AT5G20995

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004178
Physical Location & Seq
Forward (+)
7134316 .. 7135004
689 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20995.1

Sequence Viewer

Length: 351 bp
ATGATACCATATACTTCGTATATGTTGTCACGCAATCCATATACCTGCCTTATCTACATATACTCATCTTTGACATGTGTCAAACATTTATTTTTTCAGGACTTCTCGGGGAAGACAAGAGATTTTTCAAATGAAGATACAGATCCGTTGTCTAATGAGAAACTCATAGATTTAGGTCTCGAAGAAAGAGAAGCTGCCACAGCTAATCTTAAGGGCATTAGACGTCTTGTGTTAAGTCCTGCCTCATTGATTTCCAAAGGGATATATAGTACTCAGTCTTTCCAGATGGATGAAGAGGAAGGCTTTGGAAGGTCTCTTATTGTTATTGATATTACTAGTATAGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

116

Amino Acids

13.13

Weight (kDa)

4.89

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASY3-like PF20435 66 - 108 9e-07 Meiosis-specific protein ASY3-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 53
AclWI GGATC 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 223
AfaI GTAC 1 cut(s) 271
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 1 cut(s) 129
AhlI ACTAGT 1 cut(s) 335
AluBI AGCT 2 cut(s) 194, 203
AluI AGCT 2 cut(s) 194, 203
Alw26I GTCTC 2 cut(s) 182, 318
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 182, 318
BcuI ACTAGT 1 cut(s) 335
BfaI CTAG 1 cut(s) 336
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 53
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 106
BoxI GACNNNNGTC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 223
BsaI GGTCTC 2 cut(s) 182, 318
Bse8I GATNNNNATC 1 cut(s) 141
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 141
BseMII CTCAG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 106
BsmAI GTCTC 2 cut(s) 182, 318
Bso31I GGTCTC 2 cut(s) 182, 318
BsoBI CYCGRG 1 cut(s) 106
Bsp143I GATC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 2 cut(s) 182, 318
BssMI GATC 1 cut(s) 142
BssNI GRCGYC 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 288
BstACI GRCGYC 1 cut(s) 223
BstAFI CTTAAG 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 295
BstKTI GATC 1 cut(s) 145
BstMAI GTCTC 2 cut(s) 182, 318
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 78
BstPAI GACNNNNGTC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BtsCI GGATG 1 cut(s) 295
BveI ACCTGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 270
CviAII CATG 1 cut(s) 75
CviJI RGCY 3 cut(s) 194, 203, 303
CviKI_1 RGCY 3 cut(s) 194, 203, 303
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco31I GGTCTC 2 cut(s) 182, 318
Eco88I CYCGRG 1 cut(s) 106
FaeI CATG 1 cut(s) 78
FatI CATG 1 cut(s) 74
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 1 cut(s) 336
GluI GCNGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 223
Hin1II CATG 1 cut(s) 78
Hpy188III TCNNGA 3 cut(s) 98, 179, 283
HpyAV CCTTC 2 cut(s) 293, 303
HpyCH4IV ACGT 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 223
Hsp92I GRCGYC 1 cut(s) 223
Hsp92II CATG 1 cut(s) 78
Kzo9I GATC 1 cut(s) 142
LpnPI CCDG 4 cut(s) 58, 83, 252, 296
Lsp1109I GCAGC 1 cut(s) 181
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 1 cut(s) 27
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 4 cut(s) 124, 146, 194, 305
MflI RGATCY 1 cut(s) 142
MnlI CCTC 2 cut(s) 253, 289
MseI TTAA 2 cut(s) 210, 233
MspCI CTTAAG 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeII GATC 1 cut(s) 142
NlaIII CATG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 27
NspI RCATGY 1 cut(s) 78
PciI ACATGT 1 cut(s) 74
PkrI GCNGC 1 cut(s) 196
PscI ACATGT 1 cut(s) 74
PshAI GACNNNNGTC 1 cut(s) 77
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
SaqAI TTAA 2 cut(s) 210, 233
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 1 cut(s) 142
ScaI AGTACT 1 cut(s) 271
SetI ASST 6 cut(s) 47, 178, 196, 205, 226, 314
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SpeI ACTAGT 1 cut(s) 335
SspMI CTAG 1 cut(s) 336
TaiI ACGT 1 cut(s) 226
TaqI TCGA 1 cut(s) 180
TatI WGTACW 1 cut(s) 269
Tru1I TTAA 2 cut(s) 210, 233
Tru9I TTAA 2 cut(s) 210, 233
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 2 cut(s) 147, 306
TspGWI ACGGA 1 cut(s) 135
Vha464I CTTAAG 1 cut(s) 209
XceI RCATGY 1 cut(s) 78
XspI CTAG 1 cut(s) 336
ZraI GACGTC 1 cut(s) 224
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.