pycom13g06760

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013263
Physical Location & Seq
Forward (+)
4491636 .. 4493692
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06760.1

Sequence Viewer

Length: 801 bp
ATGCCATGTCGACACATAAGGAGGGGAAAAGGCGCGTGTTGTCTAATTTTCAGCCACACGCGAGACGTCAGTGTAGCAACGCGACGCGACTCTTCCACTCTCAAAGCCGCTTGCAGCCTGCGAAGTCTCGAGCGCATTTTTTTTTTTTTTTTTTCATGTCAAATGTCGTCGCTTTGTTGCTCTGGACTCGCACTGCAATGGAGGCACAAGAGCGTCCGCTTCTTTCTTTTTCTTCGCTCCAACACAAGGACCAGTTGCAGTCGCAGCAGTATTACGTTCGTGCCCAGAGTTACTTCATCTTCAACCAACGACGACGCCGTCTCAGCCCCCAAGCCACCGCAGCTGGGTTATGATCCTTCCGAAGACTTATTCGGGCTCGAGGCCAATTCAAAGCCGAGGACTGTTAGTTTGGGTGCGCGCAAACCAAGGTCGTGGTTTGGTCCAAATGGGCAGTATATCAGGGAGCTGCCTTGCCCGAGTTGCCGGGGGAGGGGATATACTCCTTGTTCAGAGTGCGGTATAGAAAGGTCCAGATCAGATTGTTCACAGTGTGTTGGGAAGGGCATAATGACTTGTCGTCAGTGTTCAGGAGACTGTGTCATATGGGAAGAGTCGATTGATGAACGACCATGGGAGAATGCTCGCTCAGTTTCTCCGCTCAGAGTGAAGGAAGATGATGAAGTTGACAATTTAGACATAAAGCTAGTGAAGAGAAAATCGAAGCGAGTGTACCAATCACCACCACCTGAAGTTGGACTGAAGATCAGCCGGTCTCTAAAATTGGATGATTGCAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_2

Protein Analysis

267

Amino Acids

29.95

Weight (kDa)

9.3

Isoelectric Point (pI)

64.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20220 AT5G20220 AT5G20220 AT5G20220
fragaria_vesca FvH4_4g31851
malus_domestica MD13G1078100.v1.1
prunus_persica Prupe.1G269000_v2.0.a1
pyrus_communis pycom13g06760
rosa_chinensis RchiOBHm_Chr4g0440521
rosa_laevigata RLG00000006172
rosa_roxburghii Rroxscaffold_5G00381350
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378100 Rh4BG390700 Rh4CG405400 Rh4DG384400
rosa_wichuraiana Rw4G032360 Rw4G032460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 10
AccII CGCG 5 cut(s) 35, 61, 82, 87, 418
AciI CCGC 5 cut(s) 108, 217, 338, 516, 656
AclWI GGATC 1 cut(s) 347
AcuI CTGAAG 2 cut(s) 768, 779
AcyI GRCGYC 2 cut(s) 66, 315
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 4 cut(s) 246, 344, 490, 752
AgsI TTSAA 2 cut(s) 303, 390
AhdI GACNNNNNGTC 1 cut(s) 576
AluBI AGCT 3 cut(s) 343, 466, 703
AluI AGCT 3 cut(s) 343, 466, 703
Alw26I GTCTC 5 cut(s) 57, 131, 325, 585, 777
AlwI GGATC 1 cut(s) 347
AlwNI CAGNNNCTG 1 cut(s) 798
Ama87I CYCGRG 3 cut(s) 128, 377, 475
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 4 cut(s) 114, 264, 340, 466
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AspLEI GCGC 4 cut(s) 35, 135, 418, 420
AspS9I GGNCC 3 cut(s) 249, 440, 528
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 729
AvaI CYCGRG 3 cut(s) 128, 377, 475
AvaII GGWCC 3 cut(s) 249, 440, 528
BaeGI GKGCMC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 378
BarI GAAGNNNNNNTAC 2 cut(s) 713, 745
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 4 cut(s) 126, 276, 352, 453
BceAI ACGGC 1 cut(s) 302
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 5 cut(s) 57, 131, 325, 585, 777
BfaI CTAG 1 cut(s) 704
BisI GCNGC 5 cut(s) 108, 115, 265, 341, 467
BlsI GCNGC 5 cut(s) 109, 116, 266, 342, 468
Bme1390I CCNGG 1 cut(s) 485
Bme18I GGWCC 3 cut(s) 249, 440, 528
BmeRI GACNNNNNGTC 1 cut(s) 576
BmeT110I CYCGRG 3 cut(s) 128, 377, 475
BmgT120I GGNCC 3 cut(s) 249, 440, 528
BmrFI CCNGG 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 485
BsaHI GRCGYC 2 cut(s) 66, 315
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 4 cut(s) 395, 425, 484, 629
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 344, 490, 752
Bse118I RCCGGY 1 cut(s) 768
Bse1I ACTGG 1 cut(s) 252
Bse3DI GCAATG 1 cut(s) 203
BseDI CCNNGG 4 cut(s) 395, 425, 484, 629
BseGI GGATG 1 cut(s) 790
BseLI CCNNNNNNNGG 4 cut(s) 246, 344, 490, 752
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 3 cut(s) 336, 660, 673
BseNI ACTGG 1 cut(s) 252
BsePI GCGCGC 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 4 cut(s) 126, 276, 352, 453
BseYI CCCAGC 1 cut(s) 343
Bsh1236I CGCG 5 cut(s) 35, 61, 82, 87, 418
BshFI GGCC 1 cut(s) 383
BsiHKCI CYCGRG 3 cut(s) 128, 377, 475
BsiSI CCGG 2 cut(s) 484, 769
BslI CCNNNNNNNGG 4 cut(s) 246, 344, 490, 752
BsmAI GTCTC 5 cut(s) 57, 131, 325, 585, 777
BsmBI CGTCTC 2 cut(s) 57, 325
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 3 cut(s) 128, 377, 475
Bsp1286I GDGCHC 2 cut(s) 285, 378
Bsp143I GATC 3 cut(s) 352, 533, 762
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 5 cut(s) 108, 217, 338, 516, 656
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 3 cut(s) 335, 659, 672
BspFNI CGCG 5 cut(s) 35, 61, 82, 87, 418
BspPI GGATC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 777
BsrBI CCGCTC 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 768
BsrI ACTGG 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 768
BssECI CCNNGG 4 cut(s) 395, 425, 484, 629
BssHII GCGCGC 1 cut(s) 416
BssMI GATC 3 cut(s) 352, 533, 762
BssNI GRCGYC 2 cut(s) 66, 315
BssT1I CCWWGG 2 cut(s) 425, 629
Bst4CI ACNGT 3 cut(s) 403, 549, 596
Bst6I CTCTTC 3 cut(s) 97, 603, 704
BstACI GRCGYC 2 cut(s) 66, 315
BstC8I GCNNGC 4 cut(s) 112, 119, 418, 643
BstDEI CTNAG 3 cut(s) 322, 646, 659
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 1 cut(s) 790
BstFNI CGCG 5 cut(s) 35, 61, 82, 87, 418
BstHHI GCGC 4 cut(s) 35, 135, 418, 420
BstKTI GATC 3 cut(s) 355, 536, 765
BstMAI GTCTC 5 cut(s) 57, 131, 325, 585, 777
BstMBI GATC 3 cut(s) 352, 533, 762
BstMWI GCNNNNNNNGC 5 cut(s) 202, 264, 323, 340, 480
BstSCI CCNGG 1 cut(s) 483
BstSLI GKGCMC 1 cut(s) 285
BstUI CGCG 5 cut(s) 35, 61, 82, 87, 418
BstV1I GCAGC 4 cut(s) 126, 276, 352, 453
BstV2I GAAGAC 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 432
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 1 cut(s) 790
BtsI GCAGTG 1 cut(s) 191
BtsIMutI CAGTG 4 cut(s) 76, 191, 554, 587
Cac8I GCNNGC 4 cut(s) 112, 119, 418, 643
CaiI CAGNNNCTG 1 cut(s) 798
CfoI GCGC 4 cut(s) 35, 135, 418, 420
Cfr10I RCCGGY 1 cut(s) 768
Cfr13I GGNCC 3 cut(s) 249, 440, 528
CseI GACGC 3 cut(s) 93, 202, 323
Csp6I GTAC 1 cut(s) 730
CviAII CATG 3 cut(s) 6, 156, 630
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 3 cut(s) 322, 646, 659
DpnI GATC 3 cut(s) 354, 535, 764
DpnII GATC 3 cut(s) 352, 533, 762
DraIII CACNNNGTG 1 cut(s) 551
DrdI GACNNNNNNGTC 1 cut(s) 317
DriI GACNNNNNGTC 1 cut(s) 576
DseDI GACNNNNNNGTC 1 cut(s) 317
Eam1104I CTCTTC 3 cut(s) 97, 603, 704
Eam1105I GACNNNNNGTC 1 cut(s) 576
EarI CTCTTC 3 cut(s) 97, 603, 704
Eco130I CCWWGG 2 cut(s) 425, 629
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 3 cut(s) 249, 440, 528
Eco57I CTGAAG 2 cut(s) 768, 779
Eco88I CYCGRG 3 cut(s) 128, 377, 475
EcoT14I CCWWGG 2 cut(s) 425, 629
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 425, 629
Esp3I CGTCTC 2 cut(s) 57, 325
FaeI CATG 3 cut(s) 9, 159, 633
FatI CATG 3 cut(s) 5, 155, 629
FauNDI CATATG 1 cut(s) 602
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 5 cut(s) 108, 115, 265, 341, 467
FokI GGATG 1 cut(s) 797
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 5 cut(s) 108, 115, 265, 341, 467
FspBI CTAG 1 cut(s) 704
GlaI GCGC 4 cut(s) 34, 134, 417, 419
GluI GCNGC 5 cut(s) 108, 115, 265, 341, 467
GsaI CCCAGC 1 cut(s) 347
HaeIII GGCC 1 cut(s) 383
HapII CCGG 2 cut(s) 484, 769
HgaI GACGC 3 cut(s) 93, 202, 323
HhaI GCGC 4 cut(s) 35, 135, 418, 420
Hin1I GRCGYC 2 cut(s) 66, 315
Hin1II CATG 3 cut(s) 9, 159, 633
Hin6I GCGC 4 cut(s) 33, 133, 416, 418
HinP1I GCGC 4 cut(s) 33, 133, 416, 418
HincII GTYRAC 2 cut(s) 11, 685
HindII GTYRAC 2 cut(s) 11, 685
HinfI GANTC 4 cut(s) 89, 186, 611, 795
HpaII CCGG 2 cut(s) 484, 769
HphI GGTGA 1 cut(s) 729
Hpy166II GTNNAC 4 cut(s) 11, 545, 685, 730
Hpy188I TCNGA 5 cut(s) 361, 511, 538, 662, 800
Hpy188III TCNNGA 4 cut(s) 128, 183, 531, 588
Hpy8I GTNNAC 4 cut(s) 11, 545, 685, 730
Hpy99I CGWCG 4 cut(s) 87, 172, 314, 317
HpyAV CCTTC 3 cut(s) 366, 553, 661
HpyCH4III ACNGT 3 cut(s) 403, 549, 596
HpyCH4IV ACGT 2 cut(s) 66, 275
HpyCH4V TGCA 4 cut(s) 114, 196, 258, 792
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 264, 323, 340, 480
HpyF3I CTNAG 3 cut(s) 322, 646, 659
HpySE526I ACGT 2 cut(s) 66, 275
Hsp92I GRCGYC 2 cut(s) 66, 315
Hsp92II CATG 3 cut(s) 9, 159, 633
HspAI GCGC 4 cut(s) 33, 133, 416, 418
Kzo9I GATC 3 cut(s) 352, 533, 762
LmnI GCTCC 2 cut(s) 242, 463
Lsp1109I GCAGC 4 cut(s) 126, 276, 352, 453
MaeI CTAG 1 cut(s) 704
MaeII ACGT 2 cut(s) 66, 275
MaeIII GTNAC 1 cut(s) 289
MalI GATC 3 cut(s) 354, 535, 764
MbiI CCGCTC 1 cut(s) 658
MboI GATC 3 cut(s) 352, 533, 762
MboII GAAGA 8 cut(s) 84, 224, 291, 374, 620, 683, 721, 772
MhlI GDGCHC 2 cut(s) 285, 378
MluCI AATT 4 cut(s) 45, 385, 688, 779
MlyI GAGTC 3 cut(s) 83, 180, 620
MmeI TCCRAC 2 cut(s) 264, 733
MnlI CCTC 5 cut(s) 15, 195, 373, 390, 483
MslI CAYNNNNRTG 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 2 cut(s) 484, 769
MspR9I CCNGG 1 cut(s) 485
Mva1269I GAATGC 1 cut(s) 643
MvnI CGCG 5 cut(s) 35, 61, 82, 87, 418
MwoI GCNNNNNNNGC 5 cut(s) 202, 264, 323, 340, 480
NciI CCSGG 1 cut(s) 485
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 602
NdeII GATC 3 cut(s) 352, 533, 762
NlaIII CATG 3 cut(s) 9, 159, 633
NmeAIII GCCGAG 1 cut(s) 420
PaeR7I CTCGAG 2 cut(s) 128, 377
PauI GCGCGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 643
PflFI GACNNNGTC 2 cut(s) 317, 596
PkrI GCNGC 5 cut(s) 109, 116, 266, 342, 468
PleI GAGTC 3 cut(s) 83, 180, 619
PpsI GAGTC 3 cut(s) 83, 180, 619
PspFI CCCAGC 1 cut(s) 343
PspPI GGNCC 3 cut(s) 249, 440, 528
PspXI VCTCGAGB 1 cut(s) 377
PstNI CAGNNNCTG 1 cut(s) 798
PsyI GACNNNGTC 2 cut(s) 317, 596
PteI GCGCGC 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
RseI CAYNNNNRTG 1 cut(s) 196
SalI GTCGAC 1 cut(s) 9
SatI GCNGC 5 cut(s) 108, 115, 265, 341, 467
Sau3AI GATC 3 cut(s) 352, 533, 762
Sau96I GGNCC 3 cut(s) 249, 440, 528
SchI GAGTC 3 cut(s) 83, 180, 620
ScrFI CCNGG 1 cut(s) 485
SduI GDGCHC 2 cut(s) 285, 378
SetI ASST 8 cut(s) 69, 278, 345, 431, 468, 530, 705, 748
Sfr274I CTCGAG 2 cut(s) 128, 377
SinI GGWCC 3 cut(s) 249, 440, 528
SlaI CTCGAG 2 cut(s) 128, 377
SmiMI CAYNNNNRTG 1 cut(s) 196
SmlI CTYRAG 2 cut(s) 128, 377
SmoI CTYRAG 2 cut(s) 128, 377
Sse9I AATT 4 cut(s) 45, 385, 688, 779
SsiI CCGC 5 cut(s) 108, 217, 338, 516, 656
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 1 cut(s) 483
StyI CCWWGG 2 cut(s) 425, 629
TaaI ACNGT 3 cut(s) 403, 549, 596
TaiI ACGT 2 cut(s) 69, 278
TaqI TCGA 5 cut(s) 10, 129, 378, 614, 719
TasI AATT 4 cut(s) 45, 385, 688, 779
TauI GCSGC 1 cut(s) 110
TscAI CASTG 4 cut(s) 76, 198, 554, 587
TseI GCWGC 4 cut(s) 114, 264, 340, 466
TspDTI ATGAA 4 cut(s) 144, 285, 636, 693
TspRI CASTG 4 cut(s) 76, 198, 554, 587
Tth111I GACNNNGTC 2 cut(s) 317, 596
VpaK11BI GGWCC 3 cut(s) 249, 440, 528
XhoI CTCGAG 2 cut(s) 128, 377
XmiI GTMKAC 1 cut(s) 10
XspI CTAG 1 cut(s) 704
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.