MD02G1067000.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009451
Physical Location & Seq
Reverse (-)
5479905 .. 5483390
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1067000.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGTCAACATTATTCCAGCACATTTTCTATGTTCTTATTCTTCTTCTCCTCCTCTATTCAACCCCATCACAGTGCCATTCCAAGGGACTCCGACCGGGAAATCCGGCCGGAAACGTGCACTTAACGAAGAACATGACTCGAGCCCAATTATCGGAACAGCAATTTATGAAGTGGGTTAAGTTTGTTGGAAGCCTCAAACACTCTGTTTTCAAAGCAGCAAAGAACAAGCTCTCTCCTTCTTCCACACTGCATGTGGCCAAGAACCCTAATGCAGGAGACTTCACAAAAATCCAAGACGCCATTGATTCTCTCCCCTTCATCAATCTCGTGAGAGTTGTCATCAAAGTACACGCAGGAATATACACGGAGAAGGTAAATATACCTCCATTGAAATCATTTATAACCATAGAAGGAGCAGGGGCAGATAAAACCATTGTTCAATGGGGAGACACAGCTCAAACACCATCTGGTCCAAAAAAACAACCCATGGGAACTTTTAATTCTGCAACTTTTGCTGTCAATTCCCCTTATTTTATTGCCAGAAACATTACATTTAAGAACACTACACCAGTCCCCAAACCAGGAGCAATTGGGAAGCAGGCAGTGGCGTTTAGAATATCAGCTGATACAGCAACTTTCTGGGGGTGTAGTTTTTTGGGGGCACAAGATACACTCTATGATCATTTGGGAAGGCATTATTACAAGGATTGCTATATTGAAGGTTCTGTGGATTTCATCTTTGGAAATGGACTCTCCCTATTTGAGGGATGTCACGTGCATGCAATAGCACAGAACATAGGAGCACTCACGGCGCAAGGCAGAAGCAGTATGTTGGAGGAAACGGGCTTCTCATTTGTAAACTGTAAGGTCACGGGTTCAGGAGCTCTCTATCTTGGTAGGGCTTGGGGTCCCTTCTCCAGGGTGGTCTTTGCCTACACATACATGGACGATATTATCATCCCTAAAGGCTGGTACAACTGGGGTGACCCTAATCGTGAAATGACTGTGTTTTATGGGCAATACAAGTGCACAGGACCAGGAGCTCGCTTTGCAGGGAGGGTTTCGTGGAGCAGAGAACTCACTGATGAGGAAGCCAAGCCCTTTATTTCCCTTACCTTCATCGATGGCTCTGAGTGGATCAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

383

Amino Acids

42.23

Weight (kDa)

9.27

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 85 - 376 5.3e-74 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 401
AclWI GGATC 1 cut(s) 1145
AcoI YGGCCR 2 cut(s) 105, 255
AcvI CACGTG 1 cut(s) 775
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 348, 974
AfiI CCNNNNNNNGG 6 cut(s) 82, 151, 272, 581, 763, 918
AgsI TTSAA 5 cut(s) 60, 211, 391, 440, 719
AjnI CCWGG 3 cut(s) 580, 917, 1036
AluBI AGCT 5 cut(s) 229, 455, 623, 884, 1043
AluI AGCT 5 cut(s) 229, 455, 623, 884, 1043
Alw21I GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
Alw26I GTCTC 2 cut(s) 270, 441
Alw44I GTGCAC 2 cut(s) 116, 1027
AlwI GGATC 1 cut(s) 1145
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 105, 255
ApaLI GTGCAC 2 cut(s) 116, 1027
ApeKI GCWGC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 470, 908, 1034
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 995
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 3 cut(s) 470, 908, 1034
BaeGI GKGCMC 3 cut(s) 120, 664, 1031
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 3 cut(s) 145, 886, 1045
BauI CACGAG 1 cut(s) 326
BbrPI CACGTG 1 cut(s) 775
Bbv12I GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
BbvI GCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 73, 472, 1118
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 3 cut(s) 582, 919, 1038
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 270, 441
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 4 cut(s) 96, 582, 919, 1038
Bme18I GGWCC 3 cut(s) 470, 908, 1034
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 3 cut(s) 470, 908, 1034
BmiI GGNNCC 2 cut(s) 909, 910
BmrFI CCNGG 4 cut(s) 96, 582, 919, 1038
BmrI ACTGGG 1 cut(s) 988
BmuI ACTGGG 1 cut(s) 988
BpmI CTGGAG 1 cut(s) 901
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 1122
BsaAI YACGTR 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 81, 486, 918
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 151, 272, 581, 763, 918
Bse1I ACTGG 2 cut(s) 569, 983
BseBI CCWGG 3 cut(s) 582, 919, 1038
BseCI ATCGAT 1 cut(s) 1122
BseDI CCNNGG 3 cut(s) 81, 486, 918
BseGI GGATG 2 cut(s) 773, 957
BseLI CCNNNNNNNGG 6 cut(s) 82, 151, 272, 581, 763, 918
BseMII CTCAG 1 cut(s) 1122
BseNI ACTGG 2 cut(s) 569, 983
BseRI GAGGAG 2 cut(s) 38, 41
BseSI GKGCMC 3 cut(s) 120, 664, 1031
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 227
Bsh1285I CGRYCG 2 cut(s) 95, 108
BshFI GGCC 2 cut(s) 107, 257
BshVI ATCGAT 1 cut(s) 1122
BsiEI CGRYCG 2 cut(s) 95, 108
BsiHKAI GWGCWC 5 cut(s) 120, 805, 886, 1031, 1045
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 3 cut(s) 95, 104, 108
BslFI GGGAC 3 cut(s) 99, 557, 894
BslI CCNNNNNNNGG 6 cut(s) 82, 151, 272, 581, 763, 918
BsmAI GTCTC 2 cut(s) 270, 441
BsmFI GGGAC 3 cut(s) 99, 557, 894
BsnI GGCC 2 cut(s) 107, 257
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
Bsp143I GATC 2 cut(s) 679, 1137
Bsp19I CCATGG 1 cut(s) 486
BspANI GGCC 2 cut(s) 107, 257
BspCNI CTCAG 1 cut(s) 1123
BspDI ATCGAT 1 cut(s) 1122
BspLI GGNNCC 2 cut(s) 909, 910
BspPI GGATC 1 cut(s) 1145
BsrI ACTGG 2 cut(s) 569, 983
BssECI CCNNGG 3 cut(s) 81, 486, 918
BssMI GATC 2 cut(s) 679, 1137
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 2 cut(s) 81, 486
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 3 cut(s) 582, 919, 1038
Bst4CI ACNGT 3 cut(s) 72, 863, 1006
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 775
BstC8I GCNNGC 3 cut(s) 600, 780, 1045
BstDEI CTNAG 1 cut(s) 1131
BstDSI CCRYGG 1 cut(s) 486
BstEII GGTNACC 1 cut(s) 983
BstENI CCTNNNNNAGG 3 cut(s) 270, 761, 916
BstF5I GGATG 2 cut(s) 773, 957
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 2 cut(s) 682, 1140
BstMAI GTCTC 2 cut(s) 270, 441
BstMBI GATC 2 cut(s) 679, 1137
BstMCI CGRYCG 2 cut(s) 95, 108
BstMWI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
BstNI CCWGG 3 cut(s) 582, 919, 1038
BstNSI RCATGY 2 cut(s) 254, 782
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 4 cut(s) 94, 580, 917, 1036
BstSLI GKGCMC 3 cut(s) 120, 664, 1031
BstV1I GCAGC 1 cut(s) 227
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 1122
BsuRI GGCC 2 cut(s) 107, 257
BsuTUI ATCGAT 1 cut(s) 1122
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 773, 957
BtsI GCAGTG 2 cut(s) 245, 609
BtsIMutI CAGTG 4 cut(s) 77, 245, 609, 1080
Cac8I GCNNGC 3 cut(s) 600, 780, 1045
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 3 cut(s) 470, 908, 1034
ClaI ATCGAT 1 cut(s) 1122
CseI GACGC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 347, 973
CviAII CATG 5 cut(s) 133, 251, 487, 779, 943
CviQI GTAC 2 cut(s) 347, 973
DdeI CTNAG 1 cut(s) 1131
DpnI GATC 2 cut(s) 681, 1139
DpnII GATC 2 cut(s) 679, 1137
EaeI YGGCCR 2 cut(s) 105, 255
EagI CGGCCG 1 cut(s) 105
Ecl136II GAGCTC 2 cut(s) 884, 1043
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 81, 486
Eco24I GRGCYC 3 cut(s) 145, 886, 1045
Eco47I GGWCC 3 cut(s) 470, 908, 1034
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 2 cut(s) 884, 1043
Eco72I CACGTG 1 cut(s) 775
Eco88I CYCGRG 1 cut(s) 138
Eco91I GGTNACC 1 cut(s) 983
EcoICRI GAGCTC 2 cut(s) 884, 1043
EcoNI CCTNNNNNAGG 3 cut(s) 270, 761, 916
EcoO109I RGGNCCY 1 cut(s) 908
EcoO65I GGTNACC 1 cut(s) 983
EcoRII CCWGG 3 cut(s) 580, 917, 1036
EcoT14I CCWWGG 2 cut(s) 81, 486
EcoT38I GRGCYC 3 cut(s) 145, 886, 1045
ErhI CCWWGG 2 cut(s) 81, 486
FaeI CATG 5 cut(s) 136, 254, 490, 782, 946
FaqI GGGAC 3 cut(s) 99, 557, 894
FatI CATG 5 cut(s) 132, 250, 486, 778, 942
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 780, 944
FriOI GRGCYC 3 cut(s) 145, 886, 1045
Fsp4HI GCNGC 1 cut(s) 216
GlaI GCGC 1 cut(s) 813
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 901
HaeIII GGCC 2 cut(s) 107, 257
HapII CCGG 3 cut(s) 95, 104, 108
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 136, 254, 490, 782, 946
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 87, 136, 305, 750
HpaII CCGG 3 cut(s) 95, 104, 108
HphI GGTGA 1 cut(s) 995
Hpy166II GTNNAC 5 cut(s) 6, 118, 349, 859, 1029
Hpy188I TCNGA 3 cut(s) 92, 154, 1132
Hpy188III TCNNGA 3 cut(s) 328, 879, 995
Hpy8I GTNNAC 5 cut(s) 6, 118, 349, 859, 1029
HpyAV CCTTC 8 cut(s) 246, 325, 364, 404, 684, 713, 922, 1126
HpyCH4III ACNGT 3 cut(s) 72, 863, 1006
HpyCH4IV ACGT 2 cut(s) 114, 774
HpyCH4V TGCA 8 cut(s) 118, 250, 272, 506, 778, 782, 1029, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
HpyF3I CTNAG 1 cut(s) 1131
HpySE526I ACGT 2 cut(s) 114, 774
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 136, 254, 490, 782, 946
HspAI GCGC 1 cut(s) 812
KflI GGGWCCC 1 cut(s) 908
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 2 cut(s) 679, 1137
LmnI GCTCC 6 cut(s) 413, 584, 800, 881, 1040, 1068
Lsp1109I GCAGC 1 cut(s) 227
MaeII ACGT 2 cut(s) 114, 774
MaeIII GTNAC 3 cut(s) 770, 868, 983
MalI GATC 2 cut(s) 681, 1139
MboI GATC 2 cut(s) 679, 1137
MboII GAAGA 4 cut(s) 32, 35, 139, 231
MfeI CAATTG 1 cut(s) 588
MhlI GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 5 cut(s) 146, 161, 499, 520, 588
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 3 cut(s) 81, 130, 744
MmeI TCCRAC 3 cut(s) 115, 166, 813
MnlI CCTC 8 cut(s) 59, 62, 203, 393, 757, 829, 1050, 1081
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 4 cut(s) 122, 177, 498, 555
MslI CAYNNNNRTG 3 cut(s) 70, 777, 941
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 3 cut(s) 95, 104, 108
MspR9I CCNGG 4 cut(s) 96, 582, 919, 1038
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 3 cut(s) 582, 919, 1038
MwoI GCNNNNNNNGC 4 cut(s) 512, 629, 809, 1049
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 1 cut(s) 486
NdeII GATC 2 cut(s) 679, 1137
NlaIII CATG 5 cut(s) 136, 254, 490, 782, 946
NlaIV GGNNCC 2 cut(s) 909, 910
NmuCI GTSAC 3 cut(s) 770, 868, 983
NspI RCATGY 2 cut(s) 254, 782
PaeI GCATGC 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 138
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 3 cut(s) 81, 130, 744
PmaCI CACGTG 1 cut(s) 775
PmlI CACGTG 1 cut(s) 775
PpsI GAGTC 3 cut(s) 81, 130, 744
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 908
PsiI TTATAA 1 cut(s) 401
Psp124BI GAGCTC 2 cut(s) 886, 1045
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 3 cut(s) 580, 917, 1036
PspCI CACGTG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 983
PspGI CCWGG 3 cut(s) 580, 917, 1036
PspN4I GGNNCC 2 cut(s) 909, 910
PspPI GGNCC 3 cut(s) 470, 908, 1034
PspPPI RGGWCCY 1 cut(s) 908
PspXI VCTCGAGB 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 348, 974
RsaNI GTAC 2 cut(s) 347, 973
RseI CAYNNNNRTG 3 cut(s) 70, 777, 941
SacI GAGCTC 2 cut(s) 886, 1045
SaqAI TTAA 4 cut(s) 122, 177, 498, 555
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 679, 1137
Sau96I GGNCC 3 cut(s) 470, 908, 1034
SchI GAGTC 3 cut(s) 81, 130, 744
ScrFI CCNGG 4 cut(s) 96, 582, 919, 1038
SduI GDGCHC 7 cut(s) 120, 145, 664, 805, 886, 1031, 1045
Sfr274I CTCGAG 1 cut(s) 138
SinI GGWCC 3 cut(s) 470, 908, 1034
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 3 cut(s) 70, 777, 941
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SphI GCATGC 1 cut(s) 782
Sse9I AATT 5 cut(s) 146, 161, 499, 520, 588
SstI GAGCTC 2 cut(s) 886, 1045
StyD4I CCNGG 4 cut(s) 94, 580, 917, 1036
StyI CCWWGG 2 cut(s) 81, 486
TaaI ACNGT 3 cut(s) 72, 863, 1006
TaiI ACGT 2 cut(s) 117, 777
TaqI TCGA 2 cut(s) 139, 1122
TasI AATT 5 cut(s) 146, 161, 499, 520, 588
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 4 cut(s) 122, 177, 498, 555
Tru9I TTAA 4 cut(s) 122, 177, 498, 555
TscAI CASTG 4 cut(s) 77, 252, 609, 1087
TseFI GTSAC 3 cut(s) 770, 868, 983
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 3 cut(s) 770, 868, 983
TspDTI ATGAA 4 cut(s) 182, 307, 724, 1108
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 4 cut(s) 77, 252, 609, 1087
VneI GTGCAC 2 cut(s) 116, 1027
VpaK11BI GGWCC 3 cut(s) 470, 908, 1034
XagI CCTNNNNNAGG 3 cut(s) 270, 761, 916
XceI RCATGY 2 cut(s) 254, 782
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.