Rh4DG444200

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016656
Physical Location & Seq
Reverse (-)
64480615 .. 64481211
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG444200.1

Sequence Viewer

Length: 597 bp
ATGGCTATGGCTTCCTCCTCGACCTCGTACAAAGAGTACGAGATCAGAAAAAGAAACCCAAACCCGAAAGTGGCTGCCCTTTTAGTCATCGACATGCAGAACTACTTCGCCGCCATGGCTAAGCCTATCCTCCAAAACCTCCTCACCACCATCCGTCTCTGCCGACGCGCTTCCATCCCCGTCATCTTCACGCGCCACTCCCACAAGTCCCCCTCCGACTATGGCATGCTGGACGAGTGGTGGAACGGCGACCTCGTCTTCGACGGGACTCCCGAGTCCCAGCTGATGCCCGAGCTCGACCGCGGGCCCCACCAGGACCACGTCGTCGACAAGAACACCTACAGCGCCTTCCGCGGCACGGGCCTCGAGGACTATTTGAAGGAGAGGGGCGTCCAGGAGGTCATCGTCACCGGCGTCATGACCAACTTGTGCTGCGAAACGACGGCGCGTGAGGCCTTCCTCCGGGGCTTCAGAGTGTTCTTTTCTACGGACGCCACTGCCACTTCGGATGAAGAGCTGCACGAGGCCACCTTGAAGAACTTGGCCTATGGCTTTGCCTACCTCGTCGATTGCAACAGACTTGATCAAGCTCTTTAG

Protein Analysis

198

Amino Acids

22.43

Weight (kDa)

5.55

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 25 - 190 1.3e-48 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 274, 323
AbsI CCTCGAGG 1 cut(s) 365
AccI GTMKAC 1 cut(s) 327
AccII CGCG 5 cut(s) 168, 193, 303, 354, 448
AciI CCGC 5 cut(s) 111, 301, 303, 352, 354
AcuI CTGAAG 1 cut(s) 454
AcyI GRCGYC 3 cut(s) 390, 414, 492
AfaI GTAC 2 cut(s) 29, 38
AfiI CCNNNNNNNGG 3 cut(s) 70, 358, 462
AgsI TTSAA 2 cut(s) 379, 535
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 312, 393
AluBI AGCT 4 cut(s) 283, 295, 517, 590
AluI AGCT 4 cut(s) 283, 295, 517, 590
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 1 cut(s) 161
Ama87I CYCGRG 3 cut(s) 272, 290, 365
AoxI GGCC 5 cut(s) 305, 361, 453, 525, 543
ApaI GGGCCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 74, 432, 517
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 1 cut(s) 104
AspLEI GCGC 4 cut(s) 170, 195, 347, 448
AspS9I GGNCC 4 cut(s) 305, 306, 316, 361
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 136, 400
AvaI CYCGRG 3 cut(s) 272, 290, 365
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 309
BanII GRGCYC 2 cut(s) 297, 309
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 61, 419, 504
BccI CCATC 2 cut(s) 158, 182
BceAI ACGGC 2 cut(s) 262, 459
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 2 cut(s) 314, 395
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 5 cut(s) 75, 111, 355, 433, 518
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 5 cut(s) 76, 112, 356, 434, 519
Bme1390I CCNGG 3 cut(s) 314, 395, 464
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 3 cut(s) 272, 290, 365
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 4 cut(s) 305, 306, 316, 361
BmiI GGNNCC 2 cut(s) 307, 308
BmrFI CCNGG 3 cut(s) 314, 395, 464
BmsI GCATC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 464
BsaHI GRCGYC 3 cut(s) 390, 414, 492
BsaJI CCNNGG 4 cut(s) 114, 301, 352, 463
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 358, 462
Bse118I RCCGGY 1 cut(s) 410
BseBI CCWGG 2 cut(s) 314, 395
BseDI CCNNGG 4 cut(s) 114, 301, 352, 463
BseGI GGATG 3 cut(s) 150, 174, 514
BseLI CCNNNNNNNGG 3 cut(s) 70, 358, 462
BseRI GAGGAG 2 cut(s) 7, 131
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 3 cut(s) 61, 419, 504
BseYI CCCAGC 1 cut(s) 279
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 5 cut(s) 168, 193, 303, 354, 448
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 5 cut(s) 307, 363, 455, 527, 545
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 3 cut(s) 272, 290, 365
BsiSI CCGG 2 cut(s) 411, 463
BslFI GGGAC 3 cut(s) 193, 262, 280
BslI CCNNNNNNNGG 3 cut(s) 70, 358, 462
BsmAI GTCTC 1 cut(s) 161
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 3 cut(s) 193, 262, 280
BsnI GGCC 5 cut(s) 307, 363, 455, 527, 545
BsoBI CYCGRG 3 cut(s) 272, 290, 365
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 297, 309
Bsp143I GATC 2 cut(s) 42, 583
Bsp1720I GCTNAGC 1 cut(s) 120
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 5 cut(s) 111, 301, 303, 352, 354
BspANI GGCC 5 cut(s) 307, 363, 455, 527, 545
BspFNI CGCG 5 cut(s) 168, 193, 303, 354, 448
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 307, 308
BspQI GCTCTTC 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 4 cut(s) 114, 301, 352, 463
BssMI GATC 2 cut(s) 42, 583
BssNI GRCGYC 3 cut(s) 390, 414, 492
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 114
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 314, 395
Bst6I CTCTTC 1 cut(s) 507
BstACI GRCGYC 3 cut(s) 390, 414, 492
BstC8I GCNNGC 2 cut(s) 227, 305
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 3 cut(s) 114, 301, 352
BstF5I GGATG 3 cut(s) 150, 174, 514
BstFNI CGCG 5 cut(s) 168, 193, 303, 354, 448
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 4 cut(s) 170, 195, 347, 448
BstKTI GATC 2 cut(s) 45, 586
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 42, 583
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 4 cut(s) 116, 351, 360, 452
BstNI CCWGG 2 cut(s) 314, 395
BstNSI RCATGY 2 cut(s) 97, 229
BstSCI CCNGG 3 cut(s) 312, 393, 462
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 309
BstUI CGCG 5 cut(s) 168, 193, 303, 354, 448
BstV1I GCAGC 3 cut(s) 61, 419, 504
BstV2I GAAGAC 1 cut(s) 250
BsuRI GGCC 5 cut(s) 307, 363, 455, 527, 545
BtgI CCRYGG 3 cut(s) 114, 301, 352
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 3 cut(s) 150, 174, 514
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 495
Cac8I GCNNGC 2 cut(s) 227, 305
CciI TCATGA 1 cut(s) 417
CfoI GCGC 4 cut(s) 170, 195, 347, 448
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 4 cut(s) 305, 306, 316, 361
Cfr42I CCGCGG 2 cut(s) 304, 355
CseI GACGC 4 cut(s) 174, 379, 403, 500
Csp6I GTAC 2 cut(s) 28, 37
CviAII CATG 4 cut(s) 94, 115, 226, 418
CviQI GTAC 2 cut(s) 28, 37
DdeI CTNAG 1 cut(s) 120
DpnI GATC 2 cut(s) 44, 585
DpnII GATC 2 cut(s) 42, 583
DrdI GACNNNNNNGTC 2 cut(s) 274, 323
DseDI GACNNNNNNGTC 2 cut(s) 274, 323
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 2 cut(s) 297, 309
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 454
Eco88I CYCGRG 3 cut(s) 272, 290, 365
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 312, 393
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 2 cut(s) 297, 309
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 4 cut(s) 97, 118, 229, 421
FaiI YATR 7 cut(s) 8, 95, 116, 222, 227, 419, 549
FaqI GGGAC 3 cut(s) 193, 262, 280
FatI CATG 4 cut(s) 93, 114, 225, 417
FauI CCCGC 1 cut(s) 296
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 5 cut(s) 75, 111, 355, 433, 518
FokI GGATG 3 cut(s) 137, 161, 521
FriOI GRGCYC 2 cut(s) 297, 309
Fsp4HI GCNGC 5 cut(s) 75, 111, 355, 433, 518
GlaI GCGC 4 cut(s) 169, 194, 346, 447
GluI GCNGC 5 cut(s) 75, 111, 355, 433, 518
GsaI CCCAGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 5 cut(s) 307, 363, 455, 527, 545
HapII CCGG 2 cut(s) 411, 463
HgaI GACGC 4 cut(s) 174, 379, 403, 500
HhaI GCGC 4 cut(s) 170, 195, 347, 448
Hin1I GRCGYC 3 cut(s) 390, 414, 492
Hin1II CATG 4 cut(s) 97, 118, 229, 421
Hin6I GCGC 4 cut(s) 168, 193, 345, 446
HinP1I GCGC 4 cut(s) 168, 193, 345, 446
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 2 cut(s) 268, 275
HpaII CCGG 2 cut(s) 411, 463
HphI GGTGA 2 cut(s) 136, 400
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 4 cut(s) 47, 217, 473, 508
Hpy188III TCNNGA 2 cut(s) 272, 418
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 6 cut(s) 168, 266, 326, 329, 445, 569
HpyAV CCTTC 3 cut(s) 358, 373, 466
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 97, 520, 573
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 351, 360, 452
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 3 cut(s) 390, 414, 492
Hsp92II CATG 4 cut(s) 97, 118, 229, 421
HspAI GCGC 4 cut(s) 168, 193, 345, 446
Ksp22I TGATCA 1 cut(s) 583
KspI CCGCGG 2 cut(s) 304, 355
Kzo9I GATC 2 cut(s) 42, 583
LguI GCTCTTC 1 cut(s) 507
LpnPI CCDG 8 cut(s) 215, 293, 299, 326, 380, 407, 424, 476
Lsp1109I GCAGC 3 cut(s) 61, 419, 504
LweI GCATC 1 cut(s) 276
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 406
MalI GATC 2 cut(s) 44, 585
MboI GATC 2 cut(s) 42, 583
MboII GAAGA 4 cut(s) 178, 250, 524, 547
MhlI GDGCHC 2 cut(s) 297, 309
MlyI GAGTC 2 cut(s) 262, 284
MmeI TCCRAC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 92
MspA1I CMGCKG 3 cut(s) 283, 303, 354
MspI CCGG 2 cut(s) 411, 463
MspR9I CCNGG 3 cut(s) 314, 395, 464
MvaI CCWGG 2 cut(s) 314, 395
MvnI CGCG 5 cut(s) 168, 193, 303, 354, 448
MwoI GCNNNNNNNGC 4 cut(s) 116, 351, 360, 452
NciI CCSGG 1 cut(s) 464
NcoI CCATGG 1 cut(s) 114
NdeII GATC 2 cut(s) 42, 583
NlaIII CATG 4 cut(s) 97, 118, 229, 421
NlaIV GGNNCC 2 cut(s) 307, 308
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 2 cut(s) 97, 229
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 365
PagI TCATGA 1 cut(s) 417
PceI AGGCCT 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 270, 411
PdmI GAANNNNTTC 1 cut(s) 104
PflFI GACNNNGTC 2 cut(s) 254, 320
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 5 cut(s) 76, 112, 356, 434, 519
PleI GAGTC 2 cut(s) 262, 283
PpsI GAGTC 2 cut(s) 262, 283
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 312, 393
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 2 cut(s) 312, 393
PspN4I GGNNCC 2 cut(s) 307, 308
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 4 cut(s) 305, 306, 316, 361
PspXI VCTCGAGB 1 cut(s) 365
PsyI GACNNNGTC 2 cut(s) 254, 320
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 2 cut(s) 29, 38
RsaNI GTAC 2 cut(s) 28, 37
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 297
SacII CCGCGG 2 cut(s) 304, 355
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 507
SatI GCNGC 5 cut(s) 75, 111, 355, 433, 518
Sau3AI GATC 2 cut(s) 42, 583
Sau96I GGNCC 4 cut(s) 305, 306, 316, 361
SchI GAGTC 2 cut(s) 262, 284
ScrFI CCNGG 3 cut(s) 314, 395, 464
SduI GDGCHC 2 cut(s) 297, 309
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 340
Sfr274I CTCGAG 1 cut(s) 365
Sfr303I CCGCGG 2 cut(s) 304, 355
SgrAI CRCCGGYG 1 cut(s) 410
SgrBI CCGCGG 2 cut(s) 304, 355
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SphI GCATGC 1 cut(s) 229
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 5 cut(s) 111, 301, 303, 352, 354
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 312, 393, 462
StyI CCWWGG 1 cut(s) 114
TaiI ACGT 1 cut(s) 324
TaqI TCGA 7 cut(s) 20, 90, 261, 297, 327, 366, 567
TauI GCSGC 2 cut(s) 113, 357
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 3 cut(s) 74, 432, 517
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 2 cut(s) 143, 503
TspRI CASTG 1 cut(s) 502
Tth111I GACNNNGTC 2 cut(s) 254, 320
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 2 cut(s) 97, 229
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.