AT5G20220

Zinc knuckle (CCHC-type) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013263
Physical Location & Seq
Forward (+)
6821881 .. 6825981
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20220.2

Sequence Viewer

Length: 1182 bp
ATGAGAAAGAAGAAGACCCGATTTCAGTTCCTTCTCAATCCCAACTCAAAATTCGTCTTTTTTCCTCGCGCGATTTCTTCTTCTTCTTCTTCTTCTTCCGACAACAACAACGATGGTTCAGTTTCATCTTCCAGACAAAACAACCGACAGATGGGTTATGATCCTTCAGAGGAACTCTTTGGTGTTGATTTCAAGCCCAGGTTCATATCTGGTGATTCGCGTGAACCAAGGTCATGGTTTGGTCCTAACGGTCAGTACATTCGAGAGCTTCCTTGTCCAACTTGTAGGGGAAGAGGTTATACTTCATGTTCAAATTGCGGAATCGAGAGGTCGCGATTAGACTGCCCTCAATGCAAAGGAAAGGGCATAATGACTTGTCTCAGATGTTTGGGGGATTGTGTCATATGGGAAGAATCAATCGATGAACGACCTTGGGAGAAAGCTAGATCCAGTTCTCCATTTAGAGTAAAGGAGGATGATGAGGTTGATAATTTAGAGATAAAGTTCAGTAAAAGGCGAAAATCGAAGCGGATTTATCAGTCACCAACTCCTGAAGTTGGACAAAAGATCAGCCGATCTCTAAAAAGTCTCAATGCCAAAACTGGACTTTTTAGTAAGCGCATGAAGATTATACATCGTGATCCAGTGCTTCATGCTCAAAGAGTAGCTGCAATTAAGAAAGCTAAAGGAACACCAGCTGCTAGAAAGCATGCATCCGAATCTATGAAAGCTTTCTTCAGTAACCCTGTAAACCGTGAACAGAGAAGCCTATCTATGAAAGGAACTAAGTTTTACTGCAAGAACTGTGGACAGGAAGGCCATAGACGCCATTACTGTCCAGAACTAGGCACTAATGCGGACAGAAAATTTAGATGTCGGGGTTGCGGCGGGAAAGGCCACAACCGTAGAACTTGTCCTAAGTCGAAATCAATAGTCACTAAAGGCATTTCCACTAGGTATCACAAATGTGGAATATGCGGTGAGAGAGGTCACAACAGCAGAACATGCCGGAAGCCGACCGGAGTGAATCCTAGTTGCAGTGGTGAAAATTCCGGGGAAGATGGTGTTGGTAAAATAACGTATGCTTGTGGGTTCTGCAAGAAAATGGGACATAATGTAAGAACCTGTCCAAGTAAACAAGTTTCAGACAGTGATTCTTGTTTAGAGCAAGAAGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_2

Protein Analysis

393

Amino Acids

44.11

Weight (kDa)

9.78

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20220 AT5G20220 AT5G20220 AT5G20220
fragaria_vesca FvH4_4g31851
malus_domestica MD13G1078100.v1.1
prunus_persica Prupe.1G269000_v2.0.a1
pyrus_communis pycom13g06760
rosa_chinensis RchiOBHm_Chr4g0440521
rosa_laevigata RLG00000006172
rosa_roxburghii Rroxscaffold_5G00381350
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378100 Rh4BG390700 Rh4CG405400 Rh4DG384400
rosa_wichuraiana Rw4G032360 Rw4G032460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 69, 71, 220, 334
AciI CCGC 6 cut(s) 318, 529, 857, 885, 888, 978
AclWI GGATC 3 cut(s) 155, 441, 635
AcsI RAATTY 3 cut(s) 50, 866, 1048
AcuI CTGAAG 3 cut(s) 150, 573, 721
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 151, 557, 845
AgsI TTSAA 2 cut(s) 193, 312
AjnI CCWGG 1 cut(s) 197
AjuI GAANNNNNNNTTGG 4 cut(s) 35, 67, 1050, 1082
AleI CACNNNNGTG 1 cut(s) 966
AluBI AGCT 6 cut(s) 268, 443, 668, 683, 698, 731
AluI AGCT 6 cut(s) 268, 443, 668, 683, 698, 731
Alw26I GTCTC 2 cut(s) 383, 593
AlwI GGATC 3 cut(s) 155, 441, 635
AoxI GGCC 2 cut(s) 817, 895
ApeKI GCWGC 2 cut(s) 668, 698
ApoI RAATTY 3 cut(s) 50, 866, 1048
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 2 cut(s) 71, 621
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 1054
AsuHPI GGTGA 4 cut(s) 224, 534, 992, 1055
AvaII GGWCC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 283, 315
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 655, 685
BccI CCATC 3 cut(s) 107, 145, 1055
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BciT130I CCWGG 1 cut(s) 199
BcnI CCSGG 1 cut(s) 1054
BcoDI GTCTC 2 cut(s) 383, 593
BfaI CTAG 5 cut(s) 444, 702, 845, 954, 1032
BisI GCNGC 3 cut(s) 669, 699, 886
BlsI GCNGC 3 cut(s) 670, 700, 887
Bme1390I CCNGG 2 cut(s) 199, 1054
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmrFI CCNGG 2 cut(s) 199, 1054
BmsI GCATC 1 cut(s) 722
BpiI GAAGAC 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 1054
Bsa29I ATCGAT 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 826
BsaJI CCNNGG 4 cut(s) 197, 227, 431, 1053
BsaWI WCCGGW 1 cut(s) 1019
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 557, 845
Bse1I ACTGG 3 cut(s) 450, 607, 644
BseBI CCWGG 1 cut(s) 199
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 197, 227, 431, 1053
BseGI GGATG 2 cut(s) 481, 713
BseLI CCNNNNNNNGG 3 cut(s) 151, 557, 845
BseMII CTCAG 1 cut(s) 394
BseNI ACTGG 3 cut(s) 450, 607, 644
BseXI GCAGC 2 cut(s) 655, 685
Bsh1236I CGCG 4 cut(s) 69, 71, 220, 334
Bsh1285I CGRYCG 1 cut(s) 1020
BshFI GGCC 2 cut(s) 819, 897
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 1 cut(s) 1020
BsiSI CCGG 3 cut(s) 1009, 1020, 1053
BslFI GGGAC 1 cut(s) 1122
BslI CCNNNNNNNGG 3 cut(s) 151, 557, 845
BsmAI GTCTC 2 cut(s) 383, 593
BsmFI GGGAC 1 cut(s) 1122
BsnI GGCC 2 cut(s) 819, 897
Bsp143I GATC 5 cut(s) 160, 446, 567, 575, 640
Bsp68I TCGCGA 1 cut(s) 334
BspACI CCGC 6 cut(s) 318, 529, 857, 885, 888, 978
BspANI GGCC 2 cut(s) 819, 897
BspCNI CTCAG 1 cut(s) 393
BspDI ATCGAT 1 cut(s) 420
BspFNI CGCG 4 cut(s) 69, 71, 220, 334
BspPI GGATC 3 cut(s) 155, 441, 635
BsrI ACTGG 3 cut(s) 450, 607, 644
BssECI CCNNGG 4 cut(s) 197, 227, 431, 1053
BssMI GATC 5 cut(s) 160, 446, 567, 575, 640
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 2 cut(s) 227, 431
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 6 cut(s) 251, 755, 806, 836, 905, 1151
Bst6I CTCTTC 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 826
BstAPI GCANNNNNTGC 1 cut(s) 1005
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 3 cut(s) 380, 786, 918
BstF5I GGATG 2 cut(s) 481, 713
BstFNI CGCG 4 cut(s) 69, 71, 220, 334
BstHHI GCGC 2 cut(s) 71, 621
BstKTI GATC 5 cut(s) 163, 449, 570, 578, 643
BstMAI GTCTC 2 cut(s) 383, 593
BstMBI GATC 5 cut(s) 160, 446, 567, 575, 640
BstMCI CGRYCG 1 cut(s) 1020
BstMWI GCNNNNNNNGC 4 cut(s) 351, 825, 894, 1005
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 713, 1008
BstSCI CCNGG 2 cut(s) 197, 1052
BstUI CGCG 4 cut(s) 69, 71, 220, 334
BstV1I GCAGC 2 cut(s) 655, 685
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 2 cut(s) 819, 897
BsuTUI ATCGAT 1 cut(s) 420
BtsCI GGATG 2 cut(s) 481, 713
BtsI GCAGTG 1 cut(s) 1045
BtsIMutI CAGTG 3 cut(s) 651, 1045, 1156
BtuMI TCGCGA 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 711
CfoI GCGC 2 cut(s) 71, 621
Cfr13I GGNCC 1 cut(s) 242
ClaI ATCGAT 1 cut(s) 420
CseI GACGC 1 cut(s) 834
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviAII CATG 6 cut(s) 234, 306, 622, 653, 710, 1005
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 3 cut(s) 380, 786, 918
DpnI GATC 5 cut(s) 162, 448, 569, 577, 642
DpnII GATC 5 cut(s) 160, 446, 567, 575, 640
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 227, 431
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 3 cut(s) 150, 573, 721
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 2 cut(s) 227, 431
EcoT22I ATGCAT 1 cut(s) 715
ErhI CCWWGG 2 cut(s) 227, 431
FaeI CATG 6 cut(s) 237, 309, 625, 656, 713, 1008
FaqI GGGAC 1 cut(s) 1122
FatI CATG 6 cut(s) 233, 305, 621, 652, 709, 1004
FauI CCCGC 1 cut(s) 881
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 3 cut(s) 669, 699, 886
FokI GGATG 2 cut(s) 488, 700
Fsp4HI GCNGC 3 cut(s) 669, 699, 886
FspBI CTAG 5 cut(s) 444, 702, 845, 954, 1032
GlaI GCGC 2 cut(s) 70, 620
GluI GCNGC 3 cut(s) 669, 699, 886
HaeIII GGCC 2 cut(s) 819, 897
HapII CCGG 3 cut(s) 1009, 1020, 1053
HgaI GACGC 1 cut(s) 834
HhaI GCGC 2 cut(s) 71, 621
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 6 cut(s) 237, 309, 625, 656, 713, 1008
Hin6I GCGC 2 cut(s) 69, 619
HinP1I GCGC 2 cut(s) 69, 619
HindIII AAGCTT 1 cut(s) 729
HinfI GANTC 6 cut(s) 215, 321, 413, 719, 1027, 1154
HpaII CCGG 3 cut(s) 1009, 1020, 1053
HphI GGTGA 4 cut(s) 224, 534, 992, 1055
Hpy166II GTNNAC 5 cut(s) 224, 751, 758, 809, 1136
Hpy188I TCNGA 5 cut(s) 100, 169, 383, 718, 1147
Hpy188III TCNNGA 8 cut(s) 132, 263, 325, 333, 551, 638, 839, 1179
Hpy8I GTNNAC 5 cut(s) 224, 751, 758, 809, 1136
HpyAV CCTTC 4 cut(s) 41, 174, 809, 1166
HpyCH4III ACNGT 6 cut(s) 251, 755, 806, 836, 905, 1151
HpyCH4IV ACGT 1 cut(s) 1079
HpyCH4V TGCA 6 cut(s) 354, 671, 713, 798, 1038, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 351, 825, 894, 1005
HpyF3I CTNAG 3 cut(s) 380, 786, 918
HpySE526I ACGT 1 cut(s) 1079
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 6 cut(s) 237, 309, 625, 656, 713, 1008
HspAI GCGC 2 cut(s) 69, 619
Kzo9I GATC 5 cut(s) 160, 446, 567, 575, 640
Lsp1109I GCAGC 2 cut(s) 655, 685
LweI GCATC 1 cut(s) 722
MaeI CTAG 5 cut(s) 444, 702, 845, 954, 1032
MaeII ACGT 1 cut(s) 1079
MaeIII GTNAC 4 cut(s) 540, 740, 934, 989
MalI GATC 5 cut(s) 162, 448, 569, 577, 642
MboI GATC 5 cut(s) 160, 446, 567, 575, 640
MflI RGATCY 1 cut(s) 446
MluCI AATT 6 cut(s) 50, 313, 490, 672, 866, 1048
MmeI TCCRAC 3 cut(s) 123, 302, 538
MnlI CCTC 8 cut(s) 75, 163, 287, 321, 357, 466, 475, 980
Mph1103I ATGCAT 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 3 cut(s) 1009, 1020, 1053
MspR9I CCNGG 2 cut(s) 199, 1054
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 4 cut(s) 69, 71, 220, 334
MwoI GCNNNNNNNGC 4 cut(s) 351, 825, 894, 1005
NciI CCSGG 1 cut(s) 1054
NdeI CATATG 1 cut(s) 404
NdeII GATC 5 cut(s) 160, 446, 567, 575, 640
NlaIII CATG 6 cut(s) 237, 309, 625, 656, 713, 1008
NmuCI GTSAC 3 cut(s) 540, 934, 989
NruI TCGCGA 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 2 cut(s) 713, 1008
OliI CACNNNNGTG 1 cut(s) 966
PaeI GCATGC 1 cut(s) 713
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 6 cut(s) 215, 321, 413, 719, 1027, 1154
PkrI GCNGC 3 cut(s) 670, 700, 887
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspPI GGNCC 1 cut(s) 242
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 698
RruI TCGCGA 1 cut(s) 334
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 966
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 3 cut(s) 669, 699, 886
Sau3AI GATC 5 cut(s) 160, 446, 567, 575, 640
Sau96I GGNCC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 199, 1054
SfaNI GCATC 1 cut(s) 722
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 966
SphI GCATGC 1 cut(s) 713
Sse9I AATT 6 cut(s) 50, 313, 490, 672, 866, 1048
SsiI CCGC 6 cut(s) 318, 529, 857, 885, 888, 978
SspMI CTAG 5 cut(s) 444, 702, 845, 954, 1032
StyD4I CCNGG 2 cut(s) 197, 1052
StyI CCWWGG 2 cut(s) 227, 431
TaaI ACNGT 6 cut(s) 251, 755, 806, 836, 905, 1151
TaiI ACGT 1 cut(s) 1082
TaqI TCGA 5 cut(s) 262, 324, 420, 524, 923
TasI AATT 6 cut(s) 50, 313, 490, 672, 866, 1048
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 6 cut(s) 215, 321, 413, 719, 1027, 1154
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TscAI CASTG 3 cut(s) 651, 1045, 1156
TseFI GTSAC 3 cut(s) 540, 934, 989
TseI GCWGC 2 cut(s) 668, 698
Tsp45I GTSAC 3 cut(s) 540, 934, 989
TspDTI ATGAA 8 cut(s) 114, 193, 294, 438, 638, 641, 740, 791
TspRI CASTG 3 cut(s) 651, 1045, 1156
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 3 cut(s) 50, 866, 1048
XceI RCATGY 2 cut(s) 713, 1008
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 5 cut(s) 444, 702, 845, 954, 1032
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.