Rh4CG462800

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016656
Physical Location & Seq
Reverse (-)
69360050 .. 69360562
513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG462800.1

Sequence Viewer

Length: 513 bp
ATGGCTATGGCTTCCTCCTCGACCTCGTACAAAGAGTACGAGATCAGGAAAAGAAACCCAAACCCGAAAGTGGCTGCCCTTTTAGTCATCGACATGCAGAACTACTTCGCCGCCATGGCTAAGCCCATCCTCCAAAACCTCCTCACCACCATCCGCCTCTGCCGACGCGCCTCCATCCCCGTCATCTTCACGCGCCACTCCCACAAGTCCCCCTCCGACTATGGCATGCTGGACGAGTGGTGGAACGGCGACCTCATCTTCGACGGGACTCCCGAGTCCCAGCTGATGCCCGAGCTCGACCGCAGGCCCCACCAGGACCACGTCGTCGACAAGAACACCTACAGCGCCTTCCGCGGCACGGGCCTCGAGGACTATTTGAAGGAGAGGGGCGTCCAGGAGGTCATCGTCACCGGCGTCATGACCAACTTGTGCTGCGAAACGACGGCGCGTTTAGGCCTTCGTCCGGGGCTTCAGAGTGTTCTTTTCTACGGACGCCACTGCCACTTCGGATGA

Protein Analysis

170

Amino Acids

19.37

Weight (kDa)

8.36

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 25 - 161 3.1e-37 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 274, 323
AbsI CCTCGAGG 1 cut(s) 365
AccI GTMKAC 1 cut(s) 327
AccII CGCG 4 cut(s) 168, 193, 354, 448
AciI CCGC 5 cut(s) 111, 154, 301, 352, 354
AcuI CTGAAG 1 cut(s) 455
AcyI GRCGYC 3 cut(s) 390, 414, 493
AfaI GTAC 2 cut(s) 29, 38
AfiI CCNNNNNNNGG 3 cut(s) 70, 358, 463
AgsI TTSAA 1 cut(s) 379
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 312, 393
AluBI AGCT 2 cut(s) 283, 295
AluI AGCT 2 cut(s) 283, 295
Alw21I GWGCWC 1 cut(s) 297
Ama87I CYCGRG 3 cut(s) 272, 290, 365
AoxI GGCC 3 cut(s) 305, 361, 454
ApeKI GCWGC 2 cut(s) 74, 432
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 1 cut(s) 104
AspLEI GCGC 4 cut(s) 170, 195, 347, 448
AspS9I GGNCC 3 cut(s) 306, 316, 361
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 136, 400
AvaI CYCGRG 3 cut(s) 272, 290, 365
AvaII GGWCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 61, 419
BccI CCATC 3 cut(s) 134, 158, 182
BceAI ACGGC 2 cut(s) 262, 459
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 2 cut(s) 314, 395
BcnI CCSGG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 4 cut(s) 75, 111, 355, 433
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 4 cut(s) 76, 112, 356, 434
Bme1390I CCNGG 3 cut(s) 314, 395, 465
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 3 cut(s) 272, 290, 365
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 306, 316, 361
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 3 cut(s) 314, 395, 465
BmsI GCATC 1 cut(s) 276
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 465
BsaHI GRCGYC 3 cut(s) 390, 414, 493
BsaJI CCNNGG 3 cut(s) 114, 352, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 358, 463
Bse118I RCCGGY 1 cut(s) 410
BseBI CCWGG 2 cut(s) 314, 395
BseDI CCNNGG 3 cut(s) 114, 352, 464
BseGI GGATG 3 cut(s) 126, 150, 174
BseLI CCNNNNNNNGG 3 cut(s) 70, 358, 463
BseRI GAGGAG 2 cut(s) 7, 131
BseXI GCAGC 2 cut(s) 61, 419
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 4 cut(s) 168, 193, 354, 448
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 3 cut(s) 307, 363, 456
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 3 cut(s) 272, 290, 365
BsiSI CCGG 2 cut(s) 411, 464
BslFI GGGAC 3 cut(s) 193, 262, 280
BslI CCNNNNNNNGG 3 cut(s) 70, 358, 463
BsmFI GGGAC 3 cut(s) 193, 262, 280
BsnI GGCC 3 cut(s) 307, 363, 456
BsoBI CYCGRG 3 cut(s) 272, 290, 365
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 1 cut(s) 42
Bsp1720I GCTNAGC 1 cut(s) 120
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 5 cut(s) 111, 154, 301, 352, 354
BspANI GGCC 3 cut(s) 307, 363, 456
BspFNI CGCG 4 cut(s) 168, 193, 354, 448
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 3 cut(s) 114, 352, 464
BssMI GATC 1 cut(s) 42
BssNI GRCGYC 3 cut(s) 390, 414, 493
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 314, 395
BstACI GRCGYC 3 cut(s) 390, 414, 493
BstC8I GCNNGC 2 cut(s) 227, 305
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 2 cut(s) 114, 352
BstF5I GGATG 3 cut(s) 126, 150, 174
BstFNI CGCG 4 cut(s) 168, 193, 354, 448
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 4 cut(s) 170, 195, 347, 448
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 3 cut(s) 116, 351, 360
BstNI CCWGG 2 cut(s) 314, 395
BstNSI RCATGY 2 cut(s) 97, 229
BstSCI CCNGG 3 cut(s) 312, 393, 463
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 4 cut(s) 168, 193, 354, 448
BstV1I GCAGC 2 cut(s) 61, 419
BsuRI GGCC 3 cut(s) 307, 363, 456
BtgI CCRYGG 2 cut(s) 114, 352
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 3 cut(s) 126, 150, 174
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 2 cut(s) 227, 305
CciI TCATGA 1 cut(s) 417
CfoI GCGC 4 cut(s) 170, 195, 347, 448
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 3 cut(s) 306, 316, 361
Cfr42I CCGCGG 1 cut(s) 355
CseI GACGC 4 cut(s) 174, 379, 403, 501
Csp6I GTAC 2 cut(s) 28, 37
CviAII CATG 4 cut(s) 94, 115, 226, 418
CviQI GTAC 2 cut(s) 28, 37
DdeI CTNAG 1 cut(s) 120
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
DrdI GACNNNNNNGTC 2 cut(s) 274, 323
DseDI GACNNNNNNGTC 2 cut(s) 274, 323
EciI GGCGGA 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 455
Eco88I CYCGRG 3 cut(s) 272, 290, 365
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 312, 393
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 4 cut(s) 97, 118, 229, 421
FaiI YATR 6 cut(s) 8, 95, 116, 222, 227, 419
FaqI GGGAC 3 cut(s) 193, 262, 280
FatI CATG 4 cut(s) 93, 114, 225, 417
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 4 cut(s) 75, 111, 355, 433
FokI GGATG 3 cut(s) 113, 137, 161
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 4 cut(s) 75, 111, 355, 433
GlaI GCGC 4 cut(s) 169, 194, 346, 447
GluI GCNGC 4 cut(s) 75, 111, 355, 433
GsaI CCCAGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 3 cut(s) 307, 363, 456
HapII CCGG 2 cut(s) 411, 464
HgaI GACGC 4 cut(s) 174, 379, 403, 501
HhaI GCGC 4 cut(s) 170, 195, 347, 448
Hin1I GRCGYC 3 cut(s) 390, 414, 493
Hin1II CATG 4 cut(s) 97, 118, 229, 421
Hin6I GCGC 4 cut(s) 168, 193, 345, 446
HinP1I GCGC 4 cut(s) 168, 193, 345, 446
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 2 cut(s) 268, 275
HpaII CCGG 2 cut(s) 411, 464
HphI GGTGA 2 cut(s) 136, 400
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 3 cut(s) 217, 474, 509
Hpy188III TCNNGA 3 cut(s) 46, 272, 418
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 5 cut(s) 168, 266, 326, 329, 445
HpyAV CCTTC 3 cut(s) 358, 373, 467
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 351, 360
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 3 cut(s) 390, 414, 493
Hsp92II CATG 4 cut(s) 97, 118, 229, 421
HspAI GCGC 4 cut(s) 168, 193, 345, 446
KspI CCGCGG 1 cut(s) 355
Kzo9I GATC 1 cut(s) 42
Lsp1109I GCAGC 2 cut(s) 61, 419
LweI GCATC 1 cut(s) 276
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 406
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 2 cut(s) 178, 250
MhlI GDGCHC 1 cut(s) 297
MlyI GAGTC 2 cut(s) 262, 284
MmeI TCCRAC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 92
MspA1I CMGCKG 2 cut(s) 283, 354
MspI CCGG 2 cut(s) 411, 464
MspR9I CCNGG 3 cut(s) 314, 395, 465
MvaI CCWGG 2 cut(s) 314, 395
MvnI CGCG 4 cut(s) 168, 193, 354, 448
MwoI GCNNNNNNNGC 3 cut(s) 116, 351, 360
NciI CCSGG 1 cut(s) 465
NcoI CCATGG 1 cut(s) 114
NdeII GATC 1 cut(s) 42
NlaIII CATG 4 cut(s) 97, 118, 229, 421
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 2 cut(s) 97, 229
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 365
PagI TCATGA 1 cut(s) 417
PceI AGGCCT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 411
PdmI GAANNNNTTC 1 cut(s) 104
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 4 cut(s) 76, 112, 356, 434
PleI GAGTC 2 cut(s) 262, 283
PpsI GAGTC 2 cut(s) 262, 283
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 312, 393
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 2 cut(s) 312, 393
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 3 cut(s) 306, 316, 361
PspXI VCTCGAGB 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 2 cut(s) 29, 38
RsaNI GTAC 2 cut(s) 28, 37
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 297
SacII CCGCGG 1 cut(s) 355
SalI GTCGAC 1 cut(s) 326
SatI GCNGC 4 cut(s) 75, 111, 355, 433
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 3 cut(s) 306, 316, 361
SchI GAGTC 2 cut(s) 262, 284
ScrFI CCNGG 3 cut(s) 314, 395, 465
SduI GDGCHC 1 cut(s) 297
SetI ASST 8 cut(s) 26, 141, 255, 285, 297, 324, 341, 402
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 340
Sfr274I CTCGAG 1 cut(s) 365
Sfr303I CCGCGG 1 cut(s) 355
SgrAI CRCCGGYG 1 cut(s) 410
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SphI GCATGC 1 cut(s) 229
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 5 cut(s) 111, 154, 301, 352, 354
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 312, 393, 463
StyI CCWWGG 1 cut(s) 114
TaiI ACGT 1 cut(s) 324
TaqI TCGA 6 cut(s) 20, 90, 261, 297, 327, 366
TauI GCSGC 2 cut(s) 113, 357
TscAI CASTG 1 cut(s) 503
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 2 cut(s) 74, 432
Tsp45I GTSAC 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 1 cut(s) 503
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 2 cut(s) 97, 229
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.