MD16G1003500.v1.1

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016656
Physical Location & Seq
Reverse (-)
213658 .. 214284
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1003500.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCCTCCGCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCATACAGAAAATACGAGATCAGGAAACGAAACCCGAACCCAAAAGCGGCGGCCCTTCTAGTCATCGACGTGCAGAACTACTTCTCCGCCATGGCCCGACCCATCCTTCAAAACCTCCTCACCACCATCCGCCTCTGCCGACGGGCTTCCATTCCCGTCATCTTCACGCGCCACGCCCACAAGTCCCCCTCCGACTACGGCATGCTGGACGAGTGGTGGAACGGCGATCTCGTCTACGACGGCACTCCCGAATCGCAGCTCATGCCGGAGCTCGACCGGAGGGGACCCGACGAGGACCGCGTGTTCGACAAGAACACGTACAGCGCGTTCAGGAACACGGGCCTCGAGGAATACCTGAAGGAGAAGGGGGTGGAGGAGGTTATCGTAACCGGCGTCATGACCAACCTGTGCTGCGAAACGACGGCGCGTGAAGCGTTCATAAGGGGGTATAGAGTGTTTTTCTCCACGGACGCGACTGCGACGTCGGATCGGGAGCTGCACGAGGCGACCTTGAAAAACATGGCTTATGGGTTTGCCTACTTGGTGGATTGCAAGAGGCTTGAGGACGGCCTTTTTCCGGGGACATAA

Protein Analysis

209

Amino Acids

23.52

Weight (kDa)

5.98

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 30 - 196 1e-46 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 520
AatII GACGTC 1 cut(s) 524
AbsI CCTCGAGG 1 cut(s) 383
AccI GTMKAC 1 cut(s) 273
AccII CGCG 5 cut(s) 208, 339, 365, 466, 512
AciI CCGC 6 cut(s) 9, 86, 89, 126, 169, 337
AclWI GGATC 1 cut(s) 534
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 2 cut(s) 432, 521
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 85, 616
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 149, 553
AjiI CACGTC 1 cut(s) 109
AloI GAACNNNNNNTCC 4 cut(s) 107, 139, 326, 358
AluBI AGCT 3 cut(s) 298, 310, 535
AluI AGCT 3 cut(s) 298, 310, 535
Alw21I GWGCWC 1 cut(s) 312
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 5 cut(s) 3, 90, 132, 379, 607
ApeKI GCWGC 3 cut(s) 295, 450, 535
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 3 cut(s) 210, 365, 466
AspS9I GGNCC 5 cut(s) 91, 133, 323, 334, 379
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 2 cut(s) 323, 334
BanII GRGCYC 1 cut(s) 312
BauI CACGAG 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 307, 437, 522
BccI CCATC 2 cut(s) 149, 173
BceAI ACGGC 5 cut(s) 253, 277, 295, 477, 622
BcnI CCSGG 1 cut(s) 618
BfaI CTAG 1 cut(s) 98
BisI GCNGC 5 cut(s) 87, 90, 296, 451, 536
BlsI GCNGC 5 cut(s) 88, 91, 297, 452, 537
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 2 cut(s) 323, 334
BmeT110I CYCGRG 1 cut(s) 383
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 5 cut(s) 91, 133, 323, 334, 379
BmiI GGNNCC 2 cut(s) 324, 325
BmrFI CCNGG 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 357
BsaHI GRCGYC 2 cut(s) 432, 521
BsaJI CCNNGG 3 cut(s) 129, 504, 617
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 616
Bse118I RCCGGY 1 cut(s) 428
BseDI CCNNGG 3 cut(s) 129, 504, 617
BseGI GGATG 2 cut(s) 141, 165
BseLI CCNNNNNNNGG 2 cut(s) 85, 616
BseXI GCAGC 3 cut(s) 307, 437, 522
BsgI GTGCAG 2 cut(s) 131, 521
Bsh1236I CGCG 5 cut(s) 208, 339, 365, 466, 512
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 5, 92, 134, 381, 609
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 4 cut(s) 305, 316, 429, 617
BslFI GGGAC 2 cut(s) 208, 336
BslI CCNNNNNNNGG 2 cut(s) 85, 616
BsmFI GGGAC 2 cut(s) 208, 336
BsnI GGCC 5 cut(s) 5, 92, 134, 381, 609
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 3 cut(s) 57, 265, 526
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 6 cut(s) 9, 86, 89, 126, 169, 337
BspANI GGCC 5 cut(s) 5, 92, 134, 381, 609
BspFNI CGCG 5 cut(s) 208, 339, 365, 466, 512
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 324, 325
BspPI GGATC 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 428
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 129, 504, 617
BssMI GATC 3 cut(s) 57, 265, 526
BssNI GRCGYC 2 cut(s) 432, 521
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 539
BstACI GRCGYC 2 cut(s) 432, 521
BstAPI GCANNNNNTGC 1 cut(s) 301
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 242
BstDSI CCRYGG 2 cut(s) 129, 504
BstF5I GGATG 2 cut(s) 141, 165
BstFNI CGCG 5 cut(s) 208, 339, 365, 466, 512
BstHHI GCGC 3 cut(s) 210, 365, 466
BstKTI GATC 3 cut(s) 60, 268, 529
BstMBI GATC 3 cut(s) 57, 265, 526
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 2 cut(s) 301, 470
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 616
BstUI CGCG 5 cut(s) 208, 339, 365, 466, 512
BstV1I GCAGC 3 cut(s) 307, 437, 522
BsuRI GGCC 5 cut(s) 5, 92, 134, 381, 609
BtgI CCRYGG 2 cut(s) 129, 504
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 2 cut(s) 141, 165
Cac8I GCNNGC 1 cut(s) 242
CciI TCATGA 1 cut(s) 435
CfoI GCGC 3 cut(s) 210, 365, 466
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 5 cut(s) 91, 133, 323, 334, 379
CseI GACGC 2 cut(s) 421, 518
Csp6I GTAC 1 cut(s) 358
CviAII CATG 5 cut(s) 130, 241, 301, 436, 559
CviQI GTAC 1 cut(s) 358
DpnI GATC 3 cut(s) 59, 267, 528
DpnII GATC 3 cut(s) 57, 265, 526
DrdI GACNNNNNNGTC 1 cut(s) 520
DseDI GACNNNNNNGTC 1 cut(s) 520
EciI GGCGGA 2 cut(s) 115, 158
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 323, 334
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 5 cut(s) 133, 244, 304, 439, 562
FaqI GGGAC 2 cut(s) 208, 336
FatI CATG 5 cut(s) 129, 240, 300, 435, 558
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 5 cut(s) 87, 90, 296, 451, 536
FokI GGATG 2 cut(s) 128, 152
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 5 cut(s) 87, 90, 296, 451, 536
FspBI CTAG 1 cut(s) 98
GlaI GCGC 3 cut(s) 209, 364, 465
GluI GCNGC 5 cut(s) 87, 90, 296, 451, 536
HaeIII GGCC 5 cut(s) 5, 92, 134, 381, 609
HapII CCGG 4 cut(s) 305, 316, 429, 617
HgaI GACGC 2 cut(s) 421, 518
HhaI GCGC 3 cut(s) 210, 365, 466
Hin1I GRCGYC 2 cut(s) 432, 521
Hin1II CATG 5 cut(s) 133, 244, 304, 439, 562
Hin6I GCGC 3 cut(s) 208, 363, 464
HinP1I GCGC 3 cut(s) 208, 363, 464
HinfI GANTC 1 cut(s) 290
HpaII CCGG 4 cut(s) 305, 316, 429, 617
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 232, 526
Hpy188III TCNNGA 5 cut(s) 61, 287, 370, 436, 530
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 7 cut(s) 110, 183, 281, 332, 463, 523, 526
HpyAV CCTTC 4 cut(s) 104, 155, 391, 397
HpyCH4IV ACGT 3 cut(s) 108, 356, 521
HpyCH4V TGCA 3 cut(s) 112, 538, 591
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 470
HpySE526I ACGT 3 cut(s) 108, 356, 521
Hsp92I GRCGYC 2 cut(s) 432, 521
Hsp92II CATG 5 cut(s) 133, 244, 304, 439, 562
HspAI GCGC 3 cut(s) 208, 363, 464
KflI GGGWCCC 1 cut(s) 323
Kzo9I GATC 3 cut(s) 57, 265, 526
LmnI GCTCC 2 cut(s) 307, 532
LpnPI CCDG 8 cut(s) 46, 230, 318, 329, 355, 407, 442, 458
Lsp1109I GCAGC 3 cut(s) 307, 437, 522
MaeI CTAG 1 cut(s) 98
MaeII ACGT 3 cut(s) 108, 356, 521
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 59, 267, 528
MboI GATC 3 cut(s) 57, 265, 526
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 312
MmeI TCCRAC 2 cut(s) 255, 504
MroXI GAANNNNTTC 1 cut(s) 119
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 4 cut(s) 305, 316, 429, 617
MspR9I CCNGG 1 cut(s) 618
MvnI CGCG 5 cut(s) 208, 339, 365, 466, 512
MwoI GCNNNNNNNGC 2 cut(s) 301, 470
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 1 cut(s) 129
NdeII GATC 3 cut(s) 57, 265, 526
NlaIII CATG 5 cut(s) 133, 244, 304, 439, 562
NlaIV GGNNCC 2 cut(s) 324, 325
NspI RCATGY 1 cut(s) 244
PaeI GCATGC 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 383
PagI TCATGA 1 cut(s) 435
PcsI WCGNNNNNNNCGW 5 cut(s) 267, 285, 336, 362, 429
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 5 cut(s) 88, 91, 297, 452, 537
Ppu21I YACGTR 1 cut(s) 357
PpuMI RGGWCCY 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 323
PspN4I GGNNCC 2 cut(s) 324, 325
PspPI GGNCC 5 cut(s) 91, 133, 323, 334, 379
PspPPI RGGWCCY 1 cut(s) 323
PspXI VCTCGAGB 1 cut(s) 383
PsrI GAACNNNNNNTAC 2 cut(s) 350, 382
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 312
SatI GCNGC 5 cut(s) 87, 90, 296, 451, 536
Sau3AI GATC 3 cut(s) 57, 265, 526
Sau96I GGNCC 5 cut(s) 91, 133, 323, 334, 379
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 383
SinI GGWCC 2 cut(s) 323, 334
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 383, 599
SmoI CTYRAG 2 cut(s) 383, 599
SphI GCATGC 1 cut(s) 244
SsiI CCGC 6 cut(s) 9, 86, 89, 126, 169, 337
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 3 cut(s) 111, 359, 524
TaqI TCGA 4 cut(s) 105, 312, 345, 384
TauI GCSGC 2 cut(s) 89, 92
TfiI GAWTC 1 cut(s) 290
TseI GCWGC 3 cut(s) 295, 450, 535
TspDTI ATGAA 1 cut(s) 466
TspGWI ACGGA 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 323, 334
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 98
ZraI GACGTC 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.