Rw4G037320

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016656
Physical Location & Seq
Reverse (-)
63994887 .. 63995516
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037320.1

Sequence Viewer

Length: 630 bp
ATGGCTACGGCCATGGCTTCCTCCTCGACCTCGTACAAAGAGTACGAGATCAGAAAAAGAAACCCAAACCCGAAAGTGGCTGCCCTTTTAGTCATCGACATGCAGAACTACTTCGCCGCCATGGCTAAGCCTATCCTCCAAAACCTCCTCACCACCATCCGTATCTGCCGACGCGCTTCCATCCCCGTCATCTTCACGCGCCACTCCCAGAAGTCCCCCTCCGACTATGGCATGCTGGACGAGTGGTGGAACGGCGACCTCGTCTTCGACGGGACTCCCGAGTCCCAGCTGATGCCCGAGCTCGACCGCAGGCCCCACCAGGACCACCTCGTCGACAACAAGAACACCTACAGCGCCTTCCGCGGCACTGGCCTCGAGGACTATTTGAAGGAGAGGGGCGTCCAGGAGGTCATCGTCACCGGCGTCATGACCAACTTGTGCTGCGAAACGACGGCGCGTGAGGCCTTCGTCCGGGGCTTCAGAGTGTTCTTTTCTACGGACGCCACTGCCACTTCGGATGAAGAGCTGCACGAGGCCACCTTGAAGAACTGGCCTATGGCTTTGCCTACCTCGTCGATTGCAACAGACTTGATCAAGCTCTTTAGTTTCTTCTGCAATTTCAGTTATTAA

Protein Analysis

209

Amino Acids

23.76

Weight (kDa)

5.83

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 27 - 183 3.3e-44 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 280, 329
AbsI CCTCGAGG 1 cut(s) 374
AccI GTMKAC 1 cut(s) 333
AccII CGCG 4 cut(s) 174, 199, 363, 457
AciI CCGC 4 cut(s) 117, 307, 361, 363
AcoI YGGCCR 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 463
AcyI GRCGYC 3 cut(s) 399, 423, 501
AfaI GTAC 2 cut(s) 35, 44
AfiI CCNNNNNNNGG 2 cut(s) 76, 471
AgsI TTSAA 2 cut(s) 388, 544
AjnI CCWGG 2 cut(s) 318, 402
AluBI AGCT 4 cut(s) 289, 301, 526, 598
AluI AGCT 4 cut(s) 289, 301, 526, 598
Alw21I GWGCWC 1 cut(s) 303
Ama87I CYCGRG 3 cut(s) 278, 296, 374
AoxI GGCC 6 cut(s) 9, 311, 370, 462, 534, 551
ApeKI GCWGC 3 cut(s) 80, 441, 526
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 4 cut(s) 176, 201, 356, 457
AspS9I GGNCC 2 cut(s) 312, 322
AsuC2I CCSGG 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 142, 409
AvaI CYCGRG 3 cut(s) 278, 296, 374
AvaII GGWCC 1 cut(s) 322
BanII GRGCYC 1 cut(s) 303
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 67, 428, 513
BccI CCATC 2 cut(s) 164, 188
BceAI ACGGC 3 cut(s) 24, 268, 468
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 2 cut(s) 320, 404
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 473
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 357
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 5 cut(s) 81, 117, 364, 442, 527
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 5 cut(s) 82, 118, 365, 443, 528
Bme1390I CCNGG 3 cut(s) 320, 404, 473
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 3 cut(s) 278, 296, 374
BmgT120I GGNCC 2 cut(s) 312, 322
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 3 cut(s) 320, 404, 473
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 256
Bpu1102I GCTNAGC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 473
BsaHI GRCGYC 3 cut(s) 399, 423, 501
BsaJI CCNNGG 4 cut(s) 12, 120, 361, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 471
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 2 cut(s) 373, 554
BseBI CCWGG 2 cut(s) 320, 404
BseDI CCNNGG 4 cut(s) 12, 120, 361, 472
BseGI GGATG 3 cut(s) 156, 180, 523
BseLI CCNNNNNNNGG 2 cut(s) 76, 471
BseNI ACTGG 2 cut(s) 373, 554
BseRI GAGGAG 2 cut(s) 13, 137
BseXI GCAGC 3 cut(s) 67, 428, 513
BseYI CCCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 4 cut(s) 174, 199, 363, 457
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 6 cut(s) 11, 313, 372, 464, 536, 553
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 3 cut(s) 278, 296, 374
BsiSI CCGG 2 cut(s) 420, 472
BslFI GGGAC 3 cut(s) 199, 268, 286
BslI CCNNNNNNNGG 2 cut(s) 76, 471
BsmFI GGGAC 3 cut(s) 199, 268, 286
BsnI GGCC 6 cut(s) 11, 313, 372, 464, 536, 553
BsoBI CYCGRG 3 cut(s) 278, 296, 374
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 2 cut(s) 48, 591
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 2 cut(s) 12, 120
BspACI CCGC 4 cut(s) 117, 307, 361, 363
BspANI GGCC 6 cut(s) 11, 313, 372, 464, 536, 553
BspFNI CGCG 4 cut(s) 174, 199, 363, 457
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 314
BspQI GCTCTTC 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 2 cut(s) 373, 554
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 4 cut(s) 12, 120, 361, 472
BssMI GATC 2 cut(s) 48, 591
BssNI GRCGYC 3 cut(s) 399, 423, 501
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 12, 120
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 2 cut(s) 320, 404
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 3 cut(s) 399, 423, 501
BstC8I GCNNGC 2 cut(s) 233, 311
BstDEI CTNAG 1 cut(s) 126
BstDSI CCRYGG 3 cut(s) 12, 120, 361
BstF5I GGATG 3 cut(s) 156, 180, 523
BstFNI CGCG 4 cut(s) 174, 199, 363, 457
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 4 cut(s) 176, 201, 356, 457
BstKTI GATC 2 cut(s) 51, 594
BstMBI GATC 2 cut(s) 48, 591
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 4 cut(s) 122, 360, 369, 461
BstNI CCWGG 2 cut(s) 320, 404
BstNSI RCATGY 2 cut(s) 103, 235
BstSCI CCNGG 3 cut(s) 318, 402, 471
BstSFI CTRYAG 1 cut(s) 349
BstUI CGCG 4 cut(s) 174, 199, 363, 457
BstV1I GCAGC 3 cut(s) 67, 428, 513
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 6 cut(s) 11, 313, 372, 464, 536, 553
BtgI CCRYGG 3 cut(s) 12, 120, 361
BtsCI GGATG 3 cut(s) 156, 180, 523
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 366, 504
Cac8I GCNNGC 2 cut(s) 233, 311
CciI TCATGA 1 cut(s) 426
CfoI GCGC 4 cut(s) 176, 201, 356, 457
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 2 cut(s) 312, 322
Cfr42I CCGCGG 1 cut(s) 364
CseI GACGC 4 cut(s) 180, 388, 412, 509
Csp6I GTAC 2 cut(s) 34, 43
CviAII CATG 5 cut(s) 13, 100, 121, 232, 427
CviQI GTAC 2 cut(s) 34, 43
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 50, 593
DpnII GATC 2 cut(s) 48, 591
DrdI GACNNNNNNGTC 2 cut(s) 280, 329
DseDI GACNNNNNNGTC 2 cut(s) 280, 329
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 12, 120
Eco147I AGGCCT 1 cut(s) 464
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 463
Eco88I CYCGRG 3 cut(s) 278, 296, 374
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 318, 402
EcoT14I CCWWGG 2 cut(s) 12, 120
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 12, 120
FaeI CATG 5 cut(s) 16, 103, 124, 235, 430
FaiI YATR 7 cut(s) 14, 101, 122, 228, 233, 428, 557
FaqI GGGAC 3 cut(s) 199, 268, 286
FatI CATG 5 cut(s) 12, 99, 120, 231, 426
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 81, 117, 364, 442, 527
FokI GGATG 3 cut(s) 143, 167, 530
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 5 cut(s) 81, 117, 364, 442, 527
GlaI GCGC 4 cut(s) 175, 200, 355, 456
GluI GCNGC 5 cut(s) 81, 117, 364, 442, 527
GsaI CCCAGC 1 cut(s) 289
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 6 cut(s) 11, 313, 372, 464, 536, 553
HapII CCGG 2 cut(s) 420, 472
HgaI GACGC 4 cut(s) 180, 388, 412, 509
HhaI GCGC 4 cut(s) 176, 201, 356, 457
Hin1I GRCGYC 3 cut(s) 399, 423, 501
Hin1II CATG 5 cut(s) 16, 103, 124, 235, 430
Hin6I GCGC 4 cut(s) 174, 199, 354, 455
HinP1I GCGC 4 cut(s) 174, 199, 354, 455
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 2 cut(s) 274, 281
HpaII CCGG 2 cut(s) 420, 472
HphI GGTGA 2 cut(s) 142, 409
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 53, 223, 482, 517
Hpy188III TCNNGA 2 cut(s) 278, 427
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 5 cut(s) 174, 272, 335, 454, 577
HpyAV CCTTC 3 cut(s) 367, 382, 475
HpyCH4V TGCA 4 cut(s) 103, 529, 581, 615
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 360, 369, 461
HpyF3I CTNAG 1 cut(s) 126
Hsp92I GRCGYC 3 cut(s) 399, 423, 501
Hsp92II CATG 5 cut(s) 16, 103, 124, 235, 430
HspAI GCGC 4 cut(s) 174, 199, 354, 455
Ksp22I TGATCA 1 cut(s) 591
KspI CCGCGG 1 cut(s) 364
Kzo9I GATC 2 cut(s) 48, 591
LguI GCTCTTC 1 cut(s) 516
Lsp1109I GCAGC 3 cut(s) 67, 428, 513
LweI GCATC 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 415
MalI GATC 2 cut(s) 50, 593
MboI GATC 2 cut(s) 48, 591
MboII GAAGA 5 cut(s) 184, 256, 533, 556, 601
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 616
MlyI GAGTC 2 cut(s) 268, 290
MmeI TCCRAC 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 1 cut(s) 628
MslI CAYNNNNRTG 1 cut(s) 98
MspA1I CMGCKG 2 cut(s) 289, 363
MspI CCGG 2 cut(s) 420, 472
MspR9I CCNGG 3 cut(s) 320, 404, 473
MvaI CCWGG 2 cut(s) 320, 404
MvnI CGCG 4 cut(s) 174, 199, 363, 457
MwoI GCNNNNNNNGC 4 cut(s) 122, 360, 369, 461
NciI CCSGG 1 cut(s) 473
NcoI CCATGG 2 cut(s) 12, 120
NdeII GATC 2 cut(s) 48, 591
NlaIII CATG 5 cut(s) 16, 103, 124, 235, 430
NlaIV GGNNCC 1 cut(s) 314
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 2 cut(s) 103, 235
PaeI GCATGC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 374
PagI TCATGA 1 cut(s) 426
PceI AGGCCT 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 276, 420
PdmI GAANNNNTTC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 5 cut(s) 82, 118, 365, 443, 528
PleI GAGTC 2 cut(s) 268, 289
PpsI GAGTC 2 cut(s) 268, 289
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 318, 402
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 2 cut(s) 318, 402
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 2 cut(s) 312, 322
PspXI VCTCGAGB 1 cut(s) 374
PsyI GACNNNGTC 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 2 cut(s) 35, 44
RsaNI GTAC 2 cut(s) 34, 43
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 303
SacII CCGCGG 1 cut(s) 364
SalI GTCGAC 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 628
SatI GCNGC 5 cut(s) 81, 117, 364, 442, 527
Sau3AI GATC 2 cut(s) 48, 591
Sau96I GGNCC 2 cut(s) 312, 322
SchI GAGTC 2 cut(s) 268, 290
ScrFI CCNGG 3 cut(s) 320, 404, 473
SduI GDGCHC 1 cut(s) 303
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 349
Sfr274I CTCGAG 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 364
SgrAI CRCCGGYG 1 cut(s) 419
SgrBI CCGCGG 1 cut(s) 364
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 235
Sse9I AATT 1 cut(s) 616
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 4 cut(s) 117, 307, 361, 363
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 318, 402, 471
StyI CCWWGG 2 cut(s) 12, 120
TaqI TCGA 7 cut(s) 26, 96, 267, 303, 333, 375, 575
TasI AATT 1 cut(s) 616
TauI GCSGC 2 cut(s) 119, 366
Tru1I TTAA 1 cut(s) 628
Tru9I TTAA 1 cut(s) 628
TscAI CASTG 2 cut(s) 373, 511
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 80, 441, 526
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 534
TspGWI ACGGA 2 cut(s) 149, 512
TspRI CASTG 2 cut(s) 373, 511
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 322
XceI RCATGY 2 cut(s) 103, 235
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.