1109I GCAGC 1 cut(s) 644
MaeI CTAG 2 cut(s) 918, 1098
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 5 cut(s) 74, 710, 770, 868, 983
MboII GAAGA 7 cut(s) 29, 32, 35, 223, 232, 302, 730
MhlI GDGCHC 3 cut(s) 145, 664, 886
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 6 cut(s) 87, 146, 161, 288, 499, 520
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 3 cut(s) 186, 342, 1095
MnlI CCTC 4 cut(s) 247, 757, 813, 829
Mox20I TGGCCA 1 cut(s) 125
Mph1103I ATGCAT 1 cut(s) 1037
MroXI GAANNNNTTC 1 cut(s) 1113
MscI TGGCCA 1 cut(s) 125
MseI TTAA 1 cut(s) 806
MslI CAYNNNNRTG 3 cut(s) 354, 777, 941
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 4 cut(s) 95, 108, 569, 575
MspR9I CCNGG 4 cut(s) 96, 122, 541, 582
MvaI CCWGG 3 cut(s) 122, 541, 582
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 3 cut(s) 512, 584, 809
NciI CCSGG 1 cut(s) 96
NlaIII CATG 4 cut(s) 136, 169, 353, 782
NlaIV GGNNCC 3 cut(s) 573, 579, 909
NmuCI GTSAC 4 cut(s) 74, 770, 868, 983
NsiI ATGCAT 1 cut(s) 1037
NspI RCATGY 1 cut(s) 782
PaeI GCATGC 1 cut(s) 782
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 848
PdmI GAANNNNTTC 1 cut(s) 1113
PfeI GAWTC 2 cut(s) 305, 1094
PflMI CCANNNNNTGG 1 cut(s) 969
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 1 cut(s) 634
PleI GAGTC 3 cut(s) 186, 341, 1095
PmaCI CACGTG 1 cut(s) 775
PmlI CACGTG 1 cut(s) 775
PpsI GAGTC 3 cut(s) 186, 341, 1095
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 908
PsiI TTATAA 3 cut(s) 210, 401, 498
Psp124BI GAGCTC 1 cut(s) 886
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 3 cut(s) 120, 539, 580
PspCI CACGTG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 983
PspGI CCWGG 3 cut(s) 120, 539, 580
PspN4I GGNNCC 3 cut(s) 573, 579, 909
PspPI GGNCC 3 cut(s) 346, 470, 908
PspPPI RGGWCCY 1 cut(s) 908
RsaI GTAC 6 cut(s) 117, 573, 677, 974, 1004, 1124
RsaNI GTAC 6 cut(s) 116, 572, 676, 973, 1003, 1123
RseI CAYNNNNRTG 3 cut(s) 354, 777, 941
SacI GAGCTC 1 cut(s) 886
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 806
SatI GCNGC 1 cut(s) 633
Sau96I GGNCC 3 cut(s) 346, 470, 908
ScaI AGTACT 1 cut(s) 1124
SchI GAGTC 3 cut(s) 186, 342, 1095
ScrFI CCNGG 4 cut(s) 96, 122, 541, 582
SduI GDGCHC 3 cut(s) 145, 664, 886
SinI GGWCC 3 cut(s) 346, 470, 908
SmiMI CAYNNNNRTG 3 cut(s) 354, 777, 941
SphI GCATGC 1 cut(s) 782
Sse9I AATT 6 cut(s) 87, 146, 161, 288, 499, 520
SsiI CCGC 3 cut(s) 105, 383, 422
SspMI CTAG 2 cut(s) 918, 1098
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 94, 120, 539, 580
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 863
TaiI ACGT 1 cut(s) 777
TasI AATT 6 cut(s) 87, 146, 161, 288, 499, 520
TatI WGTACW 2 cut(s) 115, 1122
TfiI GAWTC 2 cut(s) 305, 1094
Tru1I TTAA 1 cut(s) 806
Tru9I TTAA 1 cut(s) 806
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 4 cut(s) 74, 770, 868, 983
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 4 cut(s) 74, 770, 868, 983
TspDTI ATGAA 6 cut(s) 154, 182, 307, 523, 724, 1106
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 1 cut(s) 609
Van91I CCANNNNNTGG 1 cut(s) 969
VpaK11BI GGWCC 3 cut(s) 346, 470, 908
XapI RAATTY 3 cut(s) 87, 288, 520
XbaI TCTAGA 1 cut(s) 1097
XceI RCATGY 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 1113
XspI CTAG 2 cut(s) 918, 1098
ZrmI AGTACT 1 cut(s) 1124
Zsp2I ATGCAT 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.