Rw4G032460

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013263
Physical Location & Seq
Forward (+)
59584970 .. 59587921
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032460.1

Sequence Viewer

Length: 1215 bp
ATGTCGTCGTTGTGTTCGTTAGGGCTTGCGCCGCAGTTGAGTCATAAGAGTGCGAGCTTCTTGTTCTTATTCAAATTCAAGAGAAGGACTTTAACCTTCGTCCCCAGAGTCACTTCTTCTTCTTCTTCTTCAAACGGCGACGACTCTGTTTCGGCGCCGAAACCAAAGCAGTTGGGCTATGATCTTTCTGAGGACCTATTCGGGCTTGAGGGTAATCTGAAGCCAAGGAATGCTAGTTTGGGTGCGCGAAAACCAAGCTCTTGGTTCGGTCCAAATGGTCAGTATATCAGAGAGCTACCATGCCCAAGTTGCCGAGGGAGGGGATATACTCCTTGTACTGAGTGTGGTATAGAAAGGTCCAGATCAGACTGTTCACAGTGCCTTGGCAAGGGCTTGATGACTTGTCGTCAGTGCATGGGAGATTGTGTCATATGGGAAGAGTCGATTGATGAACGGCCATGGGAAAAAGCTCGCTCAATTTCTCCACTTAAAGTGAAGGAAGATGATGAAGTTGACAATTTGGAAATAAAACTGGATGTTAAGAGAAAGTCAAAGCGAGTATACCAGTCACCACCTCCTGAAGTTGGACTGAAGATTAGCCGCTCTCTGAAAAGTCTGAATGCCAAAACTGGTCTATTTAGTAAGAGAATGAAGATTATCCACCGTGATCCTAGGCTTCATGCTCAAAGAGTGGCAGCAATTAAGAAAGCCAAGGGAACTGCCGCTGCTAGAAAGCATACCTCTGAAACTTTAAAAGCTTTCTTTAGTGACCCAGAAAACCGTCGCAGAAGGAGCATTTCCATGCAGGGCATAAAATTTTACTGCAGAAACTGTGGGCGGGAGGGGCATAGGAGACACTACTGTCCAGAACTGAAGGCTAGTTTGATGAATAGGCAGTTCATCTGTGGCGTGTGTGGAGAAAAGGGTCATAACAGAAGAACCTGTCCAACGTTGAGCCGTCCCAAAAGCACTGTTAGAAGACCTCACCATTGCAGAATTTGTGATCAAAGAGGCCACAATCGAACAACATGCCCCAAAGTGATTGGACCAAAGTTTATTGATGTAAAGAGCGGGTTTCACAGTTCTGTAAGCCGAAGAACATACAAGTGCAAGTTGTGCAAAGAAAATGGGCATAATAGTAGGACATGTCCTACTCATAACAGAAATGTACATCCCTCAAAGATTGGTCATCAATCTGAGGAAGGAAATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_2

Protein Analysis

404

Amino Acids

45.62

Weight (kDa)

9.97

Isoelectric Point (pI)

58.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20220 AT5G20220 AT5G20220 AT5G20220
fragaria_vesca FvH4_4g31851
malus_domestica MD13G1078100.v1.1
prunus_persica Prupe.1G269000_v2.0.a1
pyrus_communis pycom13g06760
rosa_chinensis RchiOBHm_Chr4g0440521
rosa_laevigata RLG00000006172
rosa_roxburghii Rroxscaffold_5G00381350
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378100 Rh4BG390700 Rh4CG405400 Rh4DG384400
rosa_wichuraiana Rw4G032360 Rw4G032460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 861
AccB1I GGYRCC 1 cut(s) 154
AccBSI CCGCTC 2 cut(s) 603, 1071
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 247
AciI CCGC 5 cut(s) 32, 601, 723, 838, 1071
AclI AACGTT 1 cut(s) 950
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 3 cut(s) 74, 815, 996
AcuI CTGAAG 4 cut(s) 239, 600, 611, 893
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 337, 1170
AfiI CCNNNNNNNGG 3 cut(s) 319, 388, 584
AflIII ACRYGT 1 cut(s) 1145
AgsI TTSAA 3 cut(s) 73, 79, 132
AhdI GACNNNNNGTC 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 5 cut(s) 57, 258, 295, 470, 758
AluI AGCT 5 cut(s) 57, 258, 295, 470, 758
Alw26I GTCTC 1 cut(s) 847
AlwI GGATC 1 cut(s) 662
AlwNI CAGNNNCTG 1 cut(s) 831
AoxI GGCC 2 cut(s) 455, 1012
ApeKI GCWGC 2 cut(s) 695, 725
ApoI RAATTY 3 cut(s) 74, 815, 996
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 3 cut(s) 31, 157, 247
AspS9I GGNCC 4 cut(s) 193, 269, 357, 1046
AsuHPI GGTGA 2 cut(s) 561, 977
AvaII GGWCC 4 cut(s) 193, 269, 357, 1046
AvrII CCTAGG 1 cut(s) 671
BanI GGYRCC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 985
BbvI GCAGC 2 cut(s) 707, 712
BceAI ACGGC 3 cut(s) 151, 470, 942
BcgI CGANNNNNNTGC 2 cut(s) 1011, 1045
BclI TGATCA 1 cut(s) 1003
BcoDI GTCTC 1 cut(s) 847
BfaI CTAG 4 cut(s) 234, 672, 729, 879
BfmI CTRYAG 1 cut(s) 823
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 5 cut(s) 32, 601, 696, 723, 726
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 5 cut(s) 33, 602, 697, 724, 727
Bme18I GGWCC 4 cut(s) 193, 269, 357, 1046
BmeRI GACNNNNNGTC 1 cut(s) 405
BmgT120I GGNCC 4 cut(s) 193, 269, 357, 1046
BmiI GGNNCC 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 985
BpuEI CTTGAG 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 6 cut(s) 224, 313, 382, 458, 671, 711
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 388, 584
Bse1I ACTGG 3 cut(s) 537, 565, 634
Bse3DI GCAATG 1 cut(s) 988
BseDI CCNNGG 6 cut(s) 224, 313, 382, 458, 671, 711
BseGI GGATG 2 cut(s) 541, 1171
BseLI CCNNNNNNNGG 3 cut(s) 319, 388, 584
BseMI GCAATG 1 cut(s) 988
BseMII CTCAG 3 cut(s) 180, 330, 1188
BseNI ACTGG 3 cut(s) 537, 565, 634
BseXI GCAGC 2 cut(s) 707, 712
Bsh1236I CGCG 1 cut(s) 247
BshFI GGCC 2 cut(s) 457, 1014
BshNI GGYRCC 1 cut(s) 154
BslFI GGGAC 2 cut(s) 86, 945
BslI CCNNNNNNNGG 3 cut(s) 319, 388, 584
BsmAI GTCTC 1 cut(s) 847
BsmFI GGGAC 2 cut(s) 86, 945
BsmI GAATGC 2 cut(s) 235, 625
BsnI GGCC 2 cut(s) 457, 1014
Bsp1407I TGTACA 1 cut(s) 1168
Bsp143I GATC 4 cut(s) 181, 362, 667, 1003
Bsp19I CCATGG 1 cut(s) 458
BspACI CCGC 5 cut(s) 32, 601, 723, 838, 1071
BspANI GGCC 2 cut(s) 457, 1014
BspCNI CTCAG 3 cut(s) 181, 331, 1189
BspFNI CGCG 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 156
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 154
BsrBI CCGCTC 2 cut(s) 603, 1071
BsrDI GCAATG 1 cut(s) 988
BsrGI TGTACA 1 cut(s) 1168
BsrI ACTGG 3 cut(s) 537, 565, 634
BssECI CCNNGG 6 cut(s) 224, 313, 382, 458, 671, 711
BssMI GATC 4 cut(s) 181, 362, 667, 1003
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 5 cut(s) 224, 382, 458, 671, 711
Bst1107I GTATAC 1 cut(s) 562
Bst4CI ACNGT 8 cut(s) 371, 378, 665, 782, 833, 863, 973, 1082
Bst6I CTCTTC 1 cut(s) 432
BstACI GRCGYC 1 cut(s) 155
BstAPI GCANNNNNTGC 1 cut(s) 1116
BstAUI TGTACA 1 cut(s) 1168
BstC8I GCNNGC 3 cut(s) 27, 55, 472
BstDEI CTNAG 3 cut(s) 189, 339, 1197
BstDSI CCRYGG 1 cut(s) 458
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 2 cut(s) 541, 1171
BstFNI CGCG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 3 cut(s) 31, 157, 247
BstKTI GATC 4 cut(s) 184, 365, 670, 1006
BstMAI GTCTC 1 cut(s) 847
BstMBI GATC 4 cut(s) 181, 362, 667, 1003
BstMWI GCNNNNNNNGC 5 cut(s) 31, 309, 792, 844, 1116
BstNSI RCATGY 2 cut(s) 1032, 1149
BstSFI CTRYAG 1 cut(s) 823
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 2 cut(s) 707, 712
BstV2I GAAGAC 1 cut(s) 985
BstXI CCANNNNNNTGG 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 562
BsuRI GGCC 2 cut(s) 457, 1014
BtgI CCRYGG 1 cut(s) 458
BtsCI GGATG 2 cut(s) 541, 1171
BtsIMutI CAGTG 3 cut(s) 383, 416, 969
Cac8I GCNNGC 3 cut(s) 27, 55, 472
CaiI CAGNNNCTG 1 cut(s) 831
CfoI GCGC 3 cut(s) 31, 157, 247
Cfr13I GGNCC 4 cut(s) 193, 269, 357, 1046
Csp6I GTAC 2 cut(s) 336, 1169
CviAII CATG 7 cut(s) 300, 415, 459, 680, 802, 1029, 1146
CviQI GTAC 2 cut(s) 336, 1169
DdeI CTNAG 3 cut(s) 189, 339, 1197
DinI GGCGCC 1 cut(s) 156
DpnI GATC 4 cut(s) 183, 364, 669, 1005
DpnII GATC 4 cut(s) 181, 362, 667, 1003
DraI TTTAAA 1 cut(s) 753
DrdI GACNNNNNNGTC 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 405
DseDI GACNNNNNNGTC 1 cut(s) 861
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 432
Eco130I CCWWGG 5 cut(s) 224, 382, 458, 671, 711
Eco47I GGWCC 4 cut(s) 193, 269, 357, 1046
Eco57I CTGAAG 4 cut(s) 239, 600, 611, 893
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 193
EcoT14I CCWWGG 5 cut(s) 224, 382, 458, 671, 711
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 5 cut(s) 224, 382, 458, 671, 711
FaeI CATG 7 cut(s) 303, 418, 462, 683, 805, 1032, 1149
FaqI GGGAC 2 cut(s) 86, 945
FatI CATG 7 cut(s) 299, 414, 458, 679, 801, 1028, 1145
FauI CCCGC 2 cut(s) 831, 1064
FauNDI CATATG 1 cut(s) 431
FbaI TGATCA 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 32, 601, 696, 723, 726
FokI GGATG 2 cut(s) 548, 1158
Fsp4HI GCNGC 5 cut(s) 32, 601, 696, 723, 726
FspBI CTAG 4 cut(s) 234, 672, 729, 879
GlaI GCGC 3 cut(s) 30, 156, 246
GluI GCNGC 5 cut(s) 32, 601, 696, 723, 726
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 457, 1014
HhaI GCGC 3 cut(s) 31, 157, 247
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 7 cut(s) 303, 418, 462, 683, 805, 1032, 1149
Hin6I GCGC 3 cut(s) 29, 155, 245
HinP1I GCGC 3 cut(s) 29, 155, 245
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 4 cut(s) 40, 108, 143, 440
HphI GGTGA 2 cut(s) 561, 977
Hpy166II GTNNAC 3 cut(s) 374, 514, 562
Hpy188I TCNGA 8 cut(s) 190, 219, 290, 367, 609, 618, 745, 1198
Hpy188III TCNNGA 4 cut(s) 79, 360, 578, 866
Hpy8I GTNNAC 3 cut(s) 374, 514, 562
Hpy99I CGWCG 3 cut(s) 10, 143, 786
HpyAV CCTTC 6 cut(s) 78, 106, 490, 783, 868, 1196
HpyCH4III ACNGT 8 cut(s) 371, 378, 665, 782, 833, 863, 973, 1082
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 6 cut(s) 414, 805, 825, 993, 1110, 1119
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 309, 792, 844, 1116
HpyF3I CTNAG 3 cut(s) 189, 339, 1197
HpySE526I ACGT 1 cut(s) 950
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 7 cut(s) 303, 418, 462, 683, 805, 1032, 1149
HspAI GCGC 3 cut(s) 29, 155, 245
KasI GGCGCC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 1003
Kzo9I GATC 4 cut(s) 181, 362, 667, 1003
LmnI GCTCC 1 cut(s) 792
Lsp1109I GCAGC 2 cut(s) 707, 712
MaeI CTAG 4 cut(s) 234, 672, 729, 879
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 3 cut(s) 109, 567, 767
MalI GATC 4 cut(s) 183, 364, 669, 1005
MbiI CCGCTC 2 cut(s) 603, 1071
MboI GATC 4 cut(s) 181, 362, 667, 1003
MluCI AATT 6 cut(s) 74, 477, 517, 699, 815, 996
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 4 cut(s) 49, 117, 137, 449
MmeI TCCRAC 2 cut(s) 565, 971
MseI TTAA 5 cut(s) 92, 489, 540, 702, 752
MslI CAYNNNNRTG 3 cut(s) 48, 800, 1105
MspA1I CMGCKG 1 cut(s) 725
Mva1269I GAATGC 2 cut(s) 235, 625
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 5 cut(s) 31, 309, 792, 844, 1116
NarI GGCGCC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 458
NdeI CATATG 1 cut(s) 431
NdeII GATC 4 cut(s) 181, 362, 667, 1003
NlaIII CATG 7 cut(s) 303, 418, 462, 683, 805, 1032, 1149
NlaIV GGNNCC 1 cut(s) 156
NmeAIII GCCGAG 1 cut(s) 338
NmuCI GTSAC 3 cut(s) 109, 567, 767
NspI RCATGY 2 cut(s) 1032, 1149
PciI ACATGT 1 cut(s) 1145
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 2 cut(s) 235, 625
PkrI GCNGC 5 cut(s) 33, 602, 697, 724, 727
PleI GAGTC 4 cut(s) 48, 116, 137, 448
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 4 cut(s) 48, 116, 137, 448
PpuMI RGGWCCY 1 cut(s) 193
PscI ACATGT 1 cut(s) 1145
Psp1406I AACGTT 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 4 cut(s) 193, 269, 357, 1046
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 827
PstNI CAGNNNCTG 1 cut(s) 831
RsaI GTAC 2 cut(s) 337, 1170
RsaNI GTAC 2 cut(s) 336, 1169
RseI CAYNNNNRTG 3 cut(s) 48, 800, 1105
SaqAI TTAA 5 cut(s) 92, 489, 540, 702, 752
SatI GCNGC 5 cut(s) 32, 601, 696, 723, 726
Sau3AI GATC 4 cut(s) 181, 362, 667, 1003
Sau96I GGNCC 4 cut(s) 193, 269, 357, 1046
SchI GAGTC 4 cut(s) 49, 117, 137, 449
SfcI CTRYAG 1 cut(s) 823
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 4 cut(s) 193, 269, 357, 1046
SmiMI CAYNNNNRTG 3 cut(s) 48, 800, 1105
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 6 cut(s) 74, 477, 517, 699, 815, 996
SsiI CCGC 5 cut(s) 32, 601, 723, 838, 1071
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 4 cut(s) 234, 672, 729, 879
StyI CCWWGG 5 cut(s) 224, 382, 458, 671, 711
TaaI ACNGT 8 cut(s) 371, 378, 665, 782, 833, 863, 973, 1082
TaiI ACGT 1 cut(s) 953
TaqI TCGA 2 cut(s) 443, 1021
TaqII GACCGA 1 cut(s) 257
TasI AATT 6 cut(s) 74, 477, 517, 699, 815, 996
TatI WGTACW 2 cut(s) 335, 1168
TauI GCSGC 3 cut(s) 34, 603, 725
Tru1I TTAA 5 cut(s) 92, 489, 540, 702, 752
Tru9I TTAA 5 cut(s) 92, 489, 540, 702, 752
TscAI CASTG 3 cut(s) 383, 416, 976
TseFI GTSAC 3 cut(s) 109, 567, 767
TseI GCWGC 2 cut(s) 695, 725
Tsp45I GTSAC 3 cut(s) 109, 567, 767
TspDTI ATGAA 6 cut(s) 465, 522, 665, 668, 889, 902
TspRI CASTG 3 cut(s) 383, 416, 976
VpaK11BI GGWCC 4 cut(s) 193, 269, 357, 1046
XagI CCTNNNNNAGG 1 cut(s) 386
XapI RAATTY 3 cut(s) 74, 815, 996
XceI RCATGY 2 cut(s) 1032, 1149
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 4 cut(s) 234, 672, 729, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.