AT5G20270

Heptahelical transmembrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008678
Physical Location & Seq
Reverse (-)
6840833 .. 6842946
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20270.1

Sequence Viewer

Length: 999 bp
ATGGACCAAAATGGTCATAACGACGAAGCAGAAACAGTTTCATGCGGCAACGGCAACTGCAAAAGCAAGATCGTGCCTGGAGATGATCACGGTGGAGATGAGAGTAGTGGTACCAAGAGAAGGAAGAAGAGAAAAACGCAACAAAAGACAATGAAACGACGTGAATTAATGTCGTATTGTGAGCTACCGGAGTACATGAAAGACAACGAATACATATTGAATTATTACAGAGCTGATTGGTCCATTAGAGATGCTTTCTTCAGCGTATTTAGTTTCCACAATGAGTCCCTCAATGTTTGGACACATTTGATTGGTTTTATCTTCTTTGTGGCACTAACCGTCGCCAATATTATTCATCACGACGGATTCTTCCCCGTGGATGCAAAGAGTCCAGGGAATGTAACAAGATGGCCCTTCTTCGTTTTCTTAGGAGGCTCAATGTTCTGCCTACTCGCAAGCAGCATTTGTCACCTCTTCTGTTGCCACTCAAAAGAGCTTAATGTCTTCCTTCTCCGAATAGACTATGCCGGTATCACCGCCATGATCATCACTTCCTTCTTCCCACCAATCTTCTACATCTTCCAGTGCACTCCTCGGTGGTACTTCATCTACCTCGCAGGCATCACATCCATGGGAATCTTCACAATCATCACACTTTTCACCCCATCACTTTCCGCTCCCAAGTACCGAGCTTTCCGCGCTTTGCTCTTTGCCTCCATGGGGCTGTTTGGGATTGTCCCAGCTGCCCACGCGCTAGTGGTCAATTGGGGAAACCCGCAAAGGAACGTGACGCTCGTGTACGAGTTGCTTATGGCAGTGTTCTATCTTGTTGGAACAGGATTCTACGTAGGGAGAGTGCCAGAAAGGCTTAAACCGGGATGGTTTGATCGTGTGGGACATAGTCATCAGATCTTCCATGTGTTCGTTTTGTTGGGTGCTTTGTCTCACTATGCAGCTGCTCTCTTGTTCTTGGACTGGCGTGACCACGTTGGTTGTTAA

Protein Analysis

332

Amino Acids

37.88

Weight (kDa)

8.67

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 90 - 317 5.6e-65 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 677
AccII CGCG 2 cut(s) 699, 752
AciI CCGC 5 cut(s) 45, 537, 675, 697, 776
AcuI CTGAAG 1 cut(s) 244
AfaI GTAC 5 cut(s) 112, 194, 602, 686, 800
AfiI CCNNNNNNNGG 2 cut(s) 120, 720
AgsI TTSAA 1 cut(s) 220
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 2 cut(s) 76, 391
AluBI AGCT 6 cut(s) 184, 233, 496, 692, 743, 956
AluI AGCT 6 cut(s) 184, 233, 496, 692, 743, 956
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 1 cut(s) 948
Alw44I GTGCAC 1 cut(s) 586
AoxI GGCC 1 cut(s) 410
ApaLI GTGCAC 1 cut(s) 586
ApeKI GCWGC 4 cut(s) 459, 743, 953, 956
AseI ATTAAT 1 cut(s) 167
Asp718I GGTACC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 701, 754
AspS9I GGNCC 3 cut(s) 4, 240, 411
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 3 cut(s) 461, 526, 652
AvaII GGWCC 2 cut(s) 4, 240
BaeGI GKGCMC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 110
BauI CACGAG 1 cut(s) 794
BbsI GAAGAC 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 590
BbvI GCAGC 4 cut(s) 471, 730, 943, 965
BccI CCATC 3 cut(s) 402, 673, 873
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 2 cut(s) 78, 393
BclI TGATCA 2 cut(s) 85, 543
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 1 cut(s) 948
BfaI CTAG 1 cut(s) 755
BglI GCCNNNNNGGC 1 cut(s) 865
BglII AGATCT 1 cut(s) 909
BisI GCNGC 5 cut(s) 46, 460, 744, 954, 957
BlsI GCNGC 5 cut(s) 47, 461, 745, 955, 958
Bme1390I CCNGG 3 cut(s) 78, 393, 876
Bme18I GGWCC 2 cut(s) 4, 240
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 4, 240, 411
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 3 cut(s) 78, 393, 876
BmsI GCATC 3 cut(s) 241, 370, 630
BpiI GAAGAC 1 cut(s) 496
BpmI CTGGAG 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 847
BsaJI CCNNGG 5 cut(s) 375, 392, 593, 630, 717
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 720
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 2 cut(s) 583, 980
BseBI CCWGG 2 cut(s) 78, 393
BseDI CCNNGG 5 cut(s) 375, 392, 593, 630, 717
BseGI GGATG 3 cut(s) 385, 626, 884
BseLI CCNNNNNNNGG 2 cut(s) 120, 720
BseNI ACTGG 2 cut(s) 583, 980
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 4 cut(s) 471, 730, 943, 965
BseYI CCCAGC 1 cut(s) 739
Bsh1236I CGCG 2 cut(s) 699, 752
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 3 cut(s) 188, 528, 875
BslFI GGGAC 3 cut(s) 271, 722, 909
BslI CCNNNNNNNGG 2 cut(s) 120, 720
BsmAI GTCTC 1 cut(s) 948
BsmFI GGGAC 3 cut(s) 271, 722, 909
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 590
Bsp143I GATC 5 cut(s) 69, 85, 543, 886, 909
Bsp19I CCATGG 2 cut(s) 630, 717
BspACI CCGC 5 cut(s) 45, 537, 675, 697, 776
BspANI GGCC 1 cut(s) 412
BspFNI CGCG 2 cut(s) 699, 752
BspLI GGNNCC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 2 cut(s) 583, 980
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 5 cut(s) 375, 392, 593, 630, 717
BssMI GATC 5 cut(s) 69, 85, 543, 886, 909
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 630, 717
Bst2BI CACGAG 1 cut(s) 794
Bst2UI CCWGG 2 cut(s) 78, 393
Bst4CI ACNGT 3 cut(s) 37, 92, 340
Bst6I CTCTTC 2 cut(s) 122, 479
BstBAI YACGTR 1 cut(s) 847
BstC8I GCNNGC 2 cut(s) 457, 619
BstDEI CTNAG 1 cut(s) 427
BstDSI CCRYGG 3 cut(s) 375, 630, 717
BstF5I GGATG 3 cut(s) 385, 626, 884
BstFNI CGCG 2 cut(s) 699, 752
BstHHI GCGC 2 cut(s) 701, 754
BstKTI GATC 5 cut(s) 72, 88, 546, 889, 912
BstMAI GTCTC 1 cut(s) 948
BstMBI GATC 5 cut(s) 69, 85, 543, 886, 909
BstMWI GCNNNNNNNGC 4 cut(s) 51, 698, 749, 865
BstNI CCWGG 2 cut(s) 78, 393
BstSCI CCNGG 3 cut(s) 76, 391, 874
BstSLI GKGCMC 1 cut(s) 590
BstSNI TACGTA 1 cut(s) 847
BstUI CGCG 2 cut(s) 699, 752
BstV1I GCAGC 4 cut(s) 471, 730, 943, 965
BstV2I GAAGAC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 909
BstYI RGATCY 1 cut(s) 909
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 3 cut(s) 375, 630, 717
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 3 cut(s) 385, 626, 884
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 2 cut(s) 590, 822
Cac8I GCNNGC 2 cut(s) 457, 619
CfoI GCGC 2 cut(s) 701, 754
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 3 cut(s) 4, 240, 411
CseI GACGC 1 cut(s) 799
Csp6I GTAC 5 cut(s) 111, 193, 601, 685, 799
CspCI CAANNNNNGTGG 1 cut(s) 974
CviAII CATG 6 cut(s) 42, 196, 541, 631, 718, 917
CviQI GTAC 5 cut(s) 111, 193, 601, 685, 799
DdeI CTNAG 1 cut(s) 427
DpnI GATC 5 cut(s) 71, 87, 545, 888, 911
DpnII GATC 5 cut(s) 69, 85, 543, 886, 909
Eam1104I CTCTTC 2 cut(s) 122, 479
EarI CTCTTC 2 cut(s) 122, 479
Eco105I TACGTA 1 cut(s) 847
Eco130I CCWWGG 2 cut(s) 630, 717
Eco47I GGWCC 2 cut(s) 4, 240
Eco57I CTGAAG 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 76, 391
EcoT14I CCWWGG 2 cut(s) 630, 717
ErhI CCWWGG 2 cut(s) 630, 717
FaeI CATG 6 cut(s) 45, 199, 544, 634, 721, 920
FaqI GGGAC 3 cut(s) 271, 722, 909
FatI CATG 6 cut(s) 41, 195, 540, 630, 717, 916
FauI CCCGC 1 cut(s) 783
FbaI TGATCA 2 cut(s) 85, 543
Fnu4HI GCNGC 5 cut(s) 46, 460, 744, 954, 957
FokI GGATG 3 cut(s) 392, 613, 891
Fsp4HI GCNGC 5 cut(s) 46, 460, 744, 954, 957
FspBI CTAG 1 cut(s) 755
GlaI GCGC 2 cut(s) 700, 753
GluI GCNGC 5 cut(s) 46, 460, 744, 954, 957
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 1 cut(s) 412
HapII CCGG 3 cut(s) 188, 528, 875
HgaI GACGC 1 cut(s) 799
HhaI GCGC 2 cut(s) 701, 754
Hin1II CATG 6 cut(s) 45, 199, 544, 634, 721, 920
Hin6I GCGC 2 cut(s) 699, 752
HinP1I GCGC 2 cut(s) 699, 752
HinfI GANTC 5 cut(s) 284, 366, 388, 636, 840
HpaII CCGG 3 cut(s) 188, 528, 875
HphI GGTGA 3 cut(s) 461, 526, 652
Hpy166II GTNNAC 2 cut(s) 588, 799
Hpy188I TCNGA 2 cut(s) 515, 909
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 588, 799
Hpy99I CGWCG 4 cut(s) 26, 162, 344, 365
HpyAV CCTTC 4 cut(s) 114, 424, 518, 565
HpyCH4III ACNGT 3 cut(s) 37, 92, 340
HpyCH4IV ACGT 4 cut(s) 160, 786, 846, 987
HpyCH4V TGCA 4 cut(s) 60, 383, 588, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 698, 749, 865
HpyF3I CTNAG 1 cut(s) 427
HpySE526I ACGT 4 cut(s) 160, 786, 846, 987
Hsp92II CATG 6 cut(s) 45, 199, 544, 634, 721, 920
HspAI GCGC 2 cut(s) 699, 752
KpnI GGTACC 1 cut(s) 114
Ksp22I TGATCA 2 cut(s) 85, 543
Kzo9I GATC 5 cut(s) 69, 85, 543, 886, 909
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 4 cut(s) 471, 730, 943, 965
LweI GCATC 3 cut(s) 241, 370, 630
MaeI CTAG 1 cut(s) 755
MaeII ACGT 4 cut(s) 160, 786, 846, 987
MaeIII GTNAC 4 cut(s) 400, 467, 787, 980
MalI GATC 5 cut(s) 71, 87, 545, 888, 911
MbiI CCGCTC 1 cut(s) 677
MboI GATC 5 cut(s) 69, 85, 543, 886, 909
MfeI CAATTG 1 cut(s) 763
MflI RGATCY 1 cut(s) 909
MhlI GDGCHC 1 cut(s) 590
MluCI AATT 3 cut(s) 164, 220, 763
MlyI GAGTC 2 cut(s) 293, 397
MmeI TCCRAC 1 cut(s) 811
MnlI CCTC 6 cut(s) 299, 425, 482, 603, 623, 724
MseI TTAA 4 cut(s) 167, 498, 870, 997
MslI CAYNNNNRTG 2 cut(s) 539, 629
MspA1I CMGCKG 2 cut(s) 743, 956
MspI CCGG 3 cut(s) 188, 528, 875
MspR9I CCNGG 3 cut(s) 78, 393, 876
MunI CAATTG 1 cut(s) 763
MvaI CCWGG 2 cut(s) 78, 393
MvnI CGCG 2 cut(s) 699, 752
MwoI GCNNNNNNNGC 4 cut(s) 51, 698, 749, 865
NciI CCSGG 1 cut(s) 876
NcoI CCATGG 2 cut(s) 630, 717
NdeII GATC 5 cut(s) 69, 85, 543, 886, 909
NlaIII CATG 6 cut(s) 45, 199, 544, 634, 721, 920
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 3 cut(s) 467, 787, 980
PcsI WCGNNNNNNNCGW 1 cut(s) 792
PfeI GAWTC 3 cut(s) 366, 636, 840
PflFI GACNNNGTC 1 cut(s) 900
PkrI GCNGC 5 cut(s) 47, 461, 745, 955, 958
PleI GAGTC 2 cut(s) 292, 396
PpsI GAGTC 2 cut(s) 292, 396
Ppu21I YACGTR 1 cut(s) 847
PshBI ATTAAT 1 cut(s) 167
Psp6I CCWGG 2 cut(s) 76, 391
PspFI CCCAGC 1 cut(s) 739
PspGI CCWGG 2 cut(s) 76, 391
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 3 cut(s) 4, 240, 411
PsuI RGATCY 1 cut(s) 909
PsyI GACNNNGTC 1 cut(s) 900
PvuII CAGCTG 2 cut(s) 743, 956
RsaI GTAC 5 cut(s) 112, 194, 602, 686, 800
RsaNI GTAC 5 cut(s) 111, 193, 601, 685, 799
RseI CAYNNNNRTG 2 cut(s) 539, 629
SaqAI TTAA 4 cut(s) 167, 498, 870, 997
SatI GCNGC 5 cut(s) 46, 460, 744, 954, 957
Sau3AI GATC 5 cut(s) 69, 85, 543, 886, 909
Sau96I GGNCC 3 cut(s) 4, 240, 411
SchI GAGTC 2 cut(s) 293, 397
ScrFI CCNGG 3 cut(s) 78, 393, 876
SduI GDGCHC 1 cut(s) 590
SfaNI GCATC 3 cut(s) 241, 370, 630
SinI GGWCC 2 cut(s) 4, 240
SmiMI CAYNNNNRTG 2 cut(s) 539, 629
SnaBI TACGTA 1 cut(s) 847
Sse9I AATT 3 cut(s) 164, 220, 763
SsiI CCGC 5 cut(s) 45, 537, 675, 697, 776
SspI AATATT 1 cut(s) 349
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 3 cut(s) 76, 391, 874
StyI CCWWGG 2 cut(s) 630, 717
TaaI ACNGT 3 cut(s) 37, 92, 340
TaiI ACGT 4 cut(s) 163, 789, 849, 990
TasI AATT 3 cut(s) 164, 220, 763
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 3 cut(s) 366, 636, 840
Tru1I TTAA 4 cut(s) 167, 498, 870, 997
Tru9I TTAA 4 cut(s) 167, 498, 870, 997
TscAI CASTG 2 cut(s) 590, 822
TseFI GTSAC 3 cut(s) 467, 787, 980
TseI GCWGC 4 cut(s) 459, 743, 953, 956
Tsp45I GTSAC 3 cut(s) 467, 787, 980
TspDTI ATGAA 5 cut(s) 30, 167, 212, 344, 595
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 2 cut(s) 590, 822
Tth111I GACNNNGTC 1 cut(s) 900
VneI GTGCAC 1 cut(s) 586
VpaK11BI GGWCC 2 cut(s) 4, 240
VspI ATTAAT 1 cut(s) 167
XspI CTAG 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.