AT5G23230

nicotinamidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016656
Physical Location & Seq
Reverse (-)
7825858 .. 7826611
754 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23230.1

Sequence Viewer

Length: 597 bp
ATGGCTTCTTCATCATCGAGAACGTACGAGACACGAAAGCGAGAGCCAAATCCTAAAATCGCAGCTCTTCTCGTCATCGATATGCAGAATCACTTCTACTCTATGGCTGAACCAATCCTCCAAAACGCTCTCACCACCATCGACATCTGCCGACGCGCTTCAATCCCCGTATTCTTCACGCGCCACAACCACAAATCCCCAACCGACCACGGCATGCTCGGAGAGTGGTGGAACGGCGATCTAATCCTCGACGGAACCACTGATTCCGAAATCATCCCGGAAATCAATCGCCAGGTCACCGGACCAGACGAAATCGTGGAGAAGAGCACGTACAGTGCGTTTAACAACACGCACCTTCAGGAGAAGCTGGACAAGATCGGAGTGAAGGAGGTGATCGTTATCGGAGTGATGACGAACCTATGCTGTGAGACGACGGCGCGTGAAGCGTTTGTAAAGGGGTTTAGGGTTTTTTTCTCGACGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACTCTAATGAATCTCGCGTATGGCTTTGCTTATCTCGTCGATTGCGATAGACTCCGGCGAGGTCTACTCAGTAGTTAA

Protein Analysis

198

Amino Acids

22.32

Weight (kDa)

5.51

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 21 - 187 1.3e-43 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016656)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23220 AT5G23230
malus_domestica MD16G1003500.v1.1
prunus_persica Prupe.1G348500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0446811
rosa_laevigata RLG00000005628
rosa_multiflora Rmu_co8267649.1_g000001 Rmu_sc0001753.1_g000021
rosa_rugosa Rorug04G0374900
rosa_samantha Rh4AG436400 Rh4BG445500 Rh4CG462800 Rh4DG444200
rosa_wichuraiana Rw4G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 583
AccII CGCG 5 cut(s) 156, 181, 439, 485, 536
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 2 cut(s) 26, 332
AgsI TTSAA 1 cut(s) 162
AjnI CCWGG 1 cut(s) 291
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
AluBI AGCT 3 cut(s) 65, 367, 508
AluI AGCT 3 cut(s) 65, 367, 508
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 23, 422
ApeKI GCWGC 1 cut(s) 62
Asp700I GAANNNNTTC 2 cut(s) 92, 507
AspLEI GCGC 3 cut(s) 158, 183, 439
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 124, 289, 403
AvaII GGWCC 1 cut(s) 302
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BauI CACGAG 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 74
BccI CCATC 1 cut(s) 146
BceAI ACGGC 3 cut(s) 226, 250, 450
BciT130I CCWGG 1 cut(s) 293
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 23, 422
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 278, 293
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 278, 293
BplI GAGNNNNNCTC 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 278
Bsa29I ATCGAT 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 273
BseJI GATNNNNATC 1 cut(s) 398
BseXI GCAGC 1 cut(s) 74
Bsh1236I CGCG 5 cut(s) 156, 181, 439, 485, 536
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 3 cut(s) 278, 300, 574
BsiWI CGTACG 1 cut(s) 24
BsmAI GTCTC 2 cut(s) 23, 422
BsmBI CGTCTC 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 3 cut(s) 238, 375, 393
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 5 cut(s) 156, 181, 439, 485, 536
BspLI GGNNCC 1 cut(s) 256
BspQI GCTCTTC 3 cut(s) 72, 317, 498
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 3 cut(s) 238, 375, 393
BssSI CACGAG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 512
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 335, 496
Bst6I CTCTTC 3 cut(s) 72, 317, 498
BstBAI YACGTR 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 587
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 5 cut(s) 156, 181, 439, 485, 536
BstHHI GCGC 3 cut(s) 158, 183, 439
BstKTI GATC 3 cut(s) 241, 378, 396
BstMAI GTCTC 2 cut(s) 23, 422
BstMBI GATC 3 cut(s) 238, 375, 393
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 276, 291
BstUI CGCG 5 cut(s) 156, 181, 439, 485, 536
BstV1I GCAGC 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 258, 340
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 3 cut(s) 158, 183, 439
Cfr13I GGNCC 1 cut(s) 302
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 2 cut(s) 162, 491
Csp6I GTAC 2 cut(s) 25, 331
CviAII CATG 1 cut(s) 214
CviJI RGCY 8 cut(s) 5, 46, 65, 107, 367, 508, 518, 543
CviKI_1 RGCY 8 cut(s) 5, 46, 65, 107, 367, 508, 518, 543
CviQI GTAC 2 cut(s) 25, 331
DdeI CTNAG 1 cut(s) 587
DpnI GATC 3 cut(s) 240, 377, 395
DpnII GATC 3 cut(s) 238, 375, 393
Eam1104I CTCTTC 3 cut(s) 72, 317, 498
EarI CTCTTC 3 cut(s) 72, 317, 498
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 341
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 1 cut(s) 217
FaiI YATR 5 cut(s) 83, 104, 215, 421, 540
FatI CATG 1 cut(s) 213
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 1 cut(s) 63
GlaI GCGC 3 cut(s) 157, 182, 438
GluI GCNGC 1 cut(s) 63
HapII CCGG 3 cut(s) 278, 300, 574
HgaI GACGC 2 cut(s) 162, 491
HhaI GCGC 3 cut(s) 158, 183, 439
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 3 cut(s) 156, 181, 437
HinP1I GCGC 3 cut(s) 156, 181, 437
HinfI GANTC 4 cut(s) 88, 263, 529, 570
HpaII CCGG 3 cut(s) 278, 300, 574
HphI GGTGA 3 cut(s) 124, 289, 403
Hpy166II GTNNAC 1 cut(s) 584
Hpy188I TCNGA 4 cut(s) 221, 268, 380, 404
Hpy188III TCNNGA 4 cut(s) 18, 359, 475, 512
Hpy8I GTNNAC 1 cut(s) 584
Hpy99I CGWCG 6 cut(s) 156, 254, 436, 481, 496, 560
HpyAV CCTTC 2 cut(s) 365, 379
HpyCH4III ACNGT 2 cut(s) 335, 496
HpyCH4IV ACGT 2 cut(s) 23, 329
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 2 cut(s) 23, 329
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 3 cut(s) 156, 181, 437
Kzo9I GATC 3 cut(s) 238, 375, 393
LguI GCTCTTC 3 cut(s) 72, 317, 498
LpnPI CCDG 8 cut(s) 278, 291, 305, 313, 318, 344, 353, 587
Lsp1109I GCAGC 1 cut(s) 74
MaeII ACGT 2 cut(s) 23, 329
MaeIII GTNAC 1 cut(s) 295
MalI GATC 3 cut(s) 240, 377, 395
MboI GATC 3 cut(s) 238, 375, 393
MboII GAAGA 4 cut(s) 59, 166, 334, 515
MhlI GDGCHC 1 cut(s) 329
MlyI GAGTC 1 cut(s) 564
MnlI CCTC 5 cut(s) 128, 257, 382, 508, 572
MroXI GAANNNNTTC 2 cut(s) 92, 507
MseI TTAA 3 cut(s) 342, 498, 595
MslI CAYNNNNRTG 1 cut(s) 80
MspI CCGG 3 cut(s) 278, 300, 574
MspR9I CCNGG 2 cut(s) 278, 293
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 5 cut(s) 156, 181, 439, 485, 536
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 278
NdeII GATC 3 cut(s) 238, 375, 393
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PciSI GCTCTTC 3 cut(s) 72, 317, 498
PcsI WCGNNNNNNNCGW 3 cut(s) 335, 482, 561
PdmI GAANNNNTTC 2 cut(s) 92, 507
PfeI GAWTC 3 cut(s) 88, 263, 529
Pfl23II CGTACG 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
Ppu21I YACGTR 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 291
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 291
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 302
RsaI GTAC 2 cut(s) 26, 332
RsaNI GTAC 2 cut(s) 25, 331
RseI CAYNNNNRTG 1 cut(s) 80
SapI GCTCTTC 3 cut(s) 72, 317, 498
SaqAI TTAA 3 cut(s) 342, 498, 595
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 238, 375, 393
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 2 cut(s) 278, 293
SduI GDGCHC 1 cut(s) 329
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 80
SphI GCATGC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 276, 291
TaaI ACNGT 2 cut(s) 335, 496
TaiI ACGT 2 cut(s) 26, 332
TaqI TCGA 6 cut(s) 17, 78, 141, 249, 476, 558
TfiI GAWTC 3 cut(s) 88, 263, 529
Tru1I TTAA 3 cut(s) 342, 498, 595
Tru9I TTAA 3 cut(s) 342, 498, 595
TscAI CASTG 2 cut(s) 265, 340
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 516, 542
TspGWI ACGGA 2 cut(s) 267, 494
TspRI CASTG 2 cut(s) 265, 340
VpaK11BI GGWCC 1 cut(s) 302
XceI RCATGY 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 2 cut(s) 92, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.