FvH4_4g31851

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013263
Physical Location & Seq
Forward (+)
30790072 .. 30793136
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31851.t1

Sequence Viewer

Length: 1206 bp
ATGTCGTCGTTGTGTTCGTTAGGGCTTGCGCCGCAGTGGAGGCATGAGAGCGTGAGCTTCTTTCTCTTATTCAAATTCAAGAGAAGGACTTTAACCTTCGTCCCAAGAGTTACTTCTTCCTCTTTAAACGACGACGACGACTCTGTTTCGGCGCCAAAACCAAAGCAGTTGGGTTATGATCTTTCAGAGGACCTGTTCGGGCTTGAGGGTAATCCAAAGCCAAGGAATGCTACTTTGGGTGCACGCAAACCAAGGTCCTGGTTTGGTCCAAATGGTCAGTACATCAGGGAGCTACCATGCCCGAGTTGCCGAGGGAGGGGTTATACTCCTTGTACTGAGTGTGGTATAGAAAGGTCCAGATCAGACTGTTCACAGTGCCTTGGCAAGGGCATAATGACTTGTCGACAGTGCTTGGGAGATTGTGTCATATGGGAAGAGTCAATTGATGAACGGCCATGGGAGAAAGCTCGTTTAATTTCTCCACTCAAAGTGAAAGAAGATGATGAAGTTGATAATTTGGAAATAAAACCGAATGTCAAGAGAAAGTCAAAGCGAGTATACCAGTCACCACCTCCTGAAGTTAATCTGAAGATTAGCCGGTCTCTGAAAAGTCTGAATGCCAAAACTGGTCTATTTAGTAAGAGAATGAAGATCATCCACCGTGATCCTAGGCTTCATGCTCAAAGAGTGGCTGCAATTAAGAAAGCCAAGGGAACTGCCGCAGCTAGAAAGCATACGTCTGAAACTTTAAAAGCTTTCTTCAGTGACCCAGAAAACCGTCGCAAAAGGAGCATTTCCATGCAGGGCATAAAGTTTTACTGCAGAAACTGTGGACGGGAGGGGCATAGGAGACACTACTGTCCAGAACTGAAGGCTAGCTTGATTAATAGGCAGTTCATCTGTGGCGTCTGTGGAGAAAAGGGTCATAACAGAAGAACCTGTCCGACATTGAACCGTCCCAAAACCCTTGTTCGAAGACCTCACCACTGCAGAATATGTGATCAAAGAGGCCACAATCGAACGACATGCCCCAAAGTGATTGGACGAAAGTCCAGTATTGATGTAAAGAAAGGGTTTCACAGTTCTGGAAACCGAAGAACATACAAGTGTACGTTGTGCAAAGAAAATGGGCATAATAGTAGGACATGTCCTACTCATAACAAAAATGTACATTCCTCAAACATTGGTCTTCAATCTGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_2

Protein Analysis

402

Amino Acids

45.64

Weight (kDa)

9.94

Isoelectric Point (pI)

58.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20220 AT5G20220 AT5G20220 AT5G20220
fragaria_vesca FvH4_4g31851
malus_domestica MD13G1078100.v1.1
prunus_persica Prupe.1G269000_v2.0.a1
pyrus_communis pycom13g06760
rosa_chinensis RchiOBHm_Chr4g0440521
rosa_laevigata RLG00000006172
rosa_roxburghii Rroxscaffold_5G00381350
rosa_rugosa Rorug04G0326400
rosa_samantha Rh4AG378100 Rh4BG390700 Rh4CG405400 Rh4DG384400
rosa_wichuraiana Rw4G032360 Rw4G032460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 858
AccB1I GGYRCC 1 cut(s) 151
AccI GTMKAC 2 cut(s) 403, 558
AciI CCGC 2 cut(s) 32, 720
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 74
AcuI CTGAAG 4 cut(s) 597, 608, 745, 890
AcyI GRCGYC 2 cut(s) 152, 906
AfaI GTAC 4 cut(s) 281, 334, 1111, 1170
AfiI CCNNNNNNNGG 2 cut(s) 316, 385
AflIII ACRYGT 1 cut(s) 1145
AgsI TTSAA 4 cut(s) 73, 79, 952, 1193
AjnI CCWGG 1 cut(s) 257
AjuI GAANNNNNNNTTGG 4 cut(s) 97, 129, 218, 250
AluBI AGCT 6 cut(s) 57, 292, 467, 725, 755, 879
AluI AGCT 6 cut(s) 57, 292, 467, 725, 755, 879
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 2 cut(s) 606, 844
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 828
Ama87I CYCGRG 1 cut(s) 301
AoxI GGCC 2 cut(s) 452, 1009
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 2 cut(s) 692, 722
ApoI RAATTY 1 cut(s) 74
AseI ATTAAT 1 cut(s) 885
AspA2I CCTAGG 1 cut(s) 668
AspLEI GCGC 2 cut(s) 31, 154
AspS9I GGNCC 4 cut(s) 190, 255, 266, 354
AsuHPI GGTGA 2 cut(s) 558, 974
AsuII TTCGAA 1 cut(s) 973
AsuNHI GCTAGC 1 cut(s) 875
AvaI CYCGRG 1 cut(s) 301
AvaII GGWCC 4 cut(s) 190, 255, 266, 354
AvrII CCTAGG 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 982, 1181
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 679, 734
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 1008, 1042
BciT130I CCWGG 1 cut(s) 259
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 2 cut(s) 606, 844
BfaI CTAG 3 cut(s) 669, 726, 876
BfmI CTRYAG 2 cut(s) 820, 988
BfoI RGCGCY 1 cut(s) 155
BisI GCNGC 4 cut(s) 32, 693, 720, 723
BlnI CCTAGG 1 cut(s) 668
BlsI GCNGC 4 cut(s) 33, 694, 721, 724
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 4 cut(s) 190, 255, 266, 354
BmeT110I CYCGRG 1 cut(s) 301
BmgT120I GGNCC 4 cut(s) 190, 255, 266, 354
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 1 cut(s) 259
BmtI GCTAGC 1 cut(s) 879
BoxI GACNNNNGTC 1 cut(s) 1048
BpiI GAAGAC 2 cut(s) 982, 1181
Bpu14I TTCGAA 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 152, 906
BsaI GGTCTC 1 cut(s) 606
BsaJI CCNNGG 7 cut(s) 221, 251, 310, 379, 455, 668, 708
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 385
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 3 cut(s) 562, 631, 1053
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 7 cut(s) 221, 251, 310, 379, 455, 668, 708
BseGI GGATG 1 cut(s) 654
BseLI CCNNNNNNNGG 2 cut(s) 316, 385
BseMII CTCAG 2 cut(s) 327, 1188
BseNI ACTGG 3 cut(s) 562, 631, 1053
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 2 cut(s) 679, 734
BshFI GGCC 2 cut(s) 454, 1011
BshNI GGYRCC 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 301
BsiSI CCGG 1 cut(s) 598
BslFI GGGAC 2 cut(s) 86, 942
BslI CCNNNNNNNGG 2 cut(s) 316, 385
BsmAI GTCTC 2 cut(s) 606, 844
BsmFI GGGAC 2 cut(s) 86, 942
BsmI GAATGC 2 cut(s) 232, 622
BsnI GGCC 2 cut(s) 454, 1011
Bso31I GGTCTC 1 cut(s) 606
BsoBI CYCGRG 1 cut(s) 301
Bsp119I TTCGAA 1 cut(s) 973
Bsp1286I GDGCHC 1 cut(s) 244
Bsp1407I TGTACA 1 cut(s) 1168
Bsp143I GATC 5 cut(s) 178, 359, 651, 664, 1000
Bsp19I CCATGG 1 cut(s) 455
BspACI CCGC 2 cut(s) 32, 720
BspANI GGCC 2 cut(s) 454, 1011
BspCNI CTCAG 2 cut(s) 328, 1189
BspLI GGNNCC 1 cut(s) 153
BspMAI CTGCAG 2 cut(s) 824, 992
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 973
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 1168
BsrI ACTGG 3 cut(s) 562, 631, 1053
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 7 cut(s) 221, 251, 310, 379, 455, 668, 708
BssMI GATC 5 cut(s) 178, 359, 651, 664, 1000
BssNAI GTATAC 1 cut(s) 559
BssNI GRCGYC 2 cut(s) 152, 906
BssT1I CCWWGG 6 cut(s) 221, 251, 379, 455, 668, 708
Bst1107I GTATAC 1 cut(s) 559
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 9 cut(s) 368, 375, 408, 662, 779, 830, 860, 956, 1082
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 2 cut(s) 152, 906
BstAUI TGTACA 1 cut(s) 1168
BstBI TTCGAA 1 cut(s) 973
BstC8I GCNNGC 3 cut(s) 27, 244, 877
BstDEI CTNAG 2 cut(s) 336, 1197
BstDSI CCRYGG 1 cut(s) 455
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 654
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 2 cut(s) 31, 154
BstKTI GATC 5 cut(s) 181, 362, 654, 667, 1003
BstMAI GTCTC 2 cut(s) 606, 844
BstMBI GATC 5 cut(s) 178, 359, 651, 664, 1000
BstMWI GCNNNNNNNGC 4 cut(s) 31, 40, 306, 789
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 2 cut(s) 1029, 1149
BstPAI GACNNNNGTC 1 cut(s) 1048
BstSCI CCNGG 1 cut(s) 257
BstSFI CTRYAG 2 cut(s) 820, 988
BstSLI GKGCMC 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 679, 734
BstV2I GAAGAC 2 cut(s) 982, 1181
BstXI CCANNNNNNTGG 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 559
BsuRI GGCC 2 cut(s) 454, 1011
BtgI CCRYGG 1 cut(s) 455
BtsCI GGATG 1 cut(s) 654
BtsI GCAGTG 2 cut(s) 41, 985
BtsIMutI CAGTG 5 cut(s) 41, 380, 413, 769, 985
Cac8I GCNNGC 3 cut(s) 27, 244, 877
CaiI CAGNNNCTG 1 cut(s) 828
CfoI GCGC 2 cut(s) 31, 154
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 4 cut(s) 190, 255, 266, 354
CseI GACGC 1 cut(s) 895
Csp6I GTAC 4 cut(s) 280, 333, 1110, 1169
CviAII CATG 7 cut(s) 44, 297, 456, 677, 799, 1026, 1146
CviQI GTAC 4 cut(s) 280, 333, 1110, 1169
DdeI CTNAG 2 cut(s) 336, 1197
DinI GGCGCC 1 cut(s) 153
DpnI GATC 5 cut(s) 180, 361, 653, 666, 1002
DpnII GATC 5 cut(s) 178, 359, 651, 664, 1000
DraI TTTAAA 2 cut(s) 126, 750
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 6 cut(s) 221, 251, 379, 455, 668, 708
Eco31I GGTCTC 1 cut(s) 606
Eco47I GGWCC 4 cut(s) 190, 255, 266, 354
Eco57I CTGAAG 4 cut(s) 597, 608, 745, 890
Eco88I CYCGRG 1 cut(s) 301
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoO109I RGGNCCY 2 cut(s) 190, 255
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 6 cut(s) 221, 251, 379, 455, 668, 708
EgeI GGCGCC 1 cut(s) 153
EheI GGCGCC 1 cut(s) 153
ErhI CCWWGG 6 cut(s) 221, 251, 379, 455, 668, 708
FaeI CATG 7 cut(s) 47, 300, 459, 680, 802, 1029, 1149
FalI AAGNNNNNCTT 4 cut(s) 97, 129, 863, 895
FaqI GGGAC 2 cut(s) 86, 942
FatI CATG 7 cut(s) 43, 296, 455, 676, 798, 1025, 1145
FauNDI CATATG 1 cut(s) 428
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 2 cut(s) 403, 558
Fnu4HI GCNGC 4 cut(s) 32, 693, 720, 723
FokI GGATG 1 cut(s) 641
Fsp4HI GCNGC 4 cut(s) 32, 693, 720, 723
FspBI CTAG 3 cut(s) 669, 726, 876
GlaI GCGC 2 cut(s) 30, 153
GluI GCNGC 4 cut(s) 32, 693, 720, 723
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 2 cut(s) 454, 1011
HapII CCGG 1 cut(s) 598
HgaI GACGC 1 cut(s) 895
HhaI GCGC 2 cut(s) 31, 154
Hin1I GRCGYC 2 cut(s) 152, 906
Hin1II CATG 7 cut(s) 47, 300, 459, 680, 802, 1029, 1149
Hin6I GCGC 2 cut(s) 29, 152
HinP1I GCGC 2 cut(s) 29, 152
HincII GTYRAC 1 cut(s) 404
HindII GTYRAC 1 cut(s) 404
HindIII AAGCTT 1 cut(s) 753
HinfI GANTC 2 cut(s) 140, 437
HpaII CCGG 1 cut(s) 598
HphI GGTGA 2 cut(s) 558, 974
Hpy166II GTNNAC 6 cut(s) 242, 371, 404, 559, 833, 1110
Hpy188I TCNGA 8 cut(s) 187, 364, 588, 606, 615, 742, 945, 1198
Hpy188III TCNNGA 6 cut(s) 79, 357, 538, 575, 863, 1086
Hpy8I GTNNAC 6 cut(s) 242, 371, 404, 559, 833, 1110
Hpy99I CGWCG 5 cut(s) 10, 134, 137, 140, 783
HpyAV CCTTC 3 cut(s) 78, 106, 865
HpyCH4III ACNGT 9 cut(s) 368, 375, 408, 662, 779, 830, 860, 956, 1082
HpyCH4IV ACGT 2 cut(s) 737, 1112
HpyCH4V TGCA 6 cut(s) 242, 695, 802, 822, 990, 1119
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 40, 306, 789
HpyF3I CTNAG 2 cut(s) 336, 1197
HpySE526I ACGT 2 cut(s) 737, 1112
Hsp92I GRCGYC 2 cut(s) 152, 906
Hsp92II CATG 7 cut(s) 47, 300, 459, 680, 802, 1029, 1149
HspAI GCGC 2 cut(s) 29, 152
KasI GGCGCC 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 5 cut(s) 178, 359, 651, 664, 1000
LmnI GCTCC 2 cut(s) 289, 789
Lsp1109I GCAGC 2 cut(s) 679, 734
MaeI CTAG 3 cut(s) 669, 726, 876
MaeII ACGT 2 cut(s) 737, 1112
MaeIII GTNAC 3 cut(s) 109, 564, 764
MalI GATC 5 cut(s) 180, 361, 653, 666, 1002
MboI GATC 5 cut(s) 178, 359, 651, 664, 1000
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 5 cut(s) 74, 441, 474, 514, 696
Mly113I GGCGCC 1 cut(s) 152
MlyI GAGTC 2 cut(s) 134, 446
MmeI TCCRAC 1 cut(s) 968
MseI TTAA 7 cut(s) 92, 125, 473, 582, 699, 749, 885
MslI CAYNNNNRTG 2 cut(s) 797, 1105
MspI CCGG 1 cut(s) 598
MspR9I CCNGG 1 cut(s) 259
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 2 cut(s) 232, 622
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 4 cut(s) 31, 40, 306, 789
NarI GGCGCC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 455
NdeI CATATG 1 cut(s) 428
NdeII GATC 5 cut(s) 178, 359, 651, 664, 1000
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 7 cut(s) 47, 300, 459, 680, 802, 1029, 1149
NlaIV GGNNCC 1 cut(s) 153
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 2 cut(s) 564, 764
NspI RCATGY 2 cut(s) 1029, 1149
NspV TTCGAA 1 cut(s) 973
PciI ACATGT 1 cut(s) 1145
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 135
PctI GAATGC 2 cut(s) 232, 622
PkrI GCNGC 4 cut(s) 33, 694, 721, 724
PleI GAGTC 2 cut(s) 134, 445
PluTI GGCGCC 1 cut(s) 155
PpsI GAGTC 2 cut(s) 134, 445
PpuMI RGGWCCY 2 cut(s) 190, 255
PscI ACATGT 1 cut(s) 1145
PshAI GACNNNNGTC 1 cut(s) 1048
PshBI ATTAAT 1 cut(s) 885
Psp5II RGGWCCY 2 cut(s) 190, 255
Psp6I CCWGG 1 cut(s) 257
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 4 cut(s) 190, 255, 266, 354
PspPPI RGGWCCY 2 cut(s) 190, 255
PstI CTGCAG 2 cut(s) 824, 992
PstNI CAGNNNCTG 1 cut(s) 828
RsaI GTAC 4 cut(s) 281, 334, 1111, 1170
RsaNI GTAC 4 cut(s) 280, 333, 1110, 1169
RseI CAYNNNNRTG 2 cut(s) 797, 1105
SalI GTCGAC 1 cut(s) 402
SaqAI TTAA 7 cut(s) 92, 125, 473, 582, 699, 749, 885
SatI GCNGC 4 cut(s) 32, 693, 720, 723
Sau3AI GATC 5 cut(s) 178, 359, 651, 664, 1000
Sau96I GGNCC 4 cut(s) 190, 255, 266, 354
SchI GAGTC 2 cut(s) 134, 446
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 244
SfcI CTRYAG 2 cut(s) 820, 988
SfoI GGCGCC 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 973
SinI GGWCC 4 cut(s) 190, 255, 266, 354
SmiMI CAYNNNNRTG 2 cut(s) 797, 1105
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 5 cut(s) 74, 441, 474, 514, 696
SsiI CCGC 2 cut(s) 32, 720
SspDI GGCGCC 1 cut(s) 151
SspMI CTAG 3 cut(s) 669, 726, 876
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 6 cut(s) 221, 251, 379, 455, 668, 708
TaaI ACNGT 9 cut(s) 368, 375, 408, 662, 779, 830, 860, 956, 1082
TaiI ACGT 2 cut(s) 740, 1115
TaqI TCGA 3 cut(s) 403, 973, 1018
TasI AATT 5 cut(s) 74, 441, 474, 514, 696
TatI WGTACW 3 cut(s) 279, 332, 1168
TauI GCSGC 2 cut(s) 34, 722
Tru1I TTAA 7 cut(s) 92, 125, 473, 582, 699, 749, 885
Tru9I TTAA 7 cut(s) 92, 125, 473, 582, 699, 749, 885
TscAI CASTG 5 cut(s) 41, 380, 413, 769, 992
TseFI GTSAC 2 cut(s) 564, 764
TseI GCWGC 2 cut(s) 692, 722
Tsp45I GTSAC 2 cut(s) 564, 764
TspDTI ATGAA 5 cut(s) 462, 519, 662, 665, 886
TspRI CASTG 5 cut(s) 41, 380, 413, 769, 992
VneI GTGCAC 1 cut(s) 240
VpaK11BI GGWCC 4 cut(s) 190, 255, 266, 354
VspI ATTAAT 1 cut(s) 885
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 74
XceI RCATGY 2 cut(s) 1029, 1149
XmaJI CCTAGG 1 cut(s) 668
XmiI GTMKAC 2 cut(s) 403, 558
XspI CTAG 3 cut(s) 669, 726, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.