MD13G1078100.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013263
Physical Location & Seq
Forward (+)
5499548 .. 5502555
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1078100.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGTCGACACATAAGGAGGGGAAGAGGCGCGTGTTGTCTAATTTTCAGCCACACGCGAGACGTCAGCGTAGCAACGCGACGCGACTCTCCCACTGTCAAAGCCGCTTGCAGCCTGCAAGGTCTCGGAGCTTTTGTTTTTCATGTCAAATGTCGTCGCTTTGTTGCTCTGGACTCGCACTGCAATGGAGGCACAGGAGCGTCCGCTTCTTTCTTTTTCTTCGCTCCAACACAAGGACTAGTTGCAGTCGCAGCAGTATTACGTTCGTGCCCAGAGTCACTTCATCTTCAACCAACGACGACGCCGTCTCAGCCCCCAAACCACCGCAGTTGGGTTATGATCCTTCCGAAGACTTATTCGGGCTCGAGGCCAATTCAAAGCCGAGGAATGTTAGTCTGGGTGCGCGCAAACCAAGGTCGTGGTTTGGTCCAAACGGGCAGTATATCAGGGAGCTGCCTTGCCCGAGTTGCCGGGGGAGGGGATATACTCCTTGTTCGGAGTGTGGTATAGAAAGGTCCAGATCAGATTGTTCACAGTGTGTTGGGAAGGGCATAATGACTTGTCGTCAGTGTTCGGGAGAGTGTGTCATATGGGAAGAGTCGATTGATGAACGACCATGGGAGAAAGCCCGCTCAATTTCTCCGCTCAGAGTGAAGGAAGATGATGAAGTTGACAATTTAGACATAAAGCTAGTAAAGAGAAAATCGAAGCGAGTGTACCAATCACCACCACCTGAAGTTGGACTGAAGATCAGCCGGTCTCTAAAAAGTCTGAATGCCAAAACCGGTCTATTTAGTAAGAGAATGAAGATCATCCATCGCGATCCTATGCTTCATGCTCAAAGAGTGGCTGCAATTAAGAAAGCCAAAGGAACTGCTGCAGCAAGAAAGCATACTTCGGAGGCTTTAAAAGCTTTCTTTAGTGACCCAGAAAACCGTCGCAAAAGGAGCATTTCCATGACGGGAGTAAAATTTTACTGCCGAAACTGCGGACGCGAGGGGCATAGAAGACACTACTGTCCAGAATTAAAGGGTGGTTTGATAAATAGACAGTTCACCTGTGGCGTTTGCGGCGAAAAGGGCCATAACAGAAGAACCTGTCCAAAGTCGAGGTTGATGAGCAGTCACAAAAGCGGGGTTAGAAGACATCACCACTGCAAGATATGTGGTCAAAGAGGCCATAATCGCAGGACATGCCCCAAACTTATTGGGGCGAAGGTCAAGCTTGGCAGCAGAAGAACATACAAGTGCAGGATGTGCCAAGAGAATGGGCATAATAGTAGGACATGTCCTAATAGATTTGTATCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_2

Protein Analysis

437

Amino Acids

49.5

Weight (kDa)

10.4

Isoelectric Point (pI)

66.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20220 AT5G20220 AT5G20220 AT5G20220
fragaria_vesca FvH4_4g31851
malus_domestica MD13G1078100.v1.1
prunus_persica Prupe.1G269000_v2.0.a1
pyrus_communis pycom13g06760
rosa_chinensis RchiOBHm_Chr4g0440521
rosa_laevigata RLG00000006172
rosa_roxburghii Rroxscaffold_5G00381350
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378100 Rh4BG390700 Rh4CG405400 Rh4DG384400
rosa_wichuraiana Rw4G032360 Rw4G032460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 302, 1014
AatII GACGTC 1 cut(s) 64
AccBSI CCGCTC 2 cut(s) 630, 643
AccI GTMKAC 1 cut(s) 5
AccII CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
AciI CCGC 8 cut(s) 103, 202, 323, 628, 641, 987, 1068, 1131
AclWI GGATC 2 cut(s) 332, 815
AcsI RAATTY 1 cut(s) 968
AcuI CTGAAG 2 cut(s) 753, 764
AcyI GRCGYC 2 cut(s) 61, 300
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 4 cut(s) 231, 329, 475, 737
AflIII ACRYGT 1 cut(s) 1283
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 2 cut(s) 288, 375
AhdI GACNNNNNGTC 1 cut(s) 561
AhlI ACTAGT 1 cut(s) 236
AluBI AGCT 5 cut(s) 129, 451, 688, 911, 1222
AluI AGCT 5 cut(s) 129, 451, 688, 911, 1222
Alw26I GTCTC 4 cut(s) 52, 126, 310, 762
AlwI GGATC 2 cut(s) 332, 815
Ama87I CYCGRG 2 cut(s) 362, 460
AoxI GGCC 3 cut(s) 366, 1078, 1174
ApeKI GCWGC 7 cut(s) 109, 249, 451, 848, 875, 878, 1227
ApoI RAATTY 1 cut(s) 968
AsiGI ACCGGT 1 cut(s) 782
AspLEI GCGC 3 cut(s) 30, 403, 405
AspS9I GGNCC 3 cut(s) 425, 513, 1078
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 714, 1045, 1139
AvaI CYCGRG 2 cut(s) 362, 460
AvaII GGWCC 2 cut(s) 425, 513
BaeGI GKGCMC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 698, 730
BbsI GAAGAC 3 cut(s) 354, 1012, 1147
BbvI GCAGC 7 cut(s) 121, 261, 438, 835, 862, 890, 1239
BccI CCATC 1 cut(s) 822
BceAI ACGGC 1 cut(s) 287
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 4 cut(s) 52, 126, 310, 762
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 2 cut(s) 237, 689
BfmI CTRYAG 1 cut(s) 876
BisI GCNGC 9 cut(s) 103, 110, 250, 452, 849, 876, 879, 1069, 1228
BlsI GCNGC 9 cut(s) 104, 111, 251, 453, 850, 877, 880, 1070, 1229
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 2 cut(s) 425, 513
BmeRI GACNNNNNGTC 1 cut(s) 561
BmeT110I CYCGRG 2 cut(s) 362, 460
BmgT120I GGNCC 3 cut(s) 425, 513, 1078
BmrFI CCNGG 1 cut(s) 470
BpiI GAAGAC 3 cut(s) 354, 1012, 1147
BpuMI CCSGG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 1300
BsaHI GRCGYC 2 cut(s) 61, 300
BsaI GGTCTC 2 cut(s) 126, 762
BsaJI CCNNGG 4 cut(s) 380, 410, 469, 614
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 4 cut(s) 118, 148, 567, 597
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 329, 475, 737
Bse118I RCCGGY 2 cut(s) 753, 782
Bse3DI GCAATG 1 cut(s) 188
Bse8I GATNNNNATC 1 cut(s) 1300
BseDI CCNNGG 4 cut(s) 380, 410, 469, 614
BseGI GGATG 2 cut(s) 810, 1257
BseJI GATNNNNATC 1 cut(s) 1300
BseLI CCNNNNNNNGG 4 cut(s) 231, 329, 475, 737
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 321, 658
BsePI GCGCGC 1 cut(s) 401
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 7 cut(s) 121, 261, 438, 835, 862, 890, 1239
BsgI GTGCAG 1 cut(s) 1267
Bsh1236I CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
BshFI GGCC 3 cut(s) 368, 1080, 1176
BshTI ACCGGT 1 cut(s) 782
BsiHKCI CYCGRG 2 cut(s) 362, 460
BsiSI CCGG 3 cut(s) 469, 754, 783
BslI CCNNNNNNNGG 4 cut(s) 231, 329, 475, 737
BsmAI GTCTC 4 cut(s) 52, 126, 310, 762
BsmBI CGTCTC 2 cut(s) 52, 310
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 3 cut(s) 368, 1080, 1176
Bso31I GGTCTC 2 cut(s) 126, 762
BsoBI CYCGRG 2 cut(s) 362, 460
Bsp1286I GDGCHC 2 cut(s) 270, 363
Bsp143I GATC 5 cut(s) 337, 518, 747, 807, 820
Bsp19I CCATGG 1 cut(s) 614
Bsp68I TCGCGA 1 cut(s) 819
BspACI CCGC 8 cut(s) 103, 202, 323, 628, 641, 987, 1068, 1131
BspANI GGCC 3 cut(s) 368, 1080, 1176
BspCNI CTCAG 2 cut(s) 320, 657
BspFNI CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 2 cut(s) 332, 815
BspTNI GGTCTC 2 cut(s) 126, 762
BsrBI CCGCTC 2 cut(s) 630, 643
BsrDI GCAATG 1 cut(s) 188
BsrFI RCCGGY 2 cut(s) 753, 782
BssAI RCCGGY 2 cut(s) 753, 782
BssECI CCNNGG 4 cut(s) 380, 410, 469, 614
BssHII GCGCGC 1 cut(s) 401
BssMI GATC 5 cut(s) 337, 518, 747, 807, 820
BssNI GRCGYC 2 cut(s) 61, 300
BssT1I CCWWGG 2 cut(s) 410, 614
Bst4CI ACNGT 5 cut(s) 95, 534, 935, 1016, 1050
Bst6I CTCTTC 2 cut(s) 17, 588
BstACI GRCGYC 2 cut(s) 61, 300
BstAPI GCANNNNNTGC 2 cut(s) 1191, 1254
BstC8I GCNNGC 4 cut(s) 107, 114, 403, 628
BstDEI CTNAG 2 cut(s) 307, 644
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 2 cut(s) 810, 1257
BstFNI CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
BstHHI GCGC 3 cut(s) 30, 403, 405
BstKTI GATC 5 cut(s) 340, 521, 750, 810, 823
BstMAI GTCTC 4 cut(s) 52, 126, 310, 762
BstMBI GATC 5 cut(s) 337, 518, 747, 807, 820
BstNSI RCATGY 2 cut(s) 1194, 1287
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 1 cut(s) 270
BstUI CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
BstV1I GCAGC 7 cut(s) 121, 261, 438, 835, 862, 890, 1239
BstV2I GAAGAC 3 cut(s) 354, 1012, 1147
BstXI CCANNNNNNTGG 2 cut(s) 417, 1265
BsuRI GGCC 3 cut(s) 368, 1080, 1176
BtgI CCRYGG 1 cut(s) 614
BtgZI GCGATG 1 cut(s) 800
BtsCI GGATG 2 cut(s) 810, 1257
BtsI GCAGTG 2 cut(s) 176, 1150
BtsIMutI CAGTG 5 cut(s) 91, 176, 539, 572, 1150
BtuMI TCGCGA 1 cut(s) 819
Cac8I GCNNGC 4 cut(s) 107, 114, 403, 628
CfoI GCGC 3 cut(s) 30, 403, 405
Cfr10I RCCGGY 2 cut(s) 753, 782
Cfr13I GGNCC 3 cut(s) 425, 513, 1078
CseI GACGC 4 cut(s) 88, 187, 308, 999
Csp6I GTAC 1 cut(s) 715
CspAI ACCGGT 1 cut(s) 782
CspCI CAANNNNNGTGG 4 cut(s) 309, 344, 1144, 1179
CviAII CATG 6 cut(s) 141, 615, 833, 955, 1191, 1284
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 2 cut(s) 307, 644
DpnI GATC 5 cut(s) 339, 520, 749, 809, 822
DpnII GATC 5 cut(s) 337, 518, 747, 807, 820
DraI TTTAAA 1 cut(s) 906
DraIII CACNNNGTG 1 cut(s) 536
DrdI GACNNNNNNGTC 2 cut(s) 302, 1014
DriI GACNNNNNGTC 1 cut(s) 561
DseDI GACNNNNNNGTC 2 cut(s) 302, 1014
Eam1104I CTCTTC 2 cut(s) 17, 588
Eam1105I GACNNNNNGTC 1 cut(s) 561
EarI CTCTTC 2 cut(s) 17, 588
Eco130I CCWWGG 2 cut(s) 410, 614
Eco24I GRGCYC 1 cut(s) 363
Eco31I GGTCTC 2 cut(s) 126, 762
Eco47I GGWCC 2 cut(s) 425, 513
Eco57I CTGAAG 2 cut(s) 753, 764
Eco88I CYCGRG 2 cut(s) 362, 460
EcoT14I CCWWGG 2 cut(s) 410, 614
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 410, 614
Esp3I CGTCTC 2 cut(s) 52, 310
FaeI CATG 6 cut(s) 144, 618, 836, 958, 1194, 1287
FatI CATG 6 cut(s) 140, 614, 832, 954, 1190, 1283
FauI CCCGC 2 cut(s) 635, 1124
FauNDI CATATG 1 cut(s) 587
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 9 cut(s) 103, 110, 250, 452, 849, 876, 879, 1069, 1228
FokI GGATG 2 cut(s) 797, 1264
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 9 cut(s) 103, 110, 250, 452, 849, 876, 879, 1069, 1228
FspBI CTAG 2 cut(s) 237, 689
GlaI GCGC 3 cut(s) 29, 402, 404
GluI GCNGC 9 cut(s) 103, 110, 250, 452, 849, 876, 879, 1069, 1228
HaeIII GGCC 3 cut(s) 368, 1080, 1176
HapII CCGG 3 cut(s) 469, 754, 783
HgaI GACGC 4 cut(s) 88, 187, 308, 999
HhaI GCGC 3 cut(s) 30, 403, 405
Hin1I GRCGYC 2 cut(s) 61, 300
Hin1II CATG 6 cut(s) 144, 618, 836, 958, 1194, 1287
Hin6I GCGC 3 cut(s) 28, 401, 403
HinP1I GCGC 3 cut(s) 28, 401, 403
HincII GTYRAC 2 cut(s) 6, 670
HindII GTYRAC 2 cut(s) 6, 670
HindIII AAGCTT 2 cut(s) 909, 1220
HinfI GANTC 4 cut(s) 84, 171, 273, 596
HpaII CCGG 3 cut(s) 469, 754, 783
HphI GGTGA 3 cut(s) 714, 1045, 1139
Hpy166II GTNNAC 5 cut(s) 6, 530, 670, 715, 1053
Hpy188I TCNGA 7 cut(s) 126, 346, 496, 523, 647, 771, 898
Hpy188III TCNNGA 6 cut(s) 168, 516, 573, 818, 1019, 1308
Hpy8I GTNNAC 5 cut(s) 6, 530, 670, 715, 1053
Hpy99I CGWCG 5 cut(s) 82, 157, 299, 302, 939
HpyAV CCTTC 4 cut(s) 351, 538, 646, 1207
HpyCH4III ACNGT 5 cut(s) 95, 534, 935, 1016, 1050
HpyCH4IV ACGT 2 cut(s) 61, 260
HpyCH4V TGCA 8 cut(s) 109, 116, 181, 243, 851, 878, 1155, 1248
HpyF3I CTNAG 2 cut(s) 307, 644
HpySE526I ACGT 2 cut(s) 61, 260
Hsp92I GRCGYC 2 cut(s) 61, 300
Hsp92II CATG 6 cut(s) 144, 618, 836, 958, 1194, 1287
HspAI GCGC 3 cut(s) 28, 401, 403
Kzo9I GATC 5 cut(s) 337, 518, 747, 807, 820
LmnI GCTCC 5 cut(s) 126, 195, 227, 448, 945
Lsp1109I GCAGC 7 cut(s) 121, 261, 438, 835, 862, 890, 1239
MaeI CTAG 2 cut(s) 237, 689
MaeII ACGT 2 cut(s) 61, 260
MaeIII GTNAC 3 cut(s) 274, 920, 1121
MalI GATC 5 cut(s) 339, 520, 749, 809, 822
MbiI CCGCTC 2 cut(s) 630, 643
MboI GATC 5 cut(s) 337, 518, 747, 807, 820
MhlI GDGCHC 2 cut(s) 270, 363
MluCI AATT 7 cut(s) 40, 370, 633, 673, 852, 968, 1022
MlyI GAGTC 4 cut(s) 78, 165, 282, 605
MmeI TCCRAC 2 cut(s) 249, 718
MseI TTAA 3 cut(s) 855, 905, 1025
MslI CAYNNNNRTG 3 cut(s) 181, 953, 1243
MspI CCGG 3 cut(s) 469, 754, 783
MspR9I CCNGG 1 cut(s) 470
Mva1269I GAATGC 1 cut(s) 778
MvnI CGCG 7 cut(s) 30, 56, 77, 82, 403, 819, 993
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 1 cut(s) 587
NdeII GATC 5 cut(s) 337, 518, 747, 807, 820
NlaIII CATG 6 cut(s) 144, 618, 836, 958, 1194, 1287
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 3 cut(s) 274, 920, 1121
NruI TCGCGA 1 cut(s) 819
NspI RCATGY 2 cut(s) 1194, 1287
PaeR7I CTCGAG 1 cut(s) 362
PauI GCGCGC 1 cut(s) 401
PciI ACATGT 1 cut(s) 1283
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 778
PflFI GACNNNGTC 1 cut(s) 302
PinAI ACCGGT 1 cut(s) 782
PkrI GCNGC 9 cut(s) 104, 111, 251, 453, 850, 877, 880, 1070, 1229
PleI GAGTC 4 cut(s) 78, 165, 281, 604
PpsI GAGTC 4 cut(s) 78, 165, 281, 604
PscI ACATGT 1 cut(s) 1283
PspPI GGNCC 3 cut(s) 425, 513, 1078
PspXI VCTCGAGB 1 cut(s) 362
PstI CTGCAG 1 cut(s) 880
PsyI GACNNNGTC 1 cut(s) 302
PteI GCGCGC 1 cut(s) 401
RruI TCGCGA 1 cut(s) 819
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 3 cut(s) 181, 953, 1243
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 855, 905, 1025
SatI GCNGC 9 cut(s) 103, 110, 250, 452, 849, 876, 879, 1069, 1228
Sau3AI GATC 5 cut(s) 337, 518, 747, 807, 820
Sau96I GGNCC 3 cut(s) 425, 513, 1078
SchI GAGTC 4 cut(s) 78, 165, 282, 605
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 2 cut(s) 270, 363
SfcI CTRYAG 1 cut(s) 876
Sfr274I CTCGAG 1 cut(s) 362
SinI GGWCC 2 cut(s) 425, 513
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 3 cut(s) 181, 953, 1243
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SpeI ACTAGT 1 cut(s) 236
Sse9I AATT 7 cut(s) 40, 370, 633, 673, 852, 968, 1022
SsiI CCGC 8 cut(s) 103, 202, 323, 628, 641, 987, 1068, 1131
SspMI CTAG 2 cut(s) 237, 689
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 2 cut(s) 410, 614
TaaI ACNGT 5 cut(s) 95, 534, 935, 1016, 1050
TaiI ACGT 2 cut(s) 64, 263
TaqI TCGA 5 cut(s) 5, 363, 599, 704, 1106
TasI AATT 7 cut(s) 40, 370, 633, 673, 852, 968, 1022
TauI GCSGC 2 cut(s) 105, 1071
Tru1I TTAA 3 cut(s) 855, 905, 1025
Tru9I TTAA 3 cut(s) 855, 905, 1025
TscAI CASTG 5 cut(s) 98, 183, 539, 572, 1157
TseFI GTSAC 3 cut(s) 274, 920, 1121
TseI GCWGC 7 cut(s) 109, 249, 451, 848, 875, 878, 1227
Tsp45I GTSAC 3 cut(s) 274, 920, 1121
TspDTI ATGAA 6 cut(s) 129, 270, 621, 678, 818, 821
TspRI CASTG 5 cut(s) 98, 183, 539, 572, 1157
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 425, 513
XapI RAATTY 1 cut(s) 968
XceI RCATGY 2 cut(s) 1194, 1287
XhoI CTCGAG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 237, 689
ZraI GACGTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.