MD15G1197500.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009451
Physical Location & Seq
Reverse (-)
15689035 .. 15692504
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1197500.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGTCAACATTATTCAAACACATTTTCTATGTTCTTATTCTTCTTCTCCTCCTCAATTCAACCCCATCACAATGCCATTCCAAGGGACTCCGACCGGGGAAGCCAGACGGAAACGTGCACTTAACGAAGAACCTGACTCGAGCCCAATTATCAGAACAGCAATTCATGAAGTGGGTTAAGTTTGTTGGAAGCCTCAAACACTCTGTTTTCAAAGCAGCAAAGAACAAGCTGTTTCCTTCTTTCACTTTGCATGTGGCCAAGAACCCTAATGCAGGACACTTCACAAAAATCCAAGCCGCCATTGATTCTCTCCCCTTCATCAATCTCGTGAGAGTAGTCATCAAAGTACATGCAGGAGTATACATGGAGAAGGTAAATATACCTCCACTGAAATCATTTATAACCATAGAAGGAGCAGGGGCAGATAAAACCATTGTTCAATGGGGAGACACAGCTCAAACACCATCTGGTCCAAAAAAACAACCGATGGGGACTTTCAATTCTGCAACTTTTGCTGTGAATTCCCCTTATTTCATTGCCAAAAACATTACATTCAAGAACACTTCACCCGTCCCTAAACCTGGAGCAATTGGGAAGCAGGCAGTGGCATTTAGAATATCAGCAGATACAGCATCCTTCTTGGGGTGTAGATTTTTGGGGGCACAAGACACACTTTATGATCATTTGGGAAGGCATTATTACAAGGATTGCTACATTGAAGGTTCTGTGGATTTCATCTTTGGAAATGGACTCTCCCTATTTGAGGGATGTCACGTGCATGCAATAGCACAGAACATAGGAGCACTCACGGCGCAAGGCAGAAGCAGTATGTTGGAGGAAACGGGCTTCTCATTTGTAAACTGTAAGGTCACGGGTTCAGGAGCTCTCTATCTTGGTAGGGCTTGGGGTCCCTTTTCGAGGGTGGTCTTTGCGTACACATATATGGACAACATTATCATCCCTAAAGGCTGGTACAACTGGGGTGACCCTAATCGTGAAATGACTGTGTTTTATGGGCAATACAAGTGCACAGGACCAGGAGCTCGCTTTGCAGGGAGGGTTTCGTGGAGCAGAGAACTCACTAAAGAGGAAGCCAAGCCCTTCATTTCCCTTACCTTCATCGATGGCTCTGAGTGGATCAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

383

Amino Acids

42.29

Weight (kDa)

9.61

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 85 - 376 7.8e-73 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 401
AccI GTMKAC 1 cut(s) 360
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 1145
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 520
AcvI CACGTG 1 cut(s) 775
AfaI GTAC 3 cut(s) 348, 935, 974
AfiI CCNNNNNNNGG 6 cut(s) 82, 272, 581, 642, 763, 918
AgsI TTSAA 7 cut(s) 16, 60, 211, 440, 499, 556, 719
AjnI CCWGG 2 cut(s) 580, 1036
AluBI AGCT 4 cut(s) 229, 455, 884, 1043
AluI AGCT 4 cut(s) 229, 455, 884, 1043
Alw21I GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
Alw26I GTCTC 1 cut(s) 441
Alw44I GTGCAC 2 cut(s) 116, 1027
AlwI GGATC 1 cut(s) 1145
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 1 cut(s) 255
ApaLI GTGCAC 2 cut(s) 116, 1027
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 520
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 470, 908, 1034
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 558, 995
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 3 cut(s) 470, 908, 1034
BaeGI GKGCMC 3 cut(s) 120, 664, 1031
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 3 cut(s) 145, 886, 1045
BauI CACGAG 1 cut(s) 326
BbrPI CACGTG 1 cut(s) 775
Bbv12I GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
BbvI GCAGC 1 cut(s) 227
BccI CCATC 4 cut(s) 73, 472, 481, 1118
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 2 cut(s) 582, 1038
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 441
BisI GCNGC 2 cut(s) 216, 297
BlsI GCNGC 2 cut(s) 217, 298
Bme1390I CCNGG 3 cut(s) 96, 582, 1038
Bme18I GGWCC 3 cut(s) 470, 908, 1034
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 3 cut(s) 470, 908, 1034
BmiI GGNNCC 2 cut(s) 909, 910
BmrFI CCNGG 3 cut(s) 96, 582, 1038
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 988
BpmI CTGGAG 1 cut(s) 603
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 1122
BsaAI YACGTR 1 cut(s) 775
BsaJI CCNNGG 2 cut(s) 81, 95
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 272, 581, 642, 763, 918
Bse1I ACTGG 1 cut(s) 983
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 2 cut(s) 582, 1038
BseCI ATCGAT 1 cut(s) 1122
BseDI CCNNGG 2 cut(s) 81, 95
BseGI GGATG 3 cut(s) 632, 773, 957
BseLI CCNNNNNNNGG 6 cut(s) 82, 272, 581, 642, 763, 918
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 1 cut(s) 1122
BseNI ACTGG 1 cut(s) 983
BseRI GAGGAG 2 cut(s) 38, 41
BseSI GKGCMC 3 cut(s) 120, 664, 1031
BseXI GCAGC 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 1 cut(s) 257
BshVI ATCGAT 1 cut(s) 1122
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 4 cut(s) 99, 505, 557, 894
BslI CCNNNNNNNGG 6 cut(s) 82, 272, 581, 642, 763, 918
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 4 cut(s) 99, 505, 557, 894
BsnI GGCC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
Bsp143I GATC 2 cut(s) 679, 1137
BspACI CCGC 1 cut(s) 297
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 1 cut(s) 1123
BspDI ATCGAT 1 cut(s) 1122
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 909, 910
BspPI GGATC 1 cut(s) 1145
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 1 cut(s) 983
BssECI CCNNGG 2 cut(s) 81, 95
BssMI GATC 2 cut(s) 679, 1137
BssNAI GTATAC 1 cut(s) 361
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 361
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 582, 1038
Bst4CI ACNGT 2 cut(s) 863, 1006
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 775
BstC8I GCNNGC 3 cut(s) 600, 780, 1045
BstDEI CTNAG 1 cut(s) 1131
BstEII GGTNACC 1 cut(s) 983
BstENI CCTNNNNNAGG 3 cut(s) 270, 761, 916
BstF5I GGATG 3 cut(s) 632, 773, 957
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 2 cut(s) 682, 1140
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 2 cut(s) 679, 1137
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
BstNI CCWGG 2 cut(s) 582, 1038
BstNSI RCATGY 3 cut(s) 254, 353, 782
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 3 cut(s) 94, 580, 1036
BstSLI GKGCMC 3 cut(s) 120, 664, 1031
BstV1I GCAGC 1 cut(s) 227
BstZ17I GTATAC 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 1122
BsuRI GGCC 1 cut(s) 257
BsuTUI ATCGAT 1 cut(s) 1122
BtsCI GGATG 3 cut(s) 632, 773, 957
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 386, 609
Cac8I GCNNGC 3 cut(s) 600, 780, 1045
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 3 cut(s) 470, 908, 1034
ClaI ATCGAT 1 cut(s) 1122
Csp6I GTAC 3 cut(s) 347, 934, 973
CviAII CATG 5 cut(s) 166, 251, 350, 364, 779
CviQI GTAC 3 cut(s) 347, 934, 973
DdeI CTNAG 1 cut(s) 1131
DpnI GATC 2 cut(s) 681, 1139
DpnII GATC 2 cut(s) 679, 1137
EaeI YGGCCR 1 cut(s) 255
Ecl136II GAGCTC 2 cut(s) 884, 1043
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 3 cut(s) 145, 886, 1045
Eco47I GGWCC 3 cut(s) 470, 908, 1034
Eco53kI GAGCTC 2 cut(s) 884, 1043
Eco72I CACGTG 1 cut(s) 775
Eco88I CYCGRG 1 cut(s) 138
Eco91I GGTNACC 1 cut(s) 983
EcoICRI GAGCTC 2 cut(s) 884, 1043
EcoNI CCTNNNNNAGG 3 cut(s) 270, 761, 916
EcoO109I RGGNCCY 1 cut(s) 908
EcoO65I GGTNACC 1 cut(s) 983
EcoRI GAATTC 1 cut(s) 520
EcoRII CCWGG 2 cut(s) 580, 1036
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 3 cut(s) 145, 886, 1045
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 169, 254, 353, 367, 782
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 4 cut(s) 99, 505, 557, 894
FatI CATG 5 cut(s) 165, 250, 349, 363, 778
FbaI TGATCA 1 cut(s) 679
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 216, 297
FokI GGATG 3 cut(s) 619, 780, 944
FriOI GRGCYC 3 cut(s) 145, 886, 1045
Fsp4HI GCNGC 2 cut(s) 216, 297
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 216, 297
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 95
HhaI GCGC 1 cut(s) 814
Hin1II CATG 5 cut(s) 169, 254, 353, 367, 782
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 87, 136, 305, 750
HpaII CCGG 1 cut(s) 95
HphI GGTGA 2 cut(s) 558, 995
Hpy166II GTNNAC 6 cut(s) 6, 118, 361, 859, 936, 1029
Hpy188I TCNGA 3 cut(s) 92, 154, 1132
Hpy188III TCNNGA 5 cut(s) 166, 328, 556, 879, 995
Hpy8I GTNNAC 6 cut(s) 6, 118, 361, 859, 936, 1029
HpyAV CCTTC 9 cut(s) 246, 325, 364, 404, 646, 684, 713, 1111, 1126
HpyCH4III ACNGT 2 cut(s) 863, 1006
HpyCH4IV ACGT 2 cut(s) 114, 774
HpyCH4V TGCA 9 cut(s) 118, 250, 272, 353, 506, 778, 782, 1029, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
HpyF3I CTNAG 1 cut(s) 1131
HpySE526I ACGT 2 cut(s) 114, 774
Hsp92II CATG 5 cut(s) 169, 254, 353, 367, 782
HspAI GCGC 1 cut(s) 812
KflI GGGWCCC 1 cut(s) 908
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 2 cut(s) 679, 1137
LmnI GCTCC 6 cut(s) 413, 584, 800, 881, 1040, 1068
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 641
MaeII ACGT 2 cut(s) 114, 774
MaeIII GTNAC 3 cut(s) 770, 868, 983
MalI GATC 2 cut(s) 681, 1139
MboI GATC 2 cut(s) 679, 1137
MboII GAAGA 3 cut(s) 32, 35, 139
MfeI CAATTG 1 cut(s) 588
MhlI GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 6 cut(s) 55, 146, 161, 499, 520, 588
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 3 cut(s) 81, 130, 744
MmeI TCCRAC 3 cut(s) 115, 166, 813
MnlI CCTC 9 cut(s) 59, 62, 203, 393, 757, 829, 912, 1050, 1081
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 2 cut(s) 122, 177
MslI CAYNNNNRTG 3 cut(s) 70, 777, 941
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 3 cut(s) 96, 582, 1038
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 2 cut(s) 582, 1038
MwoI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
NciI CCSGG 1 cut(s) 96
NdeII GATC 2 cut(s) 679, 1137
NlaIII CATG 5 cut(s) 169, 254, 353, 367, 782
NlaIV GGNNCC 2 cut(s) 909, 910
NmuCI GTSAC 3 cut(s) 770, 868, 983
NspI RCATGY 3 cut(s) 254, 353, 782
PaeI GCATGC 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 2 cut(s) 217, 298
PleI GAGTC 3 cut(s) 81, 130, 744
PmaCI CACGTG 1 cut(s) 775
PmlI CACGTG 1 cut(s) 775
PpsI GAGTC 3 cut(s) 81, 130, 744
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 908
PsiI TTATAA 1 cut(s) 401
Psp124BI GAGCTC 2 cut(s) 886, 1045
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 2 cut(s) 580, 1036
PspCI CACGTG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 983
PspGI CCWGG 2 cut(s) 580, 1036
PspN4I GGNNCC 2 cut(s) 909, 910
PspPI GGNCC 3 cut(s) 470, 908, 1034
PspPPI RGGWCCY 1 cut(s) 908
PspXI VCTCGAGB 1 cut(s) 138
RsaI GTAC 3 cut(s) 348, 935, 974
RsaNI GTAC 3 cut(s) 347, 934, 973
RseI CAYNNNNRTG 3 cut(s) 70, 777, 941
SacI GAGCTC 2 cut(s) 886, 1045
SaqAI TTAA 2 cut(s) 122, 177
SatI GCNGC 2 cut(s) 216, 297
Sau3AI GATC 2 cut(s) 679, 1137
Sau96I GGNCC 3 cut(s) 470, 908, 1034
SchI GAGTC 3 cut(s) 81, 130, 744
ScrFI CCNGG 3 cut(s) 96, 582, 1038
SduI GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
SfaNI GCATC 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 138
SinI GGWCC 3 cut(s) 470, 908, 1034
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 3 cut(s) 70, 777, 941
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SphI GCATGC 1 cut(s) 782
Sse9I AATT 6 cut(s) 55, 146, 161, 499, 520, 588
SsiI CCGC 1 cut(s) 297
SstI GAGCTC 2 cut(s) 886, 1045
StyD4I CCNGG 3 cut(s) 94, 580, 1036
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 863, 1006
TaiI ACGT 2 cut(s) 117, 777
TaqI TCGA 3 cut(s) 139, 917, 1122
TasI AATT 6 cut(s) 55, 146, 161, 499, 520, 588
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 2 cut(s) 122, 177
Tru9I TTAA 2 cut(s) 122, 177
TscAI CASTG 2 cut(s) 393, 609
TseFI GTSAC 3 cut(s) 770, 868, 983
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 3 cut(s) 770, 868, 983
TspDTI ATGAA 7 cut(s) 154, 182, 307, 523, 724, 1093, 1108
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 2 cut(s) 393, 609
VneI GTGCAC 2 cut(s) 116, 1027
VpaK11BI GGWCC 3 cut(s) 470, 908, 1034
XagI CCTNNNNNAGG 3 cut(s) 270, 761, 916
XapI RAATTY 1 cut(s) 520
XceI RCATGY 3 cut(s) 254, 353, 782
XhoI CTCGAG 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.