Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004178

Physical Location & Seq

Genomic Coordinates Reverse (-)

34544636 .. 34550060

Gene Structure 5425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1286100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGTTTCAGGACCAAACAAGCAATTGTAGGAGTTTTGGAAGCATTAATCATCCATCTAGTCAATCGAGAAAGATATCAATTGGAGTTGTGGTAGATCCATCAACCAAGAAAACGTCTGGAGTCACCAAGGAAGGTGAGGCTGTGGTGCCAAATGCAGAAAGGGTGACATATAACTTAGGCAATACCACAGAGGAAATAAGCAAGGAGCAAGAAGTCACAGCTGCCAAGAAAACAAAACAAGCTACGGATCCAGAGCAGGTGAATTCCCCATGTATTAGTACTGCATCATTCCACCAGAAAATGCATACTACAGATGCCGTTCTTCATGCAAAACAGTCTTCTGATTTGCCATCTGGTAGTGGGAAGGAGTACAAACAGGATGGAACAAGGAATGCACCTGTTACATGTTCTGTTCCGTTTTCCGCAAACCAGACATCGTTATTCCAGTCTGATGGTAAGAAGCAGAAGAACTTTGATGGAGTTAGCTACAGAAAGAGGAGAGTGAAGGATGGATCAGTGGAACTTCAGGATTTCACATTTGCGACTGCACAGGAAGTCGTAATGACGGATAAGGATACACTAGTTAATAAAACAGATGCAACGGATAAGGATGTGATAGTGGATAAAACAGATGCAACAGAAGGCAGGAGAACTGAAACTCTCAGAAAAAAGCTTTGGGAGATATTGGGAACTGTTTCTTCGCCTGATGGTCAGCATTCCAAATCTCAGCCTCCTGAGGTTGCTGATAATAATTTAAATCCACAGCCGGAATTTGATCAAATGGGTGCCACAGTTGTCAAGCCTAACAATAACTCTGATATACCAGAGCAAAAATATGATGAGAAGGGTGATGCATTTATCAAACCCCGACAGAATTCAGATACTATAGAAATGGAATCTGAAAGTCCTGATAATATTGTAAGGAGACCTGTGACTCGTTCTTTGAGCCAAAAGAGAGGTCCAACCAAAAAACAGCAGACAAGTGCAAAAAATGGTCCATCCTCTGTTTACAAACTGAAACATCAAGACAACAACATATTTTCCTTTGAACAAGAATGTTATGGGAAGCTTCATGGCTCTTCTGGTGGTGGATCCTCAATGTCTATGCGGAAGAAGAGCGAGGTCAAAAGTTTCAGAACTGAGTCGCATGGGATCTGCCTTCCTGAAAAGGGTAAACCAACCAAGACTCAGAAAGAGATTAATAGGAGTGAGCCACAATCATCTGCTAAGCAAACAAGTTCTGTAGGCAAGAAAATAGGAGATTTTCATGGTTACCTACATGAGAATAAAAGAGAATATCAGGAACTGGAAAAAAATATTCAAGAGCAGGAAGTCTACCAATCACCACTCCAATACAAGAAATTTAAGGCGGGTTTTTTCACTCCAGCAAATAAAGACCACGAGGAAGACAATGGCAATCCAAACTTGAGGAAAGCTGCCAATCCAGAAGAAGATGATCTAAGTCCCGAATTTGGAATTAAAACACCCGTGTCAACTTCTTCCCTTAGCTCAATCCCAAACTCAGACCAAATGGTAGATGCTAGTAGTCCTGCTGTAACAGAGAGGAGATTTAGTGTCGGAGATATTCGAAGTTTCAGTACTTTCTGGACTCCAAAACAAGATTGTTGTGATAATGAACAAACAAAATCTTTTGATTCTGTAGAGGAACTCCAGGATTCGCCATTGAGAAAAACAACACCTGACATGGAAGAAAATGCGGCAGAAGAAGGCCTTCTTTCGTCACCATCTGAAGAGGGGGACTTGAGGAGTTGTGGAGAAGGTTCTCCTATTATCCAAGGACATGACTGGACTGAAGAAGACAACTGGATAGAGGAACCTTCAGAGCATAATCAAGTGGATGGACTGGCTAGGGCTGTTGAATTGTTTGCATTGGAATTAGTAAAACTTAAAACCAAAATGAAGTCTGCAACTAATAGGAAATCCACTGAAATTTTGGTATCTGTTGCCGCGGAGGTACATATGCAGCTGCAAACCATTGAGTCTCAGATTCAAACAGACATAGGAAAGCATACAAACCTCAGTAAATCAAAGAAAAAAAGGCTGGAAACAAGGTTTGAAGAGCAACAAGAACAACTGAAAGTGATTTATGACAAGTTCAAGGAACAAGTCAATCAGCATCTTCAGGAATGCAAGAGCACACTTGAAGGGCTGGAAGTGTACCAGACAGAGTTTAAGGGAACTGTTGATAAGCAAAAAGCATCACACAGAAAGCTTCTCATGCGAGTGGAAGAAGCAATTGAGACCCAAATCAACGATGCCCAAAGAAAAATCCAAGTCACCCGAAGGATGGCGCGTGGAAAGATGCTCCAATTGAAACACGAACTGGCATTGTGTTTAAAAGACGGCATTCTAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0007049 GO:0008150 GO:0009987 GO:0010564 GO:0022414 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0040020 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044087 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051128 GO:0051321 GO:0051445 GO:0051726 GO:0051783 GO:0051983 GO:0060629 GO:0060631 GO:0065007 GO:0070013 GO:0090173 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

793

Amino Acids

88.72

Weight (kDa)

6.22

Isoelectric Point (pI)

59.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ASY3-like	PF20435	7 - 276	3e-20	Meiosis-specific protein ASY3-like
ASY3-like	PF20435	282 - 611	4.1e-22	Meiosis-specific protein ASY3-like
ASY3-like	PF20435	595 - 788	1.8e-49	Meiosis-specific protein ASY3-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46980 AT2G46980 AT2G46980 AT2G46980 AT2G46980 AT2G46980 AT5G20995
fragaria_vesca	FvH4_1g26160 FvH4_1g26160 FvH4_4g08230
malus_domestica	MD09G1291300.v1.1 MD17G1286100.v1.1
prunus_persica	Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1 Prupe.3G001600_v2.0.a1
pyrus_communis	pycom09g19920 pycom17g28230
rosa_chinensis	RchiOBHm_Chr3g0485651
rosa_laevigata	RLG00000023137
rosa_multiflora	Rmu_co8410931.1_g000001
rosa_roxburghii	Rroxscaffold_6G00396550
rosa_rugosa	Rorug03G0220500
rosa_samantha	Rh3AG269400 Rh3BG305200 Rh3CG303500 Rh3DG299700
rosa_wichuraiana	Rw3G024010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	247
Acc36I	ACCTGC	1 cut(s)	247
AccB1I	GGYRCC	2 cut(s)	145, 785
AccI	GTMKAC	1 cut(s)	1335
AccII	CGCG	2 cut(s)	1970, 2314
AciI	CCGC	6 cut(s)	423, 1108, 1370, 1718, 1968, 1970
AclWI	GGATC	7 cut(s)	89, 242, 255, 520, 1086, 1099, 1160
AcsI	RAATTY	6 cut(s)	262, 770, 874, 1361, 1469, 1950
AcuI	CTGAAG	5 cut(s)	509, 1770, 1824, 1833, 2126
AfaI	GTAC	5 cut(s)	280, 371, 1600, 1977, 2180
AfiI	CCNNNNNNNGG	3 cut(s)	1169, 1472, 2308
AflIII	ACRYGT	1 cut(s)	404
AgsI	TTSAA	8 cut(s)	1049, 1322, 1880, 2012, 2078, 2119, 2165, 2335
AhlI	ACTAGT	1 cut(s)	580
AjnI	CCWGG	1 cut(s)	1671
AjuI	GAANNNNNNNTTGG	2 cut(s)	658, 690
AluBI	AGCT	9 cut(s)	221, 242, 486, 673, 1069, 1436, 1509, 1987, 2233
AluI	AGCT	9 cut(s)	221, 242, 486, 673, 1069, 1436, 1509, 1987, 2233
Alw21I	GWGCWC	1 cut(s)	2159
Alw26I	GTCTC	3 cut(s)	919, 2007, 2255
AlwI	GGATC	7 cut(s)	89, 242, 255, 520, 1086, 1099, 1160
AlwNI	CAGNNNCTG	1 cut(s)	1306
AoxI	GGCC	1 cut(s)	1729
ApeKI	GCWGC	4 cut(s)	221, 1436, 1984, 1987
ApoI	RAATTY	6 cut(s)	262, 770, 874, 1361, 1469, 1950
ArsI	GACNNNNNNTTYG	4 cut(s)	925, 943, 957, 975
AseI	ATTAAT	2 cut(s)	45, 1200
Asp700I	GAANNNNTTC	2 cut(s)	694, 1731
AspLEI	GCGC	1 cut(s)	2314
AspS9I	GGNCC	3 cut(s)	10, 959, 995
AsuHPI	GGTGA	8 cut(s)	115, 146, 175, 271, 860, 1335, 1734, 2290
AsuII	TTCGAA	1 cut(s)	1588
AvaII	GGWCC	3 cut(s)	10, 959, 995
AxyI	CCTNAGG	1 cut(s)	735
BaeI	ACNNNNGTAYC	2 cut(s)	567, 600
BamHI	GGATCC	2 cut(s)	247, 1091
BanI	GGYRCC	2 cut(s)	145, 785
BarI	GAAGNNNNNNTAC	2 cut(s)	1582, 1614
BauI	CACGAG	1 cut(s)	1400
BbsI	GAAGAC	3 cut(s)	330, 1413, 1824
Bbv12I	GWGCWC	1 cut(s)	2159
BbvI	GCAGC	4 cut(s)	208, 1423, 1974, 1996
BceAI	ACGGC	1 cut(s)	302
BciT130I	CCWGG	1 cut(s)	1673
BciVI	GTATCC	1 cut(s)	568
BclI	TGATCA	1 cut(s)	775
BcoDI	GTCTC	3 cut(s)	919, 2007, 2255
BcuI	ACTAGT	1 cut(s)	580
BfaI	CTAG	4 cut(s)	57, 581, 1542, 1869
BfmI	CTRYAG	5 cut(s)	309, 487, 885, 1242, 1659
BfuAI	ACCTGC	1 cut(s)	247
BfuI	GTATCC	1 cut(s)	568
BisI	GCNGC	6 cut(s)	222, 1437, 1719, 1968, 1985, 1988
BlpI	GCTNAGC	1 cut(s)	1227
BlsI	GCNGC	6 cut(s)	223, 1438, 1720, 1969, 1986, 1989
BmcAI	AGTACT	2 cut(s)	280, 1600
Bme1390I	CCNGG	1 cut(s)	1673
Bme18I	GGWCC	3 cut(s)	10, 959, 995
BmgT120I	GGNCC	3 cut(s)	10, 959, 995
BmiI	GGNNCC	5 cut(s)	147, 249, 787, 1093, 1836
BmrFI	CCNGG	1 cut(s)	1673
BpiI	GAAGAC	3 cut(s)	330, 1413, 1824
BpmI	CTGGAG	3 cut(s)	138, 1368, 1655
Bpu10I	CCTNAGC	1 cut(s)	1505
Bpu1102I	GCTNAGC	1 cut(s)	1227
Bpu14I	TTCGAA	1 cut(s)	1588
BpuEI	CTTGAG	2 cut(s)	1447, 1783
BsaI	GGTCTC	2 cut(s)	919, 2255
BsaJI	CCNNGG	3 cut(s)	126, 1795, 1968
BsaXI	ACNNNNNCTCC	4 cut(s)	75, 105, 1558, 1588
Bsc4I	CCNNNNNNNGG	3 cut(s)	1169, 1472, 2308
Bse1I	ACTGG	6 cut(s)	445, 1311, 1811, 1829, 1869, 2349
Bse21I	CCTNAGG	1 cut(s)	735
BseBI	CCWGG	1 cut(s)	1673
BseDI	CCNNGG	3 cut(s)	126, 1795, 1968
BseGI	GGATG	7 cut(s)	49, 385, 514, 616, 998, 1864, 2313
BseLI	CCNNNNNNNGG	3 cut(s)	1169, 1472, 2308
BseMII	CTCAG	8 cut(s)	676, 726, 740, 1131, 1202, 1536, 2018, 2053
BseNI	ACTGG	6 cut(s)	445, 1311, 1811, 1829, 1869, 2349
BseRI	GAGGAG	3 cut(s)	511, 1579, 1780
BseXI	GCAGC	4 cut(s)	208, 1423, 1974, 1996
BsgI	GTGCAG	1 cut(s)	531
Bsh1236I	CGCG	2 cut(s)	1970, 2314
BshFI	GGCC	1 cut(s)	1731
BshNI	GGYRCC	2 cut(s)	145, 785
BsiHKAI	GWGCWC	1 cut(s)	2159
BsiSI	CCGG	1 cut(s)	767
BslFI	GGGAC	2 cut(s)	1449, 1772
BslI	CCNNNNNNNGG	3 cut(s)	1169, 1472, 2308
BsmAI	GTCTC	3 cut(s)	919, 2007, 2255
BsmFI	GGGAC	2 cut(s)	1449, 1772
BsmI	GAATGC	4 cut(s)	397, 715, 2153, 2367
BsnI	GGCC	1 cut(s)	1731
Bso31I	GGTCTC	2 cut(s)	919, 2255
Bsp119I	TTCGAA	1 cut(s)	1588
Bsp1286I	GDGCHC	1 cut(s)	2159
Bsp143I	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
Bsp1720I	GCTNAGC	1 cut(s)	1227
BspACI	CCGC	6 cut(s)	423, 1108, 1370, 1718, 1968, 1970
BspANI	GGCC	1 cut(s)	1731
BspCNI	CTCAG	8 cut(s)	675, 727, 739, 1132, 1201, 1535, 2017, 2052
BspFNI	CGCG	2 cut(s)	1970, 2314
BspLI	GGNNCC	5 cut(s)	147, 249, 787, 1093, 1836
BspMI	ACCTGC	1 cut(s)	247
BspPI	GGATC	7 cut(s)	89, 242, 255, 520, 1086, 1099, 1160
BspQI	GCTCTTC	3 cut(s)	1084, 1109, 2073
BspT104I	TTCGAA	1 cut(s)	1588
BspT107I	GGYRCC	2 cut(s)	145, 785
BspTNI	GGTCTC	2 cut(s)	919, 2255
BsrI	ACTGG	6 cut(s)	445, 1311, 1811, 1829, 1869, 2349
BssECI	CCNNGG	3 cut(s)	126, 1795, 1968
BssMI	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
BssSI	CACGAG	1 cut(s)	1400
BssT1I	CCWWGG	2 cut(s)	126, 1795
Bst2BI	CACGAG	1 cut(s)	1400
Bst2UI	CCWGG	1 cut(s)	1673
Bst4CI	ACNGT	4 cut(s)	336, 694, 793, 2203
Bst6I	CTCTTC	4 cut(s)	1084, 1109, 1746, 2073
BstBI	TTCGAA	1 cut(s)	1588
BstDSI	CCRYGG	1 cut(s)	1968
BstEII	GGTNACC	1 cut(s)	1271
BstF5I	GGATG	7 cut(s)	49, 385, 514, 616, 998, 1864, 2313
BstFNI	CGCG	2 cut(s)	1970, 2314
BstHHI	GCGC	1 cut(s)	2314
BstKTI	GATC	7 cut(s)	97, 250, 515, 778, 1094, 1155, 1459
BstMAI	GTCTC	3 cut(s)	919, 2007, 2255
BstMBI	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
BstMWI	GCNNNNNNNGC	1 cut(s)	2239
BstNI	CCWGG	1 cut(s)	1673
BstNSI	RCATGY	1 cut(s)	408
BstPI	GGTNACC	1 cut(s)	1271
BstSCI	CCNGG	1 cut(s)	1671
BstSFI	CTRYAG	5 cut(s)	309, 487, 885, 1242, 1659
BstUI	CGCG	2 cut(s)	1970, 2314
BstV1I	GCAGC	4 cut(s)	208, 1423, 1974, 1996
BstV2I	GAAGAC	3 cut(s)	330, 1413, 1824
BstX2I	RGATCY	4 cut(s)	94, 247, 1091, 1152
BstXI	CCANNNNNNTGG	1 cut(s)	452
BstYI	RGATCY	4 cut(s)	94, 247, 1091, 1152
Bsu36I	CCTNAGG	1 cut(s)	735
BsuI	GTATCC	1 cut(s)	568
BsuRI	GGCC	1 cut(s)	1731
BtgI	CCRYGG	1 cut(s)	1968
BtsCI	GGATG	7 cut(s)	49, 385, 514, 616, 998, 1864, 2313
BtsIMutI	CAGTG	2 cut(s)	522, 1944
BveI	ACCTGC	1 cut(s)	247
CaiI	CAGNNNCTG	1 cut(s)	1306
CfoI	GCGC	1 cut(s)	2314
Cfr13I	GGNCC	3 cut(s)	10, 959, 995
Cfr42I	CCGCGG	1 cut(s)	1971
Csp6I	GTAC	5 cut(s)	279, 370, 1599, 1976, 2179
CviQI	GTAC	5 cut(s)	279, 370, 1599, 1976, 2179
DpnI	GATC	7 cut(s)	96, 249, 514, 777, 1093, 1154, 1458
DpnII	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
DraI	TTTAAA	2 cut(s)	756, 2358
Eam1104I	CTCTTC	4 cut(s)	1084, 1109, 1746, 2073
EarI	CTCTTC	4 cut(s)	1084, 1109, 1746, 2073
Eco130I	CCWWGG	2 cut(s)	126, 1795
Eco147I	AGGCCT	1 cut(s)	1731
Eco31I	GGTCTC	2 cut(s)	919, 2255
Eco32I	GATATC	1 cut(s)	75
Eco47I	GGWCC	3 cut(s)	10, 959, 995
Eco57I	CTGAAG	5 cut(s)	509, 1770, 1824, 1833, 2126
Eco81I	CCTNAGG	1 cut(s)	735
Eco91I	GGTNACC	1 cut(s)	1271
EcoO65I	GGTNACC	1 cut(s)	1271
EcoRI	GAATTC	2 cut(s)	262, 874
EcoRII	CCWGG	1 cut(s)	1671
EcoRV	GATATC	1 cut(s)	75
EcoT14I	CCWWGG	2 cut(s)	126, 1795
EcoT22I	ATGCAT	2 cut(s)	306, 856
ErhI	CCWWGG	2 cut(s)	126, 1795
FalI	AAGNNNNNCTT	4 cut(s)	1716, 1748, 1719, 1751
FaqI	GGGAC	2 cut(s)	1449, 1772
FauI	CCCGC	1 cut(s)	1363
FauNDI	CATATG	1 cut(s)	1980
FbaI	TGATCA	1 cut(s)	775
FblI	GTMKAC	1 cut(s)	1335
Fnu4HI	GCNGC	6 cut(s)	222, 1437, 1719, 1968, 1985, 1988
FokI	GGATG	7 cut(s)	36, 392, 521, 623, 985, 1871, 2320
Fsp4HI	GCNGC	6 cut(s)	222, 1437, 1719, 1968, 1985, 1988
FspBI	CTAG	4 cut(s)	57, 581, 1542, 1869
GlaI	GCGC	1 cut(s)	2313
GluI	GCNGC	6 cut(s)	222, 1437, 1719, 1968, 1985, 1988
GsuI	CTGGAG	3 cut(s)	138, 1368, 1655
HaeIII	GGCC	1 cut(s)	1731
HapII	CCGG	1 cut(s)	767
HhaI	GCGC	1 cut(s)	2314
Hin6I	GCGC	1 cut(s)	2312
HinP1I	GCGC	1 cut(s)	2312
HincII	GTYRAC	1 cut(s)	1494
HindII	GTYRAC	1 cut(s)	1494
HindIII	AAGCTT	3 cut(s)	671, 1067, 2231
HpaII	CCGG	1 cut(s)	767
HphI	GGTGA	8 cut(s)	115, 146, 175, 271, 860, 1335, 1734, 2290
Hpy166II	GTNNAC	5 cut(s)	1009, 1175, 1336, 1494, 2179
Hpy8I	GTNNAC	5 cut(s)	1009, 1175, 1336, 1494, 2179
HpyCH4III	ACNGT	4 cut(s)	336, 694, 793, 2203
HpyCH4IV	ACGT	1 cut(s)	113
HpyF10VI	GCNNNNNNNGC	1 cut(s)	2239
HpySE526I	ACGT	1 cut(s)	113
HspAI	GCGC	1 cut(s)	2312
Ksp22I	TGATCA	1 cut(s)	775
KspI	CCGCGG	1 cut(s)	1971
Kzo9I	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
LguI	GCTCTTC	3 cut(s)	1084, 1109, 2073
LmnI	GCTCC	2 cut(s)	205, 2331
Lsp1109I	GCAGC	4 cut(s)	208, 1423, 1974, 1996
MaeI	CTAG	4 cut(s)	57, 581, 1542, 1869
MaeII	ACGT	1 cut(s)	113
MaeIII	GTNAC	9 cut(s)	121, 163, 214, 400, 931, 1271, 1555, 1740, 2296
MalI	GATC	7 cut(s)	96, 249, 514, 777, 1093, 1154, 1458
MboI	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
MfeI	CAATTG	4 cut(s)	22, 78, 2256, 2330
MflI	RGATCY	4 cut(s)	94, 247, 1091, 1152
MhlI	GDGCHC	1 cut(s)	2159
MlyI	GAGTC	6 cut(s)	129, 928, 1151, 1180, 1603, 2009
MmeI	TCCRAC	2 cut(s)	986, 1558
Mph1103I	ATGCAT	2 cut(s)	306, 856
MroXI	GAANNNNTTC	2 cut(s)	694, 1731
MseI	TTAA	9 cut(s)	45, 585, 755, 1200, 1365, 1479, 1908, 2193, 2357
MslI	CAYNNNNRTG	1 cut(s)	2243
MspA1I	CMGCKG	3 cut(s)	221, 1970, 1987
MspI	CCGG	1 cut(s)	767
MspR9I	CCNGG	1 cut(s)	1673
MunI	CAATTG	4 cut(s)	22, 78, 2256, 2330
Mva1269I	GAATGC	4 cut(s)	397, 715, 2153, 2367
MvaI	CCWGG	1 cut(s)	1673
MvnI	CGCG	2 cut(s)	1970, 2314
MwoI	GCNNNNNNNGC	1 cut(s)	2239
NdeI	CATATG	1 cut(s)	1980
NdeII	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
NlaIV	GGNNCC	5 cut(s)	147, 249, 787, 1093, 1836
NmuCI	GTSAC	6 cut(s)	121, 163, 214, 931, 1740, 2296
NsiI	ATGCAT	2 cut(s)	306, 856
NspI	RCATGY	1 cut(s)	408
NspV	TTCGAA	1 cut(s)	1588
PaqCI	CACCTGC	1 cut(s)	247
PceI	AGGCCT	1 cut(s)	1731
PciI	ACATGT	1 cut(s)	404
PciSI	GCTCTTC	3 cut(s)	1084, 1109, 2073
PctI	GAATGC	4 cut(s)	397, 715, 2153, 2367
PdmI	GAANNNNTTC	2 cut(s)	694, 1731
PfeI	GAWTC	4 cut(s)	896, 1655, 1676, 2008
PfoI	TCCNGGA	1 cut(s)	1671
PkrI	GCNGC	6 cut(s)	223, 1438, 1720, 1969, 1986, 1989
PleI	GAGTC	6 cut(s)	128, 928, 1150, 1180, 1603, 2008
PpsI	GAGTC	6 cut(s)	128, 928, 1150, 1180, 1603, 2008
PscI	ACATGT	1 cut(s)	404
PshBI	ATTAAT	2 cut(s)	45, 1200
Psp6I	CCWGG	1 cut(s)	1671
PspEI	GGTNACC	1 cut(s)	1271
PspGI	CCWGG	1 cut(s)	1671
PspN4I	GGNNCC	5 cut(s)	147, 249, 787, 1093, 1836
PspPI	GGNCC	3 cut(s)	10, 959, 995
PstNI	CAGNNNCTG	1 cut(s)	1306
PsuI	RGATCY	4 cut(s)	94, 247, 1091, 1152
PvuII	CAGCTG	2 cut(s)	221, 1987
RsaI	GTAC	5 cut(s)	280, 371, 1600, 1977, 2180
RsaNI	GTAC	5 cut(s)	279, 370, 1599, 1976, 2179
RseI	CAYNNNNRTG	1 cut(s)	2243
SacII	CCGCGG	1 cut(s)	1971
SapI	GCTCTTC	3 cut(s)	1084, 1109, 2073
SaqAI	TTAA	9 cut(s)	45, 585, 755, 1200, 1365, 1479, 1908, 2193, 2357
SatI	GCNGC	6 cut(s)	222, 1437, 1719, 1968, 1985, 1988
Sau3AI	GATC	7 cut(s)	94, 247, 512, 775, 1091, 1152, 1456
Sau96I	GGNCC	3 cut(s)	10, 959, 995
ScaI	AGTACT	2 cut(s)	280, 1600
SchI	GAGTC	6 cut(s)	129, 928, 1151, 1180, 1603, 2009
ScrFI	CCNGG	1 cut(s)	1673
SduI	GDGCHC	1 cut(s)	2159
SfcI	CTRYAG	5 cut(s)	309, 487, 885, 1242, 1659
Sfr303I	CCGCGG	1 cut(s)	1971
SfuI	TTCGAA	1 cut(s)	1588
SgrBI	CCGCGG	1 cut(s)	1971
SinI	GGWCC	3 cut(s)	10, 959, 995
SmiI	ATTTAAAT	1 cut(s)	756
SmiMI	CAYNNNNRTG	1 cut(s)	2243
SmlI	CTYRAG	2 cut(s)	1426, 1762
SmoI	CTYRAG	2 cut(s)	1426, 1762
SpeI	ACTAGT	1 cut(s)	580
SseBI	AGGCCT	1 cut(s)	1731
SsiI	CCGC	6 cut(s)	423, 1108, 1370, 1718, 1968, 1970
SspI	AATATT	2 cut(s)	916, 1318
SspMI	CTAG	4 cut(s)	57, 581, 1542, 1869
StuI	AGGCCT	1 cut(s)	1731
StyD4I	CCNGG	1 cut(s)	1671
StyI	CCWWGG	2 cut(s)	126, 1795
SwaI	ATTTAAAT	1 cut(s)	756
TaaI	ACNGT	4 cut(s)	336, 694, 793, 2203
TaiI	ACGT	1 cut(s)	116
TaqI	TCGA	2 cut(s)	65, 1588
TatI	WGTACW	3 cut(s)	278, 369, 1598
TauI	GCSGC	2 cut(s)	1721, 1970
TfiI	GAWTC	4 cut(s)	896, 1655, 1676, 2008
Tru1I	TTAA	9 cut(s)	45, 585, 755, 1200, 1365, 1479, 1908, 2193, 2357
Tru9I	TTAA	9 cut(s)	45, 585, 755, 1200, 1365, 1479, 1908, 2193, 2357
TscAI	CASTG	2 cut(s)	522, 1951
TseFI	GTSAC	6 cut(s)	121, 163, 214, 931, 1740, 2296
TseI	GCWGC	4 cut(s)	221, 1436, 1984, 1987
Tsp45I	GTSAC	6 cut(s)	121, 163, 214, 931, 1740, 2296
TspDTI	ATGAA	5 cut(s)	314, 1061, 1256, 1650, 1934
TspGWI	ACGGA	4 cut(s)	260, 405, 581, 617
TspRI	CASTG	2 cut(s)	522, 1951
VpaK11BI	GGWCC	3 cut(s)	10, 959, 995
VspI	ATTAAT	2 cut(s)	45, 1200
XapI	RAATTY	6 cut(s)	262, 770, 874, 1361, 1469, 1950
XceI	RCATGY	1 cut(s)	408
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1951
XmiI	GTMKAC	1 cut(s)	1335
XmnI	GAANNNNTTC	2 cut(s)	694, 1731
XspI	CTAG	4 cut(s)	57, 581, 1542, 1869
ZrmI	AGTACT	2 cut(s)	280, 1600
Zsp2I	ATGCAT	2 cut(s)	306, 856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.