AT5G23220

nicotinamidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016656
Physical Location & Seq
Reverse (-)
7819608 .. 7820760
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23220.1

Sequence Viewer

Length: 597 bp
ATGGCTTCCTCATCAACGAGAAAGTACGAGACACGAAAGCGAGATCCAAACTCTAAAATCGCAGCTCTTCTCGTTATCGACATGCAGAATCACTTCTCCTCCATGGCCAAACCCATCCTCAACAACGTTCTCACCACCATCGACATCTGCCGACGCGCCTCAGTCCCCGTATTCTTTACGCGTCACAACCACAAATCCCCGACCGACCACGGCATGCTCGGCGAGTGGTGTAACGGCGATGTAATCCTTGACGGAACCACCGATTCTGAAATCATCCAGGAGATACAAGGCCAAGTAACCGGACCAGACGAGATGGTGGAGAAGAACACGTACAGTGCGTTTAACAAAACCCGCCTCCAGGAAAACCTGGAAAAGATCGGAGTAAAGGAGGTGATCGTGATCGGAGTGATGACGAACTTGTGCTGTGAGACAACGGCGCGTGAAGCGTTTATTAAGGGTTTTAGGGTTTTTTTCTCGACGGACGCGACTGCGACGTTTAATGAGGAGCTTCACGAGGCTACGCTAATGAATCTCGCTTTTGGCTTCGCTTATCTCGTCGATTGCGATAAACTCCGGCGAAGTCTACTCGGTAACTAA

Protein Analysis

198

Amino Acids

22.23

Weight (kDa)

5.97

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 21 - 187 4e-42 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 583
AccII CGCG 4 cut(s) 156, 181, 439, 485
AciI CCGC 1 cut(s) 352
AclI AACGTT 1 cut(s) 126
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 105
AfaI GTAC 2 cut(s) 26, 332
AfiI CCNNNNNNNGG 1 cut(s) 358
AflIII ACRYGT 2 cut(s) 179, 327
AjnI CCWGG 3 cut(s) 276, 357, 366
AluBI AGCT 2 cut(s) 65, 508
AluI AGCT 2 cut(s) 65, 508
Alw26I GTCTC 2 cut(s) 23, 422
AlwI GGATC 1 cut(s) 38
AoxI GGCC 2 cut(s) 105, 289
ApeKI GCWGC 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 158, 439
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 124, 403
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BauI CACGAG 1 cut(s) 512
BbvI GCAGC 1 cut(s) 74
BccI CCATC 3 cut(s) 122, 146, 307
BceAI ACGGC 3 cut(s) 226, 250, 450
BciT130I CCWGG 3 cut(s) 278, 359, 368
BcoDI GTCTC 2 cut(s) 23, 422
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 3 cut(s) 278, 359, 368
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 3 cut(s) 278, 359, 368
BpmI CTGGAG 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 102, 208
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 3 cut(s) 278, 359, 368
BseDI CCNNGG 2 cut(s) 102, 208
BseGI GGATG 2 cut(s) 114, 273
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 88, 518
BseXI GCAGC 1 cut(s) 74
Bsh1236I CGCG 4 cut(s) 156, 181, 439, 485
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 107, 291
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 300, 574
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 358
BsmAI GTCTC 2 cut(s) 23, 422
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 107, 291
Bsp143I GATC 4 cut(s) 43, 375, 393, 399
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 1 cut(s) 352
BspANI GGCC 2 cut(s) 107, 291
BspCNI CTCAG 1 cut(s) 173
BspFNI CGCG 4 cut(s) 156, 181, 439, 485
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 38
BspQI GCTCTTC 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 102, 208
BssMI GATC 4 cut(s) 43, 375, 393, 399
BssSI CACGAG 1 cut(s) 512
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 512
Bst2UI CCWGG 3 cut(s) 278, 359, 368
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 2 cut(s) 102, 208
BstF5I GGATG 2 cut(s) 114, 273
BstFNI CGCG 4 cut(s) 156, 181, 439, 485
BstHHI GCGC 2 cut(s) 158, 439
BstKTI GATC 4 cut(s) 46, 378, 396, 402
BstMAI GTCTC 2 cut(s) 23, 422
BstMBI GATC 4 cut(s) 43, 375, 393, 399
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 219, 443
BstNI CCWGG 3 cut(s) 278, 359, 368
BstNSI RCATGY 2 cut(s) 85, 217
BstSCI CCNGG 3 cut(s) 276, 357, 366
BstUI CGCG 4 cut(s) 156, 181, 439, 485
BstV1I GCAGC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 2 cut(s) 107, 291
BtgI CCRYGG 2 cut(s) 102, 208
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 114, 273
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 2 cut(s) 158, 439
Cfr13I GGNCC 1 cut(s) 302
CseI GACGC 3 cut(s) 162, 170, 491
Csp6I GTAC 2 cut(s) 25, 331
CviAII CATG 3 cut(s) 82, 103, 214
CviJI RGCY 7 cut(s) 5, 65, 107, 291, 508, 518, 543
CviKI_1 RGCY 7 cut(s) 5, 65, 107, 291, 508, 518, 543
CviQI GTAC 2 cut(s) 25, 331
DdeI CTNAG 1 cut(s) 160
DpnI GATC 4 cut(s) 45, 377, 395, 401
DpnII GATC 4 cut(s) 43, 375, 393, 399
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 302
EcoRII CCWGG 3 cut(s) 276, 357, 366
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 3 cut(s) 85, 106, 217
FaiI YATR 3 cut(s) 83, 104, 215
FaqI GGGAC 1 cut(s) 149
FatI CATG 3 cut(s) 81, 102, 213
FauI CCCGC 1 cut(s) 359
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 2 cut(s) 101, 260
Fsp4HI GCNGC 1 cut(s) 63
GlaI GCGC 2 cut(s) 157, 438
GluI GCNGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 2 cut(s) 107, 291
HapII CCGG 2 cut(s) 300, 574
HgaI GACGC 3 cut(s) 162, 170, 491
HhaI GCGC 2 cut(s) 158, 439
Hin1II CATG 3 cut(s) 85, 106, 217
Hin6I GCGC 2 cut(s) 156, 437
HinP1I GCGC 2 cut(s) 156, 437
HinfI GANTC 3 cut(s) 88, 263, 529
HpaII CCGG 2 cut(s) 300, 574
HphI GGTGA 2 cut(s) 124, 403
Hpy166II GTNNAC 1 cut(s) 584
Hpy188I TCNGA 3 cut(s) 268, 380, 404
Hpy188III TCNNGA 3 cut(s) 397, 475, 512
Hpy8I GTNNAC 1 cut(s) 584
Hpy99I CGWCG 4 cut(s) 156, 481, 496, 560
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4IV ACGT 3 cut(s) 126, 329, 494
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 443
HpyF3I CTNAG 1 cut(s) 160
HpySE526I ACGT 3 cut(s) 126, 329, 494
Hsp92II CATG 3 cut(s) 85, 106, 217
HspAI GCGC 2 cut(s) 156, 437
Kzo9I GATC 4 cut(s) 43, 375, 393, 399
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 1 cut(s) 505
LpnPI CCDG 9 cut(s) 263, 290, 313, 318, 344, 353, 371, 380, 587
Lsp1109I GCAGC 1 cut(s) 74
MaeII ACGT 3 cut(s) 126, 329, 494
MaeIII GTNAC 4 cut(s) 182, 230, 295, 590
MalI GATC 4 cut(s) 45, 377, 395, 401
MboI GATC 4 cut(s) 43, 375, 393, 399
MboII GAAGA 2 cut(s) 59, 334
MflI RGATCY 1 cut(s) 43
MlsI TGGCCA 1 cut(s) 107
MluI ACGCGT 1 cut(s) 179
MluNI TGGCCA 1 cut(s) 107
MnlI CCTC 8 cut(s) 19, 109, 128, 169, 365, 382, 496, 508
Mox20I TGGCCA 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 92
MscI TGGCCA 1 cut(s) 107
MseI TTAA 3 cut(s) 342, 453, 498
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 2 cut(s) 300, 574
MspR9I CCNGG 3 cut(s) 278, 359, 368
MvaI CCWGG 3 cut(s) 278, 359, 368
MvnI CGCG 4 cut(s) 156, 181, 439, 485
MwoI GCNNNNNNNGC 2 cut(s) 219, 443
NcoI CCATGG 1 cut(s) 102
NdeII GATC 4 cut(s) 43, 375, 393, 399
NlaIII CATG 3 cut(s) 85, 106, 217
NlaIV GGNNCC 1 cut(s) 256
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 182
NspI RCATGY 2 cut(s) 85, 217
PaeI GCATGC 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 5 cut(s) 258, 335, 482, 552, 561
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 3 cut(s) 88, 263, 529
PfoI TCCNGGA 2 cut(s) 276, 357
PkrI GCNGC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 3 cut(s) 276, 357, 366
PspGI CCWGG 3 cut(s) 276, 357, 366
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 2 cut(s) 26, 332
RsaNI GTAC 2 cut(s) 25, 331
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 342, 453, 498
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 4 cut(s) 43, 375, 393, 399
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 3 cut(s) 278, 359, 368
SetI ASST 7 cut(s) 67, 129, 332, 369, 393, 497, 510
SinI GGWCC 1 cut(s) 302
SphI GCATGC 1 cut(s) 217
SsiI CCGC 1 cut(s) 352
StyD4I CCNGG 3 cut(s) 276, 357, 366
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 335
TaiI ACGT 3 cut(s) 129, 332, 497
TaqI TCGA 4 cut(s) 78, 141, 476, 558
TaqII GACCGA 1 cut(s) 218
TfiI GAWTC 3 cut(s) 88, 263, 529
Tru1I TTAA 3 cut(s) 342, 453, 498
Tru9I TTAA 3 cut(s) 342, 453, 498
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 542
TspGWI ACGGA 2 cut(s) 267, 494
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 302
XceI RCATGY 2 cut(s) 85, 217
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.